CN113999325A - Rice bran fermented polysaccharide, preparation and application - Google Patents
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Abstract
The invention discloses a rice bran fermented polysaccharide, and preparation and application thereof. The rice bran fermented polysaccharide is composed of L-arabinose, D-mannose and D-glucose according to the molar ratio of 0.5:4.7:5.0, the biological activity of the rice bran fermented polysaccharide is improved after solid state fermentation, compared with natural rice bran polysaccharide, the rice bran fermented polysaccharide has the advantages of obvious advantages, high solubility, good biological activity and the like, the tumor growth of non-small cell lung cancer can be effectively inhibited, and the inhibition rate is up to over 86%. Compared with liquid fermentation, the preparation method has the advantages of simple process, convenient operation, low operation cost, no generation of fermentation waste liquid, abundant side products except polysaccharide, and high added value of products, and can be used for mass production.
Description
Technical Field
The invention relates to the technical field of medicines and health-care foods. More particularly relates to rice bran fermented polysaccharide, preparation and application.
Background
China is a big country for rice production, and the rice processing by-products have rich resources. The rice bran is a main byproduct generated in the process of processing rice into rice, is rich in nutritional ingredients such as lipid, carbohydrate, protein, vitamin E, oryzanol and the like, and has high application value. The defatted rice bran obtained by leaching and defatting the rice bran is called rice bran meal, the rice bran meal contains more than 50% of dietary fiber, 20% -30% of starch, about 20% of protein and about 10% of calcium phytate, the protein and the dietary fiber are higher in content, and the shelf life is longer than that of the rice bran. The rice bran polysaccharide is an important functional component in the rice bran meal, is heteroglycan with a complex structure, and has various effects of resisting tumors, improving immunity, reducing blood sugar, reducing blood fat, resisting bacteria, resisting radiation, preserving freshness and the like. The function of the rice bran polysaccharide has an important relationship with the structure of the rice bran polysaccharide, and certain components in the natural rice bran polysaccharide have the problems of large molecular weight, small solubility, no exposure of active glycosyl and the like, so that the natural rice bran polysaccharide cannot well play an active role, and therefore, the rice bran polysaccharide can be properly modified by a physical, chemical or biological method to improve the biological activity of the rice bran polysaccharide.
The microbial solid state fermentation technology has the advantages of high product concentration, low equipment requirement, low energy consumption, no emission and the like, and is an excellent method for modifying the hemicellulose polysaccharide in the rice bran.
Therefore, it is very important to develop a rice bran fermented polysaccharide prepared by solid state fermentation technology to overcome many problems of natural rice bran polysaccharides.
Disclosure of Invention
The invention aims to provide rice bran fermented polysaccharide, which is modified by a solid state fermentation technology, improves the biological activity, has the advantages of high solubility, good biological activity and the like, and has obvious advantages compared with natural rice bran polysaccharide.
The second object of the present invention is to provide a method for preparing the above fermented rice bran polysaccharide. The preparation method has simple process, the strain selection has wide applicability to common large edible fungi, lactobacillus, mould fungi and the like, the polysaccharide can be used for mass production, the purity of the polysaccharide is high, the byproducts are rich except the polysaccharide, the added value of the product is high, compared with liquid fermentation, the operation is simple, the operation cost is low, and no fermentation waste liquid is generated.
The third purpose of the invention is to provide the application of the rice bran fermented polysaccharide in the medical and health-care products.
In order to achieve the first purpose, the invention adopts the following technical scheme:
the invention discloses rice bran fermented polysaccharide which is characterized by having a structural formula shown in the specification
Further, the weight average molecular weight of the rice bran fermented polysaccharide is 2.58 × 104Da, wherein the rice bran fermented polysaccharide consists of L-arabinose, D-mannose and D-glucose according to the molar ratio of 0.5:4.7: 5.0.
The rice bran fermented polysaccharide provided by the invention is a novel fermented polysaccharide obtained by a microbial solid state fermentation technology, improves the biological activity, has obvious advantages compared with natural rice bran polysaccharide, has higher total fat and total polysaccharide content than unfermented rice bran, and shows that the rice bran fermented polysaccharide is composed of L-arabinose, D-mannose and D-glucose according to the molar ratio of 0.5:4.7:5.0, the D-glucose and the L-arabinose are in an alpha configuration, and the D-mannose is in a beta configuration by HPLC-ELSD analysis.
In order to achieve the second purpose, the invention adopts the following technical scheme:
the invention discloses a preparation method of the rice bran fermented polysaccharide, which comprises the following steps:
(1) solid state fermentation: inoculating the ganoderma lucidum seed solution into the rice bran raw material for fermentation culture, drying and crushing the rice bran raw material after the fermentation culture to obtain fermented rice bran;
(2) extracting rice bran fermentation crude polysaccharide: adding fermented rice bran into petroleum ether for extraction, performing suction filtration, adding filter residue into ethanol solution for extraction, performing suction filtration, adding filter residue into water for water extraction to obtain polysaccharide water extract, and performing enzymolysis treatment to obtain enzymolysis water extract; then concentrating the enzymatic water extract to obtain an enzymatic water extract concentrated solution, adding absolute ethyl alcohol, standing, centrifuging, taking precipitate, and drying to obtain rice bran fermented crude polysaccharide;
(3) obtaining rice bran fermented polysaccharide: purifying the crude rice bran fermented polysaccharide by using DEAE ion exchange resin, wherein the eluent is water, further purifying the eluent by using a gel exclusion chromatographic column, and freeze-drying to obtain the rice bran fermented polysaccharide;
wherein, the ganoderma lucidum mycelium related to the invention has the CGMCC number of 5.616 and is purchased from China general microbiological culture Collection center; the concentration of the ganoderma lucidum seed liquid is 10-20 mg/mL; the inoculation amount of the ganoderma lucidum seed liquid is 2-5% of the mass of the rice bran raw material.
In the present application, the ganoderma lucidum mycelia belong to the genus ganoderma lucidum, and other ganoderma lucidum fungi which can be used in food can be selected as fermentation strains for the solid-state fermentation process of the present invention, such as ganoderma lucidum, ganoderma sinensis or ganoderma tsugae.
In a specific embodiment, the rice bran material is required to have a certain water content in the actual preparation, and can be supplemented by spraying or directly adding, uniformly mixing and sterilizing.
Further, the ganoderma lucidum seed solution is obtained by inoculating activated ganoderma lucidum mycelia into a liquid culture medium for amplification culture; preferably, the activation is carried out for 1-10 days at 25-30 ℃ in a PDA culture medium; preferably, the amplification culture is carried out at 25-30 ℃ and 50-300 r/min.
In a specific embodiment, the liquid culture medium is potato dextrose liquid culture medium, and comprises the following components: 1% glucose, 0.3% potato extract, 0.2% KH2PO4、0.1％MgSO4﹒7H2O。
Further, the fermentation culture is carried out for 3-15 days at 25-30 ℃.
Further, the ratio of the fermented rice bran to the petroleum ether is 1g to 2-6 ml, and the extraction time is 10-40 min.
Further, the ratio of the fermented rice bran to the ethanol solution is 1g to 2-6 ml, and the extraction time is 10-40 min.
Further, the ethanol solution is 70-80% by volume; preferably, it is 75% ethanol solution by volume.
Further, the ratio of the fermented rice bran to water is 1g to 2-10 ml, the extraction times are 2-5 times, and the extraction time is 20-40 min.
Further, the enzymolysis treatment is to add 1-5% of amylase and 1-5% of glucoamylase into the polysaccharide water extract, carry out water bath at 40-65 ℃ for 1-12 hours, and then add 1-5% of trypsin, carry out water bath at 40-65 ℃ for 1-12 hours.
Further, the volume ratio of the absolute ethyl alcohol to the enzymolysis water extraction concentrated solution is 2-5: 1.
Further, the concentration is carried out until the density is 1.2-1.3 g/mL.
In a specific embodiment, the standing condition is that the mixture is stored for 12 hours at 4 ℃; the centrifugation condition is 4000r/min, and the centrifugation is 20 min; the freeze drying conditions in the step (5) are that the temperature of a cold trap is less than or equal to minus 60 ℃ and the vacuum degree is less than or equal to 10 Pa.
In order to achieve the third purpose, the invention discloses an application of the rice bran fermented polysaccharide in preparing a medicament for treating non-small cell lung cancer.
The rice bran fermented polysaccharide prepared by the invention is orally intragastrically injected into tumor-bearing mice suffering from H1299 human non-small cell lung cancer, and the tumor body change within 6 weeks is tested, so that the results show that the rice bran fermented polysaccharide can obviously inhibit the tumor body growth, the inhibition rate reaches over 86 percent, and the tumor body slices show that the cancer cells have the phenomenon of atrophy and apoptosis.
The invention has the following beneficial effects:
the invention discloses a rice bran fermented polysaccharide, and preparation and application thereof. The rice bran fermented polysaccharide is composed of L-arabinose, D-mannose and D-glucose according to the molar ratio of 0.5:4.7:5.0, the biological activity of the rice bran fermented polysaccharide is improved after solid state fermentation, compared with natural rice bran polysaccharide, the rice bran fermented polysaccharide has the advantages of obvious advantages, high solubility, good biological activity and the like, the tumor growth of non-small cell lung cancer can be effectively inhibited, and the inhibition rate is up to over 86%. Compared with liquid fermentation, the preparation method has the advantages of simple process, convenient operation, low operation cost, no generation of fermentation waste liquid, abundant side products except polysaccharide, and high added value of products, and can be used for mass production.
Drawings
The following describes embodiments of the present invention in further detail with reference to the accompanying drawings.
FIG. 1 shows a schematic diagram of a rice bran fermented polysaccharide prepared in example 1.
FIG. 2 is a graph showing the comparison of the total fat, total polysaccharide, and total phenol content of the rice bran fermented polysaccharides prepared in example 1 with that of unfermented rice bran.
FIG. 3 shows the fermented rice bran polysaccharide prepared in example 11H-NMR chart.
FIG. 4 shows the fermented rice bran polysaccharide prepared in example 113C-NMR chart.
FIG. 5 shows HSQC profiles of the rice bran fermented polysaccharides prepared in example 1.
FIG. 6 is an infrared spectrum of the fermented rice bran polysaccharide prepared in example 1.
FIG. 7 shows a UV-Vis spectrophotometric chart of the rice bran fermented polysaccharide prepared in example 1 after complexing with Congo red.
FIG. 8 is a chart showing a circular dichroism spectrum of the rice bran fermented polysaccharide prepared in example 1 in a 0.1g/ml NaOH solution.
FIG. 9 shows a graph comparing tumor size during 6 weeks of feeding tumor-bearing mice to the experimental and control groups.
FIG. 10 shows a comparison of tumor body images extracted from the experimental group and the control group.
FIG. 11 shows a comparison of HE sections of tumor bodies of tumor-bearing mice of the experimental group and the control group.
Detailed Description
In order to more clearly illustrate the invention, the invention is further described below with reference to preferred embodiments and figures. It will be understood by those skilled in the art that the following detailed description is intended to be illustrative and not restrictive, and should not be taken to limit the scope of the invention, which is defined by any of the ranges set forth herein including the endpoints and any number between the endpoints and any sub-range defined by the endpoints or any number between the endpoints.
Example 1
(1) Activating the ganoderma lucidum mycelia: inoculating Ganoderma mycelia on PDA solid culture medium plate, and culturing in 26 deg.C incubator for 7d to make mycelia spread on the surface of the plate;
(2) preparing a potato glucose liquid culture medium: 1% glucose, 0.3% potato extract, 0.2% KH2PO4、0.1％MgSO4﹒7H2O。
(3) Preparing a ganoderma lucidum seed solution: inoculating the activated ganoderma lucidum mycelia with the diameter of 2mm multiplied by 2mm in the step (1) into a potato glucose liquid culture medium, carrying out amplification culture at 26 ℃ at 200r/min, and finishing the culture after the mycelia are agglomerated and hung on the wall to obtain ganoderma lucidum seed liquid, wherein the concentration of the ganoderma lucidum seed liquid is 20 mg/mL;
(4) pretreatment of rice bran raw materials: taking 50g of rice bran, adding 50g of deionized water, uniformly mixing, and sterilizing at high temperature to obtain a rice bran raw material;
(5) solid fermentation: inoculating the seed solution of Ganoderma into rice bran raw material for fermentation culture, wherein the inoculation amount of the seed solution of Ganoderma is 5% of the mass of the rice bran raw material, sealing with a gas permeable membrane, culturing at 26 deg.C for 13 days, vacuum drying at 55 deg.C and-0.1 MPa, and pulverizing to obtain fermented rice bran;
(6) extracting rice bran fermentation crude polysaccharide: adding the fermented rice bran into petroleum ether for extraction, wherein the using amount of the petroleum ether is 1g according to the proportion of the fermented rice bran to the petroleum ether: 5ml, the extraction time is 30min, after suction filtration, the petroleum ether extract is recovered to obtain fermented oil containing rich active substances such as triterpenes, the residual filter residue is added into 75% ethanol solution for extraction, and the dosage of the ethanol solution is 1g according to the proportion of the fermented rice bran to the ethanol solution: 5ml, extracting for 30min, performing suction filtration, wherein a 75% ethanol extract contains nutritional ingredients such as oligosaccharide, short peptide, amino acid and phenols, recovering the extract to prepare a functional beverage, adding the residual filter residue into deionized water for 3 times of water extraction, wherein the water consumption of each time of water extraction is 1g according to the ratio of fermented rice bran to water: 5ml, extracting for 30min at 100 deg.C, and collecting 3 times of water extractive solution to obtain polysaccharide water extractive solution;
adding 2% of amylase and 2% of glucoamylase into the polysaccharide water extract, performing water bath at 55 ℃ for 12h, adding 2% of trypsin, performing water bath at 55 ℃ for 2h, filtering, and taking supernate to obtain enzymolysis water extract; concentrating the enzymolysis water extract, concentrating to obtain enzymolysis water extract concentrated solution with density of 1.3g/mL, adding anhydrous ethanol with volume 4 times of the concentrated solution, storing at 4 deg.C for 12h, centrifuging at 4000r/min for 20min, and collecting precipitate to obtain testa oryzae fermented crude polysaccharide;
(7) obtaining rice bran fermented polysaccharide: purifying the crude rice bran fermented polysaccharide by using DEAE ion exchange resin, wherein the eluent is water, further purifying the eluent by using a gel exclusion chromatographic column, and freeze-drying at the cold trap temperature of-60 ℃ and the vacuum degree of 10Pa to obtain the rice bran fermented polysaccharide, wherein a real object diagram of the rice bran fermented polysaccharide is shown in figure 1 as white powder, and the purity of the rice bran fermented polysaccharide is 99.8% by adopting an area percentage method. FIG. 2 is a graph comparing the total fat, total polysaccharide and total phenol content of the rice bran fermented polysaccharides prepared in example 1 with that of the unfermented rice bran, and it can be seen from FIG. 2 that the total fat and total polysaccharide content of the prepared rice bran fermented polysaccharides is higher than that of the unfermented rice bran, and the total phenol content is reduced.
Test example 1
The rice bran fermented polysaccharides prepared in example 1 were selected for the following tests:
(1) HPGPC measurement: it showed a single peak on HPGPC and a weight average molecular weight of 2.58X 10, as measured by HPGPC4Da；
(2) HPLC-ELSD test: after the rice bran fermented polysaccharide is hydrolyzed, the rice bran fermented polysaccharide is obtained by analyzing with HPLC-ELSD, wherein the weight ratio of L-arabinose: d-mannose: d-glucose was composed in a molar ratio of 0.5:4.7: 5.0.
(3) GC-MS test: is composed ofThe molecular structure of the rice bran fermented polysaccharide is further determined, 10mg of the rice bran fermented polysaccharide reacts with 50mg of sodium hydride under the nitrogen-filled anhydrous condition, then reacts with 10mL of methyl iodide under the same condition to prepare methylated polysaccharide, and the reaction product is analyzed by infrared spectroscopy, and by checking 3200-3600 cm-1Whether there is an absorption peak or not is judged whether there is a residual hydroxyl group. The methylated reaction product was hydrolyzed with 0.1mol/L trifluoroacetic acid at 105 ℃ for 6h, and the hydrolyzate was hydrolyzed with 20mg NaBH4Reduction, followed by reaction with 2mL of acetic anhydride for 2h, to prepare partially methylated acetyl sugar alcohol (PMAA), and analysis of the monosaccharide residues constituting the polysaccharide and their contents using a GC-MS (gas chromatography-Mass Spectrometry) spectrometer are shown in Table 1.
Table 1 GC-MS data obtained for partially methylated acetyl sugar alcohols (PMAA)
(3)600MHz NMR test: the fermented rice bran polysaccharides were analyzed by 600MHz NMR spectrometer after being replaced with heavy water, according to FIG. 31H-NMR analysis shows that the integral ratio of anomeric carbon of D-glucose, L-arabinose and D-mannose is 2.0:0.4:0.9, the D-glucose and the L-arabinose are in alpha configuration, and the D-mannose is in beta configuration;
in FIG. 413Delta 101.5ppm and delta 97.9ppm were determined in C-NMR as anomeric carbon signals;
in FIG. 5, the hydrocarbon signal attribution is determined by HSQC and H-H COSY;
the primary structure of the rice bran fermented polysaccharide obtained by HMBC and NOESY is as follows:
(4) infrared spectrum test: in order to further verify the analysis result of nuclear magnetic resonance, infrared spectrum test is carried out, the obtained result is shown in figure 6, all stretching vibration peaks of the structural group determined by nuclear magnetic resonance are shown in the infrared spectrum, and the structure is confirmed.
(5) Uv-vis spectrophotometry test: after being complexed with Congo red, the rice bran fermented polysaccharide is respectively mixed with NaOH solutions with different concentrations of 0.05g/mL, 0.1g/mL, 0.2g/mL, 0.3g/mL and 0.4g/mL, and the mixture is scanned and analyzed by using a scanning type ultraviolet-visible spectrophotometer, the result is shown in figure 7, and the rice bran fermented polysaccharide molecule has a spiral structure shown in figure 7, wherein the spiral structure is most stable under the NaOH concentration of 0.1 g/mL-0.3 g/mL, the stability is poor along with the increase of the NaOH concentration, and the rice bran fermented polysaccharide is determined to be in a random coil structure through circular dichroism spectrum scanning of figure 8.
Test example 2
10 female tumor-bearing mice with the weight of 20 +/-2.0 g and suffering from H1299 human non-small cell lung cancer are selected, fed under the same environment for 6 days and then randomly divided into two groups, wherein each group comprises 5 female tumor-bearing mice, the control group is orally administered with normal saline every day, the experimental group is prepared by dissolving the rice bran fermented polysaccharide prepared in the example 1 in the normal saline, and the oral administration is performed once every day, and the administration amount is 40 mg/kg. Tumor volumes of nude mice after H1299 cell inoculation were measured weekly, and the results obtained are shown in fig. 9. After 6 weeks of administration, solid tumors were excised to measure the tumor weight, see the real image of the excised tumor body in fig. 10, and the tumor inhibition rate was calculated. The tumor inhibition rate is calculated by the formula: inhibition rate [ (a-B)/a ] x 100%, wherein a is the average weight of the negative control group tumors; b is the average weight of the tumors in the treated group, and the tumor tissue specimens were subjected to pathological examination, the results of which are shown in FIG. 11. During feeding, the rice bran fermented polysaccharide has a remarkable inhibition effect on tumor growth, tumor bodies are picked up after the experiment is finished, the average tumor body mass of an experimental group is weighed to be 0.35 +/-0.11 g, the average tumor body mass of a control group is weighed to be 2.70 +/-0.70 g, the tumor inhibition rate reaches 86.88% +/-3.98%, and the experiment proves that the rice bran fermented polysaccharide effectively inhibits the tumor growth. The HE slice result shows that the experimental group has the phenomenon of tumor cell atrophy and apoptosis.
It should be understood that the above-mentioned embodiments of the present invention are only examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention, and it will be obvious to those skilled in the art that other variations or modifications may be made on the basis of the above description, and all embodiments may not be exhaustive, and all obvious variations or modifications may be included within the scope of the present invention.
Claims (10)
1. The rice bran fermented polysaccharide is characterized by having a structural formula
2. The fermented rice bran polysaccharide of claim 1, wherein the fermented rice bran polysaccharide has a weight average molecular weight of 2.58 x 104Da; wherein the rice bran fermented polysaccharide consists of L-arabinose, D-mannose and D-glucose according to the molar ratio of 0.5:4.7: 5.0.
3. A method for preparing the rice bran fermented polysaccharide according to claim 1 or 2, comprising the steps of:
(1) solid state fermentation: inoculating the ganoderma lucidum seed solution into the rice bran raw material for fermentation culture, drying and crushing the rice bran raw material after the fermentation culture to obtain fermented rice bran;
(2) extracting rice bran fermentation crude polysaccharide: adding fermented rice bran into petroleum ether for extraction, performing suction filtration, adding filter residue into ethanol solution for extraction, performing suction filtration, adding filter residue into water for water extraction to obtain polysaccharide water extract, and performing enzymolysis treatment to obtain enzymolysis water extract; then concentrating the enzymatic water extract to obtain an enzymatic water extract concentrated solution, adding absolute ethyl alcohol, standing, centrifuging, taking precipitate, and drying to obtain rice bran fermented crude polysaccharide;
(3) obtaining rice bran fermented polysaccharide: purifying the crude rice bran fermented polysaccharide by using DEAE ion exchange resin, wherein the eluent is water, further purifying the eluent by using a gel exclusion chromatographic column, and freeze-drying to obtain the rice bran fermented polysaccharide;
wherein the concentration of the ganoderma lucidum seed liquid is 10-20 mg/mL; the inoculation amount of the ganoderma lucidum seed liquid is 2-5% of the mass of the rice bran raw material.
4. The method according to claim 3, wherein the Ganoderma lucidum seed solution is obtained by inoculating activated Ganoderma lucidum mycelia into a liquid medium for scale-up culture; preferably, the activation is carried out for 1-10 days at 25-30 ℃ in a PDA culture medium; preferably, the amplification culture is carried out at 25-30 ℃ and 50-300 r/min.
5. The method according to claim 3, wherein the fermentation culture is carried out at 25 to 30 ℃ for 3 to 15 days.
6. The preparation method according to claim 3, wherein the ratio of the fermented rice bran to the petroleum ether is 1g to 2-6 ml, and the extraction time is 10-40 min; preferably, the ratio of the fermented rice bran to the ethanol solution is 1g to 2-6 ml, and the extraction time is 10-40 min.
7. The preparation method according to claim 3, wherein the ratio of the fermented rice bran to the water is 1g to 2-10 ml, the extraction times are 2-5 times, and the extraction time is 20-40 min.
8. The preparation method according to claim 3, wherein the enzymolysis treatment comprises adding 1-5% of amylase and 1-5% of glucoamylase into an aqueous polysaccharide extract, performing water bath at 40-65 ℃ for 1-12 h, and then adding 1-5% of trypsin, performing water bath at 40-65 ℃ for 1-12 h.
9. The preparation method of claim 3, wherein the volume ratio of the absolute ethyl alcohol to the enzymolysis water extraction concentrated solution is 2-5: 1.
10. Use of the rice bran fermented polysaccharide according to claim 1 or 2 in the preparation of a medicament for the treatment of non-small cell lung cancer.
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| CN1759725A (en) * | 2005-10-25 | 2006-04-19 | 天津科技大学 | Method for producing fermented rice bran through solid-state fermentation method, and application of fermented rice bran |
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| CN108034688A (en) * | 2017-10-31 | 2018-05-15 | 海盐县凌特生物科技有限公司 | The preparation process of rice bran polysaccharide |
| CN109762855A (en) * | 2018-12-15 | 2019-05-17 | 浙江大学 | A kind of new method for defatted rice bran sulphation of fermenting |
| CN112920285A (en) * | 2020-08-13 | 2021-06-08 | 国家粮食和物资储备局科学研究院 | Preparation method and application of rice bran polysaccharide |
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| CN1759725A (en) * | 2005-10-25 | 2006-04-19 | 天津科技大学 | Method for producing fermented rice bran through solid-state fermentation method, and application of fermented rice bran |
| CN105039453A (en) * | 2015-07-20 | 2015-11-11 | 国家粮食局科学研究院 | Method for preparing rice bran polysaccharides with improved oxidation resistance and application of rice bran polysaccharides |
| CN108034688A (en) * | 2017-10-31 | 2018-05-15 | 海盐县凌特生物科技有限公司 | The preparation process of rice bran polysaccharide |
| CN109762855A (en) * | 2018-12-15 | 2019-05-17 | 浙江大学 | A kind of new method for defatted rice bran sulphation of fermenting |
| CN112920285A (en) * | 2020-08-13 | 2021-06-08 | 国家粮食和物资储备局科学研究院 | Preparation method and application of rice bran polysaccharide |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114107409A (en) * | 2021-11-25 | 2022-03-01 | 沈阳市现代农业研发服务中心(沈阳市农业科学院) | Method for processing rice bran meal and product |
| CN114107409B (en) * | 2021-11-25 | 2023-11-17 | 沈阳市农业科学院 | Method for treating rice bran meal and product |
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