CN114100200A - Separation device of platelet-rich plasma and use method thereof - Google Patents
Separation device of platelet-rich plasma and use method thereof Download PDFInfo
- Publication number
- CN114100200A CN114100200A CN202111186985.8A CN202111186985A CN114100200A CN 114100200 A CN114100200 A CN 114100200A CN 202111186985 A CN202111186985 A CN 202111186985A CN 114100200 A CN114100200 A CN 114100200A
- Authority
- CN
- China
- Prior art keywords
- platelet
- rich plasma
- inner cylinder
- separation device
- outer cylinder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000926 separation method Methods 0.000 title claims abstract description 44
- 210000004623 platelet-rich plasma Anatomy 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 10
- 230000007246 mechanism Effects 0.000 claims abstract description 44
- 210000004369 blood Anatomy 0.000 claims abstract description 35
- 239000008280 blood Substances 0.000 claims abstract description 35
- 210000003743 erythrocyte Anatomy 0.000 claims abstract description 21
- 210000002381 plasma Anatomy 0.000 claims description 22
- 239000006228 supernatant Substances 0.000 claims description 14
- 239000003146 anticoagulant agent Substances 0.000 claims description 9
- 229940127219 anticoagulant drug Drugs 0.000 claims description 9
- 230000002457 bidirectional effect Effects 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 5
- 230000002093 peripheral effect Effects 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 9
- 238000005119 centrifugation Methods 0.000 abstract description 4
- 238000002347 injection Methods 0.000 abstract description 4
- 239000007924 injection Substances 0.000 abstract description 4
- 230000007547 defect Effects 0.000 abstract description 2
- 210000000963 osteoblast Anatomy 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 210000004027 cell Anatomy 0.000 description 5
- 230000015572 biosynthetic process Effects 0.000 description 4
- 230000035755 proliferation Effects 0.000 description 4
- 108010073929 Vascular Endothelial Growth Factor A Proteins 0.000 description 3
- 102000005789 Vascular Endothelial Growth Factors Human genes 0.000 description 3
- 108010019530 Vascular Endothelial Growth Factors Proteins 0.000 description 3
- 102000008186 Collagen Human genes 0.000 description 2
- 108010035532 Collagen Proteins 0.000 description 2
- 108050007372 Fibroblast Growth Factor Proteins 0.000 description 2
- 102000018233 Fibroblast Growth Factor Human genes 0.000 description 2
- 108010038512 Platelet-Derived Growth Factor Proteins 0.000 description 2
- 102000010780 Platelet-Derived Growth Factor Human genes 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 230000035605 chemotaxis Effects 0.000 description 2
- 210000001612 chondrocyte Anatomy 0.000 description 2
- 229920001436 collagen Polymers 0.000 description 2
- 229940126864 fibroblast growth factor Drugs 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 208000006386 Bone Resorption Diseases 0.000 description 1
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 1
- 102000004218 Insulin-Like Growth Factor I Human genes 0.000 description 1
- 108010009583 Transforming Growth Factors Proteins 0.000 description 1
- 102000009618 Transforming Growth Factors Human genes 0.000 description 1
- 230000004520 agglutination Effects 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004204 blood vessel Anatomy 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 210000002449 bone cell Anatomy 0.000 description 1
- 210000002805 bone matrix Anatomy 0.000 description 1
- 230000024279 bone resorption Effects 0.000 description 1
- 210000000845 cartilage Anatomy 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 230000002608 insulinlike Effects 0.000 description 1
- 230000003834 intracellular effect Effects 0.000 description 1
- 210000000265 leukocyte Anatomy 0.000 description 1
- 238000000464 low-speed centrifugation Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 230000000399 orthopedic effect Effects 0.000 description 1
- 210000002997 osteoclast Anatomy 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000004033 plastic Substances 0.000 description 1
- 229920003023 plastic Polymers 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000003634 thrombocyte concentrate Substances 0.000 description 1
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
- B01D17/02—Separation of non-miscible liquids
- B01D17/0217—Separation of non-miscible liquids by centrifugal force
Landscapes
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- External Artificial Organs (AREA)
- Centrifugal Separators (AREA)
Abstract
The application discloses a platelet-rich plasma separation device and a use method thereof, wherein a piston mechanism, an inner cylinder and an outer cylinder are coaxially arranged, the inner cylinder and the outer cylinder are hollow cylinders with openings, the piston mechanism is arranged in the inner cylinder, the outer cylinder is sleeved outside the inner cylinder, the inner cylinder is detachably sleeved in the outer cylinder through a first clamping part and a second clamping part and is communicated with the outer cylinder, and two detachable fixed injection cavities are connected to form a unique closed secondary centrifugal device, the platelet-rich plasma separation device has the characteristics of large blood collection amount, large platelet-rich plasma enrichment amount, thorough red blood cell separation and high platelet purity, red blood cells are stored and removed in a fixed proportion space, and the platelet-rich plasma with high concentration is obtained through secondary centrifugation, so that the defects of too complex device structure, small blood collection amount, incomplete red blood cell separation and poor enrichment effect of the existing separation device are overcome, thereby affecting the purity of the platelets.
Description
Technical Field
The application relates to the technical field of medical instruments, in particular to a separation device of platelet-rich plasma and a use method thereof.
Background
Platelets are anucleate cells in mammalian blood, are smaller than erythrocytes and leukocytes, can provide an agglutination effect during hemostasis, and can release a large number of cell growth factors through the degranulation of intracellular a particles, including platelet-derived growth factor (PDGF), transforming growth factor (TGF-P), insulin-like growth factor-l (IGF-l), Fibroblast Growth Factor (FGF), Vascular Endothelial Growth Factor (VEGF) and the like, so that the platelets can induce the division and proliferation and collagen synthesis of bone cells and promote the repair and reconstruction of bone tissues. Wherein, the TOGF can promote chemotaxis and proliferation of osteoblasts and increase the synthesis capacity of collagen; TGF-P has functions of stimulating chemotaxis and proliferation of osteoblast precursor cells and osteoblast, and inhibiting osteoclast formation and bone resorption; IGF-I can increase osteoblast activity and promote generation of cartilage and bone matrix; VEGF can promote the formation of new blood vessels, is beneficial to the supply of nutrient substances in a focal region, and can also directly act on osteoblasts and chondrocytes to enhance the migration, proliferation and cell activity of the osteoblasts and the chondrocytes. The platelets can be extracted from the whole blood of a patient, play a role in treatment after enrichment treatment, have the characteristics of rich sources, convenient material acquisition, simple separation and the like, and are widely applied to the fields of orthopedics, stomatology, sports medicine and the like. The existing manual preparation method of platelets is a Platelet Rich Plasma (PRP) method, and PRP obtained is used for experimental research and clinical treatment.
PRP is a platelet concentrate obtained by centrifuging whole blood, and is prepared by centrifuging blood to separate the blood into layers by utilizing the difference in sedimentation rate of various components in the blood. Whole blood can be divided into three layers by low-speed centrifugation, wherein the upper layer is a platelet poor plasma layer (PPP), the middle layer is a platelet rich plasma layer (PRP), and the lower layer is a red blood cell layer, and highly concentrated platelet-containing plasma can be obtained by taking the middle layer.
The preparation of PRP needs to remove PPP and erythrocyte with separator, and current separator has the device structure too complicated, and blood collection volume is less, erythrocyte separation thoroughly and the not good shortcoming of enrichment effect to influence the purity of platelet.
Disclosure of Invention
The embodiment of the application provides a separation device of rich platelet plasma and a use method thereof, which are used for solving the technical problems that the existing separation device has the defects of too complex structure, small blood collection amount, incomplete red blood cell separation and poor enrichment effect, and the purity of platelets is influenced.
In view of the above, the first aspect of the present application provides a separation device for platelet rich plasma, comprising: the piston mechanism, the inner cylinder and the outer cylinder are coaxially arranged;
the inner cylinder and the outer cylinder are hollow cylinders with openings;
the piston mechanism is arranged in the inner cylinder;
the bottom of the inner barrel is provided with a first clamping part and an inner barrel needle tube connecting part;
the bottom of the outer barrel is provided with an outer barrel needle tube connecting part;
a second clamping part is arranged above the connecting part of the needle tube of the outer barrel;
the inner tube passes through first joint portion with second joint portion detachably cover is established the urceolus is inside and with the urceolus intercommunication.
Optionally, the piston mechanism comprises: the piston sheet, the connecting rod and the holding part;
the piston sheet is arranged at the bottom end of the connecting rod;
the peripheral surface of the piston sheet is abutted against the inner wall of the inner cylinder;
the top end of the connecting rod is fixedly connected with the holding part.
Optionally, the gripping portion is of cylindrical configuration.
Optionally, the piston disc is a rubber piston disc.
Optionally, an opening at the top end of the inner cylinder is symmetrically provided with inner cylinder extension parts protruding outwards;
and the opening at the top end of the outer barrel is symmetrically provided with outer barrel extending parts which protrude outwards.
Optionally, the outer barrel extension and the inner barrel extension are both prism blocks.
Optionally, four corners of the prism block are in a circular arc structure.
Optionally, the inner barrel needle tube connecting part and the outer barrel needle tube connecting part are both downwards hollow and protruding needle hole seats.
Optionally, the first clamping part is a rectangular block;
the second clamping portion is a trapezoidal block protruding upwards.
In a second aspect, the present application provides a method for using a separation device for platelet rich plasma, specifically comprising the following steps:
s1: connecting the outer cylinder needle tube connecting part in the separating device with a blood taking needle, pulling a piston mechanism to extract anticoagulant and blood, taking down the blood taking needle and sealing the outer cylinder needle tube connecting part;
s2: centrifuging the separation device at a low rotating speed, and separating the centrifuged anticoagulant and blood into lower layer red blood cells and upper layer supernatant;
s3: pulling the piston mechanism to pump the supernatant into the inner cylinder, and separating the inner cylinder and the outer cylinder from the residual red blood cells in the outer cylinder;
s4: sealing the connection part of the needle tube of the inner cylinder, centrifuging the inner cylinder at a high rotating speed, separating the centrifuged supernatant into an upper platelet poor plasma layer and a lower platelet rich plasma layer, inserting the bidirectional needle through a piston sheet in the piston mechanism, extruding the piston mechanism to discharge the upper platelet poor plasma layer, and reserving the platelet rich plasma layer.
According to the technical scheme, the embodiment of the application has the following advantages:
the application provides a separator of rich platelet plasma, piston mechanism through coaxial setting, inner tube and urceolus are for having open-ended hollow cylinder, be provided with piston mechanism inside the inner tube, the outer cover of inner tube is equipped with the urceolus, the inner tube overlaps through first joint portion and second joint portion detachably and establishes inside the urceolus and communicate with the urceolus, connect with two fixed injection cavities of dismantling, form unique closed secondary centrifugal device, it is big to have blood collection volume, rich platelet plasma enrichment volume is big, red blood cell separation is thorough and the characteristics that platelet purity is high, with fixed proportion space storage and removal red blood cell, obtain the rich platelet plasma of high concentration through the secondary centrifugation, the shortcoming that current separator has device structure too complicated, blood collection volume is less, red blood cell separation is not thorough and the enrichment effect is not good has been solved, thereby affecting the purity of the platelets.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present application, the drawings needed to be used in the description of the embodiments are briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments described in the present application, and other drawings can be obtained by those skilled in the art according to the drawings.
Fig. 1 is a schematic cross-sectional view of a separation device for platelet rich plasma provided in an embodiment of the present application;
fig. 2 is a schematic perspective view of a separation device for platelet rich plasma provided in an embodiment of the present application;
FIG. 3 is a schematic side view of a platelet rich plasma separation device according to an embodiment of the present disclosure;
fig. 4 is a schematic top view of a platelet rich plasma separation device provided in an embodiment of the present application;
FIG. 5 is a schematic diagram of an inner barrel structure of a separation device for platelet rich plasma provided in an embodiment of the present application;
FIG. 6 is a schematic view of the inner structure of the outer barrel of the separation device for platelet rich plasma provided in the embodiment of the present application;
FIG. 7 is a schematic flow chart of a method for using a separation device for platelet rich plasma according to an embodiment of the present application;
wherein the reference numerals are:
1. an outer cylinder; 11. an outer cylinder needle tube connecting part; 12. a second clamping part; 13. an outer barrel extension; 2. an inner barrel; 21. an inner cylinder needle tube connecting part; 22. an inner cylinder extension; 23. a first clamping part; 3. a piston mechanism; 31. a piston plate; 32. a connecting rod; 33. a grip portion.
Detailed Description
In order to make the technical solutions of the present application better understood, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
Example 1
For ease of understanding, referring to fig. 1 to 6, the present application provides an embodiment of a separation device for platelet rich plasma, comprising: the piston mechanism 3, the inner cylinder 2 and the outer cylinder 1 are coaxially arranged; the inner cylinder 2 and the outer cylinder 1 are hollow cylinders with openings; a piston mechanism 3 is arranged in the inner cylinder 2; the bottom of the inner cylinder 2 is provided with a first clamping part 23 and an inner cylinder needle tube connecting part 21; the bottom of the outer cylinder 1 is provided with an outer cylinder needle tube connecting part 11; a second clamping part 12 is arranged above the needle tube connecting part 11 of the outer barrel; the inner barrel 2 is detachably sleeved inside the outer barrel 1 through the first clamping portion 23 and the second clamping portion 12 and is communicated with the outer barrel 1.
It should be noted that, a piston mechanism 3, an inner cylinder 2 and an outer cylinder 1 are coaxially arranged, the piston mechanism 3, the inner cylinder 2 and the outer cylinder 1 are in a concentric relationship and have a common central longitudinal axis, the inner cylinder 2 and the outer cylinder 1 are not rotatable with each other, the inner cylinder 2 and the outer cylinder 1 are hollow cylinders with openings, the materials of the inner cylinder 2 and the outer cylinder 1 can be selected from plastics or glass, the piston is arranged inside the inner cylinder 2, an inner cylinder needle tube connecting part 21 is arranged inside the inner cylinder 2 near the bottom end opening, first clamping parts 23 are symmetrically arranged at the bottom end outside the inner cylinder 2, a second clamping part 12 is arranged inside the outer cylinder 1, the first clamping part 23 is clamped with the second clamping part 12 to realize the clamping connection of the inner cylinder 2 and the outer cylinder 1, the outer cylinder needle tube connecting part 11 is arranged inside the outer cylinder 1 near the bottom end opening, a placing cavity is formed between the second clamping part 12 inside the outer cylinder 1 and the outer cylinder needle tube connecting part 11, when the separation device works, the bottom of the piston mechanism 3 presses the inner cylinder needle tube connecting part 21, the piston mechanism 3 is pulled to draw blood, the blood enters the placing cavity through the outer cylinder needle tube connecting part 11, the outer cylinder needle tube connecting part 11 is sealed, the whole separation device is centrifuged, the piston mechanism 3 is continuously pulled after centrifugation, at the moment, another placing cavity is formed between the inner cylinder needle tube connecting part 21 and the piston mechanism 3, the centrifuged supernatant is drawn into the cavity between the inner cylinder needle tube connecting part 21 and the piston mechanism 3 through the inner cylinder needle tube connecting part 21, at the moment, red blood cells are left in the outer cylinder 1, then the outer cylinder 1 is separated from the inner cylinder 2, the inner cylinder 2 and the piston mechanism 3 are centrifuged again, the centrifuged supernatant is in a layered state at the moment, a bidirectional needle is inserted through the bottom of the piston mechanism 3 to extrude the piston mechanism 3, the upper platelet poor plasma layer in the stratified fluid is discharged through the bi-directional needle, leaving the desired highly concentrated platelet containing plasma at the bottom of the inner barrel 2.
The application provides a pair of separator of rich platelet plasma, piston mechanism 3 through coaxial setting, inner tube 2 and urceolus 1 are for having open-ended hollow cylinder, 2 inside piston mechanism 3 that are provided with of inner tube, 2 outside covers of inner tube are equipped with urceolus 1, inner tube 2 overlaps through first joint portion 23 and 12 detachably cover of second joint portion and establishes inside and communicate with urceolus 1 of urceolus 1, connect with two fixed injection cavities that can dismantle, form unique closed secondary centrifugal device, it is big to have blood collection volume, rich platelet plasma enrichment volume is big, red cell separation is thorough and the high characteristics of platelet purity, with fixed proportion space storage and get rid of red cell, obtain the rich platelet plasma of high concentration through secondary centrifugation, it is too complicated to have solved current separator to have the device structure, blood collection volume is less, outer tube 1, Incomplete red blood cell separation and poor enrichment effect, thereby affecting the purity of the platelets.
Example 2
For easy understanding, referring to fig. 1, as a further modification of embodiment 1, the piston mechanism 3 includes: the piston plate 31, the connecting rod 32, and the grip 33; the bottom end of the connecting rod 32 is provided with a piston sheet 31; the outer peripheral surface of the piston sheet 31 is abutted against the inner wall of the inner cylinder 2; the top end of the connecting rod 32 is fixedly connected with a holding part 33; the grip 33 is of cylindrical configuration; the piston plate 31 is a rubber piston plate.
It should be noted that, the piston piece 31 is a rubber piston piece, and is low in cost, and the two-way needle of being convenient for pierces through, absorbs the upper anemia platelet plasma layer, and the connecting rod 32 bottom is provided with the piston piece 31, and the piston piece 31 is for dismantling the connection, is convenient for change, only needs to change the piston piece 31 alright continue to use separator, and the piston piece 31 top is provided with the portion of gripping 33, and the portion of gripping 33 is cylindrical structure, also can be for other structures, specifically decides according to people's demand.
Example 3
For easy understanding, please refer to fig. 1 to 6, as a further improvement of embodiment 1, an opening at the top end of the inner cylinder 2 is symmetrically provided with inner cylinder extensions 22 protruding outwards; an opening at the top end of the outer cylinder 1 is symmetrically provided with outer cylinder extending parts 13 which protrude outwards; the outer cylinder extension 13 and the inner cylinder extension 22 are both prism blocks; four corners of the prismatic block are in circular arc structures; the inner cylinder needle tube connecting part 21 and the outer cylinder needle tube connecting part 11 are both downwards hollow and protruding needle hole seats; the first clamping part 23 is a rectangular block; the second catching portion 12 is a trapezoidal block protruding upward.
It should be noted that, urceolus extension 13 and inner tube extension 22 are the arris piece, the symmetry sets up in top opening both sides, hold in order to assist people, urceolus extension 13 sets up with 1 integration of urceolus, inner tube extension 22 sets up with 2 integration of inner tube, four angles of arris piece are the circular arc structure, hinder the hand when avoiding people to separate inner tube 2 and urceolus 1, realize the guard effect, 11 parallel and the downward outstanding pinhole seat of cavity in middle part in urceolus needle tubing connecting portion bottom surface, the downward and the downward outstanding pinhole seat of cavity in middle part in inner tube needle tubing connecting portion 21 bottom surface, downward outstanding pinhole seat both sides are the indent structure, the 12 trapezoidal pieces that upwards outstanding in second joint portion 12 position, 2 outside bottom opening symmetries of inner tube are provided with first joint portion 23, first joint portion 23 cooperation indent structure carries out the joint with second joint portion 12.
Example 4
For easy understanding, referring to fig. 7, the second aspect of the present application provides an embodiment of a method for using a separation device for platelet rich plasma, which specifically includes the following steps:
s1: connecting the outer cylinder needle tube connecting part 11 in the separating device with a blood taking needle, pulling the piston mechanism 3 to draw anticoagulant and blood, taking down the blood taking needle and sealing the outer cylinder needle tube connecting part 11;
s2: centrifuging the separation device at a low rotating speed, and separating the centrifuged anticoagulant and blood into lower layer red blood cells and upper layer supernatant;
s3: pulling the piston mechanism 3 to pump the supernatant into the inner cylinder 2, and separating the inner cylinder 2 and the outer cylinder 1 from the residual red blood cells in the outer cylinder 1;
s4: the inner cylinder needle tube connecting part 21 is sealed, the inner cylinder 2 is centrifuged at high rotating speed, the centrifuged supernatant is divided into an upper platelet poor plasma layer and a lower platelet rich plasma layer, the bidirectional needle is inserted through the piston sheet 31 in the piston mechanism 3, the piston mechanism 3 is extruded to discharge the upper platelet poor plasma layer, and the platelet rich plasma layer is reserved.
It should be noted that, the outer cylinder needle tube connecting part 11 in the separating device is connected with a blood taking needle, at this time, the bottom of the piston mechanism 3 presses the inner cylinder needle tube connecting part 21, the piston mechanism 3 is pulled to draw anticoagulant and blood, the blood is 50ml, a placing cavity is formed between the second clamping part 12 in the outer cylinder 1 and the outer cylinder needle tube connecting part 11, the anticoagulant and the blood enter the placing cavity through the outer cylinder needle tube connecting part 11, the blood taking needle is taken down and the outer cylinder needle tube connecting part 11 is sealed, the whole separating device is centrifuged at low rotation speed, the centrifuged anticoagulant and blood form lower red blood cells and upper supernatant, the piston mechanism 3 is continuously pulled, the centrifuged supernatant is drawn into the cavity between the inner cylinder needle tube connecting part 21 and the piston mechanism 3 through the inner cylinder needle tube connecting part 21, at this time, red blood cells remain in the outer cylinder 1, and then the outer cylinder 1 is separated from the inner cylinder 2, the inner cylinder needle tube connecting part 21 is sealed, the inner cylinder 2 is centrifuged at a high rotating speed, the centrifuged supernatant is divided into an upper platelet poor plasma layer and a lower platelet rich plasma layer, a bidirectional needle is inserted through the piston sheet 31 in the piston mechanism 3, the piston mechanism 3 is extruded to discharge the upper platelet poor plasma layer, the bottom of the inner cylinder 2 is left with required highly concentrated platelet containing plasma, the highly concentrated platelet containing plasma is vibrated and mixed, and the inner cylinder needle tube connecting part 21 is connected with an injection needle to directly inject.
The above embodiments are only used for illustrating the technical solutions of the present application, and not for limiting the same; although the present application has been described in detail with reference to the foregoing embodiments, it should be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions in the embodiments of the present application.
Claims (10)
1. A separation device for platelet rich plasma, comprising: the piston mechanism, the inner cylinder and the outer cylinder are coaxially arranged;
the inner cylinder and the outer cylinder are hollow cylinders with openings;
the piston mechanism is arranged in the inner cylinder;
the bottom of the inner barrel is provided with a first clamping part and an inner barrel needle tube connecting part;
the bottom of the outer barrel is provided with an outer barrel needle tube connecting part;
a second clamping part is arranged above the connecting part of the needle tube of the outer barrel;
the inner tube passes through first joint portion with second joint portion detachably cover is established the urceolus is inside and with the urceolus intercommunication.
2. The platelet rich plasma separation device of claim 1 wherein said piston mechanism comprises: the piston sheet, the connecting rod and the holding part;
the piston sheet is arranged at the bottom end of the connecting rod;
the peripheral surface of the piston sheet is abutted against the inner wall of the inner cylinder;
the top end of the connecting rod is fixedly connected with the holding part.
3. The separation device of platelet rich plasma according to claim 2 wherein said grip portion is of cylindrical configuration.
4. The platelet-rich plasma separation device of claim 2 wherein the piston plate is a rubber piston plate.
5. The separation device for platelet-rich plasma according to claim 1, wherein the opening at the top end of the inner cylinder is symmetrically provided with inner cylinder extensions which protrude outwards;
and the opening at the top end of the outer barrel is symmetrically provided with outer barrel extending parts which protrude outwards.
6. The platelet rich plasma separation device of claim 5 wherein the outer barrel extension and the inner barrel extension are each prism shaped.
7. The separation device for platelet-rich plasma according to claim 6, wherein the four corners of the prism-shaped block are circular arc structures.
8. The separation device for platelet-rich plasma according to claim 1, wherein the inner barrel needle tube connecting part and the outer barrel needle tube connecting part are needle hole seats protruding downwards in a hollow manner.
9. The separation device of platelet-rich plasma according to claim 1, wherein the first clamping portion is a rectangular block;
the second clamping portion is a trapezoidal block protruding upwards.
10. A method for using a platelet-rich plasma separation device, which is implemented based on the platelet-rich plasma separation device according to any one of claims 1 to 9, and specifically comprises the following steps:
s1: connecting the outer cylinder needle tube connecting part in the separating device with a blood taking needle, pulling a piston mechanism to extract anticoagulant and blood, taking down the blood taking needle and sealing the outer cylinder needle tube connecting part;
s2: centrifuging the separation device at a low rotating speed, and separating the centrifuged anticoagulant and blood into lower layer red blood cells and upper layer supernatant;
s3: pulling the piston mechanism to pump the supernatant into the inner cylinder, and separating the inner cylinder and the outer cylinder from the residual red blood cells in the outer cylinder;
s4: sealing the connection part of the needle tube of the inner cylinder, centrifuging the inner cylinder at a high rotating speed, separating the centrifuged supernatant into an upper platelet poor plasma layer and a lower platelet rich plasma layer, inserting the bidirectional needle through a piston sheet in the piston mechanism, extruding the piston mechanism to discharge the upper platelet poor plasma layer, and reserving the platelet rich plasma layer.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111186985.8A CN114100200B (en) | 2021-10-12 | 2021-10-12 | Separation device for platelet-rich plasma and application method of separation device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111186985.8A CN114100200B (en) | 2021-10-12 | 2021-10-12 | Separation device for platelet-rich plasma and application method of separation device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN114100200A true CN114100200A (en) | 2022-03-01 |
| CN114100200B CN114100200B (en) | 2024-09-10 |
Family
ID=80441796
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111186985.8A Active CN114100200B (en) | 2021-10-12 | 2021-10-12 | Separation device for platelet-rich plasma and application method of separation device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN114100200B (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN208183320U (en) * | 2018-03-19 | 2018-12-04 | 哈密绿天使纤维科技有限公司 | Dewaterer and cotton processing equipment |
| CN113456910A (en) * | 2021-08-12 | 2021-10-01 | 起源细胞技术(滁州)有限公司 | Platelet-rich plasma collecting and separating device adopting physical (freezing) anticoagulation |
| CN218686569U (en) * | 2021-10-12 | 2023-03-24 | 海南苏生生物科技有限公司 | Separation device of platelet-rich plasma |
-
2021
- 2021-10-12 CN CN202111186985.8A patent/CN114100200B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN208183320U (en) * | 2018-03-19 | 2018-12-04 | 哈密绿天使纤维科技有限公司 | Dewaterer and cotton processing equipment |
| CN113456910A (en) * | 2021-08-12 | 2021-10-01 | 起源细胞技术(滁州)有限公司 | Platelet-rich plasma collecting and separating device adopting physical (freezing) anticoagulation |
| CN218686569U (en) * | 2021-10-12 | 2023-03-24 | 海南苏生生物科技有限公司 | Separation device of platelet-rich plasma |
Also Published As
| Publication number | Publication date |
|---|---|
| CN114100200B (en) | 2024-09-10 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN218686569U (en) | Separation device of platelet-rich plasma | |
| US10393728B2 (en) | Apparatus and method for separating and concentrating fluids containing multiple components | |
| US8802362B2 (en) | Methods and devices for separating liquid components | |
| US20080190857A1 (en) | System and Methods of Producing Membranes | |
| US20040071786A1 (en) | Methods and devices for separating liquid components | |
| CN104039372B (en) | Blood constitutent separator | |
| JP4983204B2 (en) | Centrifuge container and centrifuge method | |
| US20090258056A1 (en) | Systems and methods for preparing autologous fibrin glue | |
| CN115501923B (en) | Centrifugal separation tube and use method and application thereof | |
| CN108654141A (en) | A kind of platelet rich plasma preparation facilities | |
| US12023425B2 (en) | Connector for creating platelet rich plasma | |
| JP2012006937A (en) | Method and device for separating liquid component | |
| CN105498293B (en) | A kind of floated platelet rich plasma separating extraction device of buoy | |
| CN114100200A (en) | Separation device of platelet-rich plasma and use method thereof | |
| TWI736824B (en) | Blood separation method | |
| WO2007021344A1 (en) | Systems and methods for preparing autologous fibrin glue | |
| TWM483797U (en) | Device for separating blood components | |
| CN214717523U (en) | Plasma separating tube | |
| CN209361916U (en) | Platelet rich plasma preparation facilities | |
| CN112237755A (en) | Preparation method and preparation device of platelet rich plasma and prepared platelet rich plasma | |
| CN218106152U (en) | Disposable assembled active bone graft preparation device | |
| CN220812461U (en) | Apparatus for preparing autologous thrombin | |
| CN213255118U (en) | Platelet-rich plasma separation device | |
| CN216703068U (en) | Combined and detachable platelet-rich plasma preparation device | |
| CN218554409U (en) | Blood PRP separator |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |