CN214717523U - Plasma separating tube - Google Patents

Plasma separating tube Download PDF

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Publication number
CN214717523U
CN214717523U CN202120198167.9U CN202120198167U CN214717523U CN 214717523 U CN214717523 U CN 214717523U CN 202120198167 U CN202120198167 U CN 202120198167U CN 214717523 U CN214717523 U CN 214717523U
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cap
tube
pipe
plasma separation
plasma
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CN202120198167.9U
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吴道贫
覃绍君
赖小华
邹洁宜
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Guangzhou Zhunyou Biological Technology Co ltd
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Guangzhou Zhunyou Biological Technology Co ltd
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Abstract

The utility model relates to a plasma separation tube, including body, first pipe cap and second pipe cap, the both ends of body have the mouth of pipe, first pipe cap with the second pipe cap respectively with thereby the connection can be dismantled and seal the mouth of pipe, the intussuseption of first pipe cap is filled with blood separation and glues. Can accomplish twice centrifugation through this plasma separator tube in same centrifuge tube, can directly obtain the high-purity platelet plasma of effectively having got rid of erythrocyte and leucocyte under the condition of not changing centrifugal container, the operation is more convenient, has alleviateed operating personnel work load, has also avoided the pollution that many times the transfer caused.

Description

Plasma separating tube
Technical Field
The utility model relates to the technical field of medical equipment, especially relate to a plasma separation tube.
Background
Platelet-rich plasma (PRP) is plasma containing a high volume fraction of platelets obtained by centrifugation of whole blood of a human or animal per se. In addition to the agglutination, human platelets contain many growth factors related to wound healing and bone regeneration, such as platelet-derived growth factor (PDGF), Transforming Growth Factor (TGF) - β 1, 2, insulin-like growth factor (IGF), Epidermal Growth Factor (EGF), and Vascular Endothelial Growth Factor (VEGF). The concentrated plasma contains high-quality fraction of growth factors and plays an important role in local tissue repair and regeneration. The primary mechanism by which PRP promotes bone regeneration is that it releases some growth factors for the local microenvironment of the bone defect, with the growth factors that play a major role being PDGF and TGF- β present in the α -granule. TGF- β in the alpha granule is mostly present in its precursor form, which, when locally injured in the body, is converted into active transforming growth factor β via the exocytosis pathway and activated by some relevant enzymes. When catalysts such as thrombin and calcium chloride are added into PRP, the growth factors in the platelets are activated and released, thereby promoting the repair and regeneration of various tissues. According to research, when PRP is not activated, the amount of growth factors released by alpha granules in platelets is small, the release amount of the growth factors is greatly increased after PRP is activated, and the proliferation promoting effect of the activated PRP is obviously stronger than that of the inactivated PRP. And with the increase of thrombin concentration, the amount of growth factors released by PRP is increased.
PRP has been gradually applied to tissue repair in the fields of oral cavity, plastic surgery, orthopedics, otorhinolaryngology, neurosurgery, and the like. For example, the repair of bone defects is one of the difficulties faced by clinical orthopedics, and the current methods for repairing bone defects mainly include autologous bone grafting, allogeneic bone grafting, biomaterial filling, tissue engineering technology, gene therapy and the like. Although satisfactory curative effect can be achieved during autologous bone grafting, the bone source is limited, and the bone taking not only needs additional operation, but also increases pain of patients, and simultaneously causes various postoperative complications and additional injuries.
The preparation method of PRP has not formed a uniform standard, and the preparation method mainly comprises 2 methods of density gradient centrifugation and plasma separation and replacement. The density gradient centrifugation method is to separate and extract PRP from whole blood according to the different sedimentation coefficients of each component in the blood. The separation technology commonly used at present is mainly a centrifugal separation method, namely a product is obtained through 2-3 times of centrifugation, and the defects are that a cell layering interface is not obvious, platelets cannot be accurately separated through naked eye judgment, and the product can be mixed with a small amount of red blood cells or white blood cells. While platelet-rich plasma with different composition is widely used, for example, platelet-rich plasma containing leukocytes is not suitable for bone joint treatment, so that platelet-rich plasma containing even a small amount of contaminating cells is not acceptable. Moreover, the multiple separation using a common separation tube requires the use of a plurality of separation tubes, and the transfer of plasma between the plurality of separation tubes is cumbersome.
SUMMERY OF THE UTILITY MODEL
Therefore, there is a need for a plasma separation tube with good separation effect and convenient operation.
The utility model provides a plasma separation tube, includes body, first pipe cap and second pipe cap, the both ends of body have the mouth of pipe, first pipe cap with the second pipe cap respectively with thereby the connection can be dismantled at the both ends of body seals the mouth of pipe, first pipe cap intussuseption is filled with blood separation and glues.
The utility model discloses a plasma separator tube can choose to pour into body into blood through second pipe cap one end when using, then carries out centrifugation for the first time with plasma separator tube insert the centrifuge adapter under first pipe cap one end state down. Because the first tube cap is filled with the blood separating gel, the first tube cap is characterized in that the first tube cap can become viscous liquid under the action of high centrifugal force, so that red blood cells, white blood cells and the like can enter, and the first tube cap can become solid when the centrifugation is stopped, so that the red blood cells and the white blood cells in the blood can be settled into the blood separating gel due to high specific gravity after the centrifugation is finished, and the blood separating gel is fixed along with the coagulation of the blood separating gel. Then, the plasma separation tube is turned over to enable one end of the first tube cap to face upwards, the first tube cap is taken down, the plasma separation tube is inserted into the centrifuge adapter in a state that one end of the second tube cap faces downwards for secondary centrifugation, and after the centrifugation is finished, high-purity platelet-rich plasma can be obtained from the tube bottom at one end of the second tube cap. So, can accomplish 2 times centrifugation in same centrifuge tube through this plasma separation tube, can directly obtain the high-purity platelet plasma who effectively has got rid of erythrocyte and leucocyte under the condition of not changing centrifugal container, the operation is more convenient, has alleviateed operating personnel work load, has also avoided the pollution that many times the transfer caused.
In one embodiment, the second cap is a rubber cap.
In one embodiment, the second cap is a butyl rubber cap.
In one embodiment, the cavity formed by the inner wall of the second pipe cap is conical.
In one embodiment, the inner wall of the tube is provided with a blood anticoagulant coating.
In one embodiment, the tube body is cylindrical, the second cap has a cylindrical insertion portion, and the radius of the insertion portion is smaller than that of the tube body, and the insertion portion can be inserted into the tube body so as to detachably connect the second cap with the tube body.
In one embodiment, the device further comprises a spare pipe cap, wherein the spare pipe cap can be detachably connected with one end of the pipe body instead of the first pipe cap so as to close the nozzle.
In one embodiment, the first pipe cap and the spare pipe cap are provided with external threads, and one end of the pipe body is provided with internal threads matched with the external threads.
In one embodiment, the outer wall of the spare pipe cap is also provided with concave-convex textures.
In one embodiment, the outer wall of the tube body is provided with scale marks.
Drawings
FIG. 1 is a schematic diagram of a plasma separation tube according to an embodiment;
FIG. 2 is a side view of the plasma separation tube shown in FIG. 1;
FIG. 3 is an exploded view of the plasma separation tube shown in FIG. 1;
fig. 4 is a schematic structural view of a spare cap of the plasma separation tube according to an embodiment.
Detailed Description
In order to make the above objects, features and advantages of the present invention more comprehensible, embodiments of the present invention are described in detail below with reference to the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, as those skilled in the art will be able to make similar modifications without departing from the spirit and scope of the present invention.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", and the like, indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention. Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise. In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art. In the present application, unless expressly stated or limited otherwise, the first feature may be directly on or directly under the second feature or indirectly via intermediate members. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature. It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein are for illustrative purposes only and do not denote a unique embodiment.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
As shown in fig. 1 to 3, the plasma separation tube 100 according to an embodiment of the present invention includes a tube body 10, a first tube cap 20 and a second tube cap 30, both ends of the tube body 10 have tube openings, the first tube cap 20 and the second tube cap 30 are detachably connected to both ends of the tube body 10 respectively to seal the tube openings, and the first tube cap 20 is filled with blood separation gel.
The utility model discloses a plasma separation tube 100 can choose to inject blood into body 10 through second tube cap 30 one end when using, then insert plasma separation tube 100 and carry out centrifugation for the first time in the centrifuge adapter under the state that first tube cap 20 one end is down. Since the first cap 20 is filled with the blood separation gel, it is characterized in that it becomes a viscous liquid under the action of high centrifugal force, so that red blood cells and white blood cells can enter, and becomes a solid when the centrifugation is stopped, so that the red blood cells and white blood cells in the blood can settle into the blood separation gel due to the high specific gravity after the centrifugation is finished, and can be fixed along with the coagulation of the blood separation gel. Then, the plasma separation tube 100 is turned over so that one end of the first tube cap 20 faces upward, the first tube cap 20 is removed, the plasma separation tube 100 is inserted into the centrifuge adapter in a state that one end of the second tube cap 30 faces downward for second centrifugation, and after the centrifugation is finished, high-purity platelet-rich plasma can be obtained from the tube bottom at one end of the second tube cap 30. So, can accomplish 2 times centrifugation in same centrifuge tube through this plasma separation tube 100, can directly obtain the high-purity platelet plasma of effectively having got rid of erythrocyte and leucocyte under the condition of not changing centrifugal container, the operation is more convenient, has alleviateed operating personnel work load, has also avoided the pollution that many times the transfer caused.
In one particular example, the second cap 30 is a rubber cap. So, can directly pass second tube cap 30 through the syringe before centrifugation for the first time in order to pour into the body 10 with blood into, moreover after centrifugation for the second time, also can directly pass second tube cap 30 through the syringe and conveniently absorb high-purity platelet plasma with the tube end from second tube cap 30 one end to further simplify the operation, reduced the pollution risk.
In one particular example, the second cap 30 is a butyl rubber cap. The butyl rubber has good air tightness, heat resistance, ageing resistance, chemical resistance, shock absorption, electric insulation performance and good resistance to sunlight and ozone, and can be exposed to animal or vegetable oil or oxidizable chemicals, so that the butyl rubber pipe cap is more reliable.
In one specific example, the cavity formed by the inner wall of the second cap 30 is tapered. So, after the centrifugation of second time finishes, can follow the toper bottom of second tube cap 30 and draw high-purity platelet plasma more conveniently, further reduce the operation degree of difficulty. Optionally, the tapered necking direction is a direction away from the opening of the second cap 30, and the inner diameter of the cavity is gradually reduced from the opening of the second cap 30 to the bottom.
In one embodiment, the interior walls of the tube 10 are provided with a blood anticoagulant coating to help remove or inhibit certain coagulation factors from the blood during centrifugation, preventing blood coagulation, and thereby enhancing the centrifugation.
In a specific example, the tube 10 is cylindrical, the second cap 30 has a cylindrical socket 31, and the radius of the socket 31 is smaller than that of the tube 10, and the socket 31 can be inserted into the tube 10 to detachably connect the second cap 30 to the tube 10. It is understood that the shape of the pipe body 10 is not limited thereto, and the connection manner of the second cap 30 and the pipe body 10 is also not limited thereto, and may be adjusted as needed.
Optionally, as shown in fig. 4, the plasma separation tube 100 further includes a spare cap 40, and the spare cap 40 can be detachably coupled to one end of the tube body 10 instead of the first cap 20 to close the tube opening. In this manner, the first cap 20 can be removed and replaced with a spare cap 40 to close the orifice prior to the second centrifugation, thereby reducing the risk of contamination.
Alternatively, the first cap 20 and the spare cap 40 are each provided with an external thread, and one end of the pipe body 10 is provided with an internal thread for cooperating with the external thread, so that the first cap 20 and the spare cap 40 can be threadedly connected with the pipe body 10. It is to be understood that the connection manner of the first cap 20 and the spare cap 40 to the pipe body 10 is not limited thereto, and may be adjusted as needed. In one embodiment, the outer wall of the spare cap 40 is also textured to increase friction for rotational operation. Specifically, the asperity pattern is formed by a plurality of stripe-shaped projections arranged in parallel at intervals.
In one embodiment, the outer wall of the tube 10 is provided with graduations and the tube 10 is transparent so that the volume of liquid in the tube can be easily observed. Optionally, the tube body 10, the first cap 20 and the spare cap 40 are made of PET. Optionally, the ends of first cap 20 and second cap 30 are spherical, but are not limited thereto, and may be flat, for example. Alternatively, the specific gravity of the plasma separation gel is 1.01g/mL to 1.08g/mL, such as 1.05 g/mL. Alternatively, the plasma separation tube 100 may be internally evacuated prior to use, which may reduce the risk of contamination.
The utility model discloses a plasma separator tube 100 is when using, and the accessible syringe passes second tube cap 30 and pours blood into body 10 into, then makes a round trip to overturn plasma separator tube 100, makes the blood anticoagulant mixing on blood and the inner wall, carries out the low-speed centrifugation for the first time in inserting the centrifuge adapter with plasma separator tube 100 under the state that first tube cap 20 one end is down. Since the first cap 20 is filled with the blood separation gel, it is characterized in that it becomes a viscous liquid under the action of high centrifugal force, so that red blood cells and white blood cells can enter, and becomes a solid when the centrifugation is stopped, so that the red blood cells and white blood cells in the blood can settle into the blood separation gel due to the high specific gravity after the centrifugation is finished, and can be fixed along with the coagulation of the blood separation gel. Then, the plasma separation tube 100 is turned over to make one end of the first tube cap 20 face upwards, the first tube cap 20 is taken down, the spare tube cap 40 is replaced, the plasma separation tube 100 is inserted into the centrifuge adapter in a state that one end of the second tube cap 30 faces downwards to perform secondary high-speed centrifugation, and after the centrifugation is finished, the injector is inserted into the conical cavity of the second tube cap 30, so that the high-purity platelet-rich plasma can be sucked. So, can accomplish 2 times centrifugation in same centrifuge tube through this plasma separation tube 100, can directly obtain the high-purity platelet plasma of effectively having got rid of erythrocyte and leucocyte under the condition of not changing centrifugal container, the operation is more convenient, has alleviateed operating personnel work load, has also avoided the pollution that many times the transfer caused.
The technical features of the embodiments described above may be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the embodiments described above are not described, but should be considered as being within the scope of the present specification as long as there is no contradiction between the combinations of the technical features.
The above-mentioned embodiments only represent some embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.

Claims (10)

1. The utility model provides a plasma separation tube, its characterized in that, includes body, first pipe cap and second pipe cap, the both ends of body have the mouth of pipe, first pipe cap with the second pipe cap respectively with the both ends of body can be dismantled and be connected thereby seal the mouth of pipe, first pipe cap intussuseption is filled with blood separation and glues.
2. The plasma separation tube of claim 1, wherein the second tube cap is a rubber tube cap.
3. The plasma separation tube of claim 2, wherein the second cap is a butyl rubber cap.
4. The plasma separation tube of claim 1, wherein the cavity formed by the inner wall of the second tube cap is tapered.
5. The plasma separation tube of claim 1, wherein an inner wall of the tube body is provided with a blood anticoagulant coating.
6. The plasma separation tube according to any one of claims 1 to 5, wherein the tube body is cylindrical, the second cap has a cylindrical insertion portion having a smaller radius than the tube body, and the insertion portion is insertable into the tube body so as to detachably connect the second cap to the tube body.
7. The plasma separation tube of claim 6, further comprising a backup cap removably connectable to one end of the tube body in place of the first cap to close the nozzle.
8. The plasma separation tube of claim 7, wherein the first cap and the spare cap are each provided with an external thread, and one end of the tube body is provided with an internal thread for mating with the external thread.
9. The plasma separation tube of claim 8, wherein the outer wall of the spare cap is further provided with a concave-convex texture.
10. The plasma separation tube according to any one of claims 1 to 5, wherein the outer wall of the tube body is provided with graduation marks.
CN202120198167.9U 2021-01-25 2021-01-25 Plasma separating tube Active CN214717523U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202120198167.9U CN214717523U (en) 2021-01-25 2021-01-25 Plasma separating tube

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202120198167.9U CN214717523U (en) 2021-01-25 2021-01-25 Plasma separating tube

Publications (1)

Publication Number Publication Date
CN214717523U true CN214717523U (en) 2021-11-16

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Application Number Title Priority Date Filing Date
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Country Status (1)

Country Link
CN (1) CN214717523U (en)

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