CN111558474A

CN111558474A - Combined disposable PRP preparation syringe and PRP preparation method

Info

Publication number: CN111558474A
Application number: CN202010287577.0A
Authority: CN
Inventors: 郭燕庆; 孙殿珉
Original assignee: Individual
Current assignee: Individual
Priority date: 2020-04-13
Filing date: 2020-04-13
Publication date: 2020-08-21

Abstract

The present disclosure provides a combination type disposable PRP preparation syringe and a PRP preparation method, the syringe comprising: the first pipe section is cylindrical, and the top end of the first pipe section is of an open structure; the side wall of the second pipe body section is in arc-shaped adduction, the cross-sectional area of the second pipe body section is gradually reduced from the position close to the first pipe body section to the position far away from the first pipe body section, and the bottom end of the second pipe body section is of an open structure; the sealing head is arranged in the first pipe body section, divides the first pipe body section into an upper chamber and a lower chamber, and can move up and down in the first pipe body section; the first luer connector is arranged at the bottom end opening of the second pipe body section. The blood purification device can avoid pollution caused by the fact that pollutants such as external dust and bacteria are mixed into blood, and meanwhile can accurately remove red blood cells in the blood.

Description

Combined disposable PRP preparation syringe and PRP preparation method

Technical Field

Embodiments of the present disclosure relate generally to the technical field of medical devices, and more particularly, to a set-up single use PRP preparation syringe and a PRP preparation method.

Background

Platelet Rich Plasma (PRP), which is a fraction of platelet rich plasma obtained by centrifugation, has been used in many fields such as plastic surgery, sports medicine, orthopedics, neurosurgery, and the like. The main technical means who draws PRP at present adopts centrifugal mode, main instrument consumptive material is centrifuge and centrifuging tube, need use the syringe to input plasma into the centrifuging tube and take out plasma after the centrifugation is accomplished when using again, the operation is comparatively loaded down with trivial details, twice suction can destroy the cell layering in the preparation process, lead to the PRP that can not accurate preparation required concentration, can not keep or get rid of the erythrocyte as required, and pollutant such as dust and bacterium easily gets into the centrifuging tube in operation process, thereby cause blood pollution.

Disclosure of Invention

In a first aspect of the present disclosure, there is provided a set-up single use PRP preparation syringe comprising: the first pipe section is cylindrical, and the top end of the first pipe section is of an open structure; the side wall of the second pipe body section is in arc-shaped adduction, the cross-sectional area of the second pipe body section is gradually reduced from the position close to the first pipe body section to the position far away from the first pipe body section, and the bottom end of the second pipe body section is of an open structure; the sealing head is arranged in the first pipe body section, divides the first pipe body section into an upper chamber and a lower chamber, and can move up and down in the first pipe body section; the first luer connector is arranged at the bottom end opening of the second pipe body section.

The above aspects and any possible implementations further provide an implementation where a push-pull rod is removably attached to the seal head, the push-pull rod capable of pushing and pulling the seal head to move the seal head up and down within the first pipe section.

The above aspect and any possible implementation manner further provide an implementation manner, in which the push-pull rod includes a rod portion and a connecting portion, the sealing head is provided with a second luer connector, and the connecting portion is connected to the second luer connector.

In accordance with the above aspect and any possible implementation manner, there is further provided an implementation manner, wherein an axial distance from an arc vertex of the arc-shaped sidewall of the second pipe body segment to a bottom end of the second pipe body segment is 4mm, and a scale is marked on the second pipe body segment at a position which is 2mm, 4mm and/or 6mm from the bottom end of the second pipe body segment.

The above aspect and any possible implementation further provide an implementation in which the second tube section has a height of 8 mm.

The above aspect and any possible implementation manner further provide an implementation manner, wherein a flange is arranged on the periphery of the upper end of the first pipe body section, and an annular handle is sleeved on the flange.

The above aspect and any possible implementation further provide an implementation in which the inner wall of the first tube section and/or the inner wall of the second tube section is mirror-ground to have a surface roughness of 0.01 μm or less.

The aspect and any possible implementation described above, further providing an implementation in which the first tube section has a height of 118mm and an inner diameter of 28 mm; the first pipe body section is marked with scales and a volume.

The above-described aspects and any possible implementation further provide an implementation, further including: one or more of a blood taking needle, a plug, an L-shaped transparent double-way connector and a red removing needle are used for being connected to the luer connector.

In a second aspect of the present disclosure, there is provided a PRP preparation method, comprising: drawing the desired blood using the configured single use PRP preparation syringe according to any one of the first aspects; placing the assembled disposable PRP preparation syringe into a centrifuge for first centrifugation treatment, and discharging red blood cells or most of red blood cells in blood; and (4) carrying out second centrifugation treatment on the residual blood after the first centrifugation treatment to obtain the required PRP.

In the assembled disposable PRP syringe and the PRP preparation method provided by the embodiment of the disclosure, a first tube body section and a second tube body section which are sequentially connected are adopted, a sealing head which can move up and down in the first tube body section is arranged in the first tube body section, blood is sucked into the syringe by utilizing an opening structure at the bottom end of the second tube body section through the up-and-down movement of the sealing head, and the syringe is sealed by a first Ruhr joint for subsequent centrifugal treatment, and plasma is treated in a closed environment in the whole process, so that pollution caused by the fact that pollutants such as external dust and bacteria are mixed into the plasma is avoided. Simultaneously, the lateral wall of second body section is the arc adduction and the cross-sectional area of second body section reduces from being close to first body section to keeping away from first body section gradually, this kind of "receipts waist formula" structure can be with the high rising of the red blood cell of equal volume wherein than "toper" structure, can produce an obvious light refraction under the illumination of light such as sunshine or light simultaneously, thereby can observe the position of red blood cell more clearly, be favorable to reminding the preparation personnel to avoid excessively going red (excessively going red can lead to the platelet to get rid of excessively, thereby make the PRP concentration of preparing reduce, influence the repair of bone and cartilage), and then get rid of red blood cell more accurately.

It should be understood that what is described in this disclosure section is not intended to limit key or critical features of the embodiments of the disclosure, nor is it intended to limit the scope of the disclosure. Other features of the present disclosure will become apparent from the following description.

Drawings

The above and other features, advantages and aspects of various embodiments of the present disclosure will become more apparent by referring to the following detailed description when taken in conjunction with the accompanying drawings. In the drawings, like or similar reference characters designate like or similar elements, and wherein:

fig. 1 shows a schematic structural view of a modular single use PRP preparation syringe of an embodiment of the present disclosure;

fig. 2 shows a schematic structural view of a second tube section of a ganged single use PRP preparation syringe of an embodiment of the present disclosure;

fig. 3 shows a schematic structural view of a push-pull rod of a modular single use PRP preparation syringe of an embodiment of the present disclosure;

fig. 4 illustrates a top view of a sealing head of a modular single use PRP preparation syringe of an embodiment of the present disclosure;

fig. 5 shows a schematic view of a use state of a assembled single use PRP preparation syringe of an embodiment of the present disclosure;

fig. 6 shows a schematic structure of a de-reddening needle of a set-up single use PRP preparation syringe according to an embodiment of the present disclosure.

Wherein,

10. a first pipe section; 11. a sealing head; 111. a second luer fitting; 12. a push-pull rod; 121. a rod portion; 122. a connecting portion; 13. a flange;

20. a second pipe section;

30. a first luer fitting; 31. a blood collection needle; 32. a plug; 33. an L-shaped transparent double-way joint; 34. removing red needles; 341. a connecting end; 342. a pipe body; 343. a bar-shaped handle.

Detailed Description

To make the objects, technical solutions and advantages of the embodiments of the present disclosure more clear, the technical solutions of the embodiments of the present disclosure will be described clearly and completely with reference to the drawings in the embodiments of the present disclosure, and it is obvious that the described embodiments are some, but not all embodiments of the present disclosure. All other embodiments, which can be derived by a person skilled in the art from the embodiments disclosed herein without making any creative effort, shall fall within the protection scope of the present disclosure.

In addition, the term "and/or" herein is only one kind of association relationship describing an associated object, and means that there may be three kinds of relationships, for example, a and/or B, which may mean: a exists alone, A and B exist simultaneously, and B exists alone. In addition, the character "/" herein generally indicates that the former and latter related objects are in an "or" relationship.

Referring to fig. 1, the assemblable single-use PRP preparation syringe includes a first tube section 10 and a second tube section 20 connected in sequence from top to bottom. The assembled single-use PRP syringe may be formed by integrally molding the first and

second tube segments

10 and 20, or by connecting the first and

second tube segments

10 and 20.

The first pipe section 10 is cylindrical and has an open top end, a sealing head 11 is disposed inside the first pipe section 10, the sealing head 11 divides the first pipe section 10 into an upper chamber and a lower chamber, and the sealing head 11 can move up and down inside the first pipe section 10, so as to change the capacity of the upper chamber and the lower chamber. The sealing head 11 can fill the lower chamber with blood, and reduce the fluctuation of the liquid level of the blood in the centrifugal process, thereby improving the centrifugal efficiency.

For example, the sealing head 11 may be made of rubber, and the sealing head 11 is cylindrical and laterally clamped in the first pipe section 10 to divide the first pipe section 10 into an upper chamber and a lower chamber.

To achieve the up and down movement of the sealing head 11 within the first pipe section 10, a push-pull rod 12 may be detachably connected to the sealing head 11, and the push-pull rod 12 pushes and pulls the sealing head 11 up and down within the first pipe section 10.

Specifically, referring to fig. 3, the push-pull rod 12 includes a rod portion 121 and a connecting portion 122 connected to each other. In order to facilitate the pushing and pulling of the push-pull rod 12, a disk-shaped push-pull portion is disposed at the top end of the rod portion 121, and protrusions distributed along the circumferential direction of the rod portion are disposed on the outer surface of the connecting portion 122, or threads are disposed on the outer surface of the connecting portion 122. Referring also to fig. 4, a second luer 111 is provided on the upper surface of the sealing head 11, for example, the second luer 111 may be provided at the center of the upper surface of the sealing head 11.

When the push-pull rod 12 is connected to the sealing head 11, the connecting portion 122 may be inserted into the second luer 111, and a protrusion on the connecting portion 122 may be engaged with the second luer 111, or a thread on the connecting portion 122 may be screwed into the second luer 111. For example, the connecting portion 122 can be screwed to the second luer 111, and since the force required for the push-pull rod 12 to push and pull the sealing head 11 is small, the above-mentioned connecting manner can ensure that the push-pull rod 12 does not fall off from the sealing head 11 when the push-pull rod 12 pushes and pulls the sealing head 11, and also facilitates the detachment of the push-pull rod 12.

To facilitate the pushing and pulling of the push-pull rod 12, a flange 13 may be provided at the periphery of the upper end of the first pipe section 10, the flange 13 being capable of acting as a support force point when the operator operates the push-pull rod 12. Furthermore, an annular handle can be sleeved outside the flange 13, so that the contact area of an operator can be increased when the push-pull rod 12 is pushed and pulled, and the force can be used more effectively.

Since the top end of the first tube section 10 is an open structure, the upper chamber inside the first tube section 10 is in communication with the outside, and the lower chamber is used for storing the inhaled blood. To clearly show the amount of blood drawn into the lower chamber, the first tube section 10 may be marked with a scale and volume to facilitate viewing of the amount of blood within the first tube section 10.

The first pipe section 10 has different specifications, i.e. different heights and/or diameters of the cross-section, for different uses. For example, for bone and cartilage repair, the first tube section 10 may be 118mm in height and 28mm in inner diameter; for cosmetic and soft tissue repair purposes, the first tube section 10 may be 100mm in height and 28mm in internal diameter.

The side wall of the second tube body section 20 is in arc-shaped inward contraction, the cross-sectional area of the second tube body section 20 is gradually reduced from the position close to the first tube body section 10 to the position far away from the first tube body section 10, namely, the side wall of the second tube body section 20 is in an arc-shaped structure, and the arc vertex of the second tube body section 20 faces the axis of the second tube body section 20, the height of red blood cells with the same volume in the second tube body section can be increased by the waist-shaped structure compared with that in a cone-shaped structure, and meanwhile, under the irradiation of rays such as sunlight or light, obvious light refraction can be generated, so that the position of the red blood cells can be observed more clearly, and the method is favorable for reminding a preparation person to avoid excessive red blood removal (the excessive red blood removal can cause excessive removal of platelets, so that the prepared PRP concentration is reduced, and the.

The bottom end of the second pipe body section 20 is of an open structure, and a first luer 30 is arranged at the opening, so that different accessories can be connected according to different use scenes, and the universality of the injector is enhanced. The first luer fitting 30 may be opened or closed by a stopper.

Referring to fig. 2, the height of the second tube section 20 may be 8 mm. The axial distance H of the arc apex B of the arc-shaped sidewall of the second tube section 20 from the bottom end D of the second tube section 20 can be 4mm, and the axial distance from the bottom end D of the second tube section 20 is marked with scales and volumes at 2mm, 4mm and/or 6mm, so as to facilitate observation of the amount of blood located in the second tube section 20.

The inner walls of the first tube section 10 and/or the second tube section 20 can be mirror-ground to make the inner walls as smooth as possible, for example, the surface roughness of the inner walls can be less than or equal to 0.01 micrometer, so that blood cells can be protected during the centrifugal preparation process, the damage to platelets can be reduced, the integrity of the platelets can be ensured as much as possible, and the activity of the platelets to release growth factors after PRP activation can be improved.

Referring to fig. 5, when blood is to be collected, a blood collection needle 31 is connected to the luer connector 30, blood is collected to a required amount by pulling the push-pull rod 12, then the push-pull rod 12 is removed from the sealing head 11 and the blood collection needle 31 is removed from the first luer connector 30, the luer connector 30 is sealed by using a plug 32, and the syringe tube is put into a special horizontal rotor large-radius low-speed centrifuge (centrifugal radius 18cm, rotation speed 2000r/min) to be subjected to a first centrifugation treatment (centrifugation time 10 min).

After the first centrifugation, the push-pull rod 12 is mounted on the sealing head 11, the plug 32 is detached from the first luer 30, if for repairing bone and cartilage tissues, the push-pull rod 12 is pushed to discharge red blood cells from the first luer 30 to the corresponding scale (for example, 2mm), so that trace red blood cells can be accurately retained, then the plug 32 is used again to seal the first luer 30, the push-pull rod 12 is detached, and the syringe tube body is placed in a centrifuge for the second centrifugation (the centrifugation radius is 18cm, the rotation speed is 2300r/min, and the centrifugation time is 10 min); for beauty treatment and soft tissue repair, the red blood removing needle 34 is connected to the first luer 30, the push-pull rod 12 is pushed to discharge red blood cells, the liquid level of the red blood cells is located 1mm below the connection position of the red blood removing needle 34 and the first luer 30, so that the red blood cells are accurately removed, the red blood cells and part of other cells mixed in the red blood cells can be accurately removed by using the red blood removing needle 34 in cooperation with the beauty treatment and soft tissue repair, and the red blood cell-free PRP special for the beauty treatment and the soft tissue repair can be obtained.

Referring to fig. 6, the reddening needle 34 includes a connection end 341 and a tube body 342, the connection end 341 has a connector adapted to the first luer 30 and is detachably connected to the first luer 30, and the tube body 342 is connected to the connection end 341. The connection end 341 and the tube 342 are made of transparent material, so that the position of the red blood cells in the red blood removing needle 34 can be observed. In order to facilitate the detachment of the connection end 341, bar-shaped handles 343 may be respectively disposed at both sides of the connection end 341.

After the second centrifugation, the push-pull rod 12 is mounted on the sealing head 11, the plug 32 is removed from the first luer 30 and connected to the L-shaped transparent two-way connector 33, and the other end of the L-shaped transparent two-way connector 33 is connected to the container, so that a corresponding amount of PRP can be obtained.

According to the embodiment of the present disclosure, a first tube body 10 and a second tube body 20 are sequentially connected, and a sealing head 11 capable of moving up and down inside the first tube body 10 is disposed inside the first tube body 10, so that blood is sucked into the syringe through an opening structure at the bottom end of the second tube body 20 by the up-and-down movement of the sealing head 11, and is sealed through a first luer 30 for subsequent centrifugal treatment, and plasma is treated in a closed environment in the whole process, thereby preventing contamination caused by contamination such as external dust and bacteria mixed into the plasma. Meanwhile, the side wall of the second tube body section 20 is in arc adduction, the cross-sectional area of the second tube body section 20 is gradually reduced from being close to the first tube body section 10 to being far away from the first tube body section 10, the waist-contracting type structure can increase the height of red blood cells with the same volume compared with the cone-shaped structure, and meanwhile, the obvious light refraction can be generated under the irradiation of light such as sunlight or light, so that the position of the red blood cells can be observed more clearly, and the method is favorable for reminding a preparation worker to avoid excessive red removal (excessive red removal can cause excessive platelet removal, so that the prepared PRP concentration is reduced, the repairing effect of bones and cartilages is influenced), and then the red blood cells are removed more accurately.

In another aspect of the present disclosure, there is also provided a PRP preparation method, comprising the steps of:

step 1, draw the required blood using the above-described set-up single use PRP preparation syringe.

Specifically, the push-pull rod 12 is mounted on the sealing head 11, the blood collection needle 31 is connected to the first luer 30, and the push-pull rod 12 is pulled to collect blood to a desired amount of blood.

And 2, putting the assembled disposable PRP preparation syringe into a centrifuge for first centrifugation treatment, and discharging all red blood cells or most of red blood cells in plasma.

Specifically, before the first centrifugation, the push-pull rod 12 is removed from the sealing head 11, the blood collection needle 31 is removed from the first luer 30, and the plug 32 is used to seal the first luer 30, so that the syringe can be clamped in the centrifuge without shaking during the centrifugation.

The first centrifugation treatment can be carried out by centrifuging for 10min with a centrifuge radius of 18cm and a rotation speed of 2000 r/min.

After the first centrifugation, the push-pull rod 12 is attached to the sealing head 11, the plug 32 is removed from the first luer 30, and the push-pull rod 12 is pushed to expel the red blood cells from the first luer 30, so that most of the red blood cells in the plasma can be expelled.

When all red blood cells in plasma are discharged, the push-pull rod 12 is arranged at the sealing head 11, the red blood removing needle 34 is connected to the first luer 30, the push-pull rod 12 is pushed to discharge the red blood cells, and the liquid level of the red blood cells is positioned 1mm below the connection position of the red blood removing needle 34 and the first luer 30, so that all the red blood cells in the plasma can be discharged.

And 3, performing secondary centrifugation treatment on the residual blood after the primary centrifugation treatment to obtain the required PRP.

Before the second centrifugation, the push-pull rod 12 is removed again and the first luer fitting 30 is sealed with the stopper 32 and then placed in the centrifuge.

The second centrifugation treatment can be performed by centrifuging for 10min with a centrifuge radius of 18cm and a rotation speed of 2300 r/min.

After the second centrifugation, the push-pull rod 12 is attached to the sealing head 11, the plug 32 is removed from the first luer 30, an L-shaped transparent two-way connector 33 is connected to the first luer 30, a container is connected to the other end of the L-shaped transparent two-way connector 33, and the push-pull rod 12 is pushed to discharge PRP into the container.

According to the embodiment of the disclosure, in the whole process of preparing PRP, the plasma is treated in a closed environment, so that the pollution caused by the contamination of contaminants such as external dust and bacteria mixed in the plasma is avoided.

In the description of the present specification, the terms "connect", "mount", "fix", and the like are to be understood in a broad sense, for example, "connect" may be a fixed connection, a detachable connection, or an integral connection; may be directly connected or indirectly connected through an intermediate. The specific meaning of the above terms in the present application can be understood by those of ordinary skill in the art as appropriate.

In the description of the present application, the description of the terms "one embodiment," "some embodiments," etc. means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the application. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The above description is only a preferred embodiment of the present application and is not intended to limit the present application, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, improvement and the like made within the spirit and principle of the present application shall be included in the protection scope of the present application.

Claims

1. A modular single use PRP preparation syringe comprising:

the first pipe section is cylindrical, and the top end of the first pipe section is of an open structure;

the side wall of the second pipe body section is in arc-shaped adduction, the cross-sectional area of the second pipe body section is gradually reduced from the position close to the first pipe body section to the position far away from the first pipe body section, and the bottom end of the second pipe body section is of an open structure;

the sealing head is arranged in the first pipe body section, divides the first pipe body section into an upper chamber and a lower chamber, and can move up and down in the first pipe body section;

the first luer connector is arranged at the bottom end opening of the second pipe body section.

2. The modular single use PRP preparation syringe according to claim 1,

the sealing head is detachably connected with a push-pull rod, and the push-pull rod can push and pull the sealing head to enable the sealing head to move up and down in the first pipe body section.

3. The modular single use PRP preparation syringe according to claim 2,

the push-pull rod comprises a rod part and a connecting part, a second Ruhr joint is arranged on the upper surface of the sealing head, and the connecting part is connected with the second Ruhr joint.

4. The modular single use PRP preparation syringe according to claim 1,

the distance of the arc-shaped vertex of the arc-shaped side wall of the second pipe body section is 4mm, and the scales are marked on the second pipe body section at the positions of 2mm, 4mm and/or 6mm away from the bottom end of the second pipe body section in the axial direction.

5. The modular single use PRP preparation syringe according to claim 4,

the height of the second tube section is 8 mm.

6. The modular single use PRP preparation syringe according to claim 1,

the periphery of the upper end of the first pipe body section is provided with a flange, and a circular ring-shaped handle is sleeved on the flange.

7. The modular single use PRP preparation syringe according to claim 1,

and the inner wall of the first pipe section and/or the inner wall of the second pipe section are subjected to mirror grinding process treatment, and the surface roughness of the inner wall is less than or equal to 0.01 micrometer.

8. The modular single use PRP preparation syringe according to claim 1,

the first tube section has a height of 118mm and an inner diameter of 28 mm; the first pipe body section is marked with scales and a volume.

9. The modular single use PRP preparation syringe of claim 1, further comprising:

one or more of a blood taking needle, a plug, an L-shaped transparent double-way connector and a red removing needle are used for being connected to the luer connector.

10. A method of preparing PRP, comprising:

drawing a desired blood using the set-up single use PRP preparation syringe of any one of claims 1 to 9;

placing the assembled disposable PRP preparation syringe into a centrifuge for first centrifugation treatment, and discharging red blood cells or most of red blood cells in blood;

and (4) carrying out second centrifugation treatment on the residual blood after the first centrifugation treatment to obtain the required PRP.