CN114097595A - Culture method for cabbage seedling under weak light condition - Google Patents

Culture method for cabbage seedling under weak light condition Download PDF

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Publication number
CN114097595A
CN114097595A CN202111414993.3A CN202111414993A CN114097595A CN 114097595 A CN114097595 A CN 114097595A CN 202111414993 A CN202111414993 A CN 202111414993A CN 114097595 A CN114097595 A CN 114097595A
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China
Prior art keywords
incubator
seedlings
hole
placing plate
low light
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CN202111414993.3A
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Chinese (zh)
Inventor
胡琳莉
郁继华
廖伟彪
张国斌
吕剑
肖雪梅
李雨桐
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Gansu Agricultural University
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Gansu Agricultural University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C1/00Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/15Leaf crops, e.g. lettuce or spinach 
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • A01G31/02Special apparatus therefor
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G31/00Soilless cultivation, e.g. hydroponics
    • A01G31/02Special apparatus therefor
    • A01G31/06Hydroponic culture on racks or in stacked containers
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/20Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
    • Y02P60/21Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Soil Sciences (AREA)
  • Botany (AREA)
  • Hydroponics (AREA)

Abstract

The invention belongs to the field of seedling culture, and particularly discloses a culture method for seedling culture of Chinese cabbages under a low-light condition, wherein when seedlings with two leaves and one core are formed after seeds germinate, the seedlings are transplanted into an incubator, the ratio of ammonium ions to nitrate ions in a culture solution in the incubator is 10:90, and nitric oxide is introduced into the culture solution. The scheme of the invention can be used for cultivating the seedling of the Chinese cabbage, and the seedling of the Chinese cabbage under the low-light condition is solved.

Description

Culture method for cabbage seedling under weak light condition
Technical Field
The invention belongs to the field of seedling culture, and particularly relates to a culture method for seedling culture of Chinese cabbages under a low-light condition.
Background
The weak light influences the growth and development process of plants, and is one of the main environmental factors causing yield reduction of horticultural crops and product quality reduction, but the sunlight intensity and the irradiation intensity are uncontrollable, so that the research on how to cultivate horticultural crops under the condition of the weak light (namely how to reduce the influence of the weak light on the horticultural crops) becomes a difficult problem.
Disclosure of Invention
The invention aims to provide a method for cultivating seedlings of Chinese cabbages under the condition of weak light, which aims to solve the problem of cultivating horticultural crops under the condition of weak light.
In order to achieve the purpose, the basic scheme of the invention is as follows: a method for culturing the seedlings of Chinese cabbage under weak light condition includes such steps as germinating the seeds to form two-leaf and one-core seedlings, transplanting the seedlings to an incubator with the ammonium ions and nitrate ions in the culture liquid in the incubator in the ratio of 10:90, and introducing nitric oxide to the culture liquid.
The theory of operation and the beneficial effect of this basic scheme lie in:
experiments prove that the proper ammonium nitrate proportion can improve the activity of antioxidase of small Chinese cabbage seedlings, improve the capacity of removing lipid peroxidation substances of plants, and reduce the damage to the plants caused by adverse circumstances, and meanwhile, the damage of weak light to the small Chinese cabbage seedlings is further relieved by introducing nitric oxide.
Further, the seeds are pregerminated in an artificial climate box and then sown so that the seeds germinate.
Has the advantages that: the germination is accelerated through the artificial climate box, and the seed cultivation efficiency is improved.
Further, the seeds were sown in a plug tray filled with quartz sand.
Has the advantages that: the seeds are limited to a certain extent by the quartz sand, and after the seeds germinate, the quartz sand also limits the roots of the seedlings to a certain extent.
Furthermore, the seedlings need to be revived in distilled water for 1-2 days before being transplanted to an incubator.
Has the advantages that: the seedlings are adapted to the water culture environment and then transplanted into the culture solution.
Further, the incubator comprises a transparent box body, a placing plate is vertically and slidably connected in the box body, connecting ropes are fixed on two sides of the placing plate, a rotary drum used for winding the connecting ropes is rotatably connected to the upper end of the box body, a handle is fixed on the end face of the rotary drum, and one end, far away from the placing plate, of each connecting rope is fixed on the rotary drum; the placing plate is provided with a plurality of mounting holes, the mounting holes are internally inserted with the hole discs, and the placing plate is provided with a fixing mechanism for fixing the hole discs; the side wall of the plug is provided with a plurality of through holes, the two sides of the box body are respectively provided with a liquid inlet hole and a liquid outlet hole, and a circulating pipe is communicated between the liquid inlet hole and the liquid outlet hole.
Has the advantages that: 1. the Chinese cabbage seedlings germinate in the hole trays, the hole trays are placed on the placing plate, and the hole trays are fixed on the placing plate through the fixing mechanism. And introducing the culture solution into the box body, wherein the culture solution is contacted with the roots of the Chinese cabbage seedlings through the through holes on the plug tray. Operating personnel grips the handle and rotates the rotary drum, and the rotary drum winding or loosen and connect the rope, and then makes to connect the rope and drive and place the board and slide in the box, adjusts the contact range of cave dish lower extreme and culture solution, and the box sets up to the transparence, and the operating personnel of being convenient for observes.
2. Through the slip of placing the board, can slide to box upper portion placing the board when transplanting, then insert the mounting hole with the cave dish again, be convenient for the transplantation of cave dish.
3. The circulating pipe is communicated between the liquid inlet hole and the liquid outlet hole, so that the culture solution circulates back and forth in the culture process, the culture solution is in a flowing state, and the roots of the Chinese cabbage seedlings in the plug are better contacted with the culture solution.
Further, the cave dish is including being the cup and fixing the ring form spacing ring that is in cup upper end, and the external diameter of spacing ring is greater than the internal diameter of mounting hole, cup and mounting hole clearance fit, and fixed establishment includes sliding connection and places the inserted block on the board, is equipped with on the spacing ring to supply inserted block male jack.
Has the advantages that: the plug tray is clamped in the mounting hole through the limiting ring, so that the structure is simple and the processing is convenient; the insertion block is inserted into the insertion hole, and the circumferential rotation of the plug tray can be limited.
Furthermore, the insert block is higher than the limiting ring, pressing blocks are fixed on two sides of the upper end of the insert block, and after the insert block is inserted into the jack, the pressing blocks are located above the limiting ring and press the limiting ring.
Has the advantages that: the pressing block presses the limiting ring to limit the vertical movement of the plug tray.
Drawings
FIG. 1 is a front sectional view of an incubator used in an example of the present invention.
Fig. 2 is a side view of the drum of fig. 1.
Fig. 3 is a top view of the placement plate of fig. 1.
Detailed Description
The following is further detailed by way of specific embodiments:
reference numerals in the drawings of the specification include: the cup comprises a box body 1, a placing plate 2, a cup body 3, a limiting ring 4, a plug disk 5, a handheld ring 6, a connecting rope 7, a rotary drum 8, a liquid inlet hole 9, a liquid outlet hole 10, a sliding groove 11, a sliding block 12, a supporting rod 13, a limiting block 14, an inserting block 15 and a pressing block 16.
Example (b):
the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The cultivation method for Chinese cabbage seedling under the condition of weak light is characterized by that the seeds are placed in an artificial climate box to accelerate germination, then the seeds are sowed in a hole tray filled with quartz sand to make germination. When the seeds germinate to form seedlings with two leaves and one core, transplanting the seedlings into distilled water for 1-2 days. Then transplanting the seedlings into an incubator, wherein the ratio of ammonium ions to nitrate ions in a culture solution in the incubator is 10:90, and introducing nitric oxide into the culture solution (the culture solution in the invention comprises other element ions required by seedling growth, and the existence form of nitrogen in the culture solution is only limited in the application).
As shown in FIG. 1, the incubator used in the above step includes a transparent box body 1, a placing plate 2 is vertically and slidably connected in the box body 1, specifically, a vertical sliding groove 11 is formed in the inner wall of the box body 1, and a sliding block 12 slidably connected in the sliding groove 11 is fixed on the placing plate 2.
The both sides of placing board 2 all are fixed with and connect rope 7, and 1 upper end fixedly connected with branch 13 of box rotates on branch 13 and is connected with the rotary drum 8 that is used for twining to connect rope 7, and 8 terminal surfaces of rotary drum are fixed with the handle, connect rope 7 to keep away from the one end of placing board 2 and fix on rotary drum 8, combine shown in fig. 2, are equipped with a plurality of spacing holes along circumference on the rotary drum 8, have along vertical sliding connection on the box 1 and are used for inserting spacing downthehole stopper 14.
Place and be equipped with a plurality of mounting holes on the board 2, it has cave dish 5 to peg graft in the mounting hole, and cave dish 5 is the annular spacing ring 4 of circle including being cup 3 and fixing in cup 3 upper end, and the external diameter of spacing ring 4 is greater than the internal diameter of mounting hole, and cup 3 and mounting hole clearance fit, the both sides of spacing ring 4 all are fixed with handheld ring 6. Place and be equipped with the fixed establishment who is used for fixed cave dish 5 on the board 2, combine as shown in fig. 3, fixed establishment includes sliding connection at the inserted block 15 of placing on the board 2, is equipped with on the spacing ring 4 and supplies inserted block 15 male jack, and the height of inserted block 15 is higher than spacing ring 4, and 15 upper end both sides of inserted block all are fixed with briquetting 16, and after inserted block 15 inserted the jack, briquetting 16 was located the spacing ring 4 top and pushed down spacing ring 4.
The side wall of the cave disk 5 is provided with a plurality of through holes, the two sides of the box body 1 are respectively provided with a liquid inlet hole 9 and a liquid outlet hole 10, a circulating pipe (not shown in the figure) is communicated between the liquid inlet hole 9 and the liquid outlet hole 10, and the middle part of the circulating pipe is communicated with a circulating pump.
During specific use, the cabbage seedling sprouts the back in cave dish 5, places cave dish 5 on placing board 2, can will place board 2 and slide to 1 upper portion of box when transplanting, and is concrete, and operating personnel grips the handle and rotates rotary drum 8, and rotary drum 8 twines or loosens and connects rope 7, and then makes to connect rope 7 to drive and places board 2 and slide in box 1, then inserts the cave dish 5 in the mounting hole again, the transplantation of the cave dish 5 of being convenient for.
The plug 5 is fixed on the placing plate 2 through the fixing mechanism, specifically, the insert block 15 is inserted into the insertion hole, so that the circumferential rotation of the plug 5 can be limited, the limit ring 4 is pressed through the press block 16, and the vertical movement of the plug 5 is limited. And (3) introducing the culture solution into the box body 1, wherein the culture solution is contacted with the roots of the Chinese cabbage seedlings through the through holes on the plug 5. Operating personnel grips the handle and rotates rotary drum 8, and rotary drum 8 winding or loosen and connect rope 7, and then makes to connect rope 7 to drive and places board 2 and slide in box 1, adjusts the contact range of 5 lower extremes of cave dish and culture solution, then slides stopper 14 for stopper 14 inserts apart from its nearest spacing downthehole, carries on spacingly to rotary drum 8. The box body 1 is transparent, so that an operator can observe the box body conveniently. In the culture process, the culture solution circulates back and forth through the arrangement of the circulating pump and the circulating pipe, and the culture solution is in a flowing state, so that the roots of the cabbage seedlings in the plug tray 5 are better contacted with the culture solution.
The set-up experiment was as follows:
comparative example 1: the nitrogen element in the culture solution exists in a form of full nitrate nitrogen.
Comparative example 2: the nitrogen element in the culture solution exists in the form of nitric oxide.
Comparative example 3: the existence form of nitrogen element in the culture solution is as follows: the ratio of ammonium ions to nitrate ions is 10:90, and a nitric oxide scavenger is added.
Comparative example 4: the existence form of nitrogen element in the culture solution is as follows: the ratio of ammonium ions to nitrate ions is 10:90, and a nitric oxide inhibitor is added.
Comparative example 5: the only differences from the examples are: no nitric oxide was introduced.
Comparative example 6: the only differences from the examples are: the ratio of ammonium ions to nitrate ions was 20: 80.
Comparative example 7: the only differences from the examples are: the ratio of ammonium ions to nitrate ions was 30: 70.
In the comparative example, the setup was identical except that the form of nitrogen element in the culture solution was different. After the seedlings were cultured in the incubator for 8 days, the seedlings were observed and dissected, and the test results are shown in the following table:
Figure BDA0003374930080000041
Figure BDA0003374930080000051
and (4) test conclusion:
1. suitable ammonium nitrate ratio (NH)4 +:NO3 -10: 90) can improve the growth and development of the small Chinese cabbage seedlings under weak light. Under weak light, compared with full nitrate nitrogen treatment, the addition of ammonium nitrogen with proper concentration in the nutrient solution can promote the development of lateral roots, improve the configuration of the root system, increase the absorption area of the root system and facilitate the plants to absorb water and nutrient substances; on the other hand, the stacking degree of chloroplast basal granule sheet layers can be increased, the integrity of chloroplast structures is protected, the photosynthesis of plants is improved, and the growth and development of the plants are further improved.
2. The anti-oxidation mechanism suitable for relieving the weak light stress of the small Chinese cabbage seedlings by the ammonium nitrate ratio is as follows: under weak light, the proper ammonium nitrate proportion can improve the activity of antioxidase of small Chinese cabbage seedlings, improve the capability of removing lipid peroxidation substances of plants and reduce the damage to the plants caused by adverse circumstances.
4. Meanwhile, the damage of weak light to the small Chinese cabbage seedlings can be relieved by introducing nitric oxide into the culture solution, however, the effect of the ammonium nitrate nutrition on the reduction of the small Chinese cabbage seedling growth under the weak light is obviously reduced by adding a nitric oxide inhibitor (L-NAME and NaN 3) and a scavenger (Hb) into the proper ammonium nitrate nutrition, and therefore the regulation effect of NO participating in the ammonium nitrate nutrition on the small Chinese cabbage seedling growth under the weak light is proved.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
The foregoing is merely an example of the present invention, and common general knowledge in the field of known specific structures and characteristics is not described herein in any greater extent than that known in the art at the filing date or prior to the priority date of the application, so that those skilled in the art can now appreciate that all of the above-described techniques in this field and have the ability to apply routine experimentation before this date can be combined with one or more of the present teachings to complete and implement the present invention, and that certain typical known structures or known methods do not pose any impediments to the implementation of the present invention by those skilled in the art. It should be noted that, for those skilled in the art, without departing from the structure of the present invention, several changes and modifications can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.

Claims (7)

1. The culture method for cabbage seedling under the condition of weak light is characterized by comprising the following steps of: when the seeds germinate to form seedlings with two leaves and one heart, the seedlings are transplanted into an incubator, the ratio of ammonium ions to nitrate ions in a culture solution in the incubator is 10:90, and nitric oxide is introduced into the culture solution.
2. A method for cultivating cabbage seedlings under low light conditions as claimed in claim 1, characterized in that: the seeds are pregerminated in an artificial climate box and then sown so that the seeds germinate.
3. The culture method for cabbage seedling under the low light condition as claimed in claim 2, characterized in that: the seeds are sown in a plug tray filled with quartz sand.
4. A method as claimed in claim 3, for cultivating cabbage seedlings under low light conditions, characterized in that: the seedlings need to be revived in distilled water for 1-2 days before being transplanted to an incubator.
5. A method for cultivating cabbage seedlings under low light conditions as claimed in claim 4, characterized in that: the incubator comprises a transparent incubator body, a placing plate is vertically and slidably connected in the incubator body, connecting ropes are fixed on two sides of the placing plate, a rotary drum used for winding the connecting ropes is rotatably connected to the upper end of the incubator body, a handle is fixed on the end face of the rotary drum, and one end, far away from the placing plate, of each connecting rope is fixed on the rotary drum; the placing plate is provided with a plurality of mounting holes, the mounting holes are internally inserted with the hole discs, and the placing plate is provided with a fixing mechanism for fixing the hole discs; the side wall of the plug is provided with a plurality of through holes, the two sides of the box body are respectively provided with a liquid inlet hole and a liquid outlet hole, and a circulating pipe is communicated between the liquid inlet hole and the liquid outlet hole.
6. A method for cultivating cabbage seedlings under low light conditions as claimed in claim 5, characterized in that: the cave dish is including being the cup and fixing the ring form spacing ring that is in cup upper end, and the external diameter of spacing ring is greater than the internal diameter of mounting hole, cup and mounting hole clearance fit, and fixed establishment includes sliding connection and places the inserted block on the board, is equipped with on the spacing ring and supplies inserted block male jack.
7. A method for cultivating cabbage seedlings under low light conditions as claimed in claim 6, characterized in that: the height of the insert block is higher than that of the limiting ring, pressing blocks are fixed on two sides of the upper end of the insert block, and after the insert block is inserted into the insertion hole, the pressing blocks are located above the limiting ring and press the limiting ring.
CN202111414993.3A 2021-11-25 2021-11-25 Culture method for cabbage seedling under weak light condition Withdrawn CN114097595A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115517135A (en) * 2022-10-10 2022-12-27 山东友和生物科技股份有限公司 A microcirculation analogue means for fungus mushroom

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115517135A (en) * 2022-10-10 2022-12-27 山东友和生物科技股份有限公司 A microcirculation analogue means for fungus mushroom
CN115517135B (en) * 2022-10-10 2023-10-17 山东友和生物科技股份有限公司 Microcirculation simulation device for mushrooms

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