CN114053164A - Essence with anti-inflammatory, acne-removing and skin regeneration and repair promoting functions and preparation method thereof - Google Patents

Abstract

The invention discloses essence with anti-inflammatory, repairing and cell regeneration promoting functions and a preparation method thereof. The essence liquid takes PDRN as a main active component, and also comprises a plurality of active components such as a centella extract, an aloe extract, a mulberry leaf extract, a chamomile extract, a poria cocos sclerotium extract and the like. The PDRN is extracted from the spermary of the salmon, has the size of 50-500 bp, has the effects of resisting inflammation, promoting the generation of collagen and promoting the regeneration of cells, can be transferred to the dermis layer of the skin, regulates the internal environment of the skin and fundamentally improves sensitive skin. The effective components in the essence act together, and the obvious functions of removing acne, diminishing inflammation and promoting cell regeneration are shown.

Description

Essence with anti-inflammatory, acne-removing and skin regeneration and repair promoting functions and preparation method thereof

Technical Field

The invention relates to an essence with anti-inflammatory, acne-removing and skin regeneration and repair promoting functions and a preparation method thereof, and belongs to the technical field of biomedicine and skin care products.

Background

Acne, also known as pox or whelk, affects 85% of adolescents, usually beginning before puberty and continuing until adulthood. Acne and the resulting post-inflammatory pigmentation have profound negative effects on adolescent self-cognition, social interaction, and quality of life scores. In addition, pox still appears in many adults now, and the duration is long and difficult to recover, influences people's outward appearance and mental health to further produce negative effects to the life of working. The factors causing the growth of pox in life include ultraviolet rays, long-term consumption of irritant food, overeating, staying up in the night and the like. With the increasing pressure of people in life and work, people have stronger requirements on cosmetics with the functions of effectively removing acnes, diminishing inflammation and promoting cell regeneration.

Polydeoxyribonucleotides (PDRNs) are natural low-molecular-weight DNA molecules with the length of 50-2000 bp, and the natural sources of the PDRNs are mainly human placenta or trout sperms. PDRNs bind to water molecules to form a 3D gel and are cleaved into nucleotides, which naturally occur in the ECM and are used by cells for metabolism. In addition to being involved in cell growth, PDRNs promote the production of Vascular Endothelial Growth Factor (VEGF) and collagen, the migration of various cells, and reduce inflammatory responses. Both increased VEGF production and decreased inflammation are associated with promoting tissue healing. The major mechanism by which PDRNs are known to function is their interaction with adenosine receptors, particularly the A2A receptor. Based on its multiple effects, it has been used to improve the skin repair process, enhance the wound healing strength, tissue repair, wound regeneration and skin beauty of diabetic people. However, much of the research on PDRN and the products currently on the market has been focused abroad. Reports and related products in this respect are rare in China. The invention provides a formula and a preparation method of an essence containing PDRN active ingredients and having acne removal and skin repair functions based on a traditional Chinese medicine beautifying formula, and Chinese elements are endowed in the application of PDRN, so that double experiences of beautiful face and Chinese culture are provided for users.

Disclosure of Invention

Technical problem to be solved

The invention provides essence with anti-inflammatory, acne-removing and skin regeneration and repair promoting functions and a preparation method thereof. The essence can remove acne and diminish inflammation, repair skin injury, promote skin metabolism and enable skin to be healthier and firmer. The essence disclosed by the invention contains PDRN active ingredients from the salmon testis and a plurality of Chinese herbal medicine active ingredients, has an obvious effect and high safety, and is suitable for people with sensitive skin.

The technical scheme adopted by the invention is as follows:

an essence with anti-inflammatory, repairing and cell regeneration promoting effects contains active ingredients of DAMA PDRN and multiple Chinese herbal medicine ingredients;

the essence is prepared from the following raw materials in parts by weight: 1-3 parts of PDRN, 3-5 parts of herba centellae extract, 6-10 parts of aloe extract, 4-8 parts of mulberry leaf extract, 1-6 parts of chamomile extract, 1-5 parts of poria cocos sclerotium extract, 2-8 parts of hyaluronic acid and 44-75 parts of cosmetic matrix.

Further, the essence mainly comprises the following components in parts by weight: 2 parts of PDRN, 4 parts of centella extract, 8 parts of aloe extract, 4 parts of mulberry leaf extract, 3 parts of chamomile extract, 2 parts of poria cocos sclerotium extract, 3 parts of hyaluronic acid and 74 parts of cosmetic matrix.

Further, the preparation method of PDRN comprises the following steps:

step 1: taking out the salmon testis, and cleaning the testis with pre-cooled normal saline;

step 2: adding extraction buffer solution into the spermary tissue, and crushing homogenate by using a crusher;

and step 3: adding protease K for enzymolysis of protein;

and 4, step 4: centrifuging to remove the precipitate, and collecting the supernatant;

and 5: adding saturated sodium chloride solution, stirring uniformly, centrifuging and collecting supernatant;

step 6: adding absolute ethyl alcohol, and precipitating the fish spermary DNA at low temperature;

and 7: collecting the precipitate and washing twice with 75% ethanol;

and 8: the precipitate (DNA) was dissolved in deionized water and then disrupted by ultrasonication.

And step 9: freeze drying to obtain small molecule Polydeoxyribonucleotide (PDRN).

Further, the preparation method of the extract in the essence comprises the following steps: respectively drying herba Centellae, Aloe, folium Mori, flos Matricariae Chamomillae, and Poria sclerotium, and pulverizing; then adding deionized water and ethanol, and uniformly stirring; centrifuging, collecting supernatant, filtering, decolorizing and flocculating to obtain clear solution, aging, and filtering to obtain essence extract.

Further, the cosmetic matrix mainly comprises the following components: one or more of emulsifier, humectant, thickener, antiseptic, emollient, and essence.

Further, the blending step of the essence comprises the following steps:

A. adding extracts of centella, aloe, mulberry leaf, chamomile and poria cocos sclerotium into a cosmetic matrix according to parts by weight, and uniformly stirring.

B. And (3) adding the extracted PDRN into the mixed extract A according to the parts by weight, and fully and uniformly mixing to obtain the essence.

The beneficial effects are that:

the essence mainly adopts PDRN from the spermary of fresh salmon and a natural pure plant Chinese herbal medicine formula, and can effectively relieve acnes, eliminate skin inflammation, repair damaged skin and enable the skin to be smooth and elastic.

Detailed Description

An essence with anti-inflammatory, repairing and cell regeneration promoting effects contains active ingredients of DAMA PDRN and multiple Chinese herbal medicine ingredients;

the essence is prepared from the following raw materials in parts by weight: 1-3 parts of PDRN, 3-5 parts of herba centellae extract, 6-10 parts of aloe extract, 4-8 parts of mulberry leaf extract, 1-6 parts of chamomile extract, 1-5 parts of poria cocos sclerotium extract, 2-8 parts of hyaluronic acid and 44-75 parts of cosmetic matrix.

Among them, PDRN was extracted from the testis of salmon, and since it was found in italy, a number of experimental and clinical studies have been carried out, and its remarkable features are excellent skin repair, wound healing enhancement, tissue repair, and wound regeneration. It has a high similarity to human DNA sequence of 98%. The extraction steps of the PDRN are as follows:

step 1: taking out the salmon testis, cleaning the testis with precooled normal saline, and washing off blood;

step 2: adding testis tissue into 4-5 times volume of extraction buffer solution (containing Tris-HCl, NaCl, Na2EDTA), and crushing homogenate with a crusher;

and step 3: adding appropriate amount of protease K, performing enzymolysis for protein at 30-40 deg.C for 12-20h, centrifuging (10000rpm,30min) to remove precipitate, and collecting supernatant;

and 4, step 4: adding saturated sodium chloride solution, stirring, centrifuging (10000rpm,30min), and collecting supernatant;

and 5: adding equal volume of absolute ethyl alcohol, and precipitating the fish spermary DNA at low temperature;

step 6: centrifuging at low temperature to collect precipitate (10000rpm,10min), washing with 75% ethanol twice, centrifuging to collect precipitate;

and 7: the precipitate (DNA) is dissolved in deionized water and then the DNA is disrupted by ultrasonic disruption.

And 8: freeze drying to obtain small molecule Polydeoxyribonucleotide (PDRN).

The extract used by the essence comprises a centella extract, an aloe extract, a mulberry leaf extract, a chamomile extract and a poria cocos sclerotium extract. The functions of the raw materials are introduced as follows:

centella asiatica: has effects in promoting diuresis, relieving swelling, clearing away heat and toxic materials, relieving inflammation, and removing abscess. Can stimulate cell replacement, promote collagen formation, and make skin compact and smooth. It also has effects in inhibiting increase of fat cells and preventing edema. In addition, the extract has antibacterial and anti-inflammatory effects, and can relieve swelling, remove toxic substance and remove abscess.

Aloe vera: sterilizing, antiinflammatory, antiaging, moisturizing, and sunscreen effects.

Mulberry leaf: has effects of dispelling pathogenic wind and heat, clearing lung-heat, moistening dryness, removing liver fire, and improving eyesight. In addition, the mulberry leaves also have good efficacies of improving color spots and acne.

Chamomile: is a good skin care product, and has effects of promoting wound healing of blister and ulcer. Can improve sensitive skin such as eczema and herpes, and repair ruptured capillary.

Tuckahoe, poria cocos: removing speckle, whitening skin, enhancing immunity, dilating blood vessel, and treating pyocutaneous disease and erysipelas.

The preparation method of the extracts of the raw materials comprises the following steps: drying and pulverizing the raw materials respectively. Then adding a mixture of deionized water and ethanol with the mass 6 times of that of the raw materials, and uniformly stirring. Centrifuging, collecting supernatant, filtering, decolorizing and flocculating to obtain clear solution, aging, and filtering to obtain extract of each raw material.

The extracted PDRN, the Chinese herbal medicine extract and the purchased cosmetic matrix are weighed according to the following parts by weight to prepare the essence with the functions of resisting inflammation, repairing and promoting cell regeneration: adding 3-5 parts of asiatic pennywort herb extracting solution, 6-10 parts of aloe extracting solution, 4-8 parts of mulberry leaf extracting solution, 1-6 parts of chamomile extracting solution, 1-5 parts of poria cocos sclerotium extracting solution and 2-8 parts of hyaluronic acid into a cosmetic matrix, uniformly mixing, stabilizing, adding 1-3 parts of PDRN, and obtaining the uniformly mixed solution, namely the essence disclosed by the invention.

The essence mainly adopts PDRN from the spermary of fresh salmon and a natural pure plant Chinese herbal medicine formula, and can effectively relieve acnes, eliminate skin inflammation, repair damaged skin and enable the skin to be smooth and elastic.

The invention is illustrated in detail below with reference to examples:

the essence with the functions of resisting inflammation, repairing and promoting cell regeneration comprises the following components in parts by weight: 2 parts of PDRN, 4 parts of centella extract, 8 parts of aloe extract, 4 parts of mulberry leaf extract, 3 parts of chamomile extract, 2 parts of poria cocos sclerotium extract, 3 parts of hyaluronic acid and 74 parts of cosmetic matrix. The components are fully and uniformly mixed to obtain the essence with the functions of resisting inflammation, repairing and promoting cell regeneration.

The cosmetic base used in the present embodiment includes the following main components: 5% of glycerin, 5% of sorbitol, 0.2% of carbomer (0.2%), 0.2% of polyethylene glycol, 0.2% of allantoin, 3% of hydroxyethyl urea, 0.2% of triethanolamine, 0.15% of phenoxyethanol, 0.05% of essence and the balance of deionized water.

The preparation method of the essence in the embodiment is as follows:

the method comprises the following steps: PDRN is extracted from the salmon testis. Taking out the salmon testis, cleaning the testis with precooled normal saline, and washing off blood; weighing 100g of spermary tissue, adding 5 times of extraction buffer solution (Tris-HCl, NaCl, Na2EDTA), and crushing homogenate by using a crusher; adding 4mg proteinase K, performing enzymolysis at 37 deg.C for 18h, centrifuging at 10000rpm for 30min to remove precipitate, and collecting supernatant; adding an equal volume of saturated sodium chloride solution, stirring uniformly, centrifuging at 10000rpm for 30min, and collecting supernatant; adding equal volume of absolute ethyl alcohol into the supernatant, and precipitating the fish spermary DNA at low temperature; washing the DNA obtained by precipitation twice by using 75% ethanol, and centrifugally collecting precipitate (DNA); dissolving the collected DNA with deionized water, and breaking the DNA by an ultrasonic crushing method; freeze drying to obtain small molecule Polydeoxyribonucleotide (PDRN).

The obtained precipitate is micromolecular polydeoxyribonucleotide.

Step two: and (3) preparing a Chinese herbal medicine extracting solution. Drying herba Centellae, Aloe, folium Mori, flos Matricariae Chamomillae, and Poria sclerotium respectively, and pulverizing. Then deionized water and ethanol with the mass 6 times of that of the raw materials are added and stirred uniformly. Centrifuging, collecting supernatant, filtering, decolorizing and flocculating to obtain clear liquid, aging, and filtering to obtain extractive solutions of the raw materials.

Step three: and (5) blending the essence. Sequentially adding 4 parts of herba centellae extract, 8 parts of aloe extract, 4 parts of mulberry leaf extract, 3 parts of chamomile extract, 2 parts of poria cocos sclerotium extract and 3 parts of hyaluronic acid into 74 parts of cosmetic matrix, uniformly and stably mixing, adding 2 parts of PDRN, and obtaining the uniformly mixed solution, namely the essence disclosed by the invention.

Test (1): according to the cosmetic hygiene Specification 2007, an in-vitro test of a skin irritation experiment is carried out, and the inhibition effect of the essence on propionibacterium acnes is detected. The experiment is divided into an essence group, an essence group without PDRN components and a cosmetic group with the function of acne removal repair sold in the market. The result shows that the propionibacterium acnes is very sensitive to the essence disclosed by the invention, namely the essence has an obvious inhibition effect on the propionibacterium acnes.

Test (2): lipopolysaccharide LPS-induced RAW264.7 mouse macrophage model anti-inflammatory test: the experiment is divided into an essence group, an essence group without PDRN components and a cosmetic group with the function of acne removal and repair sold in the market. The RAW264.7 mouse macrophages were inoculated into 24-well plates and cultured routinely for 24h, then the old medium was discarded and washed 2 times with PBS. The corresponding test substances are added into each group respectively for post-treatment for 1 h. After the treatment, the culture medium containing LPS (5mg/L) was added for further incubation for 16 h. The supernatant was collected by centrifugation and the TNF-. alpha.content was measured using an ELISA kit. The result shows that the essence disclosed by the invention can more effectively inhibit the secretion of inflammatory factors compared with the essence group without PDRN components and cosmetics with the acne-removing and repairing functions sold in the market.

Testing (3) evaluation of the skin repair effect of the human body for removing acnes:

the use method comprises the following steps: after the face is cleaned every day, a proper amount of essence is applied to the face and the pox respectively in the morning and at night. The observation was performed once a day.

The experimental subjects were 30 men 25-30 years old, often left overnight, with irregular diet and pox marks on the face.

Grouping experiments: the cosmetic composition comprises an essence group, an essence group without PDRN components and a cosmetic group with the function of acne removal and repair in the market. Each group was randomly assigned 10 people.

And (4) trial results: after 3 days of use, 7 pockmarks in the essence group are obviously relieved, and the red swelling is relieved; 5-person pox is relieved and red swelling is relieved in the essence group without PDRN components; the commercially available cosmetic group with the acne removing and repairing functions has 5-person acne relieving effects. After one week, the essence group 7 acnes basically disappear, the acne marks are reduced, and the skin is obviously smoother and more elastic than before; the acne of 3 people is obviously relieved, and the red swelling of the skin disappears. The essence group without PDRN component had 3 persons with pox basically disappeared, and 7 persons had different degrees of remission. The commercially available cosmetic group with the acne removing and repairing functions basically eliminates 4 acnes, obviously relieves 6 acnes and smoothens the skin.

The foregoing has described the general principles, major features and advantages of the invention. It should be understood by those skilled in the art that the present invention is not limited to the above-described embodiments, and various modifications and improvements can be made without departing from the principle and scope of the present invention, and such changes are to be included in the scope of the present invention.

Claims (6)

1. The essence with the functions of resisting inflammation, removing acnes and promoting skin regeneration and repair is characterized by comprising the following raw materials in parts by weight: 1-3 parts of PDRN, 3-5 parts of centella asiatica extract, 6-10 parts of aloe extract, 4-8 parts of mulberry leaf extract, 1-6 parts of chamomile extract, 1-5 parts of poria cocos sclerotium extract, 2-8 parts of hyaluronic acid and 44-75 parts of cosmetic matrix.

2. The essence with the functions of resisting inflammation, removing acnes and promoting skin regeneration and repair according to claim 1, which is characterized by mainly comprising the following components in parts by weight: the cosmetic comprises, by weight, PDRN2 parts, centella asiatica extract 4 parts, aloe extract 8 parts, mulberry leaf extract 4 parts, chamomile extract 3 parts, poria cocos sclerotium extract 2 parts, hyaluronic acid 3 parts and a cosmetic matrix 74 parts.

3. The preparation method of the essence with the functions of resisting inflammation, removing acnes and promoting skin regeneration and repair according to claim 1, wherein the preparation method of PDRN is as follows:

step 1: taking out the salmon testis, and cleaning the testis with pre-cooled normal saline;

step 2: adding extraction buffer solution into the spermary tissue, and crushing homogenate by using a crusher;

and step 3: adding protease K for enzymolysis of protein;

and 4, step 4: centrifuging to remove the precipitate, and collecting the supernatant;

and 5: adding saturated sodium chloride solution, stirring uniformly, centrifuging and collecting supernatant;

step 6: adding absolute ethyl alcohol, and precipitating the fish spermary DNA at low temperature;

and 7: collecting the precipitate and washing twice with 75% ethanol;

and 8: the precipitate (DNA) was dissolved in deionized water and then disrupted by ultrasonication.

And step 9: freeze drying to obtain small molecule Polydeoxyribonucleotide (PDRN).

4. The essence for resisting inflammation, removing acnes and promoting skin regeneration and repair functions and the preparation method thereof according to claim 1, wherein the preparation method of the extract in the essence comprises the following steps: respectively drying herba Centellae, Aloe, folium Mori, flos Matricariae Chamomillae, and Poria sclerotium, and pulverizing; then adding deionized water and ethanol, and uniformly stirring; centrifuging, collecting supernatant, filtering, decolorizing and flocculating to obtain clear solution, aging, and filtering to obtain essence extract.

5. The essence with the functions of resisting inflammation, removing acnes and promoting skin regeneration and repair as well as the preparation method of the essence are characterized in that the cosmetic matrix mainly comprises the following components: one or more of emulsifier, humectant, thickener, antiseptic, emollient, and essence.

6. The essence with the functions of resisting inflammation, repairing and promoting cell regeneration and the preparation method thereof according to claim 1 are characterized in that the blending step of the essence is as follows:

A. adding extracts of centella, aloe, mulberry leaf, chamomile and poria cocos sclerotium into a cosmetic matrix according to parts by weight, and uniformly stirring.

B. And (3) adding the extracted PDRN into the mixed extract A according to the parts by weight, and fully and uniformly mixing to obtain the essence.