CN108866124A - A kind of caper amylorrhexis polysaccharide and its preparation method and application - Google Patents
A kind of caper amylorrhexis polysaccharide and its preparation method and application Download PDFInfo
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- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
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- C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
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- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
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Abstract
The present invention discloses a kind of caper amylorrhexis polysaccharide and preparation method and application, the caper amylorrhexis polysaccharide crushes caper fruit, alkaline hydrogen peroxide processing is added in gained caper powder, it extracts, be then centrifuged for, deionized water is added after collecting gained washing of precipitate, gelatinization, alpha-amylase is added after moisturizing is cooled to 60 DEG C after gelatinization to be digested, gained enzymolysis liquid is added in ethanol water to stand and filter afterwards for 24 hours, gained filter cake is spray-dried to get the caper amylorrhexis polysaccharide that can be used as diminishing moisture-retaining.It is added to during skin applies some make up, final resulting skin care applies some make up natural safety, has good moistening effect.
Description
Technical field
The present invention relates to a kind of caper amylorrhexis polysaccharide and preparation method thereof and its application in skin protection cosmetics.
Technical background
Caper (Capparis spinosa L) is Capparidaceae Chinese lime platymiscium, also known as Capparis Spirrosa, caper, open country
Watermelon is mainly distributed on the ground such as Xinjiang, Gansu and Tibet in China, is mainly distributed on Mediterranean gulf area in foreign countries.Caper
It is Uygur medicine medicinal herbs most in use, the chemical component in caper is mainly sugared and glycoside, flavonoids, alkaloids, terpene and waves
Hair oil, fatty acid and steroid etc..Caper has extensive pharmacological action, including anti-inflammatory analgesic, antifungic action, guarantor
Liver effect, hypoglycemic effect, drop hyperlipidemia effect, antioxidation, blood coagulation resisting function, excited smooth muscle effect, resisting stress are anti-
Answer, improve memory etc..With the internal abnormal humour of removing, swelling and pain relieving, wind-dispelling, cold dispelling, dehumidifying, unimpeded retardance and other effects.Face
Bed is mainly used for the treatment of the bone and joint diseases such as gout, rheumatic arthritis.In Turkey, India and Middle East as one
Kind traditional herbal medicine for nourishing, being anti-inflammatory, anti-hypertension, hypoglycemic, gout, resolving sputum, expelling parasite, diuresis, defaecation etc..
In addition to above-mentioned medical care effect, also using the skin conditioner for preparing natural safety the effect of caper.Mesh
It is preceding there is not yet its related preserving moisture and protecting skin effect and as moisturizer be applied to skin apply some make up in research report.
Summary of the invention
An object of the present invention is to provide a kind of caper amylorrhexis polysaccharide and preparation method thereof, the caper starch
It is completely pure natural, safe to digest polysaccharide, and there is certain anti-oxidant, antibacterial and moistening effect.
The second object of the present invention is to provide above-mentioned caper amylorrhexis polysaccharide as diminishing moisture-retaining in skin care
Application in cosmetic provides a kind of facial treatment milk containing above-mentioned caper amylorrhexis polysaccharide, i.e. moisturizing essence cream.
Technical solution of the present invention
A kind of caper amylorrhexis polysaccharide, method as follows are prepared：
（1）, crush；
（2）, degreasing；
（3）, isolate starch；
（4）, enzymatic starch obtain polysaccharide.
In wherein some embodiments, step（1）Described in crushing be by caper fruit pulverizer control power
For 1100W, frequency is that 50Hz carries out 5~15min of crushing, crosses 80 meshes to get caper powder；
In wherein some embodiments, step（2）Described in degreasing be by step（1）It is double that alkalinity is added in resulting caper powder
Oxygen water process（Alkaline hydrogen peroxidepretreatment, AHPP）, feed liquid mass volume ratio is 1: 20
（g/mL）, it is 2.5% hydrogen peroxide and the mixed solution of 5%NaOH, soak at room temperature 24 that alkaline hydrogen peroxide, which is containing mass fraction,
H, after, control revolving speed is that 2500r/min is centrifuged 10min, collects precipitating；
In wherein some embodiments, step（3）Described in starch of isolating be with clear water rinsing step（2）Resulting precipitating
Until the pH of efflux is neutral, 5-8 times of volumes of deionized water is then added, obtains suspension, it is spare；
In wherein some embodiments, step（4）Described in enzymatic starch to obtain polysaccharide be by step（3）Resulting suspension,
Controlled at 95 DEG C of gelatinization 10-15min, after gelatinization, moisturizing is added alpha-amylase after being cooled to 60 DEG C and carries out enzymatic hydrolysis 3-
Then 4h, preferably 4h calculate enzymolysis liquid after enzymatic hydrolysis, i.e. enzymolysis liquid by volume：The ethanol water that concentration of volume percent is 90%
Solution is 1:Enzymolysis liquid after enzymatic hydrolysis is added in the ethanol water that concentration of volume percent is 90% by 10 ratio, is stood
After 12h, filtering, gained filter cake is spray-dried to get caper amylorrhexis polysaccharide；
The vigor of alpha-amylase used in above-mentioned enzymatic hydrolysis is 40-60U/mg, and dosage presses caper starch：Alpha-amylase is 1g:100
The ratio of~200U calculates.
Wherein, step（2）Described in degreasing be added alkaline hydrogen peroxide processing, on the one hand can play the role of degreasing,
It in favor of subsequent enzymatic hydrolysis, on the other hand can allow starch that can preferably be precipitated out, be then that alkaline hydrogen peroxide also holds repeatedly
It easily removes, does not influence subsequent enzymatic hydrolysis.
A kind of above-mentioned caper amylorrhexis polysaccharide, can due to good skin effect and stronger moisture-retaining capacity
As safely and effectively diminishing moisture-retaining.Using the diminishing moisture-retaining prepare a series of skins apply some make up with moistening effect compared with
Well, naturally safely, Wheat Protein, bacteriostasis, the effect for removing free radical while moisturizing.
A kind of above-mentioned caper amylorrhexis polysaccharide as diminishing moisture-retaining prepare skin apply some make up in application, this
It is only illustrated with it in preparation moisturizing essence cream in invention preferred embodiment, but does not limit it as diminishing moisture-retaining at it
Application in his skin-protection product.
A kind of application of the above-mentioned caper amylorrhexis polysaccharide as diminishing moisture-retaining in cosmetics, caper starch
The dosage for digesting polysaccharide, is calculated by mass percentage, additive amount accounts for the 1-8wt% of cosmetics gross mass.
A kind of moisturizing essence cream containing above-mentioned caper amylorrhexis polysaccharide, is calculated by mass percentage, raw material
Content and composition it is as follows：
Emollient 0.01-1%
Anti-inflammatory agent 1-5%
Moisturizer 1-40%
Thickener 0-1%
Neutralizer 0-1%
Preservative 0.1-0.3%
Above-mentioned caper amylorrhexis polysaccharide 1-8%
Surplus is deionized water.
The emollient include but is not limited to olive oil, macadimia nut oil, Sweet Almond Oil, grape seed oil, avocado oil,
Corn oil, sesame oil, soybean oil, peanut oil, white pond flower seed oil, safflower seed oil, dog rose fruit are oily, thorn A Gan tree benevolence is oily, suddenly
The auspicious palmitic acid fruit oil of bar seed oil, sunflower seed oil, hair, saualane, ethylhexyl palmitate, isopropyl myristate, hydrogenation gather different suddenly
Butylene, isohexadecane, Jojoba esters, dimethyl silicone polymer, Permethyl 99A, diethyl hexylcarbonate, carbonic acid dioctyl ester, two
Isooctadecanol malate, Hamposyl L isopropyl ester, isononyl isononanoate, Nexbase 2004, glycerol three (thylhexoic acid)
Ester, cetanol ethylhexoate, double-diethoxy diethylene glycol (DEG) hexamethylene 1,4- dicarboxylic ester, caprylic/capric triglyceride, oil
Alcohol eruciate, double-PEG-18 methyl ether dimethylsilanes, PPG-10 methyl ether, octyl dodecanol myristinate, hydroxyl
Phenylpropionamide benzoic acid, niacinamide, octyl dodecanol, dimethyl silicone polymer, octyl polymethyl siloxane, Xylitol-based Portugal
One of glucosides, cetyl dimethicone, ring five dimethyl silicone polymer etc. are a variety of.
The moisturizer includes but is not limited to glycerol, anhydro xylitol, diglycerol, propylene glycol, 1,3- propylene glycol, 1,2- penta
Glycol, 1,2- hexylene glycol, dipropylene glycol, polyethylene glycol-8, polyethylene glycol -32, methyl gluceth -10, methyl alcohol
Polyethers -20, PEG/PPG-17/6 copolymer, glycerin polyether -7, glycerin polyether -26, glycosylglycerol, PPG-10 methyl
Ether, PPG-20 methyl ether, -8/5/3 glycerol of PEG/PPG/ polytetramethylene glycol, sucrose, trehalose, rhamnose, mannose, cotton
Sub- sugar, glycine betaine, erythrite, xylitol, biological carbohydrate gum -2, urea, -5 lactate of glycerin polyether, Sodium Hyaluronate, β-Portugal are poly-
Sugar, carnosine, hydrolysis Sodium Hyaluronate, acetylation Sodium Hyaluronate, polyglutamic acid sodium, Sodium Hyaluronate cross-linked polymer, hydrolysis
One of Scleroglucan, budding short stalk enzyme polysaccharide, tremella polysaccharides, tamarind seed polysaccharide, ethoxy urea etc. are a variety of.
The thickener includes but is not limited to Carbomer, acrylic acid (ester) class and its derivative, xanthan gum, Arab
Glue, PVM/MA copolymer, polyethylene glycol -14M, polyethylene glycol -90M, succinoglycan, hydroxyethyl cellulose, acrylic acid（Ester）
The high molecular polymers such as class/C10-30 alkylol acrylamide acid esters cross-linked polymer, hydroxypropyl cellulose, hydroxypropyl methyl cellulose
Deng one of or it is a variety of.
The preservative includes but is not limited to methyl hydroxybenzoate, Nipasol, Phenoxyethanol, benzyl alcohol, benzyl carbinol, bis- (hydroxyls
One of methyl) imidazolidinyl urea, potassium sorbate, sodium benzoate, Chlorphenesin, parahydroxyacet-ophenone, dehydroactic acid sodium etc.
Or it is a variety of.
The neutralizer includes but is not limited to arginine, citric acid, tromethamine, sodium citrate, sodium hydroxide, hydroxide
One of potassium etc. is a variety of.
The anti-inflammatory agent include but is not limited to calendula extract, bisabolol, saccharomycetes to make fermentation Product filtrate, Lu Ding,
Glycyrrhizic acid A, dipotassium glycyrrhizinate, decoyl salicylic acid, palmityl collagen acid, tea tree ethereal oil, azelaic acid derivant, chamomile
One of extract liquor, bisabolol, peony root extractive, Ramulus et Folium Spiraeae Salicifoliae extract, azelaic acid, kushenin etc. are a variety of.
Beneficial effects of the present invention：
A kind of caper amylorrhexis polysaccharide of the invention, since it has the effect of moisturizing, anti-oxidant, antibacterial, this hair
A kind of bright caper amylorrhexis polysaccharide can be used as a kind of diminishing moisture-retaining and apply in skin applies some make up, while moisturizing
Antibacterial and anti-oxidation efficacy can also be had both, and in the technique of preparation caper amylorrhexis polysaccharide, not introducing has human body
Harmful chemical component, it is highly-safe.In addition, a kind of its preparation process simple possible of caper amylorrhexis polysaccharide of the invention,
Suitable for industrialized production.
Specific embodiment
Embodiment 1
A kind of caper amylorrhexis polysaccharide, method as follows are prepared：
（1）, 500g caper fruit is controlled power with pulverizer is 1100W, frequency is that 50Hz carries out crushing 5min, crosses 80 mesh
Sieve is to get caper powder；
（2）, by 400g step（1）It is 2.5% hydrogen peroxide and 5%NaOH that resulting caper powder, which is added 8000mL to contain mass fraction,
Alkaline hydrogen peroxide in, 24 h of soak at room temperature, after, control revolving speed be 2500r/min be centrifuged 10min, collect precipitating；
（3）, with clear water rinsing step（2）Until the resulting pH for being precipitated to efflux is neutral, be then added 5 times of volumes go from
Sub- water obtains suspension, spare；
（4）, by step（3）Resulting suspension, controlled at 95 DEG C of gelatinization 10min, after gelatinization, moisturizing is cooled to 60
Alpha-amylase is added after DEG C and carries out enzymatic hydrolysis 3h, then calculates enzymolysis liquid after enzymatic hydrolysis by volume, i.e. enzymolysis liquid：Percent by volume
The ethanol water that concentration is 90% is 1:Enzymolysis liquid after enzymatic hydrolysis is added to the second that concentration of volume percent is 90% by 10 ratio
In alcohol solution, after standing 12h, filtering, gained filter cake is spray-dried to get caper amylorrhexis polysaccharide 316.4g；
The vigor of alpha-amylase used in above-mentioned enzymatic hydrolysis is 40-60U/mg, and dosage presses caper starch：Alpha-amylase is 1g:100
The ratio of~200U calculates.
Embodiment 2
A kind of caper amylorrhexis polysaccharide, method as follows are prepared：
（1）, 500g caper fruit is controlled power with pulverizer is 1100W, frequency is that 50Hz carries out crushing 15min, crosses 80
Mesh is to get caper powder；
（2）, by 400g step（1）It is 2.5% hydrogen peroxide and 5%NaOH that resulting caper powder, which is added 8000mL to contain mass fraction,
Alkaline hydrogen peroxide in, 24 h of soak at room temperature, after, control revolving speed be 2500r/min be centrifuged 10min, collect precipitating；
（3）, with clear water rinsing step（2）Until the resulting pH for being precipitated to efflux is neutral, be then added 8 times of volumes go from
Sub- water obtains suspension, spare；
（4）, by step（3）Resulting suspension, controlled at 95 DEG C of gelatinization 15min, after gelatinization, moisturizing is cooled to 60
Alpha-amylase is added after DEG C and carries out enzymatic hydrolysis 4h, then calculates enzymolysis liquid after enzymatic hydrolysis by volume, i.e. enzymolysis liquid：Percent by volume
The ethanol water that concentration is 90% is 1:Enzymolysis liquid after enzymatic hydrolysis is added to the second that concentration of volume percent is 90% by 10 ratio
In alcohol solution, after standing 12h, filtering, gained filter cake is spray-dried to get caper amylorrhexis polysaccharide 326.7g；
The vigor of alpha-amylase used in above-mentioned enzymatic hydrolysis is 40-60U/mg, and dosage presses caper starch：Alpha-amylase is 1g:100
The ratio of~200U calculates.
Embodiment 3
A kind of caper amylorrhexis polysaccharide, method as follows are prepared：
（1）, 500g caper fruit is controlled power with pulverizer is 1100W, frequency is that 50Hz carries out crushing 10min, crosses 80
Mesh is to get caper powder；
（2）, by 400g step（1）It is 2.5% hydrogen peroxide and 5%NaOH that resulting caper powder, which is added 8000mL to contain mass fraction,
Alkaline hydrogen peroxide in, 24 h of soak at room temperature, after, control revolving speed be 2500r/min be centrifuged 10min, collect precipitating；
（3）, with clear water rinsing step（2）Until the resulting pH for being precipitated to efflux is neutral, 6.5 times of volumes are then added and go
Ionized water obtains suspension, spare；
（4）, by step（3）Resulting suspension, controlled at 95 DEG C of gelatinization 12min, after gelatinization, moisturizing is cooled to 60
Alpha-amylase is added after DEG C and carries out enzymatic hydrolysis 4h, then calculates enzymolysis liquid after enzymatic hydrolysis by volume, i.e. enzymolysis liquid：Percent by volume
The ethanol water that concentration is 90% is 1:Enzymolysis liquid after enzymatic hydrolysis is added to the second that concentration of volume percent is 90% by 10 ratio
In alcohol solution, after standing 12h, filtering, gained filter cake is spray-dried to get caper amylorrhexis polysaccharide 319.8g；
The vigor of alpha-amylase used in above-mentioned enzymatic hydrolysis is 40-60U/mg, and dosage presses caper starch：Alpha-amylase is 1g:100
The ratio of~200U calculates.
Comparative example 1
（1）, 500g caper fruit is controlled power with pulverizer is 1100W, frequency is that 50Hz carries out crushing 10min, crosses 80
Mesh is to get caper powder；
（2）, by 400g step（1）Resulting caper powder is added 8000mL and contains in the solution that mass fraction is 5%NaOH, often
Temperature impregnates 24 h, after, control revolving speed is that 2500r/min is centrifuged 10min, collects precipitating；
（3）, with clear water rinsing step（2）Until the resulting pH for being precipitated to efflux is neutral, 6.5 times of volumes are then added and go
Ionized water obtains suspension, spare；
（4）, by step（3）Resulting suspension, controlled at 95 DEG C of gelatinization 12min, after gelatinization, moisturizing is cooled to 60
Alpha-amylase is added after DEG C and carries out enzymatic hydrolysis 4h, then calculates enzymolysis liquid after enzymatic hydrolysis by volume, i.e. enzymolysis liquid：Percent by volume
The ethanol water that concentration is 90% is 1:Enzymolysis liquid after enzymatic hydrolysis is added to the second that concentration of volume percent is 90% by 10 ratio
In alcohol solution, after standing 12h, filtering, gained filter cake is spray-dried to get caper amylorrhexis polysaccharide 216.6g；
The vigor of alpha-amylase used in above-mentioned enzymatic hydrolysis is 40-60U/mg, and dosage presses caper starch：Alpha-amylase is 1g:100
The ratio of~200U calculates.
Comparative example 2
A kind of caper amylorrhexis polysaccharide, method as follows are prepared：
（1）, 500g caper fruit is controlled power with pulverizer is 1100W, frequency is that 50Hz carries out crushing 10min, crosses 80
Mesh is to get caper powder；
（2）, by 400g step（1）Resulting caper powder, which is added 8000mL and contains mass fraction, is in 2.5% hydrogen peroxide solution,
24 h of soak at room temperature, after, control revolving speed is that 2500r/min is centrifuged 10min, collects precipitating；
（3）, with clear water rinsing step（2）Until the resulting pH for being precipitated to efflux is neutral, 6.5 times of volumes are then added and go
Ionized water obtains suspension, spare；
（4）, by step（3）Resulting suspension, controlled at 95 DEG C of gelatinization 12min, after gelatinization, moisturizing is cooled to 60
Alpha-amylase is added after DEG C and carries out enzymatic hydrolysis 4h, then calculates enzymolysis liquid after enzymatic hydrolysis by volume, i.e. enzymolysis liquid：Percent by volume
The ethanol water that concentration is 90% is 1:Enzymolysis liquid after enzymatic hydrolysis is added to the second that concentration of volume percent is 90% by 10 ratio
In alcohol solution, after standing 12h, filtering, gained filter cake is spray-dried to get caper amylorrhexis polysaccharide 183.4g；
The vigor of alpha-amylase used in above-mentioned enzymatic hydrolysis is 40-60U/mg, and dosage presses caper starch：Alpha-amylase is 1g:100
The ratio of~200U calculates.
Comparative example 3
A kind of caper amylorrhexis polysaccharide, method as follows are prepared：
（1）, 500g caper fruit is controlled power with pulverizer is 1100W, frequency is that 50Hz carries out crushing 10min, crosses 80
Mesh is to get caper powder；
（2）, by 400g step（1）Resulting caper powder is added in 8000mL water, 24 h of soak at room temperature, after, control turns
Speed is that 2500r/min is centrifuged 10min, collects precipitating；
（3）, with clear water rinsing step（2）Until the resulting pH for being precipitated to efflux is neutral, 6.5 times of volumes are then added and go
Ionized water obtains suspension, spare；
（4）, by step（3）Resulting suspension, controlled at 95 DEG C of gelatinization 12min, after gelatinization, moisturizing is cooled to 60
Alpha-amylase is added after DEG C and carries out enzymatic hydrolysis 4h, then calculates enzymolysis liquid after enzymatic hydrolysis by volume, i.e. enzymolysis liquid：Percent by volume
The ethanol water that concentration is 90% is 1:Enzymolysis liquid after enzymatic hydrolysis is added to the second that concentration of volume percent is 90% by 10 ratio
In alcohol solution, after standing 12h, filtering, gained filter cake is spray-dried to get caper amylorrhexis polysaccharide 166.2g；
The vigor of alpha-amylase used in above-mentioned enzymatic hydrolysis is 40-60U/mg, and dosage presses caper starch：Alpha-amylase is 1g:100
The ratio of~200U calculates.
Test 1 test through transepidermal water evaporation capacity
Caper amylorrhexis polysaccharide obtained by embodiment 1-3 and comparative example 1-3 dilutes 100 times with deionized water, gained water
Solution smears skin, and in the 2nd day morning, use water evaporation quantity measurement device (model " バ Port ス キ ャ Application after smearing
AS-VT100RS ", ア サ ヒ テ Network ノ ラ ボ society system), measure each aqueous solution smears position and without smearing position through epidermis
Water evaporation quantity (TEWL value).The measurement result of TEWL value is shown in table 1.
Table 1
1% aqueous solution of caper amylorrhexis polysaccharide obtained by embodiment 1-3 smears TEWL value 6.8-7.2 and the comparison at position
1% aqueous solution of caper amylorrhexis polysaccharide obtained by example 1-3 smears the TEWL value 8.9-9.6 at position and without smearing position
TEWL value 10.4 compare, specify reduce TEWL value, improve skin barrier function, illustrate that caper amylorrhexis of the present invention is more
Sugar is applied in moisturizer, can mention the barrier function of skin；And by embodiment 1-3 and comparative example 1-3 comparison it is found that through
The amylorrhexis polysaccharide that alkaline hydrogen peroxide is handled more preferably, illustrates at alkaline hydrogen peroxide through transepidermal water evaporation capacity (TEWL value)
Reason is committed step.
Test the water absorbing force of 2 skins and the test of moisture holding capacity
Caper amylorrhexis polysaccharide obtained by embodiment 1-3 and comparative example 1-3 dilutes 100 times with deionized water, gained water
Solution smears skin, the 2nd day afternoon after smearing, carries out water load test, every group of 30 people, measures embodiment 1-3 and right
1% aqueous solution of caper amylorrhexis polysaccharide obtained by ratio 1-3 smears position and water absorbing force and water conservation without smearing position
Power.
The evaluation assessment that water load test refers to the water retaining function as cuticula uses the cuticlar water load of the field upper class
Test method(s) carries out (Tagami, H., et al., J.Invest.
Dermatol.78,425-428,1982).Firstly, using epidermis moisture amount determining device (model " Skicon-200 ", ア
イ ビ イ エ ス Co. Ltd. system), the cuticlar water component of recipient site is measured, as blank value.Then, by
The flannelette for trying position mounting 2cm × 2cm, is added dropwise 500 μ L of distilled water, makes skin loadings water 10 seconds.Then flannelette is removed, is used immediately
The moisture of dust-free paper removing skin surface.After flannelette removes, every 30 seconds measurement cuticlar water components until 2 minutes.
For each examinee, the numerical value after flannelette is removed 30 seconds is set as water absorbing force (phase divided by value obtained by blank value
To value), the numerical value after 60,90,120 seconds is set as moisture holding capacity (relative value) divided by value obtained by blank value.Show the result in table 2
In.
Table 2
1% aqueous solution of caper amylorrhexis polysaccharide obtained by embodiment 1-3 and caper starch obtained by comparative example 1-3
It digests 1% aqueous solution of polysaccharide and is compared without smearing, confirm the water absorbing force and moisture holding capacity for improving skin, specify promotion skin
The water tariff collection function of skin.
Experiment shows that caper amylorrhexis polysaccharide of the present invention is applied in moisturizing cosmetic, can promote the moisture of skin
Moisture-keeping functions；And by embodiment 1-3 and comparative example 1-3 comparison it is found that the amylorrhexis polysaccharide handled through alkaline hydrogen peroxide
Water absorbing force and moisture holding capacity are all more preferable, illustrate that alkaline hydrogen peroxide processing is committed step.
Embodiment 4
Prepare moisturizing the essence cream 1-3 and comparative example 4-7 formed as shown in table 3, the component including following mass percentage：
Table 3
It is on probation to test 3 crowds
Moistening effect test
Test sample：Moisturizing essence cream 1-3 and comparative example 4-7 obtained by embodiment 4
Test crowd：20-40 years old 350 people are selected, men and women is fifty-fifty, health, rule of life, and no skin disease is randomly divided into 7
Group, every group of 50 people.
Test method：After study subject cleans facial skin, dries, sample is made in 2g with hand and is applied to facial skin
On, it is then 20 DEG C in test environment temperature, the moisture content of left face is measured in the environment that relative humidity is 60%.It is left on the face
The square Experimental Area for marking 2 2cm × 2cm, as the test section of sample, using Multi-functional skin tester
Corneometer CM825 probe carries out moisture content of skin test, which is to reflect skin by measuring the capacitance of skin
The variation of moisture, CM value is higher, and to represent water content of stratum corneum higher.Multi-functional skin tester measures smearing respectively and sample is made
Facial skin moisture content that is preceding and smearing 2,4 hours after obtained sample, records result.Concrete outcome is shown in Table 4.After unite
Credit analysis is counted, each group comparison result shows P < 0.05, and difference has statistical significance.
Table 4：Moistening effect test result table
Test result shows that moisturizing essence cream 1-3 has significant moistening effect to skin（Relatively initial CM value, uses CM after 2 hours
Value is increased between 62.4-66.7, and after 4 hours, CM value is between 59.2-63.4）, relative contrast example 4-6 have significantly to skin
Moistening effect（Relatively initial CM value, is increased between 44.3-50.3 using CM value after 2 hours, and after 4 hours, CM value is in 35.6-
Between 40.3）, and the comparative example 7 of caper amylorrhexis polysaccharide is not added（40.2 are increased to using CM value after 2 hours, 4 is small
Shi Hou, CM value are 28.6）, moistening effect is also more preferable.Extension at any time, moisture it is lost also slower.And by embodiment 1-3 and
It is found that the amylorrhexis polysaccharide handled through alkaline hydrogen peroxide is added in moisturizing essence cream, moisturizing is imitated for comparative example 4-6 comparison
Fruit is more preferable.
By embodiment 1-3 and the comparison of comparative example 7 it is found that caper amylorrhexis polysaccharide of the present invention is the guarantor of moisturizing essence cream
Wet effect key component, to moistening effect is promoted, effect is significant.
It is reacted according to subject, it is safe and non-stimulating using moisturizing essence cream of the present invention, it has no adverse reaction.
Embodiment described above only describe the preferred embodiments of the invention, not to model of the invention
It encloses and is defined, without departing from the spirit of the design of the present invention, those of ordinary skill in the art are to technical side of the invention
The various changes and improvements that case is made should all be fallen into the protection scope that claims of the present invention determines.
Claims (8)
1. a kind of caper amylorrhexis polysaccharide, which is characterized in that method as follows is prepared：
（1）, crush；
（2）, degreasing；
（3）, isolate starch；
（4）, enzymatic starch obtain polysaccharide.
2. caper amylorrhexis polysaccharide according to claim 1, which is characterized in that step（1）The crushing is will
It is 1100W that caper fruit, which controls power with pulverizer, and frequency is that 50Hz carries out 5~15min of crushing, crosses 80 meshes to get thorn
Chinese lime powder.
3. caper amylorrhexis polysaccharide according to claim 1, which is characterized in that step（2）The degreasing is will
Step（1）Alkaline hydrogen peroxide processing is added in resulting caper powder（alkaline hydrogen
Peroxidepretreatment, AHPP）, feed liquid mass volume ratio is 1: 20（g/mL）, alkaline hydrogen peroxide is to contain quality
Mixed solution of the score for 2.5% hydrogen peroxide and 5%NaOH, 24 h of soak at room temperature, after, control revolving speed is 2500r/min
It is centrifuged 10min, collects precipitating.
4. caper amylorrhexis polysaccharide according to claim 1, which is characterized in that step（3）Described isolates shallow lake
Powder is with clear water rinsing step（2）Until the resulting pH for being precipitated to efflux is neutral, 5-8 times of volume deionization is then added
Water obtains suspension, spare.
5. caper amylorrhexis polysaccharide according to claim 1, which is characterized in that step（4）The enzymatic starch
Obtaining polysaccharide is by step（3）Resulting suspension, controlled at 95 DEG C of gelatinization 10-15min, after gelatinization, moisturizing cooling
Alpha-amylase is added after to 60 DEG C and carries out enzymatic hydrolysis 3-4h, preferably 4h, enzymolysis liquid after enzymatic hydrolysis is calculated by volume then, that is, is digested
Liquid：The ethanol water that concentration of volume percent is 90% is 1:Enzymolysis liquid after enzymatic hydrolysis is added to percent by volume by 10 ratio
In the ethanol water that concentration is 90%, after standing 12h, filtering, gained filter cake is spray-dried more to get caper amylorrhexis
Sugar；The vigor of alpha-amylase used in above-mentioned enzymatic hydrolysis is 40-60U/mg, and dosage presses caper starch：Alpha-amylase is 1g:100
The ratio of~200U calculates.
6. caper amylorrhexis polysaccharide as claimed in claim 1 or 2 is being prepared during skin applies some make up as diminishing moisture-retaining
Application, which is characterized in that the dosage of caper amylorrhexis polysaccharide is calculated by mass percentage, additive amount accounts for cosmetics
The 1-8wt% of gross mass.
7. application according to claim 5, which is characterized in that the skin applies some make up as moisturizing essence cream.
8. a kind of moisturizing essence cream containing caper amylorrhexis polysaccharide as claimed in claim 1 or 2, which is characterized in that
It is calculated by mass percentage, the content and composition of raw material are as follows：
Emollient 0.01-1%
Anti-inflammatory agent 1-5%
Moisturizer 1-40%
Thickener 0-1%
Neutralizer 0-1%
Preservative 0.1-0.3%
Caper amylorrhexis polysaccharide 1-8% as claimed in claim 1 or 2
Surplus is deionized water.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111067842A (en) * | 2020-01-17 | 2020-04-28 | 湖南科技大学 | Preparation method of aluminum oxide-based skin-moistening mask |
| CN111690603A (en) * | 2020-06-22 | 2020-09-22 | 青岛思拓新源细胞医学有限公司 | Biological agent for promoting proliferation and migration of dental pulp stem cells |
| CN114259438A (en) * | 2021-12-21 | 2022-04-01 | 上海珈凯生物科技有限公司 | Capparis spinosa fruit composite liquid and preparation method and application thereof |
-
2018
- 2018-08-06 CN CN201810887732.5A patent/CN108866124A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111067842A (en) * | 2020-01-17 | 2020-04-28 | 湖南科技大学 | Preparation method of aluminum oxide-based skin-moistening mask |
| CN111690603A (en) * | 2020-06-22 | 2020-09-22 | 青岛思拓新源细胞医学有限公司 | Biological agent for promoting proliferation and migration of dental pulp stem cells |
| CN114259438A (en) * | 2021-12-21 | 2022-04-01 | 上海珈凯生物科技有限公司 | Capparis spinosa fruit composite liquid and preparation method and application thereof |
| CN114259438B (en) * | 2021-12-21 | 2023-11-14 | 上海珈凯生物股份有限公司 | Caper fruit compound liquid and preparation method and application thereof |
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Application publication date: 20181123 |