CN114028456A

CN114028456A - Composition for resisting heat stress of young rabbits, application of composition and method for resisting heat stress of young rabbits

Info

Publication number: CN114028456A
Application number: CN202111470827.5A
Authority: CN
Inventors: 任永军; 谢晓红; 季杨; 雷岷; 黄永军; 龙泽呷; 王玉霞; 郭志强; 唐燕红; 邝良德; 郑洁; 李丛艳; 张翠霞; 张翔宇; 杨超; 袁定胜; 唐丽; 杨锐; 李钰莹
Original assignee: Sichuan Animal Science Academy
Current assignee: Sichuan Animal Science Academy
Priority date: 2021-12-03
Filing date: 2021-12-03
Publication date: 2022-02-11
Anticipated expiration: 2041-12-03
Also published as: CN114028456B

Abstract

The invention discloses a composition for resisting heat stress of young rabbits, application thereof and a method for resisting heat stress of young rabbits, wherein the composition for resisting heat stress of young rabbits comprises the following components: bupleuri radix, radix Puerariae, rhizoma Fagopyri Dibotryis and Borneolum Syntheticum; the mass ratio of the bupleurum, the kudzuvine root, the wild buckwheat rhizome and the borneol is 200-. The invention also discloses the application of the composition for resisting heat stress of young rabbits in young rabbit breeding and heat stress resistant feeds for young rabbits, and a method for resisting heat stress of young rabbits. The invention can improve the feed intake of young rabbits, improve the feed conversion rate of the young rabbits, improve the daily gain, improve the production performance such as the survival rate and the like, improve the activity of physiological and biochemical indexes such as superoxide dismutase (SOD) and the like of the young rabbits, and improve the drug resistance and the heat stress resistance of the young rabbits.

Description

Composition for resisting heat stress of young rabbits, application of composition and method for resisting heat stress of young rabbits

Technical Field

The invention relates to the technical field of heat stress resistance of young rabbits, in particular to a composition for resisting heat stress of young rabbits, application thereof and a method for resisting heat stress of young rabbits.

Background

The high temperature and high humidity environment in summer can cause the heat stress reaction of animals, and has great influence on animal husbandry. The heat stress reaction can cause the reduction of the production performance and the reproductive performance of the rabbits, wherein the young rabbits mainly show that the feed intake is reduced, the feed conversion rate is reduced, the daily gain is reduced, the death rate is obviously increased, and huge economic loss is brought to the breeding production of the rabbits. The influence of heat stress on the productivity of young rabbits may be caused by the fact that the feed intake of the rabbits is reduced in a high-temperature environment, and metabolic disorders and an increase in respiratory frequency lose more energy, resulting in a reduction in metabolic energy for growth.

The heat stress reaction of the meat rabbits is easily induced in the high-temperature and high-humidity environment in summer, and the physiological and biochemical indexes of the young rabbits are as follows except that the apparent feeding behavior, the production performance and the like are reduced: serum levels of triiodothyronine (T3), thyroxine (T4) were reduced, cortisol levels, creatine kinase activity and white blood cell count, neutrophil percentage, neutrophil to lymphocyte ratio were increased.

The heat stress obviously reduces the activity of Glutathione (GSH), Catalase (CAT) and superoxide dismutase (SOD) in the serum of young rabbits. Research shows that when the young rabbit is fed with the young rabbit 21d in a high-temperature (30-37 ℃ and 65% humidity) environment, the total antioxidant capacity (T-AOC) and SOD activity of liver tissues are obviously reduced, and the content of Malondialdehyde (MDA) is increased. In the early stage of heat stress, endogenous SOD in the body increases and removes oxygen free radicals and reduces the generation of lipid peroxidation products, and long-time heat stress causes the oxygen free radicals in the body to continuously increase and consume excessive antioxidant enzymes, thus leading to the reduction of SOD level.

Disclosure of Invention

Aiming at the defects in the prior art, the invention provides a composition for resisting heat stress of young rabbits, application thereof and a method for resisting heat stress of young rabbits, which improve the feed intake of young rabbits, improve the feed conversion rate of the young rabbits, improve the daily gain, improve the production performance such as the survival rate and the like, improve the activity of physiological and biochemical indexes such as superoxide dismutase (SOD) and the like of the young rabbits, and improve the drug resistance and the heat stress resistance of the young rabbits.

A composition for resisting heat stress of young rabbits comprises the following components: bupleuri radix, radix Puerariae, rhizoma Fagopyri Dibotryis and Borneolum Syntheticum;

the mass ratio of the bupleurum, the kudzuvine root, the wild buckwheat rhizome and the borneol is 200-.

Further, the mass ratio of the bupleurum, the kudzuvine root, the wild buckwheat and the borneol is 400: 150-.

Further, the mass ratio of the bupleurum, the kudzuvine root, the wild buckwheat rhizome and the borneol is 300:200:300: 200.

Furthermore, the mesh number of the bupleurum, the kudzuvine root, the wild buckwheat and the borneol is 200-400 meshes.

Bupleuri radix has the effects of harmonizing exterior and interior, soothing liver and invigorating yang, and is commonly used for treating common cold, fever, alternating chills and fever, stagnation of qi due to depression of the liver, distending pain in chest and hypochondrium, convulsion and the like. The bupleuri radix mainly comprises bupleuri radix saponin, volatile oil (eugenol, etc.), polysaccharide, flavone, sterol, etc.; the saikoside has strong enzyme secretion promoting effect, can induce liver enzyme activity, and improve liver superoxide dismutase (SOD) activity, and has the effect of improving peristalsis of rabbit isolated intestinal canal, which is not resisted by atropine, and can improve rabbit feed intake and daily gain. Radix bupleuri can also significantly reduce serum total cholesterol, triglyceride and low density lipoprotein cholesterol of young rabbit.

The kudzu root is sweet and pungent in taste and cool in nature, enters lung and stomach channels, and has the effects of relieving muscles and reducing fever, promoting eruption, promoting the production of body fluid to quench thirst, invigorating yang and relieving diarrhea and the like. The main active ingredient of the kudzuvine root is isoflavone compound puerarin which has the functions of resisting oxidation damage, resisting apoptosis, improving blood fat and blood sugar metabolism and the like. Modern pharmacological research shows that the kudzuvine root has the effects of clearing heat and relieving summer heat, and the pueraria flavone is an effective natural free radical scavenger, has strong reducing capability and has a certain scavenging effect on superoxide anion free radicals, OH and Diphenyl Picryl Phenylhydrazine (DPPH). When an animal is in disorder of energy metabolism under the condition of heat stress, blood supply and oxygen supply are recovered at the moment, a large amount of free radicals are generated instantly and exceed the loadable capability of an organism, so that peroxidation damage is caused, protein and DNA are damaged, cells are killed, and the free radical scavenging capability of pueraria flavone plays a role basis of DNA protection. The puerarin has unique activity in improving the hypoxia tolerance of animals, and has the function of remarkably prolonging the survival time of tissue hypoxia caused by the heat stress of the animals.

The wild buckwheat rhizome is pungent, bitter and cool in nature, enters lung meridian, has the effects of clearing heat and removing toxicity, eliminating carbuncle and expelling pus, tonifying spleen and removing dampness, and activating blood and treating sore, and is mainly used for treating lung carbuncle, lung heat cough and asthma, sore throat, dysentery, rheumatism and arthralgia syndrome, traumatic injury, carbuncle and cancer swelling and the like. The main components of the composition comprise flavonoids, terpenoids, enzymes, volatile components and the like. Modern pharmacological studies show that the active ingredients of the wild buckwheat rhizome have the effects of resisting oxidation, enhancing immunologic function, resisting bacteria, diminishing inflammation and the like, wherein the level of superoxide dismutase (SOD) in animal serum can be obviously improved, meanwhile, intestinal mucositis is reduced, intestinal mucosal barrier is improved, and the digestion and absorption of intestinal tracts to nutrient substances are facilitated.

Borneol is pungent and bitter, is slightly cold and cool in nature, and has the effects of refreshing, restoring consciousness, inducing resuscitation with aromatics and the like. The main component of borneol is d-borneol, and at the same time contains several sesquiterpenes including humulene and caryophyllene, and its effective component has the functions of resisting bacteria and fungi, relieving inflammation and stopping pain, etc. Borneol is commonly used as an adjuvant in the compatibility of traditional Chinese medicines, namely, the borneol helps other medicines to better exert curative effect, and the modern pharmacology and pharmacodynamics show that the borneol helps other medicines to permeate blood brain barrier and exert the medicinal effect.

Modern medical research shows that the effective components of the bupleurum have the efficacy of removing bacterial endotoxin, have good antipyretic effect on rabbits with bacterial endotoxin pyrogenicity, and have the antipyretic effect equivalent to aspirin when being used for a long time in large dose. The wild buckwheat rhizome extract can obviously inhibit the activity of extracellular heat-resistant nuclease of staphylococcus aureus; in vitro test research shows that the wild buckwheat rhizome extract has an inhibiting effect on salmonella, and the like, shows a good protection effect on test animals when mice and rabbits are artificially infected with more than 10 pathogenic bacteria such as staphylococcus, streptococcus, salmonella, and the like in vivo, and simultaneously finds that the wild buckwheat rhizome extract has an immunopotentiation effect of increasing leucocytes and protecting neutrophils. The in vitro antibacterial activity of different formulations of borneol is measured to obtain the borneol antibacterial composition which has stronger antibacterial action on pathogenic microorganisms such as staphylococcus aureus, fungi and the like.

The composition for resisting heat stress of young rabbits is applied to breeding of young rabbits.

The composition for resisting heat stress of young rabbits is applied to heat stress resistant feed for young rabbits.

A young rabbit feed comprises the composition for resisting heat stress of young rabbits.

A method for resisting heat stress of young rabbits comprises adding the composition for resisting heat stress of young rabbits into daily ration of young rabbits at an addition amount of 600-1500g/1000kg, and feeding for more than 10-14 days.

Further, the amount of addition was 1000g/1000 kg.

In summary, the invention has the following advantages:

1. the composition for resisting heat stress of the young rabbits can improve the feed intake of the young rabbits, improve the feed conversion rate of the young rabbits, improve the daily gain, improve the production performance such as the survival rate and the like, improve the activity of physiological and biochemical indexes such as superoxide dismutase (SOD) and the like of the young rabbits, and improve the drug resistance and the heat stress resistance of the young rabbits. .

2. The composition for resisting heat stress of the young rabbits can improve the feed intake of the young rabbits, improve the feed conversion rate of the young rabbits, improve the daily gain, improve the production performance such as the survival rate and the like, improve the activity of physiological and biochemical indexes such as superoxide dismutase (SOD) and the like of the young rabbits, and improve the heat medicine property and the heat stress resistance of the young rabbits. The anti-heat stress prescription comprises: radix bupleuri is good in dispelling and relieving fever, radix puerariae is good in expelling muscles and relieving fever, the two medicines are bitter, pungent and cold in flavor, and have the effects of clearing heat and purging fire, detoxifying and resolving masses when used together, and the radix bupleuri and the radix puerariae are monarch medicines together; the wild buckwheat rhizome is a ministerial drug which has the effects of clearing heat and removing toxicity, promoting digestion and removing food stagnation, cooling blood and resolving carbuncle, has stronger effects of clearing heat and removing toxicity and cooling blood; borneol is an adjuvant drug for inducing resuscitation and refreshing mind and clearing away the fire of the lung and stomach, so that the heat and toxicity inside the body can be cleared away; the medicines have the effects of clearing heat and cooling blood, promoting digestion and resolving stagnation, effectively improve the heat stress of the rabbits and improve the production performance.

3. From modern pharmacology, the saikoside has extremely strong enzyme secretion promoting effect, improves the activity of liver superoxide dismutase (SOD), has the function of enhancing peristalsis on rabbit intestinal tracts, and can improve the food intake of rabbits; when the young rabbits are in the heat stress environment and have energy metabolism disorder, the organism can generate a large amount of free radicals in order to recover blood supply and oxygen supply and exceed the loadable capacity of the organism, so that peroxidation damage is caused, protein and DNA are damaged and cells die, pueraria flavone can clear the free radicals, the cells of the organism are protected to play DNA efficacy, puerarin components can improve the hypoxia tolerance of the young rabbits, and the survival time of the young rabbits is remarkably prolonged for tissue hypoxia caused by the young rabbits in the heat stress environment, so that the survival rate of the young rabbits in the heat stress environment is improved; the wild buckwheat can remarkably improve the level of superoxide dismutase (SOD) in animal serum, simultaneously reduce intestinal mucositis, improve intestinal mucosal barrier and facilitate the digestion and absorption of nutrient substances by intestinal tracts; the borneol is helpful for other medicines to penetrate through the blood brain barrier and exert the drug effect. Meanwhile, the radix bupleuri, the wild buckwheat rhizome and the borneol have good inhibition effect on pathogenic microorganisms such as bacteria and the like, have protection effect on preventing and treating infection of pathogenic microorganisms such as bacteria and the like inside and outside the young rabbit body, and can improve death caused by bacterial diseases of the young rabbit and improve survival rate.

4. The composition has the advantages of remarkable effect, simple operation and low cost. Through scientific research and demonstration, the method has good academic value and market application prospect. The invention discovers that: the composition added into the daily ration of young rabbits can improve the activity of liver superoxide dismutase (SOD) and other physiological and biochemical indexes of the young rabbits, reduce the rectal temperature of the young rabbits, and improve the heat stress resistance (heat resistance) of the young rabbits; in the aspect of production performance, the feed intake, feed conversion rate, daily gain and survival rate of young rabbits can be obviously improved. In conclusion, the composition consisting of the radix bupleuri, the radix puerariae, the wild buckwheat rhizome and the borneol can obviously improve the heat stress resistance of young rabbits, and has good academic value and wide market application prospect.

Detailed Description

Example 1

A composition for resisting heat stress of young rabbits comprises the following components: bupleuri radix, radix Puerariae, rhizoma Fagopyri Dibotryis and Borneolum Syntheticum; the mass ratio of the bupleurum, the kudzuvine root, the wild buckwheat rhizome and the borneol is 200:100:200: 100. The mesh number of the bupleurum, the kudzuvine root, the wild buckwheat rhizome and the borneol is 200 meshes.

The composition for resisting the heat stress of the young rabbits is used for breeding the young rabbits and is applied to heat stress resistant feeds for the young rabbits.

A method for resisting heat stress of young rabbits comprises adding the composition for resisting heat stress of young rabbits into daily ration of young rabbits at an addition amount of 600g/1000kg, and feeding for more than 12 days.

Example 2

A composition for resisting heat stress of young rabbits comprises the following components: bupleuri radix, radix Puerariae, rhizoma Fagopyri Dibotryis and Borneolum Syntheticum; the mass ratio of the bupleurum, the kudzuvine root, the wild buckwheat rhizome and the borneol is 300:200:300: 200. The mesh number of the bupleurum, the kudzuvine root, the wild buckwheat rhizome and the borneol is 300 meshes.

A method for resisting heat stress of young rabbits comprises adding the composition for resisting heat stress of young rabbits into daily ration of young rabbits at an addition amount of 1000g/1000kg, and feeding for 14 days or more.

Example 3

A composition for resisting heat stress of young rabbits comprises the following components: bupleuri radix, radix Puerariae, rhizoma Fagopyri Dibotryis and Borneolum Syntheticum; the mass ratio of the bupleurum, the kudzuvine root, the wild buckwheat rhizome and the borneol is 500:300:400: 300. The mesh number of the bupleurum, the kudzuvine root, the wild buckwheat rhizome and the borneol is 400 meshes.

A method for resisting heat stress of young rabbits comprises adding the composition for resisting heat stress of young rabbits at an addition amount of 1500g/1000kg into daily ration of young rabbits for more than 14 days.

Experimental example 1

200 healthy 35-day-old weaned New Zealand rabbits are selected, randomly divided into 4 groups of 50 rabbits each and fed in a single cage, each group is 5 replicates, 10 replicates are respectively corresponding to a test 1 group, a test 2 group, a test 3 group and a control group (the test design is shown in table 1), the test starting time is 35 days old, the test period is 6 weeks, and the test is ended at 77 days old. The composition and nutritional level of the diet are shown in table 2. The test is carried out under the control test condition, all the test animals are raised in the same rabbit hutch, and the average temperature-humidity index (THI) of the rabbit hutch per day is controlled to be between 28.9 and 30.0, so that the test animals are in the severe heat stress environment. The test animals adopt free feeding and automatic drinking. Other feeding management and immunization programs were performed as normal procedures.

300g of radix bupleuri, 200g of radix puerariae, 300g of wild buckwheat rhizome and 200g of borneol in the composition for resisting heat stress of young rabbits are screened by a 300-mesh sieve and uniformly mixed for later use.

TABLE 1 design of the experiments

TABLE 2 basic diet composition and Nutrition level (air-dried basis)

Note: 1) the premix can provide per kilogram of complete feed: 120Mg of Cu, 90Mg of Zn, 30Mg of Mn, 150Mg of Mg, 4000IU of VA, 31000 IU of VD, 50Mg of VE and 1Mg of choline.

2) Digestion energy was calculated, and the rest were measured.

The rectal temperature of the test animals in the test groups 1-4 is measured at 35 days, 49 days, 63 days and 77 days, the weight is weighed, the survival rate is counted, the average daily feed intake, the average daily gain and the feed conversion rate are calculated, 5ml of blood is collected from the ear artery at the time point, serum is separated, and the superoxide dismutase (SOD), the Catalase (CAT), the glutathione peroxidase (GSH), the triiodothyronine (T3) and the thyroxine (T4) are measured by a full-automatic biochemical analyzer.

Statistical analysis: after the test data are processed by Excel2013 software, SPSS16.0 statistical software is adopted for variance analysis, multiple comparisons are carried out by a Duncan method, the result is expressed by 'mean value plus or minus standard error', and P <0.05 is used as a difference significance judgment standard.

1. The effect of heat stress environment on rectal temperature of young rabbits is shown in table 3.

TABLE 3 Effect of Heat stress Environment on rectal temperature of Young rabbits

Note: the data in the same column are marked with different lower case letters to indicate significant difference (P <0.05), and the same or no letters to indicate insignificant difference (P > 0.05).

Rectal temperature: the age of 35 days, the test 1, the test 23, the test group and the control group are respectively 38.23 ℃, 38.41 ℃, 38.37 ℃ and 738.32 ℃, and the significance among the test groups is not significant (P is more than 0.05); 49 days old, the test groups 1, 2 and 3 and the control group are respectively 40.87 ℃, 40.32 ℃, 39.98 ℃ and 42.38 ℃, the test group 3 is obviously lower than the test group 1 and the control group (P is less than 0.05), the control group is obviously higher than the test groups 1, 2 and 3, the difference between the test groups 1 and 2 is not significant (P is more than 0.05), and the difference between the test group 2 and the test group 3 is not significant (P is more than 0.05); the age of 63 days, the temperature of the test group 1, the test group 2, the test group 3 and the control group are respectively 40.11 ℃, 39.13 ℃, 38.82 ℃ and 42.24 ℃, the test group 2 and the test group 3 are obviously lower than the test group 1 and the control group (P is less than 0.05), and the test group 1 is obviously lower than the control group (P is less than 0.05); the age of 63 days is 40.17 ℃, 39.54 ℃, 39.02 ℃ and 42.31 ℃ in the test groups 1, 2, 3 and the control group respectively, the test group 3 is obviously lower than the test group 1 and the control group (P is less than 0.05), the control group is obviously higher than the test groups 1, 2 and 3, the difference between the test groups 1 and 2 is not significant (P is more than 0.05), and the difference between the test group 2 and the test group 3 is not significant (P is more than 0.05).

2. The effect of heat stress on the productivity of young rabbits is shown in Table 4.

TABLE 4 influence of Heat stress Environment on the Productivity of young rabbits

Note: the data in the same row are marked with different lower case letters to indicate that the difference is significant (P <0.05), and the same or no letters to indicate that the difference is not significant (P > 0.05).

In terms of body weight: the initial weights of the test group 1, the test group 2, the test group 3 and the control group are 781.45g, 781.81g, 781.55g and 781.26g respectively at the age of 35 days, and the difference among the groups is not significant (P is more than 0.05); at the age of 49 days, the weights of the test group 1, the test group 2, the test group 3 and the control group are 961.8g, 1003.75g, 1039.20g and 886.98g respectively, the weight of the control group is obviously lower than that of the test group 1, the test group 2 and the control group 3 (P <0.05), and the weight of the test group 1 is obviously lower than that of the test group 2 and the control group 3 (P < 0.05); the weights of test groups 1, 2, 3 and control groups are 1421.58g, 1474.09g, 1519.35g and 1253.88g respectively at the age of 63 days, the control group is obviously lower than the test groups 1, 2 and 3 (P <0.05), and the test group 3 is obviously higher than the test group 1 (P < 0.05); at the age of 77 days, the weights of the test group 1, the test group 2, the test group 3 and the control group are 1782.08g, 1853.01g, 1918.81g and 1607.48g respectively, the weight of the control group is obviously lower than that of the test group 1, the test group 2 and the control group 3 (P <0.05), the weight of the test group 3 is obviously higher than that of the test group 1 and the test group 2 (P <0.05), and the weight of the test group 2 is obviously higher than that of the test group 1 (P < 0.05).

Average feed intake: in the age range of 35-49 days, the groups 1, 2 and 3 are tested, the average feed intake of a control group is 58.75g, 71.43g, 78.76g and 40.89g, the control group is obviously lower than the groups 1, 2 and 3 (P <0.05), and the group 1 is obviously lower than the groups 2 and 3 (P < 0.05); at 35-63 days of age, the control group was significantly lower than test 1, 2, 3 (P <0.05), test 3 was significantly higher than test 1 (P <0.05), and the differences between test 2 and 3 were not significant (P > 0.05); at 35-77 days of age, the control group was significantly lower than the trial 1, 2, 3 groups (P <0.05), the trial 3 group was significantly higher than the trial 1 group (P <0.05), and the differences between the trial 2 and 3 groups were not significant (P > 0.05).

Average daily gain: at 35-49 days of age, the control group was significantly lower than test 1, 2, 3 (P <0.05), test 3 was significantly higher than test 1 (P <0.05), and the differences between test 2 and 3 were not significant (P > 0.05); at 35-63 days of age, the control group was significantly lower than test 1, 2, 3 (P <0.05), test 3 was significantly higher than test 1 (P <0.05), and the differences between test 2 and 3 were not significant (P > 0.05); at 35-77 days of age, the control group was significantly lower than the trial 1, 2, 3 groups (P <0.05), the trial 3 group was significantly higher than the trial 1 group (P <0.05), and the differences between the trial 2 and 3 groups were not significant (P > 0.05).

Feed conversion rate: at 35-49 days of age, the test 3 group was significantly lower than the test 1, 2 and control (P <0.05), the control was significantly higher than the test 1, 2, 3 (P <0.05), and the difference between the test 1 and 2 groups was not significant (P > 0.05); at 35-63 days of age, the control group was significantly higher than the trial 1, 2, 3 groups (P <0.05), with the trial 3 group being the lowest but not significantly different from the trial 1, 2 groups (P > 0.05); at 35-77 days of age, the trial 2, 3 groups were significantly lower than the trial 1 group and the control group (P <0.05), with no significant difference between the trial 2, 3 groups (P > 0.05).

Survival rate: in the age range of 35-49 days, the test groups 1, 2 and 3 and the control group are respectively 90.00%, 94.00% and 84.00%, the control group is obviously lower than the test groups 1, 2 and 3 (P <0.05), and the test groups 2 and 3 are obviously higher than the test group 1 (P < 0.05); in the age range of 35-63 days, 84.00%, 88.00%, 90.00% and 76.00% are respectively used for test 1, 2 and 3 groups and a control group, the control group is obviously lower than the test 1, 2 and 3 groups (P <0.05), the test 2 and 3 groups are obviously higher than the test 1 group (P <0.05), and the difference between the test 2 and 3 groups is not significant (P > 0.05); in the age range of 35-77 days, the test groups 1, 2 and 3 and the control group are 82.00%, 88.00%, 90.00% and 72.00% respectively, the control group is obviously lower than the test groups 1, 2 and 3 (P <0.05), the test groups 2 and 3 are obviously higher than the test group 1 (P <0.05), and the difference between the test groups 2 and 3 is not significant (P > 0.05).

3. The effect of heat stress environment on the superoxide dismutase (SOD) of young rabbits is shown in table 5.

TABLE 5 Effect of Heat stress Environment on the superoxide dismutase (SOD) of young rabbits

Note: the data in the same column are marked with different lower case letters to show that the difference is significant (P <0.05), and the same or no letters to show that the difference is not significant (P > 0.05); the data from the same row indicated significant differences (P <0.05) by number, and no or the same number indicated insignificant differences (P > 0.05).

The SOD values of 35 days old, test 1, 2 and 3 groups and a control group are respectively 93.32U/ml, 91.98U/ml, 93.31U/ml and 92.67U/ml, and the difference among the groups is not significant (P is more than 0.05); the SOD values of the 49 days old test groups 1, 2 and 3 and the control group are 92.61U/ml, 93.42U/ml, 97.42U/ml and 83.44U/ml respectively, the control group is obviously lower than the test groups 1, 2 and 3 (P <0.05), and the difference among the test groups 1, 2 and 3 is not significant (P > 0.05); the SOD values of the test groups 1, 2 and 3 and the control group are 91.17U/ml, 95.43U/ml, 95.21U/ml and 79.49U/ml respectively at the age of 63 days, the control group is obviously lower than the test groups 1, 2 and 3 (P <0.05), and the difference among the test groups 1, 2 and 3 is not significant (P > 0.05); at age of 77 days, test groups 1, 2 and 3 and a control group respectively have SOD values of 91.76U/ml, 94.11U/ml, 94.51U/ml and 80.31U/ml, the control group is obviously lower than the test groups 1, 2 and 3 (P <0.05), and the difference among the test groups 1, 2 and 3 is not significant (P > 0.05). The SOD values of the test groups 1, 2 and 3 are not obviously different in different age periods of days of 35, 49, 63 and 77 (P is more than 0.05); in the control group, the SOD value at 35 days is obviously higher than 49 days, 63 days and 77 days (P < 0.05).

4. The effect of heat stress environment on Catalase (CAT) in young rabbits is shown in Table 6.

TABLE 6 Effect of Heat stress Environment on Catalase (CAT) in Young rabbits

At 5 days of age, the CAT values of the test groups 1, 2 and 3 and the control group are respectively 3.37U/ml, 3.42U/ml, 3.27U/ml and 3.32U/ml, and the difference among the groups is not significant (P is more than 0.05); at the age of 49 days, the CAT values of the test groups 1, 2 and 3 and the control group are respectively 3.32U/ml, 3.35U/ml, 3.38U/ml and 3.19U/ml, and the difference among the groups is not obvious (P is more than 0.05); the CAT values of 63 days old, test 1, 2, 3 and control groups are respectively 3.36U/ml, 3.38U/ml, 3.31U/ml and 3.02U/ml, the control group is obviously lower than the test 1, 2 and 3 (P <0.05), and the difference among the test 1, 2 and 3 is not significant (P > 0.05); at age of 77 days, test groups 1, 2, 3 and control groups have CAT values of 3.26U/ml, 3.31U/ml, 3.35U/ml and 3.05U/ml respectively, the control group is significantly lower than the test groups 1, 2 and 3 (P <0.05), and the difference among the test groups 1, 2 and 3 is not significant (P > 0.05). The CAT values of the test groups 1, 2 and 3 were not significantly different in the age ranges of 35, 49, 63 and 77 (P > 0.05); in the control group, CAT value at 35 and 49 days is obviously higher than that at 63 and 77 days (P < 0.05).

5. The effect of heat stress environment on glutathione peroxidase (GSH) in young rabbits is shown in table 7.

TABLE 7 Effect of Heat stress Environment on glutathione peroxidase (GSH) in Young rabbits

The GSH values of the 35 days old, the test groups 1, 2 and 3 and the control group are 214.32U/ml, 213.76U/ml, 215.31U/ml and 214.06U/ml respectively, and the difference among the groups is not significant (P is more than 0.05); at the age of 49 days, the GSH values of the test groups 1, 2 and 3 and the control group are 212.64U/ml, 213.52U/ml, 215.78U/ml and 206.71U/ml respectively, the GSH value of the control group is obviously lower than that of the test groups 1, 2 and 3 (P <0.05), and the difference among the test groups 1, 2 and 3 is not significant (P > 0.05); the GSH values of the 63 days old, the test groups 1, 2 and 3 and the control group are 214.87U/ml, 214.46U/ml, 216.09U/ml and 204.13U/ml respectively, the GSH value of the control group is obviously lower than that of the test groups 1, 2 and 3 (P <0.05), and the difference among the test groups 1, 2 and 3 is not significant (P > 0.05); at age of 77 days, the GSH values of the test groups 1, 2 and 3 and the control group are 213.64U/ml, 214.67U/ml, 215.43U/ml and 205.43U/ml respectively, the GSH value of the control group is obviously lower than that of the test groups 1, 2 and 3 (P <0.05), and the difference among the test groups 1, 2 and 3 is not significant (P > 0.05). The GSH values of the test groups 1, 2 and 3 are not significantly different in different age periods of days 35, 49, 63 and 77 (P is more than 0.05); in the control group, GSH values at 35 days of age were significantly higher than 49, 63, 77 days of age (P < 0.05).

6. The effect of heat stress on triiodothyronine (T3) is shown in Table 8.

TABLE 8 Effect of Heat stress Environment on Triiodothyronine (T3) in Young rabbits

The age of 35 days, the test groups 1, 2 and 3 and the control group have T3 values of 2.47ng/ml, 2.52ng/ml, 2.49ng/ml and 2.51ng/ml respectively, and the difference between the groups is not significant (P > 0.05); 49 days old, test 1, 2, 3 and control groups, T3 values are respectively 2.54ng/ml, 2.48ng/ml, 2.61ng/ml and 2.14ng/ml, the control group is significantly lower than the test 1, 2 and 3 groups (P <0.05), and the difference among the test 1, 2 and 3 groups is not significant (P > 0.05); the test groups 1, 2 and 3 and the control group have the T3 values of 2.43ng/ml, 2.57ng/ml, 2.54ng/ml and 2.03ng/ml respectively at the age of 63 days, the control group is obviously lower than the test groups 1, 2 and 3 (P <0.05), and the difference among the test groups 1, 2 and 3 is not significant (P > 0.05); 77 days old, test 1, 2, 3, control, T3 values were 2.49ng/ml, 2.50ng/ml, 2.55ng/ml, 2.05ng/ml, respectively, control was significantly lower than test 1, 2, 3 (P <0.05), with no significant difference between test 1, 2, 3 (P > 0.05). The T3 values of the test groups 1, 2 and 3 were not significantly different in the different age periods of days 35, 49, 63 and 77 (P > 0.05); in the control group, the value of T3 at 35 days is significantly higher than that at 49, 63 and 77 days (P < 0.05).

7. The effect of heat stress on thyroxine (T4) is shown in table 9.

TABLE 9 Effect of Heat stress Environment on Young Rabbit thyroxine (T4)

The T4 values of 35 days old, test 1, 2, 3 and control groups are respectively 74.32ng/ml, 76.13ng/ml, 75.47ng/ml and 74.98ng/ml, and the difference among the groups is not significant (P > 0.05); 49 days old, test 1, 2, 3 and control groups, T4 values are 74.19ng/ml, 77.22ng/ml, 76.43ng/ml and 67.45ng/ml respectively, the control group is obviously lower than the test 1, 2 and 3 groups (P <0.05), and the difference among the test 1, 2 and 3 groups is not significant (P > 0.05); the test groups 1, 2 and 3 and the control group have 63 days old, the T4 values are respectively 75.01ng/ml, 76.76ng/ml, 76.98ng/ml and 63.71ng/ml, the control group is obviously lower than the test groups 1, 2 and 3 (P <0.05), and the difference among the test groups 1, 2 and 3 is not significant (P > 0.05); 77 days old, trial 1, 2, 3, control, T4 values were 74.55ng/ml, 75.49ng/ml, 75.16ng/ml, 65.23ng/ml, respectively, control was significantly lower than trial 1, 2, 3 (P <0.05), with no significant differences between trial 1, 2, 3 (P > 0.05). The T4 values of the test groups 1, 2 and 3 were not significantly different in the different age periods of days 35, 49, 63 and 77 (P > 0.05); in the control group, the value of T4 at 35 days is significantly higher than that at 49, 63 and 77 days (P < 0.05).

The analysis of the results shows that under the condition of heat stress, the body weight, the food consumption and the daily weight gain of the control group at different days of age are obviously reduced, the feed conversion rate is increased, under the condition of adding the heat stress resistant traditional Chinese medicine formula, the body weight, the food consumption and the daily weight gain of the young rabbits at different days of age are obviously improved and increased, the feed conversion rate is obviously reduced, which shows that the traditional Chinese medicine formula has positive effect on improving and increasing the production performance under the condition of heat stress, the T3 and the T4 content in the serum of the control group 49, 63 and 77 days of age are obviously reduced in the test, while the T3 and the T4 content in the serum of the test 1, 2 and 3 added with the traditional Chinese medicine formula are not obviously changed, which shows that the traditional Chinese medicine formula has the effect of stabilizing the T3 and T4 contents in the serum of the young rabbits under the condition of heat stress, the main physiological effect of the T3 and the T4 is to promote the oxidative decomposition of sugar and fat, the growth and development are promoted, so that the composition for resisting the heat stress of the young rabbits has the effect of promoting the production performance of the young rabbits.

Under the heat stress environment, due to the fact that the added Chinese medicinal ingredients such as the radix bupleuri, the radix puerariae and the wild buckwheat rhizome in the Chinese medicinal formula have an antibacterial effect, after young rabbits eat the compound feed, the compound feed has positive effects of killing pathogenic microorganisms in intestinal tracts and maintaining the intestinal health of the young rabbits, meanwhile, the effective ingredients of the Chinese medicinal materials also have an immunity enhancing effect, and the positive effects are achieved for improving the survival rate of the young rabbits under the heat stress environment. Superoxide dismutase (SOD), Catalase (CAT) and glutathione peroxidase (GSH) are key antioxidase, the activities of the superoxide dismutase (SOD), the Catalase (CAT) and the glutathione peroxidase (GSH) of 49, 63 and 77 days of age in the groups 1, 2 and 3 are kept unchanged, and the activity of a control group is obviously reduced, which shows that the added traditional Chinese medicine formula has the effects of improving the oxidation resistance of young rabbits under the condition of heat stress and reducing the damage of the heat stress to body cells. Particularly, superoxide dismutase (SOD) is used as an important animal heat resistance evaluation index, and the results of the research prove that the composition for resisting heat stress of young rabbits has the effect of resisting heat stress and improves the heat resistance of the young rabbits.

Under the condition of heat stress, the production performance index and the survival rate of young rabbits and the biochemical indexes of superoxide dismutase (SOD), Catalase (CAT), glutathione peroxidase (GSH), triiodothyronine (T3) and thyroxine (T4) are integrated, and 1000g of radix bupleuri, kudzu root, wild buckwheat rhizome and borneol are added into 1000kg of daily ration, so that the rabbit anti-heat stress additive has good anti-heat stress effect.

While the present invention has been described in detail with reference to the specific embodiments thereof, it should not be construed as limited by the scope of the present patent. Various modifications and changes may be made by those skilled in the art without inventive step within the scope of the appended claims.

Claims

1. The composition for resisting heat stress of young rabbits is characterized by comprising the following components: bupleuri radix, radix Puerariae, rhizoma Fagopyri Dibotryis and Borneolum Syntheticum;

the mass ratio of the bupleurum, the kudzuvine root, the wild buckwheat rhizome and the borneol is 200-500: 100-400-200-500-100-400.

2. The composition for resisting heat stress of young rabbits according to claim 1, wherein the mass ratio of the bupleurum root, the kudzuvine root, the wild buckwheat rhizome and the borneol is 400: 150-.

3. The composition for resisting heat stress of young rabbits according to claim 1, wherein the mass ratio of the bupleurum, the kudzuvine root, the wild buckwheat rhizome and the borneol is 300:200:300: 200.

4. The composition for resisting heat stress of young rabbits according to claim 1, wherein the mesh number of the bupleurum, the kudzuvine root, the wild buckwheat rhizome and the borneol is 200-400 meshes.

5. Use of the composition against heat stress in young rabbits according to any one of claims 1 to 4 in the breeding of young rabbits.

6. Use of the composition for resisting heat stress of young rabbits according to any one of claims 1 to 4 in heat stress resistant feeds for young rabbits.

7. A young rabbit feed comprising the composition for combating heat stress in a young rabbit according to any one of claims 1 to 4.

8. A method for resisting heat stress of young rabbits, which is characterized in that the composition for resisting heat stress of young rabbits according to any one of claims 1 to 4 is added into daily ration of young rabbits, the addition amount is 600-1500g/1000kg, and the young rabbits are fed for more than 10-14 days.

9. The method for resisting heat stress in young rabbits according to claim 8, wherein the addition amount is 1000g/1000 kg.