CN114028289A - Preparation method and application of platycodon grandiflorum extract with relieving effect - Google Patents
Preparation method and application of platycodon grandiflorum extract with relieving effect Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P29/00—Non-central analgesic, antipyretic or antiinflammatory agents, e.g. antirheumatic agents; Non-steroidal antiinflammatory drugs [NSAID]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/82—Preparation or application process involves sonication or ultrasonication
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- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Medicinal Chemistry (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Dermatology (AREA)
- Chemical & Material Sciences (AREA)
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- Microbiology (AREA)
- Birds (AREA)
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Abstract
The invention discloses a preparation method and application of a platycodon grandiflorum extract with a relieving effect. The extraction method comprises the following steps: cleaning fresh radix Platycodi, sun drying, pulverizing in a pulverizer, and sieving; mixing radix Platycodi powder and extraction solvent in a beaker, and performing ultrasonic treatment in water bath for 30min to obtain radix Platycodi extract; carrying out suction filtration on the platycodon grandiflorum extract, carrying out solid-liquid separation, carrying out vacuum rotary evaporation to obtain a concentrated solution of the platycodon grandiflorum extract, drying at a low temperature to obtain platycodon grandiflorum extract powder, and proving that the platycodon grandiflorum extract powder has the effects of relieving and resisting inflammation through efficacy evaluation. The platycodon extract prepared by the invention has the characteristics of naturalness and safety, has good relieving or anti-inflammatory effect on skin, and can be widely applied to skin care products, such as: the invention discloses emulsion, cream, skin lotion and the like, and expands the application of natural plant platycodon grandiflorum as a soothing or anti-inflammatory raw material.
Description
Technical Field
The invention relates to an extraction method and application of platycodon grandiflorum effective components with a relieving effect, and belongs to the technical field of cosmetics.
Background
Modern pharmacology proves that the platycodon grandiflorum has obvious antioxidant effect and excellent blood sugar reducing function, and has the functions of reducing blood pressure, resisting tumors and choline, promoting bile acid secretion and the like. The root of platycodon grandiflorum can be used as a medicine and has the functions of relieving cough, eliminating phlegm, ventilating the lung, expelling pus and the like, and the platycodon grandiflorum serving as a traditional Chinese medicine (medicinal materials or decoction pieces) is recorded in the Chinese pharmacopoeia. At present, research and analysis show that the main effective components in platycodon grandiflorum comprise: saponins, polysaccharides, flavonoids, polyacetylenes, etc. With the rapid rise in the demand of personal care products, the consumption demand of people for cosmetics also becomes diversified and upgraded. People pay attention to the care of sensitive skin, and the research on the soothing or anti-inflammatory effect of the platycodon grandiflorum extract in the skin care product is not reported.
Disclosure of Invention
The technical problem solved by the invention is as follows: how to extract effective components in platycodon grandiflorum to apply the platycodon grandiflorum to the field of cosmetics.
In order to solve the above technical problems, the present invention provides a method for preparing a platycodon grandiflorum extract having a soothing or anti-inflammatory effect, comprising the steps of:
step 1: sequentially cleaning fresh radix Platycodi, sun drying, pulverizing, and sieving to obtain radix Platycodi powder;
step 2: adding the platycodon grandiflorum powder into a solvent absolute ethyl alcohol or an ethanol water solution, and fully mixing to obtain a mixed solution;
and step 3: filtering the mixed solution after ultrasonic treatment, and collecting filtrate;
and 4, step 4: carrying out vacuum rotary evaporation on the filtrate to obtain a concentrated solution;
and 5: drying the concentrated solution to obtain radix Platycodi extract powder.
Preferably, the mass ratio of the platycodon grandiflorum powder to the solvent in the step 2 is 1: 1-10.
Preferably, the conditions of the ultrasound in the step 3 are as follows: the temperature is 35-55 ℃, the power is 100-200W, the frequency is 30-60kHz, and the time is 25-35 min; the filtration is as follows: filtering with 0.4-0.5 μm microporous filter paper membrane.
Preferably, the drying process parameters in the step 5 are as follows: the temperature is 30-50 ℃ and the time is 12-24 h.
The invention also provides application of the platycodon extract prepared by the preparation method of the platycodon extract with the relieving or anti-inflammatory effect in cosmetics.
Preferably, use in a model for the assessment of soothing or anti-inflammatory efficacy of a cosmetic product.
Preferably, the model for evaluating the soothing or anti-inflammatory efficacy of the cosmetic takes caenorhabditis elegans as a biological carrier, wherein the effective evaluation concentration of the platycodon grandiflorum extract is 80-100 mg/mL
Preferably, it includes the use in the preparation of a cosmetic product with soothing or anti-inflammatory efficacy.
Preferably, the cosmetics with soothing or anti-inflammatory effects include skin lotion, skin milk and skin cream.
Preferably, the cosmetic with the soothing or anti-inflammatory effect contains the platycodon grandiflorum extract in an added concentration of 0.5-2 wt%.
Compared with the prior art, the invention has the following beneficial effects:
the platycodon extract disclosed by the invention has good relieving and anti-inflammatory effects through lower biological model experimental evaluation, so that the platycodon extract disclosed by the invention can be applied to a model for evaluating the relieving or anti-inflammatory effects of cosmetics, and the prepared platycodon extract can be widely applied to relieving and anti-inflammatory skin care products, such as: the invention discloses emulsion, cream, skin lotion and the like, and expands the application of natural plant platycodon grandiflorum as a soothing or anti-inflammatory raw material.
Drawings
FIG. 1 is a graph comparing the survival rate of LPS-induced nematodes with that of the reacted extract of Platycodon grandiflorum, wherein P is 0.001;
FIG. 2 is a graph comparing the survival rate of LPS-induced nematodes with the swallowing rate of LPS-induced nematodes after the action of the Platycodon grandiflorum extract, wherein P is less than 0.05, which indicates that there is a significant difference between the two groups by statistical analysis;
FIG. 3 is a graph comparing the survival rate of lipopolysaccharide-induced nematodes with the Malondialdehyde (MDA) content of lipopolysaccharide-induced nematodes after the action of the Platycodon grandiflorum extract, wherein P is <0.001, showing a significant difference between the two groups by statistical analysis;
FIG. 4 is a graph comparing the survival rate of LPS-induced nematodes with the glutathione content of LPS-induced nematodes after the action of the Platycodon grandiflorum extract, wherein P is less than 0.01, which indicates that there is a significant difference between the two groups by statistical analysis.
Detailed Description
In order to make the invention more comprehensible, preferred embodiments are described in detail below with reference to the accompanying drawings.
Example 1
Preparing a platycodon extract:
cleaning fresh radix Platycodi, sun drying, pulverizing in a pulverizer, and sieving to obtain radix Platycodi powder; then adding the platycodon grandiflorum powder into absolute ethyl alcohol to obtain a mixed solution; and ultrasonically extracting the mixed solution under the conditions of ultrasonic time of 30min, power of 200W, frequency of 60kHz and temperature of 40 ℃. Filtering after extraction, and collecting filtrate; carrying out vacuum rotary evaporation on the filtrate, wherein the vacuum degree is 0.096MPa to obtain a concentrated solution; drying the concentrated solution at 30 deg.C to obtain radix Platycodi extract powder with extraction rate of 4.31%.
Example 2
Preparing a platycodon extract:
cleaning fresh radix Platycodi, sun drying, pulverizing in a pulverizer, and sieving to obtain radix Platycodi powder; then adding the platycodon grandiflorum powder into 90% ethanol to obtain a mixed solution; and ultrasonically extracting the mixed solution under the conditions of ultrasonic time of 30min, power of 200W, frequency of 60kHz and temperature of 40 ℃. Filtering after extraction, and collecting filtrate; carrying out vacuum rotary evaporation on the filtrate, wherein the vacuum degree is 0.096MPa to obtain a concentrated solution; drying the concentrated solution at low temperature of 30 deg.C to obtain radix Platycodi extract powder with extraction rate of 4.78%.
Example 3
Preparing a platycodon extract:
cleaning fresh radix Platycodi, sun drying, pulverizing in a pulverizer, and sieving to obtain radix Platycodi powder; then adding the platycodon grandiflorum powder into 70% ethanol to obtain a mixed solution; and ultrasonically extracting the mixed solution under the conditions of ultrasonic time of 30min, power of 200W, frequency of 60kHz and temperature of 40 ℃. Filtering after extraction, and collecting filtrate; carrying out vacuum rotary evaporation on the filtrate, wherein the vacuum degree is 0.096MPa to obtain a concentrated solution; drying the concentrated solution at low temperature of 30 deg.C to obtain radix Platycodi extract powder with extraction rate of 4.18%.
Example 4
The platycodon grandiflorum extract prepared in examples 1-3 is evaluated for soothing or anti-inflammatory effects, and the platycodon grandiflorum extract prepared in example 2 is used as a sample in the test, and the specific method and the result are as follows:
1. effect of LPS on survival Rate of caenorhabditis elegans in Presence of Platycodon grandiflorum extract
C. elegans of stage L4 were collected by exposing the nematodes to 100mg/mL of the M9 buffer solution of Platycodon grandiflorum extract for 30 minutes, then exposing the nematodes to LPS (100. mu.g/mL) for 24 hours, comparing the samples without the extract, after 24 hours, washing the individuals with M9 buffer, repeating this procedure twice, then transferring to NGM agar plates, then allowing them to acclimate for 30 minutes, and then performing survival analysis. All data were replicated 3 times, and the data were analyzed and graphed using GraphPad Prism 7. The results are shown in FIG. 1.
2. Effect of lipopolysaccharide on Switling Rate of caenorhabditis elegans in the Presence of Platycodon grandiflorum extract
C. elegans of the L4 stage of the synchronization were collected by exposing the nematodes to 100mg/mL of the M9 buffer solution of Platycodon grandiflorum extract for 30 minutes, then exposing the nematodes to LPS (100. mu.g/mL) for 24 hours, comparing samples without the extract, after 24 hours, washing the individuals with M9 buffer, repeating this procedure twice, then transferring to NGM agar plates, then allowing them to acclimate for 30 minutes, and then performing the swallowing rate analysis under the microscope. All data were replicated 3 times, and the data were analyzed and graphed using GraphPad Prism 7. The results are shown in FIG. 2.
3. Effect of lipopolysaccharide on the content of C.elegans malondialdehyde in the presence of Platycodon grandiflorum extract
C. elegans of stage L4 of the synchronization was collected by exposing the nematodes to 100mg/mL of the M9 buffer solution of Platycodon grandiflorum extract for 30 minutes, then exposing the nematodes to LPS (100. mu.g/mL) for 24 hours, comparing the samples without the extract, after 24 hours, washing the individuals with M9 buffer, repeating this step twice, collecting the nematodes and sonicating in ice-cold lysis buffer, and centrifuging to obtain the supernatant. The malonaldehyde measurement was performed using a malonaldehyde kit, according to the kit method. All data were replicated 3 times, and the data were analyzed and graphed using GraphPad Prism 7. The results are shown in FIG. 3.
4. Effect of lipopolysaccharide on glutathione content of caenorhabditis elegans in the presence of Platycodon grandiflorum extract
C. elegans of stage L4 of the synchronization was collected by exposing the nematodes to 100mg/mL of the M9 buffer solution of Platycodon grandiflorum extract for 30 minutes, then exposing the nematodes to LPS (100. mu.g/mL) for 24 hours, comparing the samples without the extract, after 24 hours, washing the individuals with M9 buffer, repeating this step twice, collecting the nematodes and sonicating in ice-cold lysis buffer, and centrifuging to obtain the supernatant. The content of the glutathione is measured by adopting a glutathione kit according to a kit method. All data were replicated 3 times, and the data were analyzed and graphed using GraphPad Prism 7. The results are shown in FIG. 4.
5. Biochemical indicator analysis of nematode
As shown in fig. 1, data statistics show that the 24-hour survival rate of nematodes in the LPS induction + platycodon grandiflorum extract treatment group is improved by 24% compared with that in a single LPS induction group; as shown in fig. 2, data statistics found that the 24-hour swallowing rate of the LPS-treated + platycodon extract-treated group was increased by 35% relative to that of the LPS-treated group alone; as shown in fig. 3, data statistics show that compared with a single LPS-induced group, the MDA content of nematodes in the lipopolysaccharide-induced + platycodon grandiflorum extract-treated group was reduced by 37%; as shown in fig. 4, data statistics show that the GSH content of the nematodes in the LPS-induced + platycodon grandiflorum extract-treated group was increased by 34.8% compared to the LPS-induced group alone. Statistical data show that the survival rate, the swallowing rate and the glutathione content of the nematodes treated by the platycodon grandiflorum extract are remarkably increased, the malondialdehyde content is remarkably reduced, the platycodon grandiflorum extract remarkably slows down the inflammation induction effect of lipopolysaccharide on the nematodes, and the platycodon grandiflorum extract has the effects of relieving and resisting inflammation.
Application example 1
A skin care emulsion containing platycodon grandiflorum extract comprises the following raw materials in percentage by mass:
phase A
Caprylic capric acid triglyceride 2
Stearyl alcohol polyoxyethylene ether-721
Stearyl alcohol polyoxyethylene ether-7219
BHT 0.2
Phase B
Hyaluronic acid sodium salt 0.1
Xanthan gum 0.2
Adding deionized water to 100 wt%
Phase C
0.5 parts of phenoxyethanol
Hexanediol 0.5
The preparation method of the skin care emulsion comprises the following steps:
the method comprises the following steps: respectively weighing the phase A and the phase B, heating to 80 ℃, and stirring for dissolving;
step two: slowly add phase a to phase B, homogenize in a homogenizer at 13000rpm for 10min to complete emulsification, then continue mechanical stirring.
Step four: adding phase C when the temperature is reduced to 50 deg.C, stirring for 10min, and cooling to room temperature to obtain skin care emulsion.
Application example 2
A skin care cream containing platycodon grandiflorum extract comprises the following raw materials in percentage by mass:
phase A
Caprylic capric acid triglyceride 2
Stearyl alcohol polyoxyethylene ether-721
Stearyl alcohol polyoxyethylene ether-7212
BHT 0.2
Phase B
Hyaluronic acid sodium salt 0.1
Glycerol 7
Xanthan gum 0.5
Adding deionized water to 100 wt%
Phase C
0.5 parts of phenoxyethanol
Hexanediol 0.5
The preparation method of the skin-care cream comprises the following steps:
the method comprises the following steps: respectively weighing the phase A and the phase B, heating to 80 ℃, and stirring for dissolving;
step two: slowly add phase a to phase B, homogenize in a homogenizer at 13000rpm for 10min to complete emulsification, then continue mechanical stirring.
Step four: adding phase C when the temperature is reduced to 50 deg.C, stirring for 10min, and cooling to room temperature to obtain skin care emulsion.
Application example 3
A skin care lotion containing platycodon grandiflorum extract comprises the following raw materials in percentage by mass:
phase A
1, 3-butanediol 6
Dipropylene glycol 4
Phase B
Laureth-151
Adding deionized water to 100%
Phase C
Essence 0.25
Hexanediol 0.5
0.5 parts of phenoxyethanol
The preparation method of the skin lotion comprises the following steps:
the method comprises the following steps: respectively weighing the phase A and the phase B, heating to 65 ℃, and uniformly stirring;
step two: add phase A to phase B, homogenize for 10min in a homogenizer at 13000rpm, then continue mechanical stirring.
Step four: adding the C phase when the temperature is reduced to 50 ℃, continuously stirring for 10min, and cooling to room temperature to obtain the skin lotion.
While the invention has been described with respect to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention.
Claims (10)
1. A preparation method of a platycodon grandiflorum extract with a soothing or anti-inflammatory effect is characterized by comprising the following steps:
step 1: sequentially cleaning fresh radix Platycodi, sun drying, pulverizing, and sieving to obtain radix Platycodi powder;
step 2: adding the platycodon grandiflorum powder into a solvent absolute ethyl alcohol or an ethanol water solution, and fully mixing to obtain a mixed solution;
and step 3: filtering the mixed solution after ultrasonic treatment, and collecting filtrate;
and 4, step 4: carrying out vacuum rotary evaporation on the filtrate to obtain a concentrated solution;
and 5: drying the concentrated solution to obtain radix Platycodi extract powder.
2. The method for preparing a platycodon grandiflorum extract having a soothing or anti-inflammatory effect according to claim 1, wherein the mixing ratio of the platycodon grandiflorum powder to the solvent in the step 2 is 1: 1-10.
3. The method for preparing platycodon grandiflorum extract having a soothing or anti-inflammatory effect according to claim 1, wherein the ultrasonic conditions in the step 3 are as follows: the temperature is 35-55 ℃, the power is 100-200W, the frequency is 30-60kHz, and the time is 25-35 min; the filtration is as follows: filtering with 0.4-0.5 μm microporous filter paper membrane.
4. The method for preparing platycodon grandiflorum extract having a soothing or anti-inflammatory effect according to claim 1, wherein the drying process parameters in the step 5 are as follows: the temperature is 30-50 ℃ and the time is 12-24 h.
5. Use of the platycodon grandiflorum extract prepared by the method for preparing a platycodon grandiflorum extract having a soothing or anti-inflammatory effect according to any one of claims 1 to 4 in cosmetics.
6. Use according to claim 5, comprising use in a model for the assessment of soothing or anti-inflammatory efficacy of a cosmetic product.
7. The use of claim 6, wherein the model for evaluating soothing or anti-inflammatory efficacy of a cosmetic is caenorhabditis elegans as a biological carrier, wherein the effective evaluation concentration of the Platycodon grandiflorum extract is 80-100 mg/mL.
8. Use according to claim 5, comprising the use in the preparation of a cosmetic product with soothing or anti-inflammatory effects.
9. Use according to claim 8, wherein the soothing or anti-inflammatory cosmetic agents include skin lotions, creams and lotions.
10. The use according to claim 9, wherein the platycodon grandiflorum extract is added in a concentration of 0.5 to 2 wt% in the cosmetic with soothing or anti-inflammatory effects.
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Cited By (1)
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| CN116637055A (en) * | 2023-07-13 | 2023-08-25 | 广州芭妍化妆品有限公司 | Plant itching relieving liquid for infants and preparation method thereof |
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Application publication date: 20220211 |