CN114027328A - Pesticide granules for preventing and treating clubroot and root-knot nematode of cruciferous vegetables - Google Patents

Pesticide granules for preventing and treating clubroot and root-knot nematode of cruciferous vegetables Download PDF

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CN114027328A
CN114027328A CN202111341631.6A CN202111341631A CN114027328A CN 114027328 A CN114027328 A CN 114027328A CN 202111341631 A CN202111341631 A CN 202111341631A CN 114027328 A CN114027328 A CN 114027328A
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parts
clubroot
garlic
extracting
preventing
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陈小均
陈文�
何海永
吴石平
詹金碧
廖国会
何永福
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GUIZHOU INSTITUTE OF PLANT PROTECTION
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GUIZHOU INSTITUTE OF PLANT PROTECTION
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Abstract

The invention belongs to the technical field of pesticides, and discloses a pesticide granule for preventing and treating clubroot and meloidogyne of cruciferous vegetables, which comprises, by mass, 30-40 parts of fluazinam, 20-30 parts of artemisia capillaris, 10-15 parts of garlic oil, 10-15 parts of Chinese torreya seeds, 10-15 parts of pomegranate bark extract, 6-12 parts of indolebutyric acid, 5-10 parts of clothianidin, 1-2 parts of chitinase inhibitor and 1-2 parts of biological control microbial inoculum. The pesticide granules for preventing and treating the clubroot and the root-knot nematodes of the cruciferous vegetables can effectively prevent and treat the clubroot, the clubroot and underground pests, avoid pollution, improve the quality of the vegetables, delay the generation of resistance of the root-knot nematodes to chemical reagents, have obvious prevention and treatment effects, and reduce the usage amount of pesticides, thereby reducing the cost and reducing the pollution to the environment. The preparation method is simple, convenient to use, low in cost and free of pollution.

Description

Pesticide granules for preventing and treating clubroot and root-knot nematode of cruciferous vegetables
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a pesticide granule for preventing and treating clubroot and root-knot nematode of cruciferous vegetables.
Background
At present, more than 80 kinds of root-knot nematodes are reported internationally, mainly parasitize over 20000 kinds of hosts such as vegetables, fruit trees, economic crops, grain crops, ornamental plants, weeds and the like, plasmodiophora belongs to weak parasitic fungi, namely, the plasmodiophora can survive and infect in many adverse environments, has particularly strong vitality and can survive in soil for more than 10 years. Clubroot and root knot nematode disease are two major root diseases which are prominent in crop production, particularly vegetable production, and tumors with different sizes are formed at roots to cause root malformation, so that overground parts are finally slow in growth, short, water-deficient, fertilizer-deficient and small and old seedling states. With the progress of the disease, the leaves gradually become withered and yellow, and the death of the whole plant can be caused seriously. Because the two diseases have cross-onset and have a plurality of common parts, the two diseases are often confused by growers in production, and the two diseases cannot be effectively prevented and treated.
The host range is wide, clubroot mostly exists in cruciferae, the life cycle of the first stage can be completed in host plants of non-cruciferae, the quality and yield of vegetables are seriously damaged, the yield is reduced by 25% if the weight is reduced, and the weight is more than 85% if the weight is reduced. The host range of the root knot nematode disease is wider, more than 2000 roots are obtained, and the root knot nematode disease mainly comprises root knot nematode of melon and fruit (javnica) (harmful to tomatoes), root knot nematode of south China (incognita), root knot nematode of north China (hapla), root knot nematode of peanut (arenaria) and the like.
Although the existing patent uses pesticides for preventing and treating clubroot and meloidogyne as main materials, the existing patent uses chemical pesticides as main materials, so that the existing patent can easily cause drug resistance of pathogenic bacteria, and is also large in pollution and harmful to human health; the existing biological control method can only control single clubroot or root knot nematode disease generally.
Through the above analysis, the problems and defects of the prior art are as follows: in the prior art, chemical pesticides are mainly used, so that not only can pathogenic bacteria easily generate drug resistance, but also pollution is high, and human health is harmed; the existing biological control method can only control single clubroot or root knot nematode disease generally.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a pesticide granule for preventing and treating clubroot and root-knot nematode of cruciferous vegetables.
The invention is realized in such a way that a preparation method of pesticide granules for preventing and treating clubroot and root-knot nematodes of cruciferous vegetables comprises the following steps:
step one, preparing a biological control microbial inoculum consisting of bacillus methylotrophicus, purple lilac spore bacteria, trichoderma harzianum and marigold straws: mixing the bacillus methylotrophicus seed liquid and the purple violet spore bacterium seed liquid according to a proportion, inoculating the mixture into a fermentation culture medium according to the inoculation amount of 6% of the volume ratio, and culturing for 36-48h at room temperature to obtain bacillus methylotrophicus-purple spore bacterium mixed fermentation liquid; inoculating the Trichoderma harzianum seed liquid into a fermentation culture medium according to the inoculation amount of 10% by volume, and culturing at 28 ℃ for 36-48h to obtain Trichoderma harzianum fermentation liquid; uniformly mixing a methylotrophic bacillus-lilac spore fungus mixed fermentation broth and a trichoderma harzianum fermentation broth according to a ratio to obtain a mixed microbial inoculum; smashing marigold straws, sieving with a 40-50 mesh sieve, and uniformly mixing the smashed marigold straws serving as a carrier with the mixed microbial inoculum to obtain a biological control microbial inoculum for later use;
removing impurities from stems, leaves and seeds of artemisia capillaries harvested in winter, drying in the sun, and preparing an artemisia capillaries extract by crushing, extracting, concentrating and drying: removing impurities from stems, leaves and seeds of herba Artemisiae Scopariae harvested in winter, sun drying, and pulverizing the sun dried herba Artemisiae Scopariae with a pulverizer; adding mixed solvent into pulverized herba Artemisiae Scopariae, extracting for 24-36 hr with shaking table, and centrifuging with centrifuge to obtain supernatant and residue; adding methanol into the residue, soaking, and extracting at room temperature for 24-36 h; centrifuging by a centrifuge to obtain supernatant and residue; mixing the supernatants, adding appropriate amount of plant ash, stirring, filtering, concentrating under reduced pressure, and vacuum drying to obtain herba Artemisiae Scopariae extract;
step three, preparing a pomegranate bark extracting solution by using pomegranate bark: washing fresh or dried natural pericarpium Granati with flowing water for 2-3 times, and drying; crushing the dried pomegranate rind by using a crusher; extracting the crushed pomegranate rind by using acetic acid to obtain a pomegranate rind primary extracting solution, roughly filtering the pomegranate rind primary extracting solution by using a 200-mesh and 220-mesh metal screen, and removing filter residues; carrying out vacuum filtration on the filtered primary pomegranate bark extracting solution, and collecting supernatant to obtain a pomegranate bark extracting solution for later use;
step four, cleaning and pulping garlic, performing enzymolysis, extracting, distilling under reduced pressure and purifying to obtain garlic oil; weighing herba Artemisiae Scopariae, semen Torreyae, pericarpium Granati extract, indolebutyric acid, clothianidin, chitinase inhibitor and biological control microbial inoculum according to a certain proportion;
step five, uniformly mixing the weighed fluazinam, artemisia capillaries, Chinese torreya seeds, pomegranate bark extract, indolebutyric acid, clothianidin, chitinase inhibitor and biological control microbial inoculum, carrying out superfine grinding, and dissolving in garlic oil to obtain a pesticide mixture; and (4) granulating the pesticide mixture to obtain pesticide granules.
Further, in the first step, the bacillus methylotrophicus seed liquid and the lilac spore fungus seed liquid are mixed according to the proportion of 2:3, and the bacillus methylotrophicus-lilac spore fungus mixed fermentation liquid and the trichoderma harzianum fermentation liquid are mixed according to the volume ratio of 4: 5.
Further, in the second step, the mixed solvent is obtained by mixing ethanol and dichloromethane according to the ratio of 3: 1.
Further, in the second step, the vacuum drying temperature is 90-95 ℃, and the vacuum drying time is 20-30 min.
Further, in the third step, the conditions for extracting pomegranate rind with acetic acid include: extracting at 55 deg.C for 10-12 h.
Further, in the fourth step, the garlic oil is obtained by cleaning, pulping, enzymolysis, extraction, reduced pressure distillation and purification of garlic, and comprises the following steps:
selecting healthy garlic, splitting and peeling the garlic, cleaning the garlic with clear water, draining the water, and beating the garlic into garlic pulp at the temperature of 10-30 ℃;
adding active enzyme and cellulase into the obtained garlic pulp for enzymolysis to obtain enzymolysis liquid and filter residue; adding the enzymatic hydrolysate into a reaction tank, adding soybean oil, fully and uniformly mixing, and leaching;
cooling, filtering to remove residue, standing, filtering, and extracting upper layer of garlic oil supernatant; and (4) carrying out centrifugal separation on the garlic oil supernatant to obtain the garlic oil.
Further, the enzymolysis conditions are as follows: performing enzymolysis at 40-45 deg.C for 20-30 min; the leaching conditions are as follows: leaching at 80-105 deg.C for 90-120 min.
The invention also aims to provide the brassicaceous vegetable clubroot and root knot nematode prevention and control pesticide granule prepared by the preparation method of the brassicaceous vegetable clubroot and root knot nematode prevention and control pesticide granule, and the brassicaceous vegetable clubroot and root knot nematode prevention and control pesticide granule is composed of 30-40 parts of fluazinam, 20-30 parts of artemisia capillaris, 10-15 parts of garlic oil, 10-15 parts of Chinese torreya seeds, 10-15 parts of pomegranate bark extract, 6-12 parts of indolebutyric acid, 5-10 parts of clothianidin, 1-2 parts of chitinase inhibitor and 1-2 parts of biological prevention and control fungicide in parts by mass.
Further, the biological control microbial inoculum consists of bacillus methylotrophicus, purple lilac spore bacteria, trichoderma harzianum and marigold straws.
The invention also aims to provide application of the pesticide granules for preventing and treating the clubroot and the root knot nematode of the cruciferous vegetables to prevention and treatment of the clubroot and the root knot nematode of the cruciferous vegetables.
By combining all the technical schemes, the invention has the advantages and positive effects that: the pesticide granules for preventing and treating the clubroot and the root-knot nematodes of the cruciferous vegetables can effectively prevent and treat the clubroot, the clubroot and underground pests, avoid pollution, improve the quality of the vegetables, delay the generation of resistance of the root-knot nematodes to chemical reagents, have obvious prevention and treatment effects, and reduce the usage amount of pesticides, thereby reducing the cost and reducing the pollution to the environment. The preparation method is simple, convenient to use, low in cost and free of pollution.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings needed to be used in the embodiments of the present application will be briefly described below, and it is obvious that the drawings described below are only some embodiments of the present application, and it is obvious for those skilled in the art that other drawings can be obtained from the drawings without creative efforts.
FIG. 1 is a flow chart of a preparation method of pesticide granules for preventing and treating clubroot and root-knot nematode of cruciferous vegetables, which is provided by the embodiment of the invention.
FIG. 2 is a flow chart of a method for preparing a mixed microbial inoculum consisting of Bacillus methylotrophicus, purple lilac spore and Trichoderma harzianum according to an embodiment of the invention.
Fig. 3 is a flow chart of a method for preparing artemisia capillaris thunb extract by removing impurities from stems, leaves and seeds of artemisia capillaris thunb harvested in winter, drying the artemisia capillaris thunb in the sun, and crushing, extracting, concentrating and drying the artemisia capillaris thunb.
Fig. 4 is a flowchart of a method for preparing an extracting solution of pomegranate bark by using pomegranate bark according to an embodiment of the present invention.
Fig. 5 is a flow chart of a method for cleaning, pulping, performing enzymolysis, extracting, distilling under reduced pressure and purifying garlic to obtain garlic oil according to an embodiment of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Aiming at the problems in the prior art, the invention provides a pesticide granule for preventing and treating clubroot and root-knot nematode of cruciferous vegetables, which is described in detail with reference to the attached drawings.
The pesticide granules for preventing and treating clubroot and root-knot nematode of cruciferous vegetables provided by the embodiment of the invention comprise, by mass, 30-40 parts of fluazinam, 20-30 parts of artemisia capillaris, 10-15 parts of garlic oil, 10-15 parts of Chinese torreya seeds, 10-15 parts of pomegranate bark extract, 6-12 parts of indolebutyric acid, 5-10 parts of clothianidin, 1-2 parts of chitinase inhibitor and 1-2 parts of biological control microbial inoculum.
The biological control microbial inoculum provided by the embodiment of the invention consists of bacillus methylotrophicus, purple lilac spore bacteria, trichoderma harzianum and marigold straws.
As shown in fig. 1, the preparation method of the pesticide granules for preventing and treating clubroot and root-knot nematode of brassicaceous vegetables provided by the embodiment of the invention comprises the following steps:
s101, preparing a mixed microbial inoculum consisting of bacillus methylotrophicus, purple lilac spore bacteria and trichoderma harzianum, crushing marigold straws, sieving with a 40-50-mesh sieve, and uniformly mixing the crushed marigold straws serving as a carrier with the mixed microbial inoculum to obtain a biological control microbial inoculum;
s102, removing impurities from stems, leaves and seeds of artemisia capillaries harvested in winter, drying in the sun, and preparing an artemisia capillaries extract through crushing, extracting, concentrating and drying; meanwhile, preparing a pomegranate bark extracting solution by using pomegranate bark;
s103, cleaning and pulping garlic, performing enzymolysis, extracting, distilling under reduced pressure and purifying to obtain garlic oil; weighing herba Artemisiae Scopariae, semen Torreyae, pericarpium Granati extract, indolebutyric acid, clothianidin, chitinase inhibitor and biological control microbial inoculum according to a certain proportion;
s104, uniformly mixing the weighed artemisia capillaris, the Chinese torreya seeds, the pomegranate bark extracting solution, indolebutyric acid, clothianidin, the chitinase inhibitor and the biological control microbial inoculum, carrying out superfine grinding, and dissolving in garlic oil to obtain a pesticide mixture; and (4) granulating the pesticide mixture to obtain pesticide granules.
As shown in fig. 2, the preparation of the mixed bacterial preparation consisting of bacillus methylotrophicus, rhodosporidium lilacinum and trichoderma harzianum according to the embodiment of the present invention includes:
s201, mixing a bacillus methylotrophicus seed solution and a violet spore bacterium seed solution according to a ratio of 2:3, inoculating the mixture into a fermentation culture medium according to an inoculation amount of 6% in volume ratio, and culturing at room temperature for 36-48h to obtain a bacillus methylotrophicus-violet spore bacterium mixed fermentation liquid;
s202, inoculating the Trichoderma harzianum seed liquid into a fermentation medium according to the inoculation amount of 10% by volume, and culturing at 28 ℃ for 36-48h to obtain a Trichoderma harzianum fermentation liquid;
s203, uniformly mixing the bacillus methylotrophicus-purple lilac spore fungus mixed fermentation liquid and the trichoderma harzianum fermentation liquid according to the volume ratio of 4:5 to obtain the mixed microbial inoculum.
As shown in fig. 3, the artemisia capillaris thunb extract provided by the embodiment of the present invention, which is prepared by removing impurities from stems, leaves and seeds of artemisia capillaris thunb harvested in winter, drying in the sun, pulverizing, extracting, concentrating and drying, comprises:
s301, removing impurities from stems, leaves and seeds of the artemisia capillaries harvested in winter, drying the artemisia capillaries in the sun, and crushing the dried artemisia capillaries by a crusher;
s302, adding ethanol and dichloromethane into the crushed artemisia capillaris thumb to be mixed according to the ratio of 3:1, shaking and leaching for 24-36 hours by using a shaking table, and centrifuging by using a centrifugal machine to obtain supernate and residues;
s303, adding methanol into the residue, soaking, and extracting at room temperature for 24-36 h; centrifuging by a centrifuge to obtain supernatant and residue;
s304, mixing the supernatants obtained in the two extractions, adding a proper amount of plant ash, uniformly stirring, filtering, concentrating under reduced pressure, and drying in vacuum to obtain the artemisia capillaris extract.
The vacuum drying temperature provided by the embodiment of the invention is 90-95 ℃, and the vacuum drying time is 20-30 min.
As shown in fig. 4, the preparation of the pomegranate bark extract solution by using pomegranate bark according to the embodiment of the present invention includes:
s401, washing fresh or dry natural pomegranate rind with flowing water for 2-3 times and then drying; crushing the dried pomegranate rind by using a crusher;
s402, extracting the crushed pomegranate rind for 10-12 hours at 55 ℃ by using acetic acid to obtain a pomegranate rind primary extracting solution, roughly filtering the pomegranate rind primary extracting solution by using a 200-mesh and 220-mesh metal screen, and removing filter residues;
and S403, carrying out vacuum filtration on the filtered primary pomegranate bark extracting solution, and collecting supernatant to obtain the pomegranate bark extracting solution.
As shown in fig. 5, the garlic oil obtained by cleaning, pulping, performing enzymolysis, extracting, distilling under reduced pressure, and purifying garlic provided by the embodiment of the present invention includes:
s501, selecting healthy garlic, splitting and peeling the garlic, cleaning the garlic with clear water, draining the water, and beating the garlic into garlic pulp at the temperature of 10-30 ℃;
s502, adding active enzyme and cellulase into the obtained garlic pulp, and performing enzymolysis at 40-45 ℃ for 20-30min to obtain enzymolysis liquid and filter residue;
s503, adding the enzymatic hydrolysate into a reaction tank, then adding soybean oil, and fully and uniformly mixing; leaching at 80-105 deg.C for 90-120 min;
s504, cooling, filtering to remove residues, standing, filtering, and extracting the upper layer of garlic oil supernatant; and (4) carrying out centrifugal separation on the garlic oil supernatant to obtain the garlic oil.
In the description of the present invention, "a plurality" means two or more unless otherwise specified; the terms "upper", "lower", "left", "right", "inner", "outer", "front", "rear", "head", "tail", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are only for convenience in describing and simplifying the description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed in a particular orientation, and be operated, and thus, should not be construed as limiting the invention. Furthermore, the terms "first," "second," "third," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention, and the scope of the present invention is not limited thereto, and any modification, equivalent replacement, and improvement made by those skilled in the art within the technical scope of the present invention disclosed herein, which is within the spirit and principle of the present invention, should be covered by the present invention.

Claims (10)

1. A preparation method of pesticide granules for preventing and treating clubroot and meloidogyne of cruciferous vegetables is characterized in that the preparation method of the pesticide granules for preventing and treating clubroot and meloidogyne of cruciferous vegetables comprises the following steps:
step one, preparing a biological control microbial inoculum consisting of bacillus methylotrophicus, purple lilac spore bacteria, trichoderma harzianum and marigold straws: mixing the bacillus methylotrophicus seed liquid and the purple violet spore bacterium seed liquid according to a proportion, inoculating the mixture into a fermentation culture medium according to the inoculation amount of 6% of the volume ratio, and culturing for 36-48h at room temperature to obtain bacillus methylotrophicus-purple spore bacterium mixed fermentation liquid; inoculating the Trichoderma harzianum seed liquid into a fermentation culture medium according to the inoculation amount of 10% by volume, and culturing at 28 ℃ for 36-48h to obtain Trichoderma harzianum fermentation liquid; uniformly mixing a methylotrophic bacillus-lilac spore fungus mixed fermentation broth and a trichoderma harzianum fermentation broth according to a ratio to obtain a mixed microbial inoculum; smashing marigold straws, sieving with a 40-50 mesh sieve, and uniformly mixing the smashed marigold straws serving as a carrier with the mixed microbial inoculum to obtain a biological control microbial inoculum for later use;
removing impurities from stems, leaves and seeds of artemisia capillaries harvested in winter, drying in the sun, and preparing an artemisia capillaries extract by crushing, extracting, concentrating and drying: removing impurities from stems, leaves and seeds of herba Artemisiae Scopariae harvested in winter, sun drying, and pulverizing the sun dried herba Artemisiae Scopariae with a pulverizer; adding mixed solvent into pulverized herba Artemisiae Scopariae, extracting for 24-36 hr with shaking table, and centrifuging with centrifuge to obtain supernatant and residue; adding methanol into the residue, soaking, and extracting at room temperature for 24-36 h; centrifuging by a centrifuge to obtain supernatant and residue; mixing the supernatants, adding appropriate amount of plant ash, stirring, filtering, concentrating under reduced pressure, and vacuum drying to obtain herba Artemisiae Scopariae extract;
step three, preparing a pomegranate bark extracting solution by using pomegranate bark: washing fresh or dried natural pericarpium Granati with flowing water for 2-3 times, and drying; crushing the dried pomegranate rind by using a crusher; extracting the crushed pomegranate rind by using acetic acid to obtain a pomegranate rind primary extracting solution, roughly filtering the pomegranate rind primary extracting solution by using a 200-mesh and 220-mesh metal screen, and removing filter residues; carrying out vacuum filtration on the filtered primary pomegranate bark extracting solution, and collecting supernatant to obtain a pomegranate bark extracting solution for later use;
step four, cleaning and pulping garlic, performing enzymolysis, extracting, distilling under reduced pressure and purifying to obtain garlic oil; weighing herba Artemisiae Scopariae, semen Torreyae, pericarpium Granati extract, indolebutyric acid, clothianidin, chitinase inhibitor and biological control microbial inoculum according to a certain proportion;
step five, uniformly mixing the weighed fluazinam, artemisia capillaries, Chinese torreya seeds, pomegranate bark extract, indolebutyric acid, clothianidin, chitinase inhibitor and biological control microbial inoculum, carrying out superfine grinding, and dissolving in garlic oil to obtain a pesticide mixture; and (4) granulating the pesticide mixture to obtain pesticide granules.
2. The method for preparing pesticide granules for preventing and treating clubroot and meloidogyne of cruciferous vegetables according to claim 1, wherein in the first step, the bacillus methylotrophicus seed solution and the violonyea lilacina seed solution are mixed according to a ratio of 2:3, and the bacillus methylotrophicus-violonyea lilacina mixed fermentation liquor and the trichoderma harzianum fermentation liquor are mixed according to a volume ratio of 4: 5.
3. The method for preparing pesticide granules for preventing and treating clubroot and meloidogyne of cruciferous vegetables according to claim 1, wherein in the second step, the mixed solvent is obtained by mixing ethanol and dichloromethane according to a ratio of 3: 1.
4. The method for preparing pesticide granules for preventing and treating clubroot and meloidogyne of cruciferous vegetables according to claim 1, wherein in the second step, the vacuum drying temperature is 90-95 ℃, and the vacuum drying time is 20-30 min.
5. The method for preparing pesticide granules for preventing and treating clubroot and meloidogyne of cruciferous vegetables according to claim 1, wherein in the third step, the conditions for extracting pomegranate rind by using acetic acid comprise: extracting at 55 deg.C for 10-12 h.
6. The method for preparing pesticide granules for preventing and treating clubroot and meloidogyne of cruciferous vegetables according to claim 1, wherein in the fourth step, the garlic oil obtained by cleaning and pulping garlic, performing enzymolysis, extracting, distilling under reduced pressure and purifying comprises the following steps:
selecting healthy garlic, splitting and peeling the garlic, cleaning the garlic with clear water, draining the water, and beating the garlic into garlic pulp at the temperature of 10-30 ℃;
adding active enzyme and cellulase into the obtained garlic pulp for enzymolysis to obtain enzymolysis liquid and filter residue; adding the enzymatic hydrolysate into a reaction tank, adding soybean oil, fully and uniformly mixing, and leaching;
cooling, filtering to remove residue, standing, filtering, and extracting upper layer of garlic oil supernatant; and (4) carrying out centrifugal separation on the garlic oil supernatant to obtain the garlic oil.
7. The preparation method of the pesticide granules for preventing and treating clubroot and meloidogyne of cruciferous vegetables according to claim 6, wherein the conditions of the enzymolysis are as follows: performing enzymolysis at 40-45 deg.C for 20-30 min; the leaching conditions are as follows: leaching at 80-105 deg.C for 90-120 min.
8. The brassicaceous vegetable clubroot and root knot nematode prevention pesticide granule prepared by the preparation method of the brassicaceous vegetable clubroot and root knot nematode prevention pesticide granule according to any one of claims 1-7, which is characterized by comprising, by mass, 30-40 parts of fluazinam, 20-30 parts of artemisia capillaries, 10-15 parts of garlic oil, 10-15 parts of Chinese torreya seeds, 10-15 parts of pomegranate bark extract, 6-12 parts of indolebutyric acid, 5-10 parts of clothianidin, 1-2 parts of chitinase inhibitor and 1-2 parts of biological prevention and control fungicide.
9. The pesticide granule for controlling clubroot and root-knot nematode of cruciferous vegetables according to claim 8, wherein the biological control microbial inoculum consists of bacillus methylotrophicus, violaxanthus lilacinus, trichoderma harzianum and marigold straw.
10. Use of the granular pesticide for controlling the clubroot and the root knot nematode of cruciferous vegetables according to any one of claims 8 to 9 for controlling the clubroot and the root knot nematode of cruciferous vegetables.
CN202111341631.6A 2021-11-12 2021-11-12 Pesticide granules for preventing and treating clubroot and root-knot nematode of cruciferous vegetables Pending CN114027328A (en)

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Application publication date: 20220211