CN106635884A - Separation for endophytic actinomycetes of kiwifruit roots and application thereof - Google Patents
Separation for endophytic actinomycetes of kiwifruit roots and application thereof Download PDFInfo
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- CN106635884A CN106635884A CN201610999919.5A CN201610999919A CN106635884A CN 106635884 A CN106635884 A CN 106635884A CN 201610999919 A CN201610999919 A CN 201610999919A CN 106635884 A CN106635884 A CN 106635884A
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- root
- fructus actinidiae
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- actinidiae chinensiss
- kiwifruit
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- 244000298697 Actinidia deliciosa Species 0.000 title abstract description 14
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- 241000186361 Actinobacteria <class> Species 0.000 title abstract 5
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- 238000000855 fermentation Methods 0.000 claims abstract description 15
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- 244000052616 bacterial pathogen Species 0.000 claims abstract description 6
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 8
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/465—Streptomyces
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/02—Separating microorganisms from their culture media
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- Life Sciences & Earth Sciences (AREA)
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- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Pest Control & Pesticides (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Plant Pathology (AREA)
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Abstract
The invention discloses endophytic actinomycetes of kiwifruit roots and an application of the endophytic actinomycetes of the kiwifruit roots. The endophytic actinomycetes of the kiwifruit roots are Streptomyces globisporus and named as Streptomyces globisporus SCAU-1, and the collection number of the Streptomyces globisporus SCAU-1 is CCTCC NO: M 2016577. According to the invention, functional endophytic actinomycetes are separated from roots of kiwifruit plants suffering from cankers, thereby promoting plant growth, further being capable of obviously inhibiting the growing abilities of plant pathogenic fungi and pathogenic bacteria, and being capable of being used for preparing a fertilizer-pesticide combined type microbial fertilizer to promote the growth of kiwifruits; three continuous pot experiments show that SCAU-1 fermentation liquor has an obvious growth-promoting effect for kiwifruit seedlings by using a root-irrigation way after being diluted by clear water, so that the root numbers, root lengths, plant heights, leaf numbers and leaf areas during the kiwifruit seedling stage can be obviously improved.
Description
Technical field
The invention belongs to microbial technology field, more particularly to a kind of separation and its application of Fructus actinidiae chinensiss endogeny rayungus.
Background technology
How delicious Kiwi berry nutritious non-lipid, juice is, and by people " king of fruit " is described as, and is preferable pollution-free food, in recent years
Demand increasingly increases.But it is because that orchard worker pursues simply high yield, using unscientific cultivation management mode, the such as weight of nitrogen phosphorus fertilizer
Apply, potash fertilizer is gently applied, scarce apply etc. of fertilizer causes the Fructus actinidiae chinensiss to be susceptible to suffer from the diseases such as Peptic Ulcerss, yellowing leaf disease, causes Fructus actinidiae chinensiss
Percent rate of high quality fruit declines, soil fertility is rapidly cut down, and has a strong impact on Fructus actinidiae chinensiss yield and quality.
Microbial manure can effective improved soil fertility, the utilization rate for improving chemical fertilizer improves energy utilization rate simultaneously.
It was verified that microbial manure is in green organic food production, Agriculture Ecological Environment Protection and an agriculture featuring high yields, fine quality and high efficiency
Sustainable development in play an important role.The leitungskern of microbial manure is beneficial microbe, after being manured into soil, can be solid
Determine the nitrogen in air, the nutrient in activating soil improves the nutrient environment of plant, and microorganism is produced during vital movement
Active substance, stimulates the growth of plant.But what current microbial manure generally existing single function, strain activity were degenerated asks
Topic, therefore, excavate new functional microorganism significant for the research and development of multifunctional microbial fertilizer.
Endophyte is the microorganism that the obvious disease of plant was lived in living plant tissue, will not caused to a class, can conduct
The carrier of exogenous gene, with promote plant growing, degeneration-resistant border, disease resistance, increase host plant allelopathy and to disease
Preventing and treating of insect pest etc. is acted on.Fructus actinidiae chinensiss root endophyte enriches, and ill plant can induce the micropopulation of itself root to shave one's head
Raw response change, to suffer from the kiwi fruit plant root of Peptic Ulcerss as separation material, can improve the work(with potential disease-resistant growth-promoting
The separation efficiency of energy microorganism.To suffer from the kiwi fruit plant root of Peptic Ulcerss as separation source, the bolter of endogeny rayungus is carried out
Select the microbial resources with disease-resistant growth-promoting complex function new for excavation and be applied to Novel compound microbial agent
Exploitation it is significant.
The content of the invention
It is an object of the invention to provide a kind of Fructus actinidiae chinensiss endogeny rayungus and its application, by overall merit macaque is screened
The actinomycetic functional characteristic in Fructus Persicae inside, it is intended to solve single present microorganism fertilizer production strain, the degeneration of bacterial strain merit, Mi
The problems such as monkey Fructus Persicae special microorganism microbial inoculum environmental suitability is weak.
For achieving the above object, the technical solution used in the present invention is：A kind of Fructus actinidiae chinensiss root endogeny rayungus, the Mi
Monkey Radix Persicae portion endogeny rayungus are styreptomyces globispotus strain, are named as styreptomyces globispotus strain SCAU-1 (Streptomyces
Globisporus SCAU-1), in the preservation of October 18 in 2016 to Wuhan University's China typical culture collection center
(CCTCC), deposit number is：CCTCC NO：M 2016577.The bacterium has wide antibacterial action, while plant can be promoted
Growth, has " fertile medicine " economic benefits and social benefits function concurrently, fertile for chemistry is reduced in agricultural planting industry using microbial manure made by the bacterium
Material and the applications of pesticide are significant.
Another object of the present invention is to a kind of separation method of Fructus actinidiae chinensiss root endogeny rayungus, gathers and suffers from Peptic Ulcerss
Fructus actinidiae chinensiss root samples, with 0.1%Tween 20 30s, aseptic water washing 3 times, 75% ethanol immersion 5min, sterilized water punching are cleaned
Wash 3 times, the liquor natrii hypochloritises of available chlorine content 2% immersion 3-5min, aseptic water washing 3 times；10%NaHCO3Immersion 10min,
Sterile water wash 3 times, is then ground to homogenate and is followed by TSA culture medium flat plates, causes to cultivate 30d at 28 DEG C, according to bacterium colony
The appearance features picking single bacterium colony such as size, form and color and luster simultaneously carries out line purification, each by separating 32 plants of colonial morphologies of acquisition
The bacterial strain for differing.
Further, the biological property of the bacterial strain is as follows：The bacterial strain is to staphylococcus aureuses, escherichia coli, Flos Caryophylli
The withered and yellow pathogenic bacteria of pseudomonass, Rhizoctonia solani Kahn, Citrullus vulgariss and tomato early blight bacterium are inhibited, and IAA yield is more than 40mg/
L, can produce cellulase.
Another object of the present invention is to fermentation prepared by Fructus actinidiae chinensiss endogeny rayungus described in a kind of utilization claim 1
Liquid.
Further, including：Gause I culture fluid is inoculated in after the styreptomyces globispotus strain SCAU-1 activation that screening is obtained
In, 30 DEG C, cultivate in the constant-temperature table of 120r/min, after culture 96h, as seed liquor with 1:1 ratio adds Gause I training
Foster base, 30 DEG C, 96h is cultivated in the constant-temperature table of 120r/min makes its viable count reach more than 1,000,000,000, takes out standby.
Another object of the present invention is to styreptomyces globispotus strain SCAU-1 bacterium prepared by a kind of utilization Fructus actinidiae chinensiss endogeny rayungus
Liquid bacterial agent.
Another object of the present invention is to a kind of application of Fructus actinidiae chinensiss endogeny rayungus fermentation liquid in Fructus actinidiae chinensiss plantation.
Further, when Fructus actinidiae chinensiss are transplanted, SCAU-1 will be entered by 50-200 dilution proportion SCAU-1 bacterial strain fermentation liquors
Row Fructus actinidiae chinensiss root irrigation, viable bacteria content is about 2 × 107cfu/ml.
Application of the fermentation liquid of the present invention in Fructus actinidiae chinensiss are potted plant, will press the fermentation of the SCAU-1 bacterial strains of 50-200 dilution proportions
Liquid is carried out the bacterium that connects of macaque peach seedling by the way of pouring root and is processed.
The method have the benefit that：Fructus actinidiae chinensiss endogeny rayungus and its application that the present invention is provided, from Fructus actinidiae chinensiss
In isolate special endogeny rayungus, the growth of plant not only can be promoted, plant pathogenic fungi and antibacterial can also be suppressed
Growth, and possess highly effective cellulose degradation capability, can be used for the making of disease-resistant growth-promoting type multifunctional microbial fertilizer, improve soil
Physicochemical property, promotes plant growing；Continuous three potted plant experiments show SCAU-1 fermentation liquids Jing after clear water dilution, using pouring root
Mode shows obvious growth-promoting functions to Fructus actinidiae chinensiss, can significantly improve radical, root length, plant height, the blade in Fructus actinidiae chinensiss seedling stage
Quantity and leaf area.The styreptomyces globispotus strain SCAU-1 of the present invention, with wide antibacterial action, while plant can be promoted to give birth to
It is long, with " fertile medicine " and effect function, using fermenting agent made by the bacterium for reduce in agricultural planting industry chemical fertilizer and
The applications of pesticide are significant；Bacterial strain has extensive antibacterial activity, highly effective cellulose degradation capability, while IAA is produced, it is right
Plant has and significantly promote growth, shows multi-biological function.The bio-feritlizer that the bacterium makes can promote Mi
The growth of the plants such as monkey Fructus Persicae, while promoting quality of Chinese gooseberries.For in Kiwifruit Culture carry out " fat-reducing subtracts medicine " have it is potential
Using value.
Description of the drawings
In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
The accompanying drawing to be used needed for having technology description is briefly described, it should be apparent that, drawings in the following description are only this
Some embodiments of invention, for those of ordinary skill in the art, without having to pay creative labor, may be used also
To obtain other accompanying drawings according to these accompanying drawings.
Fig. 1 is the preparation method flow chart of Fructus actinidiae chinensiss endogeny rayungus provided in an embodiment of the present invention；
Fig. 2 is comparison diagram before and after Fructus actinidiae chinensiss potted plant experiment provided in an embodiment of the present invention.
Specific embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, the technical scheme in the embodiment of the present invention is carried out clear, complete
Site preparation is described, it is clear that described embodiment is only a part of embodiment of the invention, rather than the embodiment of whole.It is based on
Embodiment in the present invention, it is every other that those of ordinary skill in the art are obtained under the premise of creative work is not made
Embodiment, belongs to the scope of protection of the invention.
The Fructus actinidiae chinensiss endogeny rayungus of the embodiment of the present invention, Jing forms, physiology, Molecular Identification are styreptomyces globispotus strain
(Streptomyces globisporus), is named as styreptomyces globispotus strain SCAU-1 (Shreptamyccs glablsporus
SCAU-1), in the preservation of October 18 in 2016 to Wuhan University's China typical culture collection center (CCTCC), preservation is compiled
Number it is：CCTCC NO：M 2016577.
As shown in figure 1, the preparation method of the Fructus actinidiae chinensiss endogeny rayungus of the embodiment of the present invention is comprised the following steps：
S101：Fructus actinidiae chinensiss are selected, and pick up from Sichuan tradition Fructus actinidiae chinensiss main producing region-Dujiang weir, and plant suffers from Peptic Ulcerss；
S102：The separation of Fructus actinidiae chinensiss root endogeny rayungus, to suffer from Peptic Ulcerss Fructus actinidiae chinensiss root as separation material, root is cut
It is short into 1cm or so, using 75% ethanol and 2% sodium hypochlorite surface sterilization.Grind to form and coat after homogenate Gause I training
In foster base flat board, compared with last time washings, to detect whether surface sterilization is thorough；
S103：Cause to cultivate 30d at 28 DEG C, according to the appearance features picking such as bacterium colony size, form and color and luster single bacterium colony simultaneously
Carry out line purification.By separating, the different bacterial strain of 32 plants of colonial morphologies is obtained altogether.
The application principle of the present invention is further described with reference to specific embodiment.
(1) styreptomyces globispotus strain (Streptomyces globisporus) SCAU-1 bacterial strain screenings
1st, Fructus actinidiae chinensiss are selected
Sichuan tradition Fructus actinidiae chinensiss planting base-Dujiang weir is picked up from, Fructus actinidiae chinensiss suffer from Peptic Ulcerss；
2nd, the separation of Fructus actinidiae chinensiss endogeny rayungus
Using suffering from Peptic Ulcerss Fructus actinidiae chinensiss root samples, with 0.1%Tween 20 30s is cleaned, aseptic water washing 3 times, 75%
Ethanol soaks 5min, aseptic water washing 3 times, the liquor natrii hypochloritises of available chlorine content 2% immersion 3-5min, aseptic water washing 3 times；
10%NaHCO3Immersion 10min, sterile water wash 3 times.It is then ground to homogenate to be followed by TSA culture medium flat plates, with last
Once washing water is compared, to detect whether surface sterilization is thorough；Cause at 28 DEG C cultivate 30d, according to bacterium colony size, form and
The appearance features picking single bacterium colony such as color and luster simultaneously carries out line purification.By separating, 32 plants of colonial morphologies are obtained altogether different
Bacterial strain.
3rd, endogeny rayungus produce heteroauxing (IAA) and determine
By 32 plants of endogeny rayungus cultures in step 2 to logarithmic (log) phase, the μ l of bacterium solution 50 are taken in the cattle containing 0.5g/L tryptophans
In meat extract fluid medium in (about 3ml), it is repeated 3 times, 28 DEG C of 140r/min shaken cultivation 1.5 days, takes 50 μ l white in what is cleaned
In porcelain plate, plus 100 μ l developers, lucifuge 30min at 25 DEG C, if there is pink, show to produce IAA, not connect bacterium
Carnis Bovis seu Bubali cream fluid medium containing 0.5g/L tryptophans is control.
The endogeny rayungus for producing heteroauxing are continued to cultivate in the Carnis Bovis seu Bubali cream fluid medium containing 0.5g/L tryptophans
After 24-48h, 8000r/min centrifugation 5min take supernatant 2ml and add 4ml Salkowski developers, lucifuge 30min at 25 DEG C
Afterwards, mensuration absorbance value under 530nm.Compared with blank cultures, and standard is done with the corresponding optical density of the standard sample of IAA
Curve, calculates the yield (mg/L) of IAA.
There are 19 plants of generation IAA in 32 plants of bacterium, bacterial strain of the yield more than 40mg/L there are 3 plants, wherein SCAU-1 bacterial strains IAA yield
Highest, is 43.3mg/L.
4th, cellulase-producing is determined
By the bacterial strain dibbling in step 2 on sodium carboxymethyl cellulose culture medium flat plate, 30 DEG C of culture 2d, with 1mg/mL's
Congo red solution infects 1h, then with the NaCl solution decolouring 1h of 1mol/L, and pouring the HCl of 1mol/L into makes background in blue and make
Bright circle becomes apparent from, and records transparent loop diameter HD values with colony diameter CD values and calculates its ratio.There are 3 plants of tool fibers in 32 plants of bacterium
Plain enzyme activity, the HD/CD values about 1.45 of SCAU-1.
6th, bacterial strain bacteriostasis are determined
With staphylococcus aureuses, escherichia coli, pseudomonas syringae, Rhizoctonia solani Kahn, the withered and yellow pathogenic bacteria of Citrullus vulgariss and kind
Eggplant early epidemic pathogenic bacteria is, for examination pathogen, by flat board antagonism inhibitory action of the isolated strains to pathogen to be determined.Record HD values and
CD values simultaneously calculate its ratio.32 plants supply in examination endogeny rayungus have 6 plants to show inhibition to above-mentioned 6 kinds of pathogen, its
Middle SCAU-1 is respectively 2.4 and 3.2 to the withered and yellow pathogenic bacteria of Citrullus vulgariss and colibacillary HD/CD values, to staphylococcus aureuses, Flos Caryophylli
The HD/CD values of pseudomonass, Rhizoctonia solani Kahn and tomato early blight bacterium are between about 1.2-2.1.
7th, the preparation of SCAU-1 fermentation liquids
Styreptomyces globispotus strain (Streptomyces globisporus) the SCAU-1 activation for obtaining will be screened in above-mentioned steps
After be inoculated in Gause I culture fluid, 30 DEG C, in the constant-temperature table of 120r/min cultivate, culture 36h after, as seed liquor with
1:1 ratio adds No. bis- culture medium of Gao Shi, and 30 DEG C, 36h is cultivated in the constant-temperature table of 120r/min makes its viable count reach 1,000,000,000
More than, take out standby.
8th, the potted plant application of Fructus actinidiae chinensiss
It is Red Male for trying Kiwifruit Cultivars, the fermentation liquid of the SCAU-1 bacterial strains for diluting by a certain percentage is adopted into the side of pouring root
Formula carries out the bacterium that connects of macaque peach seedling and processes, and is compared with sterilized water, and macaque peach seedling index of correlation is determined after culture 60.
The macaque peach seedling of table 1 throughout manage in morphological index
As it can be seen from table 1 the fermentation liquid dilution of variable concentrations SCAU-1 bacterial strains to the radical of macaque peach seedling, root length,
Plant height, the number of blade and chlorophyll content show facilitation, with potential using value.
Those skilled in the art of the present technique are appreciated that unless otherwise defined all terms used herein are (including technology art
Language and scientific terminology) have with art of the present invention in those of ordinary skill general understanding identical meaning.Should also
It is understood by, those terms defined in such as general dictionary should be understood that the meaning having with the context of prior art
The consistent meaning of justice, and unless defined as here, will not be with idealizing or excessively formal implication is explaining.
It should be noted last that：Above example is only to illustrative and not limiting technical scheme, although ginseng
The present invention has been described in detail according to above-described embodiment, it will be apparent to an ordinarily skilled person in the art that：Still can be to this
Invention is modified or equivalent, any modification or partial replacement without departing from the spirit and scope of the present invention, and its is equal
Should cover in the middle of scope of the presently claimed invention.
Claims (10)
1. a kind of Fructus actinidiae chinensiss root endogeny rayungus, it is characterised in that the Fructus actinidiae chinensiss root endogeny rayungus are ball spore strepto-
Bacterium, is named as styreptomyces globispotus strain SCAU-1, and deposit number is：CCTCC NO：M 2016577.
2. a kind of separation method of Fructus actinidiae chinensiss root endogeny rayungus as claimed in claim 1, it is characterised in that ulcer is suffered from collection
The Fructus actinidiae chinensiss root samples of disease, with 0.1%Tween 20 30s is cleaned, and aseptic water washing 3 times, 75% ethanol immersion 5min is aseptic
Water is rinsed 3 times, the liquor natrii hypochloritises of available chlorine content 2% immersion 3-5min, aseptic water washing 3 times；10%NaHCO3Immersion
10min, sterile water wash 3 times is then ground to homogenate and is followed by TSA culture medium flat plates, causes to cultivate 30d, root at 28 DEG C
According to the appearance features picking single bacterium colony such as bacterium colony size, form and color and luster and carry out line purification, by separating 32 plants of bacterium colonies are obtained
The different bacterial strain of form.
3. Fructus actinidiae chinensiss root endogeny rayungus according to claim 1 or claim 2, it is characterised in that the biological property of the bacterial strain
It is as follows：The bacterial strain to staphylococcus aureuses, escherichia coli, pseudomonas syringae, Rhizoctonia solani Kahn, the withered and yellow pathogenic bacteria of Citrullus vulgariss and
Tomato early blight bacterium is inhibited, and IAA yield is more than 40mg/L, can produce cellulase.
4. the fermentation liquid that prepared by Fructus actinidiae chinensiss endogeny rayungus described in a kind of utilization claim 1.
5. the preparation method of fermentation liquid according to claim 4, it is characterised in that include：The ball spore strepto- that screening is obtained
It is inoculated in Gause I culture fluid after bacterium SCAU-1 activation, 30 DEG C, cultivates in the constant-temperature table of 120r/min, after culture 96h,
As seed liquor with 1:1 ratio adds Gause I culture medium, and 30 DEG C, 96h is cultivated in the constant-temperature table of 120r/min makes it live
Bacterium number reaches more than 1,000,000,000, takes out standby.
6. the styreptomyces globispotus strain SCAU-1 bacteria liquid microbial inoculums that prepared by Fructus actinidiae chinensiss endogeny rayungus described in a kind of utilization claim 1.
7. application of a kind of fermentation liquid as claimed in claim 4 in Fructus actinidiae chinensiss plantation.
8. application according to claim 7, it is characterised in that will by 50-200 dilution proportion SCAU-1 bacterial strain fermentation liquors,
When Fructus actinidiae chinensiss are transplanted, SCAU-1 is carried out into Fructus actinidiae chinensiss root irrigation, viable bacteria content is about 2 × 107cfu/ml.
9. application according to claim 7, it is characterised in that the fermentation of the SCAU-1 bacterial strains of 50-200 dilution proportions will be pressed
Liquid is carried out the bacterium that connects of macaque peach seedling by the way of pouring root and is processed.
10. application of the Fructus actinidiae chinensiss root endogeny rayungus in agricultural planting according to claim 1.
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| CN111197016A (en) * | 2020-01-10 | 2020-05-26 | 渭南职业技术学院 | Screening method of kiwifruit canker antagonistic strain |
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