CN104894018A - Luna Lin Haresii streptomycete and application thereof - Google Patents
Luna Lin Haresii streptomycete and application thereof Download PDFInfo
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Abstract
The invention relates to the technical field of biology, and discloses Luna Lin Haresii streptomycete and an application thereof. The Luna Lin Haresii streptomycete is obtained by separating and screening a banana garden soil sample of a blight area, is named Luna Lin Haresii B-03 (Streptomyces Lunalinharesii) and is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC No. M2015148. The Luna Lin Haresii streptomycete has a function for inhibiting pathogenic bacteria such as fusarium oxysporum, gloeosporium musarum, banana long leaf spot disease, banana big greyness disease, banana pseudocercosporella herpotrichoides, banana leaf margin blight disease and litchi colletotrichum; a strain fermentation solution of the Luna Lin Haresii streptomycete also has a remarkable antibacterial effect.
Description
Technical field
The present invention relates to biological technical field, refer to a kind of Lu Nalinrui streptomycete and application thereof especially.
Background technology
Streptomyces (streptomyces), its substrate mycelium multi-branched, general tabula is sparse, seldom ruptures, the various water-soluble or fat-soluble pigment of normal generation; Aerial hyphae is slightly thicker than substrate mycelium, and by epitheca is wrapped, aerial hyphae partial differentiation becomes straight shape, flexible, hook and loop is spacious to pine or the fibrillae of spores of tight spiral, and often containing the spore of about 50, short person is containing 5 ~ 10 spores; Spore is arthrospore, and being ruptured by mycelia forms, and what have sloughs epitheca, and what have carries epitheca or remnants, and epitheca determines the surface tissue of spore; G+C mol content in DNA is 69 ~ 76%.This genus strain number is maximum, and taxonomic identification is more difficult, and it is generally acknowledged the surface tissue of the shape of fibrillae of spores, spore, the color of spore and in organic substratum, whether produce Melanoidins is topmost classification indication.Their distributed poles in soil are wide, and riotous growth on artificial medium mostly, minority is plant pathogen.
Banana is Musaceae Musa per nnial herb, is one of the torrid zone, subtropics main fruit.Because banana cultural area is extensive, cause the outburst of multiple disease and pest, as banana blight, banana anthracnose, leaf spot etc., wherein especially serious with banana blight harm, once large-scale outbreak, bring destructive strike can to the cultivation of local banana.Banana blight is a kind of soil-borne disease caused by fungal infection.This pathogenic bacteria is Fusarium oxysporum, is a kind of soil inhabitant, soil disinfection, and the quantity reducing pathogenic bacteria in soil is one of important means that control banana is withered.And this research is screened by microorganism in the Banana soil to banana blight region of disease, to the bacterial strain of the banana blight bacteria that is inhibited.
Summary of the invention
The present invention proposes a kind of streptomycete and application thereof, and all have restraining effect to multiple pathogenic bacteria, fungistatic effect is remarkable.
First aspect of the present invention provides a kind of Lu Nalinrui streptomycete, called after Lu Nalinrui streptomycete (Streptomyces lunalinharesii) B-03, China typical culture collection center is preserved on March 23rd, 2015, address is in the Wuhan University of Wuhan City, Hubei Province, and deposit number is CCTCC NO:M2015148.
The 16S rRNA gene order of described Lu Nalinrui streptomycete B-03 is as shown in SEQ ID NO.1.
Second aspect of the present invention is to provide the application of Lu Nalinrui streptomycete B-03 in preparation control banana blight bacteria preparation described in the present invention first aspect.
3rd the aspect of the present invention Lu Nalinrui streptomycete B-03 be to provide described in the present invention first aspect suppresses the application of the preparation of musae, the microscler leaf spot of banana, the large graywall of banana, tribute any of several broadleaf plants eye spot, banana leaf margin blight, lichee anthrax-bacilus in preparation.
4th aspect of the present invention is to provide a kind of Lu Nalinrui streptomycete bacterial strain fermented liquid, Lu Nalinrui streptomycete B-03 described in the present invention first aspect is inoculated in after soyflour fermention medium 28 DEG C cultivates 3 ~ 5d and obtains fermentation seed liquid, described fermentation seed liquid is inoculated in soya-bean cake substratum, 28 DEG C ~ 30 DEG C, 180rpm shakes cultivation 7 ~ 10d, obtains described bacterial strain fermentation liquor.
Further, described soyflour cultivate and composition, be designated as by weight: soyflour 5 ~ 10 parts, NaCl1 ~ 5 part, CaCO3 1 ~ 3 part, peptone 2 ~ 5 parts, glucose 5 ~ 10 parts; Described soyflour Medium's PH Value is 7.0 ~ 7.5; The composition of described soya-bean cake fermention medium, is designated as by weight: 180 ~ 200 parts, brown sugar, soya-bean cake 50 ~ 70 parts, 800 ~ 1200 parts, water.
5th aspect of the present invention is to provide a kind of Lu Nalinrui streptomycete bacterial strain fermented liquid suppresses Fusarium oxysporum No. 1 microspecies, Fusarium oxysporum No. 4 microspecies and colletotrichum gloeosporioides Penz preparation application in preparation.
6th aspect of the present invention is to provide a kind of Lu Nalinrui streptomycete bacterial strain fermented liquid suppresses banana blight preparation application in preparation
Further, after the bacterial strain fermentation liquor dilution described in the present invention the 6th aspect, directly field crop is applied to.
Further, described bacterial strain fermentation liquor dilution 50 ~ 100 times fills with root afterwards or ground sprays.
Lu Nalinrui streptomycete bacterial strain of the present invention, through multinomial qualification, be defined as Lu Nalinrui streptomycete (Streptomyces lunalinharesii) B-03, and carried out culture presevation on March 23rd, 2015 in China typical culture collection center (CCTCC), and prove survival, preservation address is in Wuhan University of Wuhan City of Hubei China province, and deposit number is CCTCC NO:M2015148.
Beneficial effect of the present invention:
Lu Nalinrui streptomycete B-03 of the present invention, has good restraining effect to banana blight bacteria, and the bacterial strain fermentation liquor of preparation is applied in potted plant banana seedlings, has significant control effect to banana blight.In addition, chain Lu Nalinrui streptomycete of the present invention also has good restraining effect to pathogenic bacterias such as musae, the microscler leaf spot of banana, the large graywall of banana, tribute any of several broadleaf plants eye spot, banana leaf margin blight and lichee anthrax-bacilus simultaneously, there is antibacterial effect widely, for the Synthetical prevention of Plant diseases, especially the Synthetical prevention of disease multiple, occurred frequently in banana cultivation, is significant.
Accompanying drawing explanation
In order to be illustrated more clearly in the embodiment of the present invention or technical scheme of the prior art, be briefly described to the accompanying drawing used required in embodiment or description of the prior art below, apparently, accompanying drawing in the following describes is only some embodiments of the present invention, for those of ordinary skill in the art, under the prerequisite not paying creative work, other accompanying drawing can also be obtained according to these accompanying drawings.
Fig. 1 is the growth conditions picture of Lu Nalinrui streptomycete B-03 on different culture media;
In figure, ISP1 is Gause I substratum; ISP2 is yeast extract paste malt extract agar substratum; Rolled oats ISP3 is nutrient agar; ISP4 is inorganic salt starch agar medium; ISP5 is glucose-asparagine nutrient agar; ISP6 is peptone-yeast extract agar substratum; ISP7 tyrosine agar substratum.
Fig. 2 is that Lu Nalinrui streptomycete B-03 detects picture to tribute any of several broadleaf plants eye spot bacterium inhibit activities;
Fig. 3 is that Lu Nalinrui streptomycete B-03 detects picture to lichee anthrax bacteria inhibit activities;
Fig. 4 is that Lu Nalinrui streptomycete B-03 detects picture to the large Cercospora Sojina Hara inhibit activities of banana;
Fig. 5 is that Lu Nalinrui streptomycete B-03 detects picture to banana blight bacteria inhibit activities;
Fig. 6 is that Lu Nalinrui streptomycete B-03 detects picture to banana leaf margin wilt inhibit activities;
Fig. 7 is that Lu Nalinrui streptomycete B-03 detects picture to the microscler leaf spot fungi inhibit activities of banana;
Fig. 8 is that Lu Nalinrui streptomycete B-03 detects picture to musae inhibit activities;
In Fig. 2 ~ Fig. 8, a is Lu Nalinrui streptomycete B-03 bacterial strain, and b is tribute any of several broadleaf plants eye spot germ, c is lichee anthrax bacteria, and d is the large Cercospora Sojina Hara of banana, and e is banana blight germ, f is banana leaf margin wilt, and g is the microscler leaf spot fungi of banana, and h is musae.
Fig. 9 adopts bioautography tlc to detect Lu Nalinrui streptomycete B-03 fermented liquid stoste to the restraining effect picture of various pathogenic bacteria.
In Fig. 9, A is colletotrichum gloeosporioides Penz, and B is Fusarium oxysporum No. 1 microspecies, and C is Fusarium oxysporum No. 4 microspecies.
Embodiment
Below in conjunction with the accompanying drawing in the embodiment of the present invention, be clearly and completely described the technical scheme in the embodiment of the present invention, obviously, described embodiment is only the present invention's part embodiment, instead of whole embodiments.Based on the embodiment in the present invention, those of ordinary skill in the art, not making the every other embodiment obtained under creative work prerequisite, belong to the scope of protection of the invention.
One, the screening of bacterial strain
Jiao Yuan respectively at blight region of disease, Nan Baozhen Xin Ying farm, Hainan Province gets pedotheque.Sampling method is that each field adopts 5 random sampling to be mixed into a sample, gathers banana plant rhizosphere soil (10cm ~ 30cm soil layer).The fresh sample gathered from field puts into plastic sample collection bag, and is placed in ice chest, takes back laboratory and is placed in 4 DEG C of refrigerators and saves backup.
Adopt gradient dilution method that above-mentioned pedotheque is made 10
-2~ 10
-4concentration gradient series suspension, actinomycetic separation, adopts Gause I substratum (will add the growth of 3% potassium bichromate anti-bacteria, fungi before use); The separation of bacterium, adopts LB substratum and 10
-4~ 10
-6extent of dilution.The dilution solution 0.1mL of above-mentioned difference is drawn respectively with pipettor, drip on culture medium flat plate, even with the coating of L-type glass rod, slightly dry, culture dish is inverted, in 25 ~ 28 DEG C of incubators, cultivate 2 ~ 5d, chooses carry out purifying cultivation according to the bacterium of the color of the bacterium colony that flat board grows, form, size and actinomycetic single bacterium colony to new substratum, bacterial strain after purifying is numbered respectively, preserves and record the number of strains that different sample separation arrives.
Adopt dull and stereotyped opposite culture method, detect the above-mentioned various bacteriums be separated to, actinomycetes strain (hereinafter referred to as " test strains ") is to the bacteriostatic activity of Fusarium oxysporum No. 4 physiological strains (hereinafter referred to as " target bacterial strain "), activate with punch tool (diameter 6mm) in advance 3d and cultivated target bacterial strain colony edge and cut mycelia block, and switching is in the dull and stereotyped central authorities of another PDA, again the test strains after activation is seeded in apart from target bacterial strain mycelia block 2 ~ 3cm place, every plating four test strains, not take over the target bacterial strain of what test strains for contrast, each bacterial strain repeats for three times.After 25 ~ 28 DEG C of constant temperature culture for some time, measure the antibacterial bandwidth between test strains and target bacterial strain, filter out bacterial strain B-03 Fusarium oxysporum No. 4 physiological strains to antagonistic action.
LB substratum forms: the old 10.0g of Trypsin, yeast powder 5.0g, NaCl 10.0g, agar 17 ~ 20.0g, water 1000.0mL, with 1mol/L NaOH adjust pH to 7.5.What do not add agar is LB nutrient solution.
PDA substratum forms: potato 200.0g, glucose 20.0g, agar powder 17 ~ 20.0g, water 1000.0mL.
Two, the qualification of bacterial strain
1. the 16S rDNA gene order of bacterial strain B-03 is as shown in SEQ ID NO.1.
2. the 16S rDNA gene sequencing BLAST result of bacterial strain B-03 is as shown in table 1 below.
The 16S rDNA gene sequencing BLAST result of table 1 bacterial strain B-03
3. the evolutionary tree of bacterial strain B-03
4. the growing state of bacterial strain B-03 and form
At the different substratum streptomycete bacterial strain that obtains of culture of isolated respectively, compare its cultural colony, as shown in table 2, bacterial strain different culture media cultural colony as shown in Figure 1, under bacterial classification microscope (1000X), photo is as illustrated in figs. 2-7.Substratum is according to following formulated.
Gause I substratum (ISP1): Zulkovsky starch 20.0g, NaCl 0.5g, KNO
31.0g, K
2hPO
43H
2o 0.5g, MgSO
47H
2o 0.5g, FeSO
47H
2o 0.01g, agar 15-20g, H
2o 1000mL.Soyflour fermention medium: soyflour 10.0g, NaCl 2.5g, CaCO
32.0g, peptone 3.0g, glucose 10.0g, pH 7.2 ~ 7.4.
Yeast extract paste malt extract agar substratum (ISP2): yeast extract paste 10g, Fructus Hordei Germinatus leaching powder 10g, glucose 4g, pH 7.3.Oatmeal agar substratum (ISP3): oatmeal 20g, Trace salts solution 1mL.
Inorganic salt starch agar medium (ISP4): starch 10g, K
2hPO
41g, MgSO
47H
2o 1g, NaCl1g.
Glucose-asparagine nutrient agar (ISP5): asparagine 10g, glycerine 10g, K
2hPO
41g, trace salt 1mL, pH 7.0-7.2.
Peptone-yeast extract agar substratum (ISP6): peptone 15g, show peptone 5g, ferric ammonium citrate 0.5g, dipotassium hydrogen phosphate 1g, Sulfothiorine 0.08g, yeast extract paste 1g, distilled water 1L, agar 20g, pH7.0-7.4.
Tyrosine agar substratum (ISP7): glycerine 15g, tyrosine 0.5g, aspartoyl ammonium 1g, K
2hPO
40.5g, MgSO
47H
2o 0.5g, NaCl 0.5g, ferrous sulfate 0.01g, Trace salts solution 1mL, pH 7.2-7.4.Trace salts solution: FeSO
47H
2o 0.1g, MnCl
24H
2o 0.1g, ZnSO
47H
2o 0.1g, distilled water 100mL.
Table 2 different culture media strain culturing proterties is added up
As shown in Table 2, bacterial strain B-03 all can grow on above-mentioned 7 kinds of substratum, and above-mentioned 7 kinds of substratum do not produce soluble pigment.
5. the utilization of carbon source of bacterial strain B-03 is as shown in table 3 below.
Table 3 bacterial strain B-03 utilization of carbon source situation
+: positive ,-: negative, w: the weak positive
Comprehensive above detected result, bacterial strain B-03 is accredited as Lu Nalinrui streptomycete (Streptomyces lunalinharesii), and on March 23rd, 2015, this bacterial strain is carried out preservation in China typical culture collection center, and proving survival, deposit number is: CCTCC NO:M 2015148.
Three, Lu Nalinrui streptomycete B-03 bacteriostatic activity detects
Adopt dull and stereotyped opposite culture method, test Lu Nalinrui streptomycete B-03 is to the bacteriostatic activity of pathogenic fungi.First by the Lu Nalinrui streptomycete B-03 inoculation after activation apart from pathogenic bacteria mycelia block 2 ~ 3cm place, every plating four Lu Nalinrui streptomycete B-03 bacterial strains, not take over the pathogenic bacteria of He Lunalinrui streptomycete B-03 bacterial strain for contrast, each bacterial strain repeats for three times, after 25 ~ 28 DEG C of constant temperature culture 3d, measure the index of the antibacterial bandwidth between Lu Nalinrui streptomycete B-03 bacterial strain and pathogenic fungi bacterial strain as bacteriostatic action power.Antibacterial detected result is as shown in Fig. 2 to Fig. 8, and antibacterial bandwidth is as shown in table 4.
Table 4 Lu Nalinrui streptomycete B-03 is to the restraining effect of different strains
As shown in Table 4, Lu Nalinrui streptomycete B-03 all has restraining effect in various degree to above-mentioned pathogenic bacteria, all can reach restraining effect to common banana pathogenic bacteria, wherein best to banana microscler leaf spot inhibition, is secondly banana blight germ.For Synthetical prevention Plant diseases, especially multiple, the occurred frequently disease of banana, has certain using value.
Four, the preparation of Lu Nalinrui streptomycete bacterial strain fermented liquid
Inclined-plane Lu Nalinrui streptomycete B-03 bacterial strain activation culture on YE substratum of first will preserve, culture temperature is 28 DEG C, by the inoculation after activation in soyflour fermention medium: soyflour 5 ~ 10g, NaCl1 ~ 5g, CaCO
31 ~ 3g, peptone 2 ~ 5g, glucose 5 ~ 10g; Described soyflour Medium's PH Value is obtain fermentation seed liquid after 7.0 ~ 7.5,28 DEG C of cultivation 3 ~ 5d; Above-mentioned seed liquor be inoculated in soya-bean cake fermention medium, inoculum size gets fermentation seed liquid according to 10% of soya-bean cake fermention medium volume; Described soya-bean cake culture medium prescription is: brown sugar 180 ~ 200g, soya-bean cake 50 ~ 70g, water 800 ~ 1200g, 28 DEG C ~ 30 DEG C, and 180rpm shakes cultivation 7 ~ 10d, obtains bacterial strain fermentation liquor.Above-mentioned fermented liquid can dilute and be directly used in field crop.During application field crop, can spray with liquid manure pipeline filling root or ground.
YE substratum consists of: glucose 4g, yeast powder 4g, malt meal 10g, water 1000ml, PH7.2-7.4.Five, Lu Nalinrui streptomycete bacterial strain fermented liquid is to the restraining effect of pathogenic bacteria
Adopt thin layer chromatography board (silica-gel plate) to detect the bacteriostatic activity of Lu Nalinrui streptomycete B-03 bacterial strain fermentation liquor, and adopt bioautography tlc to detect the size of inhibition zone.Concrete operations are: by the Lu Nalinrui streptomycete B-03 bacterial strain fermentation liquor stoste 4ul and 8ul obtained, be numbered D1-F4 and D1-F8 respectively, adopt kapillary respectively o'clock to 2 silica-gel plate central authorities, air-dry rear for subsequent use; Fermented liquid stoste is adopted freeze drier freeze-drying, obtain dry sample, the dry sample 0.5g obtained is dissolved in 1ml ethanol, after abundant dissolving, get 4ul and 8ul, be numbered D1-DF4 and D1-DF8 respectively, adopt aforesaid method respectively o'clock to 2 silica-gel plate central authorities, the conidial suspension of different pathogenic bacterias is dissolved in the PDB of 50% agar concentration respectively, and spore final concentration is adjusted to 1.0x 10
6cFU, is evenly sprayed on the silica-gel plate of prior point sample by different pathogenic bacteria conidiums respectively, and moisturizing also in illumination box 25 DEG C, is cultivated and observed antibacterial circle diameter after 4 days.As described in Table 5, bacterial strain fermentation liquor stoste to three kinds of pathogenic bacteria restraining effect as shown in Figure 9.
Table 5 Lu Nalinrui streptomycete B-03 bacterial strain fermentation liquor is to the restraining effect of fungi
As can be seen from Table 5, Lu Nalinrui streptomycete B-03 bacterial strain fermentation liquor all has good inhibit activities to three kinds of experimental strains, and when bacterial strain fermentation liquor concentration is identical, fermenation raw liquid bacteriostatic activity is more more remarkable than the fermented liquid bacteriostatic activity of freeze-drying.Illustrate by frozen dried, have impact on the bacteriostatic activity of Lu Nalinrui streptomycete B-03 bacterial strain fermentation liquor, therefore, for preventing and treating pathogenic bacteria, prioritizing selection Lu Nalinrui streptomycete B-03 bacterial strain fermentation liquor stoste.
Six, Lu Nalinrui streptomycete bacterial strain fermented liquid is to the prevention effect of potted plant banana seedlings blight
Pot experiment detects Lu Nalinrui streptomycete bacterial strain fermented liquid to the prevention effect of banana blight, and the condition in greenhouse controls as temperature 28 DEG C ~ 30 DEG C, and humidity is 70%, natural lighting, choose the banana seedlings that growing way is consistent, nutrition pot fills native 500g/ alms bowl, banana seedlings is planted into wherein.Test establishes 3 process, 1) space management: use clear water; 2) fermentation liquor treatment: pathogenic bacteria+use fermented liquid stoste; 3) CK: pathogenic bacteria+use clear water.3 repetitions are established in each process, each repetition 30 strain banana seedlings.Pathogenic bacteria treatment process is: by FOC4 activation culture 5d on PDA flat board, adopts the method for aseptic water washing to wash out pathogenic bacteria spore, makes spore suspension, microscopy be 10 by spore suspension dilution
5cFU/mL concentration.Before transplanting, adopt root-pouring method inoculation pathogenic bacteria, inoculum density is 10
5the spore suspension of CFU/mL.Each treatment group is in transplanting after 1 day, and fermentation liquor treatment group applies the fermented liquid stoste of dilution 50 times, and amount of application is 200mL/ alms bowl; CK group and space management group apply equivalent clear water; Every 5 days, repetitive administration once, amounted to 9 times again.Duration of test, each other control measures of process are consistent.When 60d transplanted by banana, record each process every strain banana yellow blade and the normal number of blade and calculate disease index grade, it the results are shown in Table 6.
Banana disease index and protection effect calculate according to following formula:
Disease index=Σ (the plant number of rank × this rank of disease)/confession examination plant
Severity Scaling: 0 grade is asymptomatic; 1 grade is only had foot 1 yellow leaf slightly to wilt for whole strain blade; 2 grades is that the flavescence of 1-2 sheet blade is wilted; 3 grades is that the flavescence of complete stool 1/3-1/2 blade is wilted; 4 grades is that the flavescence of complete stool 1/2-3/4 blade is wilted; 5 grades is that complete stool 3/4 is wilted or whole strain death with blade flavescence.
Table 6 bacterial strain fermentation liquor is to the prevention effect of potted plant banana seedlings blight
As can be seen from Table 6, use the treatment group of fermented liquid stoste+pathogenic bacteria, disease index obviously reduces than the treatment group using pathogenic bacteria+clear water, and protection effect can reach more than 69.82%.Illustrate that Lu Nalinrui streptomycete bacterial strain fermented liquid of the present invention has significant preventive and therapeutic effect to potted plant banana seedlings blight.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.
Claims (10)
1. Yi Zhong Lu Nalinrui streptomycete, it is characterized in that, called after Lu Nalinrui streptomycete (Streptomyces lunalinharesii) B-03, China typical culture collection center is preserved on March 23rd, 2015, address is in the Wuhan University of Wuhan City, Hubei Province, and deposit number is CCTCC NO:M2015148.
2. a kind of Lu Nalinrui streptomycete according to claim 1, is characterized in that: the 16S rRNA gene order of described Lu Nalinrui streptomycete B-03 is as shown in SEQ ID NO.1.
3. the application of the Lu Nalinrui streptomycete B-03 described in preparation control banana blight bacteria preparation.
4. the Lu Nalinrui streptomycete B-03 described in is in the application of the preparation of preparation suppression musae, the microscler leaf spot fungi of banana, the large Cercospora Sojina Hara of banana, tribute any of several broadleaf plants eye spot bacterium, banana leaf margin wilt, lichee anthrax bacteria.
5. Yi Zhong Lu Nalinrui streptomycete bacterial strain fermented liquid, it is characterized in that: Lu Nalinrui streptomycete B-03 is inoculated in after soyflour fermention medium 28 DEG C cultivates 3 ~ 5d and obtains fermentation seed liquid, described fermentation seed liquid is inoculated in soya-bean cake substratum, 28 DEG C ~ 30 DEG C, 180rpm shakes cultivation 7 ~ 10d, namely obtains described bacterial strain fermentation liquor.
6. Lu Nalinrui streptomycete bacterial strain fermented liquid according to claim 5, is characterized in that: described soyflour cultivate and composition, be designated as by weight: soyflour 5 ~ 10 parts, NaCl 1 ~ 5 part, CaCO
31 ~ part, peptone 2 ~ 5 parts, glucose 5 ~ 10 parts; Described soyflour Medium's PH Value is 7.0 ~ 7.5; The composition of described soya-bean cake fermention medium, is designated as by weight: 180 ~ 200 parts, brown sugar, soya-bean cake 50 ~ 70 parts, 800 ~ 1200 parts, water; Fermentation seed liquid is got according to 10% of soya-bean cake fermention medium volume.
7. a kind of Lu Nalinrui streptomycete bacterial strain fermented liquid according to claim 5 or 6 suppresses the application of Fusarium oxysporum No. 1 microspecies, Fusarium oxysporum No. 4 microspecies and colletotrichum gloeosporioides Penz preparation in preparation.
8. a kind of Lu Nalinrui streptomycete bacterial strain fermented liquid according to claim 5 or 6 suppresses the application of banana blight preparation in preparation.
9. the application of a kind of Lu Nalinrui streptomycete bacterial strain fermented liquid according to claim 7 or 8, is characterized in that, after described bacterial strain fermentation liquor dilution, is directly applied to field crop.
10. the application of a kind of Lu Nalinrui streptomycete bacterial strain fermented liquid according to claim 9, described bacterial strain fermentation liquor application process is, bacterial strain fermentation liquor dilution 50 ~ 100 times is filled with root afterwards or carried out ground and sprays.
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| CN107354103A (en) * | 2017-04-21 | 2017-11-17 | 浙江师范大学 | Streptomycete Streptomyces lunalinharesii ZJNU968 bacterial strains and its application |
| CN107904196A (en) * | 2017-12-26 | 2018-04-13 | 中国热带农业科学院热带生物技术研究所 | A kind of Yan Shi streptomycetes and its application |
| CN108048380A (en) * | 2018-02-11 | 2018-05-18 | 海南大学 | One plant of Deccan streptomycete QY-3 and its application |
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| CN107354103A (en) * | 2017-04-21 | 2017-11-17 | 浙江师范大学 | Streptomycete Streptomyces lunalinharesii ZJNU968 bacterial strains and its application |
| CN107354103B (en) * | 2017-04-21 | 2020-05-26 | 浙江师范大学 | Streptomyces lunalinharesii ZJNU968 strain and application thereof |
| CN107043726A (en) * | 2017-05-26 | 2017-08-15 | 福建省农业科学院植物保护研究所 | One plant of actinomyces SVFJ 07 and its application in preventing and treating cymbidium anthracnose |
| CN107043726B (en) * | 2017-05-26 | 2019-08-30 | 福建省农业科学院植物保护研究所 | One plant of actinomyces SVFJ-07 and its application in prevention and treatment cymbidium anthracnose |
| CN107904196A (en) * | 2017-12-26 | 2018-04-13 | 中国热带农业科学院热带生物技术研究所 | A kind of Yan Shi streptomycetes and its application |
| CN107904196B (en) * | 2017-12-26 | 2020-04-07 | 中国热带农业科学院热带生物技术研究所 | Streptomyces yanshi and application thereof |
| CN108048380A (en) * | 2018-02-11 | 2018-05-18 | 海南大学 | One plant of Deccan streptomycete QY-3 and its application |
| CN110656116A (en) * | 2019-10-17 | 2020-01-07 | 华南农业大学 | Application of gene FoCWM in regulation and control of pathogenicity of banana vascular wilt |
| CN110628687A (en) * | 2019-10-24 | 2019-12-31 | 广西科学院 | Streptomyces 5017 and application thereof in antagonism of phytopathogens |
| CN113583892A (en) * | 2021-06-08 | 2021-11-02 | 中国热带农业科学院热带生物技术研究所 | Streptomyces corviensis and application thereof |
| CN115627248A (en) * | 2022-12-21 | 2023-01-20 | 中国热带农业科学院三亚研究院 | Broad-spectrum antifungal streptomyces neoformans, microbial inoculum thereof, preparation method and application |
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