CN108913621A - One plant of Methylotrophic bacillus YH-18 for effectively preventing oriental cherry root knot and its application - Google Patents
One plant of Methylotrophic bacillus YH-18 for effectively preventing oriental cherry root knot and its application Download PDFInfo
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Abstract
The Methylotrophic bacillus YH-18 for effectively preventing oriental cherry root knot the invention discloses one plant and its application; the Methylotrophic bacillus classification naming is Methylotrophic bacillus (Bacillus methylotrophicus) YH-18; it has been preserved in China typical culture collection center, deposit number CCTCC NO：M 2018006, preservation day are on January 4th, 2018, preservation address：Wuhan, China Wuhan University.Methylotrophic bacillus YH-18 provided by the invention is plant endogenesis biological and ecological methods to prevent plant disease, pests, and erosion growth-promoting bacterium, has preferable control efficiency to oriental cherry root knot, while having growth-promoting functions to the growth of oriental cherry.Methylotrophic bacillus YH-18 condition of culture is simple, is easy to save, easy to industrialized production, with good development and application prospects.
Description
Technical field
The invention belongs to microorganisms technical fields, and in particular to one plant of Methylotrophic bud for effectively preventing oriental cherry root knot
Spore bacillus (Bacillus methylotrophicus) YH-18 and its application.
Background technique
Oriental cherry is world-renowned ornamencal flower and tree, is under the jurisdiction of rosaceae (Rosaceae) cherry category (Cerasus), is distributed in north
The warm area of hemisphere is north temperate zone plant.Oriental cherry system shallow root plant likes slightly sour loose fertile, well-drained soil and back
The wet environment of wind direction sun.China possesses the oriental cherry resource of very abundant, non-considerably beyond Japan and neighboring countries, distribution
Often extensively, it is all distributed from northeast to southwest, the high type of many of them ornamental value.In recent years oriental cherry because its plant type it is excellent
Beauty, pattern is gorgeous, florescence duration is long and the extensive attention for the person that causes cultivating seedlings with high ornamental value, but with
Being continuously increased for cultivated area, the area of suffered disease infestation also constantly expand, wherein with oriental cherry root knot, oriental cherry shothole disease
And oriental cherry bleeding disease is the most serious.
Root knot is also referred to as root knot, root head cancer, clubroot, it is that as caused by Agrobacterium tumefaciems and have serious harm
Bacterial disease, the disease have different degrees of generation in worldwide.Song Yu is bis- et al. the study found that soil Agrobacterium
Host range it is very extensive, 93 sections 331 can be infected and belong to more than 643 different plants, wherein to endanger dicotyledon
Based on, gymnosperm and monocotyledon can also be infected under specific condition.The infection way of root knot mainly passes through wound.
Site of pathological change is mainly rootstock, lateral root and limb, and classical symptom is to form the different tumour of shape size in position of disease.Morbidity
The symptom at position initial stage is to be initially formed milky, knob small such as beans, later with the growth knob of nursery stock by
It gradually grows up, color is gradually deep, and surface becomes coarse by smooth, and then the quality become by internal lignifying is hard, shows dark brown
Color becomes irregular shape after withered cracking, and knurl can surround rootstock or branch when which occurs serious.Illness is early
The aerial part non-evident sympton of phase, nursery stock or tree body.With becoming larger for tumour, plant imaginary root tails off, tree vigo(u)r by dying down by force, into
And yellowing leaf is caused, untimely defoliation.When falling ill serious, plant will receive influence in terms of nutrient absorption and Water Transportation,
Plant strain growth slows down, and yield declines year by year, last until dead.
Oriental cherry root knot is caused by Agrobacterium tumefaciens, and very big loss is caused in production, is put within 1996 years
Domestic forest quarantine object list.It is that spherical or hemispherical knob is generated at rhizome that oriental cherry, which obtains most apparent symptom after being ill,
Knob is slowly grown up with the growth of nursery stock, has been seriously affected oriental cherry seedling growth speed and tree vigo(u)r, has been reduced ornamental article
Matter and commodity value.Disease causes to fall leaves in advance when occurring light, can cause whole tree withered when serious, be known as oriental cherry by flower-grower
" cancer ".
The current means of prevention for oriental cherry root knot is mainly based on chemical prevention and biological control.Utkhede RS etc.
(1993) root for handling morbidity treelet with Kocide SD solution in apple field trial, as a result, it has been found that test group nodule number
It is reduced compared with control group；Suppression of the Wang Huimin etc. (1995) by measurement fungicide and antibiotic to 3 kinds of bion Agrobacterium tumefaciems
Bacterium effect, research find that streptomycin sulphate is all fine to the inhibiting effect of whole crown gall bacterium；Thiram is to biological type III crown gall agriculture
All bacterial strains of bacillus have to be acted on compared with high inhibition；Benzylpenicillin sodium salt only has good inhibiting effect to biological type III；Other medicaments are equal
Without effect.But it is long-term can bring a series of serious problems to environment and the ecological balance using chemical pesticide, and biological control
In recent years by favor due to its is at low cost, pollution is small, control efficiency is significant, some scientific workers prevent and treat work in root knot
Also variety classes and the significant biocontrol bacterial strain of control efficiency have been filtered out during work.Kerr is taught from peach root knot illness plant
Isolated bacterial strain K84 in rhizosphere soil can effectively inhibit pathological form Agrobacterium tumefaciens to grow；Akira (2007) from
Isolated grape agrobacterium VAR03-1, no pathogenicity on the grape nursery stock of Japan, and a kind of bacteriocin can be generated, it can
To inhibit the formation of the tumour as caused by pathogenic grape agrobacterium G-Ag-27 on tomato and grape.
Bacillus has become the dominant strain of biocontrol microorganisms because cultivation cycle is short, convenient for production.It is a kind of production bud
The gram-positive bacterium of spore, aerobic or amphimicrobian life, can generate heat-resisting, drought-enduring, uvioresistant and resist organic molten
The endospore of agent, produced gemma can be made into the various dosage forms such as pulvis, wettable powder, and it is mixed with chemical pesticide after do not lose
It is living, it is ideal screening of biocontrol agents object.
Clover is the model plant for being generally used for hospital's Bacteria infecting respiratory Pathogenicity.Laura in 2002 is with one
Kind is inoculated with clover and mouse in the pathogen Psudomnoas aeruginosa that CF is often detected in patient body respectively, compares
Result of study discovery, clover can replace the toxicity or even clover mould that mouse measures the bacterium to mammal as model plant
Type shows sensitivity more higher than mouse models (Laura et al., 2002).Then, Steve has done similarly Bcc
Compare test, it is found that disease incidence of the Bcc on clover is positively correlated with the result on mouse, can replace mouse as measurement
Bcc is to the model plant of animal toxicity (Steve et al., 2003).With clover model can be simple and fast detect Bcc bacterium
It is pathogenic whether strain has mammal, this has established solid foundation for our the safe utilization Bc in agricultural and environmental protection.
In recent years, the research in relation to bacillus in oriental cherry root knot field of biological control is rarely reported.Therefore, by bud
Biological control of the spore bacillus for oriental cherry root knot works, and is conducive to preferably prevent and treat the disease.
Summary of the invention
Goal of the invention：For the deficiencies in the prior art, the object of the present invention is to provide one kind to effectively prevent oriental cherry
The Methylotrophic bacillus YH-18 of root knot.It is a further object of the present invention to provide above-mentioned Methylotrophic bacillus
Purposes.
Technical solution：In order to solve the above technical problems, the technical solution adopted by the present invention is as follows：
One plant of bacterium for effectively preventing oriental cherry root knot, classification naming are Methylotrophic bacillus (Bacillus
Methylotrophicus) YH-18 has been preserved in China typical culture collection center, deposit number CCTCC NO：M
2018006, preservation day is on January 4th, 2018, preservation address：Wuhan, China Wuhan University.
This effectively prevents the Methylotrophic bacillus (Bacillus methylotrophicus) of oriental cherry root knot
YH-18 is one plant of biocontrol bacteria bacterium to crown gall germ with antagonism that separation screening obtains from healthy oriental cherry limb
Strain.Methylotrophic bacillus (Bacillus methylotrophicus) YH-18 is in beef-protein medium plate
Upper bacterium colony is smaller, and white, the general 2-3mm of diameter, round, edge is irregular, and rough surface has lamella, thallus direct rod shape, size
For 0.7 × (1~2) μm.Gram's staining shows as the positive；16SrDNA major part sequence 1418bp is measured, it is specific such as SEQ ID
Shown in NO.1.Surveyed 16SrDNA sequence is compared by BLAST, the very high close bacterium of homology can be found in GenBank
Strain sequence.Highest with YH-18 bacterial strain similitude is Bacillus methylotrophicus, and homology all reaches 100%.
Chadogram discovery is constructed, YH-18 and Bacillus methylotrophicus belong to a hereditary branch, and affiliation is very
It is close.Find the bacterium indole reaction for the positive by physiological and biochemical test；Catalase reaction result is the positive；Clark and Lubsreaction knot
Fruit is the positive；Starch Hydrolysis test is the positive；V-P test, nitrate reduction reaction and citrate reaction result are also all presented
It is positive；Therefore, it is determined that the bacterium is Methylotrophic bacillus (Bacillus methylotrophicus), classification naming is first
Base auxotype bacillus (Bacillus methylotrophicus) YH-18.
Application of the Methylotrophic bacillus YH-18 in prevention and treatment oriental cherry root knot.
Application of the metabolite of the Methylotrophic bacillus YH-18 in prevention and treatment oriental cherry root knot.
The application, the metabolite are the fermentation liquid of Methylotrophic bacillus YH-18.
The Methylotrophic bacillus YH-18 is inhibiting the application in Agrobacterium tumefaciems.
The metabolite of the Methylotrophic bacillus YH-18 is inhibiting the application in Agrobacterium tumefaciems.
The application, the metabolite are the fermentation liquid of Methylotrophic bacillus YH-18.
The Methylotrophic bacillus YH-18 is promoting the application in oriental cherry growth.
The metabolite of the Methylotrophic bacillus YH-18 is promoting the application in oriental cherry growth.
The application, the metabolite are the fermentation liquid of Methylotrophic bacillus YH-18.
The preferable fermentation condition of above-mentioned Methylotrophic bacillus YH-18 control efficiency is as follows：
Culture medium：Every 1000mL distilled water, 3g containing beef extract, peptone 10g, sodium chloride 5g, pH7.2.
Process conditions are：PH6~7,1: 1,28 DEG C~30 DEG C of ventilatory capacity, 180~200 revs/min of shaking speed, 24 hours.
It is a kind of microbial bactericide by the fermentation liquid that above-mentioned zymotechnique obtains.The fermentation liquid and water are according to 1: 5
Dilution proportion is simultaneously added appropriate soil and breaks into mud and dip in slurry processing to plant naked and can play its bactericidal effect.
Beneficial effect：Compared with prior art, Methylotrophic bacillus YH-18 provided by the invention is plant roots
Biological and ecological methods to prevent plant disease, pests, and erosion growth-promoting bacterium in border has preferable control efficiency to oriental cherry root knot, while having growth-promoting functions to the growth of oriental cherry.The first
Base auxotype bacillus YH-18 condition of culture is simple, is easy to save, easy to industrialized production, has good development and application
Prospect.
Detailed description of the invention
Fig. 1 is the antibacterial result figure of Methylotrophic bacillus YH-18；
Fig. 2 is antagonism result figure of the ferment filtrate to Agrobacterium tumefaciens of Methylotrophic bacillus YH-18；
Fig. 3 is resistant effect result figure of the Methylotrophic bacillus YH-18 to greenhouse indicator plant root knot；
Fig. 4 is resistant effect result figure of the Methylotrophic bacillus YH-18 to greenhouse oriental cherry root knot；
Fig. 5 is Methylotrophic bacillus YH-18 to clover seedling inoculation experiments result figure.
Specific embodiment
The present invention is described further combined with specific embodiments below.It, can be more preferable geographical according to following embodiments
The solution present invention.However, as it will be easily appreciated by one skilled in the art that specific material proportion, process conditions described in embodiment
And its result is merely to illustrate the present invention, without the present invention described in detail in claims should will not be limited.
Embodiment 1：Methylotrophic bacillus YH-18 bacterial strain measures them to crown gall germ bacterium by agar diffusion method
The inhibiting effect of strain growth.
The Agrobacterium tumefaciems cfcc1369 (buying from China Forest Microbiological Culture Collection administrative center) that purifying is cultivated,
Training, which is shaken, with LB culture medium is made 107~108Agrobacterium tumefaciems cfcc-1369 bacteria suspension 200uL is coated on by cfu/mL bacteria suspension
Filter paper is fabricated to the disk of diameter 0.6mm using punch by LB solid medium, 3 pieces of filter paper points is connected to coated
On plate.Training is shaken to 10 with LB culture medium7~108The Methylotrophic bacillus YH-18 bacteria suspension 10uL point of cfu/mL is being filtered
On the scraps of paper, culture dish is sealed and places overnight incubation in 28 DEG C of incubators.The result shows that as shown in Figure 1, the midpoint Fig. 1 A, B, C are
The inhibition zone that Methylotrophic bacillus YH-18 forms Agrobacterium tumefaciems cfcc-1369, the midpoint Fig. 1 D are CK control, can
See that the fungistatic effect of Methylotrophic bacillus YH-18 is preferable.
Embodiment 2：Methylotrophic bacillus YH-18 bacterial strain fermentation liquor and root knot bacteria strain carry out the antibacterial energy of plate
The measurement of power.
The glycerol stocks liquid of Methylotrophic bacillus YH-18 bacterial strain is inoculated into beef extract egg by 2% inoculum concentration
In white peptone fluid nutrient medium, training is shaken for 24 hours.Ferment product is filtered with 0.22 μm of biofilter and is filtered up to bacterial fermentation
Liquid.Training will be shaken to 107~108The Agrobacterium tumefaciems cfcc-1369 bacteria suspension 200uL coating of cfu/mL on LB plate, using beating
Filter paper is fabricated to the disk of diameter 0.6mm by hole device, and 3 pieces of filter paper points are connected on coated plate.10uL methyl is sought
It supports type bacillus YH-18 ferment filtrate point to be connected on filter paper, culture dish sealing is placed in overnight incubation in 28 DEG C of incubators.
The result shows that as shown in Fig. 2, left figure is Methylotrophic bacillus YH-18 ferment filtrate to Agrobacterium tumefaciems cfcc-1369
Good inhibition zone is formed, right figure is control, it is seen that Methylotrophic bacillus YH-18 ferment filtrate is antibacterial preferably.
Embodiment 3：Resistant effect of the Methylotrophic Bacillus strain YH-18 to greenhouse indicator plant root knot
Tomato seedling is bought, places it in mixed cultivating soil and cultivates.Inoculation (choosing when seedling stretches out soil face 15cm or so
Take the similar healthy and strong seedling of growing way), half an hour takes out bacterium Methylotrophic bacillus YH-18 waiting before inoculation, makes to cultivate
The temperature of bacterium is restored to room temperature, then starts to be inoculated with.First by dissecting needle on alcolhol burner incineration, be cooled to room to dissecting needle
Wen Hou, the bacterium after picking isolates and purifies pierce to the stem of seedling, dip sterile water with absorbent cotton wool and use sealed membrane at inoculation
Bondage, 5~7d of moisturizing；Half an hour takes out bacterium waiting before being inoculated with oriental cherry Agrobacterium tumefaciems cfcc-1369, keeps the temperature for cultivating bacterium extensive
Again to room temperature, then start to be inoculated with.First by dissecting needle on alcolhol burner incineration, after dissecting needle is cooled to room temperature, picking
Bacterium after isolating and purifying pierces to seedling rhizome, dips sterile water at inoculation with sealed membrane bondage, moisturizing 5 with absorbent cotton wool
~7d.Inoculating strain YH-18 and pathogen cfcc-1369；Individually inoculation cause of disease bacteria strain is used as control, does not connect bacterium as blank
Control, 5 repetitions are done in totally three processing, each processing, are that growth is cultivated in the greenhouse of 5 DEG C of 23 scholar in room temperature.Observing after 1 week is
No morbidity.As a result, it has been found that being only inoculated with the obvious enlargement of base of the plant of peach root knot germ, while inoculating strain YH-18 and pathogen
The slightly enlargement of the base of the plant of bacterial strain, show YH-18 have certain resistant effect (Fig. 3, a left side be and meanwhile be inoculated with YH-18 and cause of disease
Bacterium, the right side are only to be inoculated with pathogen).
Embodiment 4：Control efficiency of the Methylotrophic bacillus YH-18 to greenhouse oriental cherry seedling root knot.
By the plantation of annual oriental cherry in sterilized soil, 25 DEG C of hot-house culture 15d are placed in, oriental cherry similar in growing way is chosen and carries out
It is inoculated with (18 repetitions of each processing).Every plant of oriental cherry inoculation crown gall pathogen cfcc-1369 (NA fluid nutrient medium, culture is for 24 hours)
2mL, the Methylotrophic bacillus YH-18 that inoculation inclined-plane saves are seeded to the culture of NA culture medium to 107~108Cfu/mL,
Methylotrophic bacillus is diluted to concentration 1 × 10 with sterile water8Cfu, it is spare to be made bacterium suspension, with aseptic operation knife
1cm wound is drawn in oriental cherry main root, Agrobacterium tumefaciens are drawn with sterile cotton balls and are wrapped at wound；It is inhaled after 7d with Sterile pipette
It takes 10mL Methylotrophic bacillus suspension to be close to oriental cherry seedling root system and is injected into soil, then earthing is filled and led up to plastics
Basin.Oriental cherry seedling singly to connect Agrobacterium tumefaciens is compareed as morbidity, and the disease incidence and disease tumor of oriental cherry are observed and counted after 6 months
Diameter.Methylotrophic bacillus YH-18 has significant effect (table 1, Fig. 4) in the prevention and treatment of greenhouse oriental cherry seedling root knot.Fig. 4
Upper figure is the morbidity control for being inoculated with root knot bacillus, and the following figure is the Prevention technology for being inoculated with Agrobacterium tumefaciems and YH-18.
Control efficiency of 1 JK-XZ3 of table to greenhouse oriental cherry seedling root knot
Embodiment 5：The disease resisting effect of the Methylotrophic field Bacillus strain YH-18 prevention and treatment oriental cherry root knot
Methylotrophic Bacillus strain YH-18 is applied to the field field plots that root knot seriously occurs, takes mother
Bacterium solution 2L adds in 10L barrels of water constant volume, dilutes 5 times, stirs and evenly mixs, and adds appropriate soil stirring in thick, is made into slurries.It will be naked
Root oriental cherry seedling is put into bucket one by one submerges root, takes out after one layer of mud is wrapped up on surface, is put into the soil pit plant dug in advance.
Test result shows that the bacterial strain has good prevention and treatment root knot effect to annual new afforestation health oriental cherry.It is stained with slurry processes in root
100 oriental cherry nursery stocks for applying the bacterial strain, the results are shown in Table 2.Therefore, it is determined that the bacterial strain can effectively prevent oriental cherry root knot.Except this
Except YH-18 bacterial strain also there is excellent growth-promoting functions (table 3) to annual oriental cherry seedling.
The annual oriental cherry of table 2 tests seedling morbidity statistics
The annual oriental cherry seedling increment of table 3 statistics
| Processing | Height of seedling (cm) | Ground diameter (mm) |
| CK | 12.1 | 3.4 |
| YH-18 | 34.2 | 6.8 |
Embodiment 5：Methylotrophic Bacillus strain YH-18 is to onion Pathogenicity.
Pathogenicity takes Methylotrophic Bacillus strain YH- with syringe referring to the method for (2005) such as Sijam
18 bacteria suspensions (1 × 107~108Cfu/mL) two point symmetries injection (depth is 1~2cm) on onion bulb stem, every injection volume is
1mL.With the water process that sterilizes for control.All processing are repeated 3 times, and are placed in 26 DEG C of cultures, inspection result after 4~5 days.Onion squama
Stem inoculation test shows that injection inoculation Methylotrophic Bacillus strain YH-18 does not cause rotting for onion bulb stem, only exists
Slightly shrivelled recess at inoculation.Methylotrophic Bacillus strain YH-18 described above is not plant pathogenetic bacteria, to plant
With safety.
Embodiment 6：Inoculation test of the Methylotrophic Bacillus strain YH-18 to clover.
YH-18 is inoculated into NB culture medium, (150mrp) culture is shaken at 30 DEG C for 24 hours, adjusts bacteria suspension with sterile water
Concentration is to 109Left and right is for being inoculated with.Prepare the alfalfa seed to be inoculated with (Laura et al., 2002) with the method for Laura.
98% concentrated sulfuric acid of alfalfa seed is impregnated 20 minutes (about 10mL soaks 300 seeds), is cleaned 4 times with 500mL aqua sterilisa,
Then it steeps in 60mL aqua sterilisa, (150prm) is shaken at 32 DEG C and is soaked seed 6~8 hours, then is cleaned 2 times, similarity condition after water is changed
Overnight incubation.After next day cleans 2 times with aqua sterilisa again (each 60mL water), the water agar plate to 1% is put with aseptic nipper
First-class to be seeded, every ware puts 20 seeds.Alfalfa seed transfer needle on water agar plate is pierced on cotyledon one small
Hole takes the ready bacteria suspension point of 20mL in wound, 3 repetitions of every strain inoculated, 20 seeds of every repetition, if at 2 kinds of CK
Reason, wherein 1 with no treatment, another 1 stab inoculation sterile water.Seed after inoculation 37 DEG C, it is 70% relative humidity, fixed
It is cultivated in the growth cabinet of Shi Guangzhao (12h illumination, 12h are dark) condition, checks disease incidence after 7 days.
Clover inoculation is the results show that either CK or inoculation processing, and without disease symptom, (vaccination is downright bad nearby, yellow
Change and root be abnormal) occur.Clover be instead of mouse as measure Bcc to the model plant of animal toxicity (Steve et al.,
2003), with clover model can be simple and fast to detect whether Bcc bacterial strain has mammal pathogenic.Above-mentioned test knot
Fruit illustrates that Methylotrophic Bacillus strain YH-18 does not have toxic to mammal.Left in Fig. 5 is Methylotrophic gemma
Bacillus YH-18 inoculation, in be pyrroles's Berghold Salmonella pathological form LMG1222, the right side be clear water compare.
Sequence table
<110>Nanjing Forestry University
<120>One plant of Methylotrophic bacillus YH-18 for effectively preventing oriental cherry root knot and its application
<130> 100
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1387
<212> DNA
<213> Bacillus methylotrophicus
<400> 1
acatgcaagt cgagcgacag atgggagctt gctgcctgat gttagcggcg gacgggtgag 60
taacacgtgg gtaacctgcc tgtaagactg ggataactcc gggaaccggg gctaataccg 120
gatggttgtt tgaaccgcat ggttcagaca taaaggtggc ttcggctacc acttacagat 180
ggacccgcgg cgcattagct agttggtgag gtaacggctc accaaggcaa cgatgcgtag 240
ccgacctgag agggtgatcg gccacactgg actgagacac gggccagact cctacgggag 300
gcagcagtag ggaatcttcc gcaatggacg aaagtctgac ggagcaacgg cgcgtgagtg 360
atgaaggttt tcggatcgta aagctctgtt gttaggaaga acaagtgccg ttcaaatagg 420
gcggcacctt gacggtacct accagaaagc cacggctaac tacgtgccag caggcgcggt 480
aatacgtagg tggcaagcgt tgtccggaat tattgggcgt aaagggctcg caggcggttt 540
cttaagtctg atgtgaaagc cgccggctca accgggaggt cattggaact ggggaacttg 600
agtgcagaag aggagagtgg aattccacgt gtagcggtga atgcgtagag atgtggagga 660
acaccagtgg cgaaggcgac ttctctggtc tgtaactgac gctgaggagc gaaagcgtgg 720
gagcgaacag gattagatac cctggtagtc cacgccgtaa acgatgagtg ctaagtgtta 780
ggtcgcccct tagtgctgca gctaacgcat taagcactcc gcctgggagt acggtcgcaa 840
gactgaaact caaaggaatt gacgggcccg cacaagcggt ggagcatgtg gtttaattcg 900
aagcaacccg aagaacctta gcaggtcttg acatcctctg acaatcctag agataggacg 960
tcgccttcgg gggcagagtg acaggtggtg catggttgtc gtcagctcgt gtcgtgagat 1020
gttgggtaag tcccgcaacg agccaacctt gatcttagtt gccagcattc agttggcact 1080
ctaaggtgac tgccggtgac aagcgaggaa ggtggggatg acgtcaaatc atcatgcccc 1140
ttatgacctg gctacacacg tgctacaatg gacagaacaa agggcagcga aaccgcgagg 1200
ttaagccaat cgcacaaatc tgttctcagt tcggatcgca gtctgcaact cgctcgtgaa 1260
gctggaatcg ctagtaatcg cggatcagca tgccgcggtg aatacgttcc cggccttgta 1320
cacaccgccc gtcacaccac gagagtgtaa cacccgaagt cggtgaggta accttttagg 1380
agccagc 1387
Claims (10)
1. one plant of bacterium for effectively preventing oriental cherry root knot, classification naming is Methylotrophic bacillus (Bacillus
Methylotrophicus) YH-18 has been preserved in China typical culture collection center, deposit number CCTCC NO：M
2018006, preservation day is on January 4th, 2018, preservation address：Wuhan, China Wuhan University.
2. application of the Methylotrophic bacillus YH-18 described in claim 1 in prevention and treatment oriental cherry root knot.
3. the metabolite of Methylotrophic bacillus YH-18 described in claim 1 answering in prevention and treatment oriental cherry root knot
With.
4. application according to claim 3, the metabolite is the fermentation of Methylotrophic bacillus YH-18
Liquid.
5. Methylotrophic bacillus YH-18 described in claim 1 is inhibiting the application in Agrobacterium tumefaciems.
6. the metabolite of Methylotrophic bacillus YH-18 described in claim 1 is inhibiting answering in Agrobacterium tumefaciems
With.
7. application according to claim 6, the metabolite is the fermentation of Methylotrophic bacillus YH-18
Liquid.
8. Methylotrophic bacillus YH-18 described in claim 1 is promoting the application in oriental cherry growth.
9. the metabolite of Methylotrophic bacillus YH-18 described in claim 1 is promoting the application in oriental cherry growth.
10. application according to claim 9, the metabolite is the fermentation of Methylotrophic bacillus YH-18
Liquid.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110079470A (en) * | 2019-03-04 | 2019-08-02 | 宁波城市职业技术学院 | One plant of pseudomonad with bacteriostatic activity |
| CN110627566A (en) * | 2019-09-29 | 2019-12-31 | 云南云之叶生物科技有限公司 | Bio-organic fertilizer with clubroot disease prevention and treatment function and preparation method and application thereof |
| CN113774001A (en) * | 2021-11-02 | 2021-12-10 | 盐城师范学院 | High-yield C21Steroid glycoside agrobacterium tumefaciens F-45 and application thereof |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106686983A (en) * | 2014-07-31 | 2017-05-17 | 格拉纳达大学 | Use of bacillus methylotrophicus as a stimulant of plant growth and biological control means, and isolates of said species |
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2018
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106686983A (en) * | 2014-07-31 | 2017-05-17 | 格拉纳达大学 | Use of bacillus methylotrophicus as a stimulant of plant growth and biological control means, and isolates of said species |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110079470A (en) * | 2019-03-04 | 2019-08-02 | 宁波城市职业技术学院 | One plant of pseudomonad with bacteriostatic activity |
| CN110627566A (en) * | 2019-09-29 | 2019-12-31 | 云南云之叶生物科技有限公司 | Bio-organic fertilizer with clubroot disease prevention and treatment function and preparation method and application thereof |
| CN110627566B (en) * | 2019-09-29 | 2021-11-16 | 云南云之叶生物科技有限公司 | Bio-organic fertilizer with clubroot disease prevention and treatment function and preparation method and application thereof |
| CN113774001A (en) * | 2021-11-02 | 2021-12-10 | 盐城师范学院 | High-yield C21Steroid glycoside agrobacterium tumefaciens F-45 and application thereof |
| CN113774001B (en) * | 2021-11-02 | 2023-03-14 | 盐城师范学院 | High-yield C 21 Steroid glycoside agrobacterium tumefaciens F-45 and application thereof |
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| CN108913621B (en) | 2020-05-12 |
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