CN114027093A - Method for cultivating ganoderma lucidum spore powder with high content of triterpene - Google Patents
Method for cultivating ganoderma lucidum spore powder with high content of triterpene Download PDFInfo
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- CN114027093A CN114027093A CN202111620857.XA CN202111620857A CN114027093A CN 114027093 A CN114027093 A CN 114027093A CN 202111620857 A CN202111620857 A CN 202111620857A CN 114027093 A CN114027093 A CN 114027093A
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- ganoderma
- spore powder
- ganoderma lucidum
- triterpene
- fruiting
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- 239000000843 powder Substances 0.000 title claims abstract description 54
- 240000008397 Ganoderma lucidum Species 0.000 title claims abstract description 43
- 235000001637 Ganoderma lucidum Nutrition 0.000 title claims abstract description 43
- 150000003648 triterpenes Chemical class 0.000 title claims abstract description 25
- 238000000034 method Methods 0.000 title claims abstract description 18
- 241000233866 Fungi Species 0.000 claims abstract description 27
- 239000002689 soil Substances 0.000 claims abstract description 11
- 241000222336 Ganoderma Species 0.000 claims description 40
- 238000003306 harvesting Methods 0.000 claims description 8
- 239000004745 nonwoven fabric Substances 0.000 claims description 8
- 230000001954 sterilising effect Effects 0.000 claims description 8
- 239000000463 material Substances 0.000 claims description 5
- 238000001816 cooling Methods 0.000 claims description 4
- 230000007423 decrease Effects 0.000 claims description 4
- 238000011081 inoculation Methods 0.000 claims description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims description 4
- 238000009423 ventilation Methods 0.000 claims description 4
- 150000004676 glycans Chemical class 0.000 abstract description 7
- 229920001282 polysaccharide Polymers 0.000 abstract description 7
- 239000005017 polysaccharide Substances 0.000 abstract description 7
- 230000000052 comparative effect Effects 0.000 description 3
- 238000012364 cultivation method Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 2
- XBZYWSMVVKYHQN-MYPRUECHSA-N (4as,6as,6br,8ar,9r,10s,12ar,12br,14bs)-10-hydroxy-2,2,6a,6b,9,12a-hexamethyl-9-[(sulfooxy)methyl]-1,2,3,4,4a,5,6,6a,6b,7,8,8a,9,10,11,12,12a,12b,13,14b-icosahydropicene-4a-carboxylic acid Chemical compound C1C[C@H](O)[C@@](C)(COS(O)(=O)=O)[C@@H]2CC[C@@]3(C)[C@]4(C)CC[C@@]5(C(O)=O)CCC(C)(C)C[C@H]5C4=CC[C@@H]3[C@]21C XBZYWSMVVKYHQN-MYPRUECHSA-N 0.000 description 1
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 1
- 241000218645 Cedrus Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003864 humus Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- -1 varieties Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 239000002023 wood Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention provides a method for cultivating ganoderma lucidum spore powder with high content of triterpene, which has the technical core that the environment of fruiting areas is selected as follows: 31-33 degrees north latitude, elevation 900-; planting requirements are as follows: under the fruiting field, furrows with the depth of 18-22cm and the width of 35-40cm are formed along the inter-tree edges, the fungus sticks with full hyphae are transported to the fruiting field, the fungus sticks are transversely placed, the distance between the fungus sticks is 8-12cm, then soil is covered, and the ganoderma lucidum growth management process is carried out. According to the invention, the specific latitude range and altitude range are selected as the growing environment of the ganoderma lucidum, and the produced ganoderma lucidum spore powder can obviously improve the content of effective medicinal components such as polysaccharide, triterpene and the like.
Description
Technical Field
The invention relates to the technical field of ganoderma lucidum cultivation, in particular to a cultivation method of ganoderma lucidum spore powder with high triterpene content.
Background
The ganoderma triterpene is a triterpenoid found in ganoderma, is a scientific name of ganoderma total triterpene and is a main chemical and medicinal component of ganoderma, and the ganoderma triterpene contained in the ganoderma has particularly remarkable physiological activity.
Ganoderma triterpene is important pharmacological component of Ganoderma, and triterpene component in Ganoderma plays important role in resisting tumor.
Artificial cultivation of ganoderma lucidum is prosperous, and the polysaccharide content and the triterpene content of high spore powder mainly prepared by harvested spores are main indexes for measuring the quality of medicinal ganoderma lucidum. In particular, the ganoderma lucidum spore powder with high triterpene content has higher medicinal value.
The main factors influencing the cultivation of the lucid ganoderma include culture materials, varieties, soil, water, climate, microenvironment, growth period and the like; at present, no cultivation method for improving the content of triterpenoids in ganoderma lucidum spore powder is available.
Disclosure of Invention
In order to improve the triterpene content of the ganoderma lucidum spore powder, the invention provides a cultivation method for the ganoderma lucidum spore powder with high triterpene content.
The technical scheme of the invention is as follows: the method for cultivating the ganoderma lucidum spore powder with high content of the triterpene comprises the following specific steps:
(1) the culture process comprises the following steps: filling the culture material into a bag of 8-9Kg, tying two bag openings tightly by slipknots, conveying to a sterilization chamber, and sterilizing for 24-35 hours at the temperature of 100-; cooling to below 30 deg.C, inoculating Ganoderma strain to obtain stick; after inoculation, the fungus sticks are placed in a fungus growing bank for culture for 50-70 days;
(2) selecting an environment of a fruiting place: 31-33 degrees north latitude, elevation 900-; planting requirements are as follows: ditching 18-22cm deep and 35-40cm wide along the tree under the fruiting field, transporting the fungus sticks full of hyphae cultured in the step (1) to the fruiting field, transversely placing the fungus sticks, keeping a distance of 8-12cm between the fungus sticks, then covering soil, and entering the ganoderma lucidum growth management process;
(3) the spore powder collecting cover is arranged: arranging two layers of mulching films on the surface of a ganoderma ridge after soil covering, cutting a small hole at the top of the ganoderma to expose the ganoderma head after the ganoderma grows out, only leaving one ganoderma head on each fungus stick, arranging a white non-woven fabric spore powder collecting cover on each mulching film before at least a quantity of spore powder of the ganoderma grows out, clamping the edge of the upper layer of mulching film and the edge of the bottom of a white non-woven fabric spore powder collecting cover, arranging a small arched shed, and covering a transparent film with the thickness of 0.4-0.5 mm on the small arched shed;
(4) managing the powder collecting period:
the film can not be opened at other time unless the temperature is higher and the two ends of the transparent film need to be opened for ventilation and temperature reduction, so as to prevent the spore powder from losing; when the spore powder is continuously emitted for 90-120 days and the ganoderma lucidum grows into the decline stage, harvesting the spore powder in sunny days and harvesting the ganoderma lucidum fruiting body at the same time.
The ganoderma lucidum strain is optimally selected from Shanghai agricultural science institute 'Shanghai ganoderma lucidum No. 1'.
The invention has the beneficial effects that: according to the invention, the specific latitude range and altitude range are selected as the growing environment of the ganoderma lucidum, and the produced ganoderma lucidum spore powder can obviously improve the content of effective medicinal components such as polysaccharide, triterpene and the like.
Detailed Description
The present invention will be described in further detail with reference to examples.
Example 1
The method for cultivating the ganoderma lucidum spore powder with high content of the triterpene comprises the following specific steps:
(1) the culture process comprises the following steps: filling the culture material into 8.5Kg of a bag, tying two bag openings tightly by slipknots, conveying to a sterilization chamber, and sterilizing at 100 ℃ for 24 hours; cooling to below 30 deg.C, inoculating Ganoderma strain (Shanghai agricultural science institute, Hunong Ganoderma No. 1) to obtain stick; after inoculation, the fungus sticks are placed in a fungus growing bank for culture for 60 days;
(2) selecting an environment of a fruiting place: the method comprises the following steps of (1) marking lake forest farms in Zhuxi county, 32.2 degrees in northern latitude, 1080 m in altitude, 98% in forest coverage of cedar, and 3-5 cm in deciduous humus soil; planting requirements are as follows: ditching 20cm deep and 40cm wide along the tree under the fruiting land, transporting the fungus sticks full of hyphae cultured in the step (1) to the fruiting land, transversely placing the fungus sticks with a distance of 8-12cm between the fungus sticks, covering soil, and entering the ganoderma lucidum growth management process;
(3) the spore powder collecting cover is arranged: arranging two layers of mulching films on the surface of a ganoderma ridge after soil covering, cutting a small hole at the top of the ganoderma to expose the ganoderma head after the ganoderma grows out, only leaving one ganoderma head on each fungus stick, arranging a white non-woven fabric spore powder collecting cover on each mulching film before at least a quantity of spore powder of the ganoderma grows out, clamping the edge of the upper layer of mulching film and the edge of the bottom of a white non-woven fabric spore powder collecting cover, arranging a small arched shed, and covering a transparent film with the thickness of 0.4-0.5 mm on the small arched shed;
(4) managing the powder collecting period:
the film can not be opened at other time unless the temperature is higher and the two ends of the transparent film need to be opened for ventilation and temperature reduction, so as to prevent the spore powder from losing; when the spore powder is continuously emitted for 120 days and the ganoderma lucidum grows into a decline stage, harvesting the spore powder in sunny days and harvesting the ganoderma lucidum fruiting body at the same time.
In example 1, the yield is determined, and the content of the ganoderma lucidum spore powder polysaccharide is 2.54 percent and the content of the total triterpene of the ganoderma lucidum spore powder is 5.68 percent, which are determined by Zhejiang Wuyangtang medicine Limited company.
Comparative example 2
The method for cultivating the ganoderma lucidum spore powder with high content of the triterpene comprises the following specific steps:
(1) the culture process comprises the following steps: filling the culture material into 8.5Kg of a bag, tying two bag openings tightly by slipknots, conveying to a sterilization chamber, and sterilizing at 100 ℃ for 24 hours; cooling to below 30 deg.C, inoculating Ganoderma strain (Shanghai agricultural science institute, Hunong Ganoderma No. 1) to obtain stick; after inoculation, the fungus sticks are placed in a fungus growing bank for culture for 60 days;
(2) selecting an environment of a fruiting place: tuiyichun, Zhuxi county, 32.18 degrees north latitude, 455.6 meters elevation, non-forest, 65% sunshade net; planting requirements are as follows: ditching 20cm deep and 40cm wide along the tree under the fruiting land, transporting the fungus sticks full of hyphae cultured in the step (1) to the fruiting land, transversely placing the fungus sticks with a distance of 8-12cm between the fungus sticks, covering soil, and entering the ganoderma lucidum growth management process;
(3) the spore powder collecting cover is arranged: arranging two layers of mulching films on the surface of a ganoderma ridge after soil covering, cutting a small hole at the top of the ganoderma to expose the ganoderma head after the ganoderma grows out, only leaving one ganoderma head on each fungus stick, arranging a white non-woven fabric spore powder collecting cover on each mulching film before at least a quantity of spore powder of the ganoderma grows out, clamping the edge of the upper layer of mulching film and the edge of the bottom of a white non-woven fabric spore powder collecting cover, arranging a small arched shed, and covering a transparent film with the thickness of 0.4-0.5 mm on the small arched shed;
(4) managing the powder collecting period:
the film can not be opened at other time unless the temperature is higher and the two ends of the transparent film need to be opened for ventilation and temperature reduction, so as to prevent the spore powder from losing; when the spore powder is continuously emitted for 120 days and the ganoderma lucidum grows into a decline stage, harvesting the spore powder in sunny days and harvesting the ganoderma lucidum fruiting body at the same time.
In comparative example 2, the yield is determined, and the content of the ganoderma lucidum spore powder polysaccharide is 2.16% and the content of the ganoderma lucidum spore powder triterpene is 4.17% by the Zhejiang Wuyangtang medicine Limited company.
By the embodiment 1 and the comparative example 2, the total triterpene content of the ganoderma lucidum spore powder polysaccharide which grows in the same batch of mushroom wood under different site environments (altitude) under the condition of basically the same latitude is obviously different. Therefore, the method for producing the ganoderma lucidum spore powder in the specific environment can obviously improve the content of effective medicinal components such as polysaccharide, triterpene and the like.
Claims (2)
1. The method for cultivating the ganoderma lucidum spore powder with high content of the triterpene is characterized by comprising the following specific steps:
(1) the culture process comprises the following steps: filling the culture material into a bag of 8-9Kg, tying two bag openings tightly by slipknots, conveying to a sterilization chamber, and sterilizing for 24-35 hours at the temperature of 100-; cooling to below 30 deg.C, inoculating Ganoderma strain to obtain stick; after inoculation, the fungus sticks are placed in a fungus growing bank for culture for 50-70 days;
(2) selecting an environment of a fruiting place: 31-33 degrees north latitude, elevation 900-; planting requirements are as follows: ditching 18-22cm deep and 35-40cm wide along the tree under the fruiting field, transporting the fungus sticks full of hyphae cultured in the step (1) to the fruiting field, transversely placing the fungus sticks, keeping a distance of 8-12cm between the fungus sticks, then covering soil, and entering the ganoderma lucidum growth management process;
(3) the spore powder collecting cover is arranged: arranging two layers of mulching films on the surface of a ganoderma ridge after soil covering, cutting a small hole at the top of the ganoderma to expose the ganoderma head after the ganoderma grows out, only leaving one ganoderma head on each fungus stick, arranging a white non-woven fabric spore powder collecting cover on each mulching film before at least a quantity of spore powder of the ganoderma grows out, clamping the edge of the upper layer of mulching film and the edge of the bottom of a white non-woven fabric spore powder collecting cover, arranging a small arched shed, and covering a transparent film with the thickness of 0.4-0.5 mm on the small arched shed;
(4) managing the powder collecting period:
the film can not be opened at other time unless the temperature is higher and the two ends of the transparent film need to be opened for ventilation and temperature reduction, so as to prevent the spore powder from losing; when the spore powder is continuously emitted for 90-120 days and the ganoderma lucidum grows into the decline stage, harvesting the spore powder in sunny days and harvesting the ganoderma lucidum fruiting body at the same time.
2. The method for cultivating ganoderma lucidum spore powder with high triterpene content according to claim 1, wherein the ganoderma lucidum strain is Shanghai agricultural science institute "Shanghai nong ganoderma lucidum No. 1".
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111620857.XA CN114027093A (en) | 2021-12-28 | 2021-12-28 | Method for cultivating ganoderma lucidum spore powder with high content of triterpene |
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| CN202111620857.XA CN114027093A (en) | 2021-12-28 | 2021-12-28 | Method for cultivating ganoderma lucidum spore powder with high content of triterpene |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115039638A (en) * | 2022-04-22 | 2022-09-13 | 云南省农业科学院生物技术与种质资源研究所 | Ganoderma resinatum strain H63 and application thereof |
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2021
- 2021-12-28 CN CN202111620857.XA patent/CN114027093A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115039638A (en) * | 2022-04-22 | 2022-09-13 | 云南省农业科学院生物技术与种质资源研究所 | Ganoderma resinatum strain H63 and application thereof |
| CN115039638B (en) * | 2022-04-22 | 2023-12-29 | 云南省农业科学院生物技术与种质资源研究所 | Resin ganoderma lucidum strain H63 and application thereof |
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Application publication date: 20220211 |