CN113994902B - Method for keep-alive transportation of golden pomfret - Google Patents
Method for keep-alive transportation of golden pomfret Download PDFInfo
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- CN113994902B CN113994902B CN202111285195.5A CN202111285195A CN113994902B CN 113994902 B CN113994902 B CN 113994902B CN 202111285195 A CN202111285195 A CN 202111285195A CN 113994902 B CN113994902 B CN 113994902B
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- 241000630524 Taractes rubescens Species 0.000 title claims abstract description 92
- 238000000034 method Methods 0.000 title claims abstract description 21
- 239000013535 sea water Substances 0.000 claims abstract description 56
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims abstract description 42
- 206010002091 Anaesthesia Diseases 0.000 claims abstract description 27
- 230000037005 anaesthesia Effects 0.000 claims abstract description 27
- 244000223014 Syzygium aromaticum Species 0.000 claims abstract description 24
- 239000000341 volatile oil Substances 0.000 claims abstract description 24
- 235000016639 Syzygium aromaticum Nutrition 0.000 claims abstract description 23
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims abstract description 21
- 229930003268 Vitamin C Natural products 0.000 claims abstract description 21
- 235000019154 vitamin C Nutrition 0.000 claims abstract description 21
- 239000011718 vitamin C Substances 0.000 claims abstract description 21
- 241000276694 Carangidae Species 0.000 claims abstract description 17
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 16
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 16
- 239000001301 oxygen Substances 0.000 claims abstract description 16
- 238000012258 culturing Methods 0.000 claims abstract description 10
- 235000013305 food Nutrition 0.000 claims description 5
- 230000001706 oxygenating effect Effects 0.000 claims description 2
- 238000007789 sealing Methods 0.000 claims 1
- 230000004083 survival effect Effects 0.000 abstract description 31
- 230000001681 protective effect Effects 0.000 abstract description 25
- 230000002035 prolonged effect Effects 0.000 abstract description 6
- 230000008901 benefit Effects 0.000 abstract description 5
- 239000007788 liquid Substances 0.000 abstract description 4
- 230000008569 process Effects 0.000 abstract description 4
- 230000005059 dormancy Effects 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 abstract description 2
- 241000628997 Flos Species 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 13
- 241000251468 Actinopterygii Species 0.000 description 11
- 238000002474 experimental method Methods 0.000 description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 10
- 238000011084 recovery Methods 0.000 description 8
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 230000000384 rearing effect Effects 0.000 description 3
- 230000036387 respiratory rate Effects 0.000 description 3
- 241000719209 Trachinotus ovatus Species 0.000 description 2
- 238000001949 anaesthesia Methods 0.000 description 2
- 230000003444 anaesthetic effect Effects 0.000 description 2
- 230000006399 behavior Effects 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 230000003542 behavioural effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000010634 clove oil Substances 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 230000020477 pH reduction Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 235000014102 seafood Nutrition 0.000 description 1
- QJVXKWHHAMZTBY-GCPOEHJPSA-N syringin Chemical compound COC1=CC(\C=C\CO)=CC(OC)=C1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 QJVXKWHHAMZTBY-GCPOEHJPSA-N 0.000 description 1
- QJVXKWHHAMZTBY-KSXIZUIISA-N syringin Natural products COc1cc(C=CCO)cc(OC)c1O[C@H]2O[C@@H](CO)[C@H](O)[C@@H](O)[C@@H]2O QJVXKWHHAMZTBY-KSXIZUIISA-N 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a method for keep-alive transportation of golden pompano, which comprises the following steps: stopping feeding the fresh golden pomfret in fresh seawater for temporarily culturing; placing golden pomfret in seawater containing flos Caryophylli essential oil for anesthesia; transferring golden pomfret into a sealed bag, and adding a protective solution containing vitamin C; the sealed bag is oxygenated and is placed in a transport box for transportation. Compared with the existing golden pomfret transportation technology in the market, the method has the advantages that the golden pomfret dormancy is induced by stopping feeding and temporarily culturing, the clove essential oil at low temperature, the golden pomfret is kept alive and transported under the conditions of sufficient oxygen and protection liquid addition, the stress reaction of the golden pomfret in the keep alive transportation is relieved, the loading capacity is increased by 5-10 times, the transportation time is prolonged, the transportation cost is obviously reduced, and the survival rate of the golden pomfret in the transportation process is increased. The technical scheme provided by the invention has great significance for improving the economic benefit of the golden pompano market.
Description
Technical Field
The invention relates to the field of keep-alive transportation of aquatic products, in particular to a keep-alive transportation method of golden pompano.
Background
Golden pompano (Golden pompano) is a bass order named as Oval pompano (Oval pompano), also called yellow wax fish, with the body length being about 2 times of the body height. Golden pomfret is a warm water-borne middle and upper layer fish, and has extremely rich protein content of about 15.6 percent. Golden pomfret meat is tender, delicious and is a rare edible fish. Golden pomfret has weak low temperature resistance, stops eating when the temperature is reduced to below 16 ℃, dies at the temperature below 14 ℃, and adapts to salinity of 2-25 per mill. Chinese golden pomfret is mainly cultivated in the south of the sea and two broad areas, and has a plurality of large-scale cultivation bases, and the artificial cultivation yield is about 17 ten thousand tons per year.
As the golden pomfret has high culture yield, concentrated harvest, strong stress property and low temperature resistance, the golden pomfret is mainly sold in the form of frozen fresh products in the market at present, and the price of the fresh products is higher. This results in an increase in the cost of golden pomfret, without taking advantage of the development of its sales market.
Therefore, the method for keeping alive and transporting the golden pomfret is high in density and survival rate, and has important significance for market development of the golden pomfret.
Disclosure of Invention
The invention aims to provide a method for keep-alive transportation of golden pompanos, which aims to improve the survival rate of the golden pompanos in the keep-alive transportation process, improve the transportation loading capacity and reduce the transportation line.
In order to achieve the purpose, the invention is realized by the following scheme:
a method for keep-alive transportation of golden pomfret is characterized by comprising the following steps:
s1, stopping feeding and temporarily breeding live golden pomfret in fresh seawater at the temperature of 20-25 ℃ for 6-12 hours;
s2, placing the golden pomfret in seawater containing 20-40 mg/L clove essential oil at 15-20 ℃ for anesthesia;
s3, transferring golden pompano into a sealed bag, and adding seawater, wherein the volume-mass ratio of the seawater to the golden pompano is (0.5-2);
s4, oxygenating the sealed bag, and transporting at 15-18 ℃.
Preferably, in the step S1, the seawater is fresh seawater containing 10-30 mg/L vitamin C, the temperature is 25 ℃, the salinity is 20-25 per mill, the dissolved oxygen is 7.0-8.0 mg/L, and the time for stopping food and temporarily culturing is 6h.
Preferably, in step S2, golden pompano is anesthetized in seawater of 20 ℃ containing 40mg/L clove essential oil.
Preferably, in step S2, the clove essential oil is an extract of the plant clove flower and leaf.
Preferably, in step S3, the volume-to-mass ratio of the seawater to the golden pompano is 2.
Preferably, in the step S3, the seawater is fresh seawater containing 10-30 mg/L of vitamin C, the temperature of the seawater is 15 ℃, the salinity is 20-25 per mill, the dissolved oxygen is 6.5-7.5 mg/L.
More preferably, in step S3, the seawater contains 10mg/L vitamin C.
Preferably, in step S4, the product is transported in a transport box at 15-18 ℃.
More preferably, in step S4, the sealed bag is oxygenated and placed in a transport box at 15-18 ℃ for 12-14 h at the longest.
Compared with the prior art, the invention has the following beneficial effects:
compared with the existing golden pomfret transportation technology in the market, the method has the advantages that the golden pomfret dormancy is induced by stopping feeding and temporarily culturing, the clove essential oil at low temperature, the golden pomfret is kept alive and transported under the conditions of sufficient oxygen and protective liquid addition, the keep-alive transportation stress reaction of the golden pomfret is relieved, the loading capacity is improved by 5-10 times, the transportation time is prolonged, the transportation cost is obviously reduced, and the survival rate of the golden pomfret in the transportation process is improved. The technical scheme provided by the invention has great significance for improving the economic benefit of the golden pomfret market.
Detailed Description
The present invention is further described in detail in the following description and specific examples, which are provided for the purpose of illustration only and are not intended to limit the scope of the present invention. The test methods used in the following examples are all conventional methods unless otherwise specified; the materials, reagents and the like used are, unless otherwise specified, commercially available reagents and materials.
Golden pomfret used in the invention is purchased from the seafood wholesale market in Zhanjiang city, and the golden pomfret in the wholesale market is mainly from the golden pomfret cultivation base in Hainan.
Reagent: vitamin C is purchased from Solarbio (cat # A8100), and clove essential oil is food grade (CAS # 8000-34-8).
The live-keeping transportation system is adopted for transportation, and is provided with a spiral-flow type protein separator, a biological mechanical filter, a refrigerator and a PP temporary culture pond with the volume of 1 cubic meter.
Example 1 Effect of feed stopping and temporary rearing time on the survival time of golden pomfret with water
1. Experimental methods
(1) Selecting 50 golden pomfret (average weight of 350 g) with consistent growth vigor, randomly dividing into 5 groups, and placing 10 golden pomfret in a keep-alive transportation system, and respectively stopping feeding and temporarily culturing for 0h, 6h, 12h, 24h and 48h in 330L natural purified seawater with 25 ℃, 25 per thousand salinity and dissolved oxygen of 7.0-8.0 mg/L.
(2) Preparing a protective solution: fresh seawater at 15 ℃, 20-25 per mill of salinity, 6.5-7.5 mg/L of dissolved oxygen and 10mg/L of vitamin C.
(3) Placing golden pomfret in seawater containing 40mg/L clove essential oil at 20 ℃ for anesthesia for 10s, taking out, transferring into a sealed bag, and adding a protective solution, wherein the volume-mass ratio of the protective solution to the pomfret is 2.
(4) Timing from the end of the oxygen-filling package of the keep-alive bag, putting the keep-alive bag into a keep-alive transportation system for anhydrous transportation for 12h, putting the golden pomfret into 20 ℃ seawater for recovery, and continuously filling air into the seawater in the recovery process. Observing every 2h for 1 time, taking the non-response of the stimulated fish body as a death characteristic, and recording and counting the survival time of the golden pomfret.
2. Results of the experiment
The statistical results of the survival time of golden pomfret with water transportation under different temporary rearing time are shown in table 1.
TABLE 1 influence of different temporary rearing periods on the survival time of golden pompano in water transportation
The results show that after the golden pomfret is fed for 6 hours temporarily, the golden pomfret is anesthetized in seawater containing 40mg/L clove essential oil for 10s at 20 ℃, the survival time reaches 38.5 hours under the condition of containing 2 times of volume of protection liquid, and the survival transportation time is prolonged by 20% compared with the survival transportation time under the condition of not feeding temporarily. Comprehensively considering, the optimal time length for stopping food and temporarily culturing golden pomfret is 6h.
Example 2 Effect of different anaesthesia methods on the waterless survival time of golden pomfret
1. Experimental methods
(1) 24 golden pomfret (average weight of 350 g) with consistent growth vigor is selected, randomly divided into 3 groups with 8 fishes in each group, and the golden pomfret is put into a keep-alive transportation system and is stopped to be temporarily cultured for 6 hours in 330L natural purified seawater with 25 ℃, 25 per thousand of salinity and 7.0-8.0 mg/L of dissolved oxygen.
(2) Preparing a protective solution: fresh seawater at 15 ℃, 20-25 per mill of salinity, 6.5-7.5 mg/L of dissolved oxygen and 10mg/L of vitamin C.
(3) Placing 3 groups of golden pomfret in seawater (oleum Caryophylli anesthesia) containing 40mg/L oleum Caryophylli at 20 deg.C and 125mg/LCO at 18 deg.C 2 Seawater (CO) 2 Anesthesia) and anesthesia in seawater at 15 deg.C for 10s (ice temperature anesthesia), taking out, transferring into a sealed bag,and adding a protective solution, wherein the volume-mass ratio of the protective solution to the fish body is 2.
(4) Timing from the end of the oxygen-filling package of the keep-alive bag, putting the keep-alive bag into a keep-alive transportation system for anhydrous transportation for 12h, putting the golden pomfret into 20 ℃ seawater for recovery, and continuously filling air into the seawater in the recovery process. Observing every 2h for 1 time, taking the non-response of the stimulated fish body as a death characteristic, and observing and counting the survival time of the golden pomfret.
2. Results of the experiment
The statistics of the anhydrous survival time of golden pomfret under different anesthesia methods are shown in table 2.
TABLE 2 Effect of different anaesthesia methods on the survival time of golden pomfret without water
The results show that after seawater containing 40mg/L clove essential oil at 20 ℃ is adopted to anaesthetize the golden pomfret, the survival time reaches 14h under the condition of 2 times of volume of protective liquid, which is higher than that of the golden pomfret containing 125mg/LCO at 18 DEG C 2 The keep-alive time under the seawater anesthesia condition is prolonged by 16 percent; the survival time is prolonged by 29 percent compared with the survival time under the anesthesia condition in the seawater at the temperature of 15 ℃. And the temperature is 20 ℃ under the anesthesia condition, so that the cost of cooling the water body is obviously saved.
Example 3 Effect of different clove essential oil concentrations and seawater temperatures on the anesthetic effect of Trachinotus ovatus
1. Experimental methods
(1) Selecting 70 golden pomfret fishes with consistent growth vigor (average weight is 350 g), randomly dividing into 7 groups, and putting the golden pomfret fishes into a keep-alive transportation system, and stopping feeding and temporarily culturing for 6h in 330L natural purified seawater with 25 ℃ and 25 per thousand of salinity and 7.0-8.0 mg/L of dissolved oxygen.
(2) Preparing a protective solution: fresh seawater at 15 ℃, 20-25 per mill of salinity, 6.5-7.5 mg/L of dissolved oxygen and 10mg/L of vitamin C.
(3) 7 groups of golden pomfret are respectively placed in seawater containing 20-80 mg/L clove essential oil at 20 ℃ and seawater containing 40mg/L clove essential oil at 15-25 ℃ for anesthesia.
(4) According to the respiratory rate and behavior characteristics (A represents anesthesia and R represents Resuscitation) in tables 3-4, the time when the anesthesia of golden pomfret starts to reach the A4 stage is recorded as the anesthesia time, and the time when golden pomfret recovers from the A4 stage to the R4 stage is recorded as the Resuscitation time.
TABLE 3 respiratory rate and behavioral characteristics of Trachinotus ovatus during anesthesia period
TABLE 4 respiratory rate and behavior characteristics of resuscitated period of golden pomfret
2. Results of the experiment
(1) The golden pomfret was anesthetized with clove oil at various concentrations, and the statistics of anesthesia time and resuscitation time are shown in table 5.
TABLE 5 anesthetic effect of different concentrations of clove essential oil on golden pomfret
The statistical results in table 5 show that in the case of water temperature of 20 ℃, when the concentration of clove essential oil is 20-60 mg/L, the higher the concentration of clove essential oil is, the shorter the anesthesia time is, and the longer the resuscitation time is. When the concentration of syringin is 40mg/L and 80mg/L, the anesthesia time and the resuscitation time have no significant difference (P is more than 0.05). Comprehensively considering, the concentration of clove essential oil with the best effect of anaesthetizing golden pomfret is 40mg/L.
(2) The concentration of clove essential oil is 40mg/L, and the statistical results of the anesthesia time and the resuscitation time of the golden pomfret at different temperatures are shown in Table 6.
TABLE 6 anesthesia effect of different temperatures on golden pomfret
The statistical results in table 6 show that when the water temperature is 15-25 ℃, the anesthesia time of the golden pomfret is reduced firstly and then increased along with the increase of the water temperature, and the resuscitation time is reduced continuously. Comprehensively considering, when the concentration of clove essential oil is 40mg/L, the water temperature with the best effect of anaesthetizing golden pomfret is 20 ℃, so that golden pomfret can be completely anaesthetized within 3min and completely revived within 5 min.
Example 4 Effect of protective solutions with different vitamin C concentrations on the keep-alive effect of golden pomfret
1. Experimental methods
(1) Selecting 40 golden pomfret fishes (with the average weight of 350 g) with consistent growth vigor, randomly dividing into 4 groups, 10 fishes in each group, putting the golden pomfret fishes into a keep-alive transportation system, and stopping feeding and temporarily culturing for 6 hours in 330L natural purified seawater with the salinity of 25 per thousand and the dissolved oxygen of 7.0-8.0 mg/L at the temperature of 25 ℃.
(2) Preparing a protective solution: fresh seawater at 15 ℃, 20 to 25 per mill of salinity, 6.5 to 7.5mg/L of dissolved oxygen and 0 to 30mg/L of vitamin C.
(3) Respectively placing 4 groups of golden pomfret in seawater containing 40mg/L clove essential oil at 20 ℃ for anesthesia for 10s, taking out, transferring into a sealed bag, and adding a protection solution, wherein the volume mass ratio of the protection solution to the pomfret is 2.
(4) Timing from the end of the oxygen-filling package of the keep-alive bag, putting the keep-alive bag into a keep-alive transportation system for 14h of anhydrous transportation, putting the golden pomfret into 20 ℃ seawater for recovery, and continuously filling air into the seawater in the recovery process. Observing for 1 time every 1h, taking the unresponsiveness of the stimulated fish body as a death characteristic, observing and counting the survival time of the golden pomfret in the keep-alive transportation process, and counting the survival rate.
2. Results of the experiment
The golden pomfret was transported waterless using protection solutions containing different concentrations of vitamin C, and the survival time and survival rate of golden pomfret were statistically shown in table 7.
TABLE 7 Effect of vitamin C concentration in protective solution on survival time of golden pomfret during keep-alive transportation
The statistical results in table 7 show that after the golden pomfret is anesthetized by seawater containing 40mg/L clove essential oil at 20 ℃, the survival time and survival rate of the golden pomfret in live keeping transportation are remarkably improved by using the protective solution containing vitamin C compared with the protective solution containing no vitamin C. When the concentration of vitamin C in the protective solution is 10mg/L, the survival time of the golden pomfret reaches 16h, and is prolonged by 12 times compared with the survival transportation survival time of the protective solution without the vitamin C. Along with the increase of the concentration of the vitamin C in the protective solution, the keep-alive transportation time has a remarkable descending trend. This is related to the stress of fish caused by acidification of water body due to too high concentration of vitamin C.
Example 5 Effect of different amounts of protective solution on the keep-alive effect of golden pomfret
1. Experimental methods
(1) Selecting 40 golden pomfret fishes (with the average weight of 350 g) with consistent growth vigor, randomly dividing into 4 groups, 10 fishes in each group, putting the golden pomfret fishes into a keep-alive transportation system, and stopping feeding and temporarily culturing for 6 hours in 330L natural purified seawater with the salinity of 25 per thousand and the dissolved oxygen of 7.0-8.0 mg/L at the temperature of 25 ℃.
(2) Preparing a protective solution: fresh seawater at 15 ℃, 20 to 25 per mill of salinity, 6.5 to 7.5mg/L of dissolved oxygen and 0 to 30mg/L of vitamin C.
(3) Respectively placing 4 groups of golden pomfret in seawater containing 40mg/L clove essential oil at 20 ℃ for anesthesia for 10s, taking out, transferring into a sealed bag, and adding a protective solution, wherein the volume-mass ratio of the protective solution to the pomfret is 0-4.
(4) Timing from the end of the oxygen-filling package of the keep-alive bag, putting the keep-alive bag into a keep-alive transportation system for waterless transportation for 3h, putting the golden pomfret into 20 ℃ seawater for recovery, and continuously filling air into the seawater in the recovery process. The survival rate of the golden pomfret is observed and counted, and the result is shown in the table 8.
2. Results of the experiment
The statistical results of the survival time of golden pomfret during keep-alive transportation and the survival rate of golden pomfret after keep-alive transportation under different addition amounts of the protective solution are shown in table 8.
TABLE 8 keep-alive effect of different addition amounts of protective solution on golden pompano
The statistical results in table 8 show that when the volume mass ratio of the protective solution to golden pomfret is 2.
It should be finally noted that the above examples are only intended to illustrate the technical solutions of the present invention and not to limit the scope of the present invention, and that those skilled in the art can make other variations or modifications on the basis of the above description and idea, and that all embodiments are neither necessary nor exhaustive. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.
Claims (5)
1. A method for keep-alive transportation of golden pomfret is characterized by comprising the following steps:
s1, stopping feeding and temporarily culturing fresh golden pomfret in fresh seawater for 6-12 h, wherein the fresh seawater is fresh seawater with the temperature of 20-25 ℃, the salinity of 20-25 per mill and the dissolved oxygen amount of 7.0-8.0 mg/L,
s2, placing the golden pomfret in seawater containing 20-40 mg/L clove essential oil at 15-20 ℃ for anesthesia;
s3, transferring the golden pompano into a sealing bag, and adding seawater, wherein the volume-mass ratio of the seawater to the golden pompano is 2; the seawater is fresh seawater which has the temperature of 15 ℃, the salinity of 20-25 per mill, the dissolved oxygen amount of 6.5-7.5 mg/L and contains 10-30 mg/L of vitamin C;
s4, oxygenating the sealed bag, and transporting at 15-18 ℃.
2. The method according to claim 1, wherein the time for stopping food and temporarily keeping food in step S1 is 6h.
3. The method according to claim 1, wherein in step S2, the content of the clove essential oil is 40mg/L.
4. The method according to claim 1, wherein the temperature of the seawater in step S2 is 20 ℃.
5. The method according to claim 1, wherein the seawater contains 10mg/L vitamin C in step S3.
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| EP3243381A1 (en) * | 2016-05-10 | 2017-11-15 | An Fheirm Choireil Teoranta | A container for transporting livestock for an aquarium |
| CN106069945A (en) * | 2016-07-05 | 2016-11-09 | 临高海丰养殖发展有限公司 | A kind of gold Stromateoides argenteus cultural method |
| CN108719147A (en) * | 2018-05-25 | 2018-11-02 | 广东海洋大学 | A kind of long-distance transport method of seawater grouper |
| CN211322633U (en) * | 2019-10-22 | 2020-08-25 | 薛栋 | A conveyer for golden pomfret |
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