CN113943640A - Biological particle trapping experimental equipment and application thereof - Google Patents
Biological particle trapping experimental equipment and application thereof Download PDFInfo
- Publication number
- CN113943640A CN113943640A CN202111212214.1A CN202111212214A CN113943640A CN 113943640 A CN113943640 A CN 113943640A CN 202111212214 A CN202111212214 A CN 202111212214A CN 113943640 A CN113943640 A CN 113943640A
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- China
- Prior art keywords
- liquid medium
- pipe
- biological particle
- biological
- hole piston
- Prior art date
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- Pending
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- 239000002245 particle Substances 0.000 title claims abstract description 30
- 239000012528 membrane Substances 0.000 claims abstract description 17
- 238000001914 filtration Methods 0.000 claims abstract description 12
- 238000007789 sealing Methods 0.000 claims abstract description 7
- 239000007788 liquid Substances 0.000 claims description 16
- 238000000034 method Methods 0.000 claims description 10
- 230000005484 gravity Effects 0.000 claims description 3
- 239000000565 sealant Substances 0.000 claims description 3
- 239000010902 straw Substances 0.000 claims description 3
- 238000002474 experimental method Methods 0.000 claims 2
- 230000003068 static effect Effects 0.000 abstract description 5
- 230000000295 complement effect Effects 0.000 abstract description 3
- 230000002380 cytological effect Effects 0.000 abstract description 3
- 239000012530 fluid Substances 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 2
- 210000001772 blood platelet Anatomy 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 239000007787 solid Substances 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000012472 biological sample Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 210000005056 cell body Anatomy 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 230000007257 malfunction Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
Images
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/02—Separating microorganisms from the culture medium; Concentration of biomass
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/508—Containers for the purpose of retaining a material to be analysed, e.g. test tubes rigid containers not provided for above
- B01L3/5082—Test tubes per se
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/0681—Filter
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2400/00—Moving or stopping fluids
- B01L2400/04—Moving fluids with specific forces or mechanical means
- B01L2400/0475—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure
- B01L2400/0478—Moving fluids with specific forces or mechanical means specific mechanical means and fluid pressure pistons
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/4077—Concentrating samples by other techniques involving separation of suspended solids
- G01N2001/4088—Concentrating samples by other techniques involving separation of suspended solids filtration
Abstract
The invention relates to the field of experimental equipment, in particular to biological particle trapping experimental equipment and application thereof. The technical scheme of the invention is as follows: a biological particle trapping experimental device comprises a collecting pipe, a filtering membrane, a through hole piston, a connecting pipe, a suction pipe and a sealing cover. The invention does not need a complex control structure, can replace the complementary set device of the prior cytological analysis system, and the working environment can adapt to dynamic and static fluids.
Description
Technical Field
The invention relates to the field of scientific research and teaching equipment, in particular to biological particle trapping experimental equipment and application thereof.
Background
In the field of medical diagnostics and more particularly in cancer diagnostics, there are numerous methods and experimental facilities for preparing biological samples for subsequent cytological analysis, but at present the various electronic regulation devices contained in these experimental facilities are complex, which significantly increase the risk of malfunctions or even the risk of stoppage within the malfunctioning system, and in addition, the very complex automated systems are expensive to purchase, and CN102165303A specification CN102165306a2/15 is also expensive due to the need for secondary settings and regular maintenance by professionals, increasing the costs of scientific teaching, not favouring the normal performance of the teaching.
Bioparticle trapping is a common method of cytological diagnosis because such techniques enable the detection of diseases associated with vital organ prognosis.
The device described in the invention patent application CN102165303a provides a biological particle capture experimental device using a method of immersion in a static liquid medium containing suspended biological particles, but the device can only be used in a static liquid medium and cannot be applied to a dynamic liquid medium.
Disclosure of Invention
The invention aims to provide biological particle trapping experimental equipment to solve the problems that the existing equipment is complex in structure, high in manufacturing cost, not beneficial to scientific research and teaching activities, only can be used for a static liquid medium and cannot be applied to a dynamic liquid medium.
The invention is realized by the following steps:
the utility model provides a biological particle entrapment experimental facilities, includes collecting pipe, filtration membrane, through-hole piston, connecting pipe, straw and sealed lid:
the top end of the collecting pipe is connected with the suction pipe,
the through hole piston is arranged in the collecting pipe and is connected with the inner side wall of the collecting pipe in a sliding way,
the filter membrane is positioned at the top end of the through hole piston,
the top end and the bottom end of the connecting pipe are both open, the upper part of the connecting pipe extends into the collecting pipe and is fixedly connected with the bottom end of the through-hole piston,
the sealing cover is arranged at the bottom end of the connecting pipe.
The filter membrane is detachable.
The caliber of the suction pipe is 2-5 mm.
The connecting pipe and the bottom end of the through hole piston are fixedly connected by adopting a sealant.
The invention also relates to a method for trapping biological particles suspended in a liquid medium, in which the above-mentioned apparatus is used, comprising the following steps:
a) placing the pipette in a container containing a liquid medium in which biological particles are suspended;
b) sucking up the liquid medium;
c) and vertically placing one end of the suction pipe upwards, standing, and filtering the solution by gravity so as to trap the biological particles contained in the liquid medium on the outer surface of the filtering membrane.
In the step b, the method for sucking the liquid medium comprises the step of pulling the connecting pipe to form negative pressure in the collecting pipe through the piston, so that the solution medium is sucked into the collecting pipe.
The invention has the beneficial effects that:
the invention does not need a complex control structure, can replace the complementary collection device of the prior cytology analysis system, can adapt to dynamic and static fluids in working environment, can obtain cell preparations with quality at least equal to the known quality, has lower cost of teaching and scientific research equipment, and is convenient for developing scientific research and teaching activities.
Drawings
FIG. 1 is a vertical cross-sectional view of a biological particle capture experimental apparatus along its axis of symmetry before use;
FIG. 2 is a vertical cross-sectional view of the biological particle capture experimental apparatus in use, along its axis of symmetry, wherein the apparatus has been imbibed with a solution;
FIG. 3 is a schematic diagram of the structure of the present invention after being disassembled.
In the figure: 1. a collection pipe; 2. a filtration membrane; 3. a through-hole piston; 4. a connecting pipe; 5. a sealing cover; 6. a straw.
Detailed Description
The invention is further illustrated by the following examples, which are given by way of illustration only and are not intended to limit the scope of the invention in any way.
As shown in FIG. 1, the biological particle trapping experimental device comprises a collecting pipe 1, a filtering membrane 2, a through hole piston 3, a connecting pipe 4, a suction pipe 6 and a sealing cover 5.
The top end of the collecting pipe 1 is connected with a suction pipe 6.
The through hole piston 3 is positioned in the collecting pipe 1 and is connected in a sliding way.
The filter membrane 2 is positioned at the top end of the through-hole piston 3.
The connecting pipe 4 is fixedly connected with the bottom end of the through hole piston 3.
The sealing cover 5 is positioned at the bottom end of the connecting pipe 4.
Preferably, the caliber of the suction pipe 6 is 2-5 mm.
Preferably, the through-hole piston 3 may be made of any type of rubber.
Preferably, the filtering membrane 2 is detachably mounted, so that the filtering membrane is convenient to replace.
Preferably, the connecting pipe 4 is fixedly connected with the bottom end of the through hole piston 3 by adopting a sealant.
Biological particles mean any solid particles insoluble in aqueous solutions, including microorganisms, living cells, dead cells, anucleated cell bodies such as erythrocytes and platelets (thrombocytes), fragments, cell fragments and possibly crystals and light solid inclusions.
The invention can but not limited to complement biological particles from dynamic solution, and the using method comprises the following steps:
as shown in FIG. 2, the pipette 6 is immersed in the solution, the connecting tube 4 is pulled, a part of the solution is sucked into the collection tube 1 through the through-hole piston 3, the sealing cover 5 is opened, the pipette 6 of the present invention is vertically placed with one end facing upward and left standing, and the solution is filtered by gravity itself, which helps to effectively retain the biological particles on the outer surface of the filtration membrane 2 without destroying the physical integrity of the biological particles.
After the solution is filtered, the present invention is disassembled, the filter membrane 2 is brought into contact with the analysis vessel, and the biological particles on the filter membrane 2 are attached to the analysis vessel, as shown in fig. 3.
The above description of the present invention is intended to be illustrative. Various modifications, additions and substitutions for the specific embodiments described may be made by those skilled in the art without departing from the scope of the invention as defined in the accompanying claims.
Claims (6)
1. The utility model provides a biological particle entrapment experimental facilities which characterized in that, includes collecting pipe, filtration membrane, through-hole piston, connecting pipe, straw and sealed lid:
the top end of the collecting pipe is connected with the suction pipe,
the through hole piston is arranged in the collecting pipe and is connected with the inner side wall of the collecting pipe in a sliding way,
the filter membrane is positioned at the top end of the through hole piston,
the top end and the bottom end of the connecting pipe are both open, the upper part of the connecting pipe extends into the collecting pipe and is fixedly connected with the bottom end of the through-hole piston,
the sealing cover is arranged at the bottom end of the connecting pipe.
2. The biological particle capture experiment apparatus of claim 1, wherein the filter membrane is removable.
3. The biological particle capture experiment apparatus according to claim 1, wherein the diameter of the suction pipe is 2 to 5 mm.
4. The biological particle trapping experimental facility as claimed in claim 1, wherein said connecting tube is fixedly connected to the bottom end of said through-hole piston by a sealant.
5. A method for trapping biological particles suspended in a liquid medium, using the biological particle trapping experimental apparatus according to any one of claims 1 to 4, comprising the steps of:
a) placing the pipette in a container containing a liquid medium in which biological particles are suspended;
b) sucking up the liquid medium;
c) and vertically placing one end of the suction pipe upwards, standing, and filtering the solution by gravity so as to trap the biological particles contained in the liquid medium on the outer surface of the filtering membrane.
6. The method for trapping biological particles suspended in a liquid medium according to claim 5, wherein in the step b, the method for drawing the liquid medium comprises drawing the connecting tube to form a negative pressure in the collection tube by the piston, thereby drawing the solution medium into the collection tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111212214.1A CN113943640A (en) | 2021-10-18 | 2021-10-18 | Biological particle trapping experimental equipment and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111212214.1A CN113943640A (en) | 2021-10-18 | 2021-10-18 | Biological particle trapping experimental equipment and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113943640A true CN113943640A (en) | 2022-01-18 |
Family
ID=79331450
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111212214.1A Pending CN113943640A (en) | 2021-10-18 | 2021-10-18 | Biological particle trapping experimental equipment and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113943640A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110159533A1 (en) * | 2008-07-29 | 2011-06-30 | Bastien Karkouche | Bioparticle capture device, and use thereof |
| CN206736238U (en) * | 2017-05-09 | 2017-12-12 | 周口师范学院 | A kind of cell filter |
| CN109731107A (en) * | 2019-01-26 | 2019-05-10 | 临海市科晶生物科技有限公司 | A kind of compound disposable high-efficiency quick liquid sterilization purification device and its application method |
| CN210021758U (en) * | 2019-05-05 | 2020-02-07 | 郑州赛图康生物科技有限公司 | Reagent impurity precipitation device |
-
2021
- 2021-10-18 CN CN202111212214.1A patent/CN113943640A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110159533A1 (en) * | 2008-07-29 | 2011-06-30 | Bastien Karkouche | Bioparticle capture device, and use thereof |
| CN102165303A (en) * | 2008-07-29 | 2011-08-24 | 巴斯蒂安·卡尔库切 | Bioparticle capture device, and use thereof |
| CN206736238U (en) * | 2017-05-09 | 2017-12-12 | 周口师范学院 | A kind of cell filter |
| CN109731107A (en) * | 2019-01-26 | 2019-05-10 | 临海市科晶生物科技有限公司 | A kind of compound disposable high-efficiency quick liquid sterilization purification device and its application method |
| CN210021758U (en) * | 2019-05-05 | 2020-02-07 | 郑州赛图康生物科技有限公司 | Reagent impurity precipitation device |
Non-Patent Citations (1)
| Title |
|---|
| 龚雪平等: "粉尘捕集用滤膜的性能实验结果", 中国环境监测, vol. 12, no. 03, pages 36 - 38 * |
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| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220118 |