CN113933089B - Extraction device for phloem micro-tissue of citrus yellow dragon disease tree body - Google Patents
Extraction device for phloem micro-tissue of citrus yellow dragon disease tree body Download PDFInfo
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- CN113933089B CN113933089B CN202111206321.3A CN202111206321A CN113933089B CN 113933089 B CN113933089 B CN 113933089B CN 202111206321 A CN202111206321 A CN 202111206321A CN 113933089 B CN113933089 B CN 113933089B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/02—Devices for withdrawing samples
- G01N1/04—Devices for withdrawing samples in the solid state, e.g. by cutting
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/42—Low-temperature sample treatment, e.g. cryofixation
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Abstract
The invention discloses a citrus yellow dragon disease tree phloem micro-tissue extraction device, which comprises a frame body, a storage area and an operation area, wherein the storage area is arranged at the bottom of the frame body, the storage area adopts a drawer type structure for pushing and pulling, a handle is arranged on a front panel of the storage area, the operation area is fixed at the upper part of the frame body, the operation area is divided into a plurality of groups of fixed operation compartments, a sample discharging hole is arranged at one side of the operation compartment close to the handle, a replaceable sampling tube is embedded into one side of the operation compartment far away from the handle, a threaded hole is arranged at the top of the left side of the sampling tube, a sampling needle head is arranged at the right side of the sampling tube, the sampling tube is controlled to be sampled by an operation rod or enter the storage area, and the drawer is provided with a plurality of groups of storage compartments, so that samples obtained by different operation compartments can be respectively stored in corresponding storage compartments, and cross contact of the samples is prevented. The device has the advantages of simplicity, convenience and high efficiency, and can be used for tissue trace extraction and real-time preservation.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a plant phloem micro-tissue extraction device in the technical field of biology.
Background
Citrus yellow dragon disease is a destructive disease in the citrus industry and is widely distributed in main areas of citrus such as asia, africa, america and the like, caused by phloem-restricted bacteria (Candiatus Liberibacter) which cannot be artificially cultured so far, and is transmitted in the field by taking diaphorina citri as the only natural transmission medium. At present, no disease-resistant variety is available in production, and once the tree body is infected with diseases, the disease cannot be cured, but can be cut off, and the destructive hazard brought by the disease to the citrus industry is known as cancer of the citrus industry.
In order to enhance the monitoring, prevention and control of citrus yellow dragon disease in production, a large number of branches of citrus plants in an orchard are collected and sent to a laboratory for DNA extraction and yellow dragon disease detection. At present, the branches collected manually are bigger and easy to cause damage to the tree body, and inconvenient and rapid sampling is carried out in a large quantity, meanwhile, the collected branches are easy to be influenced by the preservation temperature or pollution of other factors in the storage and transportation process, so that the collected branches are withered, rotten and damaged, the accuracy of the subsequent yellow dragon disease detection is difficult to ensure, and therefore, the device for extracting the phloem trace tissue of the citrus yellow dragon disease tree body is necessary to be developed, and the continuous trace sampling and real-time low-temperature preservation of the collected samples are realized.
Disclosure of Invention
The invention aims to provide a tree phloem microsampling device which has continuous sampling capability and real-time low-temperature storage, and samples are not crossed, so that the device can be used in the fields of phloem microsampling of a yellow dragon disease tree and the like.
In order to achieve the above purpose, the technical scheme adopted by the invention is as follows: the invention relates to a microsampling device, which comprises a frame body, a storage area and an operation area, and is characterized in that: the frame body is of a cuboid structure, the storage area is arranged at the bottom of the frame body, and the operation area is fixed in the upper space of the frame body; the storage area is in a drawer-type structure push-pull design, and a handle is arranged on the front panel of the storage area; the operation area is divided into a plurality of groups of fixed operation compartments, each operation compartment is formed by closing two baffles on the left side and the right side and the front side and the rear side of the frame body to form an operation space, each operation compartment can be independently operated, the baffles are embedded into and fixedly connected to the front side and the rear side of the frame body, the operation compartment is far away from the handle side to be embedded into the replaceable sampling tube, a bottom plate is fixedly arranged at the bottom of the operation compartment and used for supporting the sampling tube and isolating the operation area from the storage area, a section of guide rail is arranged on the side face of the baffles on the two sides of the operation compartment, the sampling tube is mounted on the guide rail, the sampling tube can freely slide back and forth on the guide rail, a sampling hole is formed on one side of the bottom plate close to the handle, a sampling hole is formed under the sampling hole, a cover plate is arranged on the front side of the bottom plate, the cover plate can be embedded into the baffle of the operation area in a mode of being capable of being embedded into the mode of grooves and the like, the cover plate can be stirred down and then be pushed down to be just under the sampling hole, and can be separated from the storage area when the sampling hole is pushed down by a screwdriver, and the sample can be just pushed down to be exposed from the guide rail to be pushed down to be inserted into the storage area.
The utility model provides a tree phloem trace sampling device which characterized in that: the storage area is designed to be a push-pull drawer which is arranged at the bottom of the frame body, so that samples can be conveniently taken out in batches, the drawer is divided into a front part and a rear part, a plurality of groups of storage compartments are arranged at the front end, one group of storage compartments corresponds to one group of operation compartments right above, samples obtained from different operation compartments can be respectively stored in the corresponding storage compartments, cross contact of the samples is prevented, sampling staff can conveniently sample continuously, and samples from different sources can be stored; the rear part of the drawer is a cooling area, and the cooling area is filled with ice cubes or liquid nitrogen to realize low-temperature preservation of the sample, so that the drawer can be used for rapidly freezing biological tissues and preventing the biological tissues from being damaged.
The top of one side of the sampling tube is provided with a threaded hole, the other side of the sampling tube is provided with a sampling needle head shape, the sampling tube is controlled to sample or enter a storage area by an operating rod, the bottom of the operating rod is provided with external threads, the operating rod is connected to the sampling tube by being screwed into the threaded hole in a butt joint way through the external threads, thereby pushing and pulling the sampling tube to move back and forth on a guide rail through the operating rod, when the operating rod is pushed and pulled to the upper part of the sample discharging hole, the operating rod and the sampling tube fall into the corresponding storage compartment, the external thread is unscrewed to take out the operating rod, then the sampling tube is stored in the storage compartment, and the storage compartment can be sealed by stirring the cover plate to the lower part of the sample discharging hole.
The sampling needle head of the sampling tube can be in the shape of a conical knife edge, a bevel knife edge or an arc cutting knife edge and the like, so that a proper sampling needle head can be conveniently selected for trees with different hardness and thickness, and scale marks are arranged on the sampling needle head, so that the quantification of the sampling depth of a target tree body can be realized.
Before sampling, a cooling area of the drawer is filled with a cooling agent such as ice cubes or liquid nitrogen, the drawer is filled into a storage area at the bottom of the frame body, the cover plate is shifted to seal the sampling hole so as to prevent the storage area from being polluted, the sampling tube is filled into the operation compartment, and the operation rod is connected to the sampling tube through external threads.
During sampling, the operating rod is pushed and pulled to enable the sampling tube to move forwards along the guide rail until the sampling needle exposes out of the frame body, the operating rod is pushed continuously to enable the sampling needle to be pricked into phloem of the tree body to sample, and after sampling is completed, the operating rod is pulled backwards to enable the sampling tube to leave the tree body.
When a sample is kept, the cover plate is stirred firstly as shown in the figure, the lower sample hole is ensured to be exposed, the operating rod is pulled back to the upper part of the lower sample hole, the external thread is unscrewed to take out the operating rod, then a sampling tube carrying the sample falls into a corresponding storage compartment for storage, and the cover plate is stirred to cover the lower sample hole so as to close the corresponding storage compartment. Repeating the above operation in the next operation compartment can complete the next sampling and store the sample in the corresponding storage compartment, thereby realizing the on-site continuous sampling.
When the sampled sample is needed, the sampling tube containing the sample can be taken out only by pulling out the drawer, the sampling tube can be mounted in the operation compartment again by cleaning the sampling tube after the sample is used up, a new sampling tube can be replaced in the operation compartment, and the next batch of sampling can be performed by repeating the steps.
The phloem micro-tissue sampling device has the advantages of simplicity in operation, high efficiency, good sample preservation, small sampling amount and the like, so that the phloem micro-tissue sampling device has the advantages of high efficiency, wide applicability and small sampling amount, and achieves the effect of avoiding high-temperature necrosis.
Drawings
FIG. 1 is a schematic diagram of a micro-tissue sampling device.
FIG. 2 is a schematic diagram of the operation compartment of the micro-tissue sampling device.
Fig. 3 is a schematic view of a baffle structure at two sides of the operation compartment.
Fig. 4 is a schematic view of the structure of the bottom plate of the operation compartment.
Fig. 5 is a schematic view of a drawer structure in a storage area.
FIG. 6 is a cross-sectional view of a sampling tube in a micro-tissue sampling device.
FIG. 7 is a cross-sectional view of a lever in a micro-tissue sampling device.
FIG. 8 is a schematic diagram of the operation of the micro-tissue sampling.
FIG. 9 is a schematic illustration of the storage of a sample tube in a storage area after sampling of a microstructure.
Detailed Description
The preferred embodiments of the present invention will be described in detail below with reference to the attached drawings: it should be understood that the preferred embodiments are presented by way of illustration only and not by way of limitation.
As shown in fig. 1, the invention relates to a tree phloem micro-tissue sampling device, which comprises a frame body 1, a storage area 2 and an operation area 3, and is characterized in that: the frame body 1 is of a cuboid structure, the storage area 2 is arranged at the bottom of the frame body 1, and the operation area 3 is fixed in the upper space of the frame body 1; the storage area 2 is in a drawer-type structure push-pull design, and a handle 4 is arranged on the front panel of the storage area 2; the operation area 3 is divided into a plurality of groups of fixed operation compartments 5 shown in fig. 2, each operation compartment 5 is formed by closing two baffle plates 5.1 shown in fig. 3 on the left side and the right side and two sides of the frame body 1 to form an operation space, one side of the operation compartment 5 far away from the handle 4 is opened so as to facilitate sampling operation, each operation compartment can independently implement operation, the baffle plates 5.1 are embedded and fixedly connected onto the front surface and the rear surface of the frame body 1, the operation compartments 5 are far away from one side of the handle 4 and are embedded with a replaceable sampling tube 6, a bottom plate 5.2 shown in fig. 3 is fixedly arranged at the bottom of the operation compartment 5 and is used for isolating the operation area from the storage area, a section of guide rail 5.3 is arranged on the side of the baffle plates on the two sides of the operation compartment 5, the sampling tube 6 is arranged on the guide rail 5.3, the bottom plate 5.2 is close to one side of the handle 4 and is provided with a lower sample hole 5.4, the lower surface of the sample hole 5.5.5 is arranged under the sample hole 5.5, the sample hole can be opened when the sample hole is just below the handle 5.5.5 is in front of the cover plate 5.5.5, the sample hole is opened when the sample hole is opened by the screw driver, the sample hole is opened under the cover plate 5.5.5.5 is just in front of the cover plate 5.4, and the sample hole is opened by the cover plate 5.5, and the sample hole is just under the sample hole is opened by the cover plate 5.5, and the sample hole is just arranged under the sample hole is just can be opened by the cover plate 5, and the sample hole is just can be opened by the sample hole is just is opened by the sample hole is opened.
The utility model provides a tree phloem trace sampling device which characterized in that: the storage area 2 is designed to be a push-pull drawer 7 as shown in fig. 5, which is arranged at the bottom of the frame body 1, so that samples can be conveniently taken out in batches, the drawer 7 is divided into a front part and a rear part, the front end is provided with a plurality of groups of storage compartments 7.1, one group of storage compartments corresponds to a group of operation compartments 5 above, samples obtained from different operation compartments can be respectively stored in the corresponding storage compartments, cross contact of the samples is prevented, continuous sampling by sampling staff is facilitated, and samples from different sources can be stored; the rear part of the drawer 7 is provided with a cooling area 7.2, and the cooling area 7.2 is filled with ice cubes or liquid nitrogen to realize low-temperature preservation of the sample, so that the sample can be used for rapidly freezing biological tissues and preventing the biological tissues from being damaged.
The top of one side of the sampling tube 6 is provided with a threaded hole 6.1 shown in fig. 6, the other side of the sampling tube 6 is provided with a sampling needle head shape, the sampling tube 6 is controlled to sample or enter a storage area by an operation rod 8 shown in fig. 7, the bottom of the operation rod 8 is provided with an external thread 8.1, the operation rod 8 is connected to the sampling tube 6 by being in butt joint with the threaded hole 6.1 through the external thread 8.1, so that the sampling tube 6 can be pushed and pulled on a guide rail to move back and forth through the operation rod 8, the operation rod 8 falls into a corresponding storage compartment 7.1 together with the sampling tube 6 when being pushed and pulled above a sample discharging hole 5.4, the operation rod 8.1 is unscrewed to take out the operation rod 8, the sampling tube 6 is stored in the storage compartment 7.1, and the storage compartment 7.1 can be sealed by stirring a cover plate 5.5 to the lower part of the sample discharging hole 5.4.
The sampling needle of the sampling tube 6 can be in the shape of a conical knife edge 6.1, a bevel knife edge 6.2 or an arc knife edge 6.3 and the like, so that a proper sampling needle can be conveniently selected for trees with different hardness and thickness, and scale marks are arranged on the sampling needle, thereby realizing the quantification of the sampling depth of a target tree.
Before sampling, a cooling area 7.2 of the drawer 7 is filled with a cooling agent such as ice cubes or liquid nitrogen, the drawer 7 is filled into the storage area 2 at the bottom of the frame body 1, the cover plate 5.5 is shifted to seal the sample discharging hole 5.4 so as not to pollute the storage area 2, the sampling tube 6 is filled into the operation compartment 5, and the operation rod 8 is connected to the sampling tube 6 through the external thread 8.1.
During sampling, as shown in fig. 8, the operating rod 8 is pushed and pulled to enable the sampling tube 6 to move forward along the guide rail until the sampling needle exposes out of the frame body 1, the sampling needle can be pricked into phloem of the tree body 9 to sample after the operating rod 8 is pushed continuously, and the operating rod 9 is pulled backwards after sampling is completed to enable the sampling tube 6 to leave the tree body 9.
When a sample is kept, as shown in fig. 9, the cover plate 5.5 is firstly shifted to ensure that the sample discharging hole 5.4 is exposed, the operating rod 8 falls into the corresponding storage compartment 7.1 together with the sampling tube 6 when being pushed and pulled above the sample discharging hole 5.4, the external thread 8.1 is unscrewed to take out the operating rod 8, then the sampling tube 6 is stored into the storage compartment 7.1, and the cover plate 5.5 is shifted to cover the sample discharging hole 5.4 so as to close the corresponding storage compartment 7.1. Repeating the above operation in the next operation compartment can complete the next sampling and store the sample in the corresponding storage compartment, thereby realizing the on-site continuous sampling.
When the sampled sample is needed, the sampling tube containing the sample can be taken out only by drawing the drawer 7, the sampling tube can be mounted on the operation compartment 5 again by cleaning the sampling tube after the sample is used up, or a new sampling tube can be replaced to the operation compartment 5, and the next batch of sampling can be performed by repeating the steps.
Although embodiments of the present invention have been described in connection with the accompanying drawings, various modifications and variations may be made by those skilled in the art without departing from the spirit and scope of the invention, and such modifications and variations fall within the scope of the invention as defined by the appended claims.
Claims (7)
1. The utility model provides a microsampling device, includes framework, storage area and operation district, its characterized in that: the storage area is arranged at the bottom of the frame body, the operation area is fixed at the upper space of the frame body, the operation area is divided into a plurality of groups of fixed operation compartments, each operation compartment is formed by closing two baffle plates on the left side and the right side and the front side and the rear side of the frame body, each operation compartment can be independently operated, the baffle plates are embedded and fixedly connected to the front surface and the rear surface of the frame body, a handle is arranged on the front panel of the storage area, the operation compartment is far away from a removable sampling tube, a threaded hole is arranged at the top of one side of the sampling tube, the other side of the sampling tube is in the shape of a sampling needle head, the sampling tube is controlled by an operation rod and stored in the storage area, an external thread is arranged at the bottom of the operation rod, the operation rod is connected to the sampling tube through butt joint of the threaded hole, a section of guide rail is arranged on the side surface of the baffle plates on the two sides of the operation compartment, the sampling tube can freely slide on the front surface and the rear surface of the guide rail, the sampling tube can conveniently slide in the front surface of the guide rail, the sampling tube can move in the drawer and can conveniently enter the storage area under the drawer and the bottom of the storage area, the drawer is arranged at the bottom of the storage area, the storage area is in the drawer is in the position of the drawer, and the storage area is in the position of the drawer.
2. The microsampling device of claim 1, wherein: the method is applied to microsampling of phloem of the tree body.
3. The microsampling device of claim 2, wherein: the sampling needle head of the sampling tube is a conical knife edge, a bevel knife edge or an arc cutting knife edge, so that the proper sampling needle head can be conveniently selected for trees with different hardness and thickness.
4. The microsampling device of claim 1, wherein: the sample discharging hole is provided with a cover plate, the cover plate is arranged below the bottom plate, the cover plate is embedded into the baffle plate of the operation area in an embedded groove mode, the cover plate can be shifted back and forth below the bottom plate, the cover plate is shifted to just seal the sample discharging hole when being shifted to the lower part of the sample discharging hole, the cover plate is shifted to expose the sample discharging hole when the sample is to be stored, and the sampling tube is slid to the position right above the sample discharging hole and can enter the storage area after being separated from the guide rail.
5. The microsampling device of claim 1, wherein: one group of the storage compartments corresponds to one group of the operation compartments right above, and samples obtained from different operation compartments can be stored in the corresponding storage compartments respectively.
6. The microsampling device of claim 1, wherein: the cooling area is filled with ice cubes or liquid nitrogen to realize low-temperature preservation of the sample.
7. A sampling method using the microsampling device according to any one of claims 1-6, wherein during sampling, the sampling tube is moved forward until the sampling needle exposes the frame body for sampling, and the sampling tube is moved backward to leave after sampling is completed; when the sample is kept, the sample discharging hole is exposed, the sampling tube carrying the sample is moved backwards to the sample discharging hole, and the sampling tube falls into the corresponding storage compartment for storage.
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| CN202111206321.3A CN113933089B (en) | 2021-10-16 | 2021-10-16 | Extraction device for phloem micro-tissue of citrus yellow dragon disease tree body |
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| CN202111206321.3A CN113933089B (en) | 2021-10-16 | 2021-10-16 | Extraction device for phloem micro-tissue of citrus yellow dragon disease tree body |
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| CN113933089B true CN113933089B (en) | 2023-08-29 |
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| CN114958875B (en) * | 2022-05-15 | 2023-08-18 | 赣南师范大学 | Screening and application of reference gene metG of citrus yellow-long pathogen |
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| CN211767212U (en) * | 2020-02-23 | 2020-10-27 | 天津庆祥科技有限公司 | Portable scientific research sample bin |
| CN212638283U (en) * | 2020-03-07 | 2021-03-02 | 广东省农业科学院植物保护研究所 | Portable and efficient device for sampling field plant disease samples |
| CN212221103U (en) * | 2020-05-21 | 2020-12-25 | 广西民族师范学院 | Sampling device for field living tissue |
| CN212922565U (en) * | 2020-07-16 | 2021-04-09 | 全国农业技术推广服务中心 | Plant disease and insect pest leaf quarantine sample sampling box |
| CN212780044U (en) * | 2020-08-17 | 2021-03-23 | 河南大学 | Bark sampler |
| CN213036532U (en) * | 2020-08-20 | 2021-04-23 | 裕华生态环境股份有限公司 | Ecological remediation's urban wetland park plant's sample receiver |
| CN212852304U (en) * | 2020-09-17 | 2021-04-02 | 中华人民共和国日照海关 | Sample collection and preservation device for plant quarantine |
| CN214325647U (en) * | 2020-11-17 | 2021-10-01 | 海南大学 | Plant specimen collection box |
| CN213863354U (en) * | 2020-11-27 | 2021-08-03 | 辽宁生态工程职业学院 | Horticulture plant disease and pest sample collection box |
| CN214952224U (en) * | 2021-04-09 | 2021-11-30 | 新疆农垦科学院 | High-throughput sampling device for plant genome research |
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