CN113907044A - Breeding method and application of disease-resistant pig strain - Google Patents
Breeding method and application of disease-resistant pig strain Download PDFInfo
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- 201000010099 disease Diseases 0.000 title claims abstract description 102
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 102
- 238000009395 breeding Methods 0.000 title claims abstract description 33
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- 238000000034 method Methods 0.000 claims abstract description 24
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- 238000009396 hybridization Methods 0.000 claims abstract description 11
- 238000009402 cross-breeding Methods 0.000 claims abstract description 8
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- 230000009027 insemination Effects 0.000 claims description 10
- 230000004720 fertilization Effects 0.000 claims description 9
- 238000000338 in vitro Methods 0.000 claims description 9
- 241000272525 Anas platyrhynchos Species 0.000 claims description 8
- 210000002459 blastocyst Anatomy 0.000 claims description 8
- 206010012735 Diarrhoea Diseases 0.000 claims description 7
- 208000007212 Foot-and-Mouth Disease Diseases 0.000 claims description 6
- 241000710198 Foot-and-mouth disease virus Species 0.000 claims description 6
- 208000005577 Gastroenteritis Diseases 0.000 claims description 4
- 208000007407 African swine fever Diseases 0.000 claims description 3
- 208000001726 Classical Swine Fever Diseases 0.000 claims description 3
- 208000005342 Porcine Reproductive and Respiratory Syndrome Diseases 0.000 claims description 3
- 241000202347 Porcine circovirus Species 0.000 claims description 3
- 241000702619 Porcine parvovirus Species 0.000 claims description 3
- 241000702665 Porcine rotavirus Species 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 229920001184 polypeptide Polymers 0.000 claims description 3
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 3
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 3
- 229940124859 Rotavirus vaccine Drugs 0.000 claims description 2
- 208000035240 Disease Resistance Diseases 0.000 abstract description 11
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/027—New breeds of vertebrates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0604—Whole embryos; Culture medium therefor
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2207/00—Modified animals
- A01K2207/12—Animals modified by administration of exogenous cells
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/10—Mammal
- A01K2227/108—Swine
Abstract
The present invention belongs to the field of animal crossbreeding technology. In particular to a method for breeding disease-resistant pig strains. The method takes Duroc pigs as male parents and Japanese pagoda pigs as female parents for hybridization, and the offspring is F1 generation hybridized pigs; and crossing 8 generations of the F1 hybrid pig serving as a parent to obtain the disease-resistant pig KBZ 8. The results of the examples show that the disease-resistant pig KBZ8 obtained by the breeding method disclosed by the invention is good in growth performance and high in litter size, can resist pig diseases, particularly can effectively prevent pseudorabies and infection of epidemic abdomens, and the obtained disease-resistant pig KBZ8 can be used as a male parent to be hybridized with pigs of other breeds, so that the disease resistance of offspring is further improved.
Description
Technical Field
The present invention belongs to the field of animal crossbreeding technology. In particular to a method for breeding disease-resistant pig strains.
Background
The variety of the pig is very many, and according to data statistics, the local variety of the pig in China exceeds 50. With the continuous development of the pig industry, the breeds of the pigs are continuously updated and iterated, however, various pig diseases occur every year, and the pig diseases become important problems causing serious economic losses of farms. In particular, extremely serious problems arise if the disease progresses to postweaning multisystemic wasting syndrome characterized by neonatal piglet death, persistent atrophy and daily weight loss, or if the disease progresses to porcine respiratory syndrome. In order to avoid and reduce the economic loss caused by the infection of the pig diseases, a new strain of pig with good disease resistance is urgently needed.
Disclosure of Invention
The invention aims to provide a breeding method of a disease-resistant pig strain, which has the advantages of good pig growth performance, high litter size and good disease resistance.
In order to achieve the above purpose, the invention provides the following technical scheme:
the invention provides a method for breeding disease-resistant pigs with KBZ8, which comprises the following steps:
the Duroc pigs are taken as male parents, the pagoda pigs are taken as female parents for hybridization, and the offspring is F1 generation hybrid pigs;
and crossing 8 generations of the F1 hybrid pig serving as a parent to obtain the disease-resistant pig KBZ 8.
Preferably, the means of crossing comprises artificial insemination.
Preferably, the crossing comprises in vitro fertilization to obtain fertilized eggs.
Preferably, after obtaining the fertilized egg, the method further comprises the steps of placing the fertilized egg into a culture solution to be cultured until a blastocyst embryo is formed, and implanting the fertilized egg into the uterine wall of the female parent to obtain the offspring hybrid pig.
Preferably, 1mL of vaccine is included per 100mL of the culture medium.
Preferably, the vaccine comprises a polypeptide vaccine or a genetically engineered vaccine.
Preferably, the porcine disease comprises one or more of porcine parvovirus, porcine pseudorabies, porcine foot and mouth disease, porcine transmissible gastroenteritis, porcine rotavirus disease, porcine circovirus, classical and African swine fever, porcine epidemic diarrhea and porcine reproductive and respiratory syndrome.
Preferably, when the F1 hybrid pig is used as the female parent of the cross, the selection criteria for the male parent of the cross, F1 generation hybrid pig, include: the weight of the adult duck is more than or equal to 90kg after the adult duck is 11-13 months old; selection criteria for female parent F1 generation hybrid pigs for crossing included: the weight of the adult duck is more than or equal to 80kg after the adult duck is 11-13 months old.
The invention also provides application of the disease-resistant pig KBZ8 obtained by the breeding method in breeding disease-resistant pig strains.
The invention also provides a breeding method of the new disease-resistant pig strain, and the disease-resistant pig strain is obtained by taking the diseased pig KBZ8 obtained by the breeding method as a male parent and hybridizing the male parent with a breed pig.
The invention provides a breeding method of a disease-resistant pig strain, which comprises the following steps: the Duroc pigs are taken as male parents, the pagoda pigs are taken as female parents for hybridization, and the offspring is F1 generation hybrid pigs; and crossing 8 generations of the F1 hybrid pig serving as a parent to obtain the disease-resistant pig KBZ 8. The disease-resistant pig KBZ8 has good growth performance, high litter size and good disease resistance.
The disease-resistant pig KBZ8 bred by the invention can be used as a hybrid male parent to be hybridized with pigs of any variety, so that the disease-resistant pig strain with good growth performance, high litter size and better disease resistance is obtained.
Drawings
In order to more clearly illustrate the technical solution in the embodiments of the present invention, the drawings required to be used in the embodiments will be briefly described below.
FIG. 1 is a flow chart of a method for breeding disease-resistant pigs KBZ8 according to examples 1-4;
FIG. 2 is a flowchart of a method for breeding disease-resistant pigs according to the new strain of embodiments 6-7.
Detailed Description
The invention provides a breeding method of a disease-resistant pig strain, which comprises the following steps: the Duroc pigs are taken as male parents, the pagoda pigs are taken as female parents for hybridization, and the offspring is F1 generation hybrid pigs; and crossing 8 generations of the F1 hybrid pig serving as a parent to obtain the disease-resistant pig KBZ 8.
The method takes Duroc pigs as male parents and Japanese pagoda pigs as female parents for hybridization to obtain F1 generation hybrid pigs. The Duroc pigs have the capability of fast growth speed and robust body types, and the Sophora pigs have high reproductive performance capability. The Duroc pigs and the Sophora japonica pigs are purchased from Fujian Yongcheng farming and pasturing technology group Limited and Shang Hangzhou ao Sophora japonica pig industry development Limited respectively. The method of crossing according to the present invention is preferably artificial insemination, and the method of artificial insemination according to the present invention is not particularly limited, and may be performed by a method generally used in the art. The F1 generation hybrid pig parent has strong limbs and good growth performance.
After obtaining F1 generation hybrid pig, the invention takes the F1 generation hybrid pig as parent, and fixes 8 generations in a crossing way to obtain disease-resistant pig KBZ 8. When the F1 generation hybrid pig is used as a parent for crossbreeding, the invention preferably selects a male parent F1 generation hybrid pig for crossbreeding and a female parent F1 generation hybrid pig for crossbreeding according to characters, particularly, the male parent F1 generation hybrid pig for crossbreeding is 11-13 months old, and the weight is more than or equal to 90 kg; the female parent F1 generation hybrid pig for crossing is 11-13 months old and the weight is more than or equal to 80 kg. The transverse crossing mode of the invention is preferably in vitro fertilization, and more preferably, fertilized eggs obtained by the in vitro fertilization are placed in a culture solution to be cultured until blastocyst embryos are formed and are implanted into the uterine wall of a female parent to obtain offspring hybrid pigs. The method of in vitro fertilization according to the present invention is not particularly limited, and any conventional method in the art may be used.
In the present invention, 1mL of the vaccine is preferably included per 100mL of the culture solution. The vaccine of the present invention preferably comprises a polypeptide vaccine or a genetically engineered vaccine. The vaccine of the invention preferably comprises one or more of porcine parvovirus vaccine, porcine pseudorabies vaccine, porcine foot and mouth disease vaccine, porcine transmissible gastroenteritis vaccine, porcine rotavirus vaccine, porcine circovirus vaccine, classical swine fever and African swine fever vaccine, porcine epidemic diarrhea and porcine reproductive and respiratory syndrome vaccine. In the practice of the present invention, it is preferable that a corresponding vaccine is added to the culture solution according to the disease resistance of the desired target offspring hybrid, for example, 1mL of swine foot and mouth disease vaccine is added to 100mL of the culture solution for culturing fertilized eggs in order to make the offspring hybrid swine resistant to swine foot and mouth disease. The method for obtaining the offspring hybrid pig by in vitro fertilization can obtain the pig strain with good disease resistance and can deal with various pig diseases.
The method repeats the mode of obtaining offspring hybrid pigs by in vitro fertilization for 8 times to obtain disease-resistant pigs KBZ 8. So that the resistance of the disease-resistant pig KBZ8 to the pig diseases is stable. The obtained disease-resistant pig KBZ8 can be used as a hybridization parent to breed offspring hybridized pigs with higher disease resistance.
The invention also provides application of the disease-resistant pig KBZ8 obtained by the breeding method in breeding disease-resistant pig strains. The disease-resistant pig KBZ8 is preferably selected as a male parent and is hybridized with a breed pig to obtain a new strain disease-resistant pig. When the disease-resistant pig KBZ8 is taken as a male parent, the male parent disease-resistant pig KBZ8 for hybridization is preferably selected according to characters, specifically, the male parent disease-resistant pig KBZ8 for hybridization is 11-13 months old, and the weight is more than or equal to 90 kg. The breeding pigs are preferably white pigs and purchased from Fujian Yongcheng farming and pasturing science and technology group Limited. The method of crossing according to the present invention is preferably artificial insemination, and the method of artificial insemination according to the present invention is not particularly limited, and may be performed by a method generally used in the art. The disease-resistant pig strain obtained by the invention inherits the performance of the diseased pig KBZ8 and has stronger disease resistance.
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention.
Example 1
A method for breeding disease-resistant pig KBZ8 comprises the following steps:
(1) hybridizing Duroc pigs serving as male parents and Japanese pagoda pigs serving as female parents in an artificial insemination mode to obtain F1 generation hybridized pigs;
(2) selecting F1 generation adult boars (12 months old and 90kg in weight) and sows (12 months old and 80kg in weight) with good four limbs strong and growth performance, respectively and manually collecting sperm liquid and ova, and carrying out artificial in vitro fertilization;
(3) putting the fertilized eggs into 100mL of culture solution (the culture solution contains 1mL of porcine pseudorabies vaccine except nutrition required by the growth of the fertilized eggs) and culturing until blastocyst embryos are formed;
(4) then transplanting the blastocyst embryo into the uterine wall of the sow, and inoculating to obtain an F2 generation hybrid pig;
(5) and (4) repeating the steps (2) to (4), and obtaining the disease-resistant pig KBZ8 after artificial in vitro fertilization is carried out for 8 times (namely repeated for 7 times).
Example 2
The same as example 1 except that in step (3), the fertilized egg is placed in 100mL of a culture solution containing 1mL of porcine epidemic diarrhea vaccine in addition to nutrients required for the growth of the fertilized egg, and cultured until a blastocyst is formed.
Example 3
The difference from example 1 is that in step (3), the fertilized egg is placed in 100mL of culture medium (which contains 1mL of swine foot-and-mouth disease vaccine in addition to nutrients required for the fertilized egg to grow) and cultured until blastocyst is formed.
Example 4
The same as example 1 except that in step (3), the fertilized egg is placed in 100mL of a culture medium (which contains 1mL of transmissible gastroenteritis of swine vaccine in addition to nutrients required for the fertilized egg to grow) and cultured until a blastocyst is formed.
Example 6
The disease-resistant pig KBZ8 obtained in the example 1 is used as a male parent, a big white pig is used as a female parent, and an artificial insemination mode is adopted for hybridization to obtain the disease-resistant pig strain.
Example 7
The disease-resistant pig KBZ8 obtained in the example 2 is used as a male parent, a big white pig is used as a female parent, and an artificial insemination mode is adopted for hybridization to obtain the disease-resistant pig strain.
Test example 1
(1) Farrowing test
The test is divided into 5 groups, namely a disease-resistant pig KBZ8 group in example 1, a disease-resistant pig strain group in example 6, a Duroc pig group, a Japanese pagoda pig group and a big white pig group, wherein each group comprises 1 boar and 5 oestrus sows, the oestrus sows are bred in an artificial collection and artificial insemination mode, the same feeding mode is adopted for feeding to delivery, the number of piglets in each group is counted, and the statistical result is shown in the following table 1.
TABLE 1 farrowing results for different pig breeds
As can be seen from Table 1, the disease-resistant pigs obtained by the breeding method of the present invention have an average litter size of KBZ8 of 15 pigs, which is higher than 10 pigs of the Duroc group. The average litter size of the disease-resistant pig strain of the filial generation of the disease-resistant pig KBZ8 and the large white pig is 16, which is higher than 10 of Duroc group and 14 of large white pig group, and inherits the characteristic of high reproductive performance of Sophora japonica pigs. The disease-resistant pig strain obtained by the embodiment of the invention has high farrowing rate.
(2) Growth Performance test
The experiment is divided into 5 groups, each group of 3 weaned pigs with 3-4 weeks old is respectively a disease-resistant KBZ8 group of the disease-resistant pig of example 1, a disease-resistant pig strain group of example 6, a Duroc pig group, a Huai pig group and a large white pig group, each pig of each group is fed with complete feed (the feed is purchased from general North agricultural agriculture and animal husbandry Co., Ltd., Fuzhou) for 2 times every day, the pigs of each group are fed with the complete feed once in the morning and at night, the pigs of each group are continuously fed for 2 months, the weight conditions of the pigs of each group are counted, and the statistical results are shown in the following table 2.
TABLE 2 growth Performance of different pig lines
As can be seen from Table 2, the disease-resistant pig KBZ8 obtained by the breeding method of the invention inherits the characteristics of Duroc pigs, and the average weight of the disease-resistant pig KBZ8 after 2 months of breeding is 24.5kg, which is more than 22.1kg of the Japanese pagoda pig group. The average weight of the disease-resistant pig KBZ8 and the disease-resistant pig breed of the filial generation of the big white pig after being fed for 2 months is 26.4kg, which is more than 22.1kg of the pagoda pig group and more than 25.6kg of the big white pig group. The disease-resistant pig strain obtained by the embodiment of the invention has good growth performance.
(3) Porcine pseudorabies virus challenge protection test
The test is divided into 4 groups, each group having 4 young animals of 3-4 weeks oldThe pigs are a disease-resistant pig KBZ8 group (marked as a test group 1), a disease-resistant pig strain group (marked as a test group 2), a Japanese pagoda pig group (marked as a test group 3) and a white pig group (marked as a control group) in example 1, and 1mL of porcine pseudorabies virus (containing 10 percent of porcine pseudorabies virus) is orally taken by 1-3 pigs in the test groups respectively6TCID50) Control pigs were orally administered 1mL of saline. The disease of each pig was observed for 7 consecutive days, and the results are shown in Table 3.
TABLE 3 Pseudorabies virus challenge protection test results for different pig strains
| Group of | Test group 1 | Test group 2 | Test group 3 | Control group |
| Onset of disease | Onset of 0 disease | Onset of 0 disease | 4/4 onset of disease | Onset of 0 disease |
As can be seen from table 3, none of the disease-resistant pig lines of the disease-resistant pigs KBZ8 and KBZ8 obtained by the breeding method of the present invention and the disease-resistant pig lines of the filial generations of white pigs and the white pig group developed disease, and the pagoda pig group developed all diseases, which indicates that the disease-resistant pig lines obtained by the embodiments of the present invention have good disease resistance to porcine pseudorabies, and can effectively prevent infection of porcine pseudorabies.
(4) Porcine epidemic diarrhea toxicity attacking protection test
The test is divided into 4 groups, each group of 4 piglets with 3-4 weeks of age is respectively a disease-resistant KBZ8 group (marked as test group 1) of the disease-resistant pig in example 2, a disease-resistant pig strain group (marked as test group 2) in example 7, a pagoda pig group (marked as test group 3) and a large white pig group (marked as control group), and 1mL of epidemic diarrhea (containing 10 percent) is orally taken by 1-3 pigs in the test groups respectively6TCID50) Control pigs were orally administered 1mL of saline. The disease status of each pig was observed for 7 consecutive days, and the results are shown in Table 4.
TABLE 4 epidemic diarrhea attacking protection test results for different pig strains
| Group of | Test group 1 | Test group 2 | Test group 3 | Control group |
| Onset of disease | Onset of 0 disease | Onset of 0 disease | 4/4 onset of disease | Onset of 0 disease |
As can be seen from table 4, none of the disease-resistant pig KBZ8, the disease-resistant pig KBZ8, the disease-resistant pig line of the filial generation of the big white pig and the big white pig group, and the pagoda pig group all developed diseases, which are obtained by the breeding method of the present invention, indicate that the disease-resistant pig line obtained by the embodiment of the present invention has good disease resistance to the epidemic abdomen, and can effectively prevent the infection of the epidemic abdominal virus.
The embodiments show that the disease-resistant pig strain obtained by the breeding method provided by the invention has good growth performance and high litter size, can resist pig diseases, and particularly can effectively prevent the infection of porcine pseudorabies and epidemic abdomen.
Although the present invention has been described in detail with reference to the above embodiments, it is only a part of the embodiments of the present invention, not all of the embodiments, and other embodiments can be obtained without inventive step according to the embodiments, and the embodiments are within the scope of the present invention.
Claims (10)
1. The breeding method of the disease-resistant pig KBZ8 is characterized by comprising the following steps:
the Duroc pigs are taken as male parents, the pagoda pigs are taken as female parents for hybridization, and the offspring is F1 generation hybrid pigs;
and crossing 8 generations of the F1 hybrid pig serving as a parent to obtain the disease-resistant pig KBZ 8.
2. A method of breeding as claimed in claim 1, characterised in that the means of crossing comprises artificial insemination.
3. The method of claim 1, wherein the crossing comprises in vitro fertilization to obtain a fertilized egg.
4. The method as claimed in claim 3, wherein the step of obtaining the fertilized egg further comprises the step of culturing the fertilized egg in a culture solution until a blastocyst is formed, and implanting the fertilized egg into the uterine wall of the female parent to obtain the offspring hybrid pig.
5. A culture process according to claim 4, wherein 1mL of vaccine is included per 100mL of the culture.
6. The method of claim 5, wherein the vaccine comprises a polypeptide vaccine or a genetically engineered vaccine.
7. A method of breeding as claimed in claim 5, wherein the vaccine includes one or more of porcine parvovirus vaccine, porcine pseudorabies vaccine, porcine foot and mouth disease vaccine, porcine transmissible gastroenteritis vaccine, porcine rotavirus vaccine, porcine circovirus vaccine, classical and African swine fever vaccine, porcine epidemic diarrhea vaccine and porcine reproductive and respiratory syndrome vaccine.
8. The breeding method as claimed in claim 1, wherein when the F1 hybrid pig is used as female parent of crossbreeding, the selection criteria of male parent F1 generation hybrid pig for crossbreeding include: the weight of the adult duck is more than or equal to 90kg after the adult duck is 11-13 months old; selection criteria for female parent F1 generation hybrid pigs for crossing included: the weight of the adult duck is more than or equal to 80kg after the adult duck is 11-13 months old.
9. The application of the disease-resistant pig KBZ8 obtained by the breeding method of claim 1 in breeding disease-resistant pig strains.
10. A breeding method of a new disease-resistant pig strain is characterized in that the disease-resistant pig KBZ8 obtained by the breeding method of any one of claims 1 to 8 is used as a male parent and is hybridized with a breed pig to obtain the disease-resistant pig strain.
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| CN106884006A (en) * | 2015-12-16 | 2017-06-23 | 陕西溯源农业发展有限公司 | A kind of recombined adhenovirus and its construction method |
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