CN113906861A - Low-temperature stratification germination accelerating method for Cotoneaster multiforus seeds - Google Patents
Low-temperature stratification germination accelerating method for Cotoneaster multiforus seeds Download PDFInfo
- Publication number
- CN113906861A CN113906861A CN202111390533.1A CN202111390533A CN113906861A CN 113906861 A CN113906861 A CN 113906861A CN 202111390533 A CN202111390533 A CN 202111390533A CN 113906861 A CN113906861 A CN 113906861A
- Authority
- CN
- China
- Prior art keywords
- seeds
- cotoneaster
- temperature
- multiforus
- river sand
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/08—Immunising seed
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Soil Sciences (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Physiology (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention provides a low-temperature stratification method for Cotoneaster multiforus seeds, which comprises the following steps: adding hot water with the temperature of 100 ℃ into the prepared Cotoneaster multiforus seeds while stirring, soaking the seeds for 24 hours after uniformly stirring, then pouring out the soaked water, repeating the steps again, then soaking and disinfecting the seeds for 2 hours by using a potassium permanganate solution, and finally washing the seeds to be colorless by using clear water to obtain the treated seeds; and (2) filling the treated seeds into a mesh bag, layering the mesh bag filled with the treated seeds and the disinfected wet river sand, then carrying out sand storage for 120 days at the temperature of 2 ℃, then carrying out frozen storage for 30 days at the temperature of-15 ℃, then carrying out standing for 30 days at the temperature of 15 ℃, finally carrying out standing for 150 days at the temperature of 25 ℃, and completing the pregermination of Cotoneaster congous seeds. The invention can obviously improve the germination rate of Cotoneaster multiforus seeds through the steps of twice seed scalding, temperature changing and sand storage and the like.
Description
Technical Field
The invention belongs to the technical field of seed germination acceleration, and particularly relates to a low-temperature stratification method for Cotoneaster multiforus seeds.
Background
Cotoneaster multiflorus (the name of Cotoneaster ambiguus Rehd. et Wils.) is a plant of Cotoneaster multiflorus in the family of Rosaceae, deciduous shrub, up to 2 meters; the branches are bent, small branches are thin and thin, grey brown, and when young, the branches are rough and rough, and fall off or nearly have no hair after a while, and the branches are special plants in China; the blade is oval to rhombus oval, the length is 2.5-6 cm, the width is 1.5-3 cm, the tip is tapered to sharp, the base is wide and wedge-shaped, the edge is full, the upper part is young and tender and has hair thinning and softening, the lower part is tender and has hair thinning and softening in old time; the leaf stalk is 2-5 mm long and has hair; the leaves are in the shape of needles, most of which fall off and have sparse and soft hairs. The polystictus has 5-10 flowers, and the total peduncles and peduncles are thinned and fluffed; the bract is in the shape of a needle, slightly soft and early falls; the length of the flower stalk is 4-5 mm; calyx-shaped, without hair or with hair on the outside, and without hair on the inside; the sepals are triangular, the tips of the sepals are sharp, the outer surfaces of the sepals have no hairs or have gentle hairs only along the edges, and the inner surfaces of the sepals have no hairs; the petals are upright, wide, oval or nearly circular, the length and the width are respectively about 3-4 mm, the tip is round and blunt, the base is provided with short claws, and the white color is pink; stamen 20, slightly shorter than petals; 2-5 of flower pillar, which is separated from the flower pillar and is slightly shorter than the stamen; the distal end of the ovary is dense and soft. The fruit is oval or nearly spherical, 8-10 mm long, 6-7 mm in diameter, black, with a fine hair at the tip, and often 2-3(4-5) small cores. The flowering period is 5-6 months, and the fruiting period is 9-10 months, and the flowers are distributed in Shaanxi, Gansu, Sichuan, Guizhou, Yunnan, Ningxia and other places, and grow in mountainous regions, semi-sunny slopes and sparse forests with elevation of 1800 plus 2900 meters.
The traditional Chinese Cotoneaster multiflorus seed germination method has low germination rate and long seed dormancy period (the seed can be harvested and directly sowed in the current year and can emerge in the 3 rd year), so that the development of the Chinese Cotoneaster multiflorus seed germination method with high germination rate is needed.
Disclosure of Invention
The invention aims to solve the technical problem of providing a low-temperature stratification germination accelerating method for Cotoneaster multiflorus seed, which aims at overcoming the defects of the prior art.
In order to solve the technical problems, the invention adopts the technical scheme that: a low-temperature stratification germination accelerating method for Cotoneaster multiforus seeds comprises the following steps:
s1, adding hot water with the temperature of 100 ℃ into the prepared Cotoneaster multiforus seeds while stirring, soaking the seeds for 24 hours after uniformly stirring, pouring out the water for soaking the seeds, adding the hot water with the temperature of 100 ℃ into the seeds, uniformly stirring, pouring out the water for soaking the seeds for 24 hours, soaking and disinfecting the seeds for 2 hours by using a potassium permanganate solution, and finally washing the seeds with clear water until the seeds are colorless to obtain the treated seeds;
s2, filling the processed seeds obtained in the step S1 into mesh bags, placing the mesh bags filled with the processed seeds and the disinfected wet river sand in a layered mode, then placing the mesh bags at the temperature of 2 ℃ for 120 days, then transferring to the temperature of-15 ℃ for freezing and storing for 30 days, then transferring to the temperature of 15 ℃ for placing for 30 days, and finally transferring to the temperature of 25 ℃ for placing for 150 days, so that the accelerating germination of the Cotoneaster aster seeds is completed.
Preferably, the method for modulating the modulated Cotoneaster toneaster seed in S1 comprises the following steps: treading mature Cotoneaster multiforus fruits, putting the Cotoneaster multiforus fruits into a sieve with the aperture of 0.30-0.50 cm, repeatedly washing the Cotoneaster multiforus fruits under flowing clean water, pouring a sieved jam and seed mixture into a fine sieve with the aperture of 0.15-0.20 cm for repeatedly washing, placing the rest mixture in the fine sieve into a ventilating and drying place for drying in the shade, repeatedly rubbing the mixture, and removing impurities to obtain the modulated Cotoneaster multiforus seeds.
Preferably, the mass fraction of potassium permanganate in the potassium permanganate solution in S1 is 0.2%.
Preferably, the specification of the mesh bag in S2 is 20cm × 15cm, and the aperture is 0.2 cm.
Preferably, in S2, the mesh bag containing the treated seeds and the sterilized wet river sand are layered to form 3 layers, the lowermost layer is the sterilized wet river sand layer, the thickness of the sterilized wet river sand layer at the lowermost layer is 5cm, the thickness of the seed layer at the middle layer is 1cm, and the thickness of the sterilized wet river sand layer at the uppermost layer is 2 cm.
Preferably, the disinfected wet river sand is prepared by uniformly mixing 50% carbendazim wettable powder and the wet river sand, wherein the dosage of the 50% carbendazim wettable powder is 40g/m3。
Preferably, the humidity of the disinfected wet river sand is 55-60%.
Preferably, the frequency of spraying water to the sterilized wet river sand during the standing at the temperature in S2 is 1 time/d.
Preferably, the sowing environment of the Cotoneaster multiforus seeds subjected to pregermination in the S2 is a greenhouse, and the temperature of the greenhouse is-10 ℃ to 28 ℃.
Compared with the prior art, the invention has the following advantages:
1. the invention adopts hot water with the temperature of 100 ℃ to scald seeds twice, because the Cotoneaster multiforus has thin outer pericarp, fleshy mesocarp and hard and woody endocarp, is compact and hard, the seeds are difficult to break the endocarp to absorb moisture for germination, a certain amount of air can be left in the gap between the endocarp (namely seed shell) and the kernels after twice seed scalding, and the seeds are not easy to enter water after being soaked in cold water for a short time; the boiled water is adopted, because the air in the core is heated and expanded for a short time and overflows along the seam at the tail part of the core, when the water temperature is gradually cooled down, the seed shell is gradually cooled, the pressure in the core is reduced, water is sucked into the inside of the seed core, the seed kernels obtain sufficient moisture, and a foundation is laid for germination.
2. The invention adopts four-time temperature-changing sand storage, and the temperature-changing laminated sand storage method has the advantages of regular seedling emergence and high germination rate, and the four-time temperature change is realized by the following steps: under the condition of sand storage at the low temperature of 2 ℃ for 120 days for the first time, the solubility of oxygen is increased, the seed coat can be softened in a humid environment, and the permeability of the seed coat is increased; the seeds are frozen and stored for 30 days at the temperature of-15 ℃ for the second time, the respiration of the seeds can be reduced at low temperature, the seeds enter a transient dormancy state, and the storage life of the seeds is prolonged; standing at 15 deg.C for 30d to increase the solubility of oxygen and activity of enzyme, and softening seed coat to increase seed coat permeability; and the fourth time, the Cotoneaster multiforus is placed at the temperature of 25 ℃ for 150 days, the temperature is increased from the middle temperature environment to the high temperature environment, the activity of the enzyme is stronger, the seed coat is continuously softened, the permeability of the seed coat is increased, the embryo is obviously increased for the physiological after-ripening Cotoneaster multiforus seeds, the physiological after-ripening process is completed, and the germination rate and the germination vigor of the seeds are improved.
3. The germination accelerating method can improve the germination rate and the emergence rate of Cotoneaster multiforus seeds, and through a large number of experimental researches, the germination rate of twice scalding seeds is obviously higher than that of one time scalding seeds and more than two times scalding seeds, so that twice scalding seeds are selected.
The present invention will be described in further detail with reference to the accompanying drawings and examples.
Drawings
FIG. 1 is a graph showing the emergence of Cotoneaster multiforus seeds in example 1 of the present invention and in comparative examples 1 to 11.
FIG. 2 is a graph showing the emergence of Cotoneaster multiforus seed in comparative example 12.
FIG. 3 is a drawing showing the hot water blanching at 100 ℃ in example 1 of the present invention.
FIG. 4 is a graph of the seeds soaked with potassium permanganate in example 1 of the present invention.
FIG. 5 is a sand plot at a temperature of 2 ℃ in example 1 of the present invention.
FIG. 6 is a sand storage map at a temperature of 15 ℃ in example 1 of the present invention.
FIG. 7 is a sand storage map at a temperature of-15 ℃ in example 1 of the present invention.
Detailed Description
Example 1
The low-temperature stratification germination accelerating method for Cotoneaster acutifolius seeds comprises the following steps of:
firstly, preparing prepared Cotoneaster multiforus seeds: treading mature Cotoneaster multiforus fruits, putting the Cotoneaster multiforus fruits into a sieve with the aperture of 0.30cm, repeatedly washing the Cotoneaster multiforus fruits under flowing clean water, pouring a mixture of the sieved jam and the sieved seeds into a fine sieve with the aperture of 0.15cm for repeatedly washing, putting the remaining mixture in the fine sieve into a ventilating and drying place for drying in the shade, repeatedly rubbing the mixture, and removing impurities to obtain prepared Cotoneaster multiforus Cotoneaster seeds; the prepared seeds have no impurities, so that the subsequent seed germination acceleration is facilitated;
s1, adding hot water with the temperature of 100 ℃ into the prepared Cotoneaster multiforus seeds while stirring, soaking the seeds for 24 hours after uniformly stirring, pouring out the soaked water, adding the hot water with the temperature of 100 ℃ into the seeds and uniformly stirring, pouring out the soaked water after soaking for 24 hours, soaking and disinfecting the seeds for 2 hours by using a potassium permanganate solution with the mass fraction of 0.2%, and finally washing the seeds with clear water until the seeds are colorless to obtain the treated seeds;
s2, placing the processed seeds obtained in the step S1 in mesh bags with the specification of 20cm multiplied by 15cm and the aperture of 0.2cm, placing the mesh bags filled with the processed seeds and the disinfected wet river sand in 3 layers, wherein the lowermost layer is a disinfected wet river sand layer, the thickness of the lowermost layer is 5cm, the thickness of the middle layer is 1cm, the thickness of the uppermost layer is 2cm, placing the uppermost layer in a biochemical incubator at the temperature of 2 ℃ for 120 days, then transferring to a refrigerator at the temperature of-15 ℃ for freezing for 30 days, then transferring to a greenhouse at the temperature of 15 ℃ for placing for 30 days, finally transferring to a greenhouse at the temperature of 25 ℃ for placing for 150 days, and completing germination acceleration of the Cotoneaster aster Sichuan seeds;
the disinfected wet river sand is prepared by uniformly mixing 50% carbendazim wettable powder and the wet river sand, wherein the dosage of the 50% carbendazim wettable powder is 40g/m3The humidity of the disinfected wet river sand is 55%, and the wet river sand is kneaded into a mass by hand and released; the frequency of spraying water to the wet river sand during the variable temperature placement period is 1 time/d; the sowing environment of the Cotoneaster multiforus seeds which complete the pregermination in the S2 is a greenhouse, and the temperature of the greenhouse is 19 ℃.
Comparative example 1
The low-temperature stratification germination accelerating method of Cotoneaster multiflorus seed in the comparative example is the same as the method in the example 1, and the difference is that the water temperature for soaking the Cotoneaster multiflorus seed in the S1 is 80 ℃.
Comparative example 2
The low-temperature stratification germination accelerating method of Cotoneaster multiflorus seed in the comparative example is the same as the method in the example 1, and the difference is that the Cotoneaster multiflorus seed in the S1 is directly stored in sand without being disinfected by potassium permanganate after twice seed soaking.
Comparative example 3
The low-temperature stratification germination accelerating method of Cotoneaster multiflorus seed in the comparative example is the same as that in the example 1, except that the hot water with the temperature of 100 ℃ is adopted for soaking the seed in S1 for 24 hours.
Comparative example 4
The low-temperature stratification germination accelerating method of Cotoneaster multiflorus seed in the comparative example is the same as that in the example 1, except that the seed is soaked in hot water with the temperature of 100 ℃ for 48 hours in S1.
Comparative example 5
The low-temperature stratification germination accelerating method of Cotoneaster multiforus seeds in the comparative example is the same as that in the example 1, except that in S2, the mesh bag filled with the seeds and the disinfected wet river sand are placed in layers, the mesh bag and the disinfected wet river sand are placed at the temperature of 2 ℃ for 180 days, then the mesh bag and the disinfected wet river sand are frozen at the temperature of-15 ℃ for 60 days, then the mesh bag and the disinfected wet river sand are placed at the temperature of 15 ℃ for 60 days, and finally the mesh bag and the disinfected wet river sand are placed at the temperature of 25 ℃ for 30 days.
Comparative example 6
The low-temperature stratification germination accelerating method of Cotoneaster multiforus seeds in the comparative example is the same as that in the example 1, except that in S2, the mesh bag filled with the seeds and the disinfected wet river sand are placed in layers, the mesh bag and the disinfected wet river sand are placed at the temperature of 2 ℃ for 90 days, then the mesh bag and the disinfected wet river sand are frozen at the temperature of-15 ℃ for 90 days, then the mesh bag and the disinfected wet river sand are placed at the temperature of 15 ℃ for 120 days, and finally the mesh bag and the disinfected wet river sand are placed at the temperature of 25 ℃ for 30 days.
Comparative example 7
The low-temperature stratification germination accelerating method of Cotoneaster multiflorus seed in the comparative example is the same as the method in the example 1, and the difference is that the mesh bag filled with the seed and the disinfected wet river sand are placed in layers in S2 and are placed for 330 days at the temperature of 2 ℃.
Comparative example 8
The low-temperature stratification germination accelerating method of Cotoneaster multiflorus seed in the comparative example is the same as the method in the example 1, and the difference is that the mesh bag filled with the seed and the disinfected wet river sand are placed in layers in S2 and are placed for 330 days at the temperature of minus 15 ℃.
Comparative example 9
The low-temperature stratification germination accelerating method of Cotoneaster multiflorus seed in the comparative example is the same as the method in the example 1, and the difference is that the mesh bag filled with the seed and the disinfected wet river sand are placed in layers in S2 and are placed for 330 days at the temperature of 15 ℃.
Comparative example 10
The low-temperature stratification germination accelerating method of Cotoneaster multiflorus seed in the comparative example is the same as the method in the example 1, and the difference is that the mesh bag filled with the seed and the disinfected wet river sand are placed in layers in S2 and are placed for 330 days at the temperature of 25 ℃.
Comparative example 11
The low-temperature stratification germination method of Cotoneaster multiforus seed in the comparative example is the same as that in example 1, except that the treated seed obtained in S1 is not treated in the step S2.
Comparative example 12
The low-temperature stratification accelerating germination method of Cotoneaster multiflorus seed in the comparative example is the same as that in the example 1, but the difference is that the sowing environment of Cotoneaster multiflorus seed for completing accelerating germination in S2 is the field.
The seed collecting date, the sand storage date and the sowing date of the example 1 are the same as those of the comparative examples 1-12, and the germination and emergence conditions of Cotoneaster multiforus seeds under different treatment conditions are shown in Table 1.
TABLE 1 Cotoneaster multiforus seed germination and emergence under different treatment conditions
| Seeding environment | Average germination Rate (%) | Average rate of emergence (%) | |
| Example 1 | Greenhouse | 86.7 | 85.4 |
| Comparative example 1 | Greenhouse | 66.9 | 61.9 |
| Comparative example 2 | Greenhouse | 79.4 | 72.7 |
| Comparative example 3 | Greenhouse | 52.2 | 48.4 |
| Comparative example 4 | Greenhouse | 69.3 | 66.7 |
| Comparative example 5 | Greenhouse | 70.1 | 65.3 |
| Comparative example 6 | Greenhouse | 73.2 | 69.8 |
| Comparative example 7 | Greenhouse | 45.2 | 38.4 |
| Comparative example 8 | Greenhouse | 33.6 | 30.7 |
| Comparative example 9 | Greenhouse | 32.7 | 30.6 |
| Comparative example 10 | Greenhouse | 31.4 | 29.6 |
| Comparative example 11 | Greenhouse | 43.6 | 39.3 |
| Comparative example 12 | Field land | 57.1 | 52.2 |
The emergence under each treatment of greenhouse sowing is shown in fig. 1, and the emergence under field sowing is shown in fig. 2.
Example 2
The low-temperature stratification germination accelerating method for Cotoneaster acutifolius seeds comprises the following steps of:
firstly, preparing prepared Cotoneaster multiforus seeds: treading mature Cotoneaster multiforus fruits, putting the Cotoneaster multiforus fruits into a sieve with the aperture of 0.50cm, repeatedly washing the Cotoneaster multiforus fruits under flowing clear water, pouring a mixture of the sieved jam and the sieved seeds into a fine sieve with the aperture of 0.20cm for repeatedly washing, placing the remaining mixture in the fine sieve into a ventilating and drying place for drying in the shade, repeatedly rubbing the mixture, and removing impurities to obtain the prepared Cotoneaster multiforus seeds.
S1, adding hot water with the temperature of 100 ℃ into the prepared Cotoneaster multiforus seeds while stirring, soaking the seeds for 24 hours after uniformly stirring, then pouring out the soaked water, adding the hot water with the temperature of 100 ℃ into the seeds and uniformly stirring, then pouring out the soaked water after soaking for 24 hours, soaking and disinfecting the seeds for 2 hours by using a potassium permanganate solution with the mass fraction of 0.2%, and finally washing the seeds with clear water until the seeds are colorless to obtain the treated seeds;
s2, filling the processed seeds obtained in the step S1 into mesh bags with the specification of 20cm multiplied by 15cm and the aperture of 0.2cm, layering the mesh bags filled with the processed seeds and the disinfected wet river sand into 3 layers, wherein the lowermost layer is a disinfected wet river sand layer, the thickness of the lowermost disinfected wet river sand layer is 5cm, the thickness of the middle layer is 1cm, the thickness of the uppermost layer is 2cm, then placing the mesh bags at the temperature of 2 ℃ for 120 days, then freezing and storing the mesh bags at the temperature of-15 ℃ for 30 days, then placing the mesh bags at the temperature of 15 ℃ for 30 days, and finally placing the mesh bags at the temperature of 25 ℃ for 150 days to complete the germination acceleration of the Cotoneaster aster multiforus custarkii seeds.
The disinfected wet river sand is prepared by uniformly mixing 50% carbendazim wettable powder and the wet river sand, wherein the dosage of the 50% carbendazim wettable powder is 40g/m3The humidity of the disinfected wet river sand is 60%, and the wet river sand is kneaded into a ball by hand and released; the frequency of spraying water to the wet river sand during the variable temperature placement period is 1 time/d; the sowing environment of the Cotoneaster multiforus seeds which complete the pregermination in the S2 is a greenhouse, and the temperature of the greenhouse is-10 ℃.
The average germination rate of the Cotoneaster multiforus seeds subjected to pregermination in the embodiment is 57.0%, and the average emergence rate is 53.2%.
Example 3
The low-temperature stratification germination accelerating method for Cotoneaster acutifolius seeds comprises the following steps of:
firstly, preparing prepared Cotoneaster multiforus seeds: treading mature Cotoneaster multiforus fruits, putting the Cotoneaster multiforus fruits into a sieve with the aperture of 0.50cm, repeatedly washing the Cotoneaster multiforus fruits under flowing clear water, pouring a mixture of the sieved jam and the sieved seeds into a fine sieve with the aperture of 0.17cm for repeatedly washing, placing the remaining mixture in the fine sieve into a ventilating and drying place for drying in the shade, repeatedly rubbing the mixture, and removing impurities to obtain the prepared Cotoneaster multiforus seeds.
S1, adding hot water with the temperature of 100 ℃ into the prepared Cotoneaster multiforus seeds while stirring, soaking the seeds for 24 hours after uniformly stirring, then pouring out the soaked water, adding the hot water with the temperature of 100 ℃ into the seeds and uniformly stirring, then pouring out the soaked water after soaking for 24 hours, soaking and disinfecting the seeds for 2 hours by using a potassium permanganate solution with the mass fraction of 0.2%, and finally washing the seeds with clear water until the seeds are colorless to obtain the treated seeds;
s2, filling the processed seeds obtained in the step S1 into mesh bags with the specification of 20cm multiplied by 15cm and the aperture of 0.2cm, layering the mesh bags filled with the processed seeds and the disinfected wet river sand into 3 layers, wherein the lowermost layer is a disinfected wet river sand layer, the thickness of the lowermost disinfected wet river sand layer is 5cm, the thickness of the middle layer is 1cm, the thickness of the uppermost layer is 2cm, then placing the mesh bags at the temperature of 2 ℃ for 120 days, then freezing and storing the mesh bags at the temperature of-15 ℃ for 30 days, then placing the mesh bags at the temperature of 15 ℃ for 30 days, and finally placing the mesh bags at the temperature of 25 ℃ for 150 days to complete the germination acceleration of the Cotoneaster aster multiforus custarkii seeds.
The disinfected wet river sand is prepared by uniformly mixing 50% carbendazim wettable powder and the wet river sand, wherein the dosage of the 50% carbendazim wettable powder is 40g/m3The humidity of the disinfected wet river sand is 57%, and the wet river sand is kneaded into a mass by hands and released; the frequency of spraying water to the wet river sand during the variable temperature placement period is 1 time/d; the sowing environment of the Cotoneaster multiforus seeds which complete the pregermination in the S2 is a greenhouse, and the temperature of the greenhouse is 28 ℃.
The average germination rate of the Cotoneaster multiforus seeds subjected to pregermination in the embodiment is 57.3%, and the average emergence rate is 53.5%.
The above description is only for the preferred embodiment of the present invention, and is not intended to limit the present invention in any way. Any simple modification, change and equivalent changes of the above embodiments according to the technical essence of the invention are still within the protection scope of the technical solution of the invention.
Claims (9)
1. A low-temperature stratification germination accelerating method for Cotoneaster multiforus seeds is characterized by comprising the following steps:
s1, adding hot water with the temperature of 100 ℃ into the prepared Cotoneaster multiforus seeds while stirring, soaking the seeds for 24 hours after uniformly stirring, pouring out the water for soaking the seeds, adding the hot water with the temperature of 100 ℃ into the seeds, uniformly stirring, pouring out the water for soaking the seeds for 24 hours, soaking and disinfecting the seeds for 2 hours by using a potassium permanganate solution, and finally washing the seeds with clear water until the seeds are colorless to obtain the treated seeds;
s2, filling the processed seeds obtained in the step S1 into mesh bags, placing the mesh bags filled with the processed seeds and the disinfected wet river sand in a layered mode, then placing the mesh bags at the temperature of 2 ℃ for 120 days, then transferring to the temperature of-15 ℃ for freezing and storing for 30 days, then transferring to the temperature of 15 ℃ for placing for 30 days, and finally transferring to the temperature of 25 ℃ for placing for 150 days, so that the accelerating germination of the Cotoneaster aster seeds is completed.
2. The low-temperature stratification method for preparing Cotoneaster multiflorus seed, which is described in the step S1, is characterized in that the method for preparing the Cotoneaster multiflorus seed, which is prepared in the step S1, comprises the following steps: treading mature Cotoneaster multiforus fruits, putting the Cotoneaster multiforus fruits into a sieve with the aperture of 0.30-0.50 cm, repeatedly washing the Cotoneaster multiforus fruits under flowing clean water, pouring a sieved jam and seed mixture into a fine sieve with the aperture of 0.15-0.20 cm for repeatedly washing, placing the rest mixture in the fine sieve into a ventilating and drying place for drying in the shade, repeatedly rubbing the mixture, and removing impurities to obtain the modulated Cotoneaster multiforus seeds.
3. The low-temperature stratification method for Cotoneaster multiforus seeds as claimed in claim 1, wherein the mass fraction of potassium permanganate in the potassium permanganate solution in S1 is 0.2%.
4. The low-temperature stratification method for Cotoneaster multiforus seeds as claimed in claim 1, wherein the mesh bag in S2 has a size of 20cm x 15cm and a pore size of 0.2 cm.
5. The low-temperature stratification method for Cotoneaster multiforus seeds as claimed in claim 1, wherein the number of layers of the mesh bag filled with the treated seeds and the disinfected wet river sand in S2 is 3, the lowermost layer is the disinfected wet river sand layer, the thickness of the sterilized wet river sand layer of the lowermost layer is 5cm, the thickness of the seed layer of the middle layer is 1cm, and the thickness of the disinfected wet river sand layer of the uppermost layer is 2 cm.
6. The low-temperature stratification method of Cotoneaster multiforus seeds as claimed in claim 5, wherein the disinfected wet river sand is prepared by uniformly mixing 50% carbendazim wettable powder with wet river sand, and the dosage of the 50% carbendazim wettable powder is 40g/m3。
7. The low-temperature stratification method for Cotoneaster multiforus seeds as claimed in claim 6, wherein the humidity of the disinfected wet river sand is 55-60%.
8. The low-temperature stratification method of Cotoneaster multiforus seeds as claimed in claim 1, wherein the frequency of spraying water to the sterilized wet river sand during the warming and standing period in S2 is 1/d.
9. The low-temperature stratification method for Cotoneaster multiflorus seed according to claim 1, wherein the sowing environment of Cotoneaster multiflorus seed for completing germination in S2 is a greenhouse, and the temperature of the greenhouse is-10 ℃ to 28 ℃.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111390533.1A CN113906861A (en) | 2021-11-23 | 2021-11-23 | Low-temperature stratification germination accelerating method for Cotoneaster multiforus seeds |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111390533.1A CN113906861A (en) | 2021-11-23 | 2021-11-23 | Low-temperature stratification germination accelerating method for Cotoneaster multiforus seeds |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113906861A true CN113906861A (en) | 2022-01-11 |
Family
ID=79247613
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111390533.1A Pending CN113906861A (en) | 2021-11-23 | 2021-11-23 | Low-temperature stratification germination accelerating method for Cotoneaster multiforus seeds |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113906861A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116897776A (en) * | 2023-06-25 | 2023-10-20 | 神农架林区中医药产业协会 | Storage method, culture method and planting method of epimedium seeds |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1488239A (en) * | 2003-08-22 | 2004-04-14 | 中国科学院沈阳应用生态研究所 | Cotoneaster multiflorus grafting arbor method |
-
2021
- 2021-11-23 CN CN202111390533.1A patent/CN113906861A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1488239A (en) * | 2003-08-22 | 2004-04-14 | 中国科学院沈阳应用生态研究所 | Cotoneaster multiflorus grafting arbor method |
Non-Patent Citations (3)
| Title |
|---|
| 张小玲 等: "《种子学基础》", 31 May 2016 * |
| 盛玮 等: ""水栒子繁育技术对比研究"", 《辽宁林业科技》 * |
| 顾增辉 等: ""五种野生栒子种子的休眠与快速萌发"", 《植物生理学通讯》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116897776A (en) * | 2023-06-25 | 2023-10-20 | 神农架林区中医药产业协会 | Storage method, culture method and planting method of epimedium seeds |
| CN116897776B (en) * | 2023-06-25 | 2024-03-29 | 神农架林区中医药产业协会 | Storage method, culture method and planting method of epimedium seeds |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Percival | The wheat plant | |
| CN103703974B (en) | The cultivation method of a kind of sweet potato | |
| CN104380942B (en) | A kind of processing method making long of Rhizoma Paridis seed four months, coming into leaves for eight months | |
| CN102640639A (en) | Method for breeding seed konjac from konjac corms by removing terminal buds | |
| CN106069458B (en) | A kind of method for culturing seedlings of shaddock | |
| CN103975849A (en) | Breeding and cultivating method for early-matured, disease-resistant and loose purple cauliflowers | |
| CN106171755A (en) | A kind of implantation methods of four seasons Fructus Artocarpi Heterophylli | |
| CN106358929A (en) | Cultivation method for prolonging chicken-beak litchi picking period | |
| CN104303932A (en) | Guangxi sweet tea cultivating method | |
| CN102498783B (en) | Wild cryptotaenia japonica seed dormancy breaking method | |
| CN113906861A (en) | Low-temperature stratification germination accelerating method for Cotoneaster multiforus seeds | |
| CN108739370A (en) | A method of it is carried out using ripe lotus embryo fast numerous | |
| CN102640637A (en) | Method for breeding seed konjac from konjac rhizomes | |
| Gray | How Plants Grow | |
| CN107926500B (en) | A kind of high-efficient method for cultivating of tsaoko seedling | |
| CN106576772A (en) | Planting method of selenium-rich peppers | |
| CN105191720B (en) | A kind of cuttage breeding method of francolin tea seedling | |
| CN107466766A (en) | The mating system of Kuerle delicious pear crossbreeding nursery stock | |
| CN104838860B (en) | A kind of Fructus Fragariae Ananssae standardized cultivation method | |
| CN107242134A (en) | The method of soilless culture white pea shoot vegetable | |
| CN109496655B (en) | Seed germination and seedling raising method for camellia azalea | |
| CN101697686A (en) | Method for breeding and cultivating Davidia involucrata | |
| CN102640641A (en) | Method for breeding konjac seeds in winter by using konjac rhizomes | |
| CN106688561A (en) | High-temperature and high-moisture plain region disease-resisting and high-yield konjak planting method | |
| Mathew et al. | Standardization of conventional propagation techniques for four medicinal species of genus Ficus Linn. |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220111 |
|
| RJ01 | Rejection of invention patent application after publication |