CN113896602B - Microbial organic fertilizer - Google Patents

Microbial organic fertilizer Download PDF

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Publication number
CN113896602B
CN113896602B CN202111232563.XA CN202111232563A CN113896602B CN 113896602 B CN113896602 B CN 113896602B CN 202111232563 A CN202111232563 A CN 202111232563A CN 113896602 B CN113896602 B CN 113896602B
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organic fertilizer
phosphorus
microbial organic
fertilizer
soil
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CN113896602A (en
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杨艳
逄焕成
马军伟
俞巧钢
王峰
林辉
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Zhejiang Fengyu Ecological Technology Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
    • C05F5/002—Solid waste from mechanical processing of material, e.g. seed coats, olive pits, almond shells, fruit residue, rice hulls
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
    • C05G3/80—Soil conditioners
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
    • Y02W30/00—Technologies for solid waste management
    • Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Botany (AREA)
  • Environmental & Geological Engineering (AREA)
  • Soil Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Biochemistry (AREA)
  • Fertilizers (AREA)

Abstract

The application provides a microbial organic fertilizer which comprises pantoea ananatis and bacillus cuprapae with the effective viable count ratio of 2:1, cassava residue and humic acid powder, can effectively dissolve phosphorus and inhibit diseases, and can improve crop properties and increase yield and income when used for producing crops such as tomatoes.

Description

Microbial organic fertilizer
Technical Field
The application belongs to the microorganism field and fertilizer field, specifically, this application provides a microbial organic fertilizer who contains Pantoea ananatis and sunken bacillus.
Background
According to statistics, the cultivated land with more than 8 crops in China is in phosphorus deficiency with different degrees, phosphate fertilizers are required to be used for realizing the high yield required by modern agriculture, and common crops such as tomatoes, soybeans, sweet potatoes and the like are all high in phosphorus demand. China is the first major country of world phosphate fertilizer consumption, and the annual phosphate fertilizer consumption is over 1000 million tons. What is not suitable for the situation of land is that the phosphorite resource in China is lack, the reserve of the phosphorite resource is kept at 151.98 hundred million tons, the high-quality phosphorite resource is used up in tens of years, and the phosphate fertilizer increasingly becomes the bottleneck limiting the sustainable development of agriculture in China.
On the other hand, most of the phosphate fertilizer is fixed by the soil after being applied into the soil: in calcareous soil the available phosphorus is converted into apatite with extremely low solubility; iron phosphate and aluminum phosphate are formed in acid soil. While the phosphate fertilizer/phosphate rock is deficient in China, the phosphorus fertilizer used for many years causes the accumulation of phosphorus in soil, the accumulated phosphorus exists in a form of insoluble inorganic phosphorus, is slowly released, and is difficult to utilize by crops, namely, the so-called closed phosphorus or high-stability phosphorus.
The solution of the activation and release of the soil phosphorus is a necessary way to improve the utilization efficiency of the soil phosphorus and the phosphate fertilizer and further realize the sustainable development of agriculture in China. The phosphorus-dissolving microorganism is a beneficial microorganism with the capability of activating the insoluble inorganic phosphorus in the soil, and the application of the phosphorus-dissolving microorganism to activate the insoluble phosphorus in the soil is a world-recognized safe, economic and effective biological measure. At present, most of phosphorus-dissolving bactericides are single-strain bactericides, and the phosphorus-dissolving effect and the applicable soil type are limited.
Disclosure of Invention
Having discovered that a large amount of pantoea ananatis has phosphorus dissolving capacity of different degrees, the inventor tries various pantoea ananatis strains obtained from different ways when researching microbial organic fertilizers, and the pantoea ananatis has the problems of insufficient dissolving performance of insoluble phosphorus, poor application effect in actual soil and the like. Aiming at the problem, the inventor tries the phosphorus dissolving effect of different strain combinations, and finds that the combination of the Pantoea ananatis + the Bacillus pumilus and the Pantoea ananatis + the Trichoderma harzianum in a certain proportion has good phosphorus dissolving effect.
In one aspect, the present application provides a microbial organic fertilizer comprising pantoea ananatis.
Further, the microbial organic fertilizer also comprises cassava dregs and humic acid powder.
Furthermore, the organic matter content of the microbial organic fertilizer is more than or equal to 40 percent, the pH value is 6.5-7.5, and the water content is less than or equal to 30 percent.
Further, the microbial organic fertilizer also comprises bacillus cavendiculatus.
Further, the ratio of the effective viable count of pantoea ananatis to bacillus cuprapae in the microbial organic fertilizer is 2: 1.
Further, the microbial organic fertilizer also comprises trichoderma harzianum.
Further, the ratio of the effective viable count of pantoea ananatis to trichoderma harzianum in the microbial organic fertilizer is 1: 2.
In another aspect, the present application provides a phosphate solubilizing bacteria composition comprising pantoea ananatis and bacillus cratesink having an effective viable count ratio of 2: 1.
On the other hand, the application provides the application of the microbial organic fertilizer or the phosphorus-solubilizing bacterium composition in tomato planting.
In another aspect, the application provides an application of the microbial organic fertilizer or the phosphorus-solubilizing bacteria composition in improvement of phosphorus-deficient soil.
Detailed Description
Strains and culture medium:
pantoea ananatis (Pantoea ananatis): the applicant purchased from Chinese academy of agricultural sciences and then self-preserved and passaged, and the initial source is ATCC 19321;
bacillus cuprinus (bacillus depression): the applicant purchases the product from Chinese academy of agricultural sciences and then self-preserves the product and passes the product, and the initial source of the product is 1.15124 of CGMCC;
aspergillus niger (Aspergillus niger) and Trichoderma harzianum (Trichoderma harzianum): the applicant and the cooperative entity screened and deposited themselves and passaged, and since they were used only as controls in the present application and possibly for subsequent applications, no specific information is disclosed herein.
The Pantoea ananatis and the Bacillus cavendiculatus use respective special culture mediums during passage, and the two bacteria use the same improved beef extract peptone culture medium (potassium dihydrogen phosphate 0.1g, dipotassium hydrogen phosphate 0.1g, ammonium sulfate 2.5g, calcium carbonate 10g, ferrous sulfate 0.005g are added per liter) in the experiment.
And (3) other raw materials:
cassava residues: shandongli Miao Biotech Co Ltd
Humic acid powder: henan Black ecology science and technology Ltd
Main experimental method
Phosphorus dissolution experiment:
using a hardly soluble phosphorus solid culture medium: glucose 10g, MgSO4·7H2O0.4 g, phosphorus source 5g, (NH4)2SO4 0.5g、NaCl0.2g、KCl 0.3g、MnSO4 0.03g、FeSO4·7H20.01g of O, 20g of agar, 0.5g of yeast extract and 1000mL of water. The phosphorus source is calcium phosphate, hydroxyapatite and iron phosphate.
Preparing bacterial suspension with similar OD from the bacterial strain to be detected, and uniformly coating 200 mu L of bacterial suspension in a 6cm culture dish. And (3) culturing at 25 ℃ for 1 day, then beating the strain into a bacterial cake by using a 6mm puncher, inoculating the bacterial cake onto the culture medium plate, and measuring the ratio (D/D) of the diameter (D) of a phosphorus-dissolving ring to the diameter (D) of a bacterial colony after culturing at 25 ℃ for 3 days to determine the phosphorus-dissolving capacity of the strain.
Field experiment:
site of experiment 1: inner Mongolia Hangzhou flag two-way bridge greenhouse
Test soil: the test field is a greenhouse with 8-meter span, the soil is silt irrigation soil, the terrain is flat, the irrigation and drainage is better, the fertility is moderate, and the soil is light saline soil (Table 1).
TABLE 1 nutrient status of the soil tested
Figure BDA0003316601220000031
Fertilizer to be tested: the microbial agent is prepared into granules by Zhejiang Feng Yu ecological science and technology GmbH. The main indexes are as follows: the microorganism indexes are that the strains are Pantoea ananatis and Bacillus dentus, the effective viable count is more than or equal to 0.2 hundred million/g, and the ratio of the effective viable counts of the Pantoea ananatis and the Bacillus dentus is 2: 1; organic matter is more than or equal to 40 percent, pH is 6.5-7.5, and water content is less than or equal to 30 percent.
And (3) test varieties and cultivation modes: the tomato variety is Provence. Adopts a ridging, film-covering and vine-hanging type three-dimensional cultivation mode. 6-7 true leaves of the seedlings are planted in the period of 2 months and 7 days. The large row spacing is 60-70 cm, the small row spacing is 40 cm, the plant spacing is 50-55 cm, and the mu reserved seedling is 2500 strains.
The test design and fertilization method comprises the following steps:
and (3) experimental design: conventional fertilization and basal application of a test microbial organic fertilizer (T1), conventional fertilization and basal application of a microbial organic fertilizer (CK1) of an inactivated fertilizer 1 and conventional fertilization (CK 2). Repeating for 4 times, the cell area is 30m2And random block arrangement, setting up a protection row for 1 meter, and keeping other management consistent.
T1: on the basis of conventional fertilization, basal application is carried out during land preparation, and 30kg of microbial organic fertilizer is applied per mu;
CK 1: on the basis of conventional fertilization, 30kg of equivalent inactivated microbial organic fertilizer is applied in the land preparation;
CK 2: namely conventional fertilization. Soil preparation is carried out 15 days before field planting in 22 days 1 month in 2019, sufficient base fertilizer is applied, 5000kg of fully decomposed farmyard manure, 80kg of heavy calcium superphosphate and 35kg of potassium sulfate are applied per mu in combination of land turning, deep ploughing is carried out for 30 cm, water is deeply poured, soil moisture is preserved, and alkali is pressed. When the first set of fruits grows to be large, critical water and topdressing are poured, and 20kg of compound fertilizer is topdressed per mu each time; watering every 10 days, and topdressing with water after each set of stable fruit seat.
Site of experiment 2: greenhouse of agriculture bureau of prefecture county sunflower Boyuan of inner Mongolia
Test soil: the test field is a greenhouse with 8-meter span, the soil is silt irrigation soil, the terrain is flat, the irrigation and drainage is better, the fertility is moderate, and the soil is light saline soil (Table 2).
TABLE 1 nutrient status of the soil tested
Figure BDA0003316601220000032
Fertilizer to be tested: the microbial agent is prepared into granules by Zhejiang Feng Yu ecological science and technology GmbH. The main indexes are as follows: the microorganism indexes are that the strains are Pantoea ananatis and Bacillus dentus, the effective viable count is more than or equal to 0.2 hundred million/g, and the ratio of the effective viable counts of the Pantoea ananatis and the Bacillus dentus is 2: 1; organic matter is more than or equal to 40 percent, pH is 6.5-7.5, and water content is less than or equal to 30 percent.
Test varieties and cultivation modes: the tomato variety is Provence. Adopts a ridging, film-covering and vine-hanging type three-dimensional cultivation mode. 6-7 true leaves of the seedlings are planted in 2 months and 5 days. The large row spacing is 60-70 cm, the small row spacing is 40 cm, the plant spacing is 50-55 cm, and the mu reserved seedling is 2500 strains.
Test design and fertilization method
Test equipment: conventional fertilization and basal application of a test microbial organic fertilizer (T1), conventional fertilization and basal application of a microbial organic fertilizer (CK1) of an inactivated fertilizer 1 and conventional fertilization (CK 2). Repeating for 4 times, the cell area is 30m2And random block arrangement, setting up a protection row for 1 meter, and keeping other management consistent.
T1: on the basis of conventional fertilization, 30kg of microbial organic fertilizer is applied to each mu of base soil during soil preparation;
CK 1: on the basis of conventional fertilization, 30kg of equivalent inactivated microbial organic fertilizer is applied in the land preparation;
CK 2: namely conventional fertilization. Soil preparation is carried out 15 days before field planting for 20 days in 1 month and 20 days in 2019, sufficient base fertilizer is applied, 5000kg of fully decomposed farmyard manure, 80kg of heavy calcium superphosphate and 35kg of potassium sulfate are applied per mu in combination of field turning, deep ploughing is carried out for 30 cm, water is deeply poured, soil moisture is preserved, and alkali is pressed. When the first set of fruits grows to be large, critical water and topdressing are poured, and 20kg of compound fertilizer is topdressed per mu each time; watering every 10 days, and topdressing with water after each set of stable fruit seat.
Example 1 selection of species in microbial organic fertilizer
According to the research of the prior art, a large number of pantoea ananatis have been found to have different degrees of phosphorus dissolving capacity, and the inventor tries various pantoea ananatis strains obtained from different ways when researching microbial organic fertilizers, which generally has the problems of insufficient dissolving performance of insoluble phosphorus, poor application effect in actual soil and the like. In response to this problem, the inventors tried the phosphate solubilizing effect of different combinations of strains, and the results are shown in the following table (actually verified strains are more, and are not shown fully for many combinations with antagonistic effect and for reasons of confidentiality and continued application):
TABLE 1 phosphorus solubilization Effect of Individual strains and combinations of strains
Figure BDA0003316601220000041
Figure BDA0003316601220000051
". bacterial combinations 200. mu.l of bacterial suspension on the plate consisted of 100. mu.l each of the two bacterial suspensions.
"-" has no soluble phosphorus ring or no measurable and obvious soluble phosphorus ring.
The results show that the Pantoea ananatis and Aspergillus niger to be detected both have good insoluble phosphorus dissolving capacity, and Trichoderma harzianum and Bacillus pumilus show certain phosphorus dissolving performance in screening, but have obvious deficiency in the insoluble phosphorus dissolving capacity. Compared with a single strain, the combination of the pantoea ananatis and the bacillus cuprapae and the trichoderma harzianum obviously improves the phosphorus dissolving capacity of insoluble phosphorus, particularly the phosphorus dissolving capacity of iron phosphate.
Subsequently, the inventors further verified the phosphate solubilizing effect of the combination of pantoea ananatis + bacillus caveolus and pantoea ananatis + trichoderma harzianum in different proportions. The results are shown in table 2:
Figure BDA0003316601220000052
Figure BDA0003316601220000061
"-" has no soluble phosphorus ring or no measurable and obvious soluble phosphorus ring.
The result shows that the pantoea ananatis and bacillus caveolus with the viable count of 2:1 have the best phosphorus dissolving effect.
Example 2 actual fertiliser and formulation and field test results
The granular microbial fertilizer is prepared by mixing pantoea ananatis, bacillus crater (the ratio of the number of viable bacteria is 2:1, the number of the viable bacteria in actual fertilizer detection is more than or equal to 0.2 hundred million/g), cassava residue and humic acid powder (the basic production process comprises the steps of sending qualified raw materials such as the cassava residue and the humic acid powder after fermentation and crushing into a raw material stirrer according to a formula, uniformly mixing, adding quantitative fermented bacteria liquid while stirring, continuously stirring and mixing for 30 minutes, conveying the mixture into a granulator to form granules, conveying the granules into a sieving machine to sieve, returning the granules into the granulator again for granulation after large granules are crushed, packaging the granules into finished products after sampling and detecting each index of the qualified products, and warehousing the finished products with the organic matter content of more than 40%, the pH of 6.5-7.5 and the water content of less than or equal to 30%. The microbial fertilizer and the previously prepared microbial fertilizer of the pineapple panpauli with similar properties (the number of effective viable bacteria in actual fertilizer detection is more than or equal to 0.2 hundred million/g) are used for actual field experiments, and the results are as follows:
hangjinhou flag:
influence of application of microbial organic fertilizer on tomato growth condition
Figure BDA0003316601220000062
Effect of microbial organic fertilizer application on tomato yield
Figure BDA0003316601220000063
Analysis of variance table
Figure BDA0003316601220000064
Figure BDA0003316601220000071
According to the experimental investigation result, compared with the conventional fertilization, the application of the microbial organic fertilizer on the tomatoes has the advantages that the tomatoes have thick and strong stems, the number of the plants and the weight of a single fruit are increased, the fruit cracking is reduced, the incidence rate of the navel rot is reduced, and the vegetative growth and reproductive growth conditions of the tomatoes can be improved.
The comparison of the test yield shows that the application of the microbial organic fertilizer to the tomatoes can greatly improve the yield of the tomatoes, the yield is increased by 11.4 percent, the income is increased by 4197 yuan/mu, and the yield and the efficiency are increased by applying the microbial organic fertilizer produced by Zhejiang Feng yoga ecological science and technology limited company.
Wuyuan county
Influence of application of microbial organic fertilizer on growth condition of tomatoes
Figure BDA0003316601220000072
Effect of microbial organic fertilizer application on tomato yield
Figure BDA0003316601220000073
TABLE 6 ANOVA TABLE
Figure BDA0003316601220000074
Figure BDA0003316601220000081
According to the experimental investigation result, compared with the conventional fertilization, the application of the microbial organic fertilizer on the tomatoes has the advantages that the tomatoes have thick and strong stems, the number of the plants and the weight of a single fruit are increased, the fruit cracking is reduced, the incidence rate of the navel rot is reduced, and the vegetative growth and reproductive growth conditions of the tomatoes can be improved.
The comparison of the test yield shows that the yield of the tomatoes can be remarkably improved by applying the microbial organic fertilizer, the yield is increased by 9.9 percent, the income is increased by 3198 yuan/mu, and the yield and the efficiency are increased by applying the microbial organic fertilizer produced by Zhejiang Feng yoga ecological science and technology limited company.

Claims (5)

1. The application of the phosphorus-solubilizing bacteria composition in preparation of the microbial organic fertilizer is characterized in that the phosphorus-solubilizing bacteria composition consists of pantoea ananatis and bacillus crater with the effective viable count ratio of 2:1, and the effective viable count of the microbial organic fertilizer is more than or equal to 0.2 hundred million/g; the Pantoea ananatis strain is ATCC 19321, and the Depressin bacillus strain is CGMCC 1.15124.
2. The use of claim 1, wherein the microbial organic fertilizer further comprises cassava residue and humic acid powder.
3. The application of claim 2, wherein the organic matter content of the microbial organic fertilizer is more than or equal to 40%, the pH value is 6.5-7.5, and the water content is less than or equal to 30%.
4. Use according to any one of claims 1 to 3, wherein the microbial organic fertilizer is used for tomato cultivation.
5. The use according to any one of claims 1-3, wherein the microbial organic fertilizer is used for improving phosphorus-deficient soil.
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