CN113892642A - Probiotic composition for relaxing bowel - Google Patents
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- CN113892642A CN113892642A CN202110978620.2A CN202110978620A CN113892642A CN 113892642 A CN113892642 A CN 113892642A CN 202110978620 A CN202110978620 A CN 202110978620A CN 113892642 A CN113892642 A CN 113892642A
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- 239000006041 probiotic Substances 0.000 title claims abstract description 44
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 44
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- 230000000529 probiotic effect Effects 0.000 title claims abstract description 35
- 239000000284 extract Substances 0.000 claims abstract description 55
- 241000901050 Bifidobacterium animalis subsp. lactis Species 0.000 claims abstract description 47
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- 241000218236 Cannabis Species 0.000 claims abstract description 33
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Botany (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
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Abstract
The invention discloses a probiotic composition for relaxing bowel, which comprises bifidobacterium lactis and plant extract materials in a mass ratio of 3-60:265-345, wherein the plant extract materials are a mixture of an angelica extract and a fructus cannabis extract. By the specific proportion of the bifidobacterium lactis to the selected angelica extract and the fructus cannabis extract, the angelica extract and the fructus cannabis extract can promote the proliferation of the bifidobacterium lactis and can realize the effect of promoting defecation and relaxing the bowels with a smaller dosage.
Description
Technical Field
The invention relates to the field of food and health-care food, in particular to a probiotic composition for relaxing bowel.
Background
Constipation refers to the reduction of stool frequency, generally less than 3 times per week, with difficulty in defecation and dry stool. Common laxatives include probiotics, fibers, prebiotics, traditional Chinese medicinal materials, a lubricating laxative and the like, but the common laxatives generally have the following problems in the using process:
the product has the advantages that the product has certain effect of relaxing the bowels and small side effect, but most of the product has slow onset time and is difficult to meet the requirement of people suffering from constipation for quickly relieving the constipation.
② the purgative Chinese medicinal herb products can have the function of relaxing bowels in a short time, but the purgative medicaments have more intense action and larger dosage, and need to be used with caution for some weak and weak people. After being eaten by many constipation people, the constipation people are easy to have tolerance, cannot achieve the effect of fundamentally improving the intestinal environment, and are easy to relapse after the eating is stopped; if the food is eaten for a long time, toxic and side effects are easy to generate, and adverse reactions such as diarrhea, black bowel disease and the like are caused.
Third, lubricating purgative agents, such as some oil and fat products, generally require a large dosage to relieve constipation, and cannot relieve constipation and improve the intestinal environment.
CN103652855A discloses a special diet for relaxing bowel, which is prepared by mixing a plurality of probiotics and a plurality of traditional Chinese medicine components, is safe and effective, has small toxic and side effects, takes common food as a main raw material, has low price and is easily accepted by people. The patent document prepares special diet containing probiotics into liquid, capsule, tablet or biscuit and other forms by adding sweetener, black sesame powder, yam powder. On one hand, probiotics are very sensitive to the external environment, and can be quickly activated and attenuated in a liquid environment, so that the efficacy of the probiotics is influenced; on the other hand, the special diet is prepared into solid forms such as capsules and tablets, the traditional Chinese medicine components contained in the special diet are very complex, and under the condition of long-term storage, the survival rate of probiotics is reduced by the complex traditional Chinese medicine components, so that the effect of the probiotics on regulating the intestinal health is influenced.
Disclosure of Invention
In practice, the invention discovers that although angelica, fructus cannabis and bifidobacterium lactis (probiotics) all have a certain effect on improving constipation, when the composition consisting of the angelica, the fructus cannabis and the bifidobacterium lactis is used for treating constipation, components such as organic acid in the angelica and the like can inhibit the proliferation of the bifidobacterium lactis, linoleic acid, linolenic acid and the like in the fructus cannabis can also inhibit the proliferation of the bifidobacterium lactis, so that the curative effect of the composition on the constipation is influenced, and the curative effect is not enhanced, but is possibly poorer than that of the bifidobacterium lactis which is singly used. .
Aiming at the defects in the prior art, the invention provides a probiotic composition for relaxing bowel; the probiotic composition for relaxing bowel comprises bifidobacterium lactis and plant extract materials in a mass ratio of 3-60:265-345, wherein the plant extract materials are a mixture of an angelica extract and a fructus cannabis extract.
The probiotic composition capable of quickly relieving constipation is mainly used for treating qi deficiency type constipation of middle-aged and elderly people. The angelica sinensis extract and the fructus cannabis extract which are adopted by the invention keep effective components capable of promoting the proliferation of probiotics, and reduce components which can influence the proliferation of bifidobacterium lactis, and the angelica sinensis extract and the fructus cannabis extract can promote the proliferation of bifidobacterium lactis at a specific proportion through the scientific proportion of the bifidobacterium lactis, the angelica sinensis extract and the fructus cannabis extract.
Further, the extraction method of the angelica sinensis extract comprises the following steps:
pulverizing radix Angelicae sinensis, extracting, concentrating, sterilizing, and drying to obtain radix Angelicae sinensis extract.
Further, the extraction method of the fructus cannabis extract comprises the following steps:
the fructus cannabis extract is obtained by crushing, extracting, concentrating and drying fructus cannabis.
Further, the mass ratio of the angelica sinensis extract to the fructus cannabis extract is 100-180: 165.
Preferably, the mass ratio of the bifidobacterium lactis to the plant extract material is 3-60: 335.
More preferably, the mass ratio of the bifidobacterium lactis to the plant extract material is 10: 265-345.
Further, the probiotic composition for relaxing bowel is powder, hard capsules or tablets.
Compared with the prior art, the invention has the following beneficial effects:
(1) the prepared probiotic composition for relaxing bowel consists of bifidobacterium lactis and plant extract materials in a specific ratio, wherein the plant extract materials are a mixture of an angelica extract and a fructus cannabis extract, the plant extract materials basically have no influence on the survival rate of the living bacteria of the bifidobacterium lactis, and the living bacteria survival stability of the bifidobacterium lactis is high.
(2) According to the prepared probiotic composition for relaxing bowel, the Chinese angelica extract and the linseed extract can promote the proliferation of bifidobacterium lactis in a specific proportion, so that the effect of promoting defecation and relaxing the bowel can be realized under a smaller using dose, and the synergistic effect of the plant extract material and the bifidobacterium lactis is realized.
Drawings
Fig. 1 is a live bacteria survival rate chart of probiotic composition formulas 1-8 for relaxing bowel according to the invention.
Fig. 2 is a live bacteria survival rate chart of probiotic composition formulas 8-13 for relaxing bowel according to the invention.
Fig. 3 is a graph showing the survival rate of live bacteria in the formula 14-20 of the probiotic composition for relaxing bowel.
Fig. 4 is a comparison graph of defecation times of a formula A and a formula B of the probiotic composition for relaxing bowel.
Fig. 5 is a graph comparing the number of bowel movements during the test period of the probiotic composition formula B test group for relaxing bowel according to the present invention.
Detailed Description
In order to make those skilled in the art better understand the technical solution of the present invention, the technical solution in the embodiment of the present invention will be clearly and completely described below with reference to the drawings in the embodiment of the present invention, and it is obvious that the described embodiment is only a part of the embodiment of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
Preparing an angelica extract: pulverizing Angelica sinensis, extracting with water or ethanol, centrifuging, micro-filtering (ceramic membrane), concentrating (reverse osmosis concentrating membrane), sterilizing, and drying to obtain Angelica sinensis extract.
Preparing fructus cannabis extract: pulverizing fructus Cannabis, extracting with water or ethanol, concentrating, sterilizing, and drying to obtain fructus Cannabis extract.
And (3) testing the survival rate of the viable bacteria: the number of live bacteria of different formulas is detected according to GB 4789.35 food safety national standard food microbiology inspection lactobacillus inspection, and the live bacteria number is divided by a theoretical value to obtain the live bacteria survival rate.
TABLE 1 probiotic composition formula for relaxing bowel
| Substance(s) | |
Formulation 2 | Formulation 3 | |
|
Formulation 6 | Formulation 7 | Formulation 8 |
| Bifidobacterium lactis | 1 | 3 | 3 | 10 | 60 | 60 | 100 | 100 |
| Angelica sinensis extract | 180 | 190 | 180 | 180 | 90 | 100 | 180 | / |
| Fructus Cannabis extract | 165 | 155 | 165 | 165 | 175 | 165 | 165 | / |
The viable bacteria survival rates of different probiotic composition formulas for relaxing bowel are shown in fig. 1, and as can be seen from fig. 1, when the mass ratio of the bifidobacterium lactis to the angelica sinensis extract to the fructus cannabis extract is 3-60: 265-; wherein when the mass ratio of the angelica sinensis extract to the fructus cannabis extract is 100-180:165, the survival rate of viable bacteria of the bifidobacterium lactis in the angelica sinensis extract and the fructus cannabis extract is 92-99%.
TABLE 2 probiotic composition formula for relaxing bowel
| Substance(s) | Formulation 8 | Formulation 9 | Formulation 10 | Formulation 11 | Formulation 12 | Formulation 13 |
| Bifidobacterium lactis | 100 | 1 | 3 | 10 | 60 | 100 |
| Angelica sinensis extract | / | 170 | 170 | 170 | 170 | 170 |
| Fructus Cannabis extract | / | 165 | 165 | 165 | 165 | 165 |
The viable bacteria survival rates of different probiotic composition formulas for relaxing bowel are shown in fig. 2, and it can be seen from fig. 2 that when the mass ratio of the bifidobacterium lactis to the angelica sinensis extract to the fructus cannabis extract is 3-60:335, the viable bacteria survival rate of the bifidobacterium lactis is 93-99%.
TABLE 3 probiotic composition formula for relaxing bowel
| Substance(s) | Formulation 14 | Formulation 15 | Formulation 16 | Formulation 17 | Formulation 18 | Formulation 19 | |
| Bifidobacterium lactis | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
| Angelica sinensis extract | / | 100 | 150 | 180 | 165 | 170 | 200 |
| Fructus Cannabis extract | / | 165 | 100 | 150 | 180 | 190 | 50 |
The viable bacteria survival rates of different probiotic composition formulas for relaxing bowel are shown in fig. 3, and as can be seen from fig. 3, the mass ratio of the bifidobacterium lactis to the angelica sinensis extract to the fructus cannabis extract is 10:265-345, and the viable bacteria survival rate of the composition ratio is 94-99%.
Example 2: test of proliferation effect of bifidobacterium lactis on mass ratio of bifidobacterium lactis to plant extract material
(1) The test method comprises the following steps:
carrying out anaerobic pre-culture on a Bifidobacterium lactis MRS culture medium at 37 ℃ for 24 hours until the OD600 reaches 0.55-0.6, taking 1mL of bacterial liquid for viable count, wherein the corresponding viable addition amount is 3 mg; adding the pre-cultured bacterial liquid into a honeycomb plate special for a full-automatic growth curve analyzer in an inoculation amount of 2%, and simultaneously adding 95% of mMCB (dextrose-free intestinal culture broth), 2% of deoxyenzyme and 1% of plant extract composition in each proportion into each honeycomb plate to serve as a carbon source. After the culture system was completed, an equal volume of sterilized mineral oil was added to the culture system to exclude oxygen. Setting parameters of a full-automatic growth curve analyzer, setting the culture temperature to be 37 ℃, and reading the OD600 value of each culture well for 24 hours after culturing for 24 hours. Meanwhile, collecting the bacterial liquid after 24 hours of culture, extracting DNA, and quantitatively detecting the quantity of the bifidobacterium lactis after 24 hours by using a fluorescent quantitative PCR instrument.
Proliferation multiple is the number of bifidobacterium lactis after plant extraction culture/the number of bifidobacterium lactis after blank control culture.
(2) And (3) testing results:
TABLE 4 test of the proliferative Effect of the probiotic composition formula for relaxing bowel on Bifidobacterium lactis
When the mass ratio of the bifidobacterium lactis to the plant extract material is 3-60:265-345, the plant extract material has good promotion effect on the proliferation of the bifidobacterium lactis.
TABLE 5 test of the proliferative Effect of the probiotic composition formula for relaxing bowel on Bifidobacterium lactis
The proliferation efficacy test method for the formulations of table 5 differs from that of table 4 in that: after culturing the bifidobacterium lactis for 24 hours, the OD600 of the bifidobacterium lactis reaches 0.6-0.7, and 1mL of bacterial liquid is taken for viable count, wherein the corresponding viable count is equivalent to the addition amount of the bifidobacterium lactis of 60 mg.
As can be seen from table 5: when the mass ratio of the bifidobacterium lactis to the plant extract material is 3-60:265-345, the plant extract material has obvious proliferation effect on the bifidobacterium lactis.
Example 3: efficacy testing of probiotic compositions.
(1) The test method comprises the following steps:
the evaluation on the eating effect of the population of the composition for relaxing bowel is carried out according to the health food functional assessment program and test method specification (2003 edition) by taking 3 days as a test period.
The experiment is divided into 3 stages: the method comprises the following steps of recruitment and grouping of tested persons, emptying period and product taking period.
Subject inclusion criteria: (1) the age is 45-75 years old, and male and female are not limited; (2) defecation times per week are less than or equal to 2; (3) decreased defecation frequency and increased stool hardness; (4) clear consciousness, and can take the product and finish the test according to the requirements.
(2) And (3) testing results:
TABLE 6 probiotic composition formula for loosening bowel to relieve constipation
| Ingredients | Formulation A, mg | Formulation B, mg |
| Bifidobacterium lactis | 6 | / |
| Angelica sinensis extract | 160 | 160 |
| Fructus Cannabis extract | 150 | 150 |
The efficacy test results of the probiotic compositions are shown in fig. 4 and fig. 5, and in fig. 4, it can be seen that the probiotic composition formula a (2.82 ± 1.19) has a significant difference (P <0.05) in the number of stools compared to formula B (1.88 ± 0.78). In the intestine-moistening and bowel-relaxing probiotic composition with a specific proportion range, the composition consisting of the bifidobacterium lactis, the angelica sinensis extract and the fructus cannabis extract has a good effect of promoting defecation and relaxing bowels. As can be seen in fig. 5, the number of bowel movements (2.29 ± 0.45, P <0.001) was significantly different in the test period of the formula B test group (P <0.001) compared to the blank period (0.71 ± 0.45) after 3 days of administration.
Finally, it should be noted that the above-mentioned embodiments are only used for illustrating the technical solutions of the present invention and not for limiting the same, and although the present invention is described in detail with reference to the above-mentioned embodiments, it should be understood by those skilled in the art that the modifications and equivalents of the specific embodiments of the present invention can be made by those skilled in the art after reading the present specification, but these modifications and variations do not depart from the scope of the claims of the present application.
Claims (7)
1. The probiotic composition for relaxing bowel is characterized by comprising bifidobacterium lactis and a plant extract material in a mass ratio of 3-60:265-345, wherein the plant extract material is a mixture of an angelica extract and a fructus cannabis extract.
2. The bowel relaxing probiotic composition according to claim 1, wherein the angelica sinensis extract is extracted by the following method:
pulverizing Angelica sinensis, extracting with water or ethanol, concentrating, sterilizing, and drying to obtain Angelica sinensis extract.
3. The bowel relaxing probiotic composition according to claim 1, wherein the fructus cannabis extract is extracted by the following method:
pulverizing fructus Cannabis, extracting with water or ethanol, concentrating, and drying to obtain fructus Cannabis extract.
4. The bowel relaxing probiotic composition according to claim 1, wherein the mass ratio of the angelica sinensis extract to the fructus cannabis extract is 100-180: 165.
5. The bowel relaxing probiotic composition according to claim 1, wherein the mass ratio of the bifidobacterium lactis to the plant extract material is 3-60: 335.
6. The probiotic composition for relaxing bowels according to claim 1, wherein the mass ratio of the bifidobacterium lactis to the plant extract material is 10: 265-345.
7. The bowel relaxing probiotic composition according to claim 1, which is in the form of a powder, hard capsule or tablet.
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| CN107115381A (en) * | 2017-05-03 | 2017-09-01 | 贵阳中医学院 | Fructus cannabis extract solution, fructus cannabis Yoghourt and preparation method thereof |
| CN110317761A (en) * | 2019-07-23 | 2019-10-11 | 广东益可维健康科技有限公司 | A kind of bifidobacterium lactis and its application |
| CN112076170A (en) * | 2020-08-20 | 2020-12-15 | 浙江康乐药业股份有限公司 | Tablet with function of relaxing bowels |
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