CN113892642B - Probiotic composition for relaxing bowel - Google Patents
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- CN113892642B CN113892642B CN202110978620.2A CN202110978620A CN113892642B CN 113892642 B CN113892642 B CN 113892642B CN 202110978620 A CN202110978620 A CN 202110978620A CN 113892642 B CN113892642 B CN 113892642B
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- 239000000203 mixture Substances 0.000 title claims abstract description 75
- 239000006041 probiotic Substances 0.000 title claims abstract description 42
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 42
- 230000002040 relaxant effect Effects 0.000 title claims abstract description 33
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 32
- 239000000284 extract Substances 0.000 claims abstract description 53
- 241000901050 Bifidobacterium animalis subsp. lactis Species 0.000 claims abstract description 47
- 229940009289 bifidobacterium lactis Drugs 0.000 claims abstract description 47
- 241000218236 Cannabis Species 0.000 claims abstract description 28
- 239000000419 plant extract Substances 0.000 claims abstract description 20
- 239000000463 material Substances 0.000 claims abstract description 17
- 241000125175 Angelica Species 0.000 claims abstract description 15
- 235000001287 Guettarda speciosa Nutrition 0.000 claims abstract description 15
- 241000382455 Angelica sinensis Species 0.000 claims abstract description 13
- 206010010774 Constipation Diseases 0.000 claims description 11
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 8
- 238000001035 drying Methods 0.000 claims description 6
- 238000010298 pulverizing process Methods 0.000 claims description 6
- 238000000605 extraction Methods 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 239000009188 angelicae sinensis extract Substances 0.000 claims description 3
- 239000007902 hard capsule Substances 0.000 claims description 2
- 230000000694 effects Effects 0.000 abstract description 20
- 230000035755 proliferation Effects 0.000 abstract description 13
- 230000001737 promoting effect Effects 0.000 abstract description 6
- 230000029142 excretion Effects 0.000 abstract description 2
- 238000009472 formulation Methods 0.000 description 34
- 238000012360 testing method Methods 0.000 description 16
- 241000894006 Bacteria Species 0.000 description 15
- 230000004083 survival effect Effects 0.000 description 15
- 239000003814 drug Substances 0.000 description 8
- 235000013305 food Nutrition 0.000 description 7
- 239000007788 liquid Substances 0.000 description 6
- 244000025254 Cannabis sativa Species 0.000 description 5
- 235000012766 Cannabis sativa ssp. sativa var. sativa Nutrition 0.000 description 5
- 235000012765 Cannabis sativa ssp. sativa var. spontanea Nutrition 0.000 description 5
- 235000009120 camo Nutrition 0.000 description 5
- 235000005607 chanvre indien Nutrition 0.000 description 5
- 230000013872 defecation Effects 0.000 description 5
- 239000011487 hemp Substances 0.000 description 5
- 230000001580 bacterial effect Effects 0.000 description 4
- 235000005911 diet Nutrition 0.000 description 4
- 230000037213 diet Effects 0.000 description 4
- 229940079593 drug Drugs 0.000 description 4
- 230000000968 intestinal effect Effects 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 239000002775 capsule Substances 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 2
- 239000008141 laxative Substances 0.000 description 2
- 229940125722 laxative agent Drugs 0.000 description 2
- 230000001050 lubricating effect Effects 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 235000013406 prebiotics Nutrition 0.000 description 2
- 230000002829 reductive effect Effects 0.000 description 2
- 239000003826 tablet Substances 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- OYHQOLUKZRVURQ-NTGFUMLPSA-N (9Z,12Z)-9,10,12,13-tetratritiooctadeca-9,12-dienoic acid Chemical compound C(CCCCCCC\C(=C(/C\C(=C(/CCCCC)\[3H])\[3H])\[3H])\[3H])(=O)O OYHQOLUKZRVURQ-NTGFUMLPSA-N 0.000 description 1
- 206010067484 Adverse reaction Diseases 0.000 description 1
- 241000186000 Bifidobacterium Species 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
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- 240000006240 Linum usitatissimum Species 0.000 description 1
- 235000004431 Linum usitatissimum Nutrition 0.000 description 1
- 230000006838 adverse reaction Effects 0.000 description 1
- DTOSIQBPPRVQHS-PDBXOOCHSA-N alpha-linolenic acid Chemical compound CC\C=C/C\C=C/C\C=C/CCCCCCCC(O)=O DTOSIQBPPRVQHS-PDBXOOCHSA-N 0.000 description 1
- 235000020661 alpha-linolenic acid Nutrition 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000002238 attenuated effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 235000015895 biscuits Nutrition 0.000 description 1
- 235000007215 black sesame Nutrition 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000000919 ceramic Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000004426 flaxseed Nutrition 0.000 description 1
- 235000003599 food sweetener Nutrition 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 230000007413 intestinal health Effects 0.000 description 1
- 235000014655 lactic acid Nutrition 0.000 description 1
- 239000004310 lactic acid Substances 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 229960004488 linolenic acid Drugs 0.000 description 1
- KQQKGWQCNNTQJW-UHFFFAOYSA-N linolenic acid Natural products CC=CCCC=CCC=CCCCCCCCC(O)=O KQQKGWQCNNTQJW-UHFFFAOYSA-N 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 239000002480 mineral oil Substances 0.000 description 1
- 235000010446 mineral oil Nutrition 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 150000007524 organic acids Chemical class 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
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- 238000003753 real-time PCR Methods 0.000 description 1
- 238000001223 reverse osmosis Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000003765 sweetening agent Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/135—Bacteria or derivatives thereof, e.g. probiotics
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Botany (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses a probiotic composition for relaxing bowel, which comprises bifidobacterium lactis and plant extract materials in a mass ratio of 3-60:265-345, wherein the plant extract materials are a mixture of angelica sinensis extract and fructus cannabis extract. Through the specific proportion of the bifidobacterium lactis, the selected angelica extract and the selected fructus cannabis extract, the angelica extract and the fructus cannabis extract can promote the proliferation of the bifidobacterium lactis, and the effect of promoting the excretion and relaxing the bowels can be realized under a relatively small dosage.
Description
Technical Field
The invention relates to the field of foods and health-care foods, in particular to a probiotic composition for relaxing bowel.
Background
Constipation refers to a decrease in the number of stools, generally less than 3 times per week, with difficulty in defecation and dry stool. Common cathartic drugs such as probiotics, fibers, prebiotics, traditional Chinese medicinal materials, lubricating laxatives and the like, but the common cathartic drugs generally have the following problems in the use process:
(1) the unilateral or compound bowel relaxing products such as probiotics, fibers, prebiotics and the like have certain bowel relaxing effects and small side effects, but most of the products have slower onset time, and are difficult to meet the rapid relief demands of constipation people.
(2) Although the traditional Chinese medicine products with purgation function can play a role in relaxing bowels in a short time, the purgation medicines have a relatively strong effect, and the dosage is relatively large, so that the purgation medicines are required to be used with cautions for some people with weak body and weak body. Many people with constipation easily develop tolerance after eating, can not achieve the effect of fundamentally improving the intestinal environment, and is easy to relapse after stopping eating; if the food is eaten for a long time, the food is easy to produce some toxic and side effects, and causes some adverse reactions, such as diarrhea, jejunosis and the like.
(3) Lubricating laxatives, such as some greasy products, generally require a relatively large dosage to relieve constipation, and cannot follow up to relieve constipation, improving intestinal environment.
CN103652855a discloses a special diet for relaxing bowel, which is prepared by mixing a plurality of probiotics and a plurality of traditional Chinese medicine components, is safe and effective, has small toxic and side effects, takes common food as main raw materials, has low price, and is easy to accept by people. The patent document prepares special diet containing probiotics into liquid, capsules, tablets or forms of biscuits by adding sweetener and black sesame powder, yam powder and the like. On the one hand, probiotics are very sensitive to the external environment, and can be quickly activated and attenuated in the liquid environment, so that the efficacy of the probiotics is affected; on the other hand, the special diet is prepared into solid forms such as capsules, tablets and the like, the traditional Chinese medicine components contained in the special diet are very complex, and under the condition of long-term storage, the survival rate of probiotics can be reduced by the complex traditional Chinese medicine components, so that the effect of the probiotics on intestinal health regulation is affected.
Disclosure of Invention
In practice, it has been found that although the Chinese angelica, the hemp seed and the bifidobacterium lactis (probiotics) all have a certain effect on improving constipation, when the composition comprising the Chinese angelica, the hemp seed and the bifidobacterium lactis is used for treating constipation, organic acid and other components in the Chinese angelica can inhibit proliferation of the bifidobacterium lactis, linoleic acid, linolenic acid and the like in the hemp seed can also inhibit proliferation of the bifidobacterium lactis, so that the curative effect of the composition on constipation is affected, and the curative effect is not enhanced, but rather the effect is worse than that of the bifidobacterium lactis alone. .
Aiming at the defects in the prior art, the invention provides a probiotic composition for relaxing bowel; the probiotic composition for relaxing bowel comprises bifidobacterium lactis and plant extract materials in a mass ratio of 3-60:265-345, wherein the plant extract materials are a mixture of angelica sinensis extract and fructus cannabis extract.
The probiotic composition capable of rapidly relieving constipation is mainly aimed at qi deficiency type constipation of middle-aged and elderly people. The angelica extract and the fructus cannabis extract adopted by the invention retain the effective components capable of promoting the proliferation of probiotics, reduce the components possibly influencing the proliferation of bifidobacterium lactis, promote the proliferation of bifidobacterium lactis by the scientific proportion of the bifidobacterium lactis and the angelica extract and the fructus cannabis extract under a specific proportion, and realize the effect of promoting the excretion and relaxing the bowels under a relatively small dosage.
Further, the extraction method of the angelica sinensis extract comprises the following steps:
pulverizing radix Angelicae sinensis, extracting, concentrating, sterilizing, and drying to obtain radix Angelicae sinensis extract.
Further, the extraction method of the fructus cannabis extract comprises the following steps:
fructus Cannabis extract is obtained by pulverizing, extracting, concentrating, and drying fructus Cannabis.
Further, the mass ratio of the angelica extract to the fructus cannabis extract is 100-180:165.
Preferably, the mass ratio of the bifidobacterium lactis to the plant extract is 3-60:335.
More preferably, the mass ratio of the bifidobacterium lactis to the plant extract is 10:265-345.
Further, the probiotic composition for relaxing bowel is powder, hard capsules or tablets.
Compared with the prior art, the invention has the following beneficial effects:
(1) The probiotic composition for relaxing bowel prepared by the invention is composed of bifidobacterium lactis and plant extract materials in a specific proportion, wherein the plant extract materials are a mixture of angelica sinensis extract and fructus cannabis extract, the plant extract materials have no influence on the survival rate of viable bacteria of bifidobacterium lactis, and the survival stability of viable bacteria of bifidobacterium lactis is high.
(2) According to the probiotic composition for relaxing bowel, provided by the invention, under a specific proportion, the angelica sinensis extract and the linseed extract can promote proliferation of bifidobacterium lactis, so that the effect of promoting defecation can be realized under a relatively small dosage, and the synergistic effect of plant extract materials and bifidobacterium lactis is realized.
Drawings
FIG. 1 is a graph of viable bacteria survival for the probiotic compositions formulas 1-8 of the present invention for relaxing bowel.
FIG. 2 is a graph of viable bacteria survival for the probiotic composition formulas 8-13 of the present invention for relaxing bowel.
FIG. 3 is a graph of viable bacteria survival for the probiotic composition formulas 14-20 of the present invention for relaxing bowel.
FIG. 4 is a graph showing the comparison of stool frequency for formulation A and formulation B of the probiotic compositions of the present invention for relaxing bowel.
Figure 5 is a graph comparing the number of bowel movements in the blank period with the test period for the test group of probiotic composition formulation B for relaxing bowel in accordance with the present invention.
Detailed Description
In order to make the technical solution of the present invention better understood by those skilled in the art, the technical solution of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, shall fall within the scope of the present invention.
Example 1
Preparing an angelica extract: pulverizing radix Angelicae sinensis, extracting with water or ethanol, centrifuging, microfiltering (ceramic membrane), concentrating (reverse osmosis concentrating membrane), sterilizing, and drying to obtain radix Angelicae sinensis extract.
Preparation of fructus Cannabis extract: pulverizing fructus Cannabis, extracting with water or ethanol, concentrating, sterilizing, and drying to obtain fructus Cannabis extract.
Testing of viable bacteria survival rate: detecting the viable count of different formulas according to GB 4789.35 food safety national Standard food microbiology inspection lactic acid bacteria inspection, and dividing the viable count by a theoretical value to obtain the viable count.
TABLE 1 probiotic composition formulation for relaxing bowel
| Substance (B) | Formulation 1 | Formulation 2 | Formulation 3 | Formulation 4 | Formulation 5 | Formulation 6 | Formulation 7 | Formulation 8 |
| Bifidobacterium lactis | 1 | 3 | 3 | 10 | 60 | 60 | 100 | 100 |
| Angelica sinensis extract | 180 | 190 | 180 | 180 | 90 | 100 | 180 | / |
| Fructus cannabis extract | 165 | 155 | 165 | 165 | 175 | 165 | 165 | / |
The viable bacteria survival rate of the probiotic composition formula with different bowel relaxing effect is shown in figure 1, and as can be seen from figure 1, when the mass ratio of the bifidobacterium lactis to the angelica extract and the fructus cannabis extract is 3-60:265-345, the angelica extract and the fructus cannabis extract have little influence on the viable bacteria survival rate of the bifidobacterium lactis, and the viable bacteria survival rate is 90% -99%; wherein, when the mass ratio of the angelica extract to the hemp seed extract is 100-180:165, the survival rate of viable bacteria of the bifidobacterium lactis in the angelica extract and the hemp seed extract is 92-99%.
TABLE 2 probiotic composition formulation for relaxing bowel
| Substance (B) | Formulation 8 | Formulation 9 | Formulation 10 | Formulation 11 | Formulation 12 | Formulation 13 |
| Bifidobacterium lactis | 100 | 1 | 3 | 10 | 60 | 100 |
| Angelica sinensis extract | / | 170 | 170 | 170 | 170 | 170 |
| Fructus cannabis extract | / | 165 | 165 | 165 | 165 | 165 |
The viable bacteria survival rate of the probiotic composition formula with different bowel relaxing effect is shown in figure 2, and the mass ratio of the bifidobacterium lactis to the angelica extract and the fructus cannabis extract is 3-60:335, as can be seen from figure 2, the viable bacteria survival rate of the bifidobacterium lactis is 93-99%.
TABLE 3 probiotic composition formulation for relaxing bowel
| Substance (B) | Formulation 14 | Formulation 15 | Formulation 16 | Formulation 17 | Formulation 18 | Formulation 19 | Formulation 20 |
| Bifidobacterium lactis | 10 | 10 | 10 | 10 | 10 | 10 | 10 |
| Angelica sinensis extract | / | 100 | 150 | 180 | 165 | 170 | 200 |
| Fructus cannabis extract | / | 165 | 100 | 150 | 180 | 190 | 50 |
The viable bacteria survival rate of the probiotic composition formulas with different bowel relaxing functions is shown in figure 3, and the mass ratio of bifidobacterium lactis to the angelica extract and the fructus cannabis extract is 10:265-345, and the viable bacteria survival rate of the combination ratio is 94% -99% as can be seen from figure 3.
Example 2: test of proliferation effect of bifidobacterium lactis and plant extract material by mass ratio
(1) The testing method comprises the following steps:
anaerobic pre-culturing the bifidobacterium lactis MRS culture medium for 24 hours at 37 ℃ to ensure that the OD600 of the bifidobacterium lactis MRS culture medium reaches 0.55-0.6, taking 1mL of bacterial liquid for viable count, wherein the corresponding viable count is 3mg; the pre-cultured bacterial liquid is added into a special honeycomb plate of a full-automatic growth curve analyzer in an inoculum size of 2%, and simultaneously 95% of mMCB (glucose-free intestinal culture broth), 2% of deoxidizing enzyme and 1% of plant extract composition in each proportion are added into each honeycomb plate as carbon sources. After the culture system is completed, an equal volume of sterilized mineral oil is added to the culture system to sequester oxygen. Parameters of a full-automatic growth curve analyzer were set, the culture temperature was set to 37 ℃, and after 24 hours of culture, the value of OD600 of each culture well was read for 24 hours. Meanwhile, the bacterial liquid after 24 hours of culture is collected and DNA is extracted, and the quantity of bifidobacterium lactis after 24 hours is quantitatively detected by a fluorescent quantitative PCR instrument.
Multiplication fold = number of bifidobacterium lactis after extraction culture/number of bifidobacterium lactis after blank culture.
(2) Test results:
TABLE 4 test of the proliferation effect of probiotic composition formulation for relaxing bowel on bifidobacteria lactis
When the mass ratio of the bifidobacterium lactis to the plant extract material is 3-60:265-345, the plant extract material has a good promoting effect on proliferation of the bifidobacterium lactis.
TABLE 5 test of the proliferation effect of probiotic composition formulation for relaxing bowel on bifidobacterium lactis
The test method of the proliferation effect of the formulation of table 5 differs from that of table 4 in that: after bifidobacterium lactis is cultured for 24 hours, the OD600 of the bifidobacterium lactis is enabled to reach 0.6-0.7, 1mL of bacterial liquid is taken for viable count, and the corresponding viable count is equivalent to 60mg of bifidobacterium lactis.
From table 5 it can be seen that: when the mass ratio of the bifidobacterium lactis to the plant extract material is 3-60:265-345, the plant extract material has obvious proliferation effect on the bifidobacterium lactis.
Example 3: efficacy testing of probiotic compositions.
(1) The testing method comprises the following steps:
the evaluation of the crowd eating effect of the bowel relaxing composition is carried out according to the health food functional evaluation program and test method Specification (2003 edition) by taking 3 days as a test period.
The experiment is divided into 3 stages: (1) the subjects were recruited and grouped, (2) the period of evacuation, (3) the period of product intake.
Subject inclusion criteria: (1) age 45-75 years old, not limited for men and women; (2) the number of times of defecation per week is less than or equal to 2; (3) a reduced number of bowel movements and increased stool hardness; (4) Clear consciousness, and can take the product according to the requirement and finish the test.
(2) Test results:
TABLE 6 probiotic composition formulation for relaxing bowel
| Proportioning materials | Formulation A, mg | Formula B, mg |
| Bifidobacterium lactis | 6 | / |
| Angelica sinensis extract | 160 | 160 |
| Fructus cannabis extract | 150 | 150 |
Efficacy test results of the probiotic compositions are shown in fig. 4 and 5, and it can be seen in fig. 4 that probiotic composition formulation a (2.82±1.19) was significantly different in defecation times (P < 0.05) than formulation B (1.88±0.78). Among the probiotic compositions with specific proportion range, the composition composed of the bifidobacterium lactis, the angelica extract and the fructus cannabis extract has good effect of promoting defecation. As can be seen in fig. 5, after taking for 3 days, there was a significant difference in the number of bowel movements (2.29±0.45, P < 0.001) in the test period of the formula B test group compared to the blank period (0.71±0.45).
Finally, it should be noted that the above-mentioned embodiments are only for illustrating the technical solution of the present invention and not for limiting the same, and although the present invention has been described in detail with reference to the above-mentioned embodiments, it should be understood by those skilled in the art that modifications and equivalents may be made to the specific embodiments of the present invention after reading the present specification, and these modifications and variations do not depart from the scope of the invention as claimed in the pending claims.
Claims (4)
1. The probiotic composition for relaxing bowel is characterized by comprising bifidobacterium lactis and plant extract materials in a mass ratio of 3-60:265-345, wherein the plant extract materials are a mixture of angelica extract and fructus cannabis extract, and the mass ratio of the angelica extract to the fructus cannabis extract is 100-180:165;
the extraction method of the angelica sinensis extract comprises the following steps:
pulverizing radix Angelicae sinensis, extracting with water or ethanol, concentrating, sterilizing, and drying to obtain radix Angelicae sinensis extract;
the extraction method of the fructus cannabis extract comprises the following steps:
pulverizing fructus Cannabis, extracting with water or ethanol, concentrating, and drying to obtain fructus Cannabis extract.
2. The probiotic composition for relaxing bowel according to claim 1, wherein the mass ratio of bifidobacterium lactis to plant extract is 3-60:335.
3. The probiotic composition for loosening bowel to relieve constipation according to claim 1, wherein the mass ratio of bifidobacterium lactis to plant extract is 10:265-345.
4. The probiotic composition for relaxing bowel according to claim 1, which is a powder, a hard capsule or a tablet.
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| CN102895470A (en) * | 2011-07-29 | 2013-01-30 | 闫鹏宇 | Preparation method for aloe pill capable of loosening bowel to relieve constipation |
| CN107115381A (en) * | 2017-05-03 | 2017-09-01 | 贵阳中医学院 | Fructus cannabis extract solution, fructus cannabis Yoghourt and preparation method thereof |
| CN110317761A (en) * | 2019-07-23 | 2019-10-11 | 广东益可维健康科技有限公司 | A kind of bifidobacterium lactis and its application |
| CN112076170A (en) * | 2020-08-20 | 2020-12-15 | 浙江康乐药业股份有限公司 | Tablet with function of relaxing bowels |
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