CN113876657A

CN113876657A - Application of bauhinia extract in oral care product

Info

Publication number: CN113876657A
Application number: CN202111274799.XA
Authority: CN
Inventors: 李江平; 唐红艳; 黄晓燕; 王小敏; 许逊; 劳洁; 许静
Original assignee: LIUZHOU LMZ CO Ltd
Current assignee: LIUZHOU LMZ CO Ltd
Priority date: 2021-10-29
Filing date: 2021-10-29
Publication date: 2022-01-04
Anticipated expiration: 2041-10-29
Also published as: CN113876657B

Abstract

The invention provides an application of a bauhinia extract in an oral care product, wherein the bauhinia extract is added into the oral care product as an active ingredient for clearing heat, cooling blood, inhibiting bacteria, stopping bleeding and preventing gingival bleeding. The cost of the bauhinia flowers is low, the preparation process of the bauhinia flower extract is simple, and a more economical and practical choice is provided for preparing oral care products for clearing heat, removing internal heat and preventing gingival bleeding.

Description

Application of bauhinia extract in oral care product

Technical Field

The invention relates to the technical field of oral care products, in particular to application of a bauhinia extract in oral care products.

Background

Chinese redbud: the traditional Chinese medicine named Laobaila officinalis is flower of Bauhinia variegata L of leguminous plant, pounding, harvesting in summer, and drying in the sun, and has the effects of clearing heat, cooling blood, dispelling wind and removing toxic substances, and the main components of the redbud extract are rutin, lacrimol, kaempferol and other flavonoids.

In recent years, natural extracts, particularly extracts of traditional Chinese medicines in China, are used as active ingredients to be applied to oral care products to solve oral related problems, and become hot spots in the market and pursuits of consumers. The oral care product is an indispensable cleaning, nursing and health care product in daily life of people, and the products of the oral care product comprise toothpaste, mouthwash and the like, and are mainly used for keeping the oral cavity and teeth clean, promoting breath freshness, nursing gingiva, maintaining a good micro-environment of the oral cavity, relieving oral discomfort symptoms and the like.

According to the fourth national oral health epidemiological survey result in China, the periodontal disease is the most common and frequent disease of the oral cavity and the common oral problems of excessive internal heat and gingival bleeding of the oral cavity, and the main reasons are irregular diet and excessive bleedingFatigueAnd the gum is inflamed due to pungent and pungent stimulation when being eaten up all the time, and the gum is inflamed.

Disclosure of Invention

The invention provides an application of a bauhinia extract in oral care products, and the bauhinia extract has the effects of clearing heat, cooling blood, inhibiting bacteria, stopping bleeding and preventing gingival bleeding.

In order to achieve the purpose, the technical scheme of the invention is as follows:

the Chinese redbud extract is used as an active ingredient for clearing heat, cooling blood, inhibiting bacteria, stopping bleeding and preventing gingival bleeding and is added into an oral care product.

Preferably, the mass percentage of the bauhinia extract in the oral care product is 0.01-2.0%.

Preferably, the preparation process of the bauhinia extract comprises the following steps:

adding 70-80 vol.% ethanol which is 2-5 times of the weight of the Chinese redbud, performing reflux extraction at 70-80 ℃, filtering after extraction, concentrating the filtrate under reduced pressure at the temperature of less than or equal to 50 ℃ to obtain an extract, and performing vacuum drying to obtain the Chinese redbud extract.

More preferably, the weight of ethanol is 2 times that of the Chinese redbud flower, and the concentration is 75 vol.%.

More preferably, the content of total flavonoids in the extract of bauhinia is 10-20 wt%.

An oral care product with a gingival bleeding preventing effect contains 0.01-2.0% of a bauhinia extract.

Preferably, the oral care product is a toothpaste.

Preferably, the oral care implement is a mouthwash.

The invention provides an application of a Chinese redbud extract in an oral care product, the Chinese redbud extract is added into the oral care product, and has the effects of cooling blood, inhibiting bacteria, stopping bleeding, clearing heat, removing fire and preventing gingival bleeding, the production process is convenient to control, the content and the quality of active ingredients of each batch of products are more uniform, stable and safe, and the effect is more prominent.

The cost of the Chinese redbud is low, the preparation process of the Chinese redbud extract is simple, a more economical and practical choice is provided for preparing the oral care product for clearing heat, removing internal heat and preventing gingival bleeding, and the Chinese redbud extract is more favorable for being accepted by consumers.

Detailed Description

The present invention is further illustrated by the following specific examples, but the scope of the present invention is not limited to the following examples.

Firstly, extracting flavonoid active ingredient from Chinese redbud

The preparation process of the bauhinia extract comprises the following steps:

1. weighing 10 parts by weight of Chinese redbud, and adding 20 parts by weight of 75vol.% ethanol;

2. reflux extracting at 70-80 deg.C;

3. concentrating the filtrate under reduced pressure at 50 deg.C or lower to obtain extract, and vacuum drying.

The content of the total flavone of the prepared Chinese redbud extract is 10-20%, and the Chinese redbud extract is added into a product as an active ingredient with the effects of clearing heat and cooling blood in the process of preparing an oral care product.

Second, the application of the Chinese redbud extract in oral care products (taking the preparation in toothpaste as an example)

The toothpaste in examples 1 to 4 comprises the following raw materials in percentage by weight as shown in table 1:

TABLE 1 toothpaste containing the extract of bauhinia flowers

Third, bacteriostatic effect of toothpaste containing Chinese redbud extract

Staphylococcus aureus widely lives in nature, is carried in air, water and dust, is a pyogenic microorganism with wide transmission and strong pathogenicity, can cause oral pyogenic infection, and is a main pathogenic bacterium of oral diseases. Coli can cause various urinary infections, acute diarrhea and the like outside the intestinal tract. Porphyromonas gingivalis is a gram-negative anaerobic coccobacillus which is not glycolytic and is one of pathogenic bacteria of periodontal diseases. Modern medical experiments prove that streptococcus mutans in dental plaque can generate acid in metabolism, corrode hard tissues of teeth, generate caries and are main pathogenic microorganisms causing caries.

The test method comprises the following steps:

the test is carried out according to the method for testing the bacteriostatic performance of the dissolution type antibacterial (bacteriostatic) product of the disinfection technical specification 2002 edition-2.1.11.3.2.

The 24-hour slant culture of the test bacteria was washed with PBS to prepare a bacterial suspension (the required concentration was 100. mu.l of the test bacteria was dropped on a control sample or 5ml of the sample, and the number of recovered bacteria was 1X 104 cfu/plate or ml-9X 104 cfu/plate or ml).

10g of the toothpaste sample of example 2 was added to 100mL of physiological saline, and mixed well to prepare a stock solution as a sample solution, and the sample solution (5mL) was taken in 3 tubes.

Taking the bacterial suspension, respectively dripping 100 mu l of the bacterial suspension into each sample liquid, uniformly coating/mixing, starting timing, acting for 3min, respectively putting a sample slice or sample liquid (0.5ml) into a test tube containing 5ml of PBS by using sterile forceps, fully and uniformly mixing, properly diluting, then taking 2-3 dilutions, respectively sucking 0.5ml, placing in 2 plates, pouring by using 15ml of nutrient agar medium (bacteria) or Sabouraud's agar medium (yeast) cooled to 40-45 ℃, rotating the plates to fully and uniformly, turning over the plates after agar is solidified, culturing for 48h (bacteria) or 72h (yeast) at 35 +/-2 ℃, and counting viable bacterial colonies.

Each test bacterium experiment was repeated 3 times, and the inhibition rate was calculated as follows:

in the formula: x is the bacteriostasis rate,%; n is a radical of_CMean colony number, cfu/plate, for control samples; n is a radical of_SThe average colony count of the samples to be tested, cfu/plate.

Table 2 antibacterial effect test results of toothpaste containing Chinese redbud extract

The antibacterial tests show that the toothpaste containing the Chinese redbud extract has antibacterial effect on escherichia coli, staphylococcus aureus, candida albicans and streptococcus mutans.

Fourth, the toothpaste containing the Chinese redbud extract has the hemostatic effect on the rabbit liver local wound injury bleeding

120 healthy New Zealand rabbits (30 rabbits each group) are taken, the weight is 2.0-2.5 kg, and the rabbits can be used for both males and females. Slowly injecting into ear vein of 30mg/kg of 3% sodium pentobarbital, anesthetizing, supining, fixing on operating table, making the middle incision of abdomen about 6-8 cm long, exposing and fixing liver, cutting liver left lobe with surgical scissors to obtain liver tissue with length of about 1cm, width of about 1cm, and height of about 1cm,

the wound surface is bleeding like spraying. After the sprayed blood is wiped off, 1g of a sample to be tested is immediately coated on the part of the wound surface, gauze is covered, a weight of 200g is used for compressing for 1min, the weight is removed after 1min, the hemostasis effect is observed, bleeding does not occur, if bleeding still occurs, the sample to be tested is coated and then is continuously pressurized, the observation is carried out once every 30s, the hemostasis time is recorded, if bleeding still occurs after exceeding 3min, the hemostasis time is recorded as the hemostasis failure, and the hemostasis time is recorded as more than 3 min. After each sample test, the residual test substance is washed away by physiological saline at 37 ℃ so that the wound surface bleeds again, and then the next test substance sample is tested. Four tests of hemostasis test of liver local wound injury are carried out on each rabbit, and each test sample is applied according to the Latin prescription procedure, so that the influence of incision difference and medication sequence of liver local wound injury is reduced. The hemostatic experiment of the rabbit liver local wound injury bleeding is carried out for 20 times in total for each test object sample. The hemostatic effect is marked as F by the reciprocal score method (10/T), hemostasis is not achieved for more than 3min, and no F score is marked as 0.

TABLE 3 hemostatic effect (X + -S) of toothpaste containing Cercis chinensis extract on rabbit liver local wound bleeding

Note: comparison with starch group: # # P < 0.001, P > 0.05.

Compared to a toothpaste blank matrix containing bauhinia flowers toothpaste: p < 0.001, Δ P > 0.05.

The experimental result shows that the toothpaste containing the Chinese redbud extract and the Yunnan white drug powder both have obvious hemostatic effect on the bleeding of the local wound surface of the rabbit liver and have obvious hemostatic effect;

fifth, conclusion

The application of the Chinese redbud extract as an active ingredient with the effects of clearing heat and cooling blood in preparing oral care products is completely feasible, and the toothpaste containing the Chinese redbud extract has the effects of clearing heat, removing fire, preventing gingival bleeding and the like.

Claims

1. The application of the bauhinia extract in oral care products is characterized in that: the bauhinia extract is added into oral care products as an active ingredient for clearing heat, cooling blood, inhibiting bacteria, stopping bleeding and preventing gingival bleeding.

2. Use according to claim 1, characterized in that: the mass percentage of the bauhinia extract in the oral care product is 0.01-2.0%.

3. Use according to claim 1, characterized in that:

the preparation process of the bauhinia extract comprises the following steps:

4. Use according to claim 3, characterized in that:

the weight of ethanol is 2 times of that of the Chinese redbud flower, and the concentration is 75 vol.%.

5. Use according to any one of claims 1 to 4, characterized in that:

the content of total flavone in the Cercis chinensis flower extract is 10-20 wt%.

6. An oral care product having a gingival bleeding prevention effect, characterized in that: the oral care product contains 0.01-2.0% of the bauhinia extract.

7. The oral care implement of claim 6, wherein: the oral care product is toothpaste.

8. The oral care implement of claim 6, wherein: the oral care product is mouthwash.