CN112494349A

CN112494349A - Oral care composition with anti-sensitivity, gum repairing and antibacterial effects

Info

Publication number: CN112494349A
Application number: CN202011502342.5A
Authority: CN
Inventors: 朱礼飞; 许可; 张腾飞; 李春波
Original assignee: Zhejiang Yongyu Biotechnology Co ltd
Current assignee: Zhejiang Yongyu Biotechnology Co ltd
Priority date: 2020-12-17
Filing date: 2020-12-17
Publication date: 2021-03-16

Abstract

The invention relates to an oral care composition with anti-sensitivity, gingival repair and antibacterial effects, which is prepared from the following raw materials in percentage by weight: 0.2-2% of hesperidin, 0.1-1% of magnolol, 0.5-4% of expanded perlite, 0.2-0.8% of potassium oxalate, 0.2-0.8% of strontium lactate, 1-4% of calcium silicate, 0.02-0.1% of sodium monofluorophosphate, 1-3% of flavoring agent, 1-3% of coloring agent, 0.2-1% of preservative and the balance of orally acceptable carriers. The oral care composition prepared by the invention has a remarkable inhibiting effect on common pathogenic bacteria of teeth, can play a good treatment effect in terms of gum repair, bacteriostasis and inflammation diminishing, and can also effectively reduce the area of dentinal tubules and reduce or avoid liquid flow in the dentinal tubules, thereby reducing the sensitivity of teeth.

Description

Oral care composition with anti-sensitivity, gum repairing and antibacterial effects

Technical Field

The invention relates to the technical field of oral care products, in particular to an oral care composition with anti-sensitivity, gingival repair and antibacterial effects.

Background

The oral cavity is a warm and moist irregular cavity which is directly communicated with the outside, and the temperature, the humidity and the food residues of the oral cavity are suitable for the growth and the propagation of microorganisms, so that the oral cavity is unsanitary, and even oral diseases such as oral local inflammation, ulcer and the like occur. Oral care is realized by selecting proper oral care products by adopting a certain method so as to achieve the purposes of cleaning the oral cavity, removing bacterial plaque, preventing and treating oral inflammation and the like.

The normal saline is the traditional nursing liquid in oral cavity nursing, has proved the function of cleaning oral cavity, has no peculiar smell, and has low price, easy acceptance by patients and wider clinical application, but the normal saline has no bactericidal function. At present, the oral care products have a wide variety of application ranges, and as for the components, the oral care products comprise antibiotics, surfactants, traditional Chinese medicines, minority herbs and the like. The western medicine oral care product is a chlorhexidine solution which has the widest application range at present, and chlorhexidine has a broad-spectrum antibacterial effect, can effectively remove dental plaque for 12 hours, and has strong affinity to oral mucosa and tooth surfaces. The traditional Chinese medicine oral care products mainly prevent oral infection, prevent and treat halitosis, prevent oral complications such as oral ulcer, parotitis and the like, such as honeysuckle flower dried herbal water, astragalus membranaceus water, isatis root liquid, honeysuckle flower mouthwash, radix zanthoxyli mouthwash and the like, and have certain effects of keeping the oral cavity clean and preventing oral infection.

The main effects of the currently marketed oral care products are: cleaning, inhibiting bacteria, resisting halitosis, preventing dental caries, and resisting sensitivity. The oral care evaluation indexes include antibacterial property, oral cleanliness, oral pH value, oral odor, oral care complications and the like. The comfort degree evaluation of the patient is carried out according to the individual subjective judgment of the patient, if the patient has dry and sore throat, fresh oral cavity, mouth feel and the like, the comfort degree is divided into comfortable, comfortable and uncomfortable degrees, and the satisfaction degree of oral care is also taken as an evaluation index. In addition, the average thickness of the dental plaque biofilm, the dental plaque index of the teeth, and the like can also be used as evaluation indexes. Although the oral care products sold on the market have the effects of cleaning, whitening, breath freshening, anti-inflammation, gingival bleeding prevention and the like, most of the products have the problems of short onset time, insufficient functional components, unreasonable formula, complex preparation process and the like, and some of the products can even damage enamel and tooth surface, stimulate oral mucosa and are not beneficial to oral health after long-term use.

Disclosure of Invention

The invention aims to provide an oral care composition which simultaneously has multiple effects of anti-sensitivity, gum repair, bacteriostasis and the like, can effectively repair oral problems of sensitive teeth, gum swelling and pain, decayed teeth, halitosis and the like, maintains oral health and meets the daily oral cleaning requirement of people.

In order to achieve the purpose, the invention adopts the technical scheme that:

an oral care composition with anti-sensitivity, gingival repair and antibacterial effects is prepared from the following raw materials in percentage by weight: 0.2-2% of hesperidin, 0.1-1% of magnolol, 0.5-4% of expanded perlite, 0.2-0.8% of potassium oxalate, 0.2-0.8% of strontium lactate, 1-4% of calcium silicate, 0.02-0.1% of sodium monofluorophosphate, 1-3% of flavoring agent, 1-3% of coloring agent, 0.2-1% of preservative and the balance of orally acceptable carriers.

Preferably, the oral care composition is prepared from the following raw materials in percentage by weight: 0.5-1.5% of hesperidin, 0.2-0.8% of magnolol, 1-3% of expanded perlite, 0.4-0.6% of potassium oxalate, 0.4-0.6% of strontium lactate, 1-3% of calcium silicate, 0.04-0.08% of sodium monofluorophosphate, 1.5-2.5% of flavoring agent, 1.5-2.5% of coloring agent, 0.4-0.8% of preservative and the balance of orally acceptable carriers.

Preferably, the oral care composition is prepared from the following raw materials in percentage by weight: 1% of hesperidin, 0.5% of magnolol, 2% of expanded perlite, 0.5% of potassium oxalate, 0.5% of strontium lactate, 2% of calcium silicate, 0.06% of sodium monofluorophosphate, 2% of flavoring agent, 2% of coloring agent, 0.5% of preservative and the balance of orally acceptable carriers.

Preferably, the particle size of the expanded perlite is in the range of 10-30 microns.

Preferably, the calcium silicate has a particle size in the range of 2 to 10 microns.

Preferably, the flavoring agent is selected from menthol, borneol, xylitol or stevioside.

Preferably, the colorant is selected from titanium dioxide, ultramarine blue, iron oxide red, phthalocyanine green, lemon yellow or pearl powder.

Preferably, the preservative is selected from phenoxyethanol, potassium sorbate, benzyl alcohol or triclosan.

Preferably, the oral care composition is selected from the group consisting of mouthwashes, toothpastes, oral sprays, chewable tablets or chewing gums.

Further, the invention also provides application of the oral care composition in preparing oral care products.

Hesperidin (glycoside) is a flavonoid substance, has a dihydroflavodoxin structure, and is weakly acidic. The pure product is white needle crystal, and is the main component of vitamin P. Hesperidin has various biological characteristics, and modern researches find that: hesperidin has effects of resisting oxidation and cancer, preventing mildew and allergy, lowering blood pressure, inhibiting oral cancer and esophageal cancer, maintaining osmotic pressure, enhancing capillary toughness, and reducing cholesterol. Relevant studies have shown that: the hesperidin has broad-spectrum bacteriostatic action on common food-polluting bacteria, and has obvious inhibitory action on bacillus subtilis, salmonella typhimurium, shigella flexneri, streptococcus hemolyticus, vibrio cholerae and the like. In addition, hesperidin can be used in oral hygiene products for inhibiting dental plaque formation and simultaneously reducing halitosis. The chemical structure of hesperidin is as follows:

the magnolol is derived from wood

An active substance extracted from dried dry bark, branch bark and root bark of Magnolia officinalis of Orchidaceae (Magnolia officinalis) has antibacterial, antiinflammatory, antitumor, endomycin resisting, muscle relaxing, and acute inflammatory pain relieving effects. In the aspect of antibacterial action, magnolol has obvious antibacterial activity on gram-positive bacteria, acid-resistant bacteria and filamentous fungi, has more obvious antibacterial action on streptococcus mutans, and has the strongest inhibition effect on staphylococcus. The traditional Chinese medicine composition is mainly used for eliminating chest and abdomen fullness and stuffiness, calming central nerves, relaxing muscles of athletes, resisting fungi, resisting ulcers and the like in clinic. Magnolol has strong anti-dental caries effect, and is mainly effective on Streptococcus mutans in oral cavity. The chemical structure of magnolol is as follows:

perlite is a volcano-ejected acidic vitreous lava, which is rapidly cooled to form a vitreous rock, which is named because it has a pearl fracture structure. The expanded perlite is a non-toxic, tasteless, non-inflammable, non-corrosive, acid-base resistant, heat insulating, sound insulating and heat insulating product with honeycomb structure inside, and is a white or light-colored high-quality heat insulating material prepared by crushing acid volcano vitreous lava (perlite), sieving to a certain particle size, preheating, and sintering at 14000 ℃ for a long time. The expanded perlite used as the grinding agent can effectively remove tartar and dental plaque without damaging enamel, and has the effects of whitening and brightening teeth.

Strontium is an important microelement essential to human body, is important for human body, just like steel bar cement for building human body, and is an important component of human skeleton and tooth. Lack of strontium easily causes fracture difficult healing, white hair, dental caries and senile osteoporosis. Strontium also participates in blood circulation, and can improve absorption and metabolism of sodium salt in vivo, and prevent hypertension and cardiovascular diseases. Strontium salts (e.g., strontium lactate) are often used in oral care compositions to provide anti-sensitivity to teeth because they can occlude dentinal tubules and prevent fluid flow in the tubules.

After the calcium silicate biomaterial is subjected to self-curing reaction, hydroxyapatite precipitate is generated on the surface of the material and permeates into the dentin tubules to form chemical bonding with dentin, so that the calcium silicate biomaterial has better root canal sealing performance. The calcium silicate has good biocompatibility and also has strong anti-inflammatory capability. The calcium silicate biomaterial has low solubility and long calcium ion continuous release time, can enhance the alkalization effect of the surrounding environment and can induce the formation of apatite precipitates.

Compared with the prior art, the invention has the beneficial effects that: (1) the oral care composition prepared by the invention has obvious inhibition effect on common pathogenic bacteria of teeth, namely porphyromonas gingivalis, staphylococcus aureus, streptococcus mutans, pseudomonas aeruginosa and candida albicans. When the active ingredients hesperidin and magnolol are used in a specific ratio (2:1) in a matching way, a synergistic effect can be generated in the aspect of inhibiting the growth of tooth pathogenic bacteria, and the antibacterial efficiency can be obviously improved by using the hesperidin and magnolol in a matching way. The tooth pathogenic bacteria play an important role in the occurrence and development of dental caries, gingivitis and periodontitis, so that the oral care composition disclosed by the invention can play a good treatment effect in the aspects of gingival repair, bacteriostasis and inflammation diminishing. (2) The oral care composition prepared by the invention can effectively improve the accumulation of crystal substances in the dentinal tubules, reduce the area of the dentinal tubules, further reduce or avoid the liquid flow in the dentinal tubules, and further reduce the tooth sensitivity. When the anti-sensitivity components potassium oxalate, strontium lactate and calcium silicate are matched and used in a specific ratio (1:1:4), the area of dentinal tubules can be obviously reduced, namely, a synergistic effect can be generated in the aspect of reducing the sensitivity of teeth.

Detailed Description

Terms used in the present invention have generally meanings as commonly understood by one of ordinary skill in the art, unless otherwise specified. The present invention will be described in further detail with reference to the following data in conjunction with specific examples. The following examples are intended to illustrate the invention and are not intended to limit the scope of the invention in any way.

Example 1

An oral care composition, which is prepared from the following raw materials in percentage by weight: 0.5% of hesperidin, 0.8% of magnolol, 1% of expanded perlite, 0.2% of potassium oxalate, 0.6% of strontium lactate, 1% of calcium silicate, 0.02% of sodium monofluorophosphate, 1% of menthol, 3% of ultramarine blue, 0.2% of triclosan and the balance of an orally acceptable carrier.

Example 2

An oral care composition, which is prepared from the following raw materials in percentage by weight: 1.5% of hesperidin, 0.2% of magnolol, 3% of expanded perlite, 0.6% of potassium oxalate, 0.2% of strontium lactate, 4% of calcium silicate, 0.08% of sodium monofluorophosphate, 3% of xylitol, 1% of iron oxide red, 1% of benzyl alcohol and the balance of an orally acceptable carrier.

Example 3

An oral care composition, which is prepared from the following raw materials in percentage by weight: 1% of hesperidin, 0.5% of magnolol, 2% of expanded perlite, 0.5% of potassium oxalate, 0.5% of strontium lactate, 2% of calcium silicate, 0.06% of sodium monofluorophosphate, 2% of borneol, 2% of titanium dioxide, 0.5% of phenoxyethanol and the balance of an orally acceptable carrier.

Comparative example 1

An oral care composition, which is prepared from the following raw materials in percentage by weight: 1.5% of hesperidin, 2% of expanded perlite, 0.5% of potassium oxalate, 0.5% of strontium lactate, 2% of calcium silicate, 0.06% of sodium monofluorophosphate, 2% of borneol, 2% of titanium dioxide, 0.5% of phenoxyethanol and the balance of an orally acceptable carrier.

Comparative example 2

An oral care composition, which is prepared from the following raw materials in percentage by weight: 1.5 percent of magnolol, 2 percent of expanded perlite, 0.5 percent of potassium oxalate, 0.5 percent of strontium lactate, 2 percent of calcium silicate, 0.06 percent of sodium monofluorophosphate, 2 percent of borneol, 2 percent of titanium pigment, 0.5 percent of phenoxyethanol and the balance of carriers acceptable for oral cavity.

Comparative example 3

An oral care composition, which is prepared from the following raw materials in percentage by weight: 1% of hesperidin, 0.5% of magnolol, 2% of expanded perlite, 3% of potassium oxalate, 0.06% of sodium monofluorophosphate, 2% of borneol, 2% of titanium dioxide, 0.5% of phenoxyethanol and the balance of an orally acceptable carrier.

Comparative example 4

An oral care composition, which is prepared from the following raw materials in percentage by weight: 1% of hesperidin, 0.5% of magnolol, 2% of expanded perlite, 3% of strontium lactate, 0.06% of sodium monofluorophosphate, 2% of borneol, 2% of titanium dioxide, 0.5% of phenoxyethanol and the balance of an orally acceptable carrier.

Comparative example 5

An oral care composition, which is prepared from the following raw materials in percentage by weight: 1% of hesperidin, 0.5% of magnolol, 2% of expanded perlite, 3% of calcium silicate, 0.06% of sodium monofluorophosphate, 2% of borneol, 2% of titanium dioxide, 0.5% of phenoxyethanol and the balance of an orally acceptable carrier.

The oral care compositions prepared in the above example 3 and comparative examples 1 to 5 were mixed with toothpaste usual excipients, respectively, and a toothpaste composition containing 50 wt% of the oral care composition was prepared according to a conventional technical means.

Antibacterial performance research of toothpaste composition

2g of the toothpaste compositions of the example 3 and the comparative examples 1 to 2 are accurately weighed by an electronic balance into a sterile empty plate, 10ml of sterile physiological saline is added, and the mixture is stirred and mixed by a sterile rod to prepare toothpaste stock solution with the concentration of 2 g/ml. According to the preparation method of the bacterial suspension in the technical Specification for disinfection, bacterial suspensions of tested strains of porphyromonas gingivalis, staphylococcus aureus, streptococcus mutans, pseudomonas aeruginosa and candida albicans are respectively prepared, so that the final concentration of the bacterial liquid is about 0.5 multiplied by 106 cfu/ml. 2ml of the bacterial suspension is sucked up, evenly spread in a plate culture medium, covered with a plate and dried for 5 minutes at room temperature. Taking the aseptic treated filter paper sheet by using tweezers, immersing the aseptic treated filter paper sheet into the toothpaste stock solution to enable the filter paper sheet to fully absorb the toothpaste stock solution, taking out the filter paper sheet for slightly draining, carefully sticking the filter paper sheet on the surfaces of the flat plates, sticking 3 sheets on each flat plate, slightly pressing the filter paper sheet by using the aseptic tweezers to enable the filter paper sheet to be tightly stuck on the surfaces of the flat plates, covering the flat plates, putting the flat plates into a constant-temperature incubator, and culturing for 24 hours at 37 ℃. After the culture is finished, the diameter (mm) of the inhibition zone is accurately measured by a vernier caliper. The results of the experiment are shown in table 1.

TABLE 1 antibacterial action of toothpaste compositions (n ═ 3, mm)

As can be seen from the experimental results in table 1, the toothpaste composition prepared from the oral care composition of example 3 has significant inhibitory effects on 5 common pathogenic strains of teeth, namely, porphyromonas gingivalis, staphylococcus aureus, streptococcus mutans, pseudomonas aeruginosa and candida albicans. Compared with the comparative examples 1-2, the experiment results show that the synergistic effect on inhibiting the growth of pathogenic bacteria of teeth can be generated when the active ingredients hesperidin and magnolol are used in a specific ratio (2:1), and the antibacterial efficiency can be obviously improved when the hesperidin and magnolol are used in a compatible manner. The tooth pathogenic bacteria play an important role in the occurrence and development of dental caries, gingivitis and periodontitis, so that the oral care composition disclosed by the invention can play a good treatment effect in the aspects of gingival repair, bacteriostasis and inflammation diminishing.

Anti-sensitivity performance study of toothpaste composition

50 healthy and intact non-carious isolated premolars were selected, and the dental calculus and plaque were cleaned and then stored in artificial saliva. The sample is polished by a high-speed carborundum needle to expose the superficial dentin on the surface, and carborundum sand is used under the condition of water perfusionGrinding paper to 3mm²Ultrasonic cleaning for 10min for later use. The tooth samples were randomly divided into 5 groups, each containing 10 teeth. The toothpaste composition containing the oral care compositions of example 3 and comparative examples 3 to 5 was uniformly applied to the dentin surface, gently brushed, rinsed with distilled water, and dried. The blank control group was not treated with toothpaste. The test specimens were brushed for 3min for each application, at 0, 12, and 24h, respectively, and placed in artificial saliva (1 change every 12 hours) for the interim period. The tooth samples were placed in a 37 ℃ incubator to simulate a normal oral environment. Obtaining real-time images by using an atomic force microscope on corresponding time nodes, and measuring and calculating the area (mum) of the dentinal tubule by analysis software²) And relative area (i.e., the percentage of tubule area over the entire picture area,%). The results of the experiment are shown in table 2.

TABLE 2 antiallergic action of toothpaste compositions

As can be seen from the experimental results in table 2, the toothpaste composition prepared from the oral care composition of example 3 is applied to the dentin surface for several times in a time-sharing manner, so as to effectively increase the accumulation of crystalline substances and reduce the area of dentinal tubules, which indicates that the toothpaste composition can close the dentinal tubules, thereby reducing or preventing the flow of liquid in the dentinal tubules, and thus reducing the sensitivity of teeth. Comparing example 3 with comparative examples 3-5, it can be seen that the use of potassium oxalate, strontium lactate and calcium silicate in a specific ratio (1:1:4) in combination can significantly reduce the area of dentinal tubules, i.e. can produce a synergistic effect in reducing the sensitivity of teeth.

The above is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, several modifications or equivalents may be made to the technical solution without departing from the principle of the present invention, and these modifications or equivalents should also be regarded as the protection scope of the present invention.

Claims

1. An oral care composition with anti-sensitivity, gingival repair and antibacterial effects, which is characterized by being prepared from the following raw materials in percentage by weight: 0.2-2% of hesperidin, 0.1-1% of magnolol, 0.5-4% of expanded perlite, 0.2-0.8% of potassium oxalate, 0.2-0.8% of strontium lactate, 1-4% of calcium silicate, 0.02-0.1% of sodium monofluorophosphate, 1-3% of flavoring agent, 1-3% of coloring agent, 0.2-1% of preservative and the balance of orally acceptable carriers.

2. The oral care composition according to claim 1, wherein the oral care composition is prepared from raw materials comprising, by weight: 0.5-1.5% of hesperidin, 0.2-0.8% of magnolol, 1-3% of expanded perlite, 0.4-0.6% of potassium oxalate, 0.4-0.6% of strontium lactate, 1-3% of calcium silicate, 0.04-0.08% of sodium monofluorophosphate, 1.5-2.5% of flavoring agent, 1.5-2.5% of coloring agent, 0.4-0.8% of preservative and the balance of orally acceptable carriers.

3. The oral care composition according to claim 2, wherein the oral care composition is prepared from raw materials comprising, by weight: 1% of hesperidin, 0.5% of magnolol, 2% of expanded perlite, 0.5% of potassium oxalate, 0.5% of strontium lactate, 2% of calcium silicate, 0.06% of sodium monofluorophosphate, 2% of flavoring agent, 2% of coloring agent, 0.5% of preservative and the balance of orally acceptable carriers.

4. The oral care composition of any one of claims 1 to 3, wherein the expanded perlite has a particle size in the range of 10 to 30 microns.

5. The oral care composition according to any one of claims 1 to 3, wherein the calcium silicate has a particle size in the range of from 2 to 10 microns.

6. An oral care composition according to any one of claims 1 to 3 wherein the flavouring agent is selected from menthol, borneol, xylitol or stevioside.

7. An oral care composition according to any one of claims 1 to 3 wherein the colorant is selected from titanium dioxide, ultramarine blue, iron oxide red, phthalocyanine green, lemon yellow or pearl powder.

8. The oral care composition of any one of claims 1 to 3, wherein the preservative is selected from phenoxyethanol, potassium sorbate, benzyl alcohol, or triclosan.

9. The oral care composition according to any one of claims 1 to 3, wherein the oral care composition is selected from the group consisting of mouthwashes, toothpastes, oral sprays, chewable tablets, and chewing gums.

10. Use of an oral care composition according to any one of claims 1 to 9 in the manufacture of an oral care product.