CN113875499A - Method for preparing edible fungus culture medium by utilizing wet garbage to perform rapid fermentation - Google Patents
Method for preparing edible fungus culture medium by utilizing wet garbage to perform rapid fermentation Download PDFInfo
- Publication number
- CN113875499A CN113875499A CN202111331409.8A CN202111331409A CN113875499A CN 113875499 A CN113875499 A CN 113875499A CN 202111331409 A CN202111331409 A CN 202111331409A CN 113875499 A CN113875499 A CN 113875499A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- wet garbage
- culture medium
- edible fungus
- steps
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000855 fermentation Methods 0.000 title claims abstract description 98
- 230000004151 fermentation Effects 0.000 title claims abstract description 98
- 241000233866 Fungi Species 0.000 title claims abstract description 70
- 239000010813 municipal solid waste Substances 0.000 title claims abstract description 62
- 238000000034 method Methods 0.000 title claims abstract description 36
- 239000001963 growth medium Substances 0.000 title claims abstract description 22
- 239000000463 material Substances 0.000 claims abstract description 66
- 238000003756 stirring Methods 0.000 claims abstract description 36
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 26
- 230000001954 sterilising effect Effects 0.000 claims abstract description 19
- ODINCKMPIJJUCX-UHFFFAOYSA-N Calcium oxide Chemical compound [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 claims abstract description 14
- 240000001462 Pleurotus ostreatus Species 0.000 claims abstract description 13
- 235000001603 Pleurotus ostreatus Nutrition 0.000 claims abstract description 13
- QWPPOHNGKGFGJK-UHFFFAOYSA-N hypochlorous acid Chemical compound ClO QWPPOHNGKGFGJK-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 9
- 238000002156 mixing Methods 0.000 claims abstract description 8
- 235000012255 calcium oxide Nutrition 0.000 claims abstract description 7
- 239000000292 calcium oxide Substances 0.000 claims abstract description 7
- 238000001816 cooling Methods 0.000 claims abstract description 7
- 238000011081 inoculation Methods 0.000 claims abstract description 7
- 238000005507 spraying Methods 0.000 claims abstract description 7
- 239000000725 suspension Substances 0.000 claims abstract description 7
- 235000016709 nutrition Nutrition 0.000 claims description 18
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 17
- 235000010855 food raising agent Nutrition 0.000 claims description 14
- 244000063299 Bacillus subtilis Species 0.000 claims description 13
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 13
- 241000195940 Bryophyta Species 0.000 claims description 13
- 241000228212 Aspergillus Species 0.000 claims description 12
- 241000132152 Polymyxa Species 0.000 claims description 12
- 230000035764 nutrition Effects 0.000 claims description 12
- 241000186146 Brevibacterium Species 0.000 claims description 9
- 239000012634 fragment Substances 0.000 claims description 9
- 229920000742 Cotton Polymers 0.000 claims description 7
- 240000008042 Zea mays Species 0.000 claims description 7
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 claims description 7
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 7
- 235000005822 corn Nutrition 0.000 claims description 7
- 230000018044 dehydration Effects 0.000 claims description 7
- 238000006297 dehydration reaction Methods 0.000 claims description 7
- 238000001125 extrusion Methods 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 235000001674 Agaricus brunnescens Nutrition 0.000 claims 1
- 238000000354 decomposition reaction Methods 0.000 abstract description 10
- 235000015097 nutrients Nutrition 0.000 abstract description 8
- 244000005700 microbiome Species 0.000 abstract description 6
- 238000010438 heat treatment Methods 0.000 abstract description 4
- 230000008569 process Effects 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 239000002068 microbial inoculum Substances 0.000 description 3
- 230000015556 catabolic process Effects 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000005265 energy consumption Methods 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 241000193755 Bacillus cereus Species 0.000 description 1
- 239000002028 Biomass Substances 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 241000006384 Jeotgalibacillus marinus Species 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 238000003915 air pollution Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000010791 domestic waste Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000010794 food waste Substances 0.000 description 1
- 235000021190 leftovers Nutrition 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 239000007858 starting material Substances 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 235000013311 vegetables Nutrition 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/20—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation using specific microorganisms or substances, e.g. enzymes, for activating or stimulating the treatment
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F17/00—Preparation of fertilisers characterised by biological or biochemical treatment steps, e.g. composting or fermentation
- C05F17/80—Separation, elimination or disposal of harmful substances during the treatment
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Abstract
The invention belongs to a method for preparing an edible fungus culture medium by utilizing wet garbage to rapidly ferment, which comprises the following steps: step 1: crushing, extruding and dehydrating the wet garbage, putting the wet garbage into a fermentation stirring tank, putting a biological fermentation agent into the fermentation stirring tank, and stirring and fermenting; step 2: mixing the rapid fermentation residue with a nutrient support material to form a mixed material; and step 3: spraying hypochlorous acid solution on the mixed material for disinfection; and 4, step 4: adding a quicklime suspension into the sterilized mixed material, and adjusting the pH value; and 5: bagging the material with the adjusted pH value; step 6: placing the bagged fungus bags in an autoclave for sterilization; and 7: ventilating and cooling the sterilized fungus bags, and completing the inoculation of oyster mushroom strains; the method has the characteristics of short fermentation time, no need of external heating, high decomposition rate of wet garbage and capability of preparing the edible fungus culture medium within 24 hours by adopting high-temperature aerobic microorganism for fermentation.
Description
Technical Field
The invention belongs to the technical field of wet garbage recycling, and particularly relates to a method for rapidly fermenting and blending an edible fungus culture medium by using wet garbage.
Background
With the continuous progress of living conditions of people, wet garbage generated in life is increased year by year, and how to treat the wet garbage is researched and developed based on the enterprise, wherein the mature treatment mode is that the wet garbage is rapidly fermented in an aerobic mode and is gradually accepted by the society; but the treatment of the residual products after rapid fermentation becomes a new problem; enterprises can solve the air pollution of wet garbage and the generation of pathogenic microorganisms by rapidly fermenting the wet garbage, and reduce the occupied land of garbage landfill and stacking; however, if the residual products after the rapid fermentation need to be prepared into organic fertilizers, long-time stacking fermentation is still needed, a certain field needs to be occupied in the process, a certain environmental temperature is kept, the stacking time is long, and the economic benefit is limited.
Disclosure of Invention
The invention aims to overcome the defects in the prior art, and provides a method for preparing an edible fungus culture medium by utilizing wet garbage for rapid fermentation, which adopts high-temperature aerobic microorganism for fermentation, has short fermentation time, does not need external heating, has high decomposition rate on the wet garbage and can realize the preparation of the edible fungus culture medium within 24 hours.
The purpose of the invention is realized as follows:
a method for preparing an edible fungus culture medium by utilizing wet garbage for rapid fermentation comprises the following steps:
step 1: crushing, squeezing and dehydrating the wet garbage, then placing the wet garbage into a fermentation stirring tank, putting an inoculated biological fermentation agent into the fermentation stirring tank, and stirring and fermenting to obtain a quick fermentation residue;
step 2: mixing the rapid fermentation residue obtained in the step 1 with a nutritional support material to prepare a mixed material;
and step 3: spraying hypochlorous acid solution with the concentration of 1% on the mixed material for disinfection;
and 4, step 4: adding a quicklime suspension with the concentration of 1% into the mixed material sterilized in the step 3 to enable the pH value to reach 8-8.5;
and 5: bagging the material with the adjusted pH value;
step 6: placing the bagged fungus bags in an autoclave for sterilization;
and 7: ventilating and cooling the sterilized fungus bags in the step 6, and placing the cooled fungus bags on a sterile table to complete inoculation of oyster mushroom strains;
and 8: placing the inoculated fungus bags in a dark place with the ambient temperature of 25-27 ℃ and the ambient humidity of 80-85%, and observing the growth of edible fungus hyphae by naked eyes after 12 hours, wherein the hyphae grow over the fungus bags after 5-7 days;
and step 9: and (3) placing the fungus bag full of hypha in a ventilated place with the ambient temperature of 25-27 ℃ and the ambient humidity of 85-90% until the oyster mushroom grows.
Preferably, the moisture content of the wet garbage after extrusion dehydration in the step 1 is 65-75%.
Preferably, the biological leavening agent in the step 1 comprises bacillus subtilis, bryophyte, bacillus vegicus, brevibacterium limosum, polymyxa and aspergillus, and the ratio of the bacillus subtilis, the bryophyte, the bacillus vegicus, the bacillus limosum, the polymyxa and the aspergillus is 3.5:2:1.5:1:1: 1.
Preferably, the adding amount of the biological leaven is 100 g/t.
Preferably, the stirring fermentation in the step 1 is performed at a stirring rotation speed of 100-120 r/min for 12-14 hours in an environment with a fermentation temperature of 60-75 ℃; the water content of the rapid fermentation residue is 8-12%.
Preferably, in the step 2, the nutrition supporting material is cotton seed hulls or corn cob fragments, and the volume ratio of the quick fermentation residues to the nutrition supporting material is as follows: 5: 1.
preferably, the corncob fragments refer to corncobs crushed to 15 meshes.
Preferably, the volume ratio of the mixed material to the hypochlorous acid solution in the step 3 is as follows: 5: 1.
preferably, in the step 6, the temperature in the high-pressure sterilization pot is 120-125 ℃, and the pressure is as follows: 0.9 to 1.1kgf/cm2The sterilization time was 2 hours.
The method adopts high-temperature aerobic microorganism for fermentation, has short fermentation time, does not need external heating, has high decomposition rate of wet garbage, and can realize the characteristic of preparing the edible fungus culture medium within 24 hours.
Detailed Description
In order to more clearly understand the technical features, objects, and effects of the present invention, specific embodiments of the present invention will now be described.
The invention relates to a method for preparing an edible fungus culture medium by utilizing wet garbage to rapidly ferment, which comprises the following steps:
step 1: crushing, squeezing and dehydrating the wet garbage, then placing the wet garbage into a fermentation stirring tank, putting an inoculated biological fermentation agent into the fermentation stirring tank, and stirring and fermenting to obtain a quick fermentation residue;
step 2: mixing the rapid fermentation residue obtained in the step 1 with a nutritional support material to prepare a mixed material;
and step 3: spraying hypochlorous acid solution with the concentration of 1% on the mixed material for disinfection;
and 4, step 4: adding a quicklime suspension with the concentration of 1% into the mixed material sterilized in the step 3 to enable the pH value to reach 8-8.5;
and 5: bagging the material with the adjusted pH value;
step 6: placing the bagged fungus bags in an autoclave for sterilization;
and 7: ventilating and cooling the sterilized fungus bags in the step 6, and placing the cooled fungus bags on a sterile table to complete inoculation of oyster mushroom strains;
and 8: placing the inoculated fungus bags in a dark place with the ambient temperature of 25-27 ℃ and the ambient humidity of 80-85%, and observing the growth of edible fungus hyphae by naked eyes after 12 hours, wherein the hyphae grow over the fungus bags after 5-7 days;
and step 9: and (3) placing the fungus bag full of hypha in a ventilated place with the ambient temperature of 25-27 ℃ and the ambient humidity of 85-90% until the oyster mushroom grows.
The wet garbage in the invention refers to all wet garbage including kitchen garbage, instant food waste, leftovers, expired food, melon peel and fruit stone, green flowers, traditional Chinese medicine dregs and other perishable biomass domestic wastes; the wet garbage is crushed, extruded and dehydrated to provide a good growth environment for the inoculated biological leavening agent, and the microbial inoculum mainly comprises an aerobic microbial inoculum so as to achieve the purpose of rapid fermentation; after the fermentation is finished, the mixed nutrient supporting material supports the particles with the undersize particle size after the fermentation so as to achieve the purpose of ensuring the rapid growth of the microorganisms, and on the basis, nutrients required by the growth of the microorganisms can be provided for the microorganisms.
Further, the moisture content of the wet garbage after extrusion dehydration in the step 1 is 65-75%. The foundation can be laid for the subsequent fermentation through the extrusion dehydration of the wet garbage.
Further, the biological leavening agent in the step 1 comprises bacillus subtilis, bryophyte, bacillus vegicus, brevibacterium limosum, polymyxa and aspergillus, and the ratio of the bacillus subtilis, the bryophyte, the bacillus vegicus, the bacillus limosum, the polymyxa and the aspergillus is 3.5:2:1.5:1:1: 1. The biological leaven is an aerobic leaven and has the characteristics of high temperature resistance and low energy consumption, wherein the bacillus marinus and the brevibacterium limonum can grow and breed under the normal temperature condition and finish the decomposition of partial garbage, heat can be generated in the decomposition process to raise the temperature of materials (the temperature of the materials can reach 45 ℃ at most), and the bacillus subtilis, the bryophyte, the polymyxa bacteria and the aspergillus grow and breed to decompose wet garbage when the temperature of the materials reaches 40 ℃ and release heat (the temperature of the materials can reach 65 ℃ at most). Based on the working process, the biological leavening agent is mainly characterized in that garbage is decomposed in sections, bacillus brassicae and brevibacterium limonum are used for decomposing at medium and low temperatures, and other high-temperature resistant strains except the strains are used for decomposing at high temperatures; therefore, the nutrients which are not easy to be absorbed by the edible fungi in the wet garbage are decomposed, and the nutrients are decomposed into micromolecular proteins, so that the aims of improving the decomposition efficiency, facilitating the growth and absorption of the edible fungi and reducing the wet garbage are fulfilled; in addition, it should be noted that the biological fermentation agent in the present invention does not need external heating or a certain temperature maintenance because of its self-heat generation during the fermentation process.
Further, the adding amount of the biological leavening agent is 100 g/t.
Further, the stirring fermentation in the step 1 is to stir at a rotating speed of 100-120 r/min for 12-14 hours in an environment with a fermentation temperature of 60-75 ℃; the water content of the rapid fermentation residue is 8-12%. According to the invention, the wet garbage is fermented by adopting a fermentation stirring tank in a stirring and fermenting manner according to the characteristics of the biological fermentation agent, heat is generated in the fermentation process, the temperature of the material can be uniformly decomposed integrally and in stages by stirring, the aerobic requirement of a microbial inoculum in the decomposition process can be met, and the decomposition rate of the wet garbage can be further improved. Compared with conventional fermentation, the method has the characteristics of short fermentation time period, low energy consumption and high decomposition rate.
Further, the nutrition supporting material in the step 2 is cotton seed hulls or corn cob fragments, and the volume ratio of the quick fermentation residues to the nutrition supporting material is as follows: 5: 1. the biological leavening agent adopted in the invention is an aerobic leavening agent, and in the actual decomposition process, after the leavening is decomposed due to the action of biological bacteria, the granularity is too small, and the air in the material is thin and is not beneficial to the growth of the bacteria after being extruded, and based on the invention, the selected nutrition supporting material is cotton seed hulls or corn cob fragments, the material not only can provide nutrients for the bacteria, but also can provide support for the material, so that a small amount of gaps are reserved in the material for air circulation, and the aim of fluffy and air-permeable material is fulfilled.
Further, the corncob fragments refer to corncobs crushed to 15 meshes.
Further, the volume ratio of the mixed material to the hypochlorous acid solution in the step 3 is as follows: 5: 1.
further, the temperature in the high-pressure sterilization pot in the step 6 is 120-125 ℃, and the pressure is as follows: 0.9 to 1.1kgf/cm2The sterilization time was 2 hours.
The present invention will now be further illustrated with reference to examples in order to explain the present invention in more detail. The specific embodiment is as follows:
example 1
A method for preparing an edible fungus culture medium by utilizing wet garbage for rapid fermentation comprises the following steps:
step 1: crushing, squeezing and dehydrating the wet garbage, then placing the wet garbage into a fermentation stirring tank, putting an inoculated biological fermentation agent into the fermentation stirring tank, and stirring and fermenting to obtain a quick fermentation residue;
step 2: mixing the rapid fermentation residue obtained in the step 1 with a nutritional support material to prepare a mixed material;
and step 3: spraying hypochlorous acid solution with the concentration of 1% on the mixed material for disinfection;
and 4, step 4: adding a quicklime suspension with the concentration of 1% into the mixed material sterilized in the step 3 to enable the pH value to reach 8-8.5;
and 5: bagging the material with the adjusted pH value;
step 6: placing the bagged fungus bags in an autoclave for sterilization;
and 7: ventilating and cooling the sterilized fungus bags in the step 6, and placing the cooled fungus bags on a sterile table to complete inoculation of oyster mushroom strains;
and 8: placing the inoculated fungus bag in a dark place with the ambient temperature of 25 ℃ and the ambient humidity of 80%, and observing the growth of edible fungus hyphae with naked eyes after 12 hours, wherein the fungus bag is full of hyphae after 7 days;
and step 9: and (3) placing the fungus bag full of hypha in a ventilated place with the ambient temperature of 25 ℃ and the ambient humidity of 85% until the oyster mushroom grows.
The moisture content of the wet garbage after extrusion dehydration in the step 1 is 65%. The biological leavening agent in the step 1 comprises bacillus subtilis, bryophyte, bacillus vegicus, brevibacterium limosum, polymyxa and aspergillus, and the ratio of the bacillus subtilis, the bryophyte, the bacillus vegicus, the bacillus limosum, the polymyxa and the aspergillus is 3.5:2:1.5:1: 1. The adding amount of the biological leavening agent is 100 g/t. The stirring fermentation in the step 1 refers to stirring rotation speed of 100r/min for 12 hours at the fermentation temperature of 60 ℃; the rapid fermentation residue had a moisture content of 12%. The nutrition supporting material in the step 2 is cotton seed hulls or broken corn cob blocks, and residues are quickly fermentedThe volume ratio of the nutrient to the nutrient supporting material is as follows: 5: 1. the corncob fragments refer to corncobs crushed to 15 meshes. The volume ratio of the mixed material to the hypochlorous acid solution in the step 3 is as follows: 5: 1. the temperature in the high-pressure sterilization pot in the step 6 is 120 ℃, and the pressure is as follows: 0.9kgf/cm2The sterilization time was 2 hours.
Example 2
A method for preparing an edible fungus culture medium by utilizing wet garbage for rapid fermentation comprises the following steps:
step 1: crushing, squeezing and dehydrating the wet garbage, then placing the wet garbage into a fermentation stirring tank, putting an inoculated biological fermentation agent into the fermentation stirring tank, and stirring and fermenting to obtain a quick fermentation residue;
step 2: mixing the rapid fermentation residue obtained in the step 1 with a nutritional support material to prepare a mixed material;
and step 3: spraying hypochlorous acid solution with the concentration of 1% on the mixed material for disinfection;
and 4, step 4: adding a quicklime suspension with the concentration of 1% into the mixed material sterilized in the step 3 to enable the pH value to reach 8-8.5;
and 5: bagging the material with the adjusted pH value;
step 6: placing the bagged fungus bags in an autoclave for sterilization;
and 7: ventilating and cooling the sterilized fungus bags in the step 6, and placing the cooled fungus bags on a sterile table to complete inoculation of oyster mushroom strains;
and 8: placing the inoculated fungus bag in a shade with the ambient temperature of 27 ℃ and the ambient humidity of 85 percent, and observing the growth of edible fungus hyphae by naked eyes after 12 hours, wherein the fungus bag is full of hyphae after 5 days;
and step 9: and (3) placing the fungus bag full of hypha in a ventilated place with the ambient temperature of 27 ℃ and the ambient humidity of 90% until the oyster mushroom grows.
The moisture content of the wet garbage after extrusion dehydration in the step 1 is 75%. The biological leaven in the step 1 comprises bacillus subtilis, bryophyte, bacillus cereus, brevibacterium limosum and polymyxaThe ratio of the bacillus subtilis to the bryophyte to the vegetable bacillus, the brevibacterium limosum to the polymyxa to the aspergillus is 3.5:2:1.5:1:1: 1. The adding amount of the biological leavening agent is 100 g/t. The stirring fermentation in the step 1 refers to stirring rotation speed 1120r/min and fermentation time 14 hours under the environment of fermentation temperature 75 ℃; the rapid fermentation residue had a moisture content of 8%. In the step 2, the nutrition supporting material is cotton seed hulls or broken corn cobs, and the volume ratio of the quick fermentation residues to the nutrition supporting material is as follows: 5: 1. the corncob fragments refer to corncobs crushed to 15 meshes. The volume ratio of the mixed material to the hypochlorous acid solution in the step 3 is as follows: 5: 1. the temperature in the high-pressure sterilization pot in the step 6 is 125 ℃, and the pressure is as follows: 1.1kgf/cm2The sterilization time was 2 hours.
Example 3
A method for preparing an edible fungus culture medium by utilizing wet garbage for rapid fermentation comprises the following steps:
step 1: crushing, squeezing and dehydrating the wet garbage, then placing the wet garbage into a fermentation stirring tank, putting an inoculated biological fermentation agent into the fermentation stirring tank, and stirring and fermenting to obtain a quick fermentation residue;
step 2: mixing the rapid fermentation residue obtained in the step 1 with a nutritional support material to prepare a mixed material;
and step 3: spraying hypochlorous acid solution with the concentration of 1% on the mixed material for disinfection;
and 4, step 4: adding a quicklime suspension with the concentration of 1% into the mixed material sterilized in the step 3 to enable the pH value to reach 8-8.5;
and 5: bagging the material with the adjusted pH value;
step 6: placing the bagged fungus bags in an autoclave for sterilization;
and 7: ventilating and cooling the sterilized fungus bags in the step 6, and placing the cooled fungus bags on a sterile table to complete inoculation of oyster mushroom strains;
and 8: placing the inoculated fungus bag in a shade with the ambient temperature of 26 ℃ and the ambient humidity of 83 percent, and observing the growth of edible fungus hyphae by naked eyes after 12 hours, wherein the hyphae grow over the fungus bag after 6 days;
and step 9: and (3) placing the fungus bag full of hypha in a ventilated place with the ambient temperature of 26 ℃ and the ambient humidity of 87% until the oyster mushroom grows.
The moisture content of the wet garbage after extrusion dehydration in the step 1 is 70%. The biological leavening agent in the step 1 comprises bacillus subtilis, bryophyte, bacillus vegicus, brevibacterium limosum, polymyxa and aspergillus, and the ratio of the bacillus subtilis, the bryophyte, the bacillus vegicus, the bacillus limosum, the polymyxa and the aspergillus is 3.5:2:1.5:1: 1. The adding amount of the biological leavening agent is 100 g/t. The stirring fermentation in the step 1 refers to stirring rotation speed of 110r/min and fermentation time of 13 hours in an environment with a fermentation temperature of 67 ℃; the rapid fermentation residue had a moisture content of 10%. In the step 2, the nutrition supporting material is cotton seed hulls or broken corn cobs, and the volume ratio of the quick fermentation residues to the nutrition supporting material is as follows: 5: 1. the corncob fragments refer to corncobs crushed to 15 meshes. The volume ratio of the mixed material to the hypochlorous acid solution in the step 3 is as follows: 5: 1. the temperature in the high-pressure sterilization pot in the step 6 is 122.5 ℃, and the pressure is as follows: 1.05kgf/cm2The sterilization time was 2 hours.
Experimental example 1
Based on the embodiment 1 of the invention, the amount of wet garbage put in is 539.5kg, the amount of biological leaven put in is 53.9g, the operation time of the fermentation tank is as follows: 12 h; the resulting fast fermentation residue weighed 63.52kg, degradation rate: 88.23 percent.
Experimental example 2
Based on the example 3 of the invention, the amount of wet waste charged is 634kg, the amount of bio-starter added is 63.4g, the fermenter run time: 13 h; the weight of the obtained quick fermentation residue was 72.64kg, degradation rate: 88.54 percent.
Experimental example 3
Based on the embodiment 2 of the invention, the sterilized fungus bags are obtained in batches and inoculated, wherein the weight of each fungus bag is 450g (the dry weight of the material is 135g), and 25 bags are inoculated each time; the total experiment is 5 times, the average of the normal fruiting fungus bags is 24.5 bags, and the fruiting rate is as follows: 98 percent, the fruiting amount of each bag is 230g to 250g, and the fruiting amount is 1.7 to 1.85 times of that of the dry weight.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "connected," "connecting," and the like are to be construed broadly, and may, for example, be fixedly connected, integrally connected, or detachably connected; or communication between the interior of the two elements; they may be directly connected or indirectly connected through an intermediate, and those skilled in the art can understand the specific meaning of the above terms in the present invention according to specific situations. The above examples are merely illustrative of the feasible embodiments of the present invention and they are not intended to limit the scope of the present invention, and equivalent embodiments, modifications and alterations without departing from the technical spirit of the present invention are included in the scope of the present invention.
Claims (9)
1. A method for preparing an edible mushroom culture medium by utilizing wet garbage for rapid fermentation is characterized by comprising the following steps: the method comprises the following steps:
step 1: crushing, squeezing and dehydrating the wet garbage, then placing the wet garbage into a fermentation stirring tank, putting an inoculated biological fermentation agent into the fermentation stirring tank, and stirring and fermenting to obtain a quick fermentation residue;
step 2: mixing the rapid fermentation residue obtained in the step 1 with a nutritional support material to prepare a mixed material;
and step 3: spraying hypochlorous acid solution with the concentration of 1% on the mixed material for disinfection;
and 4, step 4: adding a quicklime suspension with the concentration of 1% into the mixed material sterilized in the step 3 to enable the pH value to reach 8-8.5;
and 5: bagging the material with the adjusted pH value;
step 6: placing the bagged fungus bags in an autoclave for sterilization;
and 7: ventilating and cooling the sterilized fungus bags in the step 6, and placing the cooled fungus bags on a sterile table to complete inoculation of oyster mushroom strains;
and 8: placing the inoculated fungus bags in a dark place with the ambient temperature of 25-27 ℃ and the ambient humidity of 80-85%, and observing the growth of edible fungus hyphae by naked eyes after 12 hours, wherein the hyphae grow over the fungus bags after 5-7 days;
and step 9: and (3) placing the fungus bag full of hypha in a ventilated place with the ambient temperature of 25-27 ℃ and the ambient humidity of 85-90% until the oyster mushroom grows.
2. The method for preparing edible fungus culture medium by utilizing wet garbage for rapid fermentation according to claim 1, wherein the method comprises the following steps: the moisture of the wet garbage after extrusion dehydration in the step 1 is 65-75%.
3. The method for preparing edible fungus culture medium by utilizing wet garbage for rapid fermentation according to claim 1, wherein the method comprises the following steps: the biological leavening agent in the step 1 comprises bacillus subtilis, bryophyte, bacillus vegicus, brevibacterium limosum, polymyxa and aspergillus, and the ratio of the bacillus subtilis, the bryophyte, the bacillus vegicus, the bacillus limosum, the polymyxa and the aspergillus is 3.5:2:1.5:1: 1.
4. The method for preparing edible fungus culture medium by utilizing wet garbage for rapid fermentation according to claim 3, wherein the method comprises the following steps: the adding amount of the biological leavening agent is 100 g/t.
5. The method for preparing edible fungus culture medium by utilizing wet garbage for rapid fermentation according to claim 1, wherein the method comprises the following steps: the stirring fermentation in the step 1 is to stir at a rotating speed of 100-120 r/min for 12-14 hours in an environment with a fermentation temperature of 60-75 ℃; the water content of the rapid fermentation residue is 8-12%.
6. The method for preparing edible fungus culture medium by utilizing wet garbage for rapid fermentation according to claim 1, wherein the method comprises the following steps: in the step 2, the nutrition supporting material is cotton seed hulls or broken corn cobs, and the volume ratio of the quick fermentation residues to the nutrition supporting material is as follows: 5: 1.
7. the method for preparing edible fungus culture medium by utilizing wet garbage for rapid fermentation according to claim 6, wherein the method comprises the following steps: the corncob fragments refer to corncobs crushed to 15 meshes.
8. The method for preparing edible fungus culture medium by utilizing wet garbage for rapid fermentation according to claim 1, wherein the method comprises the following steps: the volume ratio of the mixed material to the hypochlorous acid solution in the step 3 is as follows: 5: 1.
9. the method for preparing edible fungus culture medium by utilizing wet garbage for rapid fermentation according to claim 1, wherein the method comprises the following steps: the temperature in the high-pressure sterilization pot in the step 6 is 120-125 ℃, and the pressure is as follows: 0.9 to 1.1kgf/cm2The sterilization time was 2 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111331409.8A CN113875499A (en) | 2021-11-11 | 2021-11-11 | Method for preparing edible fungus culture medium by utilizing wet garbage to perform rapid fermentation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111331409.8A CN113875499A (en) | 2021-11-11 | 2021-11-11 | Method for preparing edible fungus culture medium by utilizing wet garbage to perform rapid fermentation |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113875499A true CN113875499A (en) | 2022-01-04 |
Family
ID=79017940
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111331409.8A Pending CN113875499A (en) | 2021-11-11 | 2021-11-11 | Method for preparing edible fungus culture medium by utilizing wet garbage to perform rapid fermentation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113875499A (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040035047A1 (en) * | 2002-08-20 | 2004-02-26 | Envitech, Inc. | Biotreatment of cheese-processing wastewater by cultivating mushroom mycelia |
| CN105130514A (en) * | 2015-07-21 | 2015-12-09 | 北京林业大学 | Secondary compost fertilizer and secondary compost method |
| CN106587366A (en) * | 2015-10-15 | 2017-04-26 | 百米马（天津）有限公司 | Special bacteria for leachate deodorization of garbage treatment plant and using method thereof |
| CN107502582A (en) * | 2017-10-11 | 2017-12-22 | 杨建设 | A kind of organic matter decomposing inoculant and preparation method thereof |
| CN109089732A (en) * | 2018-08-10 | 2018-12-28 | 华南理工大学 | A kind of culture medium of edible fungus and the preparation method and application thereof |
| CN110607250A (en) * | 2019-06-10 | 2019-12-24 | 江苏绿博生物科技有限公司 | Quadruple viable bacteria agent for treating kitchen waste and preparation method and application thereof |
-
2021
- 2021-11-11 CN CN202111331409.8A patent/CN113875499A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040035047A1 (en) * | 2002-08-20 | 2004-02-26 | Envitech, Inc. | Biotreatment of cheese-processing wastewater by cultivating mushroom mycelia |
| CN105130514A (en) * | 2015-07-21 | 2015-12-09 | 北京林业大学 | Secondary compost fertilizer and secondary compost method |
| CN106587366A (en) * | 2015-10-15 | 2017-04-26 | 百米马（天津）有限公司 | Special bacteria for leachate deodorization of garbage treatment plant and using method thereof |
| CN107502582A (en) * | 2017-10-11 | 2017-12-22 | 杨建设 | A kind of organic matter decomposing inoculant and preparation method thereof |
| CN109089732A (en) * | 2018-08-10 | 2018-12-28 | 华南理工大学 | A kind of culture medium of edible fungus and the preparation method and application thereof |
| CN110607250A (en) * | 2019-06-10 | 2019-12-24 | 江苏绿博生物科技有限公司 | Quadruple viable bacteria agent for treating kitchen waste and preparation method and application thereof |
Non-Patent Citations (1)
| Title |
|---|
| 钟振洋等: "《环卫机械设备合理选择与经济使用》", vol. 1, 中国建筑工业出版社, pages: 120 - 124 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104293694B (en) | A kind of preparation method of sludge aerobic compost composite bacteria agent | |
| CN105567612B (en) | A kind of degradation composite bacteria agent preparation of garden waste and application | |
| CN105002221B (en) | The method of intensive Vegetable area reclaiming organic waste efficient anaerobic fermentation production biogas | |
| CN105695369B (en) | A kind of complex micro organism fungicide for straw low temperature fermentation | |
| CN102409013B (en) | Organic material inoculant and preparation method thereof | |
| CN102942395A (en) | Preparation method of aerobic high-temperature static compost by using corrosion-promoting compost composite fermentation bacteria agent | |
| CN105567740A (en) | High-density solid fermentation method for agricultural bacillus | |
| CN104987157A (en) | Ganoderma lucidum antler culture medium utilizing domestic garbage and method for cultivating ganoderma lucidum antler | |
| CN103289937B (en) | Method for producing bacillus laterosporus live bacteria by high density solid fermentation | |
| CN104522299A (en) | Preparation method of wet-base biological active product | |
| CN101851121B (en) | Compound bacterial agent for efficiently converting pig excrements and preparation method and application thereof | |
| CN103583227A (en) | Method for cultivating good-quality high-yield hericium erinaceus by means of mulberry branches | |
| CN112028687A (en) | Kitchen waste prepared liquid organic fertilizer and production process thereof | |
| CN111296171A (en) | High-quality cultivation method for industrial grifola frondosa | |
| CN109234171B (en) | Preparation method of selenium-rich ganoderma lucidum mycelium powder | |
| CN111197007A (en) | Production method of high-efficiency bacterial bricks for composting | |
| CN102174460A (en) | Gliocladium roseum chlamydospore and method for producing wettable powder thereof | |
| CN114698752A (en) | Agricultural waste resource utilization method based on biological fermentation and insect transformation | |
| CN101828481B (en) | Method for preparing edible mushroom culture bar | |
| CN105886430A (en) | Method for producing bacillus amyloliquefaciens bacterial agent through solid-state fermentation | |
| CN106954736B (en) | Method for producing composite microecological active biological feed for ruminant by using waste mushroom residue | |
| CN108795812A (en) | A kind of compost decomposing agent and the preparation method and application thereof | |
| CN112194514A (en) | Bio-organic fertilizer starter and preparation method thereof | |
| CN111527991A (en) | Process for making edible reed fungus sticks | |
| CN113875499A (en) | Method for preparing edible fungus culture medium by utilizing wet garbage to perform rapid fermentation |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20220104 |
|
| RJ01 | Rejection of invention patent application after publication |