CN113860952A - Preparation method of hydrolyzed collagen polypeptide multi-effect foliar fertilizer based on graphene oxide activation - Google Patents
Preparation method of hydrolyzed collagen polypeptide multi-effect foliar fertilizer based on graphene oxide activation Download PDFInfo
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- CN113860952A CN113860952A CN202111290127.8A CN202111290127A CN113860952A CN 113860952 A CN113860952 A CN 113860952A CN 202111290127 A CN202111290127 A CN 202111290127A CN 113860952 A CN113860952 A CN 113860952A
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- graphene oxide
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 59
- 229920001184 polypeptide Polymers 0.000 title claims abstract description 58
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 58
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 58
- 102000008186 Collagen Human genes 0.000 title claims abstract description 53
- 108010035532 Collagen Proteins 0.000 title claims abstract description 53
- 229920001436 collagen Polymers 0.000 title claims abstract description 53
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 title claims abstract description 45
- 229910021389 graphene Inorganic materials 0.000 title claims abstract description 45
- 230000004913 activation Effects 0.000 title claims abstract description 28
- 238000002360 preparation method Methods 0.000 title claims abstract description 17
- 238000002156 mixing Methods 0.000 claims abstract description 29
- 239000007788 liquid Substances 0.000 claims abstract description 24
- 239000000243 solution Substances 0.000 claims abstract description 20
- 239000005648 plant growth regulator Substances 0.000 claims abstract description 18
- 229910052751 metal Inorganic materials 0.000 claims abstract description 17
- 239000002184 metal Substances 0.000 claims abstract description 17
- 239000012266 salt solution Substances 0.000 claims abstract description 17
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 16
- 238000003756 stirring Methods 0.000 claims abstract description 14
- 239000006185 dispersion Substances 0.000 claims abstract description 12
- 239000011259 mixed solution Substances 0.000 claims abstract description 11
- 238000001694 spray drying Methods 0.000 claims abstract description 7
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 12
- ZOIORXHNWRGPMV-UHFFFAOYSA-N acetic acid;zinc Chemical compound [Zn].CC(O)=O.CC(O)=O ZOIORXHNWRGPMV-UHFFFAOYSA-N 0.000 claims description 8
- RUTXIHLAWFEWGM-UHFFFAOYSA-H iron(3+) sulfate Chemical compound [Fe+3].[Fe+3].[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O.[O-]S([O-])(=O)=O RUTXIHLAWFEWGM-UHFFFAOYSA-H 0.000 claims description 8
- 229910000360 iron(III) sulfate Inorganic materials 0.000 claims description 8
- 239000004246 zinc acetate Substances 0.000 claims description 8
- CJUUXVFWKYRHAR-UHFFFAOYSA-M 1-Naphthaleneacetic acid sodium salt Chemical compound [Na+].C1=CC=C2C(CC(=O)[O-])=CC=CC2=C1 CJUUXVFWKYRHAR-UHFFFAOYSA-M 0.000 claims description 6
- 229920000858 Cyclodextrin Polymers 0.000 claims description 6
- 229920001202 Inulin Polymers 0.000 claims description 6
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims description 6
- 239000004115 Sodium Silicate Substances 0.000 claims description 6
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 6
- DFPAKSUCGFBDDF-ZQBYOMGUSA-N [14c]-nicotinamide Chemical compound N[14C](=O)C1=CC=CN=C1 DFPAKSUCGFBDDF-ZQBYOMGUSA-N 0.000 claims description 6
- 239000011609 ammonium molybdate Substances 0.000 claims description 6
- 235000018660 ammonium molybdate Nutrition 0.000 claims description 6
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 claims description 6
- 229940010552 ammonium molybdate Drugs 0.000 claims description 6
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 6
- 239000004327 boric acid Substances 0.000 claims description 6
- 239000004202 carbamide Substances 0.000 claims description 6
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 claims description 6
- 229940029339 inulin Drugs 0.000 claims description 6
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 6
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 6
- 229940099596 manganese sulfate Drugs 0.000 claims description 6
- 239000011702 manganese sulphate Substances 0.000 claims description 6
- 235000007079 manganese sulphate Nutrition 0.000 claims description 6
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- 239000011591 potassium Substances 0.000 claims description 6
- 229910052700 potassium Inorganic materials 0.000 claims description 6
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 6
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 6
- 239000002994 raw material Substances 0.000 claims description 6
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 claims description 6
- NTHWMYGWWRZVTN-UHFFFAOYSA-N sodium silicate Chemical compound [Na+].[Na+].[O-][Si]([O-])=O NTHWMYGWWRZVTN-UHFFFAOYSA-N 0.000 claims description 6
- 229910052911 sodium silicate Inorganic materials 0.000 claims description 6
- WCUXLLCKKVVCTQ-UHFFFAOYSA-M Potassium chloride Chemical compound [Cl-].[K+] WCUXLLCKKVVCTQ-UHFFFAOYSA-M 0.000 claims description 4
- 239000000463 material Substances 0.000 claims description 4
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 4
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 claims description 4
- ONDPHDOFVYQSGI-UHFFFAOYSA-N zinc nitrate Chemical compound [Zn+2].[O-][N+]([O-])=O.[O-][N+]([O-])=O ONDPHDOFVYQSGI-UHFFFAOYSA-N 0.000 claims description 4
- IXVMHGVQKLDRKH-VRESXRICSA-N Brassinolide Natural products O=C1OC[C@@H]2[C@@H]3[C@@](C)([C@H]([C@@H]([C@@H](O)[C@H](O)[C@H](C(C)C)C)C)CC3)CC[C@@H]2[C@]2(C)[C@@H]1C[C@H](O)[C@H](O)C2 IXVMHGVQKLDRKH-VRESXRICSA-N 0.000 claims description 2
- OORIKNJWZHTXDC-UHFFFAOYSA-N CCCC(CC)C(CC)(C(=O)OCC)N Chemical compound CCCC(CC)C(CC)(C(=O)OCC)N OORIKNJWZHTXDC-UHFFFAOYSA-N 0.000 claims description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 2
- 229910021578 Iron(III) chloride Inorganic materials 0.000 claims description 2
- IXVMHGVQKLDRKH-KNBKMWSGSA-N brassinolide Chemical compound C1OC(=O)[C@H]2C[C@H](O)[C@H](O)C[C@]2(C)[C@H]2CC[C@]3(C)[C@@H]([C@H](C)[C@@H](O)[C@H](O)[C@@H](C)C(C)C)CC[C@H]3[C@@H]21 IXVMHGVQKLDRKH-KNBKMWSGSA-N 0.000 claims description 2
- 229910000365 copper sulfate Inorganic materials 0.000 claims description 2
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 2
- 238000004108 freeze drying Methods 0.000 claims description 2
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims description 2
- 239000001103 potassium chloride Substances 0.000 claims description 2
- 235000011164 potassium chloride Nutrition 0.000 claims description 2
- 239000004323 potassium nitrate Substances 0.000 claims description 2
- 235000010333 potassium nitrate Nutrition 0.000 claims description 2
- 239000011592 zinc chloride Substances 0.000 claims description 2
- 235000005074 zinc chloride Nutrition 0.000 claims description 2
- -1 compound sodium nitrophenolate Chemical class 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 5
- 229910021645 metal ion Inorganic materials 0.000 abstract description 4
- 238000006243 chemical reaction Methods 0.000 abstract description 2
- 235000015097 nutrients Nutrition 0.000 description 10
- 230000004720 fertilization Effects 0.000 description 7
- 238000000034 method Methods 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 230000012010 growth Effects 0.000 description 5
- 239000002689 soil Substances 0.000 description 5
- 238000010521 absorption reaction Methods 0.000 description 4
- 230000000052 comparative effect Effects 0.000 description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 4
- 230000009920 chelation Effects 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 208000024891 symptom Diseases 0.000 description 3
- 230000002411 adverse Effects 0.000 description 2
- 230000001965 increasing effect Effects 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 239000002077 nanosphere Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 239000000575 pesticide Substances 0.000 description 2
- 235000006008 Brassica napus var napus Nutrition 0.000 description 1
- 240000000385 Brassica napus var. napus Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 231100000674 Phytotoxicity Toxicity 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000009418 agronomic effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 244000038559 crop plants Species 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 125000000524 functional group Chemical group 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000018343 nutrient deficiency Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- AXKBOWBNOCUNJL-UHFFFAOYSA-M sodium;2-nitrophenolate Chemical compound [Na+].[O-]C1=CC=CC=C1[N+]([O-])=O AXKBOWBNOCUNJL-UHFFFAOYSA-M 0.000 description 1
- 239000002881 soil fertilizer Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
Abstract
The invention discloses a preparation method of a hydrolyzed collagen polypeptide multi-effect foliar fertilizer based on graphene oxide activation, which comprises the following steps: adding hydrolyzed collagen polypeptide and a metal salt solution into a graphene oxide dispersion solution, stirring and mixing for 5-10 min, then adding into a microwave ultrasonic mixing reactor, and reacting at 40-45 ℃ for 30-45 min to obtain a mixed solution; and step two, mixing the mixed solution, the stress resistance agent and the plant growth regulator, stirring, and then carrying out spray drying to obtain the hydrolyzed collagen polypeptide multi-effect foliar fertilizer based on graphene oxide activation. Adding hydrolyzed collagen polypeptide, a metal salt solution and graphene oxide dispersion liquid into a microwave ultrasonic mixing reactor for reaction, so that metal ions are chelated on the hydrolyzed collagen polypeptide and are simultaneously combined with graphene oxide; and further mixing the fertilizer with a stress-resistant agent and a plant growth regulator to obtain the multi-effect foliar fertilizer chelated based on the hydrolyzed collagen polypeptide, wherein the multi-effect foliar fertilizer has an obvious yield-increasing effect on the rapes.
Description
Technical Field
The invention relates to the technical field of leaf fertilizers, and particularly relates to a preparation method of a hydrolyzed collagen polypeptide multi-effect leaf fertilizer based on graphene oxide activation.
Background
In general, plants absorb nutrients in soil or nutrient solution mainly through root systems, and supply crops for growth and development. Besides the root system, the leaves, stems and leaves of the plant can absorb various nutrient components, and the absorption effect is better than that of the root system. The fertilizer or nutrient components required by plants are prepared into nutrient solution with certain concentration according to a proportion by taking the foliar absorption of crops as a way, and the fertilizer for foliar fertilization is called foliar fertilizer. The foliar fertilizer belongs to a fertilizer for external roots. Compared with the traditional rhizosphere fertilizer, the foliar fertilizer has unique advantages, and firstly, the foliar fertilizer with strong pertinence can determine the types and the formulas of nutrients according to the abundance condition of soil nutrients, the soil fertilizer supply capacity, the absorption strength of crops on the soil nutrients and the fertilizer and nutrient elements lacking in the crops, so that the nutrients lacking in the crops can be supplemented in time, and the nutrient deficiency symptom of the crops can be relieved or eliminated. Secondly, because the foliar fertilizer is directly applied on the surface of the plant, the speed of the foliar fertilizer is about 1 time faster than the speed of absorbing fertilizer by the root system. And the fertilizer effect is more easily applied to other parts. And thirdly, the risk of environmental pollution is less than that of the traditional root system fertilization, and the soil is not easy to cause acid alkalization, salinization and the like. Fourthly, the application method of the foliar fertilizer is simple and convenient, the economic application method is not influenced by the growth period of crops, and the agricultural production investment is reduced. The foliar fertilization can be carried out in most growth periods of crops, especially the root fertilization is inconvenient after the crop plants grow up and form ridges, and the foliar fertilization is basically not influenced by the height, density and the like of the plants.
The multi-effect foliar fertilizer is an agricultural composition, in particular to a fertilizer and a pesticide in the composition. A multi-effect foliar fertilizer is a multi-effect nutritional anti-adversity composition applied to the leaf surfaces of crops, scientifically combines a plurality of high-quality inorganic fertilizers, plant growth regulators and the like according to the agronomic requirements of preventing and solving adverse symptoms and enhancing resistance in the growth process of the crops, supplements the nutrition of the crops, solves the problem of crop deficiency caused by unreasonable fertilization, relieves the fertilizer damage and phytotoxicity caused by fertilization and improper pesticide application, simultaneously enhances the anti-adversity and disease and insect resistance capabilities of the crops such as cold resistance, drought resistance, moisture resistance and the like, prevents the occurrence of adverse symptoms such as mosaic, yellow leaf, lobule, stiff seedling, stump setting, flower shortage and the like, and realizes the high yield of the crops. However, the absorption effect of the existing multi-effect foliar fertilizer is not good, and the growth and the yield of crops are influenced.
Disclosure of Invention
An object of the present invention is to solve at least the above problems and/or disadvantages and to provide at least the advantages described hereinafter.
To achieve these objects and other advantages in accordance with the present invention, there is provided a method for preparing a hydrolyzed collagen polypeptide multi-effect foliar fertilizer based on graphene oxide activation, comprising the steps of:
step one, adding hydrolyzed collagen polypeptide into graphene oxide dispersion liquid, stirring and mixing for 30-45 min to obtain premixed feed liquid;
adding the premixed material liquid into a microwave ultrasonic mixing reactor, simultaneously adding a metal salt solution, and then reacting for 30-45 min at the temperature of 40-45 ℃ to obtain a mixed liquid;
and step three, mixing the mixed solution, the stress resistance agent and the plant growth regulator, stirring, and then performing spray drying or freeze drying to obtain the hydrolyzed collagen polypeptide multi-effect foliar fertilizer based on graphene oxide activation.
Preferably, the concentration of the graphene oxide dispersion liquid is 3-5 mg/mL; the mass-to-volume ratio of the hydrolyzed collagen polypeptide to the graphene oxide dispersion liquid is 6-8 mg:1 mL; the concentration of the metal salt solution is 3-4 mg/mL; the mass-volume ratio of the hydrolyzed collagen polypeptide to the metal salt solution is 6-8 mg:1 mL.
Preferably, the microwave power in the microwave ultrasonic mixing reactor is 300-500W, the ultrasonic power is 300-600W, the ultrasonic frequency adopts dual-frequency alternating frequency which is 35-45 kHz and 135-145 kHz respectively, and the time for the dual-frequency alternating treatment is the frequency switched every 5 min.
Preferably, the metal salt solution is at least one of a potassium chloride solution, a potassium nitrate solution, a calcium chloride solution, a ferric sulfate solution, a ferric chloride solution, a zinc acetate solution, a copper sulfate solution, a zinc nitrate solution and a zinc chloride solution.
Preferably, in the third step, 60-120 parts by weight of the mixed solution, 15-25 parts by weight of the stress resistance agent and 3-6 parts by weight of the plant growth regulator are mixed, stirred and then spray-dried or freeze-dried to obtain the hydrolyzed collagen polypeptide multi-effect foliar fertilizer based on graphene oxide activation.
Preferably, the stress resistance agent is characterized by comprising the following raw materials in parts by weight: 8-12 parts of monopotassium phosphate, 6-8 parts of manganese sulfate, 1-3 parts of magnesium sulfate, 2-4 parts of boric acid, 1-2 parts of cyclodextrin, 2-4 parts of ammonium molybdate, 1-3 parts of nicotinamide, 1-3 parts of potassium fulvate, 1-2 parts of polypeptide urea, 1-2 parts of sodium silicate and 2-4 parts of inulin;
preferably, the plant growth regulator is at least one of sodium naphthalene acetate, sodium nitrophenolate, brassinolide and diethyl aminoethyl hexanoate.
The invention at least comprises the following beneficial effects: according to the invention, hydrolyzed collagen polypeptide, a metal salt solution and a graphene oxide dispersion liquid are added into a microwave ultrasonic mixing reactor for reaction, so that metal ions are chelated on the hydrolyzed collagen polypeptide, and graphene oxide is simultaneously combined, the graphene oxide is added in the microwave ultrasonic mixing reactor, so that rich functional groups and a higher specific surface area of the graphene oxide are utilized to provide active sites or spaces for the load of effective ingredients of subsequent fertilizers, the load capacity and stability of the fertilizers are further increased, the loss of the effective ingredients is avoided, the soil is polluted, the utilization rate of the fertilizers is improved, and meanwhile, the graphene oxide is further mixed with a stress-resistant agent and a plant growth regulator to obtain a multi-effect foliar fertilizer based on the hydrolyzed collagen polypeptide chelation, and the multi-effect foliar fertilizer has a remarkable yield-increasing effect on oilseed rape
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention.
Description of the drawings:
FIG. 1 is a flow chart of a preparation method of a hydrolyzed collagen polypeptide multi-effect foliar fertilizer based on graphene oxide activation;
the specific implementation mode is as follows:
the present invention is further described in detail below with reference to examples so that those skilled in the art can practice the invention with reference to the description.
It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.
The preparation method of the hydrolyzed collagen polypeptide comprises the following steps: a method for preparing collagen polypeptide nanospheres by hydrolyzing collagen polypeptide in a method for preparing collagen polypeptide nanospheres by using CN201410836224.6 leather-making waste skin collagen.
Example 1:
a preparation method of a hydrolyzed collagen polypeptide multi-effect foliar fertilizer based on graphene oxide activation comprises the following steps:
step one, adding 6000mg of hydrolyzed collagen polypeptide into 1000mL of graphene oxide dispersion liquid with the concentration of 4mg/mL, and stirring and mixing for 30min to obtain premixed feed liquid;
adding the premixed material liquid into a microwave ultrasonic mixing reactor, simultaneously adding 1000mL of metal salt solution (3mg/mL), and reacting at the temperature of 45 ℃ for 45min to obtain a mixed liquid; the microwave power and the ultrasonic power in the microwave ultrasonic wave mixing reactor are respectively 300W and 300W, the ultrasonic frequency adopts double-frequency alternating frequency which is respectively 35kHz and 135kHz, and the time of the double-frequency alternating treatment is that the frequency is switched every 5 min; the metal salt solution is a mixed solution of ferric sulfate and zinc acetate, wherein the mass ratio of the ferric sulfate to the zinc acetate is 1: 1; according to the invention, the chelation of the hydrolyzed collagen polypeptide and metal ions is realized by utilizing the cavitation of ultrasonic waves and the high-speed oscillation principle of microwaves, so that the metal ions can be chelated on the hydrolyzed collagen polypeptide more completely;
step three, mixing 1.2kg of mixed liquor, 0.2kg of stress resistance agent and 0.05kg of plant growth regulator, stirring, and then carrying out spray drying to obtain the hydrolyzed collagen polypeptide multi-effect foliar fertilizer based on graphene oxide activation;
the stress resistance agent comprises the following raw materials: 8kg of monopotassium phosphate, 6kg of manganese sulfate, 1kg of magnesium sulfate, 2kg of boric acid, 1kg of cyclodextrin, 2kg of ammonium molybdate, 1kg of nicotinamide, 1kg of potassium fulvate, 1kg of polypeptide urea, 1kg of sodium silicate and 2kg of inulin;
the plant growth regulator is sodium naphthalene acetate.
Example 2:
a preparation method of a hydrolyzed collagen polypeptide multi-effect foliar fertilizer based on graphene oxide activation comprises the following steps:
step one, adding 8000mg of hydrolyzed collagen polypeptide into 1000mL of graphene oxide dispersion liquid with the concentration of 4mg/mL, and stirring and mixing for 30min to obtain premixed feed liquid;
adding the premixed material liquid into a microwave ultrasonic mixing reactor, simultaneously adding 1000mL of metal salt solution (4mg/mL), and reacting at the temperature of 45 ℃ for 30min to obtain a mixed liquid; the microwave power and the ultrasonic power in the microwave ultrasonic wave mixing reactor are respectively 300W and 300W, the ultrasonic frequency adopts double-frequency alternating frequency which is respectively 35kHz and 135kHz, and the time of the double-frequency alternating treatment is that the frequency is switched every 5 min; the metal salt solution is a mixed solution of ferric sulfate and zinc acetate, wherein the mass ratio of the ferric sulfate to the zinc acetate is 1: 1;
step three, mixing 1kg of mixed liquor, 0.25kg of stress resistance agent and 0.06kg of plant growth regulator, stirring, and then spray drying to obtain the hydrolyzed collagen polypeptide multi-effect foliar fertilizer based on graphene oxide activation;
the stress resistance agent comprises the following raw materials: 10kg of monopotassium phosphate, 7kg of manganese sulfate, 2kg of magnesium sulfate, 3kg of boric acid, 1kg of cyclodextrin, 3kg of ammonium molybdate, 2kg of nicotinamide, 1kg of potassium fulvate, 1kg of polypeptide urea, 1kg of sodium silicate and 3kg of inulin;
the plant growth regulator is sodium naphthalene acetate.
Comparative example 1:
a preparation method of a hydrolyzed collagen polypeptide multi-effect foliar fertilizer based on graphene oxide activation comprises the following steps:
step one, adding 8000mg of hydrolyzed collagen polypeptide into 2000mL of water, stirring and mixing for 30min, then adding into a microwave ultrasonic mixing reactor, and reacting for 30min at the temperature of 45 ℃ to obtain a mixed solution; the microwave power and the ultrasonic power in the microwave ultrasonic wave mixing reactor are respectively 300W and 300W, the ultrasonic frequency adopts double-frequency alternating frequency which is respectively 35kHz and 135kHz, and the time of the double-frequency alternating treatment is that the frequency is switched every 5 min;
step two, mixing 1kg of mixed liquor, 0.25kg of stress resistance agent and 0.06kg of plant growth regulator, stirring, and then carrying out spray drying to obtain the hydrolyzed collagen polypeptide multi-effect foliar fertilizer based on graphene oxide activation;
the stress resistance agent comprises the following raw materials: 10kg of monopotassium phosphate, 7kg of manganese sulfate, 2kg of magnesium sulfate, 3kg of boric acid, 1kg of cyclodextrin, 3kg of ammonium molybdate, 2kg of nicotinamide, 1kg of potassium fulvate, 1kg of polypeptide urea, 1kg of sodium silicate and 3kg of inulin;
the plant growth regulator is sodium naphthalene acetate.
Comparative example 2:
a preparation method of a hydrolyzed collagen polypeptide multi-effect foliar fertilizer based on graphene oxide activation comprises the following steps:
step one, adding 8000mg of hydrolyzed collagen polypeptide into 1000mL of graphene oxide dispersion liquid with the concentration of 4mg/mL, and stirring and mixing for 30 min; obtaining a premixed feed liquid;
step two, adding 1000mL of metal salt solution (4mg/mL) into the premix solution, and reacting for 30min at the temperature of 45 ℃ to obtain a mixed solution; the metal salt solution is a mixed solution of ferric sulfate and zinc acetate, wherein the mass ratio of the ferric sulfate to the zinc acetate is 1: 1;
step two, mixing 1kg of mixed liquor, 0.25kg of stress resistance agent and 0.06kg of plant growth regulator, stirring, and then carrying out spray drying to obtain the hydrolyzed collagen polypeptide multi-effect foliar fertilizer based on graphene oxide activation;
the stress resistance agent comprises the following raw materials: 10kg of monopotassium phosphate, 7kg of manganese sulfate, 2kg of magnesium sulfate, 3kg of boric acid, 1kg of cyclodextrin, 3kg of ammonium molybdate, 2kg of nicotinamide, 1kg of potassium fulvate, 1kg of polypeptide urea, 1kg of sodium silicate and 3kg of inulin;
the plant growth regulator is sodium naphthalene acetate.
Effective component tests are carried out on the hydrolyzed collagen polypeptide multi-effect foliar fertilizer based on graphene oxide activation obtained in the example 2 and the comparative examples 1-2, and the results are shown in table 1;
TABLE 1
In a rape test field of southwest science and technology university, the applicant adopts the hydrolyzed collagen polypeptide multi-effect foliar fertilizer based on graphene oxide activation of the embodiment 2 and the comparative examples 1-2 of the invention to carry out a test (when in use, 40-45 kg of water is added to every 100 g of the multi-effect foliar fertilizer), and in a paddy field with a root and a root, the test is carried out according to the test requirements specified by the ministry of agriculture, and clear water spraying is used as a contrast, and the specific data is shown in table 2;
TABLE 2
Compared with clear water, the multiple-effect foliar fertilizer based on the hydrolyzed collagen polypeptide chelation, which is prepared by the invention, is applied to rape fields, and the yield increasing effect on rape is obvious.
While embodiments of the invention have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable to various fields of endeavor for which the invention may be embodied with additional modifications as would be readily apparent to those skilled in the art, and the invention is therefore not limited to the details given herein and to the examples shown and described without departing from the generic concept as defined by the claims and their equivalents.
Claims (7)
1. A preparation method of a hydrolyzed collagen polypeptide multi-effect foliar fertilizer based on graphene oxide activation is characterized by comprising the following steps:
step one, adding hydrolyzed collagen polypeptide into graphene oxide dispersion liquid, stirring and mixing for 30-45 min to obtain premixed feed liquid;
adding the premixed material liquid into a microwave ultrasonic mixing reactor, simultaneously adding a metal salt solution, and then reacting for 30-45 min at the temperature of 40-45 ℃ to obtain a mixed liquid;
and step three, mixing the mixed solution, the stress resistance agent and the plant growth regulator, stirring, and then performing spray drying or freeze drying to obtain the hydrolyzed collagen polypeptide multi-effect foliar fertilizer based on graphene oxide activation.
2. The preparation method of the hydrolyzed collagen polypeptide multi-effect foliar fertilizer based on graphene oxide activation as claimed in claim 1, wherein the concentration of the graphene oxide dispersion liquid is 3-5 mg/mL; the mass-to-volume ratio of the hydrolyzed collagen polypeptide to the graphene oxide dispersion liquid is 6-8 mg:1 mL; the concentration of the metal salt solution is 3-4 mg/mL; the mass-volume ratio of the hydrolyzed collagen polypeptide to the metal salt solution is 6-8 mg:1 mL.
3. The preparation method of the hydrolyzed collagen polypeptide multi-effect foliar fertilizer based on graphene oxide activation as claimed in claim 1, wherein the microwave power in the microwave ultrasonic mixing reactor is 300-500W, the ultrasonic power is 300-600W, the ultrasonic frequency adopts dual-frequency alternating frequencies which are 35-45 kHz and 135-145 kHz respectively, and the time for the dual-frequency alternating treatment is to switch the frequency once every 5 min.
4. The preparation method of the hydrolyzed collagen polypeptide multi-effect foliar fertilizer based on graphene oxide activation as claimed in claim 1, wherein the metal salt solution is at least one of potassium chloride solution, potassium nitrate solution, calcium chloride solution, ferric sulfate solution, ferric chloride solution, zinc acetate solution, copper sulfate solution, zinc nitrate solution and zinc chloride solution.
5. The preparation method of the hydrolyzed collagen polypeptide multi-effect foliar fertilizer based on graphene oxide activation as claimed in claim 1, wherein in the third step, 60-120 parts by weight of the mixed solution, 15-25 parts by weight of the stress-resistant agent and 3-6 parts by weight of the plant growth regulator are mixed, stirred, and then spray-dried or freeze-dried to obtain the hydrolyzed collagen polypeptide multi-effect foliar fertilizer based on graphene oxide activation.
6. The preparation method of the hydrolyzed collagen polypeptide multi-effect foliar fertilizer based on graphene oxide activation as claimed in claim 1, wherein the stress tolerance comprises the following raw materials in parts by weight: 8-12 parts of monopotassium phosphate, 6-8 parts of manganese sulfate, 1-3 parts of magnesium sulfate, 2-4 parts of boric acid, 1-2 parts of cyclodextrin, 2-4 parts of ammonium molybdate, 1-3 parts of nicotinamide, 1-3 parts of potassium fulvate, 1-2 parts of polypeptide urea, 1-2 parts of sodium silicate and 2-4 parts of inulin.
7. The preparation method of the hydrolyzed collagen polypeptide multi-effect foliar fertilizer based on graphene oxide activation as claimed in claim 1, wherein the plant growth regulator is at least one of sodium naphthalene acetate, compound sodium nitrophenolate, brassinolide and diethyl aminoethyl hexanoate.
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| CN113248304A (en) * | 2021-05-14 | 2021-08-13 | 台沃科技集团股份有限公司 | Multi-effect nutritional composition for preventing and treating actinidia chinensis root cancer and application thereof |
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| CN106747954A (en) * | 2016-11-25 | 2017-05-31 | 广西田园生化股份有限公司 | A kind of foliar fertilizer of graphene-containing nano material |
| CN112142515A (en) * | 2020-09-27 | 2020-12-29 | 陕西科技大学 | Polypeptide chelated trace element foliar fertilizer as well as preparation method and use method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106747954A (en) * | 2016-11-25 | 2017-05-31 | 广西田园生化股份有限公司 | A kind of foliar fertilizer of graphene-containing nano material |
| CN112142515A (en) * | 2020-09-27 | 2020-12-29 | 陕西科技大学 | Polypeptide chelated trace element foliar fertilizer as well as preparation method and use method thereof |
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| CN113248304A (en) * | 2021-05-14 | 2021-08-13 | 台沃科技集团股份有限公司 | Multi-effect nutritional composition for preventing and treating actinidia chinensis root cancer and application thereof |
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