CN113854397A

CN113854397A - Method for extracting corn protein concentrate by gluten water

Info

Publication number: CN113854397A
Application number: CN202111178140.4A
Authority: CN
Inventors: 赵春德; 常书强; 吴芳; 穆国伟; 康玉民; 程佳
Original assignee: SHANDONG SCENTS JIANYUAN BIO-TECH CO LTD
Current assignee: SHANDONG SCENTS JIANYUAN BIO-TECH CO LTD
Priority date: 2021-10-09
Filing date: 2021-10-09
Publication date: 2021-12-31

Abstract

A method for extracting corn protein concentrate by gluten water comprises the following steps: the method comprises the following steps: adjusting pH of the gluten water to 4-8, adding alpha amylase, and reacting at 70-100 deg.C for 30-150min to obtain enzymatic hydrolysate; step two: adding alkali liquor into the enzymolysis liquid obtained in the step one to adjust the pH value to 8-12, and reacting at the temperature of 40-70 ℃ for 30-60min to obtain a corn concentrated protein liquid; step three: and centrifugally separating the concentrated corn protein liquid obtained in the step two, washing with water, and drying to obtain the concentrated corn protein. The invention uses alpha-amylase to enzymolyze starch in corn gluten water, and reduces the dry fat content from 2.5% to about 0.5-1% after degreasing by an alkaline method. After water washing and centrifugal treatment, the color of the corn protein powder is obviously reduced, the corn protein powder can reach faint yellow to creamy yellow after spray drying, and the dry basis protein content can reach more than 90 percent after enzymolysis and starch removal and alkali degreasing. The sulfide content of the corn concentrated protein powder product can be reduced to below the national standard limit.

Description

Method for extracting corn protein concentrate by gluten water

Technical Field

The invention belongs to the field of recycling of gluten water in corn starch production, and particularly relates to a method for extracting corn concentrated protein from gluten water.

Background

In 2018, the consumption of Chinese protein products reaches 8829.6 ten thousand tons, and the consumption of protein products in European Union and America respectively reaches 4426.7 ten thousand tons and 4134.9 ten thousand tons. The consumption of protein products will further increase in the future, and the supply gap of Chinese protein products in 2030 is expected to reach more than 3800 ten thousand tons. The vegetable protein has the health advantages of zero cholesterol, low fat and the like, reduces the risk of diseases caused by meat products, and meets the concept of green and healthy consumption. The edible corn protein powder is produced by taking the corn gluten meal as a raw material, so that the corn resource is comprehensively utilized, the problem of lack of protein resource is solved, and the demand of people on healthy plant protein is met. Corn is one of main crops which have been used for producing starch, a large amount of gluten water is generated when the corn is used for producing the starch by wet grinding at present, the dry-based starch content in the gluten water is about 15 percent, the dry-based protein content is about 65 percent and the dry-based fat content is about 2.5 percent according to the detection of the formula, so the gluten water has great recycling value, and a gluten water recycling process is lacked at present.

Disclosure of Invention

The invention provides a method for extracting corn protein concentrate by gluten water, which is used for overcoming the defects in the prior art.

The invention is realized by the following technical scheme:

a method for extracting corn protein concentrate by gluten water comprises the following steps:

the method comprises the following steps: adjusting pH of the gluten water to 4-8, adding alpha amylase, and reacting at 70-100 deg.C for 30-150min to obtain enzymatic hydrolysate;

step two: adding alkali liquor into the enzymolysis liquid obtained in the step one to adjust the pH value to 8-12, and reacting at the temperature of 40-70 ℃ for 30-60min to obtain a corn concentrated protein liquid;

step three: and centrifugally separating the concentrated corn protein liquid obtained in the step two, washing with water, and drying to obtain the concentrated corn protein.

The method for extracting the corn protein concentrate by the gluten water comprises the step one, wherein the addition amount of the alpha amylase is 100-1000U/kg.

The method for extracting the corn protein concentrate from the gluten water comprises the step three, wherein the washing times are 1-5 times, and the ratio of the washing materials to the washing liquids is 1: 1-6.

The invention has the advantages that: the invention uses the gluten water of the starch produced by corn wet milling as the raw material to produce the corn concentrated protein. The invention uses alpha-amylase to carry out enzymolysis on starch in corn gluten water, carries out enzymolysis on starch components into soluble sugar such as dextrin and the like, and removes the components through water washing so as to improve the dry basis protein content in the corn protein powder. After the starch is enzymolyzed, the dry starch content of the corn protein powder can be reduced to about 0.5-1.5%, and the dry protein content can be increased to about 88-91%. After degreasing by an alkaline method, the content of dry fat is reduced from 2.5 percent to about 0.5 to 1 percent. After water washing and centrifugal treatment, the color of the corn protein powder is obviously reduced, the corn protein powder can reach faint yellow to creamy yellow after spray drying, and the dry basis protein content can reach more than 90 percent after enzymolysis and starch removal and alkali degreasing. The sulfide content of the corn concentrated protein powder product can be reduced to below the national standard limit.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly introduced below, and it is obvious that the drawings in the following description are some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

FIG. 1 is one of the zein test reports of the present invention;

FIG. 2 is a second assay report for zein in accordance with the present invention;

FIG. 3 is a third report of the zein test of the present invention;

fig. 4 is one of the gluten water test reports of the present invention;

fig. 5 is a second gluten water test report according to the present invention;

fig. 6 is a gluten water test report three of the present invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

The method comprises the following steps: adjusting the pH value of the gluten water to 4-8, adding alpha amylase according to the addition amount of 100-1000U/kg, and reacting at the temperature of 70-100 ℃ for 30-150min to obtain an enzymatic hydrolysate;

step three: and (4) centrifugally separating the concentrated corn protein liquid obtained in the step two, washing the concentrated corn protein liquid for 1-5 times according to the washing feed liquid ratio of 1:1-6, and drying the solid to obtain the concentrated corn protein. Example 2

After a large amount of enzymolysis conditions were investigated, the optimum enzymolysis conditions were obtained, as shown in table one.

Watch 1

After a large number of enzymatic hydrolysis conditions were investigated, the optimum water washing conditions were obtained as shown in tables two and three.

Watch two

	Sample weight/g	Optically active 1	Optically active 2	Optically active 3	Optically active 4	Average	Content of wet basis%	Content on a dry basis%
									Centrifugal precipitation	3.6562	0.201	0.198	0.195	0.174	0.192	1.4224％	5.0251％
Washing once	4.1111	0.126	0.138	0.152	0.113	0.13225	0.8713％	3.4350％
									Washing twice	4.0756	0.108	0.106	0.105	0.1	0.10475	0.6961％	2.8291％
Washed with water for three times	3.9656	0.105	0.094	0.090	0.108	0.09925	0.6779％	2.8275％
									Four times of water washing	4.9987	0.038	0.038	0.042	0.046	0.041	0.2222％	0.9228％

Watch III

Changes of starch, protein and fat after alkaline degreasing are shown in the fourth table, the fifth table and the sixth table.

Watch four

	Sample weight/g	Titration amount/ml	Wet basis content/%	Dry basis content/%)
					Protein content	0.338	7.27	20.0624％	91.4854％

Watch five

	Bottle weight/g	Sample weight/g	Bottle + sample weight/g	Crude fat content/%)
					Fat content	72.4961	1.3649	72.5053	0.6740％

Watch six

As can be seen from the above verification and fig. 1 to 6, the present invention prepares a concentrated protein with a higher content and a higher purity with gluten water having a high protein content, thereby achieving the full utilization of resources.

Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.

Claims

1. A method for extracting corn protein concentrate by gluten water is characterized in that: the method comprises the following steps:

2. The method for extracting corn protein concentrate from gluten water as claimed in claim 1, wherein: the addition amount of the alpha amylase in the step one is 100-1000U/kg.

3. The method for extracting corn protein concentrate from gluten water as claimed in claim 1, wherein: the washing times in the third step are 1-5 times, and the ratio of washing materials to liquid is 1: 1-6.