CN101390564A - Production method of corn separation protein - Google Patents
Production method of corn separation protein Download PDFInfo
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- CN101390564A CN101390564A CNA2007101512562A CN200710151256A CN101390564A CN 101390564 A CN101390564 A CN 101390564A CN A2007101512562 A CNA2007101512562 A CN A2007101512562A CN 200710151256 A CN200710151256 A CN 200710151256A CN 101390564 A CN101390564 A CN 101390564A
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Abstract
The invention takes the by-product corn gluten (also known as corn gluten meal, containing about 60% protein) produced in the corn starch production as raw material and adopts the alkali-heat treatment, so that the insoluble amylose and amylopectin in the corn gluten can be gelatinized and removed in the high-temperature aqueous alkali, and the oil and fat suspension in the corn gluten is processed with saponification reaction in the high-temperature aqueous alkali to make oil and fat separation; moreover, the structure of the fat-soluble yellow pigment in the corn gluten is damaged under high-temperature and high-alkaline conditions, so the fat-soluble yellow pigment is water-soluble and removed; after repeated washing, purifying, separating and drying processes, the corn-isolated protein with the protein content reaching more than 90% is obtained; therefore, the invention develops a new protein material for the food industry.
Description
Technical field
The present invention relates to a kind of corn separated protein powder new product and extraction process technology thereof, belong to deep-processing technical field of agricultural products.
Background technology
At present, the corn annual production of China is about 1.1 hundred million tons, accounts for 20% of Gross World Product, occupies second, and wherein nearly more than 400 ten thousand tons are used for producing starch.The accessory substance of cornstarch is called corn protein powder, and (Corn gluten meal CGM), claims again " maize yellow-powder ".Corn protein powder contains the protein about 60%, deficiency such as, color and luster many because of its impurity and mouthfeel be all poor, lack essential amino acids such as lysine, tryptophan, biological value is low, had a strong impact on it in Application in Food Industry, corn protein powder is mainly used in feed industry at present.
Corn protein powder is that corn makes coarse starch milk through wet grinding technology, obtains seitan water through Separation of Proteins again, then concentrate drying and making.Main accessory substance during as corn wet method producing starch, its protein content is greatly about about 60%.Except that containing abundant protide nutriment, still contain other inorganic salts and multivitamin in the corn protein powder.Main jade contains alcohol soluble protein (zein, 68%), glutelin (glutelin, 28%) and globulin (globulins, 1.2%) and albumin four kinds of albumen such as (albumin, trace) in the corn protein powder.Utilize the main protein composition alcohol soluble protein and the water-fast character of glutelin of corn protein powder, reach the purpose of purification of protein, thereby the rough zein of low value is changed into high-purity, edible corn separated protein powder, improved the application and the economic worth of zein.For achieving the above object, former researcher carries out many researchs." research of New Processing Technology of Corn-Gluten Foaming Powder " (grain and feed industry that Li Yan carries out, 2002.3) in, with corn gluten meal, be that maize yellow-powder is a raw material,, remove the starch in the maize yellow-powder with α-amylase liquefaction hydrolysis, use the basic hydrolysis zein again, separate by milipore filter, technologies such as spray-drying are made partial hydrolysis, flaxen zein foam powder, and purity reaches 93.6%.This process using enzyme hydrolysis starch, and then starch removed, the cost of enzyme must be calculated.Compare with centrifugal separation equipment, use the milipore filter device, will improve equipment investment and cost greatly.Protein is through basic hydrolysis, and part generates peptide and free amino acid, compares with former albumen, and its physico-chemical property changes or partly.Li Yuanzhi etc. with lab scale simulation cornstarch market technology, obtain corn gluten (slurry before the maize yellow-powder drying) in the research of " the high efficiente callback method of corn edible protein and new purposes in the yellow slurry " (China Agricultural University's journal, 1992.7).Make protein coagulating by pH or the adding protein coagulating agent glucolactone of adjusting corn gluten then, separation, drying obtain edible corn protein powder at last again, and sample only contains the protein about 45%, belongs to rough protein articles.You Xin is in the technology of integrate extraction method for oil and albumen powder " a kind of from maize germ " patent (CN1907055A), with maize germ is raw material, go out corn oil with the alcohol lixiviate, dissolve protein with alkali, adjust pH with acid solution again, be settled out protein, by isolated by filtration, low temperature drying obtains corn protein powder.Used raw material maize germ in the patent is the raw material that is different from the conventional corn bloom, and does not point out the protein content of resulting corn protein powder in the patent.
Summary of the invention
Because the protein content of maize yellow-powder is lower, generally is about 60%, and color jaundice and have foreign odor flavor beastly, limited its application in food industry.Therefore, the present invention carries out operations such as degreasing, destarch to bloom bring up to more than 90% purity of protein, and color and smell are effectively handled, and prepares the corn protein isolate.
The major impurity of maize yellow-powder is starch (15～25%), fat and uranidin (7～15%), and increasing percent protein is exactly to remove starch, fat and uranidin.Purification theoretical foundation of the present invention is: starch divides straight chain and amylopectin, and amylose dissolves in hot water, and amylopectin also dissolves in hot water under pressurized conditions.Utilize this character starch-containing matter protein raw materials of purifying, make the stripping of starch high temperature, and alkali condition can promote starch gelatinization.The maize yellow-powder suspension adds alkali under hot conditions, saponification takes place, and grease is separated.Uranidin in the maize yellow-powder belongs to fat-soluble pigment, under the high-alkali condition of high temperature destructurized generation water-soluble, thereby reach the purpose of separation.
The invention provides a kind of production method of corn protein isolate, this production method cost is low, efficient height, product yield height, protein content height, quality better.
Of the present invention is a kind of production method of corn protein isolate, it is characterized in that, it comprises following operating procedure and process conditions:
The first step with water and maize yellow-powder to mixed, to rise: the solid-liquid ratio of kilogram counts 100: 6～12, and adjusting the pH scope is more than 8, is heated to 50～100 ℃ and be incubated stirring in water bath 20～60 minutes;
Second step is with the high speed centrifugation while hot of the feed liquid after the stirring in water bath, through centrifugation operation back centrifugate layering, the superiors are grease uranidin, the intermediate layer is starch dissolution fluid and the water-soluble pigment molecular of destroyed generation, the bottom mainly is not water-soluble alcohol soluble protein and glutelin, abandon supernatant, get the orlop insoluble slag;
The 3rd step is because a water-bath centrifugation is insufficient, getting orlop insoluble slag after for the first time centrifugal again adds water and stirs, to rise: the solid-liquid ratio of kilogram counts 100: 6～and 12, be heated to 50～100 ℃ and be incubated stirring in water bath 20～60 minutes, the situation of centrifugation is led to step 2, abandon supernatant, get the orlop insoluble slag;
The 4th step was dried to powder with insoluble slag, can get the corn separated protein powder by freeze drying, pneumatic conveying drying or spray-drying.
Be applicable to that raw material of the present invention comprises corn, corn flour, dry corn protein powder (the title maize yellow-powder is arranged), semiarid corn protein powder or the undried corn protein powder of crossing.The kind of raw material, quality and protein content will produce certain influence to the quality and the yield of finished product.
The present invention compared with prior art has following advantage and beneficial effect:
1, raw material is the accessory substance corn protein powder that cornstarch factory produces, and can be dry the mistake, also can be that partial desiccation is crossed, and raw material sources are extensive, are not subjected to the restriction of production area;
2, the present invention has broken through in the past that enzyme process removes two step purification routes of starch, saponification grease removal, and single stepping realizes that albumen purifies, and has avoided the brown stain that produces because of Maillard reaction, has reduced the amylase raw material, has shortened production process, greatly reduces cost;
3, adopt centrifugal separation equipment to separate, with respect to milipore filter appliance arrangement small investment.
4, the present invention finally realizes five general objectives, that is: purifying protein removes fat, destarch, and decolouring eliminate the unusual smell, and the albumen yield is about 70-80%.
The present invention has that production cost is low, efficient is high, the yield advantages of higher.Produce raw materials used byproduct, improved the added value of maize yellow-powder widely for corn processing.Simultaneously, the present invention is purified to zein content more than 90%, and the protein content of product reaches the standard of protein isolate, so be called the corn protein isolate, have been widely used in field of food industry, the production of having filled up high-purity corn protein isolate is blank with application.
The specific embodiment
Below in conjunction with example, the present invention is described in further details.
The inventor has carried out deep creative research and test to the present invention, and many successful embodiment are arranged, and enumerates five specific embodiments below.
Embodiment 1
Get corn protein powder 150g, add entry 2.5L, stir.Regulate material liquid pH to 8 with NaOH solution, feed liquid is heated to 50 ℃, and insulation stirring in water bath 60min.Feed liquid through the centrifugal 20min of centrifuge, is abandoned supernatant, collect the orlop insoluble slag, add water 2.5Kg with continuing in the insoluble slag again, stir, 50 ℃ of insulation stirring in water bath 60min.Feed liquid through the centrifugal 20min of centrifuge, is abandoned supernatant, collect the orlop insoluble slag.Spray-drying gets 98g corn separated protein powder.
Embodiment 2
Get the corn protein powder of 150g, add 1.5L water, use the homogenizer homogeneous.Regulate material liquid pH to 11 with NaOH solution, feed liquid is heated to 80 ℃, and insulation stirring in water bath 40min.Feed liquid through the centrifugal 30min of supercentrifuge, is abandoned supernatant, collect the insoluble slag of orlop.1.5Kg water will be continued to add again in the insoluble slag, 80 ℃ of insulation stirring in water bath 40min; Feed liquid through the centrifugal 30min of centrifuge, is abandoned supernatant, collect the orlop insoluble slag; Vacuum drying is pulverized, and gets 105g corn separated protein powder.
Embodiment 3
Get the corn protein powder of 600g, add 5L water, use mulser emulsification.Regulate material liquid pH to 13 with NaOH, feed liquid is heated to 100 ℃, and insulation stirring in water bath 20min.Feed liquid is centrifugal through supercentrifuge 15min, go supernatant, collect the insoluble slag of orlop.1.25Kg water will be continued to add again in the insoluble slag, 100 ℃ of insulation stirring in water bath 20min.Feed liquid through the centrifugal 15min of centrifuge, is abandoned supernatant, collect the orlop insoluble slag.Be dried to powder, get 415g corn separated protein powder.
Claims (6)
1, a kind of corn protein isolate technology of extracting from maize yellow-powder (CGM, corn gluten meal) is characterized in that comprising the following steps:
(1) water is mixed with maize yellow-powder, make its solid content more than 6%; Adjust acidity to more than the pH8.Feed liquid is heated more than 50 ℃, and insulation stirring in water bath 20～60min
(2) feed liquid is centrifugal on supercentrifuge, abandon supernatant, collect water-fast, mainly contain the solid material of alcohol soluble protein and Gu Bai.Water will expect to transfer to solid minute content admittedly more than 6%; Feed liquid is heated more than 50 ℃, and more than the insulation stirring in water bath 20min; Feed liquid is centrifugal on supercentrifuge, collect solid material.
(3) insoluble solid material is dried to powder, obtains the corn separated protein powder at last.
2, according to the described technology that must from maize yellow-powder, prepare the corn protein isolate of claim 1, it is characterized in that maize yellow-powder water furnishing solid content in the feed liquid more than 6%; Major impurity starch, fat and the uranidin of stripping maize yellow-powder separate the major impurity of maize yellow-powder and protein under alkalescence (more than the pH8), heating (more than 50 ℃, and being incubated more than the stirring in water bath 20min) condition.
3, according to claim 1, the 2 described technologies that prepare the corn protein isolate from maize yellow-powder, it is characterized in that using supercentrifuge centrifugal the feed liquid of stripping impurity, abandoning supernatant is collected solid material.
4, according to claim 1,2,3 described from maize yellow-powder the technologies of preparation corn protein isolate, it is characterized in that the proteinaceous solid material separating through repeatedly washing, further remove impurity, improve lipidated protein.
5,, it is characterized in that by adopting the corn protein drying after spray-drying or methods such as pneumatic conveying drying or freeze drying will be purified to make pulverous corn protein isolate according to claim 1,2,3, the 4 described technologies that from maize yellow-powder, prepare the corn protein isolate.
6, according to claim 1,2,3,4,5 described from maize yellow-powder the technologies of preparation corn protein isolate, it is characterized in that the specification of quality of the corn separated protein powder prepared is: protein (in butt) 〉=90%; Moisture≤6.0%; Starch≤4.0%; Fat≤3.0%.
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| CN 200710151256 CN101390564B (en) | 2007-09-18 | 2007-09-18 | Production method of corn separation protein |
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| CN101390564B CN101390564B (en) | 2011-01-26 |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101703146B (en) * | 2009-10-30 | 2011-11-02 | 华南理工大学 | Method for purifying food-grade corn protein powder |
| CN102793055A (en) * | 2011-12-22 | 2012-11-28 | 江南大学 | Method for degreasing and decoloring distillers dried grains with soluble (DDGS) |
| CN104039164A (en) * | 2011-11-14 | 2014-09-10 | 龟田制菓株式会社 | Rice-protein composition and method for manufacturing same |
| CN104938763A (en) * | 2014-03-28 | 2015-09-30 | 中粮营养健康研究院有限公司 | Method for separating proteins from corn original pulp |
| CN105053570A (en) * | 2015-08-28 | 2015-11-18 | 安徽丰原马鞍山生物化学有限公司 | Corn gluten meal nutritional solution |
| CN105961844A (en) * | 2016-05-04 | 2016-09-28 | 沈喆如 | Feed with hydrolyzed protein and preparation method of feed |
| CN109749964A (en) * | 2019-02-26 | 2019-05-14 | 润盈生物工程（上海）有限公司 | Improve the medium treatment method of lactobacillus acidophilus fermentation and freeze drying viable microorganism number |
| CN113854397A (en) * | 2021-10-09 | 2021-12-31 | 山东香驰健源生物科技有限公司 | Method for extracting corn protein concentrate by gluten water |
| US11453707B2 (en) | 2016-01-28 | 2022-09-27 | Cofco Nutrition And Health Research Institute Co., Ltd. | Protein product and preparation method thereof |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1268762C (en) * | 2003-12-19 | 2006-08-09 | 吉林大学 | Corn peptide and its preparation and use |
| CN1292673C (en) * | 2004-07-14 | 2007-01-03 | 哈尔滨中冠生物工程有限公司 | Process for preparing active peptide of corn protein |
| CN100369928C (en) * | 2005-12-30 | 2008-02-20 | 深圳职业技术学院 | Blood pressure lowering polypeptide of corn gluten powder, its separation method and application |
-
2007
- 2007-09-18 CN CN 200710151256 patent/CN101390564B/en active Active
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101703146B (en) * | 2009-10-30 | 2011-11-02 | 华南理工大学 | Method for purifying food-grade corn protein powder |
| CN104039164A (en) * | 2011-11-14 | 2014-09-10 | 龟田制菓株式会社 | Rice-protein composition and method for manufacturing same |
| CN102793055A (en) * | 2011-12-22 | 2012-11-28 | 江南大学 | Method for degreasing and decoloring distillers dried grains with soluble (DDGS) |
| CN104938763A (en) * | 2014-03-28 | 2015-09-30 | 中粮营养健康研究院有限公司 | Method for separating proteins from corn original pulp |
| CN104938763B (en) * | 2014-03-28 | 2018-08-21 | 中粮营养健康研究院有限公司 | The method of protein isolate from corn primary-pulp |
| CN105053570A (en) * | 2015-08-28 | 2015-11-18 | 安徽丰原马鞍山生物化学有限公司 | Corn gluten meal nutritional solution |
| US11453707B2 (en) | 2016-01-28 | 2022-09-27 | Cofco Nutrition And Health Research Institute Co., Ltd. | Protein product and preparation method thereof |
| CN105961844A (en) * | 2016-05-04 | 2016-09-28 | 沈喆如 | Feed with hydrolyzed protein and preparation method of feed |
| CN109749964A (en) * | 2019-02-26 | 2019-05-14 | 润盈生物工程（上海）有限公司 | Improve the medium treatment method of lactobacillus acidophilus fermentation and freeze drying viable microorganism number |
| CN113854397A (en) * | 2021-10-09 | 2021-12-31 | 山东香驰健源生物科技有限公司 | Method for extracting corn protein concentrate by gluten water |
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| CN101390564B (en) | 2011-01-26 |
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Address after: 100015 Beijing, Jiuxianqiao Middle Road, building 24, No., building 6 Patentee after: China National Academy of Food & Fermentation Industries Address before: 100027 Beijing city Chaoyang District Xiaoyun Road No. 32 Patentee before: China National Academy of Food & Fermentation Industries |
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Address after: No. 32, Xiaoyun Road, Chaoyang District, Beijing 100015 Patentee after: China Food Fermentation Industry Research Institute Co., Ltd. Address before: 100015 Beijing, Jiuxianqiao Middle Road, building 24, No., building 6 Patentee before: China National Academy of Food & Fermentation Industries |