CN113846136B - Kasugamycin fermentation medium and fermentation method - Google Patents
Kasugamycin fermentation medium and fermentation method Download PDFInfo
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- CN113846136B CN113846136B CN202111353659.1A CN202111353659A CN113846136B CN 113846136 B CN113846136 B CN 113846136B CN 202111353659 A CN202111353659 A CN 202111353659A CN 113846136 B CN113846136 B CN 113846136B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/46—Preparation of O-glycosides, e.g. glucosides having an oxygen atom of the saccharide radical bound to a cyclohexyl radical, e.g. kasugamycin
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
The invention relates to a kasugamycin fermentation medium and a fermentation method, which belong to the field of antibiotic fermentation production, wherein the medium comprises the following two parts by volume percent: 2.5 to 6.0 percent of soybean cake powder, 0.05 to 0.1 percent of monopotassium phosphate, 0.1 to 0.5 percent of sodium chloride, 0.5 to 1.0 percent of corn oil, 0.15 to 0.25 percent of sodium citrate, 0.5 to 1.0 percent of corn steep liquor dry powder, 0.015 to 0.025 percent of GPE and pH: naturally, the formula of the feed supplement comprises: the formula of the feed supplement comprises the following components: glucose powder 0.5-1.0%, sodium citrate 0.15-0.25%, ammonia water (concentration 25% for adjusting pH) 0.15-0.25%. According to the invention, the sodium citrate is added into the culture medium, so that the kasugamycin titer is improved by more than 15%, the sugar consumption is reduced by more than 25%, the fermentation period is shortened by 36-48 hours, the production cost is greatly reduced, and the production efficiency is improved.
Description
Technical Field
The invention belongs to the field of antibiotic fermentation production, and particularly relates to a kasugamycin fermentation medium and a fermentation method.
Background
The kasugamycin is produced by streptomyces spring, belongs to aminoglycoside antibiotics, is widely applied to agriculture, is particularly remarkable in preventing and treating rice blast, and is also widely applied to apples, potatoes, citrus and the like. It is harmless to human and livestock, has no residue and no pollution, is one of the green pesticides with application prospect at present, and is popular with users at home and abroad.
The kasugamycin is produced by adopting a liquid fermentation method, and the existing fermentation process has the following problems:
1) The industrialized fermentation level is low, and the fermentation titer is about 12000 ug/mL;
2) The fermentation period is relatively long, typically around 250 hours;
3) The production energy consumption is high, the cost is high, the control requirement is high, and the market competitiveness is lacking.
The Chinese patent application CN201810817178 discloses a method for improving the biological titer of kasugamycin, wherein a common medium of streptomyces parviflora consists of soybean meal, naCl, corn steep liquor dry powder, maltose, fish oil, KHPO and inositol, and the optimized medium is formed by adding growth factors and microelements on the basis of the common medium. Streptomyces parvulus is cultivated in a medium added with growth factors and microelements, the kasugamycin titer is improved by 19.7%, and the single pot yield is improved by 10.8%. The culture medium can be used for culturing streptomyces parviflorus, and the biological potency and single-pot yield of kasugamycin can be obviously improved.
Chinese patent CN201410707264 discloses a kasugamycin fermentation medium, wherein the fermentation medium contains tween 80 and a defoaming agent, wherein the defoaming agent is an organosilicon defoaming agent and/or a polyether defoaming agent. The kasugamycin fermentation medium provided by the invention obviously reduces the foam production in the liquid fermentation process through the synergistic effect of Tween 80, the defoamer and the culture medium raw materials, thereby improving the oxygen transfer efficiency, better avoiding the liquid escape and bacteria contamination caused by a large amount of foam, and obviously improving the fermentation yield of the kasugamycin by utilizing the fermentation medium and the culture method provided by the invention.
Chinese patent application CN201711346595 relates to a kasugamycin fermentation medium and a fermentation method, which comprises the following steps: (1) preparing a culture medium, and sterilizing the culture medium; the formula of the culture medium is as follows: 5.0 to 8.0 percent of soybean meal powder; naCl:0.3 to 0.5 percent; corn steep liquor dry powder: 0.5 to 1.0 percent; maltose: 2.0 to 2.5 percent; fish oil: 3.5 to 4.0 percent; KHPO:0.03 to 0.05 percent; inositol: 0.01% -0.05%; the pH value is 6.8-7.2; (2) Inoculating the streptomyces parvulus strain in a culture medium, fermenting for 168-170 h at 28-30 ℃ with the inoculum size of 10-15 vol%, filtering the fermentation product, and taking filtrate to obtain the final product. The invention realizes the improvement of the fermentation titer of kasugamycin and shortens the fermentation period, thereby reducing the production cost and improving the product quality.
These studies have all attempted from different angles to solve the problems associated with the existing kasugamycin fermentation process, but are still not comprehensive, and there is still a need in the art for more efficient and optimized solutions.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides a kasugamycin fermentation medium and a fermentation method, which effectively improve the titer of the kasugamycin, reduce the sugar consumption, shorten the fermentation period, greatly reduce the production cost and improve the production efficiency.
In order to achieve the aim of the invention, the invention adopts the following technical scheme: a kasugamycin fermentation medium consists of the following components in percentage by volume; 2.5 to 6.0 percent of soybean cake powder, 0.05 to 0.1 percent of monopotassium phosphate, 0.1 to 0.5 percent of sodium chloride, 0.5 to 1.0 percent of glucose powder, 0.15 to 0.25 percent of ammonia water, 0.5 to 1.0 percent of corn oil, 0.15 to 0.25 percent of sodium citrate, 0.5 to 1.0 percent of corn steep liquor dry powder, 0.015 to 0.025 percent of polyoxypropylene polyoxyethylene glyceryl ether GPE and pH:6.8-7.3.
In a preferred embodiment of the present invention, the mass concentration of ammonia is 25% for adjusting the pH.
In the preferred embodiment of the invention, in the culture medium, 3.0% of soybean meal, 0.075% of monopotassium phosphate, 0.3% of sodium chloride, 0.75% of corn oil, 0.8% of corn steep liquor dry powder, 0.02% of polyoxypropylene polyoxyethylene glyceryl ether GPE, 0.2% of ammonia water and 0.2% of sodium citrate.
In a preferred embodiment of the present invention, the method for preparing the kasugamycin fermentation medium comprises:
(1) accurately weighing soybean cake powder, potassium dihydrogen phosphate, sodium chloride, corn oil, corn steep liquor dry powder and sodium citrate, adding GPE, and fixing volume with tap water;
(2) preparing glucose solution, split charging ammonia water, and fixing volume by tap water;
(3) the pH is natural before digestion.
The invention also provides a fermentation method of the kasugamycin fermentation medium, which comprises the following steps of:
(1) Preparing a fermentation basic culture medium, wherein the formula comprises the following components in percentage by volume: 2.5 to 6.0 percent of soybean cake powder, 0.05 to 0.1 percent of monopotassium phosphate, 0.1 to 0.5 percent of sodium chloride, 0.5 to 1.0 percent of corn oil, 0.5 to 1.0 percent of corn steep liquor dry powder, 0.015 to 0.025 percent of polyoxypropylene polyoxyethylene glyceryl ether GPE, and tap water for volume fixation, pH: naturally; preparing a feed supplement culture medium, wherein glucose powder is prepared into a glucose solution, and the mass percentage of the glucose powder is 0.5-1.0%; then adding sodium citrate into the glucose solution, wherein the mass percent of the sodium citrate is 0.15-0.25%, and then adding 0.15-0.25% ammonia water to adjust the pH value;
(2) And (3) sterilization: the fermentation basic culture medium is maintained for 30min at the temperature of 121-130 ℃; the feed supplement culture medium is maintained at 110 ℃ for 30min;
(3) Inoculating, cooling to 30 ℃ after sterilization, and inoculating streptomyces parviflorus with the inoculum size of 5-15vol%;
(4) Fermentation conditions: temperature: 28-30 ℃, the aeration ratio is 1:0.5-1:1.5, the stirring rotation speed is 150-350rpm, the tank pressure is 0.03-0.05MPa, and the fermentation period is 160-170h; after fermentation, collecting fermentation products to obtain fermentation filtrate;
(5) Fermentation feeding conditions: fermenting and culturing for 20-30 hours, and continuously adding glucose and sodium citrate mixed solution and ammonia water when the pH is reduced to 6.8-7.3, and maintaining the pH between 6.8-7.3.
In a preferred embodiment of the present invention, in the step (1), the mass concentration of ammonia is 25% for adjusting the pH.
In a preferred embodiment of the invention, the kasugamycin inoculum size is 15%.
In a preferred embodiment of the present invention, sodium citrate is added to the kasugamycin feed medium in an amount of 0.20-0.25%.
Compared with the prior art, the invention has the following beneficial effects:
on the one hand, the fermentation medium of the invention consists of two major parts, namely a fermentation basic formula (soybean cake powder 2.5-6.0%, monopotassium phosphate 0.05-0.1%, sodium chloride 0.1-0.5%, corn oil 0.5-1.0%, sodium citrate 0.15-0.25%, corn steep liquor dry powder 0.5-1.0%, polyoxypropylene polyoxyethylene glyceryl ether GPE0.015-0.025%, pH: natural) and a feed supplement formula (glucose powder 0.5-1.0%, sodium citrate 0.15-0.25%, ammonia water 0.15-0.25%). According to the invention, the sodium citrate is added into the kasugamycin feed medium, so that the fermentation titer of the kasugamycin is effectively improved.
On the other hand, the invention optimizes the fermentation method and fermentation parameters aiming at specific fermentation medium composition, adds auxiliary materials once, reduces practical operation and trouble, adopts the culture medium and the fermentation method to produce the kasugamycin, improves the kasugamycin titer by more than 20%, reduces the sugar consumption by more than 25%, shortens the fermentation period by 36-48 hours, and greatly saves the production cost.
Detailed Description
The present invention will be described in further detail with reference to examples, but the present invention is not limited to the following examples.
Example 1
(1) Preparing a culture medium, preparing 35L of basic culture medium according to the formula proportion of the kasugamycin liquid fermentation culture medium, and putting the basic culture medium into a 50L fermentation tank for later use. The formula of the culture medium comprises: basic culture medium, bean cake powder 2.5%, potassium dihydrogen phosphate 0.05%, sodium chloride 0.1%, corn oil 0.5%, corn slurry dry powder 0.5%, polyoxypropylene polyoxyethylene glyceryl ether GPE 0.015%, pH natural, feed supplement culture medium, weighing glucose powder 0.5%, sodium citrate 0.15%, mixing with tap water to constant volume 5L for standby, and ammonia water (concentration 25%) 1L for standby.
(2) And (3) sterilization: the basic culture medium is sterilized at 121 ℃ for 30min, the feed medium is a glucose and sodium citrate mixed culture medium at 110 ℃ for 30min, and ammonia water is not sterilized.
(3) Inoculating, cooling the temperature of the fermentation tank to 30 ℃, inoculating streptomyces parvulus seed liquid, and fermenting and culturing with the inoculum size of 15 vol%.
(4) Fermentation culture: temperature 28 ℃, ventilation ratio 1:1, stirring at 150rpm, pressing at 0.05MPa, fermenting and culturing for 24 hours, starting to flow glucose and sodium citrate mixed solution when the pH is 7.0, naturally flowing ammonia water, controlling the pH to be 6.8-7.3, fermenting for 160 hours, collecting fermentation products, filtering to obtain filtrate, measuring 13800u/mL by adopting a liquid chromatography, and measuring the sugar consumption to be 0.5% and 205mL by adopting ammonia water.
Under the above operating conditions, when glucose and sodium citrate are not added when the pH is 7.0, ammonia water is naturally fed and added to control the pH to be 6.8-7.3, the fermentation period is 160 hours, the fermentation product is collected and filtered to obtain filtrate, and 12900u/mL is measured by adopting a liquid chromatography and is far lower than the titer of the application.
Example 2
(1) Preparing a culture medium, preparing 35L of the culture medium according to the formula proportion of the kasugamycin liquid fermentation culture medium, and putting the culture medium into a 50L fermentation tank for standby. The formula of the culture medium comprises: basic culture medium, bean cake powder 3.5%, potassium dihydrogen phosphate 0.05%, sodium chloride 0.1%, corn oil 0.5%, corn slurry dry powder 0.5%, polyoxypropylene polyoxyethylene glyceryl ether GPE 0.025%, pH natural, feed supplement culture medium, glucose powder 0.8%, sodium citrate 0.20%, mixing with tap water to constant volume 5L for standby, and ammonia water (concentration 25%) 1L for standby.
(2) And (3) sterilization: the basic culture medium is sterilized at 121 ℃ for 30min, the feed medium is a glucose and sodium citrate mixed culture medium at 110 ℃ for 30min, and ammonia water is not sterilized.
(3) Inoculating, cooling the temperature of the fermentation tank to 30 ℃, inoculating streptomyces parvulus seed liquid, and fermenting and culturing with the inoculum size of 15 vol%.
(4) Fermentation culture: temperature 28 ℃, ventilation ratio 1:1, stirring at 150rpm, pressing at 0.05MPa, fermenting and culturing for 24 hours, starting to flow glucose and sodium citrate mixed solution when the pH is 7.1, naturally flowing ammonia water, controlling the pH to be 6.8-7.3, fermenting for 160 hours, collecting fermentation products, filtering to obtain filtrate, measuring 13904u/mL by adopting a liquid chromatography, and measuring the sugar consumption to be 0.8% and the ammonia water to be 217mL.
Under the above operating conditions, no glucose and sodium citrate group is added when the pH is 7.1, the pH is controlled to be 6.8-7.3 by natural running-in of ammonia water, the fermentation period is 160 hours, the fermentation product is collected and filtered to obtain filtrate, and the titer of 12560u/mL is measured by liquid chromatography and is far lower than that of the application.
Example 3
(1) Preparing a culture medium, preparing 35L of the culture medium according to the formula proportion of the kasugamycin liquid fermentation culture medium, and putting the culture medium into a 50L fermentation tank for standby. The formula of the culture medium comprises: basic culture medium, bean cake powder 4.5%, potassium dihydrogen phosphate 0.05%, sodium chloride 0.1%, corn oil 0.5%, corn slurry dry powder 0.8%, polyoxypropylene polyoxyethylene glyceryl ether GPE 0.025%, pH natural, feed supplement culture medium, weighing glucose powder 1.0%, sodium citrate 0.25%, mixing with tap water to constant volume 5L for standby, and ammonia water (concentration 25%) 1L for standby.
(2) And (3) sterilization: the basic culture medium is sterilized at 121 ℃ for 30min, the feed medium is a glucose and sodium citrate mixed culture medium at 110 ℃ for 30min, and ammonia water is not sterilized.
(3) Inoculating, cooling the temperature of the fermentation tank to 30 ℃, inoculating streptomyces parvulus seed liquid, and fermenting and culturing with the inoculum size of 15 vol%.
(4) Fermentation culture: temperature 28 ℃, ventilation ratio 1:1, stirring at 150rpm, pressing at 0.05MPa, fermenting and culturing for 24 hours, starting to flow glucose and sodium citrate mixed solution when the pH is 7.2, naturally flowing ammonia water, controlling the pH to be 6.8-7.3, fermenting for 160 hours, collecting fermentation products, filtering to obtain filtrate, measuring 13900u/mL by adopting a liquid chromatography, and measuring the sugar consumption to be 1.0% and 225mL by adopting ammonia water.
Example 4
(1) Preparing a culture medium, preparing 35L of the culture medium according to the formula proportion of the kasugamycin liquid fermentation culture medium, and putting the culture medium into a 50L fermentation tank for standby. The formula of the culture medium comprises: basic culture medium, bean cake powder 5.5%, potassium dihydrogen phosphate 0.05%, sodium chloride 0.1%, corn oil 1.0%, corn slurry dry powder 1.0%, polyoxypropylene polyoxyethylene glyceryl ether GPE 0.025%, pH natural, feed supplement culture medium, weighing glucose powder 1.0%, sodium citrate 0.25%, mixing with tap water to constant volume 5L for standby, and ammonia water (concentration 25%) 1L for standby.
(2) And (3) sterilization: the basic culture medium is sterilized at 121 ℃ for 30min, the feed medium is a glucose and sodium citrate mixed culture medium at 110 ℃ for 30min, and ammonia water is not sterilized.
(3) Inoculating, cooling the temperature of the fermentation tank to 30 ℃, inoculating streptomyces parvulus seed liquid, and fermenting and culturing with the inoculum size of 15 vol%.
(4) Fermentation culture: temperature 28 ℃, ventilation ratio 1:1, stirring at 150rpm, pressing at 0.03MPa, fermenting and culturing for 24 hours, starting to flow glucose and sodium citrate mixed solution when the pH is 6.9, naturally flowing ammonia water, controlling the pH to be 6.8-7.3, fermenting for 160 hours, collecting fermentation products, filtering to obtain filtrate, measuring 14007u/mL by adopting a liquid chromatography, and measuring the sugar consumption to be 1.0% and 253mL by adopting ammonia water.
Example 5
(1) Preparing a culture medium, preparing 35L of the culture medium according to the formula proportion of the kasugamycin liquid fermentation culture medium, and putting the culture medium into a 50L fermentation tank for standby. The formula of the culture medium comprises: basic culture medium, bean cake powder 5.5%, potassium dihydrogen phosphate 0.1%, sodium chloride 0.1%, corn oil 1.0%, corn slurry dry powder 1.0%, polyoxypropylene polyoxyethylene glyceryl ether GPE 0.025%, pH natural, feed supplement culture medium, weighing glucose powder 1.0%, sodium citrate 0.25%, mixing with tap water to constant volume 5L for standby, and ammonia water (concentration 25%) 1L for standby.
(2) And (3) sterilization: the basic culture medium is sterilized at 121 ℃ for 30min, the feed medium is a glucose and sodium citrate mixed culture medium at 110 ℃ for 30min, and ammonia water is not sterilized.
(3) Inoculating, cooling the temperature of the fermentation tank to 30 ℃, inoculating streptomyces parvulus seed liquid, and fermenting and culturing with the inoculum size of 15 vol%.
(4) Fermentation culture: temperature 28 ℃, ventilation ratio 1:1, stirring at 150rpm, pressing at 0.05MPa, fermenting and culturing for 24 hours, starting to flow glucose and sodium citrate mixed solution when the pH is 6.8, naturally flowing ammonia water, controlling the pH to be 6.8-7.3, fermenting for 160 hours, collecting fermentation products, filtering to obtain filtrate, measuring 14507u/mL by adopting a liquid chromatography, and measuring the sugar consumption to be 1.0% and the ammonia water to be 230mL.
Example 6
(1) Preparing a culture medium, preparing 35L of the culture medium according to the formula proportion of the kasugamycin liquid fermentation culture medium, and putting the culture medium into a 50L fermentation tank for standby. The formula of the culture medium comprises: basic culture medium, bean cake powder 5.5%, potassium dihydrogen phosphate 0.1%, sodium chloride 0.1%, corn oil 1.0%, corn slurry dry powder 1.0%, polyoxypropylene polyoxyethylene glyceryl ether GPE 0.025%, pH natural, feed supplement culture medium, weighing glucose powder 1.0%, sodium citrate 0.25%, mixing with tap water to constant volume 5L for standby, and ammonia water (concentration 25%) 1L for standby.
(2) And (3) sterilization: the basic culture medium is sterilized at 121 ℃ for 30min, the feed medium is a glucose and sodium citrate mixed culture medium at 110 ℃ for 30min, and ammonia water is not sterilized.
(3) Inoculating, cooling the temperature of the fermentation tank to 30 ℃, inoculating streptomyces parvulus seed liquid, and fermenting and culturing with the inoculum size of 15 vol%.
(4) Fermentation culture: temperature 28 ℃, ventilation ratio 1:0.8, stirring at 150rpm, tank pressure of 0.05MPa, fermenting and culturing for 24 hours, wherein the pH value is reduced to 6.8, the mixed solution of glucose and sodium citrate is fed, the pH value is controlled to be 6.8-7.3 by natural feeding of ammonia water, the fermentation period is 160 hours, fermentation products are collected, filtrate is obtained by filtering, 14808u/mL is measured by adopting a liquid chromatography, the sugar consumption is 1.0%, and the ammonia water is 245mL.
While the invention has been described in detail in terms of general description, specific embodiments and experiments, it will be apparent to those skilled in the art that modifications and improvements can be made thereto. Accordingly, it is intended to claim all such modifications and improvements as may be made without departing from the scope of the invention.
Claims (6)
1. The kasugamycin fermentation medium is characterized by comprising a fermentation basic medium and a feed medium, wherein the fermentation basic medium comprises the following formula: 2.5 to 6.0 percent of soybean cake powder, 0.05 to 0.1 percent of monopotassium phosphate, 0.1 to 0.5 percent of sodium chloride, 0.5 to 1.0 percent of corn oil, 0.5 to 1.0 percent of corn steep liquor dry powder, 0.015 to 0.025 percent of polyoxypropylene polyoxyethylene glyceryl ether GPE, tap water for volume fixation, pH: naturally; the formula of the feed medium is as follows: glucose powder 0.5-1.0%, sodium citrate 0.15-0.25%, and ammonia water 0.15-0.25% are added to adjust pH value to 6.8-7.3.
2. The kasugamycin fermentation medium according to claim 1, wherein in the medium, soybean cake powder 3.0%, potassium dihydrogen phosphate 0.075%, sodium chloride 0.3%, corn oil 0.75%, corn steep liquor dry powder 0.8%, polyoxypropylene polyoxyethylene glyceryl ether GPE 0.02%, ammonia water 0.2%, sodium citrate 0.2%.
3. A fermentation method of a kasugamycin fermentation medium, comprising the steps of:
(1) Preparing a fermentation basic culture medium, wherein the formula comprises the following components: 2.5 to 6.0 percent of soybean cake powder, 0.05 to 0.1 percent of monopotassium phosphate, 0.1 to 0.5 percent of sodium chloride, 0.5 to 1.0 percent of corn oil, 0.5 to 1.0 percent of corn steep liquor dry powder, 0.015 to 0.025 percent of polyoxypropylene polyoxyethylene glyceryl ether GPE, tap water for volume fixation, pH: naturally; preparing a feed supplement culture medium, namely preparing glucose powder into a glucose solution, wherein the mass percentage of the glucose powder is 0.5% -1.0%; then adding sodium citrate into the glucose solution, wherein the mass percent of the sodium citrate is 0.15-0.25%, and then adding 0.15-0.25% ammonia water to adjust the pH value;
(2) And (3) sterilization: the fermentation basic culture medium is maintained for 30min at the temperature of 121-130 ℃; the feed supplement culture medium is maintained at 110-120 ℃ for 30min;
(3) Inoculating, cooling to 28-30 ℃ after sterilization, and inoculating streptomyces parviflorus with the inoculum size of 5-15vol%;
(4) Fermentation conditions: temperature: 28-30 ℃, the aeration ratio is 1:0.5-1:1.5, the stirring rotation speed is 150-350rpm, the tank pressure is 0.03-0.05MPa, and the fermentation period is 160-170h; after fermentation, collecting fermentation products to obtain fermentation filtrate;
(5) Fermentation feeding conditions: fermenting and culturing for 20-30 hours, and continuously adding glucose and sodium citrate mixed solution and ammonia water when the pH is reduced to 6.8-7.3, and maintaining the pH between 6.8-7.3.
4. A fermentation process according to claim 3, wherein in step (1), the mass concentration of ammonia is 25% for pH adjustment.
5. A fermentation process according to claim 3, wherein the inoculum size is 15%.
6. A fermentation process according to claim 3, wherein sodium citrate is added to the kasugamycin feed medium in an amount of 0.20% to 0.25%.
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