CN113826629B - Application of piperlongumine in preventing and treating plant pathogenic fungi - Google Patents

Application of piperlongumine in preventing and treating plant pathogenic fungi Download PDF

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CN113826629B
CN113826629B CN202111143459.3A CN202111143459A CN113826629B CN 113826629 B CN113826629 B CN 113826629B CN 202111143459 A CN202111143459 A CN 202111143459A CN 113826629 B CN113826629 B CN 113826629B
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piperlongumine
pathogenic fungi
plant pathogenic
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treatment
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CN113826629A (en
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冯岗
张静
朱发娣
叶火春
闫超
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CATAS Environment and Plant Protection Institute
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/34—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom
    • A01N43/40—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one nitrogen atom as the only ring hetero atom six-membered rings
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

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  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
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  • Engineering & Computer Science (AREA)
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  • General Health & Medical Sciences (AREA)
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Abstract

The application of the piperlongumine in the prevention and treatment of plant pathogenic fungi is provided by the invention, the test concentration of the piperlongumine serving as a bactericide for the prevention and treatment of the plant pathogenic fungi is more than or equal to 200mg/L, and the piperlongumine serving as a bacteriostatic active ingredient can be used for preventing and treating blight fungus, gray mold fungus, gibberellic disease, ring rot, ring spot, brown spot, downy blight fungus, big spot and corynespora leaf drop fungus, the inhibition rate is more than 80%, and meanwhile, the piperlongumine also has strong protection and treatment effects on tomato botrytis cinerea. Experiments show that the piperlongumine has broad-spectrum bactericidal effect on plant pathogenic fungi, and lays a foundation for further creating a novel botanical fungicide by using piper longum of the family Piperaceae.

Description

Application of piperlongumine in prevention and treatment of plant pathogenic fungi
Technical Field
The invention relates to the technical field of application of botanical fungicides, in particular to application of piperlongumine in prevention and treatment of plant pathogenic fungi.
Background
Piperlongumine (Piperlongumine), also called piperlongumine, belongs to amide alkaloid compounds, and has a chemical structure shown as the following, and a molecular formula C14H19NO5Relative molecular mass 317.3410 is mainly present in root of Piper longum of Piperaceae.
In recent years, with the continuous and intensive research, the piperlongumine has important medicinal value, and can be used for treating tumors, spleen diseases, chronic malaria, viral hepatitis, cough, stomachache, asthma, bronchitis, diarrhea, respiratory tract infection, tongue paralysis, gonorrhea and the like. In addition, the researches in recent years also find that the piperlongumine has the effects of resisting anxiety, vascular proliferation, platelet aggregation, inflammation, tumor and the like, and is a chemical substance with great medicinal value. At present, the research on the anti-tumor activity of the piperlongumine is the most extensive, and the application of the piperlongumine compound in the aspect of preventing and treating plant pathogenic fungi is not reported. Therefore, the research on the field of the prevention and the treatment of the plant pathogenic fungi of the piperlongumine is further facilitated, and the application value of the piperlongumine is fully exerted.
Figure BDA0003284531110000011
Chemical structure of piperlongumine
Disclosure of Invention
In view of the above, the invention provides the application of the piperlongumine in the prevention and treatment of plant pathogenic fungi, the piperlongumine has strong agricultural bactericidal activity, has strong bacteriostatic action on botrytis cinerea, wheat scab, tobacco brown spot and peronophythora litchi at the test concentration of 200ug/L, and has the inhibition rate of more than 80%.
The technical scheme of the invention is realized as follows:
the invention provides application of piperlongumine in prevention and treatment of plant pathogenic fungi, and application of piperlongumine serving as a bactericide or a bacteriostatic agent in prevention and treatment of plant pathogenic fungi.
Further, the concentration of the piperlongumine used as a bactericide for preventing and treating plant pathogenic fungi is more than or equal to 200mg/L.
Further, the piperlongumine is used as an antibacterial active ingredient for preventing and treating at least one of fusarium wilt disease, gray mold disease, fusarium graminearum disease, ring spot disease, brown spot disease, downy mildew disease, northern leaf spot disease and corynespora deciduous leaf disease.
Further explaining, the application of the piperlongumine as an antibacterial active ingredient in the aspect of preventing and treating Fusarium oxysporum f.sp cube.
Further explaining, the application of the piperlongumine as an antibacterial active ingredient in the aspect of preventing and treating Botrytis cinerea (Botrytis cinerea).
Further illustrates the application of the piperlongumine in the prevention and treatment of Botrytis cinerea (Botrytis cinerea).
Further explaining, the application of piperlongumine as an antibacterial active ingredient in the aspect of preventing and treating wheat scab (Fusarium graminearum).
Further explaining, the application of the piperlongumine as an antibacterial active ingredient in the aspect of preventing and treating ring rot fungus (Thanatephorus cucumeri) is disclosed.
Further illustrates the application of piperlongumine as an antibacterial active ingredient in the aspect of preventing and treating Alternaria alternate (Alternaria alternata).
Further illustrates the application of the piperlongumine as an antibacterial active ingredient in the aspect of preventing and controlling phytophthora litchi (Peronophytoora litchii).
Further illustrates the application of piperlongumine as an antibacterial active ingredient in the aspect of preventing and controlling northern leaf blight (Exserohilum turcicum) and Corynespora caerulea deciduous (Corynespora cassicola).
More preferably, the piperlongumine is used as an antibacterial active ingredient, an auxiliary agent is selected to prepare a bactericide or a bacteriostatic agent, and according to the actual needs, the piperlongumine can be prepared into a suspending agent, an aqueous emulsion, a microemulsion, missible oil, wettable powder or a water dispersible granule by adopting a conventional method in the field.
The Piperlongumine can be obtained by chemical synthesis or separation from Piperlongum root, and has white powder, CAS number of 20069-09-4, and molecular formula of C17H19NO5The molecular weight is 317.34.
Compared with the prior art, the invention has the beneficial effects that:
the application of the piperlongumine in the prevention and treatment of plant pathogenic fungi, disclosed by the invention, is proved by experimental research that the piperlongumine has a remarkable inhibitory effect on the plant pathogenic fungi, and a natural source bactericidal active substance is provided for the prevention and treatment of agricultural diseases; the piperlongumine can be used as an antibacterial active ingredient for preventing and treating a plurality of important agricultural diseases such as fusarium wilt, gray mold, gibberellic disease, ring spot, brown spot, downy blight, northern leaf spot, corynespora deciduous leaf disease and the like, has the characteristics of high efficiency, low toxicity, safety and the like as a bactericide, meets the requirements of creating a new pesticide at present, and fully exerts the application value of the piperlongumine in the technical field of a material source bactericide.
In order to evaluate the agricultural bactericidal activity of the piperlongumine, the invention adopts a growth rate method to measure the inhibitory action of the compound on plant pathogenic fungi, evaluates the bacteriostatic activity of the compound on the plant pathogenic fungi, and further verifies the bacteriostatic effect of the compound by a tissue method. Tests prove that the piperlongumine has strong bacteriostatic action on botrytis cinerea, fusarium graminearum and tobacco anthracnose pathogen when the tested concentration is 200mg/L, and the inhibition rate is 90-94%; the litchi downy mildew has a certain bacteriostatic action, and the inhibition rate is 82.56%; meanwhile, the results of a tissue method test show that the piperlongumine has a strong protection effect on botrytis cinerea, and when the test concentration is 500mg/L and 250mg/L, the protection effects are respectively 81.80% and 77.95%, and are remarkably superior to that of a control medicament iprodione (71.86%); but also has stronger treatment effect on botrytis cinerea, but the strength of the botrytis cinerea is lower than that of the botrytis cinerea, and the treatment effect is 70.68% when the test concentration is 250mg/L, and has no significant difference with a control medicament namely iprodione (67.66%).
Detailed Description
In order to better understand the technical content of the invention, specific examples are provided below to further illustrate the invention.
The experimental methods used in the examples of the present invention are all conventional methods unless otherwise specified.
The materials, reagents and the like used in the examples of the present invention are commercially available unless otherwise specified.
In the research on agricultural bactericidal active substances of the piperlongumine, the activity tracking method is adopted to discover that the piperlongumine has the control effect on plant pathogenic bacteria. The following experiments are taken as examples to verify the effect of piperlongumine in controlling plant diseases.
Example 1 application of Piperlongumine for controlling plant diseases
1. Sources of Piperlongumine
Piperlongumine, CAS number 20069-09-4, purity greater than 98%, purchased from Gooduet bioscience, inc.
2. Determination of inhibitory Activity of Piperlongumine against plant pathogenic fungi
(1) Test strains
Banana wilt pathogen (Fusarium oxysporum f.sp. Cuben), botrytis cinerea (Botrytis cinerea), fusarium graminearum (Fusarium graminearum), verticillium graminearum (Fusarium graminearum), apple ring spot pathogen (Thanatephorus cuprimeris), alternaria alternata (Alternaria alternata), rhizoctonia solani (rhizoctonia solani), peronospora litchii (peronophythora litchii), alternaria turcicola (Exserohilum), corynebacterium hevaria (corynebacterium), corynespora cassiae (Corynespora cassiicola), all of which are provided by the group of pesticide chemistry and biology and application subjects of the institute of environmental and plant protection in tropical agrology in china.
(2) Test method
(1) In vitro Activity assay
For the agricultural bactericidal activity of the piperlongumine, the growth rate method is adopted to evaluate the hypha growth inhibition effect of the compound on plant pathogenic fungi. The method for inhibiting the growth rate of hypha is adopted to measure the inhibitory activity of the piperlongumine to the plant pathogenic fungi, and is referred to as the method of Zhongda (Fangzhouda. Plant disease research method, chinese agriculture press). And hyphal growth was carefully observed and recorded under an inverted microscope (400X).
The method for determining the growth inhibition effect of the piperlongumine on the hypha of the plant pathogenic fungi takes 200mg/L and 100mg/L as screening concentrations, and a tissue method determination test is carried out on the pathogenic fungi with higher expression inhibition activity so as to verify the activity intensity of the pathogenic fungi. Each treatment was repeated 3 times (3 dishes) with blank medium as control.
Hypha growth inhibition (%) = (control colony diameter-treated colony diameter control colony diameter)/control colony diameter × 100%
(2) Data analysis
The results of the bacteriostatic activity assay of piperlongumine against the plant pathogenic fungi of test 9 are shown in table 1,
TABLE 1 bacteriostatic activity of Piperlongumine against 9 phytopathogenic fungi
Figure BDA0003284531110000051
The test results in the table show that when the tested concentration of the piperlongumine is 200mg/L, the piperlongumine has strong bacteriostatic action on botrytis cinerea, fusarium graminearum and tobacco anthracnose, and the inhibition rate is 90-94%; the bacteriostatic agent also has a certain bacteriostatic action on phytophthora litchi, and the inhibition rate of the bacteriostatic agent can reach 82.56%.
Example 2 potting test for tomato Gray mold
The invention takes Piperlongumine as an antibacterial active ingredient to carry out a living pot experiment of botrytis cinerea, and the experiment is started when 3-5 true leaves grow out after tomato seeds are sown in a flowerpot after germination acceleration by slightly improving a test method reference (Liqian, wanhong Wang, et al. The protection and treatment effects are respectively set to be 3 concentration treatments, namely 500, 250 and 125mg/L, each treatment is repeated for 3 times, each treatment is carried out in 3 basins, meanwhile, 50% iprodione wettable powder (200 mg/L) is set to be a medicament control, and clear water is set to be a blank control.
(1) The method for measuring the protection effect comprises the following steps: spraying test liquid medicine, and inoculating the tomato botrytis cinerea spore suspension after 24 h.
(2) The method for determining the treatment effect comprises the following steps: inoculating the botrytis cinerea spore suspension under the condition of moisture preservation, and spraying the test liquid medicine after 24 hours.
The treated tomato seedlings are cultured under the conditions of (18 +/-1) DEG C, saturation humidity and illumination/darkness =12h/12h, and the disease index and the prevention and treatment effect are calculated according to the investigation result after 7 days.
Grading leaf diseases [ refer to pesticide effect I, prevention and treatment of vegetable gray mold with bactericide ]:
stage 0: no disease spots;
stage 1: the lesion area accounts for less than 5% of the whole leaf area;
and 3, level: the lesion area accounts for 6-15% of the whole leaf area;
and 5, stage: the lesion area accounts for 16-25% of the whole leaf area;
and 7, stage: the lesion area accounts for 26-50% of the whole leaf area;
and 9, stage: the lesion area accounts for more than 51 percent of the whole leaf area;
the drug effect calculation formula is as follows:
disease index = (number of diseased leaves at each stage × relative stage value)/(total investigated leaves number × highest disease value) × 100
Control effect (%) = (control disease index-treatment disease index)/control disease index x 100
(3) The measurement results are shown in table 2 below:
Figure BDA0003284531110000061
according to the test results of the tissue method in the table 2, the piperlongumine has strong protective effect on botrytis cinerea, and when the tested concentrations of the piperlongumine are 500mg/L and 250mg/L, the protective effect on tomato seedlings is 81.80 percent and 77.95 percent respectively, and the piperlongumine is obviously superior to that of a control medicament namely iprodione (71.86 percent); moreover, the composition also has a strong treatment effect on botrytis cinerea, but the intensity of the composition is lower than that of the protection effect, and the treatment effect is 70.68% at the test concentration of 250mg/L, and has no significant difference with a control medicament namely iprodione (67.66%).
In conclusion, the piperlongumine has a remarkable inhibiting effect on plant pathogenic fungi, can be used as an antibacterial active ingredient for preventing and treating a plurality of important agricultural diseases such as fusarium wilt, gray mold, gibberellic disease, ring rot, brown spot, downy blight, big spot, corynespora deciduous leaf disease and the like, has the characteristics of high efficiency, low toxicity, safety and the like when being used as a bactericide, and provides a natural source bactericidal active substance for preventing and treating the agricultural diseases; the application value of the piperlongumine in the technical field of the source bactericide is fully exerted.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.

Claims (1)

1. The application of the piperlongumine in preventing and treating plant pathogenic fungi is characterized in that: the piperlongumine is used as an antibacterial active component and is used for preventing and treating at least one of banana wilt pathogen, tomato botrytis cinerea, wheat scab pathogen, apple ring spot pathogen, tobacco brown spot pathogen, phytophthora litchi, corn northern leaf blight and rubber tree corynespora deciduous leaf pathogen; the concentration of the piperlongumine used as a bactericide for preventing and treating plant pathogenic fungi is more than or equal to 200mg/L.
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