CN113820906A - Indoor root system phenotype detection platform - Google Patents
Indoor root system phenotype detection platform Download PDFInfo
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- CN113820906A CN113820906A CN202111147309.XA CN202111147309A CN113820906A CN 113820906 A CN113820906 A CN 113820906A CN 202111147309 A CN202111147309 A CN 202111147309A CN 113820906 A CN113820906 A CN 113820906A
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Abstract
The invention discloses an indoor root phenotype detection platform which comprises a root chamber, wherein a root box array consisting of root box units is arranged in the root chamber, and the root box units form root box unit columns by taking columns as groups; a track is arranged between the root box unit rows, a walking carrier is arranged on the track, and an imaging acquisition unit is arranged on the walking carrier; one side of the glass plate of the root box unit array inclines downwards and faces the track, the other side of the root box unit array is provided with a black curtain, and the black curtain is controlled by an electric roller shutter above the black curtain. The invention provides a practical, efficient and systematic indoor root phenotype detection platform, wherein a black curtain is added in a root chamber and is controlled by an electric roller shutter above the black curtain, so that background noise during photographing can be effectively removed.
Description
Technical Field
The invention relates to the technical field of root phenotype detection, in particular to an indoor root phenotype detection platform.
Background
The roots, known as the "hidden half," grow mainly underground and are important organs of the plant. The root system can obtain water and nutrients from the soil and is also a storage place for the nutrients. The root system configuration determines the absorption capacity of the crops to water and nutrients in soil, and the phenotypic change of the overground part and the yield. The developed root system is the most obvious and important characteristic for the plants to respond to the stress. The improvement of root system characters is beneficial to molding an ideal whole plant type with unified ground and underground characters, thereby breaking the bottleneck of single breeding of the ground characters.
The method and the device for acquiring the plant root system data are always the key points of root system research and mainly divided into two categories of indoor detection and field detection. The traditional method for manually acquiring the phenotype data has low efficiency, low precision and high cost, and although the root system configuration detection method is continuously developed in recent years, the method is limited by environmental conditions, technical means and the like, high-flux and accurate measurement is difficult to realize within a long period of time, and the crop root system cannot be directly improved. A root box type root system phenotype detection system is a novel planting method which is developed in recent years, plants can be directly planted in soil, various root system related parameters such as root length, root width, root depth and convex hull area are obtained through a carried hyperspectral camera and an RGB visible light camera, and the root box type root system phenotype detection system can be applied to research on various crops such as rice, wheat, corn, soybean and rape.
But the root box planting mode is not developed soon, and the system is far from mature. The existing root box is mainly used for outdoor cultivation, cannot accurately regulate and control temperature, humidity, illumination and the like, and cannot meet the requirement of accurate agriculture.
The development of indoor cultivation mode has the following problems:
firstly, a matched denoising system is lacked, and a high-definition image is difficult to obtain due to the problems of insufficient indoor light, local over-strong indoor light, large background noise and the like.
Secondly, the existing robot imaging system is not mature enough, and can only collect the side-looking photos and the root system photos of the limited angles of the overground part, and multi-angle overlooking and side-looking shooting cannot be achieved. Moreover, the collection of the overhead top view photos cannot be realized all the time, the complete, comprehensive and real plant growth state is difficult to obtain, and for different species, partial characters obtained from the top view angle can be important references for the whole plant types of some species.
Thirdly, with the development of high-throughput omics, due to the limitation of the size of the root box, the flux and the application of root system research are restricted by the single-plant planting and cultivating mode of the root box, and the important problem of setting biological repetition in the root box as much as possible so as to shorten the research time of the root system is faced by the device. Meanwhile, if the double-plant cultivation is directly carried out, different biological repetition or cross influence between experimental material roots can be generated. Particularly, in some adversity biology experiments, such as drought and salt treatment, a control group is required to grow normally, an experimental group is subjected to corresponding drought stress or salt stress, and the existing root box cannot meet the requirement that the control group and the experimental group are planted in the same root box at the same time, so that the development of root box application and scientific research is limited.
Based on the problems, a set of systematic, complete and mature indoor root phenotype detection platform needs to be established, and a new thought and reference is provided for analyzing the genetic basis of crop roots and carrying out genetic improvement and molecular breeding on the crop roots by using a high-throughput phenotype group technology.
Disclosure of Invention
The present invention is directed to solving, at least to some extent, one of the technical problems in the related art.
Therefore, the invention provides a practical, efficient and systematic indoor root phenotype detection platform.
The invention adopts the following technical scheme:
an indoor root phenotype detection platform comprises a root chamber, wherein a root box array consisting of root box units is arranged in the root chamber, and the root box units form root box unit rows by taking rows as groups; a track is arranged between the root box unit rows, a walking carrier is arranged on the track, and an imaging acquisition unit is arranged on the walking carrier; one side of the glass plate of the root box unit array inclines downwards and faces the track, the other side of the root box unit array is provided with a black curtain, and the black curtain is controlled by an electric roller shutter above the black curtain.
Further, the imaging acquisition unit comprises a root system imaging acquisition unit and a crop overground part imaging acquisition unit, a support extending upwards is installed on the walking carrier, the root system imaging acquisition unit is installed on the walking carrier, and the crop overground part imaging acquisition unit is installed on the support.
Furthermore, the above-ground crop imaging acquisition unit comprises a vertical rod, and a first camera and a first light supplement lamp are mounted on the vertical rod, wherein the light supplement direction of the first light supplement lamp horizontally points to the root box unit;
the top of montant is connected with the horizon bar, installs second camera and second light filling lamp on the horizon bar, and wherein, the perpendicular downward directional root box unit of second light filling lamp light filling direction arranges that the direction of shooing of second camera is perpendicular downward directional root box unit arranges.
Further, the vertical rod is rotatably connected with the top end of the support; the support is a telescopic support which can be telescopic in the up-down direction.
Further, the root box unit comprises a root box and a main frame, wherein the root box is obliquely placed on the main frame; the root box comprises a soil containing frame, cover plates and glass plates, wherein the cover plates and the glass plates are positioned on the front side and the rear side of the soil containing frame, and a soil containing cavity is formed between the soil containing frame and the cover plates and between the cover plates and the glass plates.
Further, the root box unit is provided with a partition board in the soil loading cavity, the partition board extends along the length direction of the soil loading cavity, and the partition board divides the soil loading cavity into a plurality of sub-cavities.
Furthermore, the partition plate comprises a front partition plate strip and a rear partition plate strip, and the front partition plate strip and the rear partition plate strip are connected through an elastic viscose layer in the middle; the front partition lath and the rear partition lath are respectively in sealing pressure joint with the corresponding cover plate and the glass plate under the elastic action of the elastic adhesive layer.
Furthermore, scales are arranged on the front partition lath and/or the rear partition lath along the length direction.
Furthermore, a light supplementing unit for the root box is arranged on the main frame of the root box unit.
Furthermore, an indoor root box drip irrigation system, a temperature management system, a humidity management system and an illumination management system are arranged in cooperation with the root box unit.
The invention has the beneficial effects that:
1. according to the invention, the black curtain is added in the root chamber and is controlled by the electric roller shutter above the black curtain, so that background noise during photographing can be effectively removed.
The light supplementing unit is arranged on the main frame, and is matched with the black curtain, so that image background noise is further effectively eliminated.
2. The imaging acquisition unit comprises a root system imaging acquisition unit and a crop overground part imaging acquisition unit, wherein the crop overground part imaging acquisition unit is arranged on the bracket and can acquire clear side-view, top-view and root system pictures of the overground part of the plant, so that the acquisition of the overground part top-view picture is realized, the complete, comprehensive and real plant growth state is obtained, and the imaging acquisition unit is suitable for researching the characteristics of the whole plant of the plant.
According to the invention, the vertical rod is rotatably connected with the top end of the support, and the support is a telescopic support in the vertical direction, so that the vertical stretching and the left-right rotation of the imaging acquisition unit on the overground part of the crop are realized, and the device is suitable for different crop researches and multi-angle overlooking and side-looking shooting.
The support is telescopic and can be stored, so that the whole set of device is more portable.
3. According to the invention, the soil containing cavity is divided into a plurality of sub-cavities by the partition plates, so that double-plant or multi-plant planting is realized, the flux of the root box is effectively improved, the root system research time is shortened, the use of the root box is expanded, and various scientific research and production requirements are met.
The partition plate comprises a front partition plate strip and a rear partition plate strip, wherein the front partition plate strip and the rear partition plate strip are connected through an elastic viscose layer in the middle; preceding division lath and back division lath are sealed the crimping with the apron that corresponds and glass board respectively under the elasticity effect on elasticity viscose layer, and the baffle can effectively completely cut off root box both sides space, and this material repeatedly usable is not fragile, and elasticity viscose layer helps abundant airtight space gap.
The scales are arranged on the front partition lath and/or the rear partition lath along the length direction, so that experimental observation and recording are facilitated.
4. The invention realizes accurate agriculture by combining stable and controllable temperature, humidity and illumination management through an equipped indoor root box drip irrigation system.
Drawings
FIG. 1 is a schematic view of the overall arrangement of the present invention;
FIG. 2 is a front view of FIG. 1;
FIG. 3 is a first perspective view of an imaging acquisition unit according to the present invention;
FIG. 4 is a second perspective view of an imaging acquisition unit according to the present invention;
FIG. 5 is a front view of an imaging acquisition unit portion of the present invention;
FIG. 6 is a schematic view of the construction of the root box unit according to the present invention;
FIG. 7 is a longitudinal cross-sectional view of the root box of the present invention at the spacer;
fig. 8 is a longitudinal sectional view of the separator in the present invention.
The drawings are for illustrative purposes only and are not to be construed as limiting the patent; for the purpose of better illustrating the embodiments, certain features of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted.
Description of the preferred embodiments
The technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.
As shown in fig. 1 to 8, the present embodiment provides an indoor root phenotype detection platform, which includes a root chamber, a root box array formed by root box units 1 is arranged in the root chamber, and the root box units 1 form a root box unit column 5 by columns; a track 2 is arranged between the root box unit columns 5, a walking carrier 3 is arranged on the track 2, and an imaging acquisition unit is arranged on the walking carrier 3.
In this embodiment, the root cartridge unit 1 includes a root cartridge 11 and a main frame 12, the root cartridge 11 being placed on the main frame 12 obliquely; the root box 11 comprises an earth holding frame 13, and a cover plate 14 and a glass plate 15 which are positioned at the front side and the rear side of the earth holding frame 13, wherein an earth holding cavity is formed between the earth holding frame 13, the cover plate 14 and the glass plate 15.
One side of the glass plate of the root box unit row 5 inclines downwards and faces the track 2, the other side of the root box unit row 5 is provided with a black curtain 4, and the black curtain 4 is controlled by an electric roller shutter 41 above the black curtain 4, so that background noise during photographing can be effectively removed.
In this embodiment, the main frame 12 is provided with the light supplement unit 13 for the root box 11, the light supplement unit 13 includes a lamp tube, the lamp tube is fixed on two sides of the root box 11, and the lamp tube is fixed with an angle aluminum sprayed with black matte paint for blocking light in the opposite direction, so as to solve the problems of uneven light and light reflection of the root box glass from the root. Meanwhile, the light supplementing unit is matched with the black curtain, so that image background noise is further effectively eliminated.
The imaging acquisition unit comprises a root imaging acquisition unit 31 and a crop overground part imaging acquisition unit 32, a support 33 extending upwards is mounted on the walking carrier 3, and the support 33 is a telescopic support capable of being telescopic in the vertical direction. The support 33 adopts a tripod structure, can be extended and retracted, and enables the whole device to be more portable.
The root imaging acquisition unit 31 is installed on the walking carrier 3, and the crop overground part imaging acquisition unit 32 is installed on the bracket 33.
The crop overground part imaging acquisition unit 32 comprises a vertical rod 34, the vertical rod 34 is rotatably connected with the top end of the support 33, and a first camera 35 and a first light supplement lamp 36 are mounted on the vertical rod 34. The light supplement direction of the first light supplement lamp 36 points to the root box unit horizontally, and the photographing direction of the first camera 35 points to the root box unit horizontally.
The top end of the vertical rod 34 is connected with a horizontal rod 37, and a second camera 38 and a second light supplement lamp 39 are installed on the horizontal rod 37. The light supplement direction of the second light supplement lamp 39 is vertically downward and points to the root box unit, and the photographing direction of the second camera 38 is vertically downward and points to the root box unit.
The components and parts adopted by the root system imaging acquisition unit 31 and the crop overground part imaging acquisition unit 32 are all the prior art.
The imaging acquisition unit comprises a root system imaging acquisition unit 31 and a crop overground part imaging acquisition unit 32, wherein the crop overground part imaging acquisition unit 32 is arranged on the bracket and can acquire clear side-view, top-view and root system pictures of the overground part of the plant, so that the acquisition of the overground part top-view pictures is realized, the complete, comprehensive and real plant growth state is obtained, and the imaging acquisition unit is suitable for researching the characteristics of the whole plant of the plant.
The invention can also realize the up-and-down stretching and the left-and-right rotation of the crop overground part imaging acquisition unit 32, and is suitable for different crop researches and multi-angle overlooking and side-looking shooting.
Arrange about the dress soil intracavity of root box unit 1 and be equipped with a plurality of baffle 6, every baffle 6 extends along the length direction who adorns the soil chamber, and baffle 6 will adorn the soil chamber and control to separate for a plurality of sub-chamber, and this embodiment is preferred, and baffle 6 is equipped with one, and baffle 6 will adorn the soil chamber and control to separate for two sub-chambers, forms two plants and plants, effectively improves root box flux, shortens root system research time, extends root box usage, satisfies multiple scientific research and production needs.
The partition board 6 comprises a front partition board strip 61 and a rear partition board strip 62, wherein the front partition board strip 61 and the rear partition board strip 62 are connected through an elastic adhesive layer 63 in the middle; preceding division lath 61 and back division lath 62 are sealed the crimping with corresponding apron 14 and glass board 15 respectively under the elastic action of elasticity viscose layer 63, and baffle 6 can effectively completely cut off root box both sides space, repeatedly usable, and elasticity viscose layer 63's elastic action helps abundant airtight space gap.
In this embodiment, the front partition plate 61 and the rear partition plate 62 are made of PVC, and are not easily damaged and can be reused.
In this embodiment, the front partition plate strip 61 and/or the rear partition plate strip 62 are provided with scales along the length direction, so as to facilitate experimental observation and recording.
Still be equipped with indoor root box drip irrigation system with the cooperation of root box unit 1 to and temperature management system, humidity management system and illumination management system realize stable controllable temperature, humidity, illumination management, realize accurate agricultural.
The embodiment establishes a systematic, complete and mature indoor root phenotype detection platform, and provides a new idea and reference for analyzing the genetic basis of the crop root and carrying out genetic improvement and molecular breeding on the crop root by using a high-throughput phenotype group technology.
Although the present invention has been described in detail with reference to the above embodiments, it should be understood by those skilled in the art that: modifications and equivalents may be made thereto without departing from the spirit and scope of the invention and it is intended to cover in the claims the invention as defined in the appended claims.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", and the like indicate orientations or positional relationships based on those shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the scope of the present invention.
Claims (10)
1. An indoor root phenotype detection platform comprises a root chamber, wherein a root box array consisting of root box units is arranged in the root chamber, and the root box units form root box unit rows by taking rows as groups; a track is arranged between the root box unit rows, a walking carrier is arranged on the track, and an imaging acquisition unit is arranged on the walking carrier; the glass plate one side of root box unit row inclines downwards and faces the track, its characterized in that: the other side of root box unit row is equipped with the black curtain, and the black curtain is by the electronic book curtain control of top.
2. The indoor root phenotype detection platform of claim 1, wherein: the imaging acquisition unit comprises a root system imaging acquisition unit and a crop overground part imaging acquisition unit, a support extending upwards is installed on the walking carrier, the root system imaging acquisition unit is installed on the walking carrier, and the crop overground part imaging acquisition unit is installed on the support.
3. The indoor root phenotype detection platform of claim 2, wherein: the crop overground part imaging acquisition unit comprises a vertical rod, and a first camera and a first light supplement lamp are mounted on the vertical rod, wherein the light supplement direction of the first light supplement lamp points to the root box unit horizontally, and the photographing direction of the first camera points to the root box unit horizontally;
the top of montant is connected with the horizon bar, installs second camera and second light filling lamp on the horizon bar, and wherein, the perpendicular downward directional root box unit of second light filling lamp light filling direction arranges that the direction of shooing of second camera is perpendicular downward directional root box unit arranges.
4. The indoor root phenotype detection platform of claim 3, wherein: the vertical rod is rotatably connected with the top end of the support; the support is a telescopic support which can be telescopic in the up-down direction.
5. The indoor root phenotype detection platform of claim 1, wherein: the root box unit comprises a root box and a main frame, and the root box is obliquely placed on the main frame; the root box comprises a soil containing frame, cover plates and glass plates, wherein the cover plates and the glass plates are positioned on the front side and the rear side of the soil containing frame, and a soil containing cavity is formed between the soil containing frame and the cover plates and between the cover plates and the glass plates.
6. The indoor root phenotype detection platform of claim 5, wherein: the root box unit is provided with a partition board in the soil loading cavity, the partition board extends along the length direction of the soil loading cavity, and the partition board divides the soil loading cavity into a plurality of sub-cavities.
7. The indoor root phenotype detection platform of claim 6, wherein: the partition plate comprises a front partition plate strip and a rear partition plate strip, and the front partition plate strip and the rear partition plate strip are connected through an elastic viscose layer in the middle; the front partition lath and the rear partition lath are respectively in sealing pressure joint with the corresponding cover plate and the glass plate under the elastic action of the elastic adhesive layer.
8. The indoor root phenotype detection platform of claim 7, wherein: scales are arranged on the front partition lath and/or the rear partition lath along the length direction.
9. The indoor root phenotype detection platform of claim 5, wherein: and a light supplementing unit for the root box is arranged on the main frame of the root box unit.
10. The indoor root phenotype detection platform of any one of claims 1-9, wherein: an indoor root box drip irrigation system, a temperature management system, a humidity management system and an illumination management system are arranged in cooperation with the root box units.
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