CN113813903B - Multichannel polypeptide reaction synthesizer - Google Patents
Multichannel polypeptide reaction synthesizer Download PDFInfo
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- CN113813903B CN113813903B CN202111075260.1A CN202111075260A CN113813903B CN 113813903 B CN113813903 B CN 113813903B CN 202111075260 A CN202111075260 A CN 202111075260A CN 113813903 B CN113813903 B CN 113813903B
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- 229920001184 polypeptide Polymers 0.000 title claims abstract description 78
- 102000004196 processed proteins & peptides Human genes 0.000 title claims abstract description 78
- 108090000765 processed proteins & peptides Proteins 0.000 title claims abstract description 78
- 238000006243 chemical reaction Methods 0.000 title claims abstract description 66
- 238000002347 injection Methods 0.000 claims abstract description 81
- 239000007924 injection Substances 0.000 claims abstract description 81
- 239000012295 chemical reaction liquid Substances 0.000 claims abstract description 48
- 239000007788 liquid Substances 0.000 claims abstract description 28
- 238000003756 stirring Methods 0.000 claims description 54
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 41
- 239000000243 solution Substances 0.000 claims description 37
- 229910052757 nitrogen Inorganic materials 0.000 claims description 18
- 238000005273 aeration Methods 0.000 claims description 10
- 238000010438 heat treatment Methods 0.000 claims description 9
- 238000005485 electric heating Methods 0.000 claims description 8
- 238000009434 installation Methods 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 6
- 229910001873 dinitrogen Inorganic materials 0.000 claims description 5
- 230000005540 biological transmission Effects 0.000 claims description 4
- 230000002457 bidirectional effect Effects 0.000 claims description 2
- 238000012545 processing Methods 0.000 abstract description 36
- 239000003153 chemical reaction reagent Substances 0.000 description 8
- 239000007790 solid phase Substances 0.000 description 7
- 244000309464 bull Species 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000000712 assembly Effects 0.000 description 1
- 238000000429 assembly Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000010511 deprotection reaction Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 238000010532 solid phase synthesis reaction Methods 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0006—Controlling or regulating processes
- B01J19/0013—Controlling the temperature of the process
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/18—Stationary reactors having moving elements inside
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J4/00—Feed or outlet devices; Feed or outlet control devices
- B01J4/001—Feed or outlet devices as such, e.g. feeding tubes
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Peptides Or Proteins (AREA)
Abstract
The invention relates to the technical field of polypeptide synthesizers, in particular to a multichannel polypeptide reaction synthesizer which comprises a synthesizer body, wherein a channel is arranged at the top end of the synthesizer body, a plurality of outer tubes are arranged at the top end of the channel, injection tubes are connected in each outer tube, and injection needles are arranged at the bottom end of each injection tube. According to the invention, the first piston is pushed downwards to add the reaction liquid in the injection tube into the channel and the synthesizer body through the liquid discharge tube and the injection needle, and the moving distance of the first piston can be visually observed through the scale marks on the first piston rod, so that the adding quantity of the reaction liquid can be accurately controlled, the reaction liquid in the reaction liquid storage tank can be sucked into the injection tube through the liquid suction tube and the connecting tube by pulling upwards, the reaction liquid can be conveniently and continuously added into the injection tube without mixing air, the operation is convenient, and the processing quality of the multichannel polypeptide reaction synthesizer is improved.
Description
Technical Field
The invention relates to the technical field of polypeptide synthesizers, in particular to a multichannel polypeptide reaction synthesizer.
Background
In the prior art, in the polypeptide solid-phase synthesis reaction, an activating agent and a deprotection agent are needed to be alternately used as reaction liquids to participate in the reaction, and the two reaction liquids are needed to be alternately added for a plurality of times.
Through retrieval, chinese patent No. CN208883754U discloses a semiautomatic multi-channel solid-phase polypeptide synthesizer, which comprises a multi-channel solid-phase polypeptide synthesizer, the upper end of the multi-channel solid-phase polypeptide synthesizer is provided with an upper through hole, the upper through hole penetrates through an internal reaction channel arranged on the multi-channel solid-phase polypeptide synthesizer, the upper through hole is fixedly connected with a channel, the lower end of the channel is fixedly connected with a first sealing ring with the upper through hole, the inner side of the channel is movably connected with a drain pan, the lower end of the drain pan is movably provided with a micro motor, the upper end of the channel is provided with two slotted holes, and a first reagent tube and a second reagent tube are respectively inserted in the two slotted holes.
According to the semiautomatic multi-channel solid-phase polypeptide synthesizer, although the two reagent pipes are connected to the upper end of the multi-channel solid-phase polypeptide synthesizer, the two reagent pipes are poured into corresponding reaction liquid to be sealed, and in the synthesis process, the two different valves are used for alternately controlling operation, so that compared with the traditional method of gradually pouring, the semiautomatic multi-channel solid-phase polypeptide synthesizer is time-saving and labor-saving, more accurate, and the purity of reactants is improved, but the following defects still exist:
1. the reaction liquid in the first reagent tube and the second reagent tube is added into the polypeptide synthesizer through the control valve, so that the quantity of the added reaction liquid cannot be accurately controlled, and air is easy to mix into the polypeptide synthesizer when the reaction liquid is added from the top ends of the first reagent tube and the second reagent tube, thereby causing the processing quality of the polypeptide synthesizer to be poor;
2. the miniature motor is arranged inside the channel, so that the installation, the disassembly and the maintenance are inconvenient, and the motor is easy to damage after the reaction liquid is frequently contacted with the miniature motor, thereby improving the use cost of the multichannel polypeptide reaction synthesizer.
For this purpose, a multichannel polypeptide reaction synthesizer is proposed.
Disclosure of Invention
The invention aims to provide a multichannel polypeptide reaction synthesizer, wherein the moving distance of a first piston can be intuitively observed through scale marks on a first piston rod, so that the adding quantity of reaction liquid can be conveniently and accurately controlled, the reaction liquid in a reaction liquid storage tank can be sucked into a syringe through a liquid suction pipe and a connecting pipe by pulling the first piston upwards, the reaction liquid can be conveniently and continuously added into the syringe without mixing air, and the problems in the prior art can be solved.
In order to achieve the above purpose, the present invention provides the following technical solutions:
the utility model provides a multichannel polypeptide reaction synthesizer, includes the synthesizer body, the passageway is installed on the top of synthesizer body, a plurality of outer tubes are installed on the top of passageway, every all be connected with the injection tube in the outer tube, every the bottom of injection tube all is equipped with the injection needle, and injection needle, outer tube, passageway and synthesizer body intercommunication each other, every all install annular connecting plate on the lateral wall on injection tube top, be connected with a plurality of hasp jointly between the lateral wall on annular connecting plate and the outer tube top, every all be equipped with first piston in the injection tube, every first piston's top all installs first piston rod, every equal distance is equipped with the scale mark on the lateral wall of first piston rod, every all be connected with reaction solution and add the subassembly on the injection tube, fixed mounting has driving motor on the roof of passageway, driving motor's output is connected with the pivot, and the pivot is located the passageway, install the drain pan on the lateral wall of pivot, the bottom of pivot is connected with the puddler, and the puddler is located the synthesizer body, evenly install a plurality of heating assemblies on the stirring leaf, the stirring instrument is connected with the stirring body in the circulation, the stirring assembly is installed to a plurality of evenly install on the stirring leaf.
Connecting the injection tube to the corresponding outer tube, extending the bottom end of the injection needle to the channel, pushing the first piston rod to drive the first piston to move so as to add the reaction liquid in the injection tube to the channel and the synthesizer body, visually observing the moving distance of the first piston rod and the first piston through the scale marks on the first piston rod, thereby being convenient for accurately controlling the adding quantity of the reaction liquid, and the reaction liquid adding component connected to the injection tube can continuously add the reaction liquid into the injection tube without mixing air, thereby improving the processing quality of the multichannel polypeptide reaction synthesizer; the driving motor is arranged at the top end of the channel, so that the driving motor can be conveniently assembled, disassembled and overhauled, and the damage to the motor caused by frequent contact of the reaction liquid with the motor can be avoided, thereby reducing the use cost of the multichannel polypeptide reaction synthesizer; the bottom ends of the driving motor and the rotating shaft are connected with a stirring rod and a stirring blade, and the driving motor drives the rotating shaft and the drain pan to rotate so as to drive the stirring rod and the stirring blade to rotate, thereby facilitating the rapid and even stirring of the solution in the synthesizer body, improving the processing rate of the multichannel polypeptide reaction synthesizer and further improving the processing quality of the multichannel polypeptide reaction synthesizer; the heating component arranged in the stirring blade can uniformly heat the solution in the synthesizer body, so that the solution is kept at a temperature suitable for the polypeptide, and the specific temperature can be set according to the type of the polypeptide, thereby further improving the processing rate of the multichannel polypeptide reaction synthesizer and improving the processing quality of the multichannel polypeptide reaction synthesizer.
Preferably, every sealing bottom plate is all installed on the lateral wall of syringe bottom, reaction solution adds the subassembly including installing fluid-discharge tube and the drawing liquid pipe in the syringe bottom, first check valve is all installed to fluid-discharge tube and drawing liquid intraductal, and the injection needle is connected in the bottom of fluid-discharge tube, the one end of drawing liquid pipe is connected with the connecting pipe, and the one end of connecting pipe extends to the outside of outer tube, the one end of connecting pipe is connected with the reaction solution storage tank.
The injection tube is connected to the top end of the outer tube and the bottom end of the injection needle extends into the channel, the first piston rod is pushed downwards to drive the first piston to move, the reaction liquid in the injection tube can be added into the channel and the synthesizer body through the liquid discharge tube and the injection needle, the reaction liquid in the reaction liquid storage tank can be sucked into the injection tube through the liquid suction tube and the connecting tube by pulling the first piston rod upwards to drive the first piston to move, the reaction liquid can be conveniently and continuously added into the injection tube without mixing air, the operation is convenient, and the processing quality of the multichannel polypeptide reaction synthesizer is improved.
Preferably, the outer tube is connected to the top wall of the channel by equal threads, each outer tube is connected to an annular mounting plate by equal threads on the inner side wall of the bottom end of the outer tube, and each annular mounting plate is provided with a medical plastic diaphragm matched with the injection needle on the inner side wall of the annular mounting plate.
The medical plastic diaphragm is adopted to seal the top end and the bottom end of the outer tube, and only the bottom end of the injection needle extends to the lower side of the outer tube and is communicated with the lower side of the outer tube after the injection tube is connected to the outer tube, so that air can be prevented from being brought into the channel and the synthesizer body when the injection tube is inserted from the top end of the outer tube, and the processing quality of the multichannel polypeptide reaction synthesizer is further improved.
Preferably, the heating element includes the electrothermal tube of evenly installing in the stirring leaf, the bull stick is installed to the synthetic appearance body internal rotation, and the bottom of pivot extends to the synthetic appearance originally internally, first gear and second gear are installed respectively to the adjacent one end of pivot and bull stick, and first gear and second gear intermeshing, third gear and fourth gear are installed respectively to the adjacent one end of bull stick and puddler, and third gear and fourth gear intermeshing, the axle center department rotation on puddler top is connected with the joint, joint and electrothermal tube electric connection, and connect to fix in the synthetic appearance originally, be connected with the connecting wire on the joint, and the one end of connecting wire extends to the outside of synthetic appearance body, the one end of connecting wire is connected with the plug.
The driving motor drives the rotating shaft to rotate so as to drive the first gear, the second gear, the rotating rod, the third gear, the fourth gear, the stirring rod and the stirring blade to rotate, so that the solution in the synthesizer body is conveniently and rapidly stirred uniformly, the processing speed of the multichannel polypeptide reaction synthesizer is improved, the processing quality of the multichannel polypeptide reaction synthesizer is also improved, the electric heating tube can be electrified after the connecting wire is electrified, the stirring blade and the electric heating tube can uniformly heat the solution while rotating, the solution is kept at the temperature suitable for polypeptides, and the processing speed of the multichannel polypeptide reaction synthesizer is further improved, and the processing quality of the multichannel polypeptide reaction synthesizer is further improved.
Preferably, the nitrogen gas circulation stirring subassembly includes two cylinders of symmetry installation on synthesizer body top, two all install the second piston in the cylinder, two the second piston rod is all installed on the top of second piston, two the equal symmetry of bottom of cylinder is installed exhaust tube and blast pipe, all install the second check valve in exhaust tube and the blast pipe, and two exhaust tubes all are located the inboard top of synthesizer body, install annular aeration board on the inside wall of synthesizer body bottom, and the bottom of two blast pipes all is connected on annular aeration board, two be connected with drive assembly between second piston rod and the driving motor, and driving motor drive pivot rotates and can drive two second piston rods and two second pistons and reciprocate.
The driving motor drives the rotating shaft to rotate so as to drive the two second piston rods and the two second pistons to move up and down, the second pistons move upwards to suck nitrogen in the synthesizer body into the cylinder body through the exhaust pipe at the top end, the second pistons move downwards so as to inject the nitrogen sucked into the cylinder body into the solution through the exhaust pipe and the annular aeration plate, and the nitrogen is introduced into the solution to bubble and move upwards, so that the solution can be further stirred and mixed, the processing rate of the multichannel polypeptide reaction synthesizer is further improved, and the processing quality of the multichannel polypeptide reaction synthesizer is improved.
Preferably, two all rotate on the roof of cylinder body and install the rotary drum, and in the bottom of rotary drum all extends to the cylinder body, drive assembly is including horizontal rotation installs two installation poles on the passageway roof, two first bevel gear is all installed at the both ends of installation pole, all install on the lateral wall of pivot and two rotary drums with first bevel gear intermeshing's second bevel gear, two the second piston rod runs through two rotary drum settings respectively, two the second piston rod is two bi-directional lead screw, two the second piston rod all with the mutual screw-thread engagement of rotary drum that corresponds, and two rotary drum internal thread opposite directions.
The pivot rotates and can drive second bevel gear, first bevel gear, installation pole and rotary drum rotation, and the rotation direction of two rotary drums is the same moreover, and two second piston rods are two-way lead screw and two the second piston rod all with the mutual screw-thread engagement of rotary drum that corresponds, consequently, two second piston rods reciprocate respectively when two rotary drums's rotation direction are the same, and another cylinder body is released the inspired nitrogen gas when one cylinder body inhales nitrogen gas to the internal atmospheric pressure of synthesizer is stable of being convenient for, has promoted the security and the stability that multichannel polypeptide reaction synthesizer used, drives two second piston rods and two second piston with the help of driving motor drive pivot and reciprocates, need not additionally to set up actuating device, thereby reduced multichannel polypeptide reaction synthesizer's manufacturing and use cost.
Compared with the prior art, the invention has the beneficial effects that:
1. the first piston is pushed downwards to add the reaction liquid in the injection tube into the channel and the synthesizer body through the liquid discharge tube and the injection needle, and the moving distance of the first piston can be visually observed through the scale marks on the first piston rod, so that the adding quantity of the reaction liquid can be conveniently and accurately controlled, the reaction liquid in the reaction liquid storage tank can be sucked into the injection tube through the liquid suction tube and the connecting tube by pulling the first piston upwards, the reaction liquid can be conveniently and continuously added into the injection tube without mixing air, the operation is convenient, and the processing quality of the multichannel polypeptide reaction synthesizer is improved.
2. The driving motor is arranged at the top end of the channel, so that the driving motor can be conveniently assembled, disassembled and overhauled, and the motor is prevented from being damaged due to frequent contact between the reaction liquid and the motor, thereby reducing the use cost of the multichannel polypeptide reaction synthesizer.
3. The bottom of driving motor and pivot is connected with puddler and stirring leaf, and driving motor drive pivot and drain pan rotate can drive puddler and stirring leaf and rotate to be convenient for evenly stirring the solution in the synthesizer body fast, not only promoted multichannel polypeptide reaction synthesizer's processing rate, also further improved multichannel polypeptide reaction synthesizer's processingquality.
4. The heating component arranged in the stirring blade can uniformly heat the solution in the synthesizer body, so that the solution is kept at a temperature suitable for the polypeptide, thereby further improving the processing rate of the multichannel polypeptide reaction synthesizer and improving the processing quality of the multichannel polypeptide reaction synthesizer.
5. The nitrogen circulation stirring assembly can continuously inject nitrogen into the synthesizer body, and the nitrogen is introduced into the solution to bubble and move upwards, so that the solution can be further stirred and mixed, the processing rate of the multichannel polypeptide reaction synthesizer is further improved, and the processing quality of the multichannel polypeptide reaction synthesizer is improved.
Drawings
FIG. 1 is a schematic side view of the present invention;
FIG. 2 is a side elevational view in cross-section of the present invention;
FIG. 3 is a top view of a stirring vane portion of the present invention;
FIG. 4 is a schematic view of the transmission assembly portion of the present invention;
fig. 5 is an enlarged view of the a-part structure of the present invention;
fig. 6 is an enlarged view of the B-section structure of the present invention.
In the figure: 1. a synthesizer body; 2. a channel; 3. an outer tube; 4. a syringe; 5. an injection needle; 6. an annular connecting plate; 7. a hasp; 8. a first piston; 9. a first piston rod; 10. scale marks; 11. sealing the bottom plate; 12. a liquid discharge pipe; 13. a liquid suction pipe; 14. a connecting pipe; 15. an annular mounting plate; 16. a driving motor; 17. a rotating shaft; 18. a drain pan; 19. a stirring rod; 20. stirring the leaves; 21. a rotating rod; 22. a first gear; 23. a second gear; 24. a third gear; 25. a fourth gear; 26. a joint; 27. a connecting wire; 28. a cylinder; 29. a second piston; 30. a second piston rod; 31. an exhaust pipe; 32. an exhaust pipe; 33. an annular aeration plate; 34. a rotating drum; 35. a mounting rod; 36. a first bevel gear; 37. and a second bevel gear.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.
Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more of the described features. In the description of the present invention, the meaning of "a plurality" is two or more, unless explicitly defined otherwise. Furthermore, the terms "mounted," "connected," "coupled," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
Referring to fig. 1 to 6, the present invention provides a multi-channel polypeptide reaction synthesizer, which has the following technical scheme:
a multi-channel polypeptide reaction synthesizer comprises a synthesizer body 1, a channel 2 is arranged at the top end of the synthesizer body 1, a plurality of outer tubes 3 are arranged at the top end of the channel 2, each outer tube 3 is internally connected with an injection tube 4, the bottom end of each injection tube 4 is provided with an injection needle 5, the injection needles 5, the outer tubes 3, the channel 2 and the synthesizer body 1 are mutually communicated, an annular connecting plate 6 is arranged on the outer side wall of the top end of each injection tube 4, a plurality of buckles 7 are jointly connected between the annular connecting plate 6 and the outer side wall of the top end of the outer tube 3, the annular connecting plate 6 and the injection tubes 4 can be conveniently and fixedly connected onto the outer tube 3 by using the buckles 7 after the injection tubes 4 are inserted into the outer tube 3, each injection tube 4 is internally provided with a first piston 8, the top end of each first piston 8 is provided with a first piston rod 9, the equal distance is equipped with scale mark 10 on the lateral wall of every first piston rod 9, all be connected with reaction liquid on every injection tube 4 and add the subassembly, fixedly mounted has driving motor 16 on the roof of passageway 2, driving motor 16's output is connected with pivot 17, and pivot 17 is located passageway 2, install drain pan 18 on the lateral wall of pivot 17, drain pan 18 rotates and can break up the reaction liquid that flows from injection needle 5 bottom, thereby the reaction liquid evenly flows to the synthesizer body 1 of being convenient for, be connected with puddler 19 with the bottom of pivot 17, and puddler 19 is located synthesizer body 1, evenly install a plurality of stirring leaf 20 on the lateral wall of puddler 19, install heating element jointly in the stirring leaf 20, be connected with nitrogen gas circulation stirring subassembly on the synthesizer body 1.
Connecting the injection tube 4 into the corresponding outer tube 3 and extending the bottom end of the injection needle 5 into the channel 2, pushing the first piston rod 9 to drive the first piston 8 to move so as to add the reaction liquid in the injection tube 4 into the channel 2 and the synthesizer body 1, visually observing the moving distance of the first piston rod 9 and the first piston 8 through the scale marks 10 on the first piston rod 9, thereby being convenient for accurately controlling the adding quantity of the reaction liquid, and the reaction liquid adding component connected on the injection tube 4 can continuously add the reaction liquid into the injection tube 4 without mixing air, thereby improving the processing quality of the multichannel polypeptide reaction synthesizer; the driving motor 16 is arranged at the top end of the channel 2, so that the driving motor 16 can be conveniently assembled, disassembled and overhauled, and the motor is prevented from being damaged due to frequent contact between the reaction liquid and the motor, thereby reducing the use cost of the multichannel polypeptide reaction synthesizer; the stirring rod 19 and the stirring blade 20 are connected to the bottom ends of the driving motor 16 and the rotating shaft 17, and the driving motor 16 drives the rotating shaft 17 and the drain pan 18 to rotate so as to drive the stirring rod 19 and the stirring blade 20 to rotate, so that the solution in the synthesizer body 1 can be stirred uniformly, the processing rate of the multichannel polypeptide reaction synthesizer is improved, and the processing quality of the multichannel polypeptide reaction synthesizer is further improved; the heating component arranged in the stirring blade 20 can uniformly heat the solution in the synthesizer body 1, so that the solution is kept at a temperature suitable for the polypeptide, and the specific temperature can be set according to the type of the polypeptide, thereby further improving the processing rate of the multichannel polypeptide reaction synthesizer and improving the processing quality of the multichannel polypeptide reaction synthesizer.
As an embodiment of the present invention, referring to fig. 1, 2 and 5, a sealing bottom plate 11 is installed on an outer sidewall of a bottom end of each injection tube 4, the sealing bottom plate 11 can seal the outer tube 3 and the injection tube 4 to a top end of the channel 2 after the injection tube 4 is inserted into the outer tube 3, the reaction solution adding assembly comprises a liquid discharge tube 12 and a liquid suction tube 13 installed at the bottom end of the injection tube 4, first check valves are installed in the liquid discharge tube 12 and the liquid suction tube 13, the injection needle 5 is connected to the bottom end of the liquid discharge tube 12, the first check valves are installed to enable the liquid discharge tube 12 to discharge liquid outwards only, the liquid suction tube 13 can absorb liquid only, one end of the liquid suction tube 13 is connected with a connecting tube 14, one end of the connecting tube 14 extends to the outer side of the outer tube 3, and one end of the connecting tube 14 is connected with a reaction solution storage tank.
The injection tube 4 is connected to the top end of the outer tube 3, the bottom end of the injection needle 5 extends into the channel 2, the first piston rod 9 is pushed downwards to drive the first piston 8 to move, the reaction liquid in the injection tube 4 can be added into the channel 2 and the synthesizer body 1 through the liquid discharge tube 12 and the injection needle 5, the reaction liquid in the reaction liquid storage tank can be sucked into the injection tube 4 through the liquid suction tube 13 and the connecting tube 14 by pulling the first piston rod 9 upwards to drive the first piston 8 to move, the reaction liquid can be conveniently and continuously added into the injection tube 4 without mixing air, the operation is convenient, and the processing quality of the multichannel polypeptide reaction synthesizer is improved.
As an embodiment of the present invention, referring to fig. 1, 2 and 5, the outer tubes 3 are all screwed on the top wall of the channel 2, the inner side wall at the bottom end of each outer tube 3 is all screwed with the annular mounting plate 15, and the inner side wall of each annular mounting plate 15 is all provided with the medical plastic membrane matched with the injection needle 5, so that the outer tube 3 and the annular mounting plate 15 can be conveniently assembled and disassembled, and the medical plastic membrane can be conveniently replaced.
The medical plastic diaphragm is adopted to seal the top end and the bottom end of the outer tube 3, and only the bottom end of the injection needle 5 extends to the lower side of the outer tube 3 and is communicated with the lower side of the outer tube 3 after the injection tube 4 is connected to the outer tube 3, so that air can be prevented from being brought into the channel 2 and the synthesizer body 1 when the injection tube 4 is inserted from the top end of the outer tube 3, and the processing quality of the multichannel polypeptide reaction synthesizer is further improved.
As an embodiment of the present invention, referring to fig. 2, 3 and 6, the heating assembly includes an electrothermal tube uniformly installed in the stirring blade 20, the rotation of the rotation shaft 21 is installed in the synthesizer body 1, the bottom end of the rotation shaft 17 extends into the synthesizer body 1, the adjacent ends of the rotation shaft 17 and the rotation shaft 21 are respectively installed with the first gear 22 and the second gear 23, the first gear 22 is meshed with the second gear 23, the adjacent ends of the rotation shaft 21 and the stirring rod 19 are respectively installed with the third gear 24 and the fourth gear 25, the third gear 24 is meshed with the fourth gear 25, the shaft center of the top end of the stirring rod 19 is rotatably connected with the joint 26, so that the rotation shaft 17 is convenient to rotate to drive the stirring rod 19 and the stirring blade 20, the joint 26 can be conveniently connected to the shaft center of the top end of the stirring rod 19, the joint 26 is electrically connected with the electrothermal tube, the joint 26 is fixed in the synthesizer body 1, the connector is arranged between the electrothermal tube and the joint 26, the electrothermal tube and the connector can rotate relatively to the synthesizer body 1, the joint 26 is connected with the joint 27, and one end of the connecting wire 27 extends to the outside of the synthesizer body 1, and the connector 27 is connected with the plug.
The driving motor 16 drives the rotating shaft 17 to rotate so as to drive the first gear 22, the second gear 23, the rotating rod 21, the third gear 24, the fourth gear 25, the stirring rod 19 and the stirring blade 20 to rotate, so that the solution in the synthesizer body 1 is conveniently and rapidly stirred uniformly, the processing speed of the multichannel polypeptide reaction synthesizer is improved, the processing quality of the multichannel polypeptide reaction synthesizer is also improved, the electric heating tube can be electrified after the connecting wire 27 is electrified, the stirring blade 20 and the electric heating tube can uniformly heat the solution while rotating, the solution is kept at a temperature suitable for polypeptides, and the processing speed of the multichannel polypeptide reaction synthesizer is further improved, and the processing quality of the multichannel polypeptide reaction synthesizer is improved.
As an embodiment of the present invention, referring to fig. 2, the nitrogen circulation stirring assembly includes two cylinders 28 symmetrically installed at the top end of the synthesizer body 1, second pistons 29 are installed in the two cylinders 28, second piston rods 30 are installed at the top ends of the two second pistons 29, exhaust pipes 31 and exhaust pipes 32 are symmetrically installed at the bottom ends of the two cylinders 28, second check valves are installed in the exhaust pipes 31 and the exhaust pipes 32, the two exhaust pipes 31 are located at the top end of the inner side of the synthesizer body 1, an annular aeration plate 33 is installed on the inner side wall of the bottom end of the synthesizer body 1, the bottom ends of the two exhaust pipes 32 are connected to the annular aeration plate 33, a transmission assembly is connected between the two second piston rods 30 and the driving motor 16, and the driving motor 16 drives the rotating shaft 17 to rotate so as to drive the two second piston rods 30 and the two second pistons 29 to move up and down.
The driving motor 16 drives the rotating shaft 17 to rotate so as to drive the two second piston rods 30 and the two second pistons 29 to move up and down, the second pistons 29 move up and can pump nitrogen in the synthesizer body 1 into the cylinder 28 through the exhaust pipe 31 at the top end, the second pistons 29 move down and can pump the nitrogen sucked into the cylinder 28 into the solution through the exhaust pipe 32 and the annular aeration plate 33, the nitrogen is introduced into the solution to bubble and move up, and therefore the solution can be further stirred and mixed, and the processing rate of the multichannel polypeptide reaction synthesizer is further improved, and the processing quality of the multichannel polypeptide reaction synthesizer is further improved.
As an embodiment of the present invention, referring to fig. 1, 2 and 4, the top walls of the two cylinders 28 are rotatably mounted with the drums 34, and the bottom ends of the drums 34 extend into the cylinders 28, the transmission assembly includes two mounting rods 35 horizontally rotatably mounted on the top wall of the channel 2, the two mounting rods 35 are mounted with first bevel gears 36 at both ends, the rotating shafts 17 and the outer side walls of the two drums 34 are mounted with second bevel gears 37 engaged with the first bevel gears 36, the two second piston rods 30 are respectively disposed through the two drums 34, the two second piston rods 30 are respectively bidirectional screw rods, the two second piston rods 30 are respectively engaged with the corresponding drums 34 in a threaded manner, and the directions of the internal threads of the two drums 34 are opposite, so that the two second piston rods 30 are opposite in moving directions when the rotating directions of the two drums 34 are identical, and the two second pistons 29 are respectively rectangular, so that the two second piston rods 30 are respectively moved up and down when the rotating directions of the two drums 34 are identical.
The rotation of the rotating shaft 17 can drive the second bevel gear 37, the first bevel gear 36, the mounting rod 35 and the rotating drums 34 to rotate, the rotation directions of the two rotating drums 34 are the same, the two second piston rods 30 are two-way screw rods and the two second piston rods 30 are in threaded engagement with the corresponding rotating drums 34, so that when the rotation directions of the two rotating drums 34 are the same, the two second piston rods 30 move up and down respectively, one cylinder 28 pushes out the sucked nitrogen when the other cylinder 28 sucks the nitrogen, thereby being convenient for stabilizing the air pressure in the synthesizer body 1, improving the use safety and stability of the multichannel polypeptide reaction synthesizer, and driving the rotating shaft 17 to drive the two second piston rods 30 and the two second piston rods 29 to move up and down by means of the driving motor 16 without additional driving equipment, thereby reducing the manufacturing and use cost of the multichannel polypeptide reaction synthesizer.
Working principle: connecting the injection tube 4 into the corresponding outer tube 3 and extending the bottom end of the injection needle 5 into the channel 2, pushing the first piston rod 9 downwards to drive the first piston 8 to move, so that the reaction liquid in the injection tube 4 can be added into the channel 2 and the synthesizer body 1 through the liquid discharge tube 12 and the injection needle 5, the moving distance of the first piston rod 9 and the first piston 8 can be intuitively observed through the scale marks 10 on the first piston rod 9, thereby being convenient for accurately controlling the adding quantity of the reaction liquid, and the reaction liquid in the reaction liquid storage tank can be conveniently and continuously added into the injection tube 4 without air mixing by pulling the first piston rod 9 upwards to drive the first piston 8 to move; the driving motor 16 drives the rotating shaft 17 to rotate and can drive the first gear 22, the second gear 23, the rotating rod 21, the third gear 24, the fourth gear 25, the stirring rod 19 and the stirring blade 20 to rotate, so that the solution in the synthesizer body 1 can be stirred uniformly, the electric heating tube can be electrified after the connecting wire 27 is electrified, the stirring blade 20 and the electric heating tube rotate and can uniformly heat the solution, the solution is kept at a temperature suitable for polypeptides, and the processing rate of the multichannel polypeptide reaction synthesizer is improved and the processing quality of the multichannel polypeptide reaction synthesizer is improved; the driving motor 16 drives the rotating shaft 17 to rotate so as to drive the second bevel gear 37, the first bevel gear 36, the mounting rod 35 and the rotating drums 34 to rotate, the rotating directions of the two rotating drums 34 are the same, the two second piston rods 30 are two-way screw rods and the two second piston rods 30 are in threaded engagement with the corresponding rotating drums 34, therefore, when the rotating directions of the two rotating drums 34 are the same, the two second piston rods 30 move up and down respectively, the second piston 29 moves up to suck nitrogen in the synthesizer body 1 into the cylinder body 28 through the exhaust pipe 31 at the top end, the second piston 29 moves down to inject the nitrogen sucked into the cylinder body 28 into the solution through the exhaust pipe 32 and the annular aeration plate 33, and when one cylinder body 28 sucks the nitrogen, the other cylinder body 28 pushes out the sucked nitrogen, so that the air pressure in the synthesizer body 1 is stable, the nitrogen is blown into the solution and moves up, and the solution can be further stirred and mixed.
The electric elements are all connected with an external main controller and 220V mains supply through a transformer, and the main controller can be conventional known equipment for controlling a computer and the like.
Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (4)
1. The utility model provides a multichannel polypeptide reaction synthesizer, includes synthesizer body (1), its characterized in that: the utility model discloses a synthesizer, including synthesizer body (1), outer tube (3), first piston (9) are all installed on the top of channel (2), every all be connected with injection tube (4) in outer tube (3), every injection tube (4)'s bottom all is equipped with injection needle (5), and injection needle (5), outer tube (3), channel (2) and synthesizer body (1) communicate each other, every all install annular connecting plate (6) on the lateral wall on injection tube (4) top, be connected with a plurality of hasp (7) jointly between the lateral wall on annular connecting plate (6) and outer tube (3) top, every all be equipped with first piston (8) in injection tube (4), every equal distance in the lateral wall of first piston (9) is equipped with scale mark (10), every all be connected with reaction liquid and add the subassembly on injection tube (4), motor (16) fixed mounting have on the lateral wall on channel (2) top wall (16) on the motor, drive shaft (17) are connected with in the top wall (17) and are connected with pivot (17) in the pivot (17) are connected with in the pivot (17), the stirring rod (19) is positioned in the synthesizer body (1), a plurality of stirring blades (20) are uniformly arranged on the outer side wall of the stirring rod (19), heating components are commonly arranged in the stirring blades (20), and the synthesizer body (1) is connected with a nitrogen circulation stirring component;
the heating assembly comprises an electric heating tube uniformly arranged in a stirring blade (20), a rotating rod (21) is rotatably arranged in a synthesizer body (1), the bottom end of a rotating shaft (17) extends into the synthesizer body (1), a first gear (22) and a second gear (23) are respectively arranged at adjacent ends of the rotating shaft (17) and the rotating rod (21), the first gear (22) and the second gear (23) are meshed with each other, a third gear (24) and a fourth gear (25) are respectively arranged at adjacent ends of the rotating rod (21) and the stirring rod (19), the third gear (24) and the fourth gear (25) are meshed with each other, a joint (26) is rotationally connected at the axis of the top end of the stirring rod (19), the joint (26) is electrically connected with the electric heating tube, the joint (26) is fixed in the synthesizer body (1), a connecting wire (27) is connected to the joint (26), one end of the connecting wire (27) extends to the outer side of the synthesizer body (1), and one end of the connecting wire (27) is connected with a plug;
the nitrogen gas circulation stirring subassembly is including two cylinder bodies (28) of symmetry installation at synthesizer body (1) top, two all install second piston (29) in cylinder body (28), two second piston (29) are all installed on the top of second piston (29), two exhaust tube (31) and blast pipe (32) are all installed to the bottom of cylinder body (28) symmetry, all install second check valve in exhaust tube (31) and blast pipe (32), and two exhaust tube (31) all are located the inboard top of synthesizer body (1), install annular aeration board (33) on the inside wall of synthesizer body (1) bottom, and the bottom of two blast pipe (32) all is connected on annular aeration board (33), two be connected with drive assembly between second piston rod (30) and driving motor (16), and driving motor (16) drive pivot (17) rotate and can drive two second piston rods (30) and two second piston (29) reciprocates.
2. The multi-channel polypeptide reaction synthesizer according to claim 1, wherein: every sealing bottom plate (11) are all installed on the lateral wall of injection tube (4) bottom, reaction solution adds the fluid-discharge tube (12) and the drawing liquid pipe (13) of subassembly in injection tube (4) bottom including installing, all install first check valve in fluid-discharge tube (12) and drawing liquid pipe (13), and injection needle (5) connect the bottom in fluid-discharge tube (12), the one end of drawing liquid pipe (13) is connected with connecting pipe (14), and the one end of connecting pipe (14) extends to the outside of outer tube (3), the one end of connecting pipe (14) is connected with the reaction solution storage tank.
3. The multi-channel polypeptide reaction synthesizer according to claim 1, wherein: the outer tube (3) is connected to the top wall of the channel (2) in a threaded mode, an annular mounting plate (15) is connected to the inner side wall of the bottom end of each outer tube (3) in a threaded mode, and medical plastic diaphragms matched with the injection needles (5) are arranged on the inner side wall of each annular mounting plate (15).
4. The multi-channel polypeptide reaction synthesizer according to claim 1, wherein: the rotary cylinder is characterized in that the rotary cylinders (34) are rotatably arranged on the top wall of the cylinder body (28), the bottoms of the rotary cylinders (34) are respectively extended into the cylinder body (28), the transmission assembly comprises two mounting rods (35) horizontally rotatably arranged on the top wall of the channel (2), first bevel gears (36) are respectively arranged at two ends of the mounting rods (35), second bevel gears (37) meshed with the first bevel gears (36) are respectively arranged on the outer side walls of the rotary shafts (17) and the two rotary cylinders (34), the two second piston rods (30) respectively penetrate through the two rotary cylinders (34), the two second piston rods (30) are respectively bidirectional screw rods, the two second piston rods (30) are respectively meshed with the corresponding rotary cylinders (34) in a threaded manner, and the directions of the internal threads of the two rotary cylinders (34) are opposite.
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| JPS58148896A (en) * | 1982-02-26 | 1983-09-05 | Shimadzu Corp | Automated microsynthesizer for dna or the like |
| CA1266181A (en) * | 1984-05-16 | 1990-02-27 | Frank A. Walton | Apparatus for injecting measured quantities of liquid into a fluid stream |
| FR2582655B1 (en) * | 1985-06-03 | 1988-12-23 | Centre Nat Rech Scient | SOLID PHASE SEMI-AUTOMATIC PEPTIDE MULTI-SYNTHESIZER |
| CN206435112U (en) * | 2017-01-29 | 2017-08-25 | 孔令廷 | A kind of medicament mixing device |
| CN106892959B (en) * | 2017-03-08 | 2021-01-12 | 河南工程学院 | Multi-channel polypeptide solid phase synthesis method |
| CN108579518A (en) * | 2018-01-19 | 2018-09-28 | 东莞嘉蓝素环保科技有限公司 | A kind of urea for vehicle processing quantitative proportioning homogeneous device |
| CN108623651A (en) * | 2018-06-19 | 2018-10-09 | 南京肽业生物科技有限公司 | Efficient Peptide systhesis device |
| CN208883754U (en) * | 2018-07-24 | 2019-05-21 | 南京杰肽生物科技有限公司 | A kind of semi-automatic multi-channel solid phase peptide synthesizer |
| CN213724125U (en) * | 2020-05-31 | 2021-07-20 | 嘉兴市第二医院 | Quantitative injection device for anesthesia operation |
| CN212882430U (en) * | 2020-07-14 | 2021-04-06 | 湖北强耀生物科技有限公司 | Polypeptide synthesis reaction device |
| CN213609001U (en) * | 2020-11-09 | 2021-07-06 | 胡姣兰 | Poultry syringe for livestock raising and beast medicine |
| CN112619583A (en) * | 2021-01-08 | 2021-04-09 | 黑龙江思莱博科技股份有限公司 | Solid phase polypeptide synthesizer |
| CN113274961A (en) * | 2021-05-31 | 2021-08-20 | 浙江宝泽高分子材料有限公司 | Preparation method and production equipment of organic silicon foam homogenizing agent |
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