CN112619583A - Solid phase polypeptide synthesizer - Google Patents
Solid phase polypeptide synthesizer Download PDFInfo
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- CN112619583A CN112619583A CN202110021212.8A CN202110021212A CN112619583A CN 112619583 A CN112619583 A CN 112619583A CN 202110021212 A CN202110021212 A CN 202110021212A CN 112619583 A CN112619583 A CN 112619583A
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- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0046—Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
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- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0006—Controlling or regulating processes
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- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0006—Controlling or regulating processes
- B01J19/0013—Controlling the temperature of the process
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- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/0053—Details of the reactor
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- B01J19/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J19/18—Stationary reactors having moving elements inside
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- B01J4/00—Feed or outlet devices; Feed or outlet control devices
- B01J4/001—Feed or outlet devices as such, e.g. feeding tubes
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- B01J2204/00—Aspects relating to feed or outlet devices; Regulating devices for feed or outlet devices
- B01J2204/002—Aspects relating to feed or outlet devices; Regulating devices for feed or outlet devices the feeding side being of particular interest
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00049—Controlling or regulating processes
- B01J2219/00051—Controlling the temperature
- B01J2219/00054—Controlling or regulating the heat exchange system
- B01J2219/00056—Controlling or regulating the heat exchange system involving measured parameters
- B01J2219/00058—Temperature measurement
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- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00049—Controlling or regulating processes
- B01J2219/00051—Controlling the temperature
- B01J2219/00074—Controlling the temperature by indirect heating or cooling employing heat exchange fluids
- B01J2219/00076—Controlling the temperature by indirect heating or cooling employing heat exchange fluids with heat exchange elements inside the reactor
- B01J2219/00085—Plates; Jackets; Cylinders
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- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
- B01J2219/00049—Controlling or regulating processes
- B01J2219/00051—Controlling the temperature
- B01J2219/00074—Controlling the temperature by indirect heating or cooling employing heat exchange fluids
- B01J2219/00087—Controlling the temperature by indirect heating or cooling employing heat exchange fluids with heat exchange elements outside the reactor
- B01J2219/00092—Tubes
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- B01J2219/00—Chemical, physical or physico-chemical processes in general; Their relevant apparatus
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- B01J2219/00162—Controlling or regulating processes controlling the pressure
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Abstract
The invention discloses a solid-phase polypeptide synthesis device, which comprises a heat transfer box body, wherein a synthesis barrel is fixedly connected to the inner wall of the heat transfer box body, a servo motor is fixedly connected to the center of the outer top surface of the synthesis barrel through a screw, the output end of the servo motor penetrates through the top surface of the synthesis barrel and is fixedly connected with a stirring paddle, a heating sheet is fixedly connected to the inner bottom surface of the synthesis barrel, a heat transfer plate is fixedly connected to the inner wall of the lower end of the synthesis barrel, a discharge pipe is further connected to the center of the bottom surface of the synthesis barrel in a penetrating manner, an electromagnetic valve is fixedly arranged on the inner wall of the discharge pipe, a material injection mechanism is further arranged on one side of the. The device has reasonable design and stable operation, the adhesive is added in a slow injection mode, the phenomenon that reactants adhere to the barrel wall is well avoided, and the device is convenient to clean, so that a certain guarantee is provided for high-quality and high-efficiency preparation of solid-phase polypeptide.
Description
Technical Field
The invention relates to the technical field of solid-phase polypeptide production equipment, in particular to a solid-phase polypeptide synthesis device.
Background
The polypeptide is a compound formed by connecting alpha-amino acids together by peptide bonds, is an intermediate product of protein hydrolysis, is a compound formed by dehydrating and condensing two amino acid molecules and is called dipeptide, and also includes tripeptide, tetrapeptide, pentapeptide and the like in the same way, and is a compound formed by dehydrating and condensing 10-100 amino acid molecules and is called polypeptide, and the synthesis device is used.
Through retrieval, the patent of application No. 201921912922.4 discloses a solid-phase polypeptide synthesis device, which comprises a polypeptide synthesis cylinder with an opening at the upper side and a cylinder cover with a sealing cover arranged at the upper end of the polypeptide synthesis cylinder, the lower end of the cylinder cover is fixedly connected with a down-link rod at an eccentric position, the outer wall of the down-link rod is fixedly connected with two parallel intercepting nets, the edges of the intercepting nets are fixedly connected with a coaming plate, the outer peripheral wall of the coaming plate is in contact with the inner peripheral wall of the polypeptide synthesis cylinder in an attaching manner, the middle part of the upper end of the cylinder cover is connected with a feed pipe, the upper end of the feed pipe is connected with a feed hopper, the feed pipe is provided with a feed valve, the outer wall of the lower end of the polypeptide synthesis cylinder is communicated with a nitrogen inlet pipe, one end of the nitrogen inlet pipe, which is far away from the polypeptide synthesis cylinder, is connected with a nitrogen tank, the backflow pipe is provided with a backflow valve, the nitrogen inlet pipe between the air pump and the polypeptide synthesis barrel is communicated with the pressure filtration pipe, one end, far away from the nitrogen inlet pipe, of the pressure filtration pipe is communicated with the outer wall of the upper end of the polypeptide synthesis barrel, the inner bottom wall of the polypeptide synthesis barrel is communicated with the discharge pipe, and the discharge pipe is provided with a discharge valve.
The device can not thoroughly scrape the reactants attached to the barrel wall during synthesis, and the device is inconvenient to clean after reaction, so that the labor cost of workers is greatly increased, and the working requirement of high efficiency and energy conservation is difficult to meet, so that the research on the solid-phase polypeptide synthesis device is necessary.
Disclosure of Invention
The invention aims to solve the problems of labor consumption and low efficiency in the prior art, and provides a solid-phase polypeptide synthesis device.
In order to achieve the purpose, the invention adopts the following technical scheme:
a solid phase polypeptide synthesis device comprises a heat transfer box body, wherein a synthesis barrel is fixedly connected to the inner wall of the heat transfer box body, a servo motor is fixedly connected to the center of the outer top surface of the synthesis barrel through a screw, the output end of the servo motor penetrates through the top surface of the synthesis barrel, a stirring paddle is fixedly connected to the top surface of the synthesis barrel, a heating plate is fixedly connected to the inner wall of the lower end of the synthesis barrel, a heat transfer plate is fixedly connected to the inner wall of the lower end of the synthesis barrel, a discharging pipe is further penetratingly connected to the center of the bottom surface of the synthesis barrel, a solenoid valve is fixedly mounted on the inner wall of the discharging pipe, a material injection mechanism is further mounted on one side of the top surface of the synthesis barrel and comprises a discharging barrel and an air pump, the discharging barrel is penetratingly connected to the top surface of the synthesis barrel through a screw, an air guide pipe is connected between the output, the feeding device is characterized in that one side of the blanking barrel is further connected with a feeding pipe in a through mode, a check valve is installed on the inner wall of the feeding pipe, a feeding funnel is fixedly connected to the top end of the feeding pipe, the middle of the air duct is further connected with an air release pipe in a through mode, a first control valve is further installed on the air release pipe, a water tank is arranged on one side of the heat transfer box body, a heating pipe is fixed on the inner bottom surface of the water tank, a water inlet pipe is further connected between one side of the lower end of the heat transfer box body and the water tank in a through mode, a second control valve and a first water pump are installed on the water inlet pipe, a water return pipe is further connected between one side of the top surface of the water tank body and the upper end of the heat transfer box body in a through mode, a cooling pipe is further fixed on the inner wall, temperature sensor and pressure sensor are installed to synthetic bucket top surface one side, and synthetic bucket deviates from heat transfer box one end and still rotates and be connected with control panel, heat transfer box rear side still is provided with stores up the nitrogen bucket, stores up and has had the gas tube between nitrogen bucket and the synthetic bucket through connection, installs air pump two on the gas tube.
Preferably, the inner wall of the upper end of the synthesis barrel is also fixedly connected with a hollow ring, the bottom surface of the hollow ring is provided with a water spray nozzle, a water guide pipe is further connected between the hollow ring and the water inlet pipe in a through manner, and a third control valve is arranged on the water guide pipe.
Preferably, the water mist spray head is provided with a plurality of water mist spray heads which are uniformly distributed on the inner bottom surface of the hollow ring.
Preferably, the supporting plates are fixedly connected between the lower ends of the water tank and the storage tank body and the side wall of the heat transfer tank body, and the first water pump and the second water pump are fixedly connected to the top surfaces of the supporting plates through screws.
Preferably, the heating plate is annularly arranged, the upper end of the discharge pipe penetrates through the heat transfer plate, and sealing gaskets are fixedly connected at the joint of the discharge pipe, the heat transfer box body and the synthesis barrel.
Preferably, the inner bottom surface of the synthesis barrel is provided with a conical groove, the extrusion block is arranged in a conical shape, and the shape of the extrusion block is matched with the conical groove.
Preferably, the radiating pipe is arranged in an S shape, and the radiating pipe is sleeved at the outer side of the synthesis barrel.
Preferably, the elastic ropes are provided with a plurality of elastic ropes and are uniformly distributed on the top surface of the extrusion block.
Preferably, the output ends of the temperature sensor and the pressure sensor are electrically connected with the input end of the control panel.
Compared with the prior art, the invention provides a solid-phase polypeptide synthesis device, which has the following beneficial effects:
1. the device has reasonable design and stable operation, the adhesive is added in a slow injection mode, the phenomenon that reactants adhere to the barrel wall is well avoided, and the device is convenient and fast to clean, so that a certain guarantee is provided for high-quality and high-efficiency preparation of solid-phase polypeptide;
2. according to the invention, the blanking barrel is connected to one side of the top surface of the synthesis barrel in a penetrating manner, the binder is placed in the blanking barrel after liquid injection is completed, then the air is pumped into the blanking barrel by the air pump, and the extrusion block continuously moves downwards under the action of air pressure to continuously extrude the binder out of the blanking barrel, so that the phenomenon that a large amount of binder is simultaneously thrown into the blanking barrel to adhere to the wall of the barrel is well avoided, the mixing is uniform, and a certain guarantee is provided for high-quality synthesis of solid-phase polypeptide;
3. when the device is used, the heat transfer box body is additionally arranged on the outer side of the synthesis barrel, when solid-phase polypeptide is synthesized, water is heated through the heating pipe, then hot water is injected into the heat transfer box body through the first water pump, the hot water plays a role in heat preservation on the synthesis barrel during solid-phase polypeptide synthesis, so that stable synthesis of the solid-phase polypeptide is guaranteed, after the solid-phase polypeptide is completed, cooling liquid is pumped into the heat dissipation pipe through the second water pump to realize rapid condensation on the synthesis barrel, the device meets the requirements of heat preservation and rapid heat dissipation, and a certain guarantee is provided for high-quality synthesis of the solid-phase polypeptide;
4. according to the invention, while solid-phase polypeptide is synthesized, the temperature sensor and the pressure sensor monitor the temperature and the pressure in the synthesis barrel in real time, and monitoring information is displayed on the control panel, so that certain guarantee is provided for the high quality of the solid-phase polypeptide;
5. according to the invention, the hollow ring is fixedly connected to the inner wall of the upper end of the synthesis barrel, after the solid-phase polypeptide is discharged, the control valve II is closed and the control valve III is opened, and hot water is pumped into the hollow ring and sprayed out from the water mist spray head under the action of the water pump I, so that the self-cleaning operation of the device is completed, and a certain guarantee is provided for the precision of the multiple synthesis of the solid-phase polypeptide.
Drawings
FIG. 1 is a schematic structural diagram of a solid-phase polypeptide synthesis apparatus according to the present invention;
FIG. 2 is a front sectional view of a solid-phase polypeptide synthesis apparatus according to the present invention;
FIG. 3 is a top view of a solid-phase polypeptide synthesis apparatus according to the present invention;
FIG. 4 is a front sectional view of a feeding mechanism of a solid-phase polypeptide synthesis apparatus according to the present invention;
FIG. 5 is a schematic diagram of the installation of a water mist nozzle on a hollow ring in the solid phase polypeptide synthesis apparatus according to the present invention.
In the figure: the device comprises a heat transfer box body 1, a synthesis barrel 2, a servo motor 3, a stirring paddle 4, a heating sheet 5, a heat transfer plate 6, a discharge pipe 7, an electromagnetic valve 8, a material injection mechanism 9, a water tank 10, a heating pipe 11, a water inlet pipe 12, a control valve II 13, a water pump I14, a water return pipe 15, a cooling liquid storage box body 16, a heat dissipation pipe 17, a liquid inlet pipe 18, a water pump II 19, a liquid return pipe 20, a temperature sensor 21, a pressure sensor 22, a control panel 23, a nitrogen storage barrel 24, an inflation pipe 25, a gas pump II 26, a hollow ring 27, a water mist spray head 28, a water guide pipe 29, a control valve III 30, a support plate 31, a blanking barrel 91, an air pump 92, an air guide pipe 93, an extrusion block 94, an elastic rope 95, a feeding pipe 96.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments.
In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention.
Example one
Referring to fig. 1-5, a solid phase polypeptide synthesis device comprises a heat transfer box body 1, a synthesis barrel 2 is fixedly connected to the inner wall of the heat transfer box body 1, a servo motor 3 is fixedly connected to the center of the outer top surface of the synthesis barrel 2 through a screw, the output end of the servo motor 3 penetrates through the top surface of the synthesis barrel 2 and is fixedly connected with a stirring paddle 4, a heating plate 5 is fixedly connected to the inner bottom surface of the synthesis barrel 2, a heat transfer plate 6 is further fixedly connected to the inner wall of the lower end of the synthesis barrel 2, a discharge pipe 7 is further connected to the center of the bottom surface of the synthesis barrel 2 in a penetrating manner, an electromagnetic valve 8 is fixedly mounted on the inner wall of the discharge pipe 7, a material injection mechanism 9 is further mounted on one side of the top surface of the synthesis barrel 2, the material injection mechanism 9 comprises a blanking barrel 91 and an air pump 92, the blanking barrel 91, the inner wall of the blanking barrel 91 is further connected with an extruding block 94 in a sliding contact manner, elastic ropes 95 are fixed between the top surface of the extruding block 94 and the inner top surface of the blanking barrel 91, one side of the blanking barrel 91 is further connected with a feeding pipe 96 in a through manner, a through-stop valve is installed on the inner wall of the feeding pipe 96, the top end of the feeding pipe 96 is further fixedly connected with a feeding funnel 97, the middle part of the air duct 93 is further connected with an air release pipe 98 in a through manner, a control valve I99 is further installed on the air release pipe 98, a water tank 10 is arranged on one side of the heat transfer box body 1, a heating pipe 11 is fixed on the inner bottom surface of the water tank 10, a water inlet pipe 12 is further connected between one side of the lower end of the heat transfer box body 1 and the water tank 10 in a through manner, a control valve II 13 and a water pump I14 are installed on the water, it has feed liquor pipe 18 still to link up between 16 lower extremes of coolant liquid storage box and the cooling tube 17 input, install two 19 water pumps on the cooling tube 17, it has liquid return pipe 20 to link up between 16 top surfaces of coolant liquid storage box and the cooling tube 17 output, temperature sensor 21 and pressure sensor 22 are installed to 2 top surfaces of synthetic bucket one side, synthetic bucket 2 deviates from 1 one end of heat transfer box and still rotates and is connected with control panel 23, 1 rear side of heat transfer box still is provided with nitrogen storage bucket 24, it has gas tube 25 to link up between nitrogen storage bucket 24 and the synthetic bucket 2, install two 26 air pumps on the gas tube 25.
When the device is used, firstly, reactant liquid is injected into a synthesis barrel 2, then, a sticky substance is put into a blanking barrel 91, a heating sheet 5 is opened to enable the temperature in the synthesis barrel 2 to reach a certain value, at the moment, an air pump 92 pumps air into the blanking barrel 91, at the moment, an extrusion block 94 continuously moves downwards under the action of air pressure to continuously extrude a binding material out of the blanking barrel 91, then a servo motor 3 drives a stirring paddle 4 to rotate to realize solid-phase polypeptide synthesis reaction, when the device is used, a heat transfer box body 1 is additionally arranged on the outer side of the synthesis barrel 2, when solid-phase polypeptide is synthesized, water is heated through a heating pipe 11, then hot water is injected into the heat transfer box body 1 through a first water pump 14, the hot water plays a heat preservation role in the synthesis of the solid-phase polypeptide in the synthesis of the synthesis barrel 2, so as to ensure the stable synthesis of the solid-phase polypeptide, after the solid-phase polypeptide is synthesized, the condensed reactant is led out from the discharging pipe 7, thereby completing the synthesis operation of the solid-phase polypeptide.
Example two
As shown in fig. 2 and 5, in this embodiment, basically the same as that of embodiment 1, preferably, a hollow ring 27 is further fixedly connected to an inner wall of the upper end of the synthesis barrel 2, a water mist spray nozzle 28 is mounted on a bottom surface of the hollow ring 27, a water guide pipe 29 is further connected between the hollow ring 27 and the water inlet pipe 12 in a penetrating manner, and a third control valve 30 is mounted on the water guide pipe 29.
The water mist spray head 28 is provided in plurality and evenly distributed on the inner bottom surface of the hollow ring 27.
In this embodiment, the hollow ring 27 is fixed on the inner wall of the upper end of the synthesis barrel 2, after the solid-phase polypeptide is discharged, the control valve II 13 is closed and the control valve III 30 is opened, and the hot water is pumped into the hollow ring 27 and sprayed out from the water mist spray head 28 under the action of the water pump I14, so that the self-cleaning operation of the device is completed, and a certain guarantee is provided for the precision of the multiple synthesis of the solid-phase polypeptide.
EXAMPLE III
As shown in fig. 1 and 2, in this embodiment, basically the same as embodiment 1, preferably, a support plate 31 is fixedly connected between the lower ends of the water tank 10 and the storage tank 16 and the side wall of the heat transfer tank 1, and a first water pump 14 and a second water pump 19 are fixedly connected to the top surface of the support plate 31 by screws.
In this embodiment, the first water pump 14 and the second water pump 19 are both fixed on the top surface of the supporting plate 31 through screws, so that the first water pump 14 and the second water pump 19 work more stably.
Example four
As shown in fig. 2, in this embodiment, basically the same as embodiment 1, preferably, the heating sheet 5 is disposed in a ring shape, the upper end of the discharging pipe 7 penetrates through the heat transfer plate 6, and sealing gaskets are fixed at the junctions of the discharging pipe 7, the heat transfer box 1 and the synthesis barrel 2.
In this embodiment, the discharging pipe 7 is all the rigid coupling to have sealed the pad with the equal rigid coupling of heat transfer box 1 and synthetic bucket 2 handing-over department to guaranteed the device leakproofness, provide certain guarantee for solid phase polypeptide synthesis quality.
EXAMPLE five
As shown in fig. 4, this embodiment is substantially the same as embodiment 1, and preferably, the inner bottom surface of the synthesis barrel 2 is provided with a tapered groove, and the extrusion block 94 is arranged in a tapered shape, and the shape of the extrusion block 94 is matched with the tapered groove.
In this embodiment, the extruding block 94 is arranged in a cone shape, so that the sticky substance can be completely led out, and the feeding quality of the sticky substance is well ensured.
EXAMPLE six
As shown in fig. 2, this embodiment is substantially the same as embodiment 1, and preferably, the heat dissipation pipe 17 is disposed in an "S" shape, and the heat dissipation pipe 17 is sleeved at an outer position of the synthesis barrel 2.
The elastic cords 95 are provided in a plurality and uniformly distributed over the top surface of the extrusion block 94.
In this embodiment, the cooling tube 17 is "S" form setting to realize the even heat dissipation in the 2 outsides of synthesis bucket, provide certain guarantee for the quick heat dissipation of solid phase polypeptide thing.
EXAMPLE seven
As shown in fig. 1 and 2, the present embodiment is substantially the same as embodiment 1, and preferably, the output terminals of the temperature sensor 21 and the pressure sensor 22 are electrically connected to the input terminal of the control panel 23.
In this embodiment, the output terminals of the temperature sensor 21 and the pressure sensor 22 are electrically connected to the input terminal of the control panel 23, so that the device can display the temperature and the pressure in the synthesis barrel 2 in real time, thereby providing a certain guarantee for the synthesis quality of the solid-phase polypeptide.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be able to cover the technical scope of the present invention and the equivalent alternatives or modifications according to the technical solution and the inventive concept of the present invention within the technical scope of the present invention.
Claims (9)
1. The solid-phase polypeptide synthesis device comprises a heat transfer box body (1), wherein a synthesis barrel (2) is fixedly connected to the inner wall of the heat transfer box body (1), and is characterized in that a servo motor (3) is fixedly connected to the center of the outer top surface of the synthesis barrel (2) through a screw, the output end of the servo motor (3) penetrates through the top surface of the synthesis barrel (2) and is fixedly connected with a stirring paddle (4), a heating sheet (5) is fixedly connected to the inner bottom surface of the synthesis barrel (2), a heat transfer plate (6) is further fixedly connected to the inner wall of the lower end of the synthesis barrel (2), a discharge pipe (7) is further penetratingly connected to the center of the bottom surface of the synthesis barrel (2), a solenoid valve (8) is fixedly mounted on the inner wall of the discharge pipe;
the material injection mechanism (9) comprises a blanking barrel (91) and an air pump (92), the blanking barrel (91) is connected on the top surface of the synthesis barrel (2) in a penetrating way, and the air pump (92) is fixedly connected on the top surface of the synthesis barrel (2) through a screw, an air duct (93) is connected between the output end of the air pump (92) and the outer top surface of the blanking barrel (91) in a run-through manner, the inner wall of the blanking barrel (91) is also connected with an extrusion block (94) in a sliding contact manner, elastic ropes (95) are fixed between the top surface of the extrusion block (94) and the inner top surface of the blanking barrel (91), one side of the blanking barrel (91) is also connected with a feeding pipe (96) in a run-through way, the inner wall of the feeding pipe (96) is provided with a stop valve, a feeding funnel (97) is fixedly connected to the top end of the feeding pipe (96), an air release pipe (98) is connected to the middle of the air guide pipe (93) in a penetrating manner, and a first control valve (99) is further mounted on the air release pipe (98);
the water cooling device is characterized in that a water tank (10) is arranged on one side of the heat transfer box body (1), a heating pipe (11) is fixed on the inner bottom surface of the water tank (10), a water inlet pipe (12) is further connected between one side of the lower end of the heat transfer box body (1) and the water tank (10) in a penetrating manner, a control valve II (13) and a water pump I (14) are installed on the water inlet pipe (12), a water return pipe (15) is further connected between one side of the top surface of the water tank (10) and the upper end of the heat transfer box body (1) in a penetrating manner, a cooling liquid storage box body (16) is further arranged on the other side of the heat transfer box body (1), a radiating pipe (17) is further fixed on the inner wall of the heat transfer box body (1), a liquid inlet pipe (18) is further connected between the lower end of the cooling liquid storage box body (16), temperature sensor (21) and pressure sensor (22) are installed to synthetic bucket (2) top surface one side, and synthetic bucket (2) deviate from heat transfer box (1) one end and still rotate and be connected with control panel (23), heat transfer box (1) rear side still is provided with nitrogen storage bucket (24), and the through connection has gas tube (25) between nitrogen storage bucket (24) and synthetic bucket (2), installs air pump two (26) on gas tube (25).
2. The solid-phase polypeptide synthesis device according to claim 1, wherein the inner wall of the upper end of the synthesis barrel (2) is fixedly connected with a hollow ring (27), the bottom surface of the hollow ring (27) is provided with a water mist spray nozzle (28), a water guide pipe (29) is further connected between the hollow ring (27) and the water inlet pipe (12) in a penetrating manner, and a control valve III (30) is arranged on the water guide pipe (29).
3. The apparatus for solid-phase peptide synthesis according to claim 2, wherein the water spray nozzles (28) are provided in a plurality and uniformly distributed on the inner bottom surface of the hollow ring (27).
4. The solid-phase polypeptide synthesis device according to claim 1, wherein a support plate (31) is fixedly connected between the lower ends of the water tank (10) and the storage tank (16) and the side wall of the heat transfer tank (1), and the first water pump (14) and the second water pump (19) are fixedly connected to the top surface of the support plate (31) through screws.
5. The solid-phase polypeptide synthesis apparatus according to claim 1, wherein the heating plate (5) is disposed annularly, the upper end of the discharge tube (7) penetrates through the heat transfer plate (6), and the joint of the discharge tube (7) and the heat transfer box (1) and the synthesis barrel (2) is fixedly connected with a gasket.
6. The solid-phase polypeptide synthesis device according to claim 1, wherein the inner bottom surface of the synthesis barrel (2) is provided with a conical groove, the extrusion block (94) is arranged in a conical shape, and the shape of the extrusion block (94) is matched with the conical groove.
7. The apparatus for synthesizing solid phase polypeptide according to claim 1, wherein the heat dissipation pipe (17) is disposed in an "S" shape, and the heat dissipation pipe (17) is disposed at an outer position of the synthesis barrel (2).
8. The apparatus for solid phase peptide synthesis according to claim 1, wherein said elastic string (95) is provided in a plurality and uniformly distributed on the top surface of the extruded block (94).
9. The apparatus for solid phase peptide synthesis according to claim 1, wherein the output terminals of the temperature sensor (21) and the pressure sensor (22) are electrically connected to the input terminal of the control panel (23).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110021212.8A CN112619583A (en) | 2021-01-08 | 2021-01-08 | Solid phase polypeptide synthesizer |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113813903A (en) * | 2021-09-14 | 2021-12-21 | 韩青芬 | Multichannel polypeptide reaction synthesizer |
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