CN215799630U - Bioreactor - Google Patents
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- CN215799630U CN215799630U CN202122089823.4U CN202122089823U CN215799630U CN 215799630 U CN215799630 U CN 215799630U CN 202122089823 U CN202122089823 U CN 202122089823U CN 215799630 U CN215799630 U CN 215799630U
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Abstract
The present invention provides a bioreactor comprising: the culture tank is provided with an air outlet and an air inlet; the air exhaust assembly is communicated with the air outlet of the culture tank and is configured to be capable of exhausting air in the culture tank; and one end of the gas heating component is communicated with the air exhaust component, the air exhaust component can convey gas in the culture tank to the gas heating component, and the other end of the gas heating component is communicated with the air inlet of the culture tank. The air exhaust assembly sucks air in the culture tank from the air outlet of the culture tank, conveys the air in the culture tank to the gas heating assembly, and conveys the gas with the specific temperature set by a user into the culture tank through the air inlet of the culture tank by the gas heating assembly, so that oxygen and other gases on the top of the culture tank can be fully dissolved in the culture solution at the bottom of the culture tank to promote the absorption of cells. Moreover, the gas heating component can heat the air to a proper temperature and then send the air into the culture tank, and the liquid in the culture tank is also short in preheating time.
Description
Technical Field
The invention relates to the technical field of biological reaction equipment, in particular to a bioreactor.
Background
At present, the existing bioreactor usually adopts a stirrer to culture cells, and the solution and the cells are mixed by the stirring paddle, however, the cells can be damaged by the too fast rotating speed of the stirring paddle, and the cells cannot survive because the culture solution and the cells cannot be fully mixed. In addition, the existing bioreactor adopts a heating jacket to heat the culture tank, so that the operation is complicated, the temperature rise is slow, and the temperature control precision is low.
Disclosure of Invention
The present invention is directed to solving at least one of the problems of the prior art.
One aspect of the present invention provides a bioreactor.
In view of this, according to one aspect of the present invention, there is provided a bioreactor comprising: the culture tank is provided with an air outlet and an air inlet; the air exhaust assembly is communicated with the air outlet of the culture tank and is configured to be capable of exhausting air in the culture tank; and one end of the gas heating component is communicated with the air exhaust component, the air exhaust component can convey gas in the culture tank to the gas heating component, and the other end of the gas heating component is communicated with the air inlet of the culture tank.
The bioreactor provided by one embodiment of the invention comprises a culture tank, an air exhaust assembly and a gas heating assembly, wherein the culture tank is provided with an air outlet and an air inlet, and the air exhaust assembly is communicated with the air outlet of the culture tank, so that the air exhaust assembly can exhaust gas in the culture tank; one end of the gas heating component is communicated with the air exhaust component, the air exhaust component can convey gas in the culture tank to the gas heating component, and the other end of the gas heating component is communicated with the air inlet of the culture tank. The air exhaust assembly sucks air in the culture tank from the air outlet of the culture tank, conveys the air in the culture tank to the gas heating assembly, and conveys the gas with the specific temperature set by a user into the culture tank through the air inlet of the culture tank by the gas heating assembly, so that oxygen and other gases on the top of the culture tank can be fully dissolved in the culture solution at the bottom of the culture tank to promote the absorption of cells. And, gaseous heating element can send into the culture tank after the suitable temperature of air heating again, compares in the technical scheme that twines the heater strip or heat the blanket and heat the liquid in the culture tank on the lateral wall of culture tank among the correlation technique, has not only saved the step of setting up heater strip or heat the blanket, is favorable to the state in producer's observation culture tank moreover, and the liquid preheating time in the culture tank is also shorter. Further, since the air at the top of the culture tank still contains a large amount of oxygen, the cost expenditure and the risk of contamination of additional supplemental oxygen can be reduced.
According to the bioreactor provided by the embodiment of the invention, the circulating air is used for heating the liquid in the culture tank, so that the dissolved oxygen of the culture liquid is increased, the oxygen consumption of the oxygen tank is reduced, and the cost is saved. And circulating air can play the mixed effect of cell and solution, need not use the stirring rake, has also solved and has adopted the stirring rake to carry out the mixture of solution and cell among the relevant art, however the stirring rake rotational speed is too fast can harm the cell, and the rotational speed is too slow culture solution and cell can not obtain the unable technical problem that lives of intensive mixing cell.
In addition, the bioreactor provided by the technical scheme of the invention also has the following additional technical characteristics:
in one possible design, the air extraction assembly includes: one end of the air pump is communicated with the air outlet of the culture tank; and the flow controller is communicated with the air pump and the gas heating assembly.
In the design, the air pumping assembly comprises an air pump and a flow controller, wherein one end of the air pump is communicated with the air outlet of the culture tank, and the flow controller is communicated with the air pump and the gas heating assembly. During the operation of the bioreactor, the air pump sucks air in the culture tank from the air outlet of the culture tank, and the air pump conveys the air in the culture tank to the flow controller, the flow controller conveys the air with the specific flow set by the user to the gas heating assembly, and the gas heating assembly discharges the gas with the specific temperature set by the user into the culture tank through the air inlet.
In one possible design, the bioreactor further comprises: and the vent pipe group is arranged in the culture tank, one end of the vent pipe group is communicated with the air inlet of the culture tank, and the vent pipe group is provided with an exhaust hole which is close to the bottom of the culture tank.
In the design, a vent pipe group is further arranged in the culture tank, one end of the vent pipe group is communicated with an air inlet of the culture tank, and an exhaust hole is formed in the vent pipe group, so that the gas at the specific temperature set by a user is connected into the vent pipe group in the culture tank through the air inlet by the gas heating component and then is exhausted into the culture tank through the exhaust hole. Further, the exhaust hole is close to the bottom of culture tank and is arranged, so that air can enter the bottom of the culture tank, and the liquid is located at the bottom of the culture tank, and the exhaust hole is arranged at the bottom of the culture tank, so that oxygen and other gases at the top of the culture tank can be fully dissolved in the culture solution at the bottom of the culture tank to promote the absorption of cells.
In one possible design, the vent tube set includes: the first end of the first breather pipe is communicated with the air inlet of the culture tank, and the second end of the first breather pipe extends towards the bottom of the culture tank; the second breather pipe is communicated with the second end of the first breather pipe, an included angle is formed between the second breather pipe and the first breather pipe, and the second breather pipe is provided with an exhaust hole.
In this design, the air pipe group includes first breather pipe and second breather pipe, and the first end of first breather pipe is linked together with the air inlet of cultivateing the jar, and the second end of first breather pipe extends towards the bottom of cultivateing the jar, and the second breather pipe is linked together with the second end of first breather pipe for the second breather pipe is located the lower position in the cultivation jar, is equipped with the exhaust hole on the second breather pipe. Make the air can directly enter into first breather pipe through the air inlet of culture tank, and then get into the second breather pipe that is arranged in culture tank lower position to discharge into culture tank through the exhaust hole that is arranged in on the second breather pipe, make the air can enter into culture tank's bottom position, because liquid is arranged in culture tank's bottom, set up the exhaust hole in culture tank's bottom, can be abundant dissolve oxygen and other gases at culture tank top in culture solution of culture tank bottom and promote the absorption of cell.
In one possible design, the bioreactor further comprises: the peristaltic pump is communicated with the culture tank and comprises a pump body and a hose, the hose can be filled when the pump body works, and air in the hose is emptied, so that liquid in the culture tank enters the hose.
In this design, bioreactor still includes the peristaltic pump that is linked together with the culture tank, can change the liquid in the culture tank automatically through the peristaltic pump, make bioreactor can change the culture solution of appointed dosage at the appointed time that the user set up, bioreactor liquid changing function in the correlation technique has all need the manual work to go on to have solved, increased the cost of labor and be difficult to avoid the technical problem of the experiment failure because of the artifical maloperation causes, and then realized that cell culture's in-process need not artificial intervention, the time of liquid changing is set for through user's input can realize the function of the automatic culture solution of changing of ration timing. Specifically, the peristaltic pump comprises a pump body and a hose, wherein the hose can be filled during the operation of the pump body, and air in the hose is emptied, so that liquid in the culture tank enters the hose, and then the liquid is discharged.
In one possible design, the hose has a diameter in the range of 1mm to 100 mm.
In this design, the diameter range of hose is 1mm to 100mm, and in this scope, the diameter undersize of having avoided the hose on the one hand, be less than 1mm and lead to peristaltic pump discharge liquid too slow to and the problem that the hose easily blockked up, on the other hand, the diameter of having also avoided the hose is too big, is greater than 100mm and leads to the too fast problem of peristaltic pump discharge liquid, makes the velocity of flow of peristaltic pump discharge liquid reasonable, makes the precision of peristaltic pump reasonable.
Furthermore, the hose is made of silicone tube, and the silicone has the advantages of no toxicity, difficult damage, long service life and the like.
In one possible design, the bioreactor further comprises: and a first temperature electrode provided in the culture tank, the first temperature electrode being capable of detecting a temperature of the liquid in the culture tank.
In this design, bioreactor is still including setting up the first temperature electrode in cultivateing the jar, and first temperature electrode can detect the liquid temperature who cultivates in the jar, through directly setting up first temperature electrode in cultivateing the jar to can accurate temperature in adjusting cultivateing the jar, reached more accurate, the effect of stable accuse temperature.
In one possible design, the bioreactor further comprises: and the pH electrode is arranged in the culture tank.
In this design, the bioreactor further comprises a pH electrode disposed in the culture tank, the pH electrode being capable of detecting the pH of the liquid in the culture tank. Further, install the PH meter at the top of culture tank, can observe the PH value of the interior liquid of culture tank in real time, conveniently in time adjust the PH value of the interior liquid of culture tank.
In one possible design, the bioreactor further comprises: and the second temperature electrode is arranged on the gas heating assembly.
In this design, the bioreactor further comprises a second temperature electrode disposed on the gas heating assembly, the second temperature electrode being capable of detecting the temperature of air heated by the gas heating assembly.
It can be understood that the cooperation of the second temperature electrode disposed on the gas heating assembly and the first temperature electrode disposed in the culture tank enables more precise temperature control.
In one possible design, the bioreactor further comprises: the connecting pipe is communicated with the gas outlet of the culture tank, the gas pumping assembly, the gas heating assembly and the gas inlet of the culture tank in sequence.
In this design, bioreactor still includes the connecting pipe, and the gas outlet of culture tank, the subassembly of bleeding, gaseous heating element and the air inlet of culture tank loop through the connecting pipe and link to each other, are convenient for realize culture tank, the connection between subassembly of bleeding and the gaseous heating element to, the connecting pipe can adopt materials such as silica gel, rubber to make, has the shape of easily changing, and advantages such as longe-lived are applicable to the connection between a plurality of objects.
In one possible design, the heating range of the gas heating assembly is 35 ℃ to 45 ℃.
In this design, gas heating element's heating range is 35 ℃ to 45 ℃, and in this within range, on the one hand, avoided the air temperature after the heating too high, be higher than 45 ℃ and lead to causing the damage to cell and culture solution, on the other hand, also avoided the temperature after the heating too low, lead to the problem that heating efficiency is low for the heating temperature scope is reasonable.
Additional aspects and advantages in accordance with the invention will be set forth in part in the description which follows and, in part, will be obvious from the description, or may be learned by practice of the invention.
Drawings
The above and/or additional aspects and advantages of the present invention will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings of which:
FIG. 1 shows a schematic structural view of a bioreactor according to an embodiment of the present invention.
In the figure:
100-a bioreactor; 110-culture tank; 112-an air inlet; 114-an air outlet; 120-air pump; 130-a flow controller; 140-a gas heating assembly; 150-a first vent pipe; 152-a second vent; 154-vent hole; 160-a first temperature electrode; 170-pH electrode; 180-connecting tube.
Detailed Description
In order that the above objects, features and advantages of the present invention can be more clearly understood, a more particular description of the invention will be rendered by reference to the appended drawings. It should be noted that the embodiments and features of the embodiments of the present application may be combined with each other without conflict.
In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention, however, the present invention may be practiced otherwise than as specifically described herein, and thus the scope of the present invention is not limited by the specific embodiments disclosed below.
A bioreactor 100 provided according to some embodiments of the present invention is described below with reference to fig. 1.
As shown in FIG. 1, an embodiment of one aspect of the present invention provides a bioreactor 100 comprising: a culture tank 110 provided with an air outlet 114 and an air inlet 112; a gas evacuation assembly in communication with gas outlet 114 of culture tank 110, the gas evacuation assembly configured to evacuate gas from culture tank 110; gas heating assembly 140 is in communication with a gas evacuation assembly at one end, which is capable of delivering gas from culture tank 110 to gas heating assembly 140, and at the other end is in communication with gas inlet 112 of culture tank 110.
The bioreactor 100 provided by one embodiment of the present invention comprises a culture tank 110, an air pumping assembly and a gas heating assembly 140, wherein the culture tank 110 is provided with an air outlet 114 and an air inlet 112, and the air pumping assembly is communicated with the air outlet 114 of the culture tank 110, so that the air pumping assembly can pump the gas in the culture tank 110; one end of gas heating module 140 is in communication with a gas evacuation module assembly capable of delivering gas from culture tank 110 to gas heating module 140, and the other end of gas heating module 140 is in communication with gas inlet 112 of culture tank 110. The air pumping assembly sucks air in the culture tank 110 from the air outlet 114 of the culture tank 110, and conveys the air in the culture tank 110 to the air heating assembly 140, and the air heating assembly 140 conveys air at a specific temperature set by a user into the culture tank 110 through the air inlet 112 of the culture tank 110, so that oxygen and other gases at the top of the culture tank 110 can be sufficiently dissolved in the culture solution at the bottom of the culture tank 110 to promote the absorption of cells. Moreover, the gas heating assembly 140 can heat the air to a suitable temperature and then feed the air into the culture tank 110, which not only saves the step of arranging a heating wire or a heating blanket, but also is beneficial for the production personnel to observe the state in the culture tank, and the preheating time of the liquid in the culture tank is shorter compared with the technical scheme that the heating wire or the heating blanket is wound on the side wall of the culture tank to heat the liquid in the culture tank in the related art. Further, since the air at the top of the culture tank 110 still contains a large amount of oxygen, the cost of additional oxygen supply and the risk of contamination can be reduced.
The bioreactor 100 provided by one embodiment of the present invention uses the circulating air to heat the liquid in the culture tank 110, so as to increase the dissolved oxygen of the culture solution, reduce the oxygen consumption of the oxygen tank and save the cost. And circulating air can play the mixed effect of cell and solution, need not use the stirring rake, has also solved and has adopted the stirring rake to carry out the mixture of solution and cell among the relevant art, however the stirring rake rotational speed is too fast can harm the cell, and the rotational speed is too slow culture solution and cell can not obtain the unable technical problem that lives of intensive mixing cell.
As shown in FIG. 1, in one embodiment of the present invention, the gas heating assembly 140 is a PTC heating module, a semiconductor heating module, or a heating wire, etc.
In one embodiment of the invention, the gas evacuation assembly comprises: an air pump 120 having one end connected to the outlet port 114 of the culture tank 110; and the flow controller 130 is communicated with the air pump 120 and the gas heating assembly 140.
In this embodiment, the air pumping assembly includes an air pump 120 and a flow controller 130, wherein one end of the air pump 120 is connected to the air outlet 114 of the culture tank 110, and the flow controller 130 is connected to the air pump 120 and the gas heating assembly 140. During the operation of bioreactor 100, air pump 120 sucks air in culture tank 110 from air outlet 114 of culture tank 110, and air pump 120 delivers air in culture tank 110 to flow controller 130, flow controller 130 delivers air at a specific flow rate set by the user to gas heating assembly 140, and gas heating assembly 140 discharges gas at a specific temperature set by the user into culture tank 110 through air inlet 112.
As shown in fig. 1, in one embodiment of the present invention, the bioreactor 100 further comprises: and a vent pipe group arranged in the culture tank 110, one end of the vent pipe group is communicated with the air inlet 112 of the culture tank 110, the vent pipe group is provided with an air outlet 154, and the air outlet 154 is arranged near the bottom of the culture tank 110.
In this embodiment, a vent tube set is further provided in the culture tank 110, one end of the vent tube set is communicated with the gas inlet 112 of the culture tank 110, and the vent tube set is provided with a gas outlet 154, so that the gas heating unit 140 introduces gas at a specific temperature set by a user into the vent tube set in the culture tank 110 through the gas inlet 112 and then discharges the gas into the culture tank 110 through the gas outlet 154. Further, the vent hole 154 is disposed near the bottom of the culture tank 110 so that air can enter the bottom of the culture tank 110, and since the liquid is located at the bottom of the culture tank 110, the vent hole 154 is disposed at the bottom of the culture tank 110 so that oxygen and other gases at the top of the culture tank 110 can be sufficiently dissolved in the culture liquid at the bottom of the culture tank 110 to promote the absorption of cells.
As shown in fig. 1, in one embodiment of the invention, a vent tubing set comprises: a first snorkel 150, a first end of the first snorkel 150 being in communication with the air inlet 112 of the culture tank 110, and a second end of the first snorkel 150 extending towards the bottom of the culture tank 110; and a second vent pipe 152 communicated with a second end of the first vent pipe 150, wherein an included angle is formed between the second vent pipe 152 and the first vent pipe 150, and a vent hole 154 is formed on the second vent pipe 152.
In this embodiment, the vent tube set includes a first vent tube 150 and a second vent tube 152, the first end of the first vent tube 150 is connected to the air inlet 112 of the culture tank 110, the second end of the first vent tube 150 extends toward the bottom of the culture tank 110, the second vent tube 152 is connected to the second end of the first vent tube 150, such that the second vent tube 152 is located at a lower position in the culture tank 110, and the second vent tube 152 is provided with a vent hole 154. Air can directly enter the first vent pipe 150 through the air inlet 112 of the culture tank 110 and then enter the second vent pipe 152 positioned at a lower position in the culture tank 110, and is discharged into the culture tank 110 through the vent hole 154 positioned on the second vent pipe 152, so that the air can enter the bottom position of the culture tank 110, and since the liquid is positioned at the bottom of the culture tank 110, the vent hole 154 is arranged at the bottom of the culture tank 110, oxygen and other gases at the top of the culture tank 110 can be sufficiently dissolved in the culture liquid at the bottom of the culture tank 110 to promote the absorption of cells.
In one embodiment, the first vent pipe 150 is vertically disposed in the culture tank 110, and the second vent pipe 152 is horizontally disposed in the culture tank 110, so that the first vent pipe 150 and the second vent pipe 152 have simple structures and are convenient to dispose, and the vent holes 154 on the second vent pipe 152 are located at lower positions in the culture tank 110, so as to sufficiently dissolve oxygen and other gases on the top of the culture tank 110 into the culture solution at the bottom of the culture tank 110 to promote the absorption of cells.
As shown in fig. 1, in one embodiment of the present invention, the bioreactor 100 further comprises: and the peristaltic pump is communicated with the culture tank 110 and comprises a pump body and a hose, wherein the pump body can be used for filling the hose and exhausting air in the hose so as to enable liquid in the culture tank 110 to enter the hose.
In this embodiment, bioreactor 100 still includes the peristaltic pump that is linked together with culture tank 110, can change the liquid in culture tank 110 automatically through the peristaltic pump, make bioreactor 100 can change the culture solution of appointed dosage at the appointed time that the user set up, bioreactor among the correlation technique of having solved trades the liquid function and all needs the manual work to go on, increased the cost of labor and be difficult to avoid the technical problem of the experiment failure because of artifical maloperation causes, and then realized that cell culture's in-process need not artificial intervention, the time of trading the liquid is set for through user's input can realize the function of the automatic culture solution of changing regularly of ration. Specifically, the peristaltic pump includes a pump body and a flexible tube, wherein the pump body is operable to fill the flexible tube and evacuate air from the flexible tube, thereby allowing liquid in the culture tank 110 to enter the flexible tube and thereby achieving liquid removal.
In one embodiment of the invention, the hose has a diameter in the range of 1mm to 100 mm.
In this embodiment, the diameter range of hose is 1mm to 100mm, and in this range, avoided the diameter undersize of hose on the one hand, be less than 1mm and lead to peristaltic pump discharge liquid too slow to and the easy problem of blockking up of hose, on the other hand, also avoided the diameter of hose too big, be greater than 100mm and lead to the too fast problem of peristaltic pump discharge liquid for the velocity of flow of peristaltic pump discharge liquid is reasonable, makes the precision of peristaltic pump reasonable.
Furthermore, the hose is made of silicone tube, and the silicone has the advantages of no toxicity, difficult damage, long service life and the like.
Of course, the present invention is not limited thereto, and it is understood that the diameter of the flexible tube can be adjusted to be slightly outside the range of 1mm to 100mm by those skilled in the art according to the design layout of the volume of the culture tank 110, and the specific situation is not illustrated here, but the present invention is within the protection scope of the present invention without departing from the design concept.
As shown in fig. 1, in one embodiment of the present invention, the bioreactor 100 further comprises: and a first temperature electrode 160 disposed in the culture tank 110, the first temperature electrode 160 being capable of detecting a temperature of a liquid in the culture tank 110.
In this embodiment, the bioreactor 100 further comprises a first temperature electrode 160 disposed in the culture tank 110, the first temperature electrode 160 can detect the temperature of the liquid in the culture tank 110, and the temperature in the culture tank 110 can be precisely adjusted by directly disposing the first temperature electrode 160 in the culture tank 110, so as to achieve the effects of more precise and stable temperature control.
As shown in fig. 1, in one embodiment of the present invention, the bioreactor 100 further comprises: and an alkalinity-acidity electrode disposed in the culture tank 110.
In this embodiment, bioreactor 100 further comprises a pH electrode disposed in culture tank 110, wherein the pH electrode is capable of detecting the pH of the liquid in culture tank 110. Further, install the PH meter at the top of culture tank 110, can observe the pH value of the interior liquid of culture tank 110 in real time, conveniently in time adjust the pH value of the interior liquid of culture tank 110.
As shown in fig. 1, in one embodiment of the present invention, the bioreactor 100 further comprises: and a second temperature electrode disposed on the gas heating assembly 140.
In this embodiment, bioreactor 100 further comprises a second temperature electrode disposed on gas heating assembly 140, the second temperature electrode being capable of detecting the temperature of air heated by gas heating assembly 140.
It will be appreciated that the cooperation of the second temperature electrode provided on the gas heating unit 140 and the first temperature electrode 160 provided in the culture tank 110 enables more precise temperature control.
As shown in fig. 1, in one embodiment of the present invention, the bioreactor 100 further comprises: the connecting pipe is communicated with the gas outlet 114 of the culture tank 110, the gas pumping assembly, the gas heating assembly 140 and the gas inlet 112 of the culture tank 110 in sequence.
In this embodiment, the bioreactor 100 further includes a connecting pipe, the air outlet 114 of the culture tank 110, the air pumping assembly, the gas heating assembly 140 and the air inlet 112 of the culture tank 110 are sequentially connected through the connecting pipe, which is convenient for realizing the connection among the culture tank 110, the air pumping assembly and the gas heating assembly 140, and the connecting pipe can be made of silica gel, rubber and other materials, and has the advantages of easy shape change, long service life and the like, and is suitable for the connection among a plurality of objects.
In one embodiment of the present invention, the heating range of the gas heating assembly 140 is 35 ℃ to 45 ℃.
In this embodiment, the heating range of the gas heating assembly 140 is 35 ℃ to 45 ℃, and in this range, on one hand, the air temperature after heating is too high and higher than 45 ℃ to cause damage to cells and culture solution, and on the other hand, the problem of low heating efficiency caused by too low temperature after heating is also avoided, so that the heating temperature range is reasonable.
It should be noted that, in the related art, the culture solution and the cells are mixed by using a stirring paddle, a heating blanket or a heating wire is wound around the culture tank to heat the culture solution, and air in an additional oxygen bottle or an air bottle is introduced into the culture tank. The related art has the following defects that the stirring speed is not adjustable, and the related art is not suitable for culturing adherent cells; cells are easily damaged when the stirring speed is too high, and the cell growth is influenced because the culture solution and the cells are not fully mixed due to too low stirring speed; separate gas cylinders, heating wires/blankets need to be connected, maintenance cost is high, and operation is complex. In the bioreactor 100 according to an embodiment of the present invention, as shown in fig. 1, the air in the culture tank 110 is sucked from the air outlet 114 of the culture tank 110 by the air suction assembly, and the air in the culture tank 110 is delivered to the air heating assembly 140, and the air heating assembly 140 delivers the air at the specific temperature set by the user to the culture tank 110 through the air inlet 112 of the culture tank 110, so that the oxygen and other gases at the top of the culture tank 110 can be sufficiently dissolved in the culture solution at the bottom of the culture tank 110 to promote the absorption of the cells. Moreover, the gas heating assembly 140 can heat the air to a suitable temperature and then feed the air into the culture tank 110, which not only saves the step of arranging a heating wire or a heating blanket, but also is beneficial for the production personnel to observe the state in the culture tank 110, and the preheating time of the liquid in the culture tank 110 is shorter compared with the technical scheme of winding the heating wire or the heating blanket on the side wall of the culture tank 110 to heat the liquid in the culture tank 110 in the related art. Further, since the air at the top of the culture tank 110 still contains a large amount of oxygen, the cost of additional oxygen supply and the risk of contamination can be reduced. Further, the liquid in the culture tank 110 is heated by using the circulating air, so that the dissolved oxygen of the culture solution is improved, the oxygen consumption of the oxygen tank is reduced, and the cost is saved. And circulating air can play the mixed effect of cell and solution, need not use the stirring rake, has also solved and has adopted the stirring rake to carry out the mixture of solution and cell among the relevant art, however the stirring rake rotational speed is too fast can harm the cell, and the rotational speed is too slow culture solution and cell can not obtain the unable technical problem that lives of intensive mixing cell.
In the related art, in order to heat the liquid in the culture tank, a heating jacket is generally wrapped around the outer wall of the culture tank, and in order to detect the temperature, a temperature sensor is mounted on the heating jacket, and a PID algorithm is used to control the heating time (turn on/off the heating jacket). However, the temperature of the heating jacket itself is often not the actual temperature in the culture tank. In order to accurately adjust the temperature in the tank, the invention installs the first temperature electrode 160 with high precision in the culture tank 110 to obtain the temperature in the culture tank 110 in real time, as shown in fig. 1, and simultaneously uses the pulse duty ratio (PWM) to achieve more accurate control of the power of the heating pipe. Thus, the temperature control systems inside and outside culture tank 110 cooperate to control, the temperature control system outside culture tank 110 performs rough adjustment, and the temperature control system inside culture tank 110 performs fine adjustment. The effects of more accuracy and stability are achieved.
In the description of the present specification, the terms "connect", "mount", "fix", and the like are to be understood in a broad sense, for example, "connect" may be a fixed connection, a detachable connection, or an integral connection; may be directly connected or indirectly connected through an intermediate. The specific meanings of the above terms in the present invention can be understood by those skilled in the art according to specific situations.
In the description herein, the description of the terms "one embodiment," "some embodiments," "specific embodiments," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The above is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes will occur to those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A bioreactor (100) comprising:
a culture tank (110) which is provided with an air outlet (114) and an air inlet (112);
a gas evacuation assembly in communication with a gas outlet (114) of the culture tank (110), the gas evacuation assembly configured to enable evacuation of gas within the culture tank (110);
and one end of the gas heating component (140) is communicated with the air pumping component, the air pumping component can convey the gas in the culture tank (110) to the gas heating component (140), and the other end of the gas heating component is communicated with the gas inlet (112) of the culture tank (110).
2. The bioreactor (100) of claim 1, wherein the air evacuation assembly comprises:
an air pump (120) having one end connected to the air outlet (114) of the culture tank (110);
a flow controller (130) communicating the air pump (120) and the gas heating assembly (140).
3. The bioreactor (100) of claim 1, further comprising:
and the aeration pipe group is arranged in the culture tank (110), one end of the aeration pipe group is communicated with the air inlet (112) of the culture tank (110), the aeration pipe group is provided with an exhaust hole (154), and the exhaust hole (154) is arranged close to the bottom of the culture tank (110).
4. The bioreactor (100) of claim 3, wherein the set of aeration tubes comprises:
a first vent tube (150), a first end of the first vent tube (150) being in communication with the air inlet (112) of the culture tank (110), a second end of the first vent tube (150) extending towards the bottom of the culture tank (110);
the second breather pipe (152) is communicated with the second end of the first breather pipe (150), an included angle is formed between the second breather pipe (152) and the first breather pipe (150), and the second breather pipe (152) is provided with the exhaust hole (154).
5. The bioreactor (100) according to any one of claims 1 to 4, further comprising:
the peristaltic pump is communicated with the culture tank (110) and comprises a pump body and a hose, wherein the pump body can be used for filling the hose and emptying air in the hose so as to enable liquid in the culture tank (110) to enter the hose.
6. The bioreactor (100) of claim 5,
the hose has a diameter in the range of 1mm to 100 mm.
7. The bioreactor (100) according to any one of claims 1 to 4, further comprising:
a first temperature electrode (160) provided in the culture tank (110), the first temperature electrode (160) being capable of detecting a temperature of a liquid in the culture tank (110); and/or
And the pH value electrode (170) is arranged in the culture tank (110).
8. The bioreactor (100) according to any one of claims 1 to 4, further comprising:
a second temperature electrode disposed on the gas heating assembly (140).
9. The bioreactor (100) according to any one of claims 1 to 4, further comprising:
and the connecting pipe (180) is sequentially communicated with the gas outlet (114) of the culture tank (110), the gas pumping component, the gas heating component (140) and the gas inlet (112) of the culture tank (110).
10. The bioreactor (100) according to any one of claims 1 to 4,
the heating range of the gas heating assembly (140) is 35 ℃ to 45 ℃.
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| CN202122089823.4U CN215799630U (en) | 2021-08-31 | 2021-08-31 | Bioreactor |
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| CN202122089823.4U CN215799630U (en) | 2021-08-31 | 2021-08-31 | Bioreactor |
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| CN215799630U true CN215799630U (en) | 2022-02-11 |
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