CN113797246A

CN113797246A - Traditional Chinese medicine composition and preparation method and application thereof

Info

Publication number: CN113797246A
Application number: CN202111253425.XA
Authority: CN
Inventors: 刘红旭; 周明学; 龙德怀; 尚菊菊; 李思耐; 邢文龙
Original assignee: BEIJING INSTITUTE OF TRADITIONAL CHINESE MEDICINE; Beijing Traditional Chinese Medicine Hospital
Current assignee: BEIJING INSTITUTE OF TRADITIONAL CHINESE MEDICINE; Beijing Traditional Chinese Medicine Hospital; Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital University of Medicine Sciences
Priority date: 2021-10-27
Filing date: 2021-10-27
Publication date: 2021-12-17

Abstract

The invention relates to the technical field of traditional Chinese medicines, in particular to a traditional Chinese medicine composition and a preparation method and application thereof. The traditional Chinese medicine composition comprises the following components in parts by weight: 10-50 parts of angelica sinensis, 10-50 parts of ligusticum wallichii, 10-50 parts of caulis spatholobi, 3-17 parts of rhizoma corydalis and 1-5 parts of pseudo-ginseng. The Chinese medicinal composition adopts angelica sinensis, ligusticum wallichii and caulis spatholobi as monarch and minister medicaments, and rhizoma corydalis and pseudo-ginseng as assistant and guide medicaments, has the effects of nourishing blood, promoting blood circulation, removing blood stasis and relieving pain by scientific proportioning of various medicament components and synergistic cooperation of the components, and has good treatment effect on myocardial injury and cerebral thrombosis and small toxic and side effects.

Description

Traditional Chinese medicine composition and preparation method and application thereof

Technical Field

The invention relates to the technical field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine composition and a preparation method and application thereof.

Background

At present, diseases such as myocardial ischemia, myocardial injury, angina pectoris, coronary heart disease, cerebral thrombosis and the like are main diseases which harm human health, and the incidence rate and the death rate of the diseases are high, particularly myocardial injury and cerebral thrombosis.

Cerebral thrombosis is a common ischemic cerebrovascular disease, and is mainly caused by the fact that blood coagulation factors are coagulated into blocks in a lumen due to multiple reasons such as arteriosclerosis, intimal injury of a blood vessel, rough intima of the lumen, narrowing of the lumen and the like to form thrombus, so that the blood vessel is blocked and blood flow is interrupted, and cerebral tissues in a blood supply area of the blood vessel are caused to suffer from ischemia, anoxia, softening and necrosis. Cerebral thrombosis can occur in any section of cerebral blood vessel, but is clinically common with thrombosis formed by branches of internal carotid artery, anterior cerebral artery and middle cerebral artery. Cerebral thrombosis has great harm to the body and seriously affects the daily life of patients. Mild cerebral thrombosis is manifested by one side of the body being inflexible, slow feeling, loss of speech, severe or even death.

Myocardial damage refers to damage to myocardial cells caused by various causes, i.e., myocardial cell necrosis, apoptosis, and the like. Myocardial damage may be caused by ischemic diseases, such as coronary heart disease, myocardial infarction, etc., by inflammation, such as myocarditis, etc., or by trauma, such as puncturing the myocardium, etc. Myocardial damage is often manifested as chest distress, chest pain, dyspnea, fatigue, nausea, severe acute heart failure, arrhythmia and even sudden death. Because of the many causes of myocardial damage and the complex pathological mechanism, the treatment of myocardial damage has been receiving attention, especially isoproterenol induced myocardial damage, which can induce the above mentioned various types of myocardial damage.

At present, the treatment of myocardial injury and cerebral thrombosis mainly comprises two treatment methods of traditional Chinese medicine and western medicine, the side effect of the medicine for the western medicine treatment is large, and the medicine can treat the injury of different degrees to human organs after long-term use; the traditional Chinese medicines have small toxic and side effects, but most of the medicines have slow acting time, long taking period and unsatisfactory drug effect, so that patients can give up treatment easily.

In view of the above, the present invention is particularly proposed.

Disclosure of Invention

The first purpose of the invention is to provide a traditional Chinese medicine composition which has the effects of nourishing blood, promoting blood circulation, removing blood stasis and relieving pain and has good treatment effect on myocardial injury and cerebral thrombosis.

The second objective of the present invention is to provide a preparation method of the above-mentioned traditional Chinese medicine composition, which is simple to operate.

The third purpose of the invention is to provide an application of the traditional Chinese medicine composition in preparing a medicine for treating myocardial injury.

The fourth purpose of the invention is to provide an application of the traditional Chinese medicine composition in preparing a medicine for treating cerebrovascular diseases.

In order to achieve the above purpose of the present invention, the following technical solutions are adopted:

the invention provides a traditional Chinese medicine composition which comprises the following components in parts by weight: 10-50 parts of angelica sinensis, 10-50 parts of ligusticum wallichii, 10-50 parts of caulis spatholobi, 3-17 parts of rhizoma corydalis and 1-5 parts of pseudo-ginseng.

The invention also provides a preparation method of the traditional Chinese medicine composition, which is characterized in that angelica, ligusticum wallichii, caulis spatholobi and rhizoma corydalis are added with water for decoction, and pseudo-ginseng is added and mixed evenly to obtain traditional Chinese medicine mixed liquid.

The invention also provides application of the traditional Chinese medicine composition in preparing a medicine for treating myocardial injury.

The invention also provides application of the traditional Chinese medicine composition in preparing a medicine for treating cerebrovascular diseases.

Compared with the prior art, the invention has the beneficial effects that:

(1) the invention provides a traditional Chinese medicine composition, which is prepared by selecting raw materials according to the theory of traditional Chinese medicine and scientifically proportioning the traditional Chinese medicine components, so that the drug effects of various components are mutually matched and have synergistic effect. According to the holistic concept of the traditional Chinese medicine, the advantages of dialectical treatment of the traditional Chinese medicine are exerted, the treatment is carried out aiming at different causes and multiple angles, and the deficiency and the excess are combined to treat both principal and secondary aspects of diseases. The traditional Chinese medicine composition consists of angelica, ligusticum wallichii, caulis spatholobi, rhizoma corydalis and pseudo-ginseng. Wherein, the angelica, the hemlock parsley and the suberect spatholobus stem belong to the blood nourishing and blood circulation promoting class, and are used as monarch and ministerial drugs; rhizoma corydalis and pseudo-ginseng belong to the category of stasis removing and stasis dissipating, and are lightly used as adjuvant and guiding drugs, so that the rhizoma corydalis and pseudo-ginseng have the effects of nourishing blood, activating blood, removing stasis and relieving pain, and have obvious treatment effects on myocardial injury and cerebral thrombosis. Compared with the current chemotherapeutics for treating myocardial injury or cerebral thrombosis, the traditional Chinese medicine composition provided by the invention is stable in efficacy, is a natural pure plant medicine, only contains 5 traditional Chinese medicine components, and has the advantages of remarkably reduced adverse reaction and side effect caused by the medicine and small toxic and side effects.

(2) The invention provides a preparation method of the traditional Chinese medicine composition, and the method has the advantages of easily obtained raw materials, simple and convenient preparation process, simple operation and easy popularization and application.

(3) The invention provides the application of the traditional Chinese medicine composition in preparing the medicine for treating myocardial injury, and the traditional Chinese medicine composition has obvious improvement effect on myocardial apoptosis and myocardial ischemia.

(4) The invention provides application of the traditional Chinese medicine composition in preparing a medicine for treating cerebrovascular diseases, particularly has an obvious treatment effect on cerebral thrombosis, and can obviously reduce the incidence rate of the cerebral thrombosis and the area of the cerebral thrombosis.

Drawings

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings that are required to be used in the embodiments will be briefly described below, it should be understood that the following drawings only illustrate some embodiments of the present invention and therefore should not be considered as limiting the scope, and for those skilled in the art, other related drawings can be obtained according to the drawings without inventive efforts.

FIG. 1 is a phenotype diagram of isoproterenol-induced myocardial apoptosis in zebrafish.

FIG. 2 shows the effect of the mixed solution of Chinese herbs on the fluorescence intensity of apoptotic cells of zebra fish heart.

FIG. 3 shows the effect of the mixture of Chinese medicinal materials on the improvement of the apoptosis rate of myocardial cells of zebra fish.

FIG. 4 shows the effect of Chinese medicinal mixture on myocardial ischemia cardiac output of zebra fish.

FIG. 5 shows the effect of the Chinese medicinal mixture on the cardiac output increase rate of zebra fish myocardial ischemia.

FIG. 6 shows the effect of the Chinese medicinal mixture on the blood flow velocity of myocardial ischemia in zebra fish.

FIG. 7 shows the effect of the Chinese medicinal mixture on the increase rate of blood flow velocity of myocardial ischemia in zebra fish.

FIG. 8 shows the effect of Chinese medicinal mixture on the cerebral thrombus area of zebra fish.

Detailed Description

The technical solutions of the present invention will be described clearly and completely with reference to the following detailed description, but those skilled in the art will understand that the following described examples are some, not all, of the examples of the present invention, and are only used for illustrating the present invention, and should not be construed as limiting the scope of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention. The examples, in which specific conditions are not specified, were conducted under conventional conditions or conditions recommended by the manufacturer. The reagents or instruments used are not indicated by the manufacturer, and are all conventional products available commercially.

The following provides a detailed description of the Chinese medicinal composition of the embodiments of the present invention, its preparation method and application.

Some embodiments of the present invention provide a traditional Chinese medicine composition, which comprises the following components in parts by weight: 10-50 parts of angelica sinensis, 10-50 parts of ligusticum wallichii, 10-50 parts of caulis spatholobi, 3-17 parts of rhizoma corydalis and 1-5 parts of pseudo-ginseng.

The Chinese angelica is named as dry angelica, Qinlian, Xi angelica, Min angelica, golden angelica, Yun Gui Tai, Dang Gui Wei, angelica koji and Tu angelica, and can be used as a medicine for many years; warm in nature, sweet and pungent in flavor; it enters liver, heart and spleen meridians; has effects in replenishing blood, promoting blood circulation, regulating menstruation, relieving pain, and loosening bowel to relieve constipation. Can be used for treating blood deficiency, blood stasis, vertigo, headache, palpitation, numbness of limbs, menoxenia, amenorrhea, dysmenorrhea, metrorrhagia, abdominal pain, debility, dysentery, constipation, traumatic injury, swelling and pain, carbuncle, and pyocutaneous disease.

Rhizoma Ligustici Chuanxiong is Umbelliferae plant, perennial herb; warm in nature and pungent in flavor; it enters liver, gallbladder and heart meridians; has effects in promoting blood circulation, activating qi-flowing, dispelling pathogenic wind, and relieving pain. It can be used for treating wind cold headache, dizziness, hypochondriac pain, abdominal pain, arthralgia due to cold, spasm of tendons, amenorrhea, dystocia, puerperal stagnation, pain, superficial infection, skin ulcer, etc.

Caulis Spatholobi is a different name of multiple plants of Leguminosae, angiosperma, Dicotyledoneae, Papilionaceae, etc.; warm in nature, bitter and slightly sweet in taste; heart and spleen meridians entered; has effects in promoting blood circulation, relaxing muscles and tendons, nourishing blood, and regulating menstruation. It can be used for treating numbness of hand and foot, rheumatic arthralgia, menoxenia, dysmenorrhea, and amenorrhea.

Rhizoma corydalis and rhizoma corydalis are also known as rhizoma corydalis and rhizoma corydalis, and are perennial herb of corydalis of Papaveraceae; warm in nature, pungent and bitter in flavor; entering heart, spleen, liver and lung; has effects of promoting blood circulation, removing blood stasis, activating qi-flowing and relieving pain. Can be used for treating pain due to qi stagnation and blood stasis, dysmenorrhea, amenorrhea, abdominal mass, puerperal stagnation, traumatic injury, and hernia pain.

Notoginseng radix is plant of Araliaceae; sweet, slightly bitter and warm in nature; entering liver and stomach meridians; has hemostatic, blood circulation promoting, blood stasis dispelling, repercussive, and analgesic effects. Can be used for treating traumatic injury, blood stasis, swelling, thoracic obstruction, angina pectoris, amenorrhea, dysmenorrhea, puerperal yin and abdominal pain, skin ulcer, carbuncle, and swelling and pain.

The whole formula consists of angelica, hemlock parsley, suberect spatholobus stem, rhizoma corydalis and pseudo-ginseng. The angelica, the ligusticum wallichii and the caulis spatholobi belong to the blood nourishing and blood circulation promoting class, and are used as monarch and ministerial drugs; rhizoma corydalis and notoginseng are classified as blood stasis-resolving and stasis-removing herbs, and are used as adjuvant and guiding drugs. The whole formula has the main effects of nourishing blood and activating blood, and removing blood stasis and relieving pain as auxiliary effects, and has good treatment effects on myocardial injury and cerebral thrombosis.

Through scientific proportioning of the traditional Chinese medicine components, the drug effects of various components are mutually matched and have synergistic effect, particularly the usage of the caulis spatholobi and the rhizoma corydalis; spatholobus stem is called as the holy medicine of blood system, and spatholobus stem and rhizoma corydalis are mainly in the liver, spleen and kidney meridians, and are in the blood system, so they are more consistent with the physiological characteristics of heart governing blood vessels. The caulis spatholobi is matched with the rhizoma corydalis to dredge the collaterals, and simultaneously, the blood circulation is also promoted to relieve pain, thus embodying the principle of treating both principal and secondary aspect of disease. The raw materials in the traditional Chinese medicine composition are all plant medicines, and only 5 traditional Chinese medicine components are used, so that the raw materials are fewer, the side effect of the medicine is obviously reduced, and the toxicity is low.

In order to obtain a traditional Chinese medicine composition with more excellent treatment effect, the proportion relation of the raw materials in the traditional Chinese medicine composition is optimized, and the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 25-40 parts of angelica, for example, but not limited to, 25 parts, 28 parts, 30 parts, 33 parts, 35 parts, 38 parts or 40 parts; 25-40 parts of ligusticum wallichii, such as, but not limited to, 25 parts, 28 parts, 30 parts, 33 parts, 35 parts, 38 parts or 40 parts; 25-40 parts of caulis spatholobi, such as but not limited to 25 parts, 28 parts, 30 parts, 33 parts, 35 parts, 38 parts or 40 parts; the weight portions of rhizoma corydalis are 8-13, such as but not limited to 8, 9, 10, 11, 12 or 13; and 2.5 to 4 parts of pseudo-ginseng, for example, but not limited to, 2.5 parts, 3 parts, 3.5 parts or 4 parts.

Specifically, in some embodiments, the traditional Chinese medicine composition comprises the following components in parts by weight: 30 parts of angelica, 30 parts of ligusticum wallichii, 30 parts of caulis spatholobi, 10 parts of rhizoma corydalis and 3 parts of pseudo-ginseng.

In some embodiments, the above-mentioned Chinese medicinal composition further comprises an adjuvant for preparing the Chinese medicinal composition into various pharmaceutical dosage forms.

In the present invention, the dosage form of the Chinese medicinal preparation is not strictly limited, and the dosage form includes decoction, granule, tablet, pill, drink tablet or capsule.

The invention also provides a preparation method of the traditional Chinese medicine composition, which is characterized in that angelica, ligusticum wallichii, caulis spatholobi and rhizoma corydalis are decocted by adding water, and pseudo-ginseng is added for mixing to obtain a traditional Chinese medicine mixed solution.

Specifically, in some embodiments, the preparation method of the mixed solution of the traditional Chinese medicines comprises: adding 3-6 times of water into angelica, ligusticum wallichii, caulis spatholobi and rhizoma corydalis, heating until the water is boiled, decocting for 10-30 min, filtering to obtain filtrate, adding pseudo-ginseng, and mixing uniformly to obtain the traditional Chinese medicine mixed solution. The mass of the added water is 3-6 times of the total mass of the angelica, the ligusticum wallichii, the suberect spatholobus stem and the rhizoma corydalis. Preferably, the pseudo-ginseng is pseudo-ginseng powder.

Through the limitation of the parameters and the specific limitation of each step, the full release of the effective components in the traditional Chinese medicine raw materials can be improved, and the treatment effect of the prepared traditional Chinese medicine composition is favorably improved.

Preferably, the myocardial injury comprises one or both of apoptosis of myocardial cells and ischemia of myocardium.

The traditional Chinese medicine composition has obvious improvement effect on cardiac muscle cell apoptosis and cardiac muscle ischemia caused by isoproterenol; has obvious effect of increasing cardiac output reduction and blood flow speed reduction caused by isoproterenol.

In some embodiments, the cerebrovascular disease is cerebral thrombosis. The traditional Chinese medicine composition can reduce the incidence of cerebral thrombosis and the area of cerebral thrombosis, and has an obvious treatment effect on the cerebral thrombosis.

The treated object of the traditional Chinese medicine composition comprises human or animals, preferably, the animals comprise fish.

Example 1

The embodiment provides a traditional Chinese medicine composition, which comprises the following components in parts by weight: 10g of angelica, 10g of ligusticum wallichii, 10g of caulis spatholobi, 3.3g of rhizoma corydalis and 1g of pseudo-ginseng.

Adding 400mL of water into the angelica, the ligusticum wallichii, the caulis spatholobi and the rhizoma corydalis, heating until the water is boiled, decocting for 20min, filtering to obtain filtrate, adding the pseudo-ginseng powder, and mixing uniformly to obtain a traditional Chinese medicine mixed solution.

Example 2

The embodiment provides a traditional Chinese medicine composition, which comprises the following components in parts by weight: 50g of angelica, 50g of ligusticum wallichii, 50g of caulis spatholobi, 17g of rhizoma corydalis and 5g of pseudo-ginseng.

The preparation method of the mixed solution of Chinese herbs in this example is the same as that of example 1.

Example 3

The embodiment provides a traditional Chinese medicine composition, which comprises the following components in parts by weight: 25g of angelica, 25g of ligusticum wallichii, 25g of caulis spatholobi, 8g of rhizoma corydalis and 2.5g of pseudo-ginseng.

Example 4

The embodiment provides a traditional Chinese medicine composition, which comprises the following components in parts by weight: 40g of angelica, 40g of ligusticum wallichii, 40g of caulis spatholobi, 13g of rhizoma corydalis and 4g of pseudo-ginseng.

Example 5

The embodiment provides a traditional Chinese medicine composition, which comprises the following components in parts by weight: 35g of angelica, 35g of ligusticum wallichii, 35g of suberect spatholobus stem, 12g of rhizoma corydalis and 4g of pseudo-ginseng.

Example 6

The embodiment provides a traditional Chinese medicine composition, which comprises the following components in parts by weight: 30g of angelica, 30g of ligusticum wallichii, 30g of caulis spatholobi, 10g of rhizoma corydalis and 3g of pseudo-ginseng.

Test examples

The pharmacodynamic evaluation of the traditional Chinese medicine reagent provided in example 6 of the present invention is performed by combining the pharmacodynamic test as follows:

test example 1, effect of Chinese medicinal mixture solution on improving myocardial apoptosis.

The traditional Chinese medicine mixed solution in the embodiment 6 is diluted by water into traditional Chinese medicine mixed solution (also called pseudo-ginseng traditional Chinese medicine mixed solution or traditional Chinese medicine decoction) with the concentration of 8.3 muL/mL, 25 muL/mL and 75 muL/mL respectively.

Randomly selecting 180-tail wild AB-series zebra fishes 2 days after fertilization (2dpf) in a six-hole plate, treating 30-tail zebra fishes in each hole, wherein the volume of each hole is 3mL, respectively adding 3mL of Chinese medicinal mixed liquor (experimental group 1-3) with the concentration of 8.3 muL/mL, 25 muL/mL and 75 muL/mL, and dripping pills of the red sage root into 500 mug/mL (positive control group), and setting a normal control group (zebra fishes treated by water for fish culture) and a model control group. After the zebra fish is treated by the drug for 4 hours, 3mL of isoproterenol hydrochloride with the concentration of 5mg/mL is used for inducing for 60min to establish a zebra fish myocardial ischemia model, after the drug and the inducer are jointly treated, acridine orange (AO for short) is used for dyeing, 10 zebra fish in each group are randomly selected after the dyeing is finished and observed and photographed under a fluorescence microscope, and the result is shown in figure 1. Data are collected, and the fluorescence intensity of the apoptotic cells at the heart of the zebra fish is analyzed and counted, and the result is shown in figure 2. The improvement effect of the traditional Chinese medicine mixed solution on the apoptosis of the myocardial cells of the zebra fish is evaluated according to the statistical significance of the fluorescence intensity of the apoptotic cells at the heart, and the result is shown in figure 3. The fluorescence intensity of the traditional Chinese medicine mixed solution on the zebra fish heart apoptotic cells and the improvement rate of the myocardial apoptosis are recorded in table 1. Statistical analysis using analysis of variance and Dunnett's T-test indicated significant differences with p < 0.05. Wherein, the calculation formula of the myocardial cell apoptosis improvement rate is as follows:

TABLE 1

P <0.05, p <0.001, compared to model control group

Comparing the fluorescence intensity (307264 pixel) of the model control group zebra fish heart apoptotic cell with the normal control group (117837 pixel) p is less than 0.001, which indicates that the model is successfully established. The fluorescence intensity of the apoptotic cells at the heart part of the zebra fish of the positive control group (the salvia miltiorrhiza dropping pill is 500 mu g/mL) is 251615 pixels, compared with the model control group, the p is less than 0.05, the myocardial apoptosis improvement rate is 29 percent, and the salvia miltiorrhiza dropping pill shows that the salvia miltiorrhiza dropping pill has obvious improvement effect on the myocardial apoptosis of the zebra fish with myocardial ischemia. The concentration of the traditional Chinese medicine mixed liquor is respectively 8.3 mu L/mL of the experimental group 1, the fluorescence intensity of the apoptotic cells at the heart part of the zebra fish is 316590 pixels, compared with a model control group (307264 pixels), p is more than 0.05, the apoptosis improvement rate of the myocardial cells is-5%, and the traditional Chinese medicine mixed liquor with the concentration of 8.3 mu L/mL cannot improve the apoptosis of the myocardial cells of the zebra fish with myocardial ischemia. The concentrations of the traditional Chinese medicine mixed liquor are respectively 25 mu L/mL and 75 mu L/mL of the experimental group 2 and the experimental group 3, the fluorescence intensities of the apoptotic cells at the heart part of the zebra fish are 242615 pixels and 204233 pixels respectively, compared with a model control group (307264 pixel), the improvement rates of the apoptosis of the myocardial cells are 34% and 54% respectively when p is less than 0.05 and p is less than 0.001 respectively, and the obvious improvement effect is shown on the apoptosis of the myocardial cells of the zebra fish caused by the traditional Chinese medicine mixed liquor isoproterenol with the concentrations of 25 mu L/mL and 75 mu L/mL.

Test example 2, the effect of the Chinese medicinal mixture on cardiac output and blood flow rate.

The traditional Chinese medicine reagent in the embodiment 6 is diluted by water into traditional Chinese medicine mixed solution (also called pseudo-ginseng traditional Chinese medicine mixed solution) with the concentration of 8.3 mu L/mL, 25 mu L/mL and 75 mu L/mL respectively. And randomly selecting 180 wild type AB line zebra fishes 3 days (3dpf) after fertilization in a six-hole plate, and treating 30 zebra fishes in each hole, wherein the volume of each hole is 3 mL. Respectively administering Chinese medicinal mixed solution (experimental group 1-3) with concentration of 8.3 μ L/mL, 25 μ L/mL and 75 μ L/mL, and Saviae Miltiorrhizae radix dripping pill 500 μ g/mL (positive control group), and setting normal control group (water-treated zebra fish for fish culture) and model control group. After the zebra fish is treated by the drug for 4 hours, the zebra fish is induced by 50mg/mL isoproterenol hydrochloride for 60min to establish a zebra fish myocardial ischemia model, after the drug and the inducer are treated together, the zebra fish is placed under a heartbeat and blood flow analysis system to record a zebra fish blood flow video, the zebra fish heart output is analyzed, the result is shown in figure 4, and the heart output increase rate is shown in figure 5. And data of cardiac output and rate of increase in cardiac output were recorded in table 2. The blood flow velocity of zebra fish was analyzed, and the results are shown in fig. 6, and the blood flow velocity increase rate is shown in fig. 7. And data of cardiac output and rate of increase in cardiac output were recorded in table 3. The protective effect of the traditional Chinese medicine mixed solution on the myocardial ischemia of the zebra fish induced by the isoproterenol hydrochloride is quantitatively evaluated according to the cardiac output and blood flow rate indexes. Statistical analysis using analysis of variance and Dunnett's T-test indicated significant differences with p < 0.05. Wherein, the calculation formula of the cardiac output increase rate and the blood flow velocity increase rate is as follows:

TABLE 2

P <0.01, p <0.001, compared to model control group

Comparing the cardiac output (0.10nL/s) of the zebra fish in the model control group with that in the normal control group (0.39nL/s) by p <0.001, which indicates that the model is successfully established. The cardiac output of the zebra fish of the positive control group (the salvia miltiorrhiza dropping pill is 500 mu g/mL) is 0.23nL/s, compared with the model control group, the p is less than 0.01, the cardiac output increase rate is 45 percent, and the salvia miltiorrhiza dropping pill has obvious improvement effect on the cardiac output of the zebra fish with myocardial ischemia. The concentrations of the traditional Chinese medicine mixed liquor are respectively 8.3 muL/mL, 25 muL/mL and 75 muL/mL, the cardiac output of the zebra fish is respectively 0.25nL/s, 0.37nL/s and 0.28nL/s, compared with a model control group, the cardiac output is p <0.001, and the cardiac output increase rates are respectively 52%, 93% and 62%. The traditional Chinese medicine mixed liquor with the concentrations of 8.3 muL/mL, 25 muL/mL and 75 muL/mL respectively has obvious increasing effect on the cardiac output reduction of the zebra fish caused by isoproterenol, wherein the traditional Chinese medicine mixed liquor with the concentration of 25 muL/mL has more excellent increasing effect on the cardiac output reduction of the zebra fish caused by isoproterenol.

TABLE 3

P <0.01, p <0.001, compared to model control group

The comparison of the blood flow velocity (293 μm/s) of the zebra fish in the model control group with that in the normal control group (931 μm/s) proves that the model is successfully established, wherein p is less than 0.001. The blood flow velocity (628 mu m/s) of the zebra fish in the positive control group (500 mu g/mL of the salvia miltiorrhiza dropping pill) is less than 0.01 compared with that in the model control group, and the blood flow velocity increase rate is 36 percent, which shows that the salvia miltiorrhiza dropping pill has obvious increase effect on the blood flow velocity of the zebra fish with myocardial ischemia. The concentrations of the traditional Chinese medicine mixed liquor are respectively 8.3 mu L/mL, 25 mu L/mL and 75 mu L/mL, the blood flow velocities of the zebra fish are 564 mu m/s, 791 mu m/s and 587 mu m/s, compared with a model control group, the blood flow velocities are p <0.001, and the blood flow velocity increase rates are respectively 29%, 53% and 32%. The results show that the traditional Chinese medicine mixed liquor with the concentrations of 8.3 muL/mL, 25 muL/mL and 75 muL/mL respectively has obvious increase effect on the blood flow speed reduction of the zebra fish caused by isoproterenol.

By combining the experimental results, under the condition of the experimental concentration, the traditional Chinese medicine mixed liquor has obvious improvement effect on the apoptosis of the cardiac muscle of the zebra fish caused by isoproterenol; has obvious effect of increasing the cardiac output reduction and the blood flow speed reduction of the zebra fish caused by isoproterenol.

Test example 3, effect of the Chinese medicinal mixture on improving cerebral thrombosis of zebra fish.

The traditional Chinese medicine mixed solution in the embodiment 6 is diluted by water into traditional Chinese medicine mixed solution (also called pseudo-ginseng traditional Chinese medicine mixed solution or traditional Chinese medicine decoction) with the concentration of 1.74 mu L/mL, 5.21 mu L/mL and 15.6 mu L/mL respectively.

Randomly selecting 2dpf melanin allele mutant zebra fish in a 6-well plate, treating 30 zebra fish in each well, wherein the volume of each well is 3mL, respectively administering traditional Chinese medicine mixed liquor (experiment group 1-3) with the concentration of 1.74 mu L/mL, 5.21 mu L/mL and 15.6 mu L/mL, aspirin (positive control group) with the concentration of 50.0 mu g/mL, and simultaneously setting a normal control group and a model control group. Except for the normal control group, all the other groups are dissolved in water and are given with ponatinib to establish a zebra fish brain thrombus model. After 18h of treatment at 28 ℃ the fish were stained with o-dianisidine and after staining all zebrafish were photographed under a dissecting microscope, the results of which are shown in fig. 8. Counting the number (N) of the zebra fish brain thrombi, calculating the incidence rate of the zebra fish brain thrombi, evaluating the prevention and treatment efficacy of the traditional Chinese medicine mixed liquor according to the statistical analysis result of the index, and calculating the improvement rate of the cerebral thrombosis, wherein the results are shown in Table 4. Statistical treatment results are expressed as mean ± SE. Statistical analysis was performed using the Fisher's exact test, and p <0.05 indicated significant differences.

The good rate of cerebral thrombosis is calculated according to the following formula:

analyzing and collecting data by software, analyzing the cerebral thrombus area of the zebra fish, evaluating the cerebral thrombus prevention and treatment effect of the Chinese medicinal mixed solution according to the statistical analysis result of the index, and calculating the improvement rate of the cerebral thrombus area, wherein the result is shown in fig. 5. Statistical treatment results are expressed as mean ± SE. Statistical analysis was performed using SPSS26.0 software and p <0.05 indicated that the differences were statistically significant.

TABLE 4

In the experimental group 1 with the concentration of the traditional Chinese medicine mixed solution of 1.74 mu L/mL, the incidence rate of the zebra fish cerebral thrombosis is 66.7%, and compared with the model control group (the incidence rate of the cerebral thrombosis is 66.7%), the cerebral thrombosis prevention and treatment rates are respectively 0, which indicates that the traditional Chinese medicine mixed solution with the concentration of 1.74 mu L/mL cannot play the role of preventing and treating the cerebral thrombosis. The incidence rate of the zebra fish cerebral thrombosis is 56.7% in the experimental group 2 with the concentration of the traditional Chinese medicine mixed liquor of 5.21 mu L/mL respectively, and compared with the model control group (the incidence rate of the cerebral thrombosis is 66.7%), the cerebral thrombosis prevention rate is 15%, which shows that the traditional Chinese medicine mixed liquor has a certain improvement effect on the zebra fish cerebral thrombosis. The incidence of the cerebral thrombosis of the zebra fish in the experimental group 3 with the concentration of the traditional Chinese medicine mixed liquor of 15.6 mu L/mL respectively is 40 percent, compared with the P of a model control group (the incidence of the cerebral thrombosis is 66.7 percent), the prevention and treatment rate of the cerebral thrombosis is 40 percent, and the cerebral thrombosis has obvious improvement effect on the cerebral thrombosis of the zebra fish.

TABLE 5

P <0.05, p <0.01, compared to model control group

The concentrations of the traditional Chinese medicine mixed liquor are respectively 1.74 mu L/mL, 5.21 mu L/mL and 15.6L/mL, the fluorescence intensities of apoptotic cells at the heart part of the zebra fish are respectively 4621.9 pixels, 2075.7 pixels and 2973.57 pixels, compared with a model control group (6108.2 pixels), the improvement rates of the areas of thrombus are respectively 24%, 66% and 51%, and the traditional Chinese medicine mixed liquor with the concentrations of 1.74 mu L/mL, 5.21 mu L/mL and 15.6 mu L/mL has obvious improvement effect on the areas of thrombus in the zebra fish brain, wherein the improvement rates of the areas of thrombus are respectively 0.05, 0.05 and 0.001.

In conclusion, by scientifically proportioning the components of the traditional Chinese medicinal materials in the embodiment of the invention, the drug effects of the components can be matched with each other to generate a synergistic effect, and the traditional Chinese medicinal materials have the effects of nourishing blood, promoting blood circulation, removing blood stasis and relieving pain; the Chinese medicinal composition has the effects of improving myocardial apoptosis, reducing cardiac output and reducing blood flow rate, wherein the cardiac output increase rate can reach 93%, and the incidence rate and the area of cerebral thrombosis can be reduced.

The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A traditional Chinese medicine composition is characterized by comprising the following components in parts by weight: 10-50 parts of angelica sinensis, 10-50 parts of ligusticum wallichii, 10-50 parts of caulis spatholobi, 3-17 parts of rhizoma corydalis and 1-5 parts of pseudo-ginseng.

2. The traditional Chinese medicine composition according to claim 1, which comprises the following components in parts by weight: 25-40 parts of angelica, 25-40 parts of ligusticum wallichii, 25-40 parts of caulis spatholobi, 8-13 parts of rhizoma corydalis and 2.5-4 parts of pseudo-ginseng.

3. The traditional Chinese medicine composition according to claim 1, which comprises the following components in parts by weight: 30 parts of angelica, 30 parts of ligusticum wallichii, 30 parts of caulis spatholobi, 10 parts of rhizoma corydalis and 3 parts of pseudo-ginseng.

4. The Chinese medicinal composition of claim 1, further comprising adjuvants for preparing the Chinese medicinal composition into various pharmaceutical dosage forms.

5. The composition of claim 4, wherein the dosage form comprises a decoction, a granule, a tablet, a pill, a drink tablet or a capsule.

6. A method for preparing the Chinese medicinal composition as claimed in any one of claims 1 to 5, wherein the Chinese angelica root, Ligusticum wallichii, spatholobus stem and rhizoma corydalis are decocted with water, and then pseudo-ginseng is added and mixed to obtain the Chinese medicinal mixture.

7. The preparation method of the traditional Chinese medicine composition according to claim 6, wherein the angelica, the ligusticum wallichii, the caulis spatholobi and the rhizoma corydalis are added with 3-6 times of water, the mixture is heated until the water is boiled, the decoction is carried out for 10-30 min, the filtrate is obtained by filtration, and the pseudo-ginseng is added and mixed to obtain the traditional Chinese medicine mixed solution;

preferably, the pseudo-ginseng is pseudo-ginseng powder.

8. Use of the Chinese medicinal composition of any one of claims 1 to 5 in the preparation of a medicament for treating myocardial injury;

preferably, the myocardial injury comprises one or both of myocardial apoptosis and myocardial ischemia.

9. The use of the Chinese medicinal composition of any one of claims 1 to 5 in the preparation of a medicament for treating cerebrovascular diseases.

10. The use of claim 9, wherein the cerebrovascular disease comprises cerebral thrombosis.