CN113785920A - Probiotics bait for fry growth and preparation method thereof - Google Patents

Probiotics bait for fry growth and preparation method thereof Download PDF

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Publication number
CN113785920A
CN113785920A CN202111089093.6A CN202111089093A CN113785920A CN 113785920 A CN113785920 A CN 113785920A CN 202111089093 A CN202111089093 A CN 202111089093A CN 113785920 A CN113785920 A CN 113785920A
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parts
fermentation
probiotic
bait
mixing
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代作光
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Sichuan Hongguang Agricultural Development Co ltd
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Sichuan Hongguang Agricultural Development Co ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K50/00Feeding-stuffs specially adapted for particular animals
    • A23K50/80Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/10Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/20Animal feeding-stuffs from material of animal origin
    • A23K10/22Animal feeding-stuffs from material of animal origin from fish
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K10/00Animal feeding-stuffs
    • A23K10/30Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
    • A23K10/37Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
    • A23K10/38Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material from distillers' or brewers' waste
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/10Organic substances
    • A23K20/158Fatty acids; Fats; Products containing oils or fats
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K20/00Accessory food factors for animal feeding-stuffs
    • A23K20/20Inorganic substances, e.g. oligoelements
    • A23K20/24Compounds of alkaline earth metals, e.g. magnesium
    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23KFODDER
    • A23K40/00Shaping or working-up of animal feeding-stuffs
    • A23K40/10Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/80Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
    • Y02A40/81Aquaculture, e.g. of fish
    • Y02A40/818Alternative feeds for fish, e.g. in aquacultures
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87Re-use of by-products of food processing for fodder production

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Biotechnology (AREA)
  • Physiology (AREA)
  • Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Mycology (AREA)
  • Botany (AREA)
  • Marine Sciences & Fisheries (AREA)
  • Biomedical Technology (AREA)
  • Insects & Arthropods (AREA)
  • Birds (AREA)
  • Sustainable Development (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Inorganic Chemistry (AREA)
  • Feed For Specific Animals (AREA)
  • Fodder In General (AREA)

Abstract

The invention discloses a preparation method of a probiotic bait for fry growth, which comprises the following steps: step 10, selecting raw materials; step 20, soaking; step 30, cleaning; step 40, preparing filtered juice; step 50, weighing raw materials; step 60, mixing and fermenting for the first time; step 70, mixing and fermenting for the second time; and 80, drying and granulating. The probiotic bait prepared by the invention is beneficial to the growth and development of the fry, and can improve the immunity of the fry, thereby increasing the survival rate of the fry.

Description

Probiotics bait for fry growth and preparation method thereof
Technical Field
The invention belongs to the technical field of fishery breeding, and particularly relates to a probiotic bait for fry growth and a preparation method thereof.
Background
The lakes in China are numerous, the fishery resources are rich, and the freshwater fishes such as carps, crucian carps, silver carps, grass carps, black carps and the like are the main meat aquatic foods of people. Therefore, the scale of fish farming, particularly fresh water fish farming, is increasing. In the fish culture process, the particularly important stage is the growth and development stage of fish fries, and the stage is related to the subsequent growth health and growth speed of fish. In order to increase the survival rate of the fry, most of the current farmers feed antibiotics in the fry stage, and the antibiotics play roles in preventing diseases, improving feed coefficient and the like in the growth promotion process of fish culture, thereby improving the culture benefit. However, the long-term abuse of antibiotics brings about a plurality of mischief, such as bacterial drug resistance, superbacterial generation, DNA pollution and the like, influences the micro-ecological balance and the immunity of animals, directly influences the health of human bodies through food chain conduction, and increases the risk of human body injury. At present, people are prohibited to add antibiotics during the cultivation process in many countries, such as korea, japan, european union, etc., China also sets up relevant laws and regulations, and the world cultivation industry gradually moves into the "post-antibiotic" era.
Therefore, the development of new pollution-free antibiotic substitutes is urgent and becomes an urgent problem in the development of the feed industry. At present, a plurality of baits for replacing antibiotics exist in the market, but most of the baits have no obvious effect on the growth and development of the fry and the improvement of the immunity of the fry. Therefore, most of the baits on the market cannot meet the actual demand.
Disclosure of Invention
The technical problem to be solved by the invention is to provide a probiotic bait for fry growth and a preparation method thereof aiming at the defects of the prior art, and the prepared probiotic bait is beneficial to fry growth and development and can improve the immunity of the fry so as to increase the survival rate of the fry.
The technical scheme adopted by the invention is as follows: a preparation method of a probiotic bait for fry growth comprises the following steps:
step 10, selecting raw materials: selecting fresh and insect-free polygonum multiflorum stems, manually removing moulds and impurities for later use, and entering the step 20;
step 20, soaking: mixing water and salt at a weight ratio of 100:1-3, sufficiently mixing to obtain a soaking solution, soaking the caulis Polygoni Multiflori treated in step 10 in the soaking solution for 20-40 min, and entering step 30;
step 30, cleaning: filtering the polygonum multiflorum stems soaked in the step 20 by using a mesh screen to remove soaking liquid, washing the polygonum multiflorum stems by using pure water, drying in the air, cutting, crushing to obtain polygonum multiflorum powder, and entering the step 40;
step 40, preparing filtered juice: mixing 100 parts of water and 10 parts of polygonum multiflorum powder prepared in the step 30, boiling, evaporating 30 parts of water, filtering by using a 1200-mesh filter screen to obtain 80 parts of filtered juice, and entering the step 50;
step 50, weighing the following raw materials: weighing 100 parts of corn, wheat flour, bean pulp, oil cake, bean curd residue, vinasse, fish meal, soybean oil and calcium powder for later use, wherein the corn is 20-40 parts, the wheat flour is 20-40 parts, the bean pulp is 5-20 parts, the oil cake is 5-20 parts, the bean curd residue is 5-20 parts, the vinasse is 3-8 parts, the fish meal is 1-3 parts, the soybean oil is 1-3 parts and the calcium powder is 1-3 parts, and entering the step 60;
step 60, first mixed fermentation: feeding the raw materials weighed in the step 50 into a fermentation tank, mixing and stirring for 10-30 min, feeding 32 parts of the filtered juice prepared in the step 40 into the fermentation tank, uniformly mixing, adding 1-3 parts of fermentation probiotic powder, sealing and carrying out anaerobic fermentation for 7-10 days to obtain a first fermentation liquid, and then carrying out the step 70;
step 70, secondary mixed fermentation: feeding 48 parts of the filtered juice prepared in the step 40 into a fermentation tank, uniformly mixing, sealing, performing anaerobic fermentation for 15-30 days to obtain a fermented product, and performing the step 80;
step 80, drying and granulating: and (5) drying the fermentation product obtained in the step (80), then feeding the fermentation product into a granulator for granulation, and drying to obtain the probiotic bait.
In one embodiment, in step 20, the ratio of water to salt is 100:1, and the soaking time is 30 min.
In one embodiment, in the step 40, the boiling temperature is 100-120 ℃.
In one embodiment, in step 50, the raw materials include 30 parts of corn, 30 parts of wheat flour, 10 parts of soybean meal, 10 parts of oil cake, 10 parts of bean curd residue, 5 parts of vinasse, 2 parts of fish meal, 2 parts of soybean oil and 1 part of calcium powder.
In one embodiment, in step 60, the mixing and stirring time is 10min, 1 part of fermentation probiotic powder is added, and the sealed anaerobic fermentation time is 7 days.
In one embodiment, the sealed anaerobic fermentation time in step 70 is 15 days.
In one embodiment, in steps 60 and 70, the sealed anaerobic fermentation temperature is less than 70 ℃, and the fermented product is irregularly stirred and turned during the sealed anaerobic fermentation process.
The invention also discloses a probiotic bait for fry growth, which is prepared by the preparation method of the probiotic bait for fry growth.
The invention has the beneficial effects that:
1. decocting caulis Polygoni Multiflori to obtain filtrate, performing sealed anaerobic fermentation with various raw materials, and inoculating lactobacillus during preparation process to obtain fermented product containing abundant beneficial bacteria and elements, wherein the prepared probiotic bait is helpful for fry growth and development, and can improve immunity of fry, thereby increasing survival rate of fry;
2. the sealed anaerobic fermentation is divided into two stages, and the filtered juice obtained by decocting polygonum multiflorum stems is added twice, so that the sealed anaerobic fermentation effect is fully ensured.
Detailed Description
The present invention will be described in further detail with reference to specific examples.
Example 1:
the invention discloses a preparation method of a probiotic bait for fry growth, which comprises the following steps:
step 10, selecting raw materials: selecting fresh and insect-free polygonum multiflorum stems, manually removing moulds and impurities for later use, and entering the step 20;
step 20, soaking: mixing water and salt at a weight ratio of 100:1-3, sufficiently mixing to obtain a soaking solution, soaking the caulis Polygoni Multiflori treated in step 10 in the soaking solution for 20-40 min, and entering step 30;
step 30, cleaning: filtering the polygonum multiflorum stems soaked in the step 20 by using a mesh screen to remove soaking liquid, washing the polygonum multiflorum stems by using pure water, drying in the air, cutting, crushing to obtain polygonum multiflorum powder, and entering the step 40;
step 40, preparing filtered juice: mixing 100 parts of water and 10 parts of polygonum multiflorum powder prepared in the step 30, boiling, evaporating 30 parts of water, filtering by using a 1200-mesh filter screen to obtain 80 parts of filtered juice, and entering the step 50;
step 50, weighing the following raw materials: weighing 100 parts of corn, wheat flour, bean pulp, oil cake, bean curd residue, vinasse, fish meal, soybean oil and calcium powder for later use, wherein the corn is 20-40 parts, the wheat flour is 20-40 parts, the bean pulp is 5-20 parts, the oil cake is 5-20 parts, the bean curd residue is 5-20 parts, the vinasse is 3-8 parts, the fish meal is 1-3 parts, the soybean oil is 1-3 parts and the calcium powder is 1-3 parts, and entering the step 60;
step 60, first mixed fermentation: feeding the raw materials weighed in the step 50 into a fermentation tank, mixing and stirring for 10-30 min, feeding 32 parts of the filtered juice prepared in the step 40 into the fermentation tank, uniformly mixing, adding 1-3 parts of fermentation probiotic powder, sealing and carrying out anaerobic fermentation for 7-10 days to obtain a first fermentation liquid, and then carrying out the step 70;
step 70, secondary mixed fermentation: feeding 48 parts of the filtered juice prepared in the step 40 into a fermentation tank, uniformly mixing, sealing, performing anaerobic fermentation for 15-30 days to obtain a fermented product, and performing the step 80;
step 80, drying and granulating: and (5) drying the fermentation product obtained in the step (80), then feeding the fermentation product into a granulator for granulation, and drying to obtain the probiotic bait.
In the embodiment, the polygonum multiflorum thunb vine is boiled to obtain the filtered juice, and then is subjected to sealed anaerobic fermentation with various raw materials, and meanwhile, lactic acid bacteria are accessed in the preparation process, so that the fermentation product contains abundant beneficial bacteria and beneficial elements, the prepared probiotic bait is beneficial to the growth and development of the fry, the immunity of the fry can be improved, and the survival rate of the fry is increased. The sealed anaerobic fermentation is divided into two stages, and the filtered juice obtained by decocting polygonum multiflorum stems is added twice, so that the sealed anaerobic fermentation effect is fully ensured.
Example 2:
based on the above examples, in step 20, the ratio of water to salt is 100:1, and the soaking time is 30 min.
The saline mixture with the proportion can kill a part of bacteria on the tuber fleeceflower stem, and cannot damage the tuber fleeceflower stem.
Example 3:
on the basis of the above embodiment, in the step 40, the boiling temperature is 100-120 ℃.
The boiling temperature is set according to the setting, so that bacteria on the polygonum multiflorum rattan can be effectively killed through high temperature, and meanwhile, the polygonum multiflorum rattan cannot be damaged.
Example 4:
on the basis of the above embodiment, in step 50, the raw materials weighed include 30 parts of corn, 30 parts of wheat flour, 10 parts of soybean meal, 10 parts of oil cake, 10 parts of bean curd residue, 5 parts of distiller's grains, 2 parts of fish meal, 2 parts of soybean oil and 1 part of calcium powder.
The proportion fully meets the requirements of the fry on various beneficial elements in the growth process, and the proportion is suitable for various types of fry. In the actual use process, the requirements under most conditions can be met only by fine adjustment on the basis of the proportion according to the actual conditions.
Example 5:
on the basis of the above embodiment, in step 60, the mixing and stirring time is 10min, 1 part of fermented probiotic powder is added, and the sealed anaerobic fermentation time is 7 days.
The stirring time, the parts of the fermented probiotic powder and the sealed anaerobic fermentation time are selected according to actual conditions. However, the above parameters can basically meet the preparation requirements, save time and improve efficiency. Meanwhile, the compatibility degree between the added fermentation probiotic powder and the filtered juice and among various raw materials is higher.
Example 6:
in step 70, the sealed anaerobic fermentation time is 15 days based on the above examples.
Example 7:
on the basis of the above examples, in step 60 and step 70, the sealed anaerobic fermentation temperature is less than 70 ℃, and the fermented product is irregularly stirred and turned during the sealed anaerobic fermentation process.
The temperature of the sealed anaerobic fermentation is less than 70 ℃, so that the beneficial elements in the filtered juice, various raw materials and the fermented probiotic powder are not damaged in the process of the sealed anaerobic fermentation, and meanwhile, the best fermentation environment can be provided for various beneficial elements by irregularly stirring and turning the fermented product, so that the fermentation effect is ensured.
The invention also discloses a probiotic bait for fry growth, which is prepared by the preparation method of the probiotic bait for fry growth in any one of the embodiments 1-7.
The above-mentioned embodiments only express the specific embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention.

Claims (8)

1. A preparation method of a probiotic bait for fry growth is characterized by comprising the following steps:
step 10, selecting raw materials: selecting fresh and insect-free polygonum multiflorum stems, manually removing moulds and impurities for later use, and entering the step 20;
step 20, soaking: mixing water and salt at a weight ratio of 100:1-3, sufficiently mixing to obtain a soaking solution, soaking the caulis Polygoni Multiflori treated in step 10 in the soaking solution for 20-40 min, and entering step 30;
step 30, cleaning: filtering the polygonum multiflorum stems soaked in the step 20 by using a mesh screen to remove soaking liquid, washing the polygonum multiflorum stems by using pure water, drying in the air, cutting, crushing to obtain polygonum multiflorum powder, and entering the step 40;
step 40, preparing filtered juice: mixing 100 parts of water and 10 parts of polygonum multiflorum powder prepared in the step 30, boiling, evaporating 30 parts of water, filtering by using a 1200-mesh filter screen to obtain 80 parts of filtered juice, and entering the step 50;
step 50, weighing the following raw materials: weighing 100 parts of corn, wheat flour, bean pulp, oil cake, bean curd residue, vinasse, fish meal, soybean oil and calcium powder for later use, wherein the corn is 20-40 parts, the wheat flour is 20-40 parts, the bean pulp is 5-20 parts, the oil cake is 5-20 parts, the bean curd residue is 5-20 parts, the vinasse is 3-8 parts, the fish meal is 1-3 parts, the soybean oil is 1-3 parts and the calcium powder is 1-3 parts, and entering the step 60;
step 60, first mixed fermentation: feeding the raw materials weighed in the step 50 into a fermentation tank, mixing and stirring for 10-30 min, feeding 32 parts of the filtered juice prepared in the step 40 into the fermentation tank, uniformly mixing, adding 1-3 parts of fermentation probiotic powder, sealing and carrying out anaerobic fermentation for 7-10 days to obtain a first fermentation liquid, and then carrying out the step 70;
step 70, secondary mixed fermentation: feeding 48 parts of the filtered juice prepared in the step 40 into a fermentation tank, uniformly mixing, sealing, performing anaerobic fermentation for 15-30 days to obtain a fermented product, and performing the step 80;
step 80, drying and granulating: and (5) drying the fermentation product obtained in the step (80), then feeding the fermentation product into a granulator for granulation, and drying to obtain the probiotic bait.
2. The method of claim 1, wherein in step 20, the ratio of water to salt is 100:1 and the soaking time is 30 min.
3. The method for preparing a probiotic bait for fry growth according to claim 1 or 2, wherein the cooking temperature in the step 40 is 100-120 ℃.
4. The method for preparing a probiotic bait for fry growth according to claim 1 or 2, wherein in the step 50, the weighed raw materials comprise 30 parts of corn, 30 parts of wheat flour, 10 parts of bean pulp, 10 parts of oil cake, 10 parts of bean curd residue, 5 parts of vinasse, 2 parts of fish meal, 2 parts of soybean oil and 1 part of calcium powder.
5. The method for preparing a probiotic bait for fry growth according to claim 1 or 2, wherein in the step 60, the mixing and stirring time is 10min, 1 part of fermented probiotic powder is added, and the sealed anaerobic fermentation time is 7 days.
6. The method of claim 5, wherein the sealed anaerobic fermentation time in step 70 is 15 days.
7. The method of claim 6, wherein the sealed anaerobic fermentation temperature is less than 70 ℃ in steps 60 and 70, and the fermentation product is irregularly stirred and tumbled during the sealed anaerobic fermentation.
8. A probiotic bait for fry growth, characterized in that it is prepared by a method for preparing a probiotic bait for fry growth according to any one of claims 1 to 7.
CN202111089093.6A 2021-09-16 2021-09-16 Probiotics bait for fry growth and preparation method thereof Pending CN113785920A (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104872380A (en) * 2015-06-23 2015-09-02 浙江光碳农业发展有限公司 Antibiotic-free anaerobic fermented feed and preparation method thereof
CN106107216A (en) * 2016-06-17 2016-11-16 合肥丰瑞隆生物科技有限公司 Feed additive of Cyprinus carpio and preparation method thereof
CN107581399A (en) * 2017-08-15 2018-01-16 代作光 A kind of probiotics bait preparation method for fry growth
CN107594216A (en) * 2017-09-29 2018-01-19 界首市春风家庭农场 A kind of feed for improving carp fry resistance against diseases

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104872380A (en) * 2015-06-23 2015-09-02 浙江光碳农业发展有限公司 Antibiotic-free anaerobic fermented feed and preparation method thereof
CN106107216A (en) * 2016-06-17 2016-11-16 合肥丰瑞隆生物科技有限公司 Feed additive of Cyprinus carpio and preparation method thereof
CN107581399A (en) * 2017-08-15 2018-01-16 代作光 A kind of probiotics bait preparation method for fry growth
CN107594216A (en) * 2017-09-29 2018-01-19 界首市春风家庭农场 A kind of feed for improving carp fry resistance against diseases

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Application publication date: 20211214