CN113785072A - 启动子区域分析方法和用于实施该方法的细胞 - Google Patents

启动子区域分析方法和用于实施该方法的细胞 Download PDF

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Publication number
CN113785072A
CN113785072A CN202080025188.9A CN202080025188A CN113785072A CN 113785072 A CN113785072 A CN 113785072A CN 202080025188 A CN202080025188 A CN 202080025188A CN 113785072 A CN113785072 A CN 113785072A
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China
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cell
activity
promoter region
cells
carrier protein
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CN202080025188.9A
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English (en)
Chinese (zh)
Inventor
P.沙皮罗
V.查里
J.林-琼斯
J.拉默丁
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Eurydica Voex Products Co ltd
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Eurydica Voex Products Co ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
    • G01N2333/938—Hydrolases (3) acting on glycosyl compounds (3.2) acting on beta-galactose-glycoside bonds, e.g. beta-galactosidase

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Emergency Medicine (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CN202080025188.9A 2019-03-28 2020-03-26 启动子区域分析方法和用于实施该方法的细胞 Pending CN113785072A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962825559P 2019-03-28 2019-03-28
US62/825,559 2019-03-28
PCT/US2020/025055 WO2020198525A1 (en) 2019-03-28 2020-03-26 Promoter region analysis methods and cells for practicing same

Publications (1)

Publication Number Publication Date
CN113785072A true CN113785072A (zh) 2021-12-10

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CN202080025188.9A Pending CN113785072A (zh) 2019-03-28 2020-03-26 启动子区域分析方法和用于实施该方法的细胞

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US (1) US20200308591A1 (https=)
EP (1) EP3947714A4 (https=)
JP (1) JP2022528270A (https=)
KR (1) KR20210138766A (https=)
CN (1) CN113785072A (https=)
AU (1) AU2020248020A1 (https=)
CA (1) CA3116068A1 (https=)
IL (1) IL286671B2 (https=)
WO (1) WO2020198525A1 (https=)

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TW202328452A (zh) * 2021-09-07 2023-07-16 俄羅斯聯邦商拜奧卡德聯合股份公司 誘導型啟動子、基於其的載體和宿主細胞
CN115786340B (zh) * 2022-11-04 2023-12-05 江南大学 一种提高leaderless启动子活性的方法及其应用

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999002719A1 (en) * 1997-07-11 1999-01-21 The University Of British Columbia Complementation trap
WO2004070351A2 (en) * 2003-02-06 2004-08-19 Odyssey Thera, Inc. Protein fragment complementation assays for high-throughput and high-content screening
JP2015507200A (ja) * 2012-02-06 2015-03-05 ディスカヴァーエックス コーポレイション タンパク質存在量の測定による細胞内結合事象の検出
CN105659088A (zh) * 2013-06-26 2016-06-08 菲洛吉卡有限公司 用于监测肽的细胞运输的方法
CN108368535A (zh) * 2014-08-21 2018-08-03 欧陆迪斯卡沃爱克斯公司 用于测量配体与靶标蛋白质的结合和细胞接合的方法

Family Cites Families (7)

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Publication number Priority date Publication date Assignee Title
EP0338045B1 (en) * 1987-09-21 1994-08-24 Microgenics Corporation Solid-phase non-separation enzyme assay
US5223393A (en) * 1990-06-12 1993-06-29 Microgenics Corporation Detection of analytes having binding sites for at least two binding moieties
AU2217800A (en) * 1998-12-24 2000-07-31 Small Molecule Therapeutics, Inc. Methods and compositions for identifying protease modulators
WO2003065004A2 (en) * 2002-01-29 2003-08-07 Discoverx, Inc. Enzyme activation protease assay
JP4796059B2 (ja) * 2004-06-30 2011-10-19 ディスカヴァーエックス コーポレイション 細胞内修飾の分析
JP5303448B2 (ja) * 2006-03-13 2013-10-02 ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティ 親和性が減少した酵素の相補性レポーター系を用いた分子相互作用の検出
US8865421B2 (en) * 2008-07-02 2014-10-21 Discoverx Corporation Assays for nuclear hormone receptor binding

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1999002719A1 (en) * 1997-07-11 1999-01-21 The University Of British Columbia Complementation trap
WO2004070351A2 (en) * 2003-02-06 2004-08-19 Odyssey Thera, Inc. Protein fragment complementation assays for high-throughput and high-content screening
JP2015507200A (ja) * 2012-02-06 2015-03-05 ディスカヴァーエックス コーポレイション タンパク質存在量の測定による細胞内結合事象の検出
CN105659088A (zh) * 2013-06-26 2016-06-08 菲洛吉卡有限公司 用于监测肽的细胞运输的方法
CN108368535A (zh) * 2014-08-21 2018-08-03 欧陆迪斯卡沃爱克斯公司 用于测量配体与靶标蛋白质的结合和细胞接合的方法

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
KEITH R. OLSON等: "β Galactosidase Complementation: A Cell-Based Luminescent Assay Platform for Drug Discovery", ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES, vol. 5, no. 1, 13 March 2007 (2007-03-13), pages 137 - 144, XP055067075, DOI: 10.1089/adt.2006.052 *

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Publication number Publication date
WO2020198525A1 (en) 2020-10-01
KR20210138766A (ko) 2021-11-19
US20200308591A1 (en) 2020-10-01
JP2022528270A (ja) 2022-06-09
IL286671A (en) 2021-10-31
AU2020248020A1 (en) 2021-10-28
EP3947714A4 (en) 2022-12-14
IL286671B2 (en) 2024-01-01
IL286671B1 (en) 2023-09-01
EP3947714A1 (en) 2022-02-09
CA3116068A1 (en) 2020-10-01

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