CA3116068A1 - Promoter region analysis methods and cells for practicing same - Google Patents

Promoter region analysis methods and cells for practicing same Download PDF

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Publication number
CA3116068A1
CA3116068A1 CA3116068A CA3116068A CA3116068A1 CA 3116068 A1 CA3116068 A1 CA 3116068A1 CA 3116068 A CA3116068 A CA 3116068A CA 3116068 A CA3116068 A CA 3116068A CA 3116068 A1 CA3116068 A1 CA 3116068A1
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CA
Canada
Prior art keywords
cell
activity
promoter region
agent
carrier protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3116068A
Other languages
English (en)
French (fr)
Inventor
Paul Shapiro
Venkatesh Chari
Jennifer LIN-JONES
Jane Lamerdin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eurofins Discoverx Products LLC
Original Assignee
Eurofins Discoverx Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eurofins Discoverx Corp filed Critical Eurofins Discoverx Corp
Publication of CA3116068A1 publication Critical patent/CA3116068A1/en
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01023—Beta-galactosidase (3.2.1.23), i.e. exo-(1-->4)-beta-D-galactanase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/90—Enzymes; Proenzymes
    • G01N2333/914—Hydrolases (3)
    • G01N2333/924—Hydrolases (3) acting on glycosyl compounds (3.2)
    • G01N2333/938—Hydrolases (3) acting on glycosyl compounds (3.2) acting on beta-galactose-glycoside bonds, e.g. beta-galactosidase

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Emergency Medicine (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA3116068A 2019-03-28 2020-03-26 Promoter region analysis methods and cells for practicing same Pending CA3116068A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201962825559P 2019-03-28 2019-03-28
US62/825,559 2019-03-28
PCT/US2020/025055 WO2020198525A1 (en) 2019-03-28 2020-03-26 Promoter region analysis methods and cells for practicing same

Publications (1)

Publication Number Publication Date
CA3116068A1 true CA3116068A1 (en) 2020-10-01

Family

ID=72607352

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3116068A Pending CA3116068A1 (en) 2019-03-28 2020-03-26 Promoter region analysis methods and cells for practicing same

Country Status (9)

Country Link
US (1) US20200308591A1 (https=)
EP (1) EP3947714A4 (https=)
JP (1) JP2022528270A (https=)
KR (1) KR20210138766A (https=)
CN (1) CN113785072A (https=)
AU (1) AU2020248020A1 (https=)
CA (1) CA3116068A1 (https=)
IL (1) IL286671B2 (https=)
WO (1) WO2020198525A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TW202328452A (zh) * 2021-09-07 2023-07-16 俄羅斯聯邦商拜奧卡德聯合股份公司 誘導型啟動子、基於其的載體和宿主細胞
CN115786340B (zh) * 2022-11-04 2023-12-05 江南大学 一种提高leaderless启动子活性的方法及其应用

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0338045B1 (en) * 1987-09-21 1994-08-24 Microgenics Corporation Solid-phase non-separation enzyme assay
US5223393A (en) * 1990-06-12 1993-06-29 Microgenics Corporation Detection of analytes having binding sites for at least two binding moieties
US7062219B2 (en) * 1997-01-31 2006-06-13 Odyssey Thera Inc. Protein fragment complementation assays for high-throughput and high-content screening
CA2205888A1 (en) * 1997-07-11 1999-01-11 John J. Priatel Complementation trap
AU2217800A (en) * 1998-12-24 2000-07-31 Small Molecule Therapeutics, Inc. Methods and compositions for identifying protease modulators
WO2003065004A2 (en) * 2002-01-29 2003-08-07 Discoverx, Inc. Enzyme activation protease assay
JP4796059B2 (ja) * 2004-06-30 2011-10-19 ディスカヴァーエックス コーポレイション 細胞内修飾の分析
JP5303448B2 (ja) * 2006-03-13 2013-10-02 ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティ 親和性が減少した酵素の相補性レポーター系を用いた分子相互作用の検出
US8865421B2 (en) * 2008-07-02 2014-10-21 Discoverx Corporation Assays for nuclear hormone receptor binding
WO2013119579A1 (en) * 2012-02-06 2013-08-15 Discoveryx Corporation Detection of intracellular binding events by measuring protein abundance
JP2016523088A (ja) * 2013-06-26 2016-08-08 フィロジカ リミテッドＰｈｙｌｏｇｉｃａ Ｌｉｍｉｔｅｄ ペプチドの細胞輸送をモニタリングする方法
EP3183580B1 (en) * 2014-08-21 2025-03-26 Eurofins DiscoverX Corporation Homogenous thermal shift ligand binding assay

Also Published As

Publication number Publication date
WO2020198525A1 (en) 2020-10-01
KR20210138766A (ko) 2021-11-19
US20200308591A1 (en) 2020-10-01
JP2022528270A (ja) 2022-06-09
IL286671A (en) 2021-10-31
AU2020248020A1 (en) 2021-10-28
EP3947714A4 (en) 2022-12-14
IL286671B2 (en) 2024-01-01
CN113785072A (zh) 2021-12-10
IL286671B1 (en) 2023-09-01
EP3947714A1 (en) 2022-02-09

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