CN113785044A - 细胞操作装置及细胞操作方法 - Google Patents

细胞操作装置及细胞操作方法 Download PDF

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Publication number
CN113785044A
CN113785044A CN202080032753.4A CN202080032753A CN113785044A CN 113785044 A CN113785044 A CN 113785044A CN 202080032753 A CN202080032753 A CN 202080032753A CN 113785044 A CN113785044 A CN 113785044A
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cell
cells
cell manipulation
gas
liquid
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Chinese (zh)
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森山真树
川野武志
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Nikon Corp
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Nikon Corp
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/02—Devices for withdrawing samples
    • G01N1/10—Devices for withdrawing samples in the liquid or fluent state
    • G01N1/14—Suction devices, e.g. pumps; Ejector devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L99/00—Subject matter not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0068—General culture methods using substrates
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B01L2200/0668—Trapping microscopic beads
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0832—Geometry, shape and general structure cylindrical, tube shaped
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
    • C12N2509/10—Mechanical dissociation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Hydrology & Water Resources (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Sustainable Development (AREA)
  • Mechanical Engineering (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN202080032753.4A 2019-03-25 2020-03-25 细胞操作装置及细胞操作方法 Pending CN113785044A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2019-055986 2019-03-25
JP2019055986 2019-03-25
PCT/JP2020/013367 WO2020196635A1 (ja) 2019-03-25 2020-03-25 細胞操作デバイス及び細胞操作方法

Publications (1)

Publication Number Publication Date
CN113785044A true CN113785044A (zh) 2021-12-10

Family

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Family Applications (1)

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CN202080032753.4A Pending CN113785044A (zh) 2019-03-25 2020-03-25 细胞操作装置及细胞操作方法

Country Status (5)

Country Link
US (2) US12504353B2 (https=)
EP (1) EP3950920A4 (https=)
JP (3) JP7331921B2 (https=)
CN (1) CN113785044A (https=)
WO (1) WO2020196635A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP4219673A4 (en) * 2020-09-24 2025-05-07 Nikon Corporation METHOD FOR MANIPULATING AN ORGANISM AND DEVICE FOR MANIPULATING AN ORGANISM
CN116568800A (zh) * 2020-09-24 2023-08-08 株式会社尼康 生物体的操作方法及操作装置
EP4219677A4 (en) * 2020-09-24 2025-05-07 Nikon Corporation METHOD FOR EXERTING FORCE ON AN ORGANISM AND DEVICE FOR EXERTING FORCE ON AN ORGANISM
WO2022065459A1 (ja) * 2020-09-24 2022-03-31 株式会社ニコン 生物体操作方法および生物体操作装置

Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0350900U (https=) * 1989-09-21 1991-05-17
JP2010172231A (ja) * 2009-01-28 2010-08-12 Olympus Corp 細胞またはコロニーの採取装置及び細胞またはコロニーの採取方法
CN102971411A (zh) * 2010-07-01 2013-03-13 株式会社钟化 细胞培养用一次性器具、细胞培养装置及细胞制备方法
CN102985526A (zh) * 2010-07-16 2013-03-20 株式会社日立制作所 细胞培养容器及细胞培养装置
CN103068962A (zh) * 2010-08-12 2013-04-24 株式会社日立制作所 自动培养装置
WO2015098919A1 (ja) * 2013-12-27 2015-07-02 積水化学工業株式会社 細胞の剥離方法、細胞支持用基材、並びに、細胞の培養方法
US20180298318A1 (en) * 2015-12-30 2018-10-18 Berkeley Lights, Inc. Microfluidic Devices for Optically-Driven Convection and Displacement, Kits and Methods Thereof

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5549209B2 (ja) 2009-12-11 2014-07-16 株式会社Ｉｈｉ 付着性細胞培養装置
AU2014224784B2 (en) 2013-03-07 2018-03-08 F. Hoffmann-La Roche Ag Novel pyrazol derivatives
CN118562610A (zh) 2013-06-24 2024-08-30 威尔逊沃夫制造公司 用于透气性细胞培养过程的封闭系统装置和方法
JP6352895B2 (ja) 2015-12-25 2018-07-04 株式会社Ｓｃｒｅｅｎホールディングス 細胞剥離装置および細胞剥離方法

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH0350900U (https=) * 1989-09-21 1991-05-17
JP2010172231A (ja) * 2009-01-28 2010-08-12 Olympus Corp 細胞またはコロニーの採取装置及び細胞またはコロニーの採取方法
CN102971411A (zh) * 2010-07-01 2013-03-13 株式会社钟化 细胞培养用一次性器具、细胞培养装置及细胞制备方法
CN102985526A (zh) * 2010-07-16 2013-03-20 株式会社日立制作所 细胞培养容器及细胞培养装置
CN103068962A (zh) * 2010-08-12 2013-04-24 株式会社日立制作所 自动培养装置
WO2015098919A1 (ja) * 2013-12-27 2015-07-02 積水化学工業株式会社 細胞の剥離方法、細胞支持用基材、並びに、細胞の培養方法
US20180298318A1 (en) * 2015-12-30 2018-10-18 Berkeley Lights, Inc. Microfluidic Devices for Optically-Driven Convection and Displacement, Kits and Methods Thereof

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JP2023138839A (ja) 2023-10-02
JP2025074131A (ja) 2025-05-13
JPWO2020196635A1 (https=) 2020-10-01
US20260086001A1 (en) 2026-03-26
EP3950920A4 (en) 2023-02-01
EP3950920A1 (en) 2022-02-09
JP7331921B2 (ja) 2023-08-23
JP7643495B2 (ja) 2025-03-11
US20220170826A1 (en) 2022-06-02
WO2020196635A1 (ja) 2020-10-01
US12504353B2 (en) 2025-12-23

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