CN113768843B - A radix Sophorae Flavescentis extract for cosmetic and its preparation method - Google Patents
A radix Sophorae Flavescentis extract for cosmetic and its preparation method Download PDFInfo
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Abstract
The invention discloses a preparation method of a sophora flavescens extract for cosmetics, which is characterized by comprising the following steps of: step one, carrying out enzymolysis treatment on the sophora flavescens to obtain sophora flavescens zymolyte; step two, adding ethanol with the mass concentration of 70-80% into the sophora flavescens zymolyte, heating and extracting for 2-6h at 55-65 ℃, simultaneously decoloring, repeatedly extracting for 2-3 times, merging filtrate and filtering to obtain sophora flavescens crude extract; and step three, filtering the sophora flavescens crude extract to remove impurities, concentrating and removing a solvent to obtain the sophora flavescens extract. The method has simple process steps, combines enzymolysis and alcohol extraction, realizes the effect of extracting the sophora flavescens extract with high biological activity at a low temperature and a high yield, can effectively inhibit the growth of propionibacterium acnes, simultaneously inhibits inflammatory factors overexpressed due to the breeding of anaerobic bacteria acnes in skin, realizes the effect of treating both symptoms and root causes, and can be widely applied to cosmetics as an effective raw material.
Description
Technical Field
The invention relates to the technical field of cosmetic production, in particular to a sophora flavescens extract for cosmetics and a preparation method thereof.
Background
The radix sophorae flavescentis is the dried root of leguminous plant radix sophorae flavescentis (Sophoraflavicenes), is widely distributed in southern and northern provinces of China, is a common medicinal material of Guizhou Miao medicine, and has a long medicinal history. Recorded in Shen nong Ben Cao Jing, ku Shen is bitter and cold in flavor, and enters heart, liver, stomach, large intestine and bladder meridians. Governing heart and abdomen qi accumulation, abdominal mass accumulation, jaundice, drowning and dribbling, and expelling water. Remove abscess and swelling, tonify middle energizer, improve vision and relieve lacrimation. Modern large amount of pharmacological research finds that the radix sophorae flavescentis has pharmacological actions of resisting tumor, virus, bacteria and inflammation, resisting arrhythmia and the like, is more and more widely applied to treatment of diseases in oncology, hepatopathy, gynecology, dermatology, anorectal and cardiovascular departments, and has remarkable effect and high safety.
Acne is a common chronic inflammatory skin disease affecting pilosebaceous glands, the pathogenesis of which is not completely understood at present, but most scholars consider that the pathogenesis mainly comprises hyperseborrhea, hyperkeratosis and keratosis of follicular infundibulum and formation of keratotic plug, hyperproliferation of propionibacterium acnes and inflammatory reaction. Propionibacterium acnes was considered a critical component of pathogenesis, the earliest being the lipotropic intracellular parasitic bacilli isolated from lesions in acne patients, often parasitizing in the pilo-sebaceous gland infundibulum of human skin. A number of enzymes and actives produced by Propionibacterium acnes during metabolic activity have an injurious effect on skin cells and tissues, causing the follicular infundibulum wall barrier to be disrupted, triggering an acne inflammatory response. When immune cells of human body are acted by inflammatory factors, some soluble proteins or polypeptides with small molecular weight, information transmission among cells and specific immunoregulation function are secreted by the body and can participate or cause inflammatory reaction, and the substances are called inflammatory factors and comprise NO, TNF-alpha, IL-6, PGE-2, IL-1 and the like. Therefore, the anti-inflammatory and bacteriostatic work is well done, and the skin diseases such as acne, seborrheic dermatitis and the like can be effectively prevented and controlled.
The traditional Chinese medicine extraction method comprises a water decoction method, an immersion method, a percolation method, an alcohol extraction method and the like, and in order to improve the extraction rate, the prior art usually adopts higher extraction temperature for extraction. However, the higher extraction temperature easily causes the decomposition of the low melting point bioactive substance in Sophorae radix, thereby further improving its bioactivity.
Disclosure of Invention
In order to solve the above problems, the present invention aims to provide a method for preparing a sophora flavescens extract for cosmetics, which can effectively reduce the extraction temperature, increase the content of active ingredients, and further improve the anti-inflammatory and bacteriostatic activity, and comprises the following steps:
step one, carrying out enzymolysis treatment on the radix sophorae flavescentis to obtain radix sophorae flavescentis zymolyte;
step two, adding ethanol with the mass concentration of 70-80% into the sophora flavescens enzymatic hydrolysate, heating and extracting for 2-6h at 55-65 ℃, simultaneously decoloring, repeatedly extracting for 2-3 times, combining filtrates and filtering to obtain sophora flavescens crude extract;
and step three, filtering the sophora flavescens crude extract to remove impurities, concentrating and removing a solvent to obtain the sophora flavescens extract.
Further, in the first step, the step of enzymolysis specifically includes: adding water 2-3 times the weight of radix Sophorae Flavescentis, adding compound enzyme solution 3-5% of the weight of radix Sophorae Flavescentis, controlling pH to 4.5-6.5, and stirring at 40-45 deg.C for enzymolysis for 90-180min.
Further, the compound enzyme solution comprises the following components in an enzyme activity ratio of (3-9): (0.5-3): (0.5-2) xylanase, amylase, pectinase and protease (0.8-1.5), wherein the total enzyme activity in the compound enzyme solution is 10-12 ten thousand U/g.
Preferably, the enzyme activity ratio of the compound enzyme liquid is (4-6): (1-1.5): (0.8-1) and (1-1.2) xylanase, amylase, pectinase and protease.
Further, in the second step, 2-3 times of ethanol solution based on the weight of radix sophorae flavescentis is added into each 100g of the prepared zymolyte of radix sophorae flavescentis.
Preferably, in the second step, water bath heating is adopted.
Further, in the second step, activated carbon is used for decoloring.
Further, in the third step, the step of filtering and removing impurities comprises the step of carrying out vacuum filtration on the sophora flavescens crude extract by using a filter cake containing diatomite and EDTA-2Na, wherein the mass ratio of the diatomite to the EDTA-2Na is (2-5): (1-4).
Preferably, the vacuum filtration step is: mixing radix Sophorae Flavescentis crude extracts of different extraction times, cooling to about 40 deg.C, vacuum filtering, concentrating the filtrate under reduced pressure, recovering ethanol, concentrating the extractive solution to obtain extract, and weighing.
Further, the filter cake is prepared by the following method: placing diatomite and EDTA-2Na in water, stirring uniformly, vacuum filtering, and washing with water.
Preferably, the steps for preparing the diatomite filter cake are as follows: spreading the diatomite 5% + EDTA-2Na 1% in a beaker, uniformly stirring with pure water, vacuum-filtering to obtain a diatomite filter cake, eluting and washing the diatomite filter cake with 500mL of pure water, removing metal ions, and reducing the conductivity for later use.
Further, the method further comprises: mixing radix Sophorae Flavescentis extract with 1,3-propylene glycol, water and diatomite under stirring, standing overnight, filtering, dissolving the filtrate with 1,3-propylene glycol, sterilizing, and packaging.
On the other hand, the application also provides the sophora flavescens extract prepared by the preparation method.
On the other hand, the application also provides the application of the sophora flavescens extract prepared by the preparation method in preparing cosmetics, wherein the cosmetics comprise anti-inflammatory and bacteriostatic cosmetics.
Optionally, the anti-allergic and bacteriostatic cosmetic is in a dosage form including, but not limited to, aqua, lotion, cream, essence and gel.
The pretreatment of the plant complex enzyme can reduce the resistance from cell walls and intercellular substances during solvent extraction, accelerate the cell dissolution rate of effective components, improve the extraction efficiency and shorten the extraction time, and the enzyme method can act on a target product during traditional Chinese medicine extraction, improve the physicochemical property of the target product, improve the solubility of the target product in an extraction solvent, reduce the dosage of the solvent and reduce the cost. The 'bionic extraction' is a concept of extracting traditional Chinese medicine components proposed in recent years, in order to overcome the influence of traditional high-temperature decoction on the drug effect of active ingredients, the concept proposes that the extraction temperature is reduced to be close to the temperature of a human body, and the low-temperature extraction is not beneficial to the damage of plant medicine cell walls.
The invention has the following beneficial effects:
1. according to the preparation method of the sophora flavescens extract, the specific type and enzyme activity ratio of the complex enzyme are adopted for enzymolysis, and then the ethanol with specific concentration is adopted for extraction, so that active substances of alkaloids such as matrine in sophora flavescens can be extracted at low temperature, the extraction temperature is reduced, the content of bacteriostatic active ingredients in the extract is remarkably improved, and the anti-inflammatory and bacteriostatic activity of the sophora flavescens extract is further improved.
2. According to the preparation method of the sophora flavescens extract, the obtained product can effectively inhibit the growth of propionibacterium acnes, and can inhibit IL-1 beta, TNF-alpha and NO inflammatory factors overexpressed due to the breeding of anaerobic bacteria acnes in skin hair follicles, on one hand, both symptoms and root causes can be treated, on the other hand, the effects of inflammation resistance and bacteriostasis can be achieved, a foundation is laid for the application of the sophora flavescens extract in the field of cosmetics, and the sophora flavescens extract has the advantages of good naturality, NO toxic or side effect and the like.
Detailed Description
In the following description, numerous specific details are set forth by way of examples in order to provide a more thorough understanding of the present invention. It will be apparent, however, to one skilled in the art, that the present invention may be practiced without one or more of these specific details. In other instances, well-known features have not been described in order to avoid obscuring the invention.
The materials and equipment used in the following examples are commercially available, and if not specifically mentioned, the raw material grades in the following examples are all cosmetic grades and are all commercially available.
In addition, the "water" in the present invention includes any available water that can be used in the cosmetic field such as deionized water, distilled water, ion-exchanged water, double distilled water, high purity water, purified water, and the like.
Example 1
The embodiment provides a preparation method of a sophora flavescens extract for cosmetics, which comprises the following specific steps:
d1: weighing 500g of medicinal material radix sophorae flavescentis, adding water which is 3 times of the weight of the medicinal material radix sophorae flavescentis into the radix sophorae flavescentis, adding compound enzyme liquid which is 3% of the weight of the radix sophorae flavescentis, wherein the total enzyme activity in the compound enzyme liquid is about 10 ten thousand U/g, and the enzyme activity ratio is 5:1.2:0.9, 1.1, regulating the pH value to 5.0 by using oxalic acid, and stirring and performing enzymolysis at 40 ℃ for 100min to obtain a sophora flavescens zymolyte;
d2: extracting, decoloring and removing impurities, wherein the sophora flavescens zymolyte does not need to be filtered, the liquid and the residues are directly added with 60% ethanol which is 5 times of the weight of the sophora flavescens to be heated and extracted, the extraction temperature is 83 ℃, 80g of activated carbon is added for 1 hour of first extraction time, the mixture is uniformly stirred, heated in a water bath for 30min, then decoloring and hot filtering are carried out, 10g of activated carbon is added for 1 hour of second extraction, and after the mixture is uniformly stirred, heated in a water bath for 30min, decoloring and hot filtering are carried out, and the activated carbon and other impurities are removed, so that a clear and transparent solution is obtained;
d3: preparing a diatomite filter cake, paving (2% of diatomite + EDTA-2Na 4%) in a beaker, uniformly stirring with pure water, performing vacuum filtration to obtain the diatomite filter cake, eluting and washing the diatomite filter cake with 300mL of pure water, removing metal ions, and reducing the bottom conductivity for later use;
d4: vacuum filtering, cooling the two extractive solutions to 30 deg.C, vacuum filtering, mixing the two filtrates, concentrating under reduced pressure to recover ethanol, concentrating the extractive solution to obtain extract, and weighing;
d5: weighing radix Sophorae Flavescentis extract (alcoholic extract) 10%, adding 5% deionized water and 80% 1,3 propylene glycol, stirring for dissolving, adding 5% diatomite, stirring for 2 hr, standing overnight;
d6: carrying out vacuum filtration on the sample liquid, supplementing 1,3 propylene glycol to 50% of the total weight of the sample liquid after filtration, and stirring until the sample liquid is clear and transparent;
d7: heating and sterilizing at 70 deg.C for 0.5h, cooling to 20 deg.C, and packaging.
Example 2
The embodiment provides a preparation method of a sophora flavescens extract for cosmetics, which comprises the following specific steps:
d1: 500g of medicinal material radix sophorae flavescentis is weighed, water which is 3 times of the weight of the medicinal material radix sophorae flavescentis is added into the radix sophorae flavescentis, and a compound enzyme solution which is 3% of the weight of the radix sophorae flavescentis is added, wherein the total enzyme activity in the compound enzyme solution is about 10 ten thousand U/g, and the enzyme activity ratio is 5:1.2:0.9, 1.1, regulating the pH value to 5.0 by using oxalic acid, and stirring and performing enzymolysis at 40 ℃ for 100min to obtain a sophora flavescens zymolyte;
d2: extracting, decoloring and removing impurities, wherein the sophora flavescens zymolyte does not need to be filtered, the liquid and the residues are directly added with 70% ethanol which is 10 times of the weight of the sophora flavescens to be refluxed and extracted, the extraction temperature is 61 ℃, 60g of activated carbon is added into the first extraction time for 3h, the mixture is uniformly stirred and heated in a water bath for 60min, then decoloring and hot filtering are carried out, 30g of activated carbon is added into the second extraction time for 30 h, uniform stirring and heated in a water bath for 60min, then decoloring and hot filtering are carried out, and the activated carbon and other impurities are removed to obtain a clear and transparent solution;
d3: preparing a diatomite filter cake, paving (5% of diatomite + EDTA-2Na 1%) in a beaker, uniformly stirring with pure water, performing vacuum filtration to obtain the diatomite filter cake, eluting and washing the diatomite filter cake with 500mL of pure water, removing metal ions, and reducing the conductivity for later use;
d4: vacuum filtering, cooling the two extractive solutions to 40 deg.C, vacuum filtering, mixing the two filtrates, vacuum concentrating, recovering ethanol, concentrating the extractive solution to obtain extract, and weighing;
d5: weighing radix Sophorae Flavescentis extract (alcoholic extract) 5%, adding 25% deionized water and 70% 1,3 propylene glycol, stirring for dissolving, adding 10% diatomite, stirring for 2 hr, standing overnight;
d6: carrying out vacuum filtration on the sample liquid, supplementing 1,3 propylene glycol into the sample liquid after filtration till the total weight is 100%, and stirring till the sample liquid is clear and transparent;
d7: heating and sterilizing at 70 deg.C for 2 hr, cooling to 40 deg.C, and packaging.
Example 3
The embodiment provides a preparation method of a sophora flavescens extract for cosmetics, which comprises the following specific steps:
d1: weighing 500g of medicinal material radix sophorae flavescentis, adding water which is 3 times of the weight of the medicinal material radix sophorae flavescentis into the radix sophorae flavescentis, adding compound enzyme liquid which is 3% of the weight of the radix sophorae flavescentis, wherein the total enzyme activity in the compound enzyme liquid is about 10 ten thousand U/g, and the enzyme activity ratio is 5:1.2:0.9, 1.1, regulating the pH value to be 5.0 by using oxalic acid, and stirring and performing enzymolysis for 100min at 40 ℃ to obtain a sophora flavescens zymolyte;
d2: extracting, decoloring and removing impurities, wherein the sophora flavescens zymolyte does not need to be filtered, the liquid and the residues are directly added with 75% ethanol with the weight 15 times of that of the sophora flavescens for reflux extraction, the extraction temperature is 58 ℃, 70g of active carbon is added for 1.5h of first extraction time, the active carbon is stirred uniformly, heated in a water bath for 40min and then decolored and filtered while hot, 20g of active carbon is added for 2h of second extraction, the active carbon is stirred uniformly, heated in a water bath for 40min and then decolored and filtered while hot, and the active carbon and other impurities are removed to obtain a clear and transparent solution;
d3: preparing a diatomite filter cake, paving (diatomite 3% + EDTA-2Na 3%) in a beaker, uniformly stirring with pure water, performing vacuum filtration to obtain the diatomite filter cake, eluting and washing the diatomite filter cake with 400mL of pure water, removing metal ions, and reducing the conductivity for later use;
d4: vacuum filtering, cooling the two extractive solutions to 40 deg.C, vacuum filtering, mixing the two filtrates, concentrating under reduced pressure to recover ethanol, concentrating the extractive solution to obtain extract, and weighing;
d5: weighing radix Sophorae Flavescentis extract (alcohol extract) 15%, adding 10% deionized water and 65% 1,3 propylene glycol, stirring for dissolving, adding 10% diatomite, stirring for 2 hr, standing overnight;
d6: carrying out vacuum filtration on the sample liquid, supplementing 1,3 propylene glycol to 60% of the total weight of the sample liquid after filtration, and stirring until the sample liquid is clear and transparent;
d7: heating and sterilizing at 70 deg.C for 1h, cooling to 30 deg.C, and packaging.
Example 4
The embodiment provides a preparation method of a sophora flavescens extract for cosmetics, which comprises the following specific steps:
d1: weighing 500g of medicinal material radix sophorae flavescentis, adding water which is 3 times of the weight of the medicinal material radix sophorae flavescentis into the radix sophorae flavescentis, adding compound enzyme liquid which is 3% of the weight of the radix sophorae flavescentis, wherein the total enzyme activity in the compound enzyme liquid is about 10 ten thousand U/g, and the enzyme activity ratio is 5:1.2:0.9, 1.1, regulating the pH value to 5.0 by using oxalic acid, and stirring and performing enzymolysis at 40 ℃ for 100min to obtain a sophora flavescens zymolyte;
d2: extracting, decoloring and removing impurities, wherein the sophora flavescens zymolyte does not need to be filtered, the liquid and the residues are directly added with 80% ethanol which is 20 times of the weight of the sophora flavescens to be refluxed and extracted, the extraction temperature is 58 ℃, 50g of activated carbon is added for 3.5h of the first extraction time, the activated carbon is stirred uniformly, heated in a water bath for 50min and then decolored and filtered while hot, 30g of activated carbon is added for 1h of the second extraction, the water bath is stirred uniformly, heated for 50min and then decolored and filtered while hot, and the activated carbon and other impurities are removed to obtain a clear and transparent solution;
d3: preparing a diatomite filter cake, paving (diatomite 4% + EDTA-2Na 2%) in a beaker, uniformly stirring with pure water, performing vacuum filtration to obtain the diatomite filter cake, eluting and washing the diatomite filter cake with 600mL of pure water, removing metal ions, and reducing the conductivity for later use;
d4: vacuum filtering, cooling the two extractive solutions to 70 deg.C, vacuum filtering, mixing the two filtrates, concentrating under reduced pressure to recover ethanol, concentrating the extractive solution to obtain extract, and weighing;
d5: weighing radix Sophorae Flavescentis extract (alcoholic extract) 5%, adding 5% deionized water and 75% 1,3 propylene glycol, stirring for dissolving, adding 15% diatomaceous earth, stirring for 2 hr, standing overnight;
d6: carrying out vacuum filtration on the sample liquid, supplementing 1,3 propylene glycol into the sample liquid after filtration till the weight is 70 percent of the total weight, and stirring till the sample liquid is clear and transparent;
d7: heating and sterilizing at 100 deg.C for 3 hr, cooling to 20 deg.C, and packaging.
Example 5
The embodiment provides a preparation method of a sophora flavescens extract for cosmetics, which comprises the following specific steps:
d1: weighing 500g of medicinal material radix sophorae flavescentis, adding water which is 3 times of the weight of the medicinal material radix sophorae flavescentis into the radix sophorae flavescentis, adding compound enzyme liquid which is 3% of the weight of the radix sophorae flavescentis, wherein the total enzyme activity in the compound enzyme liquid is about 10 ten thousand U/g, and the enzyme activity ratio is 5:1.2:0.9, 1.1, regulating the pH value to 5.0 by using oxalic acid, and stirring and performing enzymolysis at 40 ℃ for 100min to obtain a sophora flavescens zymolyte;
d2: extracting, decoloring and removing impurities, wherein the sophora flavescens zymolyte does not need to be filtered, the liquid and the residues are directly added with 85% ethanol which is 15 times of the weight of the sophora flavescens to be refluxed and extracted, the extraction temperature is 42 ℃, 40g of active carbon is added for 4 hours of first extraction, the active carbon is uniformly stirred, the mixture is heated in 100 ℃ water bath for 70min and then is decolored and filtered while hot, 20g of active carbon is added for 2 hours of second extraction, the mixture is uniformly stirred, the mixture is heated in 100 ℃ water bath for 70min and then is decolored and filtered while hot, and the active carbon and other impurities are removed to obtain a clear and transparent solution;
d3: preparing a diatomite filter cake, paving (diatomite is 3% + EDTA-2Na 3%) in a beaker, uniformly stirring with pure water, performing vacuum filtration to obtain the diatomite filter cake, eluting and washing the diatomite filter cake with 350mL of pure water, removing metal ions, and reducing the bottom conductivity for later use;
d4: vacuum filtering, cooling the two extractive solutions to 80 deg.C, vacuum filtering, mixing the two filtrates, concentrating under reduced pressure to recover ethanol, concentrating the extractive solution to obtain extract, and weighing;
d5: weighing radix Sophorae Flavescentis extract (alcohol extract) 15%, adding 20% deionized water and 60% 1,3 propylene glycol, stirring for dissolving, adding 5% diatomaceous earth, stirring for 2 hr, standing overnight;
d6: carrying out vacuum filtration on the sample liquid, supplementing 1,3 propylene glycol into the sample liquid after the filtration till the weight is 80 percent of the total weight, and stirring till the sample liquid is clear and transparent;
d7: heating and sterilizing at 70 deg.C for 1h, cooling to 25 deg.C, and packaging.
Examples 6 to 8
Examples 6 to 8 were prepared in substantially the same manner as in example 2, except that the kind and ratio of the enzyme in the complex enzyme used in the first step, or the ethanol mass concentration in the second step were different, as shown in Table 1.
Comparative example 1
This comparative example is substantially the same as the preparation method of example 2, except that the first step is not employed, and the extraction of sophora flavescens is directly performed with ethanol.
The sophora flavescens extracts prepared in the above examples and comparative examples were tested for the minimum inhibitory concentration against propionibacterium acnes, and the specific test method is as follows. Meanwhile, the gram weight of the obtained extract is taken as an index, about 100g of extract (namely, about 20 percent of extraction rate) is obtained by controlling each embodiment, the lowest extraction temperature is obtained, and the obtained results are shown in table 1:
TABLE 1
As can be seen from the data in table 1, under the same condition that about 20% of extraction rate can be achieved, when the complex enzyme solution is used for enzymolysis and then is extracted with an ethanol aqueous solution, the extraction temperature can be reduced and the bacteriostatic activity can be significantly improved. In examples 2-4, the extraction temperature can be reduced to about 60 ℃, and the obtained sophora flavescens extract has high bacteriostatic active ingredients, and the minimum bacteriostatic concentration can reach 0.625%.
Further efficacy experiments were conducted using the sophora flavescens extract obtained in example 2 as the most preferred example.
1. Bacteriostatic test
1. Basis of test
The samples obtained in example 2 were subjected to bacteriostatic tests with reference to WS/T639-2018 technical requirements for antimicrobial susceptibility testing 4.2.1 agar dilution method.
2. Test method
2.1 sample preparation
a) The sample of example 2 was added to TSA autoclaved and at a temperature of 40 ℃ to 50 ℃ at concentrations of 0.625%, 0.125%, 0.025%, 3.125%, 6.25%, 12.5%, 25%, 50%, respectively.
b) After the agar and the sample are mixed well, pour the plate.
2.2 preparation of the bacterial suspension at a concentration of 1X 10 8 CFU/mL～2×10 8 CFU/mL, as initial inoculum. The initial inoculum was diluted 1.
2.3 sterile inoculating loop 1. Mu.L of bacterial suspension was applied to growth control plate without sample, TSA plate surface with sample (low to high concentration) and second growth control plate. The final inoculum size was 1X 104 CFU/spot.
2.4 placing in an anaerobic jar and culturing for 72h at 36 ℃.
3. Evaluation of bacteriostatic Effect
The plate was placed on a black, non-reflective background and the results were read. MIC was taken as the lowest concentration that inhibited macroscopic growth of bacteria. If the bacterial colony grows, the concentration sample has an antibacterial effect on the propionibacterium acnes, and if the bacterial colony grows, the concentration sample has no antibacterial effect on the propionibacterium acnes.
4. Test results
The growth of the flat lawn of the growth control group designed by the test is normal, which indicates that the test system is normal and meets the test standard. The results of colony growth inhibition of propionibacterium acnes by the samples at different concentrations are summarized as follows:
TABLE 2 results of colony growth inhibition of Propionibacterium acnes by samples at various concentration ranges
Based on propionibacterium acnes, the Minimum Inhibitory Concentration (MIC) of the sample radix sophorae flavescentis is 0.625% within the concentration range of 0.025% -0.625%, and the Minimum Inhibitory Concentration (MIC) is not detected within the concentration range of 3.125% -50%. The sophora flavescens extract prepared in the embodiment has obvious inhibition effect on the colony growth of propionibacterium acnes, and further has the effect of improving acne.
2. Experiment for inhibiting inflammatory factor
1. Principle of experiment
In the experiment, the anti-inflammatory effect of the sample No. 3 to be tested is evaluated by using RAW264.7 cells induced by LPS as an in-vitro inflammatory cell model and detecting inflammatory factors (IL-6, TNF-alpha and NO) secreted in cell supernatant through an ELISA kit.
2. The test steps are as follows:
2.1 cellular anti-inflammatory assays
2.1.1 culturing cells conventionally, inoculating the cells into a 12-well plate at a density of 2.0-3.0 × 105 cells/well, and returning to the incubator for culturing for 18-24h.
2.1.2 remove the plate, discard the original culture medium in the well, add 1mL of culture medium containing different concentrations of sample No. 3 and LPS (1. Mu.g/mL) to each well of the sample group, add culture medium with LPS (1. Mu.g/mL) as the stimulus to the model group, add 1mL of culture medium with dexamethasone (0.5. Mu.g/mL) and LPS (1. Mu.g/mL) to the positive control group, add culture medium to the control group, and culture for 24 + -1 h.
2.1.3 the supernatants were collected and stored at-80 ℃ and cytokines were measured using ELISA kits.
3. Data analysis
Data were analyzed using SPSS and expressed as mean ± standard deviation, and differences were considered statistically significant if p < 0.05.
3.1 cell Activity relative cell activity (viatility) was calculated for each group with the negative control (NT) cell activity as 100%.
3.2IL-6, TNF- α, NO levels based on 100% model control (M) relative to each group.
TABLE 3 comparison of IL-6 differences between different groups of samples
# denotes that the difference is statistically significant (p < 0.05)
* Shows that the difference is statistically significant (p < 0.05) compared to the model control group (M)
As can be seen from the data in Table 3, the IL-6 content in the negative control group (NT) was significantly reduced by 35.07% (P < 0.05) as compared to the model group (M); compared with the model group, the relative content of IL-6 in the positive control group (PC) is obviously reduced by 37.03 percent (P is less than 0.05), which indicates that the modeling is successful; under the concentration of 0.195% of the sophora flavescens extract in the sample group 3 (TA), the relative content of IL-6 is remarkably reduced by 29.42% (P < 0.05) compared with the model group.
TABLE 4 comparison of TNF-alpha differences between different groups of samples
# denotes that the difference is statistically significant (p < 0.05)
* Shows that the difference is statistically significant (p < 0.05)
As can be seen from the data in Table 4, the negative control group (NT) showed a significant decrease in TNF-. Alpha.content of 75.89% (P < 0.05) compared to the model group (M); compared with the model group, the relative content of TNF-alpha in the positive control group (PC) is obviously reduced by 77.77 percent (P is less than 0.05), which indicates that the modeling is successful; under the concentration of 0.106% of the sophora flavescens extract in the sample group 3 (TA), the relative content of TNF-alpha is remarkably reduced by 70.81% (P < 0.05) compared with the model group.
TABLE 5 comparison of NO differences between different groups of samples
# denotes that the difference is statistically significant (p < 0.05)
* Shows that the difference is statistically significant (p < 0.05)
As can be seen from the data in table 5, the negative control group (NT) showed a significant reduction in NO content of 78.64% (P < 0.05) compared to the model group (M); compared with the model group, the relative content of NO in the positive control group (PC) is obviously reduced by 44.48 percent (P is less than 0.05), which indicates that the molding is successful; at concentrations of "0.195%, 0.120% and 0.106% of the sophora flavescens extract of sample group 3 (TA), the relative content of NO was significantly reduced by 69.79%, 66.34% and 72.48% (P < 0.05), respectively, compared to the model group.
The sophora flavescens extract sample showed anti-inflammatory activity in this test. The problem of skin inflammation caused by the breeding of the anaerobic bacterium acnes can be prevented, cured or improved by inhibiting the three inflammation-promoting factors.
In summary, the plant extract provided by the application adopts the traditional Chinese medicine plant source, and has the advantages of good naturality, no toxic or side effect, mildness and no irritation. And laboratory tests show that enzymolysis and alcohol extraction are combined, so that the effect of extracting the sophora flavescens extract with high biological activity at a low temperature and a high yield is realized, the obtained product can effectively inhibit the growth of propionibacterium acnes, and meanwhile, IL-1 beta, TNF-alpha and NO inflammatory factors which are over-expressed due to the breeding of anaerobic bacteria acnes in skin hair follicles can be inhibited, so that on one hand, the effect of treating both symptoms and root causes can be realized, on the other hand, the anti-inflammatory and bacteriostatic effects can be realized, and a foundation is laid for the application of the anti-acne extract in the field of preparing acne inhibition medicines or anti-inflammatory skin care products.
The above description is only an example of the present application and is not intended to limit the present application. Various modifications and changes may occur to those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the scope of the claims of the present application.
Claims (8)
1. A preparation method of a sophora flavescens extract for cosmetics is characterized by comprising the following steps:
step one, carrying out enzymolysis treatment on the sophora flavescens to obtain sophora flavescens zymolyte;
step two, adding ethanol with the mass concentration of 70-80% into the sophora flavescens enzymatic hydrolysate, heating and extracting for 2-6h at 55-65 ℃, simultaneously decoloring, repeatedly extracting for 2-3 times, combining filtrates and filtering to obtain sophora flavescens crude extract;
step three, filtering the sophora flavescens crude extract to remove impurities, concentrating and removing a solvent to obtain the sophora flavescens extract;
in the first step, the step of enzymolysis specifically comprises: adding 2-3 times of water into radix Sophorae Flavescentis, adding 3-5% of compound enzyme solution, controlling pH to 4.5-6.5, and performing enzymolysis at 40-45 deg.C for 90-180min under stirring;
the compound enzyme liquid comprises the following components in an enzyme activity ratio of (3-9): (0.5-3): (0.5-2) xylanase, amylase, pectinase and protease (0.8-1.5), wherein the total enzyme activity in the compound enzyme solution is 10-12 ten thousand U/g.
2. The method of claim 1, wherein an ethanol solution is added to the hydrolysate of Sophora flavescens ait in an amount of 2 to 3 times the mass of Sophora flavescens ait per 100g of Sophora flavescens ait in the second step.
3. The method of preparing the sophora flavescens ait as claimed in claim 1, wherein the decoloring is performed by activated carbon in the second step.
4. The method for preparing the sophora flavescens ait extract as claimed in claim 1, wherein the third step of filtering to remove impurities comprises a step of vacuum filtration of sophora flavescens ait crude extract using a filter cake containing diatomaceous earth and EDTA-2Na, wherein the mass ratio of the diatomaceous earth to the EDTA-2Na is (2-5): (1-4).
5. The method of preparing the Sophorae radix extract for cosmetics according to claim 4, wherein the filter cake is prepared by: placing diatomite and EDTA-2Na in water, stirring uniformly, vacuum filtering, and washing with water.
6. The method of preparing the sophora flavescens ait extract for cosmetics according to claim 1, further comprising: mixing radix Sophorae Flavescentis extract with 1,3-propylene glycol, water and diatomite under stirring, standing overnight, filtering, dissolving the filtrate with 1,3-propylene glycol, sterilizing, and packaging.
7. The Sophora flavescens Aiton extract obtained by the process according to any one of claims 1 to 6.
8. The use of the Sophorae radix extract according to claim 7 for the preparation of a cosmetic, which comprises an anti-inflammatory and bacteriostatic cosmetic.
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