CN108078891B - Moisturizing and repairing facial cleanser containing periplaneta americana refined extract - Google Patents

Moisturizing and repairing facial cleanser containing periplaneta americana refined extract Download PDF

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CN108078891B
CN108078891B CN201711456343.9A CN201711456343A CN108078891B CN 108078891 B CN108078891 B CN 108078891B CN 201711456343 A CN201711456343 A CN 201711456343A CN 108078891 B CN108078891 B CN 108078891B
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periplaneta americana
water
extract
sodium
concentration
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CN108078891A (en
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郝振平
徐溧
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KUNMING SINOWAY NATURAL PHARMACEUTICALS CO Ltd
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KUNMING SINOWAY NATURAL PHARMACEUTICALS CO Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
    • A61K8/98—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin
    • A61K8/987—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution of animal origin of species other than mammals or birds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
    • A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/005—Antimicrobial preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
    • A61Q17/04—Topical preparations for affording protection against sunlight or other radiation; Topical sun tanning preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/08—Anti-ageing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A61Q19/10—Washing or bathing preparations
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
    • A61K2800/20—Chemical, physico-chemical or functional or structural properties of the composition as a whole
    • A61K2800/28—Rubbing or scrubbing compositions; Peeling or abrasive compositions; Containing exfoliants

Abstract

The invention relates to a moisturizing and repairing facial cleanser containing periplaneta americana extract. The components are as follows: 0.5-3% of periplaneta americana refined extract, 1-5% of prinsepia utilis royle oil, and acetylated lanolin alcohol: triterpene alcohol =3:2.5, 1-5% of the mixture obtained by mixing, 2-10% of alkyl glycoside, 2-7% of fatty alcohol polyoxyethylene ether, 1-6% of fatty alcohol polyoxyethylene ether sodium sulfate, 3-10% of glycerol, and sorbitol: 3-10% of 1, 2-pentanediol =7:4, 10-20% of sodium N-acylamidoate, and EDTA-Na41-3% of dosage, DMDM hydantoin: propylparaben =1:2 the resulting mixture is mixed 0.2-1% with the balance being water. The facial cleanser has good skin permeability, and has effects of promoting repair of damaged cells, promoting growth of new cells, improving cutin degeneration, improving crinkle circulation of human body, moistening facial skin, preventing chapping, and supplementing nutrition.

Description

Moisturizing and repairing facial cleanser containing periplaneta americana refined extract
Technical Field
The invention relates to the technical field of daily chemical products, and in particular relates to a moisturizing and repairing facial cleanser containing periplaneta americana extract.
Background
Because the human face is directly exposed in the air every day, the human face is not protected, and is directly contacted with the air for a long time, and the skin of the human face is easily dried or damaged due to the stimulation of wind blowing, sun drying, cold frost, a large amount of contact with cosmetics for a long time and the like. The dry or damaged facial skin can cause the skin to lose luster and softness, has no elasticity, is easy to wrinkle, prevents and treats dry skin manipulation, repairs the damaged facial skin, maintains the normal water content of the skin cuticle and repairs the damaged facial skin every day, and is very important for moistening and health of the facial skin. Along with the development of society, the washing and caring product for the facial skin is more and more valued, and the invention of the facial cleanser is particularly popular with modern young people and has better market prospect. At present, active ingredients with skin nourishing efficacy, such as bioactive components of various natural animal and plant extracts, are added into a formula of the facial cleanser, so that the facial cleanser provides nutrient ingredients for skin while cleaning the skin, and has a skin care function.
Periplaneta americana is an insect of the genus Periplaneta of the family Periplaneta of the order Periplaneta of the class Insecta, commonly called Blatta seu periplaneta, the largest volume insect of the family Blattadae, native to south America. Periplaneta americana is a traditional Chinese medicinal material, and is recorded in Shen nong's herbal Jing, which is called salty and cold in taste. Mainly has the effects of blood stasis, hard mass, cold and heat, mass breaking, throat and throat contraction, internal cold and no seed, and is used for treating infantile malnutrition, furuncle, carbuncle, tonsillitis, innominate toxic swelling, syphilis, venomous snake bite, centipede bite and the like.
Modern researches find that the periplaneta americana dry worm extract contains active substances such as WHF (polyhydric alcohols, epidermal growth factor), muconic acid, mucoglycine and various amino acids, and has the effects of resisting inflammation, reducing swelling, promoting cell proliferation and growth of new granulation tissues, accelerating repair of damaged tissues, accelerating shedding of necrotic tissues, improving the immune function of organisms and the like. The research also finds that the ethanol extract of the periplaneta americana contains a large amount of free amino acids, and contains 18 kinds of amino acids essential to human bodies and also contains a plurality of other amino acids, and the total number of the amino acids is up to 26. It is the combination of these specific amino acids that makes the periplaneta americana extract have the remarkable effect of promoting the repair of damaged cells and the growth of new cells.
Prinsepia utilis Royle, also called Prinsepia utilis Royle, is called Prinsepia utilis Royle in Yunnan herbal medicine, and is produced on hillsides and wasteland with the altitude of 1400-3100 m in Yunnan, Guizhou and Sichuan. The average oil yield of the prinsepia utilis royle is 28%, the fruit oil is rich in various nutrient components required by a human body, is high-grade natural edible oil, and has important auxiliary effects on treating hyperlipidemia, reducing cholesterol, preventing and treating cardiovascular diseases and the like. The fatty acid composition of the prinsepia utilis royle oil mainly comprises unsaturated fatty acids, namely oleic acid and linoleic acid, wherein the content of the oleic acid is 33.38%, and the content of the linoleic acid is 40.82%; in addition, the olive oil also contains 17.2% of palmitic acid and 6% of stearic acid, contains various vitamins such as vitamin A, vitamin D, vitamin E, vitamin K and the like, contains 3.5mg of vitamin A, 2.1mg of vitamin D, 11.2mg of vitamin E and 0.30mg of vitamin K per 100g of prinsepia utilis royle oil, also contains very rich major and trace elements such as calcium, sulfur, manganese, magnesium, potassium, phosphorus, zinc, iron and the like, and compared with other edible oils, the element with the highest content is potassium, and the content is 9.8 mg/kg.
The Prinsepia utilis Royle oil retains pure natural bioactive components, contains multiple fat-soluble vitamins and microelements which are easy to be absorbed by human body, a large amount of amino acids which are necessary for human body, and a fatty acid structure (1: 1: 0.8) which is very close to the proportion (1: 1: 1) of human body lipid, so that the Prinsepia utilis Royle oil has good permeability and is easy to be absorbed by skin, can keep the nutrition, moisture and elasticity of the skin, improve the degeneration of the horny layer of the skin and repair rough skin. Scientific research finds that the prinsepia utilis royle oil has no toxic or side effect on human bodies no matter the prinsepia utilis royle oil is taken orally or used externally; has no irritation to human skin; the skin has good permeability, can improve cutin degradation, and has effects of keeping moisture, preventing chapping, and supplementing nutrition; has obvious functions of diminishing inflammation, inhibiting bacteria, resisting ultraviolet ray, resisting senility, improving the circulation of crinkles and promoting metabolism.
The Chinese patent with the patent number 201210446628.5, which was filed by the inventor 2012, discloses a preparation method of a periplaneta americana extract for treating hepatitis b, and the extraction process is as follows:
A. inactivation and crushing: killing the fresh American cockroach by hot water at 80-90 ℃, controlling the temperature within 60 +/-5 ℃ for drying, crushing, and sieving by a 24-mesh sieve to prepare coarse American cockroach powder;
B. extraction: c, performing 3-5 times of reflux extraction on the periplaneta americana coarse powder obtained in the step A at the temperature of 75-80 ℃, and adding 90-95% ethanol in an amount which is 5-8 times the weight of the coarse powder during the first extraction for 4-6 hours; adding 90-95% ethanol in an amount which is 3-6 times the weight of the coarse powder every time after the second time, wherein the extraction time is 3-5 hours; in the extraction process, the temperature of ethanol is kept at 75-80 ℃, after extraction is finished, the extracting solutions are combined and filtered to obtain an ethanol extracting solution;
C. primary concentration: concentrating the ethanol extract obtained in the step B under reduced pressure under the conditions of vacuum degree of-0.05 to-0.06 MPa and temperature of 50 to 60 ℃ until the specific gravity is 1.05 to 1.15 to obtain an ethanol concentrated solution;
D. degreasing and adsorbing: adding distilled water which is 7-10 times of the weight of the ethanol concentrated solution into the ethanol concentrated solution obtained in the step C, heating to 80-90 ℃ while stirring, stirring for 10-60 minutes at a stirring speed of 20-60 revolutions per minute, and then keeping the temperature and standing for 1-5 hours to separate oil from water; collecting the lower-layer water solution, filtering the lower-layer water solution by using filter paper, adding medicinal active carbon which is 0.5 to 1.0 time of the ethanol concentrated solution into the obtained filtrate, and stirring the mixture at the temperature of between 40 and 60 ℃ for 30 to 60 minutes to ensure that the mixture is fully adsorbed;
E. and (3) elution: d, filling the active carbon-containing liquid medicine obtained in the step D into a chromatographic column, discharging the liquid medicine, washing with distilled water which is 10-30 times of the amount of the active carbon, and discarding the water washing liquid; eluting with 30-60 times of eluant of medicinal active carbon, collecting eluent, and filtering with filter paper to obtain elution filtrate;
F. secondary concentration: concentrating the elution filtrate obtained in the step E under reduced pressure at the temperature of 50-60 ℃ and the vacuum degree of-0.05-0.06 MPa until the specific gravity is 1.20-1.30 to obtain the periplaneta americana extract for treating hepatitis B;
G. drying and crushing: and F, drying the American cockroach extract for treating the hepatitis B in vacuum at the temperature of 50-60 ℃ and under the vacuum degree of-0.04-0.07 MPa, and crushing into fine powder to obtain the finished product of the American cockroach extract for treating the hepatitis B.
The patent is a patent granted to the inventor. The step E procedure of this patent application is elution: and D, filling the active carbon-containing liquid medicine obtained in the step D into a chromatographic column, discharging the liquid medicine, washing with distilled water which is 10-30 times of the amount of the active carbon, and discarding the water washing liquid. Through years of research and experiments of the inventor, the discarded liquid medicine and water washing liquid still contain a lot of valuable effective components of the periplaneta americana, and the discarded white cockroach is very unfortunately, so that how to fully utilize the effective components in the discarded liquid medicine and the water washing liquid becomes another research and development target of the inventor.
Disclosure of Invention
The invention aims to provide a moisturizing and repairing facial cleanser containing periplaneta americana extract, which is developed by using effective components in liquid medicine and washing liquid discarded in the step E in the preparation method of the periplaneta americana extract for treating hepatitis B disclosed by the Chinese patent with the patent number of 201210446628.5, has good skin permeability, can promote the repair of damaged cells, promote the growth of new cells, improve the degradation of cutin, diminish inflammation, inhibit bacteria, resist ultraviolet rays and aging, improve the circulation of crinkles of human bodies, and has the effects of moisturizing, cracking prevention and nutrition supplement on facial skin.
The present invention achieves the above object by the following method.
A moisturizing and repairing facial cleanser containing periplaneta americana refined extract comprises the following components in percentage by weight:
0.5-3% of periplaneta americana refined extract, 1-5% of prinsepia utilis royle oil, and acetylated lanolin alcohol: triterpene alcohol =3:2.5 mixture 1-5%, alkyl glycoside 2-10%, fatty alcohol polyoxypropylene ether 2-7%, fatty alcohol polyoxyethylene ether sodium sulfate 1-6%, glycerol 3-10%, sorbitol 1, 2-pentanediol =7:4 mixture 3-10%, sodium N-acyl amino acid 10-20%, EDTA-Na41-3% of dosage, DMDM hydantoin: propyl hydroxybenzoate =1:2, 0.2-1% of the mixture, 0.1-0.5% of essence, and the balance water.
Preferably, the moisturizing and repairing facial cleanser containing the periplaneta americana refined extract comprises the following components in percentage by weight:
0.9-2% of periplaneta americana refined extract, 1.8-3% of prinsepia utilis royle oil, and acetylated lanolin alcohol: triterpene alcohol =3:2.5 mixed mixture 2.5-4%, alkyl glycoside 6-9%, fatty alcohol polyoxypropylene ether 2-4%, fatty alcohol polyoxyethylene ether sodium sulfate 3.5-4.5%, glycerin 6-9%, sorbitol 1, 2-pentanediol =7:4 mixed mixture 7%, sodium N-acyl amino acid 15-18%, EDTA-Na41.5-2.5% of dosage, DMDM hydantoin: propyl hydroxybenzoate =1:2, 0.4-0.8% of the mixture obtained by mixing, 0.2-0.3% of essence and the balance of water.
Preferably, the essence is one or a mixture of more of green tea essence, cucumber essence and aloe essence.
The moisturizing and repairing facial cleanser containing the periplaneta americana refined extract is prepared by the following process:
s1, weighing the components for producing the facial cleanser according to the parts by weight;
s2, taking prinsepia utilis royle oil and acetylated lanolin alcohol: triterpene alcohol =3:2.5 mixing the obtained mixture, alkyl glycoside, fatty alcohol polyoxypropylene ether and fatty alcohol polyoxyethylene ether sodium sulfate, placing the mixture in an oil phase pot, stirring and heating to 40-60 ℃, and uniformly obtaining an oil phase A;
s3, putting water into a water phase pot, adding glycerol, sorbitol 1, 2-pentanediol =7:4, mixing the obtained mixture, sodium N-acyl amino acid and EDTA-Na4Stirring and heating to 60-70 ℃, and uniformly stirring to obtain a water phase B;
s4, uniformly mixing the oil phase A and the water phase B to obtain a material C;
s5, cooling the material C to 30-40 ℃, and adding the periplaneta americana refined extract and DMDM hydantoin in proportion: and (3) mixing the obtained mixture and the essence at a ratio of propyl hydroxybenzoate =1:2, and uniformly stirring at a high speed for 5-20 minutes to obtain the moisturizing and repairing facial cleanser containing the periplaneta americana extract.
Preferably, the periplaneta americana essence extract-containing moisturizing and repairing facial cleanser is prepared by refining the liquid medicine and the water washing liquid discarded in the step E in the preparation process of the periplaneta americana extract disclosed in the Chinese patent 201210446628.5 according to the following processes:
a. inactivation and crushing: killing the fresh American cockroach by hot water at 80-90 ℃, controlling the temperature within 60 +/-5 ℃ for drying, crushing, and sieving by a 24-mesh sieve to prepare coarse American cockroach powder;
b. extraction: c, performing 3-5 times of reflux extraction on the periplaneta americana coarse powder obtained in the step a at the temperature of 75-80 ℃, and adding 90-95% ethanol in an amount which is 5-8 times the weight of the coarse powder during the first extraction for 4-6 hours; adding 90-95% ethanol in an amount which is 3-6 times the weight of the coarse powder every time after the second time, wherein the extraction time is 3-5 hours; in the extraction process, the temperature of ethanol is kept at 75-80 ℃, after extraction is finished, the extracting solutions are combined and filtered to obtain an ethanol extracting solution;
c. primary concentration: c, concentrating the ethanol extract obtained in the step b under reduced pressure under the conditions of vacuum degree of-0.05 to-0.06 MPa and temperature of 50 to 60 ℃ until the specific gravity is 1.05 to 1.15 to obtain an ethanol concentrated solution;
d. degreasing and adsorbing: adding distilled water which is 7-10 times of the weight of the ethanol concentrated solution into the ethanol concentrated solution obtained in the step c, heating to 80-90 ℃ while stirring, stirring for 10-60 minutes at a stirring speed of 20-60 revolutions per minute, and then keeping the temperature and standing for 1-5 hours to separate oil from water; collecting the lower-layer water solution, filtering the lower-layer water solution by using filter paper, adding medicinal active carbon which is 0.5 to 1.0 time of the ethanol concentrated solution into the obtained filtrate, and stirring the mixture at the temperature of between 40 and 60 ℃ for 30 to 60 minutes to ensure that the mixture is fully adsorbed;
e. and (3) elution: d, filling the activated carbon-containing liquid medicine obtained in the step d into a chromatographic column, discharging the liquid medicine, washing with distilled water 10-30 times of the amount of the medicinal activated carbon, and collecting and uniformly mixing the liquid medicine and the washing liquid;
f. membrane separation: under the pressure of 50-200kPa, collecting the liquid medicine and the water washing liquid which are uniformly mixed in the step e, passing through an ultrafiltration ceramic membrane with the molecular cut-off value of 50kD, discarding the cut-off liquid, and collecting penetrating fluid;
g. secondary concentration: c, concentrating the penetrating fluid obtained in the step a under reduced pressure at the temperature of 50-60 ℃ and the vacuum degree of-0.05-0.06 MPa to 1/10-1/30 of the original volume;
h. ion exchange: taking the secondary concentrated solution obtained in the step g, using hydrochloric acid with the concentration of 1mol/L and sodium hydroxide with the concentration of 1mol/L to adjust the pH value of the concentrated penetrating fluid obtained in the step g to be 5-7, then passing through a regenerated sodium type HD-8 strong-acid styrene type cation exchange resin column, using sodium type HD-8 strong-acid styrene type cation exchange resin which is 3-7 times of the concentrated penetrating fluid obtained in the step g according to the volume, after the adsorption of the sodium type HD-8 strong-acid styrene type cation exchange resin column is finished, firstly using deionized water with the volume of 2-4 times of the amount of the sodium type HD-8 strong-acid styrene type cation exchange resin, then using ammonia water solution with the concentration of 1-3mol/L to elute, wherein the using amount of the ammonia water solution with the concentration of 1-3mol/L is 5-8 times of the amount of the sodium type HD-8 strong-acid styrene type cation exchange resin, controlling the flow rate of the eluent to ensure that the time of the whole elution process is 8-12 hours, and collecting the eluent;
i. and (3) third-stage concentration: and (5) concentrating the eluent obtained in the step h under reduced pressure at the temperature of 50-60 ℃ and the vacuum degree of-0.05-0.06 MPa to obtain an extract with the specific gravity of 1.20-1.30, thus obtaining the periplaneta americana refined extract.
Preferably, the process of the step f is membrane separation: under the pressure of 80kPa, collecting the liquid medicine and the water washing liquid which are uniformly mixed in the step e, passing through an ultrafiltration ceramic membrane with the molecular cut-off value of 50kD, discarding the cut-off liquid, and collecting penetrating fluid;
preferably, the process of the step g comprises two-stage concentration: and f, concentrating the penetrating fluid obtained in the step f under reduced pressure at the temperature of 50-60 ℃ and the vacuum degree of-0.05-0.06 MPa to 1/20 of the original volume.
Preferably, the process of the step h is that ion exchange: taking the secondary concentrated solution obtained in the step g, regulating the pH value of the concentrated penetrating fluid obtained in the step g to be 6.0 by using hydrochloric acid with the concentration of 1mol/L and sodium hydroxide with the concentration of 1mol/L, then passing through a regenerated sodium type HD-8 strong-acid styrene type cation exchange resin column, washing the sodium type HD-8 strong-acid styrene type cation exchange resin with deionized water with the volume of 3 times of the amount of the sodium type HD-8 strong-acid styrene type cation exchange resin after the adsorption of the sodium type HD-8 strong-acid styrene type cation exchange resin column is finished, then eluting by using ammonia water with the concentration of 1-3mol/L, wherein the using amount of the ammonia water with the concentration of 1.5mol/L is 6 times of the amount of the sodium type HD-8 strong-acid styrene type cation exchange resin, controlling the flow rate of the eluent to ensure that the time of the whole elution process is 10 hours, and collecting the eluent.
The invention has the beneficial effects that:
1. the moisturizing and repairing facial cleanser containing the periplaneta americana essence extract has good skin permeability, can deeply clean the skin, promote the repair of damaged cells, promote the growth of new cells, improve the degeneration of cutin, diminish inflammation, inhibit bacteria, resist ultraviolet rays and aging, improve the circulation of crinkles of human bodies, have the effects of locking water, moisturizing, preventing cracking and supplementing nutrition on the facial skin and can better improve the elasticity of the facial skin.
2. The periplaneta americana refined extract added in the invention is prepared by extracting the periplaneta americana extract for treating hepatitis B from the periplaneta americana and then refining the periplaneta americana extract by using waste liquid medicine and washing liquid, belongs to waste recycling, and can furthest utilize active substances in the periplaneta americana medicinal material, thereby achieving the purposes of recycling waste, reducing cost, increasing resource utilization rate, increasing profits, saving energy and reducing emission, and effectively improving the product competitiveness of enterprises.
3. The American cockroach refined extract contains up to 26 pure natural small molecular amino acids, wherein the amino acids contain 18 amino acids necessary for human bodies, and by the process, the ineffective components are removed, so that the total amino acid content in the refined extract is up to 60-80%, and the high-quality American cockroach refined extract ensures that the facial cleanser has a better facial cleaning effect.
4. The periplaneta americana extract extracted by the process can effectively remove peculiar smell and color of the periplaneta americana, the produced periplaneta americana extract is almost colorless and tasteless, and is added as a raw material for producing facial cleanser, the color of the facial cleanser produced is not influenced by the color of the periplaneta americana and is free of peculiar smell of the periplaneta americana, and consumers can accept the periplaneta americana extract easily.
Detailed Description
The present invention is further illustrated by the following specific examples, which are provided for illustrative purposes only and do not limit the scope of the present invention.
Example 1
The facial cleanser containing the periplaneta americana refined extract for moisturizing and repairing consists of the following components in percentage by weight: 1% of periplaneta americana refined extract, 2% of prinsepia utilis royle oil, and acetylated lanolin alcohol: triterpene alcohol =3:2.5 mixture 3%, alkyl glycoside 8%, fatty alcohol polyoxypropylene ether 2.5%, sodium fatty alcohol polyoxyethylene ether sulfate 4%, glycerin 7%, sorbitol 1, 2-pentanediol =7:4 mixture 7%, sodium N-acyl amino acid 17%, EDTA-Na4Amount 2%, DMDM hydantoin: propylparaben =1:2 the resulting mixture was mixed 0.6%, green tea flavor 0.2%, and water balance.
The moisturizing and repairing facial cleanser containing the periplaneta americana refined extract is prepared by the following process:
s1, weighing the components for producing the facial cleanser according to the parts by weight;
s2, taking prinsepia utilis royle oil and acetylated lanolin alcohol: triterpene alcohol =3:2.5 mixing the obtained mixture, alkyl glycoside, fatty alcohol polyoxypropylene ether and fatty alcohol polyoxyethylene ether sodium sulfate, placing the mixture in an oil phase pot, stirring and heating to 40 ℃, and uniformly obtaining an oil phase A;
s3, putting water into a water phase pot, adding glycerol and mountain1, 2-pentanediol =7:4, sodium N-acylamidoate, EDTA-Na4Stirring and heating to 60 ℃, and uniformly stirring to obtain a water phase B;
s4, uniformly mixing the oil phase A and the water phase B to obtain a material C;
s5, cooling the material C to 30 ℃, and adding the periplaneta americana refined extract and the DMDM hydantoin in proportion: and (3) mixing the obtained mixture and the green tea essence at a ratio of propyl hydroxybenzoate =1:2, and uniformly stirring at a high speed for 10 minutes to obtain the moisturizing and repairing facial cleanser containing the periplaneta americana extract.
The periplaneta americana extract is prepared by refining the liquid medicine and the water washing liquid discarded in the step E in the preparation process of the periplaneta americana extract disclosed in the Chinese patent 201210446628.5 according to the following process:
a. inactivation and crushing: killing fresh Periplaneta americana with 80 deg.C hot water, oven drying at 60 + -5 deg.C, pulverizing, and sieving with 24 mesh sieve to obtain Periplaneta americana coarse powder;
b. extraction: c, performing reflux extraction on the periplaneta americana coarse powder obtained in the step a for 3 times at the temperature of 75 ℃, and adding 90% ethanol in an amount which is 5 times the weight of the coarse powder during the first extraction for 4 hours; adding 90% ethanol with 3 times of the weight of the coarse powder for 3 hr each time; in the extraction process, the temperature of the ethanol is kept at 75 ℃, after the extraction is finished, the extracting solutions are combined and filtered to obtain an ethanol extract;
c. primary concentration: c, concentrating the ethanol extract obtained in the step b under reduced pressure at the temperature of 50-60 ℃ and the vacuum degree of-0.05-0.06 MPa until the specific gravity is 1.05 to obtain an ethanol concentrated solution;
d. degreasing and adsorbing: adding distilled water which is 7 times of the weight of the ethanol concentrated solution into the ethanol concentrated solution obtained in the step c, heating to 80 ℃ while stirring, stirring for 10 minutes at the stirring speed of 20 r/min, and then keeping the temperature and standing for 4 hours to separate oil from water; collecting lower layer water solution, filtering with filter paper, adding 0.5 times of medicinal active carbon into the filtrate, stirring at 40 deg.C for 50 min for full adsorption;
e. and (3) elution: d, filling the activated carbon-containing liquid medicine obtained in the step d into a chromatographic column, discharging the liquid medicine, washing with distilled water 10-30 times of the amount of the medicinal activated carbon, and collecting and uniformly mixing the liquid medicine and the washing liquid;
f. membrane separation: under the pressure of 200kPa, collecting the liquid medicine and the water washing liquid which are uniformly mixed in the step e, passing through an ultrafiltration ceramic membrane with the molecular cut-off value of 50kD, discarding the cut-off liquid, and collecting penetrating fluid;
g. secondary concentration: concentrating the penetrating fluid obtained in the step f under reduced pressure at the temperature of 50-60 ℃ and the vacuum degree of-0.05-0.06 MPa to 1/20 of the original volume;
h. ion exchange: taking the secondary concentrated solution obtained in the step g, using hydrochloric acid with the concentration of 1mol/L and sodium hydroxide with the concentration of 1mol/L to adjust the pH value of the concentrated penetrating fluid obtained in the step g to be 5, then passing through a regenerated sodium type HD-8 strong-acid styrene type cation exchange resin column, using sodium type HD-8 strong-acid styrene type cation exchange resin which is 4 times of the concentrated penetrating fluid obtained in the step g according to the volume, after the adsorption of the sodium type HD-8 strong-acid styrene type cation exchange resin column is finished, firstly using deionized water with the volume of 2 times of the amount of the sodium type HD-8 strong-acid styrene type cation exchange resin, then using ammonia water with the concentration of 1mol/L to elute, wherein the using amount of the ammonia water with the concentration of 1.5mol/L is 6 times of the amount of the sodium type HD-8 strong-acid styrene type cation exchange resin, controlling the flow rate of the eluent to ensure that the time of the whole elution process is 10 hours, and collecting the eluent;
i. and (3) third-stage concentration: and (5) concentrating the eluent obtained in the step h under reduced pressure at the temperature of 50-60 ℃ and the vacuum degree of-0.05-0.06 MPa to obtain an extract with the specific gravity of 1.24, thus obtaining the periplaneta americana refined extract.
The comprehensive effect of the moisturizing and repairing facial cleanser containing the periplaneta americana extract prepared in the embodiment is investigated and shown in table 1. The American cockroach refined extract contains up to 26 pure natural small molecular amino acids, and the total amino acid content in the refined extract is up to 62.3%.
Example 2
The facial cleanser containing the periplaneta americana refined extract for moisturizing and repairing consists of the following components in percentage by weight: 0.5% of periplaneta americana refined extract, 1% of prinsepia utilis royle oil, acetylated lanolin alcohol: triterpene alcohol =3:25 mixing the obtained mixture 1%, alkyl glycoside 2%, fatty alcohol-polyoxypropylene ether 2%, sodium fatty alcohol-polyoxyethylene ether sulfate 1%, glycerin 3%, sorbitol 1, 2-pentanediol =7:4, mixing the obtained mixture 3%, sodium N-acyl amino acid 10%, EDTA-Na4Amount 1%, DMDM hydantoin: propyl hydroxybenzoate =1:2, 0.2% of the mixture obtained, 0.4% of cucumber essence and the balance water.
The moisturizing and repairing facial cleanser containing the periplaneta americana refined extract is prepared by the following process:
s1, weighing the components for producing the facial cleanser according to the parts by weight;
s2, taking prinsepia utilis royle oil and acetylated lanolin alcohol: triterpene alcohol =3:2.5 mixing the obtained mixture, alkyl glycoside, fatty alcohol polyoxypropylene ether and fatty alcohol polyoxyethylene ether sodium sulfate, placing the mixture in an oil phase pot, stirring and heating to 60 ℃, and uniformly obtaining an oil phase A;
s3, putting water into a water phase pot, adding glycerol, sorbitol 1, 2-pentanediol =7:4, mixing the obtained mixture, sodium N-acyl amino acid and EDTA-Na4Stirring and heating to 70 ℃, and uniformly stirring to obtain a water phase B;
s4, uniformly mixing the oil phase A and the water phase B to obtain a material C;
s5, cooling the material C to 40 ℃, and adding the periplaneta americana refined extract and the DMDM hydantoin in proportion: and (3) mixing the obtained mixture and the cucumber essence at a ratio of propyl hydroxybenzoate =1:2, and uniformly stirring at a high speed for 20 minutes to obtain the moisturizing and repairing facial cleanser containing the periplaneta americana essence extract.
The periplaneta americana extract is prepared by refining the liquid medicine and the water washing liquid discarded in the step E in the preparation process of the periplaneta americana extract disclosed in the Chinese patent 201210446628.5 according to the following process:
a. inactivation and crushing: killing fresh Periplaneta americana with hot water of 90 deg.C, oven drying at 60 + -5 deg.C, pulverizing, and sieving with 24 mesh sieve to obtain Periplaneta americana coarse powder;
b. extraction: c, performing reflux extraction on the periplaneta americana coarse powder obtained in the step a for 5 times at the temperature of 80 ℃, and adding 95% ethanol with the concentration being 8 times of the weight of the coarse powder during the first extraction, wherein the extraction time is 6 hours; adding 95% ethanol 6 times the weight of the coarse powder for 5 hr each time; in the extraction process, the temperature of ethanol is kept at 80 ℃, after the extraction is finished, the extracting solutions are combined and filtered to obtain an ethanol extract;
c. primary concentration: c, concentrating the ethanol extract obtained in the step b under reduced pressure at the temperature of 50-60 ℃ and the vacuum degree of-0.05-0.06 MPa until the specific gravity is 1.15 to obtain an ethanol concentrated solution;
d. degreasing and adsorbing: adding distilled water which is 10 times of the weight of the ethanol concentrated solution into the ethanol concentrated solution obtained in the step c, heating to 90 ℃ while stirring, stirring for 60 minutes at the stirring speed of 60 revolutions per minute, and then keeping the temperature and standing for 5 hours to separate oil from water; collecting lower layer water solution, filtering with filter paper, adding 1.0 times of medicinal active carbon into the filtrate, stirring at 60 deg.C for 60 min for full adsorption;
e. and (3) elution: d, filling the liquid medicine containing the activated carbon obtained in the step d into a chromatographic column, discharging the liquid medicine, washing the liquid medicine with distilled water 30 times of the amount of the medicinal activated carbon, and collecting and uniformly mixing the liquid medicine and the washing liquid;
f. membrane separation: under the pressure of 50kPa, collecting the liquid medicine and the water washing liquid which are uniformly mixed in the step e, passing through an ultrafiltration ceramic membrane with the molecular cut-off value of 50kD, discarding the cut-off liquid, and collecting penetrating fluid;
g. secondary concentration: concentrating the penetrating fluid obtained in the step f under reduced pressure at the temperature of 50-60 ℃ and the vacuum degree of-0.05-0.06 MPa to 1/10 of the original volume;
h. ion exchange: taking the secondary concentrated solution obtained in the step g, using hydrochloric acid with the concentration of 1mol/L and sodium hydroxide with the concentration of 1mol/L to adjust the pH value of the concentrated penetrating fluid obtained in the step g to be 7, then passing through a regenerated sodium type HD-8 strong-acid styrene type cation exchange resin column, using sodium type HD-8 strong-acid styrene type cation exchange resin which is 7 times of the concentrated penetrating fluid obtained in the step g according to the volume, after the adsorption of the sodium type HD-8 strong-acid styrene type cation exchange resin column is finished, firstly using deionized water with the volume of 4 times of the amount of the sodium type HD-8 strong-acid styrene type cation exchange resin, then using ammonia water with the concentration of 3mol/L to elute, wherein the usage amount of the ammonia water with the concentration of 3mol/L is 8 times of the amount of the sodium type HD-8 strong-acid styrene type cation exchange resin, controlling the flow rate of the eluent to ensure that the time of the whole elution process is 12 hours, and collecting the eluent;
i. and (3) third-stage concentration: and (5) concentrating the eluent obtained in the step h under reduced pressure at the temperature of 50-60 ℃ and the vacuum degree of-0.05-0.06 MPa to obtain an extract with the specific gravity of 1.30, thus obtaining the periplaneta americana refined extract.
The comprehensive effect of the moisturizing and repairing facial cleanser containing the periplaneta americana extract prepared in the embodiment is investigated and shown in table 1. The American cockroach refined extract contains up to 26 pure natural small molecular amino acids, and the total amino acid content in the refined extract is up to 68.2%.
Example 3
The facial cleanser containing the periplaneta americana refined extract for moisturizing and repairing consists of the following components in percentage by weight: 3% of periplaneta americana refined extract, 5% of prinsepia utilis royle oil, and acetylated lanolin alcohol: triterpene alcohol =3:2.5 mixture 5%, alkyl glycoside 10%, fatty alcohol polyoxypropylene ether 7%, fatty alcohol polyoxyethylene ether sodium sulfate 6%, glycerin 10%, sorbitol 1, 2-pentanediol =7:4 mixture 10%, sodium N-acyl amino acid 20%, EDTA-Na4Amount 3%, DMDM hydantoin: propylparaben =1:2 the resulting mixture is mixed 1%, aloe vera essence 0.5%, and balance water.
The moisturizing and repairing facial cleanser containing the periplaneta americana refined extract is prepared by the following process:
s1, weighing the components for producing the facial cleanser according to the parts by weight;
s2, taking prinsepia utilis royle oil and acetylated lanolin alcohol: triterpene alcohol =3:2.5 mixing the obtained mixture, alkyl glycoside, fatty alcohol polyoxypropylene ether and fatty alcohol polyoxyethylene ether sodium sulfate, placing the mixture in an oil phase pot, stirring and heating to 50 ℃, and uniformly obtaining an oil phase A;
s3, putting water into a water phase pot, adding glycerol, sorbitol 1, 2-pentanediol =7:4, mixing the obtained mixture, sodium N-acyl amino acid and EDTA-Na4Stirring and heating to 65 ℃, and uniformly stirring to obtain a water phase B;
s4, uniformly mixing the oil phase A and the water phase B to obtain a material C;
s5, cooling the material C to 35 ℃, and adding the periplaneta americana refined extract and the DMDM hydantoin in proportion: and (3) mixing the obtained mixture and the aloe essence at a ratio of propyl hydroxybenzoate =1:2, and uniformly stirring at a high speed for 15 minutes to obtain the moisturizing and repairing facial cleanser containing the periplaneta americana extract.
The periplaneta americana extract is prepared by refining the liquid medicine and the water washing liquid discarded in the step E in the preparation process of the periplaneta americana extract disclosed in the Chinese patent 201210446628.5 according to the following process:
a. inactivation and crushing: killing fresh Periplaneta americana with 85 deg.C hot water, oven drying at 60 + -5 deg.C, pulverizing, and sieving with 24 mesh sieve to obtain Periplaneta americana coarse powder;
b. extraction: c, performing reflux extraction on the periplaneta americana coarse powder obtained in the step a for 4 times at the temperature of 75 ℃, and adding 90% ethanol in an amount which is 6 times the weight of the coarse powder when the coarse powder is extracted for 5 hours; adding 90% ethanol with a concentration 4 times the weight of the coarse powder for 4 hr each time; in the extraction process, the temperature of ethanol is kept at 80 ℃, after the extraction is finished, the extracting solutions are combined and filtered to obtain an ethanol extract;
c. primary concentration: c, concentrating the ethanol extract obtained in the step b under reduced pressure at the temperature of 50-60 ℃ and the vacuum degree of-0.05-0.06 MPa until the specific gravity is 1.1 to obtain an ethanol concentrated solution;
d. degreasing and adsorbing: c, adding distilled water which is 8 times of the weight of the ethanol concentrated solution into the ethanol concentrated solution obtained in the step c, heating to 85 ℃ while stirring, stirring for 40 minutes at the stirring speed of 40 revolutions per minute, and then keeping the temperature and standing for 3 hours to separate oil from water; collecting lower layer water solution, filtering with filter paper, adding 0.6 times of medicinal active carbon into the filtrate, stirring at 50 deg.C for 50 min for full adsorption;
e. and (3) elution: d, filling the liquid medicine containing the activated carbon obtained in the step d into a chromatographic column, discharging the liquid medicine, washing the liquid medicine with distilled water 20 times of the amount of the medicinal activated carbon, and collecting and uniformly mixing the liquid medicine and the washing liquid;
f. membrane separation: under the pressure of 150kPa, collecting the liquid medicine and the water washing liquid which are uniformly mixed in the step e, passing through an ultrafiltration ceramic membrane with the molecular cut-off value of 50kD, discarding the cut-off liquid, and collecting penetrating fluid;
g. secondary concentration: concentrating the penetrating fluid obtained in the step f under reduced pressure at the temperature of 50-60 ℃ and the vacuum degree of-0.05-0.06 MPa to 1/30 of the original volume;
h. ion exchange: taking the secondary concentrated solution obtained in the step g, using hydrochloric acid with the concentration of 1mol/L and sodium hydroxide with the concentration of 1mol/L to adjust the pH value of the concentrated penetrating fluid obtained in the step g to be 6, then passing through a regenerated sodium type HD-8 strong-acid styrene type cation exchange resin column, using sodium type HD-8 strong-acid styrene type cation exchange resin which is 5 times of the concentrated penetrating fluid obtained in the step g according to the volume, after the adsorption of the sodium type HD-8 strong-acid styrene type cation exchange resin column is finished, firstly using deionized water with the volume of 3 times of the amount of the sodium type HD-8 strong-acid styrene type cation exchange resin, then using ammonia water with the concentration of 1.5mol/L to elute, wherein the using amount of the ammonia water with the concentration of 1.5mol/L is 6 times of the amount of the sodium type HD-8 strong-acid styrene type cation exchange resin, controlling the flow rate of the eluent to ensure that the time of the whole elution process is 10 hours, and collecting the eluent;
i. and (3) third-stage concentration: and (5) concentrating the eluent obtained in the step h under reduced pressure at the temperature of 50-60 ℃ and the vacuum degree of-0.05-0.06 MPa to obtain an extract with the specific gravity of 1.25, thus obtaining the periplaneta americana refined extract.
The comprehensive effect of the moisturizing and repairing facial cleanser containing the periplaneta americana extract prepared in the embodiment is investigated and shown in table 1. The American cockroach refined extract contains up to 26 pure natural small molecular amino acids, and the total amino acid content in the refined extract is up to 73.9%.
Example 4
The facial cleanser containing the periplaneta americana refined extract for moisturizing and repairing consists of the following components in percentage by weight: 0.9% of periplaneta americana refined extract, 1.8% of prinsepia utilis royle oil, acetylated lanolin alcohol: triterpene alcohol =3:2.5 mixture 2.5%, alkyl glycoside 6%, fatty alcohol polyoxypropylene ether 2%, sodium fatty alcohol polyoxyethylene ether sulfate 3.5%, glycerin 6%, sorbitol 1, 2-pentanediol =7:4 mixture 7%, sodium N-acyl amino acid 15%, EDTA-Na4Amount 1.5%, DMDM hydantoin: propyl hydroxybenzoate =1:2 blendAnd 0.4% of the mixture, 0.1% of green tea essence and the balance of water.
The moisturizing and repairing facial cleanser containing the periplaneta americana refined extract is prepared by the following process:
s1, weighing the components for producing the facial cleanser according to the parts by weight;
s2, taking prinsepia utilis royle oil and acetylated lanolin alcohol: triterpene alcohol =3:2.5 mixing the obtained mixture, alkyl glycoside, fatty alcohol polyoxypropylene ether and fatty alcohol polyoxyethylene ether sodium sulfate, placing the mixture in an oil phase pot, stirring and heating to 50 ℃, and uniformly obtaining an oil phase A;
s3, putting water into a water phase pot, adding glycerol, sorbitol 1, 2-pentanediol =7:4, mixing the obtained mixture, sodium N-acyl amino acid and EDTA-Na4Stirring and heating to 70 ℃, and uniformly stirring to obtain a water phase B;
s4, uniformly mixing the oil phase A and the water phase B to obtain a material C;
s5, cooling the material C to 30 ℃, and adding the periplaneta americana refined extract and the DMDM hydantoin in proportion: and (3) mixing the obtained mixture and the green tea essence at a ratio of propyl hydroxybenzoate =1:2, and uniformly stirring at a high speed for 10 minutes to obtain the moisturizing and repairing facial cleanser containing the periplaneta americana extract.
The periplaneta americana extract is prepared by refining the liquid medicine and the water washing liquid discarded in the step E in the preparation process of the periplaneta americana extract disclosed in the Chinese patent 201210446628.5 according to the following process:
a. inactivation and crushing: killing fresh Periplaneta americana with hot water at 80 deg.C, oven drying at 60 + -5 deg.C, pulverizing, and sieving with 24 mesh sieve to obtain Periplaneta americana coarse powder;
b. extraction: c, performing reflux extraction on the periplaneta americana coarse powder obtained in the step a for 5 times at the temperature of 75 ℃, and adding 95% ethanol with the concentration being 8 times of the weight of the coarse powder during the first extraction, wherein the extraction time is 4 hours; adding 95% ethanol 4 times the weight of the coarse powder for 4 hr each time; in the extraction process, the temperature of the ethanol is kept at 75 ℃, after the extraction is finished, the extracting solutions are combined and filtered to obtain an ethanol extract;
c. primary concentration: c, concentrating the ethanol extract obtained in the step b under reduced pressure at the temperature of 50-60 ℃ and the vacuum degree of-0.05-0.06 MPa until the specific gravity is 1.15 to obtain an ethanol concentrated solution;
d. degreasing and adsorbing: adding distilled water which is 10 times of the weight of the ethanol concentrated solution into the ethanol concentrated solution obtained in the step c, heating to 85 ℃ while stirring, stirring for 30 minutes at the stirring speed of 30 revolutions per minute, and then keeping the temperature and standing for 5 hours to separate oil from water; collecting lower layer water solution, filtering with filter paper, adding 1.0 times of medicinal active carbon into the filtrate, stirring at 40 deg.C for 60 min for full adsorption;
e. and (3) elution: d, filling the liquid medicine containing the activated carbon obtained in the step d into a chromatographic column, discharging the liquid medicine, washing the liquid medicine with distilled water 20 times of the amount of the medicinal activated carbon, and collecting and uniformly mixing the liquid medicine and the washing liquid;
f. membrane separation: under the pressure of 80kPa, collecting the liquid medicine and the water washing liquid which are uniformly mixed in the step e, passing through an ultrafiltration ceramic membrane with the molecular cut-off value of 50kD, discarding the cut-off liquid, and collecting penetrating fluid;
g. secondary concentration: concentrating the penetrating fluid obtained in the step f under reduced pressure at the temperature of 50-60 ℃ and the vacuum degree of-0.05-0.06 MPa to 1/20 of the original volume;
h. ion exchange: taking the secondary concentrated solution obtained in the step g, regulating the pH value of the concentrated penetrating fluid obtained in the step g to be 6.0 by using hydrochloric acid with the concentration of 1mol/L and sodium hydroxide with the concentration of 1mol/L, then passing through a regenerated sodium type HD-8 strong-acid styrene type cation exchange resin column, washing the sodium type HD-8 strong-acid styrene type cation exchange resin with deionized water with the volume of 3 times of the amount of the sodium type HD-8 strong-acid styrene type cation exchange resin after the adsorption of the sodium type HD-8 strong-acid styrene type cation exchange resin column is finished, then eluting by using ammonia water with the concentration of 1-3mol/L, wherein the using amount of the ammonia water with the concentration of 1.5mol/L is 6 times of the amount of the sodium type HD-8 strong-acid styrene type cation exchange resin, controlling the flow rate of the eluent to ensure that the time of the whole elution process is 10 hours, and collecting the eluent.
i. And (3) third-stage concentration: and (5) concentrating the eluent obtained in the step h under reduced pressure at the temperature of 50-60 ℃ and the vacuum degree of-0.05-0.06 MPa to obtain an extract with the specific gravity of 1.21, thus obtaining the periplaneta americana refined extract.
The comprehensive effect of the moisturizing and repairing facial cleanser containing the periplaneta americana extract prepared in the embodiment is investigated and shown in table 1. The American cockroach refined extract contains up to 26 pure natural small molecular amino acids, and the total amino acid content in the refined extract is up to 79.7%.
Example 5
The facial cleanser containing the periplaneta americana refined extract for moisturizing and repairing consists of the following components in percentage by weight: 2% of periplaneta americana refined extract, 3% of prinsepia utilis royle oil, and acetylated lanolin alcohol: triterpene alcohol =3:2.5 mixture 4%, alkyl glycoside 9%, fatty alcohol polyoxypropylene ether 4%, sodium fatty alcohol polyoxyethylene ether sulfate 4.5%, glycerin 9%, sorbitol 1, 2-pentanediol =7:4 mixture 7%, sodium N-acyl amino acid 18%, EDTA-Na4Amount 2.5%, DMDM hydantoin: propyl hydroxybenzoate =1:2, 0.8% of the mixture obtained, 0.3% of cucumber essence and the balance water.
The moisturizing and repairing facial cleanser containing the periplaneta americana refined extract is prepared by the following process:
s1, weighing the components for producing the facial cleanser according to the parts by weight;
s2, taking prinsepia utilis royle oil and acetylated lanolin alcohol: triterpene alcohol =3:2.5 mixing the obtained mixture, alkyl glycoside, fatty alcohol polyoxypropylene ether and fatty alcohol polyoxyethylene ether sodium sulfate, placing the mixture in an oil phase pot, stirring and heating to 50 ℃, and uniformly obtaining an oil phase A;
s3, putting water into a water phase pot, adding glycerol, sorbitol 1, 2-pentanediol =7:4, mixing the obtained mixture, sodium N-acyl amino acid and EDTA-Na4Stirring and heating to 70 ℃, and uniformly stirring to obtain a water phase B;
s4, uniformly mixing the oil phase A and the water phase B to obtain a material C;
s5, cooling the material C to 30 ℃, and adding the periplaneta americana refined extract and the DMDM hydantoin in proportion: and (3) mixing the obtained mixture and the cucumber essence at a ratio of propyl hydroxybenzoate =1:2, and uniformly stirring at a high speed for 10 minutes to obtain the moisturizing and repairing facial cleanser containing the periplaneta americana essence extract.
The periplaneta americana extract is prepared by refining the liquid medicine and the water washing liquid discarded in the step E in the preparation process of the periplaneta americana extract disclosed in the Chinese patent 201210446628.5 according to the following process:
a. inactivation and crushing: killing fresh Periplaneta americana with hot water at 80 deg.C, oven drying at 60 + -5 deg.C, pulverizing, and sieving with 24 mesh sieve to obtain Periplaneta americana coarse powder;
b. extraction: c, performing reflux extraction on the periplaneta americana coarse powder obtained in the step a for 5 times at the temperature of 75 ℃, and adding 95% ethanol with the concentration being 8 times of the weight of the coarse powder during the first extraction, wherein the extraction time is 4 hours; adding 95% ethanol 4 times the weight of the coarse powder for 4 hr each time; in the extraction process, the temperature of the ethanol is kept at 75 ℃, after the extraction is finished, the extracting solutions are combined and filtered to obtain an ethanol extract;
c. primary concentration: c, concentrating the ethanol extract obtained in the step b under reduced pressure at the temperature of 50-60 ℃ and the vacuum degree of-0.05-0.06 MPa until the specific gravity is 1.15 to obtain an ethanol concentrated solution;
d. degreasing and adsorbing: adding distilled water which is 10 times of the weight of the ethanol concentrated solution into the ethanol concentrated solution obtained in the step c, heating to 85 ℃ while stirring, stirring for 30 minutes at the stirring speed of 30 revolutions per minute, and then keeping the temperature and standing for 5 hours to separate oil from water; collecting lower layer water solution, filtering with filter paper, adding 1.0 times of medicinal active carbon into the filtrate, stirring at 40 deg.C for 60 min for full adsorption;
e. and (3) elution: d, filling the liquid medicine containing the activated carbon obtained in the step d into a chromatographic column, discharging the liquid medicine, washing the liquid medicine with distilled water 20 times of the amount of the medicinal activated carbon, and collecting and uniformly mixing the liquid medicine and the washing liquid;
f. membrane separation: under the pressure of 120kPa, collecting the liquid medicine and the water washing liquid which are uniformly mixed in the step e, passing through an ultrafiltration ceramic membrane with the molecular cut-off value of 50kD, discarding the cut-off liquid, and collecting penetrating fluid;
g. secondary concentration: concentrating the penetrating fluid obtained in the step f under reduced pressure at the temperature of 50-60 ℃ and the vacuum degree of-0.05-0.06 MPa to 1/20 of the original volume;
h. ion exchange: taking the secondary concentrated solution obtained in the step g, regulating the pH value of the concentrated penetrating fluid obtained in the step g to be 6.0 by using hydrochloric acid with the concentration of 1mol/L and sodium hydroxide with the concentration of 1mol/L, then passing through a regenerated sodium type HD-8 strong-acid styrene type cation exchange resin column, washing the sodium type HD-8 strong-acid styrene type cation exchange resin with deionized water with the volume of 3 times of the amount of the sodium type HD-8 strong-acid styrene type cation exchange resin after the adsorption of the sodium type HD-8 strong-acid styrene type cation exchange resin column is finished, then eluting by using ammonia water with the concentration of 1-3mol/L, wherein the using amount of the ammonia water with the concentration of 1.5mol/L is 6 times of the amount of the sodium type HD-8 strong-acid styrene type cation exchange resin, controlling the flow rate of the eluent to ensure that the time of the whole elution process is 10 hours, and collecting the eluent.
i. And (3) third-stage concentration: and (5) concentrating the eluent obtained in the step h under reduced pressure at the temperature of 50-60 ℃ and the vacuum degree of-0.05-0.06 MPa to obtain an extract with the specific gravity of 1.28, thus obtaining the periplaneta americana refined extract.
The comprehensive effect of the moisturizing and repairing facial cleanser containing the periplaneta americana extract prepared in the embodiment is investigated and shown in table 1. The American cockroach refined extract contains up to 26 pure natural small molecular amino acids, and the total amino acid content in the refined extract is up to 79.7%.
Example 6
The facial cleanser containing the periplaneta americana refined extract for moisturizing and repairing consists of the following components in percentage by weight: 1% of periplaneta americana refined extract, 2% of prinsepia utilis royle oil, and acetylated lanolin alcohol: triterpene alcohol =3:2.5 mixture 3%, alkyl glycoside 8%, fatty alcohol polyoxypropylene ether 2.5%, sodium fatty alcohol polyoxyethylene ether sulfate 4%, glycerin 7%, sorbitol 1, 2-pentanediol =7:4 mixture 7%, sodium N-acyl amino acid 17%, EDTA-Na4Amount 2%, DMDM hydantoin: propyl hydroxybenzoate =1:2 the mixture was 0.6%, aloe vera essence 0.25%, and balance water.
The moisturizing and repairing facial cleanser containing the periplaneta americana refined extract is prepared by the following process:
s1, weighing the components for producing the facial cleanser according to the parts by weight;
s2, taking prinsepia utilis royle oil and acetylated lanolin alcohol: triterpene alcohol =3:2.5 mixing the obtained mixture, alkyl glycoside, fatty alcohol polyoxypropylene ether and fatty alcohol polyoxyethylene ether sodium sulfate, placing the mixture in an oil phase pot, stirring and heating to 50 ℃, and uniformly obtaining an oil phase A;
s3, putting water into a water phase pot, adding glycerol, sorbitol 1, 2-pentanediol =7:4, mixing the obtained mixture, sodium N-acyl amino acid and EDTA-Na4Stirring and heating to 70 ℃, and uniformly stirring to obtain a water phase B;
s4, uniformly mixing the oil phase A and the water phase B to obtain a material C;
s5, cooling the material C to 30 ℃, and adding the periplaneta americana refined extract and the DMDM hydantoin in proportion: and (3) mixing the obtained mixture and the aloe essence at a ratio of 1:2, and uniformly stirring at a high speed for 10 minutes to obtain the moisturizing and repairing facial cleanser containing the periplaneta americana extract.
The periplaneta americana extract is prepared by refining the liquid medicine and the water washing liquid discarded in the step E in the preparation process of the periplaneta americana extract disclosed in the Chinese patent 201210446628.5 according to the following process:
a. inactivation and crushing: killing fresh Periplaneta americana with 85 deg.C hot water, oven drying at 60 + -5 deg.C, pulverizing, and sieving with 24 mesh sieve to obtain Periplaneta americana coarse powder;
b. extraction: c, performing reflux extraction on the periplaneta americana coarse powder obtained in the step a for 3 times at the temperature of 80 ℃, and adding 95% ethanol in an amount which is 7 times the weight of the coarse powder during the first extraction for 6 hours; adding 95% ethanol 6 times the weight of the coarse powder for 4 hr each time; in the extraction process, the temperature of the ethanol is kept at 75 ℃, after the extraction is finished, the extracting solutions are combined and filtered to obtain an ethanol extract;
c. primary concentration: c, concentrating the ethanol extract obtained in the step b under reduced pressure under the conditions of vacuum degree of-0.06 MPa and temperature of 55 ℃ until the specific gravity is 1.05 to obtain an ethanol concentrated solution;
d. degreasing and adsorbing: adding distilled water which is 10 times of the weight of the ethanol concentrated solution into the ethanol concentrated solution obtained in the step c, heating to 85 ℃ while stirring, stirring for 40 minutes at the stirring speed of 40 revolutions per minute, and then keeping the temperature and standing for 5 hours to separate oil from water; collecting lower layer water solution, filtering with filter paper, adding ethanol concentrated solution into the filtrate, and stirring with 0 times of medicinal active carbon at 45 deg.C for 40 min for full adsorption;
e. and (3) elution: d, filling the liquid medicine containing the activated carbon obtained in the step d into a chromatographic column, discharging the liquid medicine, washing the liquid medicine with distilled water 15 times of the medicinal activated carbon, and collecting and uniformly mixing the liquid medicine and the washing liquid;
f. membrane separation: passing through ultrafiltration ceramic membrane with molecular cut-off of 50kD under 80kPa, discarding the cut-off solution, and collecting the penetrating fluid.
g. Secondary concentration: and f, concentrating the penetrating fluid obtained in the step f under reduced pressure at the temperature of 50-60 ℃ and the vacuum degree of-0.05-0.06 MPa to 1/20 of the original volume.
h. Ion exchange: taking the secondary concentrated solution obtained in the step g, regulating the pH value of the concentrated penetrating fluid obtained in the step g to be 6.0 by using hydrochloric acid with the concentration of 1mol/L and sodium hydroxide with the concentration of 1mol/L, then passing through a regenerated sodium type HD-8 strong-acid styrene type cation exchange resin column, washing the sodium type HD-8 strong-acid styrene type cation exchange resin with deionized water with the volume of 3 times of the amount of the sodium type HD-8 strong-acid styrene type cation exchange resin after the adsorption of the sodium type HD-8 strong-acid styrene type cation exchange resin column is finished, then eluting by using ammonia water with the concentration of 1-3mol/L, wherein the using amount of the ammonia water with the concentration of 1.5mol/L is 6 times of the amount of the sodium type HD-8 strong-acid styrene type cation exchange resin, controlling the flow rate of the eluent to ensure that the time of the whole elution process is 10 hours, and collecting the eluent.
i. And (3) third-stage concentration: and (5) concentrating the eluent obtained in the step h under reduced pressure at the temperature of 50-60 ℃ and the vacuum degree of-0.05-0.06 MPa to obtain an extract with the specific gravity of 1.30, thus obtaining the periplaneta americana refined extract.
The comprehensive effect of the moisturizing and repairing facial cleanser containing the periplaneta americana extract prepared in the embodiment is investigated and shown in table 1. The American cockroach refined extract contains up to 26 pure natural small molecular amino acids, and the total amino acid content in the refined extract is up to 79.8%.
Examination of comprehensive effects of facial cleanser
The efficacy of the facial cleansers manufactured in examples 1 to 6 was evaluated by the trial feeling. The subjects were 120 persons in total, 35 men and 85 women, and the ages were 25 to 60 years old. The using method comprises the following steps: the tested subject directly uses the tested product once a day in the morning and at night, the face is wetted with clean water before use, a proper amount of the facial cleanser is squeezed out of the palm, a small amount of clean water is added to rub the face to obtain rich foam, and the face is washed clean with clean water after being massaged for a moment. The patient keeps on using the traditional Chinese medicine for one month, and is checked at least once a week to know whether systemic and local adverse reactions exist or not, and the result is recorded in detail. The using effects of the ingredients are divided into 5 points: a score of 5 is the highest score, indicating very good, very satisfactory; 4, the classification is very good; 3 is acceptable; when the amount is less than 3 points, the results are not acceptable. The average scores are given below and the results are given in table 1 below:
Figure DEST_PATH_IMAGE002
the facial cleanser has the advantages that the whole feeling is fresh and non-greasy, the facial cleanser can effectively clean facial dirt, the skin can be kept moist and non-dry for a long time, most subjects can still respond to the facial cleanser after being washed, and the repairing effect on the damaged skin of the face is better after the facial cleanser is washed before sleep. After long-time use, the facial skin is obviously improved, the injured scar is obviously healed, and the testee has no local skin adverse reaction and has no general adverse reaction such as anorexia, diarrhea, hypodynamia, endocrine disturbance and the like.
In conclusion, the moisturizing and repairing facial cleanser containing the periplaneta americana essence extract has good skin permeability, can deeply clean the skin, can promote the repair of damaged cells, promote the growth of new cells, improve the degeneration of cutin, diminish inflammation, inhibit bacteria, resist ultraviolet rays and aging, improve the circulation of crinkles of human bodies, has the effects of locking water, moisturizing, preventing cracking and supplementing nutrition on the facial skin, and can better improve the elasticity of the facial skin.
The above is only a preferred embodiment of the present invention, and it should be noted that the above preferred embodiment should not be considered as limiting the present invention, and the protection scope of the present invention should be subject to the scope defined by the claims. It will be apparent to those skilled in the art that various modifications and adaptations can be made without departing from the spirit and scope of the invention, and these modifications and adaptations should be considered within the scope of the invention.

Claims (7)

1. The moisturizing and repairing facial cleanser containing the periplaneta americana refined extract is characterized by comprising the following components in percentage by weight:
0.5-3% of periplaneta americana refined extract, 1-5% of prinsepia utilis royle oil, and acetylated lanolin alcohol: triterpene alcohol =3:2.5, 1-5% of the mixture obtained by mixing, 2-10% of alkyl glycoside, 2-7% of fatty alcohol polyoxyethylene ether, 1-6% of fatty alcohol polyoxyethylene ether sodium sulfate, 3-10% of glycerol, and sorbitol: 3-10% of 1, 2-pentanediol =7:4, 10-20% of sodium N-acylamidoate, and EDTA-Na41-3% of dosage, DMDM hydantoin: propyl hydroxybenzoate =1:2, 0.2-1% of the mixture obtained by mixing, 0.1-0.5% of essence and the balance of water;
the periplaneta americana extract is prepared by refining the liquid medicine and the water washing liquid discarded in the step E in the preparation process of the periplaneta americana extract disclosed in the Chinese patent 201210446628.5 according to the following process:
a. inactivation and crushing: killing the fresh American cockroach by hot water at 80-90 ℃, controlling the temperature within 60 +/-5 ℃ for drying, crushing, and sieving by a 24-mesh sieve to prepare coarse American cockroach powder;
b. extraction: c, performing 3-5 times of reflux extraction on the periplaneta americana coarse powder obtained in the step a at the temperature of 75-80 ℃, and adding 90-95% ethanol in an amount which is 5-8 times the weight of the coarse powder during the first extraction for 4-6 hours; adding 90-95% ethanol in an amount which is 3-6 times the weight of the coarse powder every time after the second time, wherein the extraction time is 3-5 hours; in the extraction process, the temperature of ethanol is kept at 75-80 ℃, after extraction is finished, the extracting solutions are combined and filtered to obtain an ethanol extracting solution;
c. primary concentration: c, concentrating the ethanol extract obtained in the step b under reduced pressure under the conditions of vacuum degree of-0.05 to-0.06 MPa and temperature of 50 to 60 ℃ until the specific gravity is 1.05 to 1.15 to obtain an ethanol concentrated solution;
d. degreasing and adsorbing: adding distilled water which is 7-10 times of the weight of the ethanol concentrated solution into the ethanol concentrated solution obtained in the step c, heating to 80-90 ℃ while stirring, stirring for 10-60 minutes at a stirring speed of 20-60 revolutions per minute, and then keeping the temperature and standing for 1-5 hours to separate oil from water; collecting the lower-layer water solution, filtering the lower-layer water solution by using filter paper, adding medicinal active carbon which is 0.5 to 1.0 time of the ethanol concentrated solution into the obtained filtrate, and stirring the mixture at the temperature of between 40 and 60 ℃ for 30 to 60 minutes to ensure that the mixture is fully adsorbed;
e. and (3) elution: d, filling the activated carbon-containing liquid medicine obtained in the step d into a chromatographic column, discharging the liquid medicine, washing with distilled water 10-30 times of the amount of the medicinal activated carbon, and collecting and uniformly mixing the liquid medicine and the washing liquid;
f. membrane separation: under the pressure of 50-200kPa, collecting the liquid medicine and the water washing liquid which are uniformly mixed in the step e, passing through an ultrafiltration ceramic membrane with the molecular cut-off value of 50kD, discarding the cut-off liquid, and collecting penetrating fluid;
g. secondary concentration: c, concentrating the penetrating fluid obtained in the step a under reduced pressure at the temperature of 50-60 ℃ and the vacuum degree of-0.05-0.06 MPa to 1/10-1/30 of the original volume;
h. ion exchange: taking the secondary concentrated solution obtained in the step g, using hydrochloric acid with the concentration of 1mol/L and sodium hydroxide with the concentration of 1mol/L to adjust the pH value of the concentrated penetrating fluid obtained in the step g to be 5-7, then passing through a regenerated sodium type HD-8 strong-acid styrene type cation exchange resin column, using sodium type HD-8 strong-acid styrene type cation exchange resin which is 3-7 times of the concentrated penetrating fluid obtained in the step g according to the volume, after the adsorption of the sodium type HD-8 strong-acid styrene type cation exchange resin column is finished, firstly using deionized water with the volume of 2-4 times of the amount of the sodium type HD-8 strong-acid styrene type cation exchange resin, then using ammonia water solution with the concentration of 1-3mol/L to elute, wherein the using amount of the ammonia water solution with the concentration of 1-3mol/L is 5-8 times of the amount of the sodium type HD-8 strong-acid styrene type cation exchange resin, controlling the flow rate of the eluent to ensure that the time of the whole elution process is 8-12 hours, and collecting the eluent;
i. and (3) third-stage concentration: and (5) concentrating the eluent obtained in the step h under reduced pressure at the temperature of 50-60 ℃ and the vacuum degree of-0.05-0.06 MPa to obtain an extract with the specific gravity of 1.20-1.30, thus obtaining the periplaneta americana refined extract.
2. The moisturizing and repairing facial cleanser containing periplaneta americana refined extract according to claim 1 is characterized by comprising the following components in percentage by weight:
0.9-2% of periplaneta americana refined extract, 1.8-3% of prinsepia utilis royle oil, and acetylated lanolin alcohol: triterpene alcohol =3:2.5, 2.5-4% of the mixture obtained by mixing, 6-9% of alkyl glycoside, 2-4% of fatty alcohol polyoxypropylene ether, 3.5-4.5% of fatty alcohol polyoxyethylene ether sodium sulfate, 6-9% of glycerol, sorbitol: 1, 2-pentanediol =7:4, 7% of the resulting mixture, 15-18% of sodium N-acyl amino acid, and EDTA-Na41.5-2.5% of dosage, DMDM hydantoin: propyl hydroxybenzoate =1:2, 0.4-0.8% of the mixture obtained by mixing, 0.2-0.3% of essence and the balance of water.
3. The moisturizing and repairing facial cleanser containing periplaneta americana extract according to claim 1 or 2, wherein the essence is one or a mixture of green tea essence, cucumber essence and aloe essence.
4. The moisturizing and repairing facial cleanser containing periplaneta americana extract according to any one of claims 1 or 2, which is characterized in that the facial cleanser is prepared by the following process:
s1, weighing the components for producing the facial cleanser according to the parts by weight;
s2, taking prinsepia utilis royle oil and acetylated lanolin alcohol: triterpene alcohol =3:2.5 mixing the obtained mixture, alkyl glycoside, fatty alcohol polyoxypropylene ether and fatty alcohol polyoxyethylene ether sodium sulfate, placing the mixture in an oil phase pot, stirring and heating to 40-60 ℃, and uniformly obtaining an oil phase A;
s3, putting water into a water phase pot, adding glycerol and sorbitol: 1, 2-pentanediol =7:4 the resulting mixture, sodium N-acylamidoate, EDTA-Na were mixed4Stirring and heating to 60-70 ℃, and uniformly stirring to obtain a water phase B;
s4, uniformly mixing the oil phase A and the water phase B to obtain a material C;
s5, cooling the material C to 30-40 ℃, and adding the periplaneta americana refined extract and DMDM hydantoin in proportion: and (3) mixing the obtained mixture and the essence at a ratio of propyl hydroxybenzoate =1:2, and uniformly stirring at a high speed for 5-20 minutes to obtain the moisturizing and repairing facial cleanser containing the periplaneta americana extract.
5. The moisturizing and repairing facial cleanser containing periplaneta americana refined extract according to claim 1, wherein the process of step f is that under the pressure of 80kPa, the liquid medicine and the water washing liquid which are uniformly mixed are collected in step e and pass through an ultrafiltration ceramic membrane with the molecular cut-off value of 50kD, the cut-off liquid is discarded, and the penetrating fluid is collected.
6. The moisturizing and repairing facial cleanser containing periplaneta americana refined extract according to claim 1, wherein the process of step g is two-stage concentration: and f, concentrating the penetrating fluid obtained in the step f under reduced pressure at the temperature of 50-60 ℃ and the vacuum degree of-0.05-0.06 MPa to 1/20 of the original volume.
7. The moisturizing and repairing facial cleanser containing periplaneta americana refined extract according to claim 1, wherein the process of step h is as follows: taking the secondary concentrated solution obtained in the step g, regulating the pH value of the concentrated penetrating fluid obtained in the step g to be 6.0 by using hydrochloric acid with the concentration of 1mol/L and sodium hydroxide with the concentration of 1mol/L, then passing through a regenerated sodium type HD-8 strong-acid styrene type cation exchange resin column, washing the sodium type HD-8 strong-acid styrene type cation exchange resin with deionized water with the volume of 3 times of the amount of the sodium type HD-8 strong-acid styrene type cation exchange resin after the adsorption of the sodium type HD-8 strong-acid styrene type cation exchange resin column is finished, then eluting by using ammonia water with the concentration of 1-3mol/L, wherein the using amount of the ammonia water with the concentration of 1-3mol/L is 6 times of the amount of the sodium type HD-8 strong-acid styrene type cation exchange resin, controlling the flow rate of the eluent to ensure that the time of the whole elution process is 10 hours, and collecting the eluent.
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