CN1137629C - Continuous technolgical process of extracting soybean and separating protein, isoflavone, oligosaccharide and saponin - Google Patents
Continuous technolgical process of extracting soybean and separating protein, isoflavone, oligosaccharide and saponin Download PDFInfo
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- CN1137629C CN1137629C CNB00107606XA CN00107606A CN1137629C CN 1137629 C CN1137629 C CN 1137629C CN B00107606X A CNB00107606X A CN B00107606XA CN 00107606 A CN00107606 A CN 00107606A CN 1137629 C CN1137629 C CN 1137629C
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- Medicines Containing Plant Substances (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Beans For Foods Or Fodder (AREA)
Abstract
The present invention discloses a technology for continuously extracting soybean isolated protein, soybean isoflavone, soybean oligosaccharide and soybean saponin, which comprises: step (1), the extraction of soybean isolated protein, water is added to the raw materials, and the pH value is adjusted to 7 to 8; raw material liquid is stirred and precipitated to obtain supernatant liquid A and precipitation liquid B; the supernatant liquid A is passed a filtering machine to obtain protein solution C; the precipitation liquid B is lixiviated for twice to obtain protein solution D, protein solution G and protein solution I; the four parts of protein solution are mixed, separated with a separating machine and dried by spray to obtain soybean isolated protein; step (2), the extraction of soybean isoflavone; step (3), the extraction of soybean oligosaccharide; step (4), the extraction of soybean saponin. Because the technology of the present invention can be used for continuously extracting four kinds of products, the technology can reduce waste and cost; the technology has a good application prospect.
Description
The invention belongs to soybean deep processing field.
Soybean protein isolate, soybean isoflavones, soybean oligosaccharide and Soyasaponin are several important component that contain in the soybean seeds.Soybean protein isolate is rich in eight kinds of indispensable amino acids and other non-essential amino acid such as Methionin, tryptophane, Xie Ansuan, have characteristics such as water-retentivity, tackiness, gelation, oil absorbency and emulsifying property, can extensively be used as the basic material of numerous food, also can directly enter the consumption market, for the human consumer provides good vegetable-protein, can also be used widely in fields such as feed processing, pharmacy, plastics industries.Soybean isoflavones can be converted into the compound that is similar to hormone by bacterium in the intestines, can block blood vessel hyperplasia effectively in the breeding of malignant tumour, and breaks off the nutriment source, thereby delays or stop neoplastic disease to become cancer.Simultaneously, soybean isoflavones can also directly suppress the unusual again of bone, preventing osteoporosis disease, and have antimycotic, prevent and treat effects such as hot flush, so soybean isoflavones will play important effect in pharmacy and medical health field, because extract the method complexity of isoflavones, cost is too high, thus at present on the world market price of every gram soybean isoflavones up to 1390 dollars.Soybean oligosaccharide can not be absorbed in people's digestive tube, and can be used as does not have the sweeting agent of energy to use, and can promote the propagation of intestinal beneficial bacterium, improve environment in the intestines, have defaecation, the anti-effect that treats constipation is used widely in food, pharmacy and health care industry.Soyasaponin has removes free radical, anti-oxidant and reduce the effect of lipid peroxide, can suppress the oxidation of lipid in the serum, reduces the content of blood cholesterol and triglyceride.Soyasaponin can also stop growth of tumour cell, suppresses the infection of hiv virus, and infected cells is had the certain protection effect, has broad application prospects in pharmacy and medical health field.
At present, when soybean deep processing enterprise produces soybean protein isolate, soybean isoflavones, soybean oligosaccharide and Soyasaponin, because the method complexity is with high content of technology, generally all uses single method and technology, produce single kind, in the waste behind the extraction object, often still contain the material of a lot of preciousnesses, caused the very big wasting of resources, simultaneously make product cost too high, the market competitiveness is not strong yet.
The method that the purpose of this invention is to provide a kind of continuous production soybean protein isolate, soybean isoflavones, soybean oligosaccharide and Soyasaponin.
The present invention takes following design: the method for a kind of continuous extraction soybean protein isolate, soybean isoflavones, soybean oligosaccharide and Soyasaponin, consist essentially of following steps,
(1), extracts soybean protein isolate
1., raw material: the soybean plumular axis of defatted soybean meal and pulverizing;
2., put in the multi-functional lixiviate stirred pot after adding entry in the raw material, the weight ratio of raw material and water is 1: 10-15;
3., the lixiviate raw material in the multi-functional lixiviate stirred pot is transferred to pH7-8, stir under the 40-50 ℃ of condition, natural subsidence obtains supernatant A and precipitated liquid B, and supernatant A is separated with precipitated liquid B;
4., supernatant A is filtered by filter, obtain protein solution C;
5., add entry among the precipitated liquid B, the weight ratio of precipitated liquid B and water is 1: 7-10, this liquid is transferred pH to 7-8, and stir under the 40-50 ℃ of condition, natural subsidence obtains albumin soln D and precipitated liquid E, will go up albumin soln D and separate with precipitated liquid E;
6., add entry among the precipitated liquid E, the weight ratio of precipitated liquid E and water is 1: 7-10, this liquid is transferred pH to 7-8, and stir under the 40-50 ℃ of condition, natural subsidence obtains albumin soln G and precipitated liquid R, will go up albumin soln G and separate with precipitated liquid R;
7., precipitated liquid R is filtered by filter, obtain protein solution I; Protein solution C, protein solution D, protein solution G, protein solution I are mixed, and separate, collect parting liquid J, and transfer pH to 5.5-6.5 by 6000 rev/mins separating machine;
8., parting liquid J is passed through ultra-filtration membrane, recovery solid substance and filtrate K;
9., above-mentioned solid substance obtains soybean protein isolate through flash distillation, sterilization, spraying drying;
(2), extract soybean isoflavones
1., above-mentioned filtrate K is put in the stirred pot, transfer pH8-11, leave standstill;
2., transfer pH4-5, separate with the high speed separator more than 6000 rev/mins, obtain supernatant liquor L and the M that wads a quilt with cotton with fixed attention;
3., the M that will wad a quilt with cotton with fixed attention washing, sterilization, spraying, drying obtains soybean isoflavones;
(3), extract soybean oligosaccharide
1., above-mentioned supernatant liquor L is carried out electrodialysis, make its specific conductivity reach 10 μ s/cm
2Below, be feed liquid Z;
2., with feed liquid Z by macroporous adsorbent resin, obtain by the material N of absorption with macroporous adsorbent resin and effusive feed liquid O;
3., feed liquid O is through concentrating, sterilization, spraying, drying obtains soybean oligosaccharide;
(4), extract Soyasaponin
1., the ethanol with 50-60% will be obtained elutriant P by material N wash-out on macroporous adsorbent resin of absorption with macroporous adsorbent resin;
2., elutriant P after reclaiming ethanol, it is below 5% that water is transferred rare solid content, and by the strong basicity macroporous resin, obtains feed liquid Q;
3., feed liquid Q is concentrated, sterilization, spraying, drying obtains Soyasaponin.
The present invention is owing to adopted above-mentioned design, can continuously the soybean protein isolate in the raw material, soybean isoflavones, soybean oligosaccharide and Soyasaponin be separated, obtain four kinds of products, and the quality of four kinds of products and net content all occupy first place in the world, overcome use single method technology fully, produced the wasting of resources that single variety caused.The inventive method is simple, and required equipment is the common unit of soybean deep processing industry, is highly susceptible to promoting, and has broad application prospects, and for reducing product cost, it is significant to improve the market competitiveness.
The invention will be further described below in conjunction with specific embodiment.
It is as follows that the present invention extracts the method steps of soybean protein isolate, soybean isoflavones, soybean oligosaccharide and Soyasaponin continuously:
(1), adopt the method for continuous extraction to extract soybean protein isolate
1., raw material of the present invention is that (soybean plumular axis is disallowable when immersion oil for defatted soybean meal behind the immersion oil and the soybean plumular axis of pulverizing, because of being rich in soybean isoflavones and Soyasaponin in the soybean plumular axis, for avoiding waste, collect and be used), after adding the soybean plumular axis that collects through pulverizing in the defatted soybean meal, mix;
2., raw material is carried out the lixiviate first time, put in the multi-functional lixiviate stirred pot after adding entry in the raw material, the weight ratio of raw material and water is 1: 13 (weight ratio of raw material and water is 1: between the 10-15 all can);
3., the lixiviate raw material in the multi-functional lixiviate stirred pot is transferred to pH7.5 (pH gets final product) with sodium hydroxide between 7-8, stir 25 minutes (between 20-30 minute all can) under 45 ℃ of (getting final product between 40-50 ℃) conditions, natural subsidence, time approximately needs 20-30 minute, obtain supernatant A and precipitated liquid B, supernatant A is separated with precipitated liquid B;
4., supernatant A is fallen suspended substance by the filter filtering separation, obtain being rich in the protein solution C of soybean protein isolate;
5., precipitated liquid B is carried out the lixiviate second time, its way is, in precipitated liquid B, add entry, the weight ratio of precipitated liquid B and water is 1: 9 (weight ratio of precipitated liquid B and water is 1: between the 7-10 all can), this liquid is transferred to pH7.5 (pH gets final product) with sodium hydroxide between 7-8, stirred natural subsidence 20-30 minute 20 minutes under 45 ℃ of (between 40-50 ℃ all can) conditions, obtain supernatant liquor D and precipitated liquid E, supernatant liquor D is separated with precipitated liquid E;
6., precipitated liquid E is carried out lixiviate for the third time, its way is, in precipitated liquid E, add entry, the weight ratio of precipitated liquid E and water is 1: 9 (weight ratio of precipitated liquid E and water is 1: between the 7-10 all can), this liquid is transferred to pH7.5 (pH gets final product) with sodium hydroxide between 7-8, stirred natural subsidence 20-30 minute 20 minutes under 45 ℃ of (between 40-50 ℃ all can) conditions, obtain plain white G and precipitated liquid R, will go up plain white G and separate with precipitated liquid R;
7., precipitated liquid R is filtered by filter, obtain protein solution I and filter cake, filter cake can be transported to expanded workshop and supply raw materials for producing puffed food; Protein solution C, protein solution D, protein solution G, protein solution I are mixed, and separate the fine-fibered of removing in the protein liquid, collect protein isolate liquid J, and transfer pH to 6 (pH gets final product) between 5.5-6.5 by 6000 rev/mins separating machine;
8., be 0.1-1.0Mpa with protein isolate liquid J by pressure reduction, the aperture of film is the ultra-filtration membrane of 50-100nm, reclaims solid substance and filtrate K;
9., after above-mentioned solid substance adds water and be transferred to concentration and be 12-16%, obtain soybean protein isolate, the net content of soybean protein isolate is more than 94% in this product, and the product yield is more than 40% through the flash distillation of high temperature flasher, sterilization, spraying drying;
(2), extract soybean isoflavones
1., above-mentioned filtrate K is put in the stirred pot, transfer pH9 (pH between 8-11 all can), left standstill 30 minutes;
2., transfer the pH to 4.5 (pH gets final product) of above-mentioned solution between 4-5, soybean isoflavones is separated out, separate, obtain supernatant liquor L and the M that wads a quilt with cotton with fixed attention with the high speed separator more than 6000 rev/mins with HCl;
3., the M that will wad a quilt with cotton with fixed attention washing twice, wad a quilt with cotton the with fixed attention method of M of washing is will wash the M that wads a quilt with cotton with fixed attention and place water washing tank, add entry, the weight ratio of washing wad a quilt with cotton with fixed attention M and water is more than 1: 15, after stirring, separate with the separating machine more than 6000 rev/mins, collect and coagulate wadding, carry out the washing second time then, the soybean isoflavones that obtains adds soft water adjustment solid content and reaches the spraying drying standard, sterilization, spraying, drying, obtain soybean isoflavones, the net content of soybean isoflavones is more than 85% in the products obtained therefrom;
(3), extract soybean oligosaccharide
1., above-mentioned supernatant liquor L is carried out electrodialysis, remove each metal ion species and salts substances, make its specific conductivity reach 10 μ s/cm
2Below, be feed liquid Z;
2., feed liquid Z is passed through nonpolarity macroporous adsorptive resins chromatographic column (as the HP-20 resin, D370 resin etc.) adsorpting pigment and phenolic hydroxyl group compound, the exchange volume ratio of feed liquid and resin is 50: 1 (volume ratio of feed liquid and resin is 45～55: 1 gets final product), preserves the material N and the effusive feed liquid O of resin absorption;
3., feed liquid O is through reverse osmosis concentration (solid substance should be not less than 10% after concentrating), sterilization (because the oligose moisture absorption very easily when dry, so should add the low heat value additives such as dextrin of suitable proportion before the sterilization, the weight ratio that adds dextrin and raw material is, raw material: dextrin=95: 1), spraying, drying, obtain soybean oligosaccharide, the net content of soybean oligosaccharide is more than 60% in the products obtained therefrom;
(4), extract Soyasaponin
1., the ethanol of (between the 50-60% all can) will be obtained elutriant P by material N wash-out on macroporous adsorbent resin of absorption with macroporous adsorbent resin with 55%;
2., after vacuum concentration reclaimed ethanol, it was below 5% that water is transferred rare solid content to elutriant P, and removes impurity such as pigment by strong basicity macroporous resin (as resin 201, resin 280), obtains feed liquid Q in vacuum concentration pot;
3., feed liquid Q is concentrated (, making the content of solid substance satisfy the condition of spraying) with the method for reverse osmosis or vacuum concentration, sterilization, spraying, drying obtains Soyasaponin, the net content of Soyasaponin is more than 60% in the products obtained therefrom.
Claims (7)
1, the method for a kind of continuous extraction soybean protein isolate, soybean isoflavones, soybean oligosaccharide and Soyasaponin is characterized in that: consist essentially of following steps,
(1), extracts soybean protein isolate
1., raw material: the soybean plumular axis of defatted soybean meal and pulverizing;
2., put in the multi-functional lixiviate stirred pot after adding entry in the raw material, the weight ratio of raw material and water is 1: 10-15;
3., the lixiviate raw material in the multi-functional lixiviate stirred pot is transferred to pH7-8, stir under the 40-50 ℃ of condition, natural subsidence obtains supernatant A and precipitated liquid B, and supernatant A is separated with precipitated liquid B;
4., supernatant A is filtered by filter, obtain protein solution C;
5., add entry among the precipitated liquid B, the weight ratio of precipitated liquid B and water is 1: 7-10, this liquid is transferred pH to 7-8, and stir under the 40-50 ℃ of condition, natural subsidence obtains albumin soln D and precipitated liquid E, will go up albumin soln D and separate with precipitated liquid E;
6., add entry among the precipitated liquid E, the weight ratio of precipitated liquid E and water is 1: 7-10, this liquid is transferred pH to 7-8, and stir under the 40-50 ℃ of condition, natural subsidence obtains albumin soln G and precipitated liquid R, will go up albumin soln G and separate with precipitated liquid R;
7., precipitated liquid R is filtered by filter, obtain protein solution I; Protein solution C, protein solution D, protein solution G, protein solution I are mixed, and separate, collect parting liquid J, and transfer pH to 5.5-6.5 by 6000 rev/mins separating machine;
8., parting liquid J is passed through ultra-filtration membrane, recovery solid substance and filtrate K;
9., above-mentioned solid substance obtains soybean protein isolate through flash distillation, sterilization, spraying drying;
(2), extract soybean isoflavones
1., above-mentioned filtrate K is put in the stirred pot, transfer pH8-11, leave standstill;
2., transfer pH4-5, separate with the high speed separator more than 6000 rev/mins, obtain supernatant liquor L and the M that wads a quilt with cotton with fixed attention;
3., the M that will wad a quilt with cotton with fixed attention washing, sterilization, spraying, drying obtains soybean isoflavones;
(3), extract soybean oligosaccharide
1., above-mentioned supernatant liquor L is carried out electrodialysis, make its specific conductivity reach 10 μ s/cm
2Below, be feed liquid Z;
2., with feed liquid Z by macroporous adsorbent resin, obtain by the material N of absorption with macroporous adsorbent resin and effusive feed liquid O;
3., feed liquid O is through concentrating, sterilization, spraying, drying obtains soybean oligosaccharide;
(4), extract Soyasaponin
1., the ethanol with 50-60% will be obtained elutriant P by material N wash-out on macroporous adsorbent resin of absorption with macroporous adsorbent resin;
2., elutriant P after reclaiming ethanol, it is below 5% that water is transferred rare solid content, and by the strong basicity macroporous resin, obtains feed liquid Q;
3., feed liquid Q is concentrated, sterilization, spraying, drying obtains Soyasaponin.
2, the method for a kind of continuous extraction soybean protein isolate according to claim 1, soybean isoflavones, soybean oligosaccharide and Soyasaponin is characterized in that: described (1), extract in the soybean protein isolate 3., 5., regulate pH sodium hydroxide in 6.; The time of stirring under 40-50 ℃ of condition 3. is 20-30 minute; 3. the static balance time in is 20-30 minute; 8. its pressure reduction of ultra-filtration membrane that middle parting liquid J passes through is 0.1-1.0Mpa, and the aperture of film is 50-100nm.
3, the method for a kind of continuous extraction soybean protein isolate according to claim 1, soybean isoflavones, soybean oligosaccharide and Soyasaponin is characterized in that: described (2), the time of extracting in the soybean isoflavones 1., leaving standstill are 30 minutes; 2. use the HCl adjust pH in; 3. in, twice of the M that will wad a quilt with cotton with fixed attention washing.
4, according to the method for claim 1 or 3 described a kind of continuous extraction soybean protein isolates, soybean isoflavones, soybean oligosaccharide and Soyasaponin, it is characterized in that: in described (2), the extraction soybean isoflavones, wad a quilt with cotton the with fixed attention method of M of described washing is, to wash the M that wads a quilt with cotton with fixed attention and place water washing tank, add entry, the weight ratio of washing wad a quilt with cotton with fixed attention M and water is more than 1: 15, after stirring, separate with the separating machine more than 6000 rev/mins, collect and coagulate wadding.
5, the method for a kind of continuous extraction soybean protein isolate according to claim 1, soybean isoflavones, soybean oligosaccharide and Soyasaponin is characterized in that: described (3), extract in the soybean oligosaccharide 3., the content of feed liquid O solid substance after concentrated is more than 10%; Add dextrin before the sterilization.
6, the method for a kind of continuous extraction soybean protein isolate according to claim 5, soybean isoflavones, soybean oligosaccharide and Soyasaponin is characterized in that: during described adding dextrin feed liquid with add dextrin weight ratio be 93-97: 1.
7, the method for a kind of continuous extraction soybean protein isolate according to claim 1, soybean isoflavones, soybean oligosaccharide and Soyasaponin is characterized in that: described (4), extract in the Soyasaponin 2., elutriant P reclaims ethanol through vacuum concentration.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100345837C (en) * | 2005-01-31 | 2007-10-31 | 重庆工学院 | Extraction of soya isoflavone, concentrated soya protein and soya oligose |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1293068C (en) * | 2002-09-28 | 2007-01-03 | 胡传璞 | Soybean extract with health-care function , its preparing process and use |
| CN101283746B (en) * | 2008-05-14 | 2011-07-27 | 逄镜萍 | Process for extracting the soyabean oligosaccharides by air-float purification and film integration |
| CN101463073B (en) * | 2009-01-08 | 2011-08-31 | 中国科学院西双版纳热带植物园 | Continuous extraction method for Jatrohpa curcas toxic protein |
| CN102617702A (en) * | 2012-03-09 | 2012-08-01 | 姜浩奎 | Production method of peptidoglycan |
| CN103947821B (en) * | 2014-05-16 | 2016-03-23 | 山东万得福实业集团有限公司 | A kind of production technology of bean curd bag specialized soybean albumen and using method |
| CN105454622A (en) * | 2015-11-16 | 2016-04-06 | 宿州市皖神面制品有限公司 | Production method of pea protein powder |
| CN107348094A (en) * | 2017-08-30 | 2017-11-17 | 山东禹王生态食业有限公司 | A kind of production method of soybean protein isolate |
| CN110128387B (en) * | 2019-06-17 | 2021-05-11 | 山东师范大学 | Method for extracting soybean isoflavone aglycone |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN100345837C (en) * | 2005-01-31 | 2007-10-31 | 重庆工学院 | Extraction of soya isoflavone, concentrated soya protein and soya oligose |
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