CN102617702A - Production method of peptidoglycan - Google Patents
Production method of peptidoglycan Download PDFInfo
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- CN102617702A CN102617702A CN2012100620240A CN201210062024A CN102617702A CN 102617702 A CN102617702 A CN 102617702A CN 2012100620240 A CN2012100620240 A CN 2012100620240A CN 201210062024 A CN201210062024 A CN 201210062024A CN 102617702 A CN102617702 A CN 102617702A
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Abstract
The invention relates to a production method of peptidoglycan, and mainly solves the technical problems that: in the prior art, the production process is complicated, the cost is high, and the peptide tastes bad and is inconvenient to eat. The production method of the peptidoglyca comprises the following steps that: degreased cryogenic meal is used as a raw material to extract peptidoglycan; and protein isolate and oligosaccharide can also be used as raw materials to extract the peptidoglycan. The weight ratio of feed to water is 1:15, the pH value of a feed liquid is 9-11, and the raw materials are fully stirred for more than 5 hours at a temperature of 50 DEG C to 55 DEG C, are subjected to acid precipitation and water wash to purify protein, are separated by a decanter, and are subjected to electrodialysis, decolorization column purification, reverse osmosis concentration, activated carbon treatment and final spray drying to obtain a peptidoglycan product. The production method of the peptidoglycan has the characteristics that the preparation method is simple, the production cost of the product is low, the product is convenient to eat, has no bitterness, and tastes good. The problem that the peptide tastes unpleasant is solved, and the technical problem that the efficacy of peptide and oligosaccharide products embodies in one product is solved. The method has a good promotion prospect.
Description
Technical field
The present invention relates to a kind of Polysaccharides, peptide complexes working method, exactly is a kind of with peptide and oligose polymeric novel method, belongs to the soybean deep processing technology field.
Background technology
In recent years, the technology of soybean deep processing is constantly improving, and the peptide that wherein in soybean, prepares has the raising body immunity, strengthens physical efficiency, and is obvious to the human body cell repairing effect, is a kind of true solution, absorbs easily.Though the nutritive ingredient of peptide is generally acknowledged that by people because peptide has bitter taste, its mouthfeel is poor, the promotion and application of peptide have therefore been influenced.Oligose is the extract in the soybean, has the two-way effect of conditioning diarrhoea and constipation, also can improve looks.People when edible, need respectively that peptide is edible, pretty troublesome, inconvenient with the oligose bath, and the complex manufacturing of product, cost high, produce the sewage pollution environment that discharges.
Summary of the invention
The objective of the invention is the deficiency to above-mentioned prior art, a kind of preparation method is simple, production cost is low and provide, instant, and the Polysaccharides, peptide complexes working method that after peptide and the oligose polymerization nutritive ingredient is enhanced.
The present invention can be a raw material with the degreasing low temperature dregs of rice, extracts Polysaccharides, peptide complexes; Can be that raw material extracts Polysaccharides, peptide complexes also with protein isolates and oligose.
One, be raw material with the degreasing low temperature dregs of rice, the working method of extracting Polysaccharides, peptide complexes realizes through following process step:
A, be raw material with the degreasing low temperature dregs of rice; Material water is by weight being 1: 15, and material liquid pH value 9-11 fully stirs more than 5 hours under 50 ℃ of-55 ℃ of conditions of temperature; Effective constituent is fully leached; The feed liquid that leaches is through the horizontal helical type separating machine, and rotating speed is in the zone of 3500-4500r/min, with mixing solutions such as water soluble oligosaccharide, albumen, NOVASOY 400 and saponin and undissolved robust fibre separately.
B, the heavy albumen of acid: the water-soluble mixed solution acid after the step a separation is heavy; Adjust pH 4.45 ± 0.2; Separate through the horizontal helical type separating machine, rotating speed separates albumen and mixing solutionss such as water soluble oligosaccharide, saponin, NOVASOY 400 in the zone of 3500-4500r/min.
C, washing albumen: material water is by weight 1: 70, material liquid pH value 9.5-11, and 50 ℃-55 ℃ of temperature are washed and albumen were all dissolved in 2 hours, recall to pH value 4.45 ± 0.2 more again, separate with the horizontal helical type separating machine once more.
D, enzymolysis process: what enzyme used is the Sumizyme MP that Wuxi snow Mei Zhijichang produces; Concentration of substrate 3.5%, 55 ℃-60 ℃ of temperature, pH value 7.5-11; Enzyme concentration is the 0.5-2.0% of albumen solubility rate; Enzymolysis after inactivation finishes, is purified through Plate Filtration, electrodialysis, resin column more than 6 hours successively.
E, with the water-soluble mixed solution after the heavy albumen sepn of acid among the step b, be made into the 1.5%-1.8% solid substance, pH value 3.0 is successively through Plate Filtration, electrodialysis, resin column purification.
Feed liquid after f, the purification is respectively through reverse osmosis concentration, peptide liquid solid substance 8%-10%, pH value 4.1-4.4, oligose liquid solid substance 8%-10%, pH value 4.1-4.4.
G, peptide liquid concentration are puddled together in the 40%-20% ratio at 60%-80% and oligomeric sugar concentration, use activated carbon decolorizing, 70 ℃ of temperature; The gac addition is the 1.5%-2.0% of feed liquid weight; Stirred 1 hour, through Plate Filtration, the direct spraying drying of filtrating; Promptly get the Polysaccharides, peptide complexes product, with said product sterilising packaging.
Two, be raw material with protein isolates and oligose, the working method of extracting Polysaccharides, peptide complexes realizes through following process step:
A, washing protein isolates: material water is by weight 1: 70, material liquid pH value 9.5-11, and 50 ℃-55 ℃ of temperature make albumen all after the dissolving, and adjust pH 4.45 ± 0.2 separates through the horizontal helical type separating machine, and rotating speed is in the zone of 3500-4500r/min.
B, enzymolysis process: what enzyme used is the Sumizyme MP that Wuxi snow Mei Zhijichang produces; It is 3.5% that albumen after the washing is made into concentration of substrate, 55 ℃-60 ℃ of temperature, pH value 7.5-11; Enzyme concentration is the 0.5-2.0% of protein isolates weight; Enzymolysis after inactivation finishes, is purified through Plate Filtration, electrodialysis, resin column more than 6 hours successively.
The supernatant of c, production protein concentrate is as the raw material of oligose, and solid substance is made into 1.5-2.0%, and pH value 3.0 is purified through Plate Filtration, electrodialysis, resin column successively.
D, the feed liquid after will purifying are respectively through reverse osmosis concentration, peptide liquid solid substance 8%-10%, pH value 4.1-4.4, oligose liquid solid substance 8%-10%, pH value 4.1-4.4.
E, peptide liquid concentration are puddled together in the 40%-20% ratio at 60%-80% and oligomeric sugar concentration, use activated carbon decolorizing, 70 ℃ of temperature; The gac addition is the 1.5%-2.0% of feed liquid weight; Stirred 1 hour, through Plate Filtration, the direct spraying drying of filtrating; Promptly get the Polysaccharides, peptide complexes product, with said product sterilising packaging.
Characteristics of the present invention are: the preparation method is simple, the products production cost is low, instant, and no bitter taste, mouthfeel are good.When having solved the unhappy problem of peptide mouthfeel, solved two kinds of product efficacy of peptide and oligose again and be embodied in the technical problem on a kind of product.Present method has the excellent popularization prospect.
Embodiment
Embodiment one
With the degreasing low temperature dregs of rice is raw material, and the working method of extracting Polysaccharides, peptide complexes realizes through following process step:
A, be raw material with the degreasing low temperature dregs of rice; Material water is by weight being 1: 15, and material liquid pH value 9-11 fully stirs more than 5 hours under 50 ℃ of-55 ℃ of conditions of temperature; Effective constituent is fully leached; The feed liquid that leaches is through the horizontal helical type separating machine, and rotating speed is in the zone of 3500-4500r/min, with mixing solutions such as water soluble oligosaccharide, albumen, NOVASOY 400 and saponin and undissolved robust fibre separately.
B, the heavy albumen of acid: the depth of water property mixed solution acid after the step a separation is heavy; Adjust pH 4.45 ± 0.2 separates through the horizontal helical type separating machine; Rotating speed is in the zone of 3500-4500r/min, with mixing solutionss such as albumen and water soluble oligosaccharide, saponin, NOVASOY 400 separately.
C, washing albumen: expect water by weight 1: 70, material liquid pH value 9.5-11, temperature is washed till 2 hours for 50 ℃-55 ℃ all dissolves albumen, recalls to pH value 4.45 ± 0.2 more again, separates with the horizontal helical type separating machine once more.
D, enzymolysis process: what enzyme used is the Sumizyme MP that Wuxi snow Mei Zhijichang produces; Concentration of substrate 3.5%, 55 ℃-60 ℃ of temperature, pH value 7.5-11; Enzyme concentration is the 0.5-2.0% of albumen solubility rate; Enzymolysis after inactivation finishes, is purified through Plate Filtration, electrodialysis, resin column more than 6 hours successively.
E, with the water-soluble mixed solution after the heavy albumen sepn of acid among the step b, be made into the 1.5%-1.8% solid substance, pH value 3.0 is successively through Plate Filtration, electrodialysis, resin column purification.
Feed liquid after f, the purification is respectively through reverse osmosis concentration, peptide liquid solid substance 8%-10%, pH value 4.1-4.4, oligose liquid solid substance 8%-10%, pH value 4.1-4.4.
G, peptide liquid concentration are puddled together in the 40%-20% ratio at 60%-80% and oligomeric sugar concentration, use activated carbon decolorizing, 70 ℃ of temperature; The gac addition is the 1.5%-2.0% of feed liquid weight; Stirred 1 hour, through Plate Filtration, the direct spraying drying of filtrating; Promptly get the Polysaccharides, peptide complexes product, with said product sterilising packaging.
Embodiment two
With protein isolates and oligose is the working method that raw material extracts Polysaccharides, peptide complexes:
A, washing protein isolates: material water is by weight 1: 70, material liquid pH value 9.5-11, and 50 ℃-55 ℃ of temperature make albumen all after the dissolving, and adjust pH 4.45 ± 0.2 separates through the horizontal helical type separating machine, and rotating speed is in the zone of 3500-4500r/min.
B, enzymolysis process: what enzyme used is the Sumizyme MP that Wuxi snow Mei Zhijichang produces; It is 3.5% that albumen after the washing is made into concentration of substrate, 55 ℃-60 ℃ of temperature, pH value 7.5-11; Enzyme concentration is the 0.5-2.0% of protein isolates weight; Enzymolysis after inactivation finishes, is purified through Plate Filtration, electrodialysis, resin column more than 6 hours successively.
The supernatant of c, production protein concentrate is as the raw material of oligose, and solid substance is made into 1.5-2.0%, and pH value 3.0 is purified through Plate Filtration, electrodialysis, resin column successively.
D, the feed liquid after will purifying are respectively through reverse osmosis concentration, peptide liquid solid substance 8%-10%, pH value 4.1-4.4, oligose liquid solid substance 8%-10%, pH value 4.1-4.4.
E, peptide liquid concentration are puddled together in the 40%-20% ratio at 60%-80% and oligomeric sugar concentration, use activated carbon decolorizing, 70 ℃ of temperature; The gac addition is the 1.5%-2.0% of feed liquid weight; Stirred 1 hour, through Plate Filtration, the direct spraying drying of filtrating; Promptly get the Polysaccharides, peptide complexes product, with said product sterilising packaging.
Claims (2)
1. Polysaccharides, peptide complexes working method, this method is a raw material with the degreasing low temperature dregs of rice, extracts Polysaccharides, peptide complexes, concrete steps are described below:
A, be raw material with the degreasing low temperature dregs of rice; Material water is by weight being 1: 15, and material liquid pH value 9-11 fully stirs more than 5 hours under 50 ℃ of-55 ℃ of conditions of temperature; Effective constituent is fully leached; The feed liquid that leaches is through the horizontal helical type separating machine, and rotating speed is in the zone of 3500-4500r/min, with mixing solutions such as water soluble oligosaccharide, albumen, NOVASOY 400 and saponin and undissolved robust fibre separately;
B, the heavy albumen of acid: the water-soluble mixed solution acid after the step a separation is heavy; Adjust pH 4.45 ± 0.2; Separate through the horizontal helical type separating machine, rotating speed separates albumen and mixing solutionss such as water soluble oligosaccharide, saponin, NOVASOY 400 in the zone of 3500-4500r/min;
C, washing albumen: material water is by weight 1: 70, material liquid pH value 9.5-11, and 50 ℃-55 ℃ of temperature are washed and albumen were all dissolved in 2 hours, recall to pH value 4.45 ± 0.2 more again, separate with the horizontal helical type separating machine once more;
D, enzymolysis process: what enzyme used is the Sumizyme MP that Wuxi snow Mei Zhijichang produces; Concentration of substrate 3.5%, 55 ℃-60 ℃ of temperature, pH value 7.5-11; Enzyme concentration is the 0.5-2.0% of albumen solubility rate; Enzymolysis after inactivation finishes, is purified through Plate Filtration, electrodialysis, resin column more than 6 hours successively;
E, with the water-soluble mixed solution after the heavy albumen sepn of acid among the step b, be made into the 1.5%-1.8% solid substance, pH value 3.0 is successively through Plate Filtration, electrodialysis, resin column purification;
Feed liquid after f, the purification is respectively through reverse osmosis concentration, peptide liquid solid substance 8%-10%, pH value 4.1-4.4, oligose liquid solid substance 8%-10%, pH value 4.1-4.4;
G, peptide liquid concentration are puddled together in the 40%-20% ratio at 60%-80% and oligomeric sugar concentration, use activated carbon decolorizing, 70 ℃ of temperature; The gac addition is the 1.5%-2.0% of feed liquid weight; Stirred 1 hour, through Plate Filtration, the direct spraying drying of filtrating; Promptly get the Polysaccharides, peptide complexes product, with said product sterilising packaging.
2. Polysaccharides, peptide complexes working method, this method is a raw material with protein isolates and oligose, extracts Polysaccharides, peptide complexes, concrete steps are described below:
A, washing protein isolates: material water is by weight 1: 70, material liquid pH value 9.5-11, and 50 ℃-55 ℃ of temperature make albumen all after the dissolving, and adjust pH 4.45 ± 0.2 separates through the horizontal helical type separating machine, and rotating speed is in the zone of 3500-4500r/min;
B, enzymolysis process: what enzyme used is the Sumizyme MP that Wuxi snow Mei Zhijichang produces; It is 3.5% that albumen after the washing is made into concentration of substrate, 55 ℃-60 ℃ of temperature, pH value 7.5-11; Enzyme concentration is the 0.5-2.0% of protein isolates weight; Enzymolysis after inactivation finishes, is purified through Plate Filtration, electrodialysis, resin column more than 6 hours successively;
The supernatant of c, production protein concentrate is as the raw material of oligose, and solid substance is made into 1.5-2.0%, and pH value 3.0 is purified through Plate Filtration, electrodialysis, resin column successively;
D, the feed liquid after will purifying are respectively through reverse osmosis concentration, peptide liquid solid substance 8%-10%, pH value 4.1-4.4, oligose liquid solid substance 8%-10%, pH value 4.1-4.4;
E, peptide liquid concentration are puddled together in the 40%-20% ratio at 60%-80% and oligomeric sugar concentration, use activated carbon decolorizing, 70 ℃ of temperature; The gac addition is the 1.5%-2.0% of feed liquid weight; Stirred 1 hour, through Plate Filtration, the direct spraying drying of filtrating; Promptly get the Polysaccharides, peptide complexes product, with said product sterilising packaging.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012100620240A CN102617702A (en) | 2012-03-09 | 2012-03-09 | Production method of peptidoglycan |
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| CN2012100620240A CN102617702A (en) | 2012-03-09 | 2012-03-09 | Production method of peptidoglycan |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103113481A (en) * | 2012-08-16 | 2013-05-22 | 中南民族大学 | Mutant strain ZN0871v11 cell wall peptidoglycan and preparation method thereof |
| CN105907824A (en) * | 2016-05-10 | 2016-08-31 | 江苏巨托生物科技有限公司 | Method for producing glycopeptide by taking edible soybean meal as raw material |
| CN109619264A (en) * | 2018-10-29 | 2019-04-16 | 长春大学 | The clean preparation method of the compound water-soluble function factor of soybean probiotic peptide |
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| JPH04183371A (en) * | 1990-11-15 | 1992-06-30 | Snow Brand Milk Prod Co Ltd | Bone-fortifying food, feed and pharmaceutical |
| CN1282525A (en) * | 1999-08-03 | 2001-02-07 | 姜浩奎 | Process for extracting soybean protein |
| CN1323534A (en) * | 2000-05-15 | 2001-11-28 | 姜浩奎 | Continuous technolgical process of extracting soybean and separating protein, isoflavone, oligosaccharide and saponin |
| CN1327983A (en) * | 2000-06-08 | 2001-12-26 | 姜浩奎 | Process for continuously extracting glycitin, olegose and saponin |
| CN1329103A (en) * | 2001-08-07 | 2002-01-02 | 姜浩奎 | Method for extracting protein, short peptide, nucleic acid, isoflavone, saponin and oligosaccharide by using high and low temperature soybean cake |
| CN1425323A (en) * | 2003-01-09 | 2003-06-25 | 姜浩奎 | Method for extracting composite soy bean function factor from high and low temperature degreased bean dregs |
| CN1488274A (en) * | 2002-10-10 | 2004-04-14 | 吉林省高等院校科技开发研究中心 | Method for producing soybean protein which content is not less than 60% useing high-low temperature bean dregs as raw material |
| CN1522596A (en) * | 2003-02-17 | 2004-08-25 | 吉林省高等院校科技开发研究中心 | Method for extracting composite corn embryo health care functional factor having corn embryo cake as raw material |
| CN1537865A (en) * | 2003-10-23 | 2004-10-20 | 姜浩奎 | Method of extracting lactoalbumin, nucleic acid oligosaccharide, isoflavone, saponin from yellow waste water generated by producing soybean |
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2012
- 2012-03-09 CN CN2012100620240A patent/CN102617702A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH04183371A (en) * | 1990-11-15 | 1992-06-30 | Snow Brand Milk Prod Co Ltd | Bone-fortifying food, feed and pharmaceutical |
| CN1282525A (en) * | 1999-08-03 | 2001-02-07 | 姜浩奎 | Process for extracting soybean protein |
| CN1323534A (en) * | 2000-05-15 | 2001-11-28 | 姜浩奎 | Continuous technolgical process of extracting soybean and separating protein, isoflavone, oligosaccharide and saponin |
| CN1327983A (en) * | 2000-06-08 | 2001-12-26 | 姜浩奎 | Process for continuously extracting glycitin, olegose and saponin |
| CN1329103A (en) * | 2001-08-07 | 2002-01-02 | 姜浩奎 | Method for extracting protein, short peptide, nucleic acid, isoflavone, saponin and oligosaccharide by using high and low temperature soybean cake |
| CN1488274A (en) * | 2002-10-10 | 2004-04-14 | 吉林省高等院校科技开发研究中心 | Method for producing soybean protein which content is not less than 60% useing high-low temperature bean dregs as raw material |
| CN1425323A (en) * | 2003-01-09 | 2003-06-25 | 姜浩奎 | Method for extracting composite soy bean function factor from high and low temperature degreased bean dregs |
| CN1522596A (en) * | 2003-02-17 | 2004-08-25 | 吉林省高等院校科技开发研究中心 | Method for extracting composite corn embryo health care functional factor having corn embryo cake as raw material |
| CN1537865A (en) * | 2003-10-23 | 2004-10-20 | 姜浩奎 | Method of extracting lactoalbumin, nucleic acid oligosaccharide, isoflavone, saponin from yellow waste water generated by producing soybean |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103113481A (en) * | 2012-08-16 | 2013-05-22 | 中南民族大学 | Mutant strain ZN0871v11 cell wall peptidoglycan and preparation method thereof |
| CN103113481B (en) * | 2012-08-16 | 2014-12-10 | 中南民族大学 | Mutant strain ZN0871v11 cell wall peptidoglycan and preparation method thereof |
| CN105907824A (en) * | 2016-05-10 | 2016-08-31 | 江苏巨托生物科技有限公司 | Method for producing glycopeptide by taking edible soybean meal as raw material |
| CN105907824B (en) * | 2016-05-10 | 2022-03-29 | 江苏巨托生物科技有限公司 | Method for producing glycopeptide by taking edible soybean meal as raw material |
| CN109619264A (en) * | 2018-10-29 | 2019-04-16 | 长春大学 | The clean preparation method of the compound water-soluble function factor of soybean probiotic peptide |
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