CN113755616B - 耐药性金黄色葡萄球菌MecA和ErmA基因的多重荧光RPA检测方法及试剂盒 - Google Patents
耐药性金黄色葡萄球菌MecA和ErmA基因的多重荧光RPA检测方法及试剂盒 Download PDFInfo
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- CN113755616B CN113755616B CN202111131105.7A CN202111131105A CN113755616B CN 113755616 B CN113755616 B CN 113755616B CN 202111131105 A CN202111131105 A CN 202111131105A CN 113755616 B CN113755616 B CN 113755616B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
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Abstract
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CN202111131105.7A CN113755616B (zh) | 2021-09-26 | 2021-09-26 | 耐药性金黄色葡萄球菌MecA和ErmA基因的多重荧光RPA检测方法及试剂盒 |
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Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103421906A (zh) * | 2013-08-23 | 2013-12-04 | 中国检验检疫科学研究院 | 检测金黄色葡萄球菌耐药性的组合物和方法 |
CN105567866A (zh) * | 2016-03-25 | 2016-05-11 | 苏州达麦迪生物医学科技有限公司 | 用于耐甲氧西林金黄色葡萄球菌检测的引物、试剂盒及方法 |
CN106119403A (zh) * | 2016-08-30 | 2016-11-16 | 孙青菊 | 一组用于金黄色葡萄球菌多重耐药基因检测的液态芯片引物和探针 |
CN106222248A (zh) * | 2016-07-08 | 2016-12-14 | 宁波基内生物技术有限公司 | 一种检测耐甲氧西林金黄色葡萄球菌耐药性基因的引物、探针、方法及试剂盒 |
Family Cites Families (1)
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WO2019178188A1 (en) * | 2018-03-13 | 2019-09-19 | Microinvestigate, Llc | Direct-to-consumer genomic diagnostic device |
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Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103421906A (zh) * | 2013-08-23 | 2013-12-04 | 中国检验检疫科学研究院 | 检测金黄色葡萄球菌耐药性的组合物和方法 |
CN105567866A (zh) * | 2016-03-25 | 2016-05-11 | 苏州达麦迪生物医学科技有限公司 | 用于耐甲氧西林金黄色葡萄球菌检测的引物、试剂盒及方法 |
CN106222248A (zh) * | 2016-07-08 | 2016-12-14 | 宁波基内生物技术有限公司 | 一种检测耐甲氧西林金黄色葡萄球菌耐药性基因的引物、探针、方法及试剂盒 |
CN106119403A (zh) * | 2016-08-30 | 2016-11-16 | 孙青菊 | 一组用于金黄色葡萄球菌多重耐药基因检测的液态芯片引物和探针 |
Non-Patent Citations (3)
Title |
---|
Sascha Lutz 等.Microfluidic lab-on-a-foil for nucleic acid analysis based on isothermal recombinase polymerase amplification (RPA).Lab Chip ..2010,第10卷(第7期),887-893. * |
Xiaosong Wu 等.MS2 device: smartphone-facilitated mobile nucleic acid analysis on microfluidic device.Analyst..2021,第146卷(第12期),3823-3833. * |
Zhonglin Shi 等.Duplex real-time fluorescent recombinase polymerase amplification for the rapid and sensitive detection of two resistance genes in drug-resistant Staphylococcus aureus.J Microbiol Methods ..2022,第202卷文章号:106590. * |
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