CN113748212B - 用于制备gdp-岩藻糖的酶促方法 - Google Patents

用于制备gdp-岩藻糖的酶促方法 Download PDF

Info

Publication number
CN113748212B
CN113748212B CN202080026075.0A CN202080026075A CN113748212B CN 113748212 B CN113748212 B CN 113748212B CN 202080026075 A CN202080026075 A CN 202080026075A CN 113748212 B CN113748212 B CN 113748212B
Authority
CN
China
Prior art keywords
guanosine
fucose
mannose
gdp
kinase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202080026075.0A
Other languages
English (en)
Chinese (zh)
Other versions
CN113748212A (zh
Inventor
R·马霍尔
T·F·T·莱克瑟
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Original Assignee
Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Max Planck Gesellschaft zur Foerderung der Wissenschaften eV filed Critical Max Planck Gesellschaft zur Foerderung der Wissenschaften eV
Publication of CN113748212A publication Critical patent/CN113748212A/zh
Application granted granted Critical
Publication of CN113748212B publication Critical patent/CN113748212B/zh
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/32—Nucleotides having a condensed ring system containing a six-membered ring having two N-atoms in the same ring, e.g. purine nucleotides, nicotineamide-adenine dinucleotide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/44—Preparation of O-glycosides, e.g. glucosides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/01271—GDP-L-fucose synthase (1.1.1.271)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01002—Glucokinase (2.7.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01052—Fucokinase (2.7.1.52)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01073—Inosine kinase (2.7.1.73)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01162—N-Acetylhexosamine 1-kinase (2.7.1.162)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/04—Phosphotransferases with a phosphate group as acceptor (2.7.4)
    • C12Y207/04001—Polyphosphate kinase (2.7.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07013—Mannose-1-phosphate guanylyltransferase (2.7.7.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07022—Mannose-1-phosphate guanylyltransferase (GDP) (2.7.7.22)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/0703—Fucose-1-phosphate guanylyltransferase (2.7.7.30)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/01—Hydro-lyases (4.2.1)
    • C12Y402/01047—GDP-mannose 4,6-dehydratase (4.2.1.47), i.e. GMD
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y504/00—Intramolecular transferases (5.4)
    • C12Y504/02—Phosphotransferases (phosphomutases) (5.4.2)
    • C12Y504/02008—Phosphomannomutase (5.4.2.8)

Landscapes

  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN202080026075.0A 2019-04-01 2020-03-31 用于制备gdp-岩藻糖的酶促方法 Active CN113748212B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP19166549.6 2019-04-01
EP19166549.6A EP3719135A1 (en) 2019-04-01 2019-04-01 Enzymatic method for preparation of gdp-fucose
PCT/EP2020/059182 WO2020201315A1 (en) 2019-04-01 2020-03-31 Enzymatic method for preparation of gdp-fucose

Publications (2)

Publication Number Publication Date
CN113748212A CN113748212A (zh) 2021-12-03
CN113748212B true CN113748212B (zh) 2023-04-25

Family

ID=66049072

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202080026075.0A Active CN113748212B (zh) 2019-04-01 2020-03-31 用于制备gdp-岩藻糖的酶促方法

Country Status (7)

Country Link
US (2) US11795486B2 (enExample)
EP (2) EP3719135A1 (enExample)
JP (1) JP7299339B2 (enExample)
CN (1) CN113748212B (enExample)
CA (1) CA3133590C (enExample)
ES (1) ES2949962T3 (enExample)
WO (1) WO2020201315A1 (enExample)

Families Citing this family (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2022133213A1 (en) * 2020-12-18 2022-06-23 Debut Biotechnology, Inc. Cell-free production of geranyl pyrophosphate from glycerol in a cell-free manufacturing system
CN114990175B (zh) * 2021-10-22 2023-03-31 岩唐生物科技(杭州)有限责任公司 一种岩藻糖衍生物的合成方法
CN119731313A (zh) * 2023-08-16 2025-03-28 北京国科星联科技有限公司 岩藻糖及其中间体的制备方法及其应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI941732L (fi) * 1991-10-15 1994-06-14 Scripps Research Inst Fukosyloitujen hiilihydraattien valmistaminen sokerinukleotidien entsymaattisella fukosylointisyteesillä ja GDP-fukoosin in situ regenerointi
JPH0937785A (ja) * 1995-05-25 1997-02-10 Ajinomoto Co Inc ヌクレオシド−５’−燐酸エステルの製造法
JP4304727B2 (ja) * 1996-11-21 2009-07-29 味の素株式会社 ヌクレオシド−５’−燐酸エステルの製造法
JP2001112488A (ja) * 1999-08-10 2001-04-24 Kyowa Hakko Kogyo Co Ltd Ｇｄｐ−フコースの製造法
US6875591B1 (en) * 1999-08-10 2005-04-05 Kyowa, Hakko Kogyo Co., Ltd. Process for producing GDP-fucose
JP4192408B2 (ja) * 2000-06-23 2008-12-10 味の素株式会社 ヌクレオシド−５’−燐酸エステルの製造法
FI109811B (fi) * 2000-09-26 2002-10-15 Medicel Oy Menetelmä GDP-L-fukoosin valmistamiseksi ja menetelmässä käytettäviä välineitä
US20020132320A1 (en) 2001-01-10 2002-09-19 Wang Peng George Glycoconjugate synthesis using a pathway-engineered organism
KR101179914B1 (ko) * 2009-02-02 2012-09-06 서울대학교산학협력단 Ｎａｄｐｈ 및 구아노신 누클레오타이드 생합성 효소의 추가 발현에 의한 재조합 대장균에서 ｇｄｐ-푸코즈의 생산방법
CN107805622B (zh) * 2017-11-08 2021-04-30 光明乳业股份有限公司 一种合成鸟苷二磷酸岩藻糖的重组枯草芽孢杆菌及其构建方法与应用

Also Published As

Publication number Publication date
US20240124911A1 (en) 2024-04-18
EP3719135A1 (en) 2020-10-07
US11795486B2 (en) 2023-10-24
EP3784792A1 (en) 2021-03-03
EP3784792B9 (en) 2023-10-04
EP3784792C0 (en) 2023-06-07
CA3133590C (en) 2023-09-12
ES2949962T3 (es) 2023-10-04
CN113748212A (zh) 2021-12-03
JP2022528874A (ja) 2022-06-16
EP3784792B1 (en) 2023-06-07
US20220177940A1 (en) 2022-06-09
WO2020201315A1 (en) 2020-10-08
JP7299339B2 (ja) 2023-06-27
CA3133590A1 (en) 2020-10-08

Similar Documents

Publication Publication Date Title
Ichikawa et al. Enzyme-catalyzed oligosaccharide synthesis
Koeller et al. Synthesis of complex carbohydrates and glycoconjugates: enzyme-based and programmable one-pot strategies
CN114641577B (zh) 用于制备udp-半乳糖的酶方法
US20240124911A1 (en) Enzymatic method for preparation of gdp-fucose
CN113383072B (zh) 酶法生产己糖
CN114630911B (zh) 用于制备UDP-GlcNAc的酶方法
WO2020126613A1 (en) Enzymatic hexosaminidation of lactose
JP3545424B2 (ja) ヌクレオシド５’−トリリン酸の製造法及びその応用
CN115552026A (zh) 用于制备CMP-Neu5Ac的酶促方法
JPWO1998022614A1 (ja) ヌクレオシド５’−トリリン酸の製造法及びその応用
Li et al. Efficient one-pot synthesis of uridine diphosphate galactose employing a trienzyme system
Qian et al. Glycosyltransferases in oligosaccharide synthesis
Hamilton Enzymes in preparative mono-and oligo-saccharide synthesis
HK40048426B (en) Enzymatic method for preparation of gdp-fucose
EP0971035B1 (en) Process for producing uridine diphosphate-n-acetylglucosamine
CA3162349A1 (en) Production of fructose from oligo-/ and/or polysaccharides
CN120989038A (zh) 一种用于合成功能聚糖的多酶催化系统及方法
Frohnmeyer Enzymatic processes for the multigram-scale production of nucleotide sugars

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant