CN113683540A

CN113683540A - Preparation process for extracting antitumor substance sulforaphane from broccoli buds

Info

Publication number: CN113683540A
Application number: CN202110958633.3A
Authority: CN
Inventors: 顾苏林
Original assignee: Hangzhou Shidai Business Management Co ltd
Current assignee: Hangzhou Shidai Business Management Co ltd
Priority date: 2021-08-16
Filing date: 2021-08-16
Publication date: 2021-11-23

Abstract

A preparation process for extracting antitumor substance sulforaphane from broccoli buds comprises the following steps: harvesting raw materials, sorting raw materials, cleaning, crushing, juicing, concentrating, drying at normal temperature in vacuum, collecting powder, packaging and warehousing. The preparation process can extract, store and maintain the activity of the antitumor substance sulforaphane extracted from broccoli buds to a great extent, and has the core process characteristics that: 1. the crushing rate can reach 90% or more by adopting a five-link double-shaft rolling crushing method and the rolling pressure is 6T. 2. The method comprises the steps of crushing broccoli buds, juicing, stirring at a fast speed to enable a sulforaphane precursor to fully react with black dielectric enzyme, and meanwhile, keeping the temperature not higher than 45 ℃ in a drying process, so that the sulforaphane content in the product is effectively improved, and each 100g of broccoli extract dry powder contains the sulforaphane more than or equal to 1800 u. 3. The method solves the problems of high cost, complex process operation and the like of extracting the sulforaphen from broccoli or dried turnip seeds by adopting an organic solvent or an enzymolysis method, and directly adopts a brand-new crushing technology and a temperature control technology to extract the sulforaphen from broccoli buds.

Description

Preparation process for extracting antitumor substance sulforaphane from broccoli buds

1. Field of the invention

The invention belongs to the technical field of extraction of plant active substances, and particularly relates to a preparation process for extracting antitumor substance sulforaphane from fresh broccoli buds.

2. Background of the invention

Sulforaphane (1-isothiocyanato-4-methanesulfonylbutane) (Sulforaphane), a pharmacological laboratory at Hopkins university, 1992, was extracted from broccoli Sulforaphane. Experiments prove that the sulforaphane plays a role in preventing carcinogens or other metabolites from entering target cells mainly by blocking metabolic pathways required by carcinogens to generate carcinogenic effects, and simultaneously plays a role in inhibiting division and growth of cancer cells, blocking circulation pathways and promoting secretion of other proteins capable of killing the cancer cells. Sulforaphane is a product of dangerous Phase enzyme inducer (carcinogen detoxification) enzymes so far, and further acts on the body's own system to regulate and control metabolism.

Sulforaphane is mainly extracted from dried broccoli seeds or dried radish seeds at present and is obtained by adopting an organic solvent extraction method or an enzymolysis method, and the two extraction processes are complicated to operate and have higher production cost.

The content of sulforaphane in the broccoli buds is more abundant than that in stems and leaves of the broccoli, and the breaking rate of the conventional breaking technology can only reach 40 percent due to the fact that the diameter of the broccoli buds is between 0.05 and 0.1 mm. The production process adopts a five-link double-shaft rolling crushing method, the rolling force is 6T, and the crushing rate can reach 90 percent or more.

The broccoli sulforaphane precursor has no anticancer effect, can generate the sulforaphane only after being matched with the reaction of the black-medium enzyme, but the black-medium enzyme cannot resist high temperature, and loses activity when the temperature is higher than 60 ℃, so that the function of the sulforaphane is easy to disappear. According to the extraction process, the broccoli buds are crushed and juiced, the sulforaphane precursor and the black dielectric enzyme are fully reacted by adopting quick-rotation stirring, meanwhile, the temperature is kept to be not more than 45 ℃ in the drying process, so that the sulforaphane content in the product is effectively improved, and every 100g of broccoli extracted dry powder contains the sulforaphane more than or equal to 1800 u.

3. Summary of the invention

The technical problem to be solved by the invention is as follows: the method solves the problems of high cost, complex process operation and the like of extracting the sulforaphen from broccoli or dried turnip seeds by adopting an organic solvent or an enzymolysis method, and directly adopts a brand-new crushing technology and a temperature control technology to extract the sulforaphen from broccoli buds.

A preparation process for extracting antitumor substance sulforaphane from broccoli buds comprises the following steps:

(9) harvesting raw materials, wherein the broccoli ball is harvested before blooming after the broccoli ball is formed, and both the main ball and the auxiliary ball can be harvested;

(10) sorting raw materials: removing leaves, main stems and impurities, and sorting for 0.5 h;

(11) cleaning and crushing: and after three-stage cleaning, namely rinsing, washing and track spray cleaning, conveying to a crusher, and crushing by adopting five-link double-shaft rolling and crushing with the rolling pressure of 6T. The control time of the steps is 0.5 h;

(12) juicing: conveying the crushed broccoli buds to a belt type juicer for juicing, and separating juice and residues for 20 min;

(13) and (3) storage tank stirring: feeding the broccoli bud juice into a storage tank with a stirring device, and stirring at 20 r/min to allow the sulforaphen to fully react with the black dielectric enzyme for 0.5 h;

(14) concentration: concentrating by double-effect vacuum low-temperature concentration method at evaporation temperature of 40-45 deg.C and water capture rate of 75-80% for 1 hr;

(15) vacuum normal temperature drying: adopting an improved 500L spray drying tower, wherein the height of the spray drying tower is increased by 2.4m compared with the standard spray drying tower, so as to realize temperature control in a drying process period, keeping the temperature in the drying tower at 35-40 ℃, feeding 500L of juice per hour, and taking 50 min;

(16) collecting powder, packaging and warehousing: the water content of the dried broccoli bud extract powder is less than or equal to 6 percent, the broccoli bud extract powder is packaged in 10 kg/barrel, and the storage temperature is controlled at 10-15 ℃.

Claims

1. A preparation process for extracting antitumor substance sulforaphane from broccoli buds is characterized by comprising the following steps:

(1) harvesting raw materials, wherein the broccoli ball is harvested before blooming after the broccoli ball is formed, and both the main ball and the auxiliary ball can be harvested;

(2) sorting raw materials: removing leaves, main stems and impurities, and sorting for 0.5 h;

(3) cleaning and crushing: and after three-stage cleaning, namely rinsing, washing and track spray cleaning, conveying to a crusher, and crushing by adopting five-link double-shaft rolling and crushing with the rolling pressure of 6T. The control time of the steps is 0.5 h;

(4) juicing: conveying the crushed broccoli buds to a belt type juicer for juicing, and separating juice and residues for 20 min;

(5) and (3) storage tank stirring: feeding the broccoli bud juice into a storage tank with a stirring device, and stirring at 20 r/min to allow the sulforaphen to fully react with the black dielectric enzyme for 0.5 h;

(6) concentration: concentrating by double-effect vacuum low-temperature concentration method at evaporation temperature of 40-45 deg.C and water capture rate of 75-80% for 1 hr;

(7) vacuum normal temperature drying: adopting an improved 500L spray drying tower, wherein the height of the spray drying tower is increased by 2.4m compared with the standard spray drying tower, so as to realize temperature control in a drying process period, keeping the temperature in the drying tower at 35-40 ℃, feeding 500L of juice per hour, and taking 50 min;

(8) collecting powder, packaging and warehousing: the water content of the dried broccoli bud extract powder is less than or equal to 6 percent, the broccoli bud extract powder is packaged in 10 kg/barrel, and the storage temperature is controlled at 10-15 ℃.

2. The process according to claim 1, wherein in step (5), the stirring speed is 20 rpm.

3. The process of claim 1, wherein in step (7), the temperature in the drying tower is kept below 45 ℃ all the time, so that the sulforaphane content in the product is effectively increased.