CN105853475A - Preparation method of pemphis acidula extract - Google Patents
Preparation method of pemphis acidula extract Download PDFInfo
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- CN105853475A CN105853475A CN201610001738.9A CN201610001738A CN105853475A CN 105853475 A CN105853475 A CN 105853475A CN 201610001738 A CN201610001738 A CN 201610001738A CN 105853475 A CN105853475 A CN 105853475A
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- flos genkwa
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- acidula
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
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Abstract
The invention discloses a preparation method of a pemphis acidula extract, wherein the preparation method includes the following steps: (1) chopping pemphis acidula, then drying, again crushing and sieving, to obtain a pemphis acidula powder; (2) adding an extracting solvent to the pemphis acidula powder, carrying out ultrasonic extraction, centrifuging the extraction liquid, and then taking a supernatant; (3) carrying out reduced pressure concentration of the supernatant to obtain an extractum; and (4) volatilizing the extractum to make the extracting solvent dried with water bath, and thus obtaining the pemphis acidula extract. The whole preparation method is subjected to optimization design, acetone or absolute ethyl alcohol is used for ultrasonic assisted extraction of pemphis acidula, and then centrifugation and reduced pressure concentration are carried out to obtain the pemphis acidula extract; the preparation method has the advantages of simple process steps, strong operability, high product extraction rate, and good antioxidant capacity and bacteriostatic effect.
Description
Technical field
The present invention relates to effective ingredients in plant extractive technique field, especially relate to a kind of water Flos Genkwa extract preparation method.
Background technology
Water Flos Genkwa (formal name used at school:Pemphis acidula), belonging to undershrub for Lythraceae water Flos Genkwa, distribution is narrow remote, is distributed mainly on China's the island of Taiwan southern coast and other the Eastern Hemisphere tropical coasts, is currently in Critical Condition.
Water Flos Genkwa is often used to make famous and precious potted landscape, research at present is concentrated mainly on its growing environment and distribution, taxonomy, genesiology and genetic diversity etc., there is now partly composition in its plant and the research of pharmacologically active, be primarily directed to water Flos Genkwa extract antioxidation activity in vitro and the research of bacteriostatic activity.But from the point of view of current existing extractive technique, the extraction cost of water Flos Genkwa extract is high, and extraction ratio is low, and product oxidation resistance and fungistatic effect poor, greatly limit hence in so that the popularization of water Flos Genkwa extract receives with research application.
Summary of the invention
The present invention is that the extraction cost of the water Flos Genkwa extract in order to solve prior art is high, extraction ratio is low, and product oxidation resistance and fungistatic effect difference problem, provide a kind of processing step simple, workable, extraction ratio is high, the water Flos Genkwa extract preparation method of product oxidation resistance and good antimicrobial effect.
To achieve these goals, the present invention is by the following technical solutions:
A kind of water Flos Genkwa extract preparation method, comprises the following steps:
(1) water Flos Genkwa is shredded post-drying, then grinding and sieving, obtain water Lilac Daphne Flower Bud.
(2) adding Extraction solvent in water Lilac Daphne Flower Bud, supersound extraction, extracting solution takes supernatant the most afterwards.The present invention is aided with supersound extraction to improve extraction ratio, then under powerful centrifugal action, to increase the dissolution degree of effective active composition in water Lilac Daphne Flower Bud, by ultrasonic and centrifugal coordinated, can be greatly improved the extraction ratio of effective ingredient in water Lilac Daphne Flower Bud.
(3) supernatant obtains extractum after concentrating under reduced pressure.
(4) extractum is after water-bath volatilizes Extraction solvent, obtains water Flos Genkwa extract.
As preferably, in step (1), drying temperature is 30 ~ 50 DEG C.Drying temperature the lowest, efficiency is low, and temperature is the highest, can destroy the effective active composition in water Lilac Daphne Flower Bud, reduces product extraction ratio, oxidation resistance and fungistatic effect.
As preferably, in step (1), cross 60 ~ 100 mesh sieves.Water Lilac Daphne Flower Bud size is the most crucial, meticulous, and production cost is the biggest, the most slightly, is unfavorable for the extraction of effective active composition in water Lilac Daphne Flower Bud, and between 600 ~ 100 mesh, extraction ratio is more or less the same, and therefore the present invention selected 60 ~ 100 mesh sieves.
As preferably, in step (2), water Lilac Daphne Flower Bud is 1:20 ~ 30 with the mass ratio of Extraction solvent.
As preferably, described Extraction solvent is acetone or dehydrated alcohol.Present invention applicant is by strict screening and test, in acetone or dehydrated alcohol, the extraction ratio of water Flos Genkwa extract, oxidation resistance and good antimicrobial effect.
As preferably, in step (2), the technological parameter of supersound extraction is: Extracting temperature 40 ~ 60 DEG C, ultrasonic power 80 ~ 100 W, and ultrasonic time 30 ~ 50 min repeats to extract 3 times.High temperature is conducive to the dissolution of effective active composition in water Lilac Daphne Flower Bud, but temperature is too high, can cause the decomposition of effective active composition in water Lilac Daphne Flower Bud, and therefore ultrasonic temperature controls to be advisable at 40 ~ 60 DEG C；The cavitation energy moment of ultrasound wave causes cell wall to crush the dissolution of effective active composition, diffusion in acceleration water Lilac Daphne Flower Bud, but the impact of the ultrasound intensity of ultrasound wave and cavitation has certain scope, and power 80 ~ 100W extraction rate reached is to maximum magnitude.
As preferably, during supersound extraction, blasting nitrogen every 2 ~ 3min in Extraction solvent, the nitrogen amount of blasting is 0.3 ~ 0.5 m3/h.Ultrasound wave has heat effect simultaneously, the temperature of whole system can be changed, affect extraction ratio, ultrasound wave can form again standing wave in Extraction solvent, making water Lilac Daphne Flower Bud be gathered at node by the effect of mechanical force, for solving this problem, the present invention blasts nitrogen when carrying out supersound extraction every 2 ~ 3min, one is the effect that can play stirring, is conducive to improving the extraction ratio of effective active composition in water Lilac Daphne Flower Bud；Two is the effect playing heat radiation, and heat produced by ultrasound wave can be made to shed in time, is conducive to keeping stablizing of Extracting temperature；Three be passed through nitrogen after, whole material can roll from bottom to top, can destroy the ultrasound wave horizontal standing wave formed in the Extraction solvent, it is to avoid water Lilac Daphne Flower Bud is assembled at node.
Therefore, there is advantages that
(1) whole preparation method is optimized design, uses acetone or dehydrated alcohol that water Flos Genkwa carries out ultrasound assisted extraction, more centrifugal, through being concentrated under reduced pressure to give water Flos Genkwa extract, processing step is simple, workable, product extraction ratio is high, oxidation resistance and good antimicrobial effect；
(2) during supersound extraction, blasting nitrogen every 2 ~ 3min in Extraction solvent, the nitrogen amount of blasting is 0.3 ~ 0.5 m3/ h so that Extracting temperature is more stable, avoids water Lilac Daphne Flower Bud to assemble simultaneously, improves the extraction ratio of effective active composition in water Lilac Daphne Flower Bud.
Detailed description of the invention
Below by detailed description of the invention, the present invention will be further described.
In the present invention, if not refering in particular to, all devices and raw material are all commercially available or the industry is conventional, and wherein, water Flos Genkwa picks up from Xisha, Hainan Province Yongxing Island, the method in following embodiment, if no special instructions, is this area conventional method.
Embodiment 1
(1) crossing 60 mesh sieves after water Flos Genkwa being shredded post-drying, then pulverizing, obtain water Lilac Daphne Flower Bud, drying temperature is 30 DEG C.
(2) during adding Extraction solvent, supersound extraction, supersound extraction in water Lilac Daphne Flower Bud, blasting nitrogen every 2min in Extraction solvent, the nitrogen amount of blasting is 0.3m3/ h, the technological parameter of supersound extraction is: Extracting temperature 40 DEG C, ultrasonic power 80 ~ W, ultrasonic time 30min, repeats to extract 3 times, and united extraction liquid takes supernatant the most afterwards, water Lilac Daphne Flower Bud is 1:20 with the mass ratio of Extraction solvent, and Extraction solvent is acetone or dehydrated alcohol.
(3) supernatant obtains extractum after concentrating under reduced pressure.
(4) extractum is after water-bath volatilizes Extraction solvent, obtains water Flos Genkwa extract.
Embodiment 2
(1) crossing 100 mesh sieves after water Flos Genkwa being shredded post-drying, then pulverizing, obtain water Lilac Daphne Flower Bud, drying temperature is 50 DEG C.
(2) during adding Extraction solvent, supersound extraction, supersound extraction in water Lilac Daphne Flower Bud, blasting nitrogen every 3min in Extraction solvent, the nitrogen amount of blasting is 0.5 m3/ h, the technological parameter of supersound extraction is: Extracting temperature 60 DEG C, ultrasonic power 100 W, ultrasonic time 50 min, repeats to extract 3 times, and united extraction liquid takes supernatant the most afterwards, water Lilac Daphne Flower Bud is 1:30 with the mass ratio of Extraction solvent, and Extraction solvent is acetone or dehydrated alcohol.
(3) supernatant obtains extractum after concentrating under reduced pressure.
(4) extractum is after water-bath volatilizes Extraction solvent, obtains water Flos Genkwa extract.
Embodiment 3
(1) crossing 80 mesh sieves after water Flos Genkwa being shredded post-drying, then pulverizing, obtain water Lilac Daphne Flower Bud, drying temperature is 40 DEG C.
(2) during adding Extraction solvent, supersound extraction, supersound extraction in water Lilac Daphne Flower Bud, blasting nitrogen every 2.5min in Extraction solvent, the nitrogen amount of blasting is 0.4 m3/ h, the technological parameter of supersound extraction is: Extracting temperature 50 DEG C, ultrasonic power 90 W, ultrasonic time 40 min, repeats to extract 3 times, and united extraction liquid takes supernatant the most afterwards, water Lilac Daphne Flower Bud is 1:25 with the mass ratio of Extraction solvent, and Extraction solvent is acetone or dehydrated alcohol.
(3) supernatant obtains extractum after concentrating under reduced pressure.
(4) extractum is after water-bath volatilizes Extraction solvent, obtains water Flos Genkwa extract.
By the method for the present invention, the extraction rate reached more than 11.26% of water Flos Genkwa extract, hydroxyl radical free radical (-OH) clearance rate is reached more than 69.23%, at concentration 6.250 mg ml-1 -3.125 mg·ml-1Between, just escherichia coli, staphylococcus aureus, Haemolysis Vibrio are respectively provided with preferable inhibition.
Embodiment described above is the one preferably scheme of the present invention, and the present invention not makees any pro forma restriction, also has other variant and remodeling on the premise of without departing from the technical scheme described in claim.
Claims (7)
1. a water Flos Genkwa extract preparation method, it is characterised in that comprise the following steps:
(1) water Flos Genkwa is shredded post-drying, then grinding and sieving, obtain water Lilac Daphne Flower Bud；
(2) adding Extraction solvent in water Lilac Daphne Flower Bud, supersound extraction, extracting solution takes supernatant the most afterwards；
(3) supernatant obtains extractum after concentrating under reduced pressure；
(4) extractum is after water-bath volatilizes Extraction solvent, obtains water Flos Genkwa extract.
A kind of water Flos Genkwa extract preparation method the most according to claim 1, it is characterised in that in step (1), drying temperature is 30 ~ 50 DEG C.
A kind of water Flos Genkwa extract preparation method the most according to claim 2, it is characterised in that in step (1), cross 60 ~ 100 mesh sieves.
A kind of water Flos Genkwa extract preparation method the most according to claim 1, it is characterised in that in step (2), water Lilac Daphne Flower Bud is 1:20 ~ 30 with the mass ratio of Extraction solvent.
5. according to a kind of water Flos Genkwa extract preparation method described in claim 1 or 4, it is characterised in that described Extraction solvent is acetone or dehydrated alcohol.
A kind of water Flos Genkwa extract preparation method the most according to claim 1, it is characterised in that in step (2), the technological parameter of supersound extraction is: Extracting temperature 40 ~ 60 DEG C, ultrasonic power 80 ~ 100 W, and ultrasonic time 30 ~ 50 min repeats to extract 3 times.
A kind of water Flos Genkwa extract preparation method the most according to claim 1, it is characterised in that in step (2), during supersound extraction, blasting nitrogen every 2 ~ 3min in Extraction solvent, the nitrogen amount of blasting is 0.3 ~ 0.5 m3/h。
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| CN201610001738.9A CN105853475A (en) | 2016-01-05 | 2016-01-05 | Preparation method of pemphis acidula extract |
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| CN201610001738.9A CN105853475A (en) | 2016-01-05 | 2016-01-05 | Preparation method of pemphis acidula extract |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107752006A (en) * | 2017-10-26 | 2018-03-06 | 哈尔滨工业大学 | A kind of special brain tonic red bean jelly of spacefarer |
| CN107970263A (en) * | 2017-11-17 | 2018-05-01 | 浙江海洋大学 | A kind of extracting method of water lilac daphne flavones |
| CN109134689A (en) * | 2018-11-02 | 2019-01-04 | 浙江海洋大学 | A kind of extracting method of water lilac daphne polysaccharide |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002035760A (en) * | 2000-07-25 | 2002-02-05 | Yoji Hirai | Method for magnetically modifying moisture |
| CN1824084A (en) * | 2005-12-29 | 2006-08-30 | 谢君 | Active component extraction method of plant polyphenol kind substance |
| CN102796067A (en) * | 2012-09-04 | 2012-11-28 | 山东理工大学 | Method for extracting flavonoids from xanthoceras sorbifolia shells |
| CN103304028A (en) * | 2013-06-26 | 2013-09-18 | 哈尔滨工业大学 | Nitrogen stirring method for anaerobic treatment |
-
2016
- 2016-01-05 CN CN201610001738.9A patent/CN105853475A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002035760A (en) * | 2000-07-25 | 2002-02-05 | Yoji Hirai | Method for magnetically modifying moisture |
| CN1824084A (en) * | 2005-12-29 | 2006-08-30 | 谢君 | Active component extraction method of plant polyphenol kind substance |
| CN102796067A (en) * | 2012-09-04 | 2012-11-28 | 山东理工大学 | Method for extracting flavonoids from xanthoceras sorbifolia shells |
| CN103304028A (en) * | 2013-06-26 | 2013-09-18 | 哈尔滨工业大学 | Nitrogen stirring method for anaerobic treatment |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107752006A (en) * | 2017-10-26 | 2018-03-06 | 哈尔滨工业大学 | A kind of special brain tonic red bean jelly of spacefarer |
| CN107970263A (en) * | 2017-11-17 | 2018-05-01 | 浙江海洋大学 | A kind of extracting method of water lilac daphne flavones |
| CN107970263B (en) * | 2017-11-17 | 2021-04-20 | 浙江海洋大学 | Method for extracting flavones of daphne genkwa |
| CN109134689A (en) * | 2018-11-02 | 2019-01-04 | 浙江海洋大学 | A kind of extracting method of water lilac daphne polysaccharide |
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