CN109134689A - A kind of extracting method of water lilac daphne polysaccharide - Google Patents
A kind of extracting method of water lilac daphne polysaccharide Download PDFInfo
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- CN109134689A CN109134689A CN201811301167.6A CN201811301167A CN109134689A CN 109134689 A CN109134689 A CN 109134689A CN 201811301167 A CN201811301167 A CN 201811301167A CN 109134689 A CN109134689 A CN 109134689A
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- lilac daphne
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
Abstract
The present invention relates to technical field of polysaccharide extraction, solve the problems, such as to disclose a kind of extracting method of water lilac daphne polysaccharide containing pigment and impurity particle in polyoses extract.Water lilac daphne strut is first ground into powder by the extracting method, then it carries out being immersed in the water progress cellulase hydrolysis and sonic oscillation extracts to obtain water lilac daphne polysaccharide concentrate, Adsorption impurity particle and pigment in atlapulgite is added in water lilac daphne polysaccharide concentrate, finally carry out de- albumen, alcohol precipitation is dried to obtain water lilac daphne polysaccharide.Water lilac daphne polysaccharide of the present invention eliminates pigment and impurity particle during the preparation process, to greatly improve the purity of water lilac daphne polysaccharide.
Description
Technical field
The present invention relates to technical field of polysaccharide extraction, more particularly, to a kind of extracting method of water lilac daphne polysaccharide.
Background technique
Lilac daphne Pemphis acidula is the limb that Lythraceae water lilac daphne belongs to Pemphis undershrub, belongs to mangrove
One kind, it is less in distribution on global, be distributed mainly on China's the island of Taiwan southern coast and other the Eastern Hemisphere tropical coasts.Due to normal
It is used to the expensive ornamental potted landscape of production, and is denuded, along with the protective awareness of people is weak, present water lilac daphne is
Through endangered state.The distribution and growing environment, classification position, life of water lilac daphne are concentrated mainly on to its existing research at present
, genetic diversity etc. are grown, it is less for its chemical component and Study on Physiological Activity.Mangrove has polysaccharide more abundant
The compounds such as class, flavonoids, terpene, anthocyanin class.At present natural polysaccharide hypoglycemic, immunological regulation, in terms of send out
Biological action is waved, the multiple fields such as medicine, pharmacy and food are widely used in.Plant polyose mentions in the prior art
The method taken usually under the high temperature conditions extracts polysaccharide, since the thermal stability of polysaccharide is poor, polysaccharide at high temperature
Molecular structure changed by broken, to make the active polysaccharide extracted decline, China Patent Publication No. CN107501432 discloses one
Kind gold thread grass polysaccharide extract and the preparation method and application thereof, contains more color in the gold thread grass polysaccharide which obtains
Element and impurity particle, influence the purity of polyoses extract.
Summary of the invention
The present invention is to overcome the problems, such as to provide one kind containing pigment and impurity particle in prior art polyoses extract
The extracting method of water lilac daphne polysaccharide is kept, water lilac daphne polysaccharide of the present invention eliminates pigment and impurity particle during the preparation process, from
And greatly improve the purity of water lilac daphne polysaccharide.
To achieve the goals above, the invention adopts the following technical scheme:
A kind of extracting method of water lilac daphne polysaccharide, comprising the following steps:
1) the water lilac daphne limb after cleaning up is dried, and smashes it through 100-200 mesh and obtains water Lilac Daphne Flower Bud, is placed in
It is saved at aeration-drying, it is spare；
2) water Lilac Daphne Flower Bud is added in distilled water by solid-liquid ratio 1:20-30g/ml, is warming up to 50-55 DEG C, cellulase is added
Enzymatic hydrolysis 30-60min is carried out, then ultrasound extraction 1.5-3h, removal precipitating obtains water lilac daphne polysaccharide filtrate after centrifugation, by water lilac daphne
Polysaccharide filtrate decompression is concentrated into the 10-20% of original volume, obtains water lilac daphne polysaccharide concentrate；
3) modified active carclazyte is added into water lilac daphne polysaccharide concentrate, is placed in shaking table shaking 3-6h, takes supernatant；
4) supernatant is carried out de- albumen 3-5 times using TCA method, stands 1-2h, dehydrated alcohol is added, stands 5- at 3-5 DEG C
12h, centrifugation, takes precipitating, and precipitating is carried out vacuum freeze drying and obtains water lilac daphne polysaccharide.
The present invention can destroy the cell wall of plant by cellulase degradation effect and sonic oscillation, to make in cell
Active constituent dissolve out diffusion, greatly improve the recovery rate of water lilac daphne polysaccharide；Using in modified active bleaching earth adsorption extracting solution
Pigment and little particle impurity, to greatly improve the purity of water lilac daphne polyoses extract.
Preferably, the additive amount of the step 2) cellulase is the 3-8wt% of water Lilac Daphne Flower Bud.
Preferably, ultrasonic power is 150-200W in the step 2).
Preferably, the additive amount of modified active carclazyte is the 10- of water lilac daphne polysaccharide concentrate in the step 3)
20wt%.
Preferably, supernatant and dehydrated alcohol mass ratio 1:4-5 in the step 4).
Preferably, in the step 3) modified active carclazyte preparation method the following steps are included:
It is to impregnate 10-20h in 5-10wt% sulfuric acid solution that concentration, which is added, in montmorillonite, isolates montmorillonite, is dried to obtain activity
Atlapulgite is added in the calcium hydroxide solution that mass concentration is 3-5wt%, is warming up to 60-75 DEG C, is stirred to react 3- by carclazyte
6h is dried to obtain presoma after filtering；By presoma be added mass concentration be 1-5wt% carboxymethyl cellulose aqueous solution in, with
100-200w power sonic oscillation 4-8h, vacuum drying obtains modified active carclazyte after filtering.
The present invention removes small in water lilac daphne polysaccharide concentrate using modified active carclazyte by the method for physical absorption
Grain impurity and pigment.Directly pigment is adsorbed using carclazyte, adsorption efficiency is low, and the present invention is modified place to carclazyte
Reason is added in sulfuric acid solution in montmorillonite, the hydrogen ion that is ionized in acid solution replace between montmorillonite layer tradable calcium, magnesium etc. from
Son improves adsorption effect to expand the distance between montmorillonite layer and layer；Calcium hydroxide can be diffused into montmorillonite inside
Feeling sad property activated centre surface, acid activated centre has suction-operated to calcium hydroxide, to calcium hydroxide be fixed, carboxylic first
Carboxyl when base cellulose is diffused into montmorillonite surface in carboxymethyl cellulose can be formed with the calcium ion in calcium hydroxide matches
Position, so that carboxymethyl cellulose is fixed on montmorillonite, carboxymethyl cellulose and pigment molecular are made due to intermolecular gravitation
With enabling pigment molecular quickly to be adsorbed by montmorillonite, greatly improve the adsorption efficiency of montmorillonite.
Preferably, the mass ratio of the montmorillonite and sulfuric acid solution is 1:25-30.
Preferably, the mass ratio of the atlapulgite and calcium hydroxide solution is 1:5-10.
Preferably, the mass ratio of the presoma and carboxymethyl cellulose aqueous solution is 1:10-15.
Therefore, the invention has the following beneficial effects: the methods for 1) using cellulase cooperation ultrasonic wave added to improve water Yuan
The recovery rate of middle polysaccharide is spent, raw material availability is improved；2) using modified active carclazyte to the particle in water lilac daphne polysaccharide extraction liquid
Impurity and pigment carry out abundant efficient absorption, greatly improve the purity of water lilac daphne polysaccharide product.
Specific embodiment
Below by specific embodiment, technical scheme is described further.
In the present invention, if not refering in particular to, used raw material and equipment etc. are commercially available or commonly used in the art,
Method in embodiment is unless otherwise instructed the conventional method of this field.
Embodiment 1
The preparation method of modified active carclazyte the following steps are included:
It is that 15h is impregnated in 8wt% sulfuric acid solution that concentration, which is added, in montmorillonite, and the mass ratio of montmorillonite and sulfuric acid solution is 1:28,
Montmorillonite is isolated, atlapulgite is dried to obtain, atlapulgite is added in the calcium hydroxide solution that mass concentration is 4wt%,
The mass ratio of atlapulgite and calcium hydroxide solution is 1:8, is warming up to 70 DEG C, 5.5h is stirred to react, before being dried to obtain after filtering
Drive body；It is in 3wt% carboxymethyl cellulose aqueous solution that mass concentration, which is added, in presoma, and presoma is water-soluble with carboxymethyl cellulose
The mass ratio of liquid is 1:13, and with 150w power sonic oscillation 6h, vacuum drying obtains modified active carclazyte after filtering.
A kind of extracting method of water lilac daphne polysaccharide, comprising the following steps:
1) the water lilac daphne limb after cleaning up is dried, and smashes it through 150 meshes and obtains water Lilac Daphne Flower Bud, is placed in ventilation
It is saved at dry, it is spare；
2) water Lilac Daphne Flower Bud is added in distilled water by solid-liquid ratio 1:25g/ml, is warming up to 53 DEG C, cellulase is added and carries out enzyme
50min is solved, the additive amount of cellulase is the 6wt% of water Lilac Daphne Flower Bud, then ultrasound extraction 2h, ultrasonic power 180W, from
Removal precipitating obtains water lilac daphne polysaccharide filtrate after the heart, and water lilac daphne polysaccharide filtrate decompression is concentrated into the 15% of original volume, obtains water
Lilac daphne polysaccharide concentrate；
3) modified active carclazyte is added into water lilac daphne polysaccharide concentrate, the additive amount of modified active carclazyte is that water lilac daphne polysaccharide is dense
The 15wt% of contracting liquid is placed in shaking table shaking 4.5h, takes supernatant；
4) supernatant is carried out de- albumen 4 times using TCA method, stands 1.5h, dehydrated alcohol, supernatant and dehydrated alcohol matter is added
Amount stands 8h than 1:4.5 at 4 DEG C, is centrifuged, takes precipitating, and precipitating is carried out vacuum freeze drying in the case where vacuum degree is 450pa and is obtained
To water lilac daphne polysaccharide.
Embodiment 2
The preparation method of modified active carclazyte the following steps are included:
It is that 12h is impregnated in 6wt% sulfuric acid solution that concentration, which is added, in montmorillonite, and the mass ratio of montmorillonite and sulfuric acid solution is 1:26,
Montmorillonite is isolated, atlapulgite is dried to obtain, the calcium hydroxide solution that mass concentration is 3.5wt% is added in atlapulgite
In, the mass ratio of atlapulgite and calcium hydroxide solution is 1:6, is warming up to 62 DEG C, 5h is stirred to react, before being dried to obtain after filtering
Drive body；It is in 3wt% carboxymethyl cellulose aqueous solution that mass concentration, which is added, in presoma, and presoma is water-soluble with carboxymethyl cellulose
The mass ratio of liquid is 1:13, and with 100w power sonic oscillation 5h, vacuum drying obtains modified active carclazyte after filtering.
A kind of extracting method of water lilac daphne polysaccharide, comprising the following steps:
1) the water lilac daphne limb after cleaning up is dried, and smashes it through 100 meshes and obtains water Lilac Daphne Flower Bud, is placed in ventilation
It is saved at dry, it is spare；
2) water Lilac Daphne Flower Bud is added in distilled water by solid-liquid ratio 1:22g/ml, is warming up to 51 DEG C, cellulase is added and carries out enzyme
35min is solved, the additive amount of cellulase is the 4wt% of water Lilac Daphne Flower Bud, then ultrasound extraction 1.5h, ultrasonic power 150W,
Removal precipitating obtains water lilac daphne polysaccharide filtrate after centrifugation, and water lilac daphne polysaccharide filtrate decompression is concentrated into the 11% of original volume, is obtained
Water lilac daphne polysaccharide concentrate；
3) modified active carclazyte is added into water lilac daphne polysaccharide concentrate, the additive amount of modified active carclazyte is that water lilac daphne polysaccharide is dense
The 12wt% of contracting liquid is placed in shaking table shaking 3.5h, takes supernatant；
4) supernatant is carried out de- albumen 3 times using TCA method, stands 1h, dehydrated alcohol, supernatant and dehydrated alcohol quality is added
Than 1:4.5,6h is stood at 3 DEG C, is centrifuged, takes precipitating, and precipitating is subjected to vacuum freeze drying in the case where vacuum degree is 460pa and is obtained
Water lilac daphne polysaccharide.
Embodiment 3
The preparation method of modified active carclazyte the following steps are included:
It is that 18h is impregnated in 9wt% sulfuric acid solution that concentration, which is added, in montmorillonite, and the mass ratio of montmorillonite and sulfuric acid solution is 1:28,
Montmorillonite is isolated, atlapulgite is dried to obtain, the calcium hydroxide solution that mass concentration is 4.5wt% is added in atlapulgite
In, the mass ratio of atlapulgite and calcium hydroxide solution is 1:8, is warming up to 72 DEG C, is stirred to react 5.5h, is dried to obtain after filtering
Presoma；It is presoma and carboxymethyl cellulose water in 4wt% carboxymethyl cellulose aqueous solution that mass concentration, which is added, in presoma
The mass ratio of solution is 1:14, and with 180w power sonic oscillation 7h, vacuum drying obtains modified active carclazyte after filtering.
A kind of extracting method of water lilac daphne polysaccharide, comprising the following steps:
1) the water lilac daphne limb after cleaning up is dried, and smashes it through 180 meshes and obtains water Lilac Daphne Flower Bud, is placed in ventilation
It is saved at dry, it is spare；
2) water Lilac Daphne Flower Bud is added in distilled water by solid-liquid ratio 1:28g/ml, is warming up to 54 DEG C, cellulase is added and carries out enzyme
55min is solved, the additive amount of cellulase is the 7wt% of water Lilac Daphne Flower Bud, then ultrasound extraction 2.5h, ultrasonic power 180W,
Removal precipitating obtains water lilac daphne polysaccharide filtrate after centrifugation, and water lilac daphne polysaccharide filtrate decompression is concentrated into the 20% of original volume, is obtained
Water lilac daphne polysaccharide concentrate；
3) modified active carclazyte is added into water lilac daphne polysaccharide concentrate, the additive amount of modified active carclazyte is that water lilac daphne polysaccharide is dense
The 18wt% of contracting liquid is placed in shaking table shaking 5.5h, takes supernatant；
4) supernatant is carried out de- albumen 5 times using TCA method, stands 1.5h, dehydrated alcohol, supernatant and dehydrated alcohol matter is added
Amount stands 11h than 1:5 at 5 DEG C, is centrifuged, takes precipitating, and precipitating is carried out vacuum freeze drying in the case where vacuum degree is 470pa and is obtained
To water lilac daphne polysaccharide.
Embodiment 4
The preparation method of modified active carclazyte the following steps are included:
It is that 20h is impregnated in 10wt% sulfuric acid solution that concentration, which is added, in montmorillonite, and the mass ratio of montmorillonite and sulfuric acid solution is 1:30,
Montmorillonite is isolated, atlapulgite is dried to obtain, atlapulgite is added in the calcium hydroxide solution that mass concentration is 5wt%,
The mass ratio of atlapulgite and calcium hydroxide solution is 1:10, is warming up to 75 DEG C, is stirred to react 6h, is dried to obtain forerunner after filtering
Body；It is presoma and carboxymethyl cellulose aqueous solution in 5wt% carboxymethyl cellulose aqueous solution that mass concentration, which is added, in presoma
Mass ratio be 1:15, with 200w power sonic oscillation 8h, vacuum drying obtains modified active carclazyte after filtering.
A kind of extracting method of water lilac daphne polysaccharide, comprising the following steps:
1) the water lilac daphne limb after cleaning up is dried, and smashes it through 200 meshes and obtains water Lilac Daphne Flower Bud, is placed in ventilation
It is saved at dry, it is spare；
2) water Lilac Daphne Flower Bud is added in distilled water by solid-liquid ratio 1:30g/ml, is warming up to 55 DEG C, cellulase is added and carries out enzyme
60min is solved, the additive amount of cellulase is the 8wt% of water Lilac Daphne Flower Bud, then ultrasound extraction 3h, ultrasonic power 200W, from
Removal precipitating obtains water lilac daphne polysaccharide filtrate after the heart, and water lilac daphne polysaccharide filtrate decompression is concentrated into the 20% of original volume, obtains water
Lilac daphne polysaccharide concentrate；
3) modified active carclazyte is added into water lilac daphne polysaccharide concentrate, the additive amount of modified active carclazyte is that water lilac daphne polysaccharide is dense
The 20wt% of contracting liquid is placed in shaking table shaking 6h, takes supernatant；
4) supernatant is carried out de- albumen 5 times using TCA method, stands 2h, dehydrated alcohol, supernatant and dehydrated alcohol quality is added
Than 1:5,12h is stood at 5 DEG C, is centrifuged, takes precipitating, and precipitating is subjected to vacuum freeze drying in the case where vacuum degree is 480pa and is obtained
Water lilac daphne polysaccharide.
Embodiment 5
The preparation method of modified active carclazyte the following steps are included:
It is that 10h is impregnated in 5wt% sulfuric acid solution that concentration, which is added, in montmorillonite, and the mass ratio of montmorillonite and sulfuric acid solution is 1:25,
Montmorillonite is isolated, atlapulgite is dried to obtain, atlapulgite is added in the calcium hydroxide solution that mass concentration is 3wt%,
The mass ratio of atlapulgite and calcium hydroxide solution is 1:5, is warming up to 60 DEG C, is stirred to react 3h, is dried to obtain forerunner after filtering
Body；It is presoma and carboxymethyl cellulose aqueous solution in 1wt% carboxymethyl cellulose aqueous solution that mass concentration, which is added, in presoma
Mass ratio be 1:10, with 100w power sonic oscillation 4h, vacuum drying obtains modified active carclazyte after filtering.
A kind of extracting method of water lilac daphne polysaccharide, comprising the following steps:
1) the water lilac daphne limb after cleaning up is dried, and smashes it through 100 meshes and obtains water Lilac Daphne Flower Bud, is placed in ventilation
It is saved at dry, it is spare；
2) water Lilac Daphne Flower Bud is added in distilled water by solid-liquid ratio 1:20g/ml, is warming up to 50 DEG C, cellulase is added and carries out enzyme
30min is solved, the additive amount of cellulase is the 3wt% of water Lilac Daphne Flower Bud, then ultrasound extraction 1.5h, ultrasonic power 150W,
Removal precipitating obtains water lilac daphne polysaccharide filtrate after centrifugation, and water lilac daphne polysaccharide filtrate decompression is concentrated into the 10% of original volume, is obtained
Water lilac daphne polysaccharide concentrate；
3) modified active carclazyte is added into water lilac daphne polysaccharide concentrate, the additive amount of modified active carclazyte is that water lilac daphne polysaccharide is dense
The 10wt% of contracting liquid is placed in shaking table shaking 3h, takes supernatant；
4) supernatant is carried out de- albumen 3 times using TCA method, stands 1h, dehydrated alcohol, supernatant and dehydrated alcohol quality is added
Than 1:4,5h is stood at 3 DEG C, is centrifuged, takes precipitating, and precipitating is subjected to vacuum freeze drying in the case where vacuum degree is 490pa and obtains water
Lilac daphne polysaccharide.
The measurement of water lilac daphne polysaccharide:
This test measures the content of water lilac daphne polysaccharide using Phenol sulfuric acid procedure, with glucose as a standard product.Precision weighs 105 DEG C and does
The dry glucose 0.0010g to constant weight, is placed in 100mL volumetric flask, adds distilled water to dissolve and is diluted to scale, shakes up, with
Glucose standards solution.Precision draws glucose standards solution 0,0.2,0.4,0.6,0.8,1.0mL, is respectively placed in 10mL capacity
In bottle, distilled water is added and mends to 2mL, then adds 5% phenol solution 1mL, shakes up, be rapidly added concentrated sulfuric acid 5mL.It shakes up,
After boiling water bath 15min, it is cooled to room temperature in cold water.Absorbance is surveyed at 490nm wavelength.Finally respectively with each dense of glucose
Degree is abscissa, using absorbance value as ordinate, draws standard curve.Equation of linear regression: y=7.1x+0.1058, R=
0.9969。
The calculating of water lilac daphne polysaccharide extract rate
The water lilac daphne polysaccharide and constant volume extracted with secondary distilled water dissolution according to the above method, measures extinction in 100mL volumetric flask
It spends (in parallel three times), PAP content is calculated by glucose standard curve, according to the following formula the recovery rate of water lilac daphne polysaccharide:
Water lilac daphne polysaccharide extract rate X (%)=(m/M) × 100%；
Wherein: m is the content of polysaccharide in water lilac daphne Thick many candies；M is the quality of water Lilac Daphne Flower Bud.
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | |
| Recovery rate (%) | 3.24 | 3.12 | 3.35 | 3.08 | 3.47 |
The above described is only a preferred embodiment of the present invention, be not intended to limit the present invention in any form, though
So the present invention has been disclosed as a preferred embodiment, and however, it is not intended to limit the invention, any technology people for being familiar with this profession
Member, without departing from the scope of the present invention, when the technology contents using the disclosure above make a little change or modification
It is right according to the technical essence of the invention for the equivalent embodiment of equivalent variations, but without departing from the technical solutions of the present invention
Any simple modification, equivalent change and modification made by above embodiments, all of which are still within the scope of the technical scheme of the invention.
Claims (9)
1. a kind of extracting method of water lilac daphne polysaccharide, which comprises the following steps:
1) the water lilac daphne limb after cleaning up is dried, and smashes it through 100-200 mesh and obtains water Lilac Daphne Flower Bud, is placed in
It is saved at aeration-drying, it is spare；
2) water Lilac Daphne Flower Bud is added in distilled water by solid-liquid ratio 1:20-30g/ml, is warming up to 50-55 DEG C, cellulase is added
Enzymatic hydrolysis 30-60min is carried out, then ultrasound extraction 1.5-3h, removal precipitating obtains water lilac daphne polysaccharide filtrate after centrifugation, by water lilac daphne
Polysaccharide filtrate decompression is concentrated into the 10-20% of original volume, obtains water lilac daphne polysaccharide concentrate；
3) modified active carclazyte is added into water lilac daphne polysaccharide concentrate, is placed in shaking table shaking 3-6h, takes supernatant；
4) supernatant is carried out de- albumen 3-5 times using TCA method, stands 1-2h, dehydrated alcohol is added, stands 5- at 3-5 DEG C
12h, centrifugation, takes precipitating, and precipitating is carried out vacuum freeze drying and obtains water lilac daphne polysaccharide.
2. a kind of extracting method of water lilac daphne polysaccharide according to claim 1, which is characterized in that fiber in the step 2
The additive amount of plain enzyme is the 3-8wt% of water Lilac Daphne Flower Bud.
3. a kind of extracting method of water lilac daphne polysaccharide according to claim 1, which is characterized in that ultrasonic in the step 2
Power is 150-200W.
4. a kind of extracting method of water lilac daphne polysaccharide according to claim 1, which is characterized in that modified in the step 3)
The additive amount of atlapulgite is the 10-20wt% of water lilac daphne polysaccharide concentrate.
5. a kind of extracting method of water lilac daphne polysaccharide according to claim 1, which is characterized in that supernatant in the step 4)
Liquid and dehydrated alcohol mass ratio 1:4-5.
6. a kind of extracting method of water lilac daphne polysaccharide according to claim 1 or 4, which is characterized in that in the step 3)
The preparation method of modified active carclazyte the following steps are included:
It is to impregnate 10-20h in 5-10wt% sulfuric acid solution that concentration, which is added, in montmorillonite, isolates montmorillonite, it is white to be dried to obtain activity
Atlapulgite is added in the calcium hydroxide solution that mass concentration is 3-5wt%, is warming up to 60-75 DEG C, is stirred to react 3-6h by soil,
Presoma is dried to obtain after filtering；It is in 1-5wt% carboxymethyl cellulose aqueous solution, with 100- that mass concentration, which is added, in presoma
200w power sonic oscillation 4-8h, vacuum drying obtains modified active carclazyte after filtering.
7. a kind of extracting method of water lilac daphne polysaccharide according to claim 6, which is characterized in that the montmorillonite and sulfuric acid
The mass ratio of solution is 1:25-30.
8. a kind of extracting method of water lilac daphne polysaccharide according to claim 6, which is characterized in that the atlapulgite and hydrogen
The mass ratio of calcium oxide solution is 1:5-10.
9. a kind of extracting method of water lilac daphne polysaccharide according to claim 8, which is characterized in that the presoma and carboxylic first
The mass ratio of base cellulose aqueous solution is 1:10-15.
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| CN101658783A (en) * | 2009-09-15 | 2010-03-03 | 广西大学 | Loaded calcium hydroxide activity carclazyte and preparation method thereof |
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| CN106423219A (en) * | 2016-09-22 | 2017-02-22 | 山东瑞捷新材料有限公司 | Method for producing trimellitate using solid acid-loaded active clay as catalyst |
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2018
- 2018-11-02 CN CN201811301167.6A patent/CN109134689A/en active Pending
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101658783A (en) * | 2009-09-15 | 2010-03-03 | 广西大学 | Loaded calcium hydroxide activity carclazyte and preparation method thereof |
| CN105853475A (en) * | 2016-01-05 | 2016-08-17 | 浙江海洋学院 | Preparation method of pemphis acidula extract |
| CN106423219A (en) * | 2016-09-22 | 2017-02-22 | 山东瑞捷新材料有限公司 | Method for producing trimellitate using solid acid-loaded active clay as catalyst |
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Application publication date: 20190104 |