CN113683188A - Method and device for electrochemically domesticating anaerobic ammonium oxidation bacteria - Google Patents
Method and device for electrochemically domesticating anaerobic ammonium oxidation bacteria Download PDFInfo
- Publication number
- CN113683188A CN113683188A CN202111067737.1A CN202111067737A CN113683188A CN 113683188 A CN113683188 A CN 113683188A CN 202111067737 A CN202111067737 A CN 202111067737A CN 113683188 A CN113683188 A CN 113683188A
- Authority
- CN
- China
- Prior art keywords
- carbon fiber
- reactor
- sludge
- nitrogen
- water
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/005—Combined electrochemical biological processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2203/00—Apparatus and plants for the biological treatment of water, waste water or sewage
- C02F2203/004—Apparatus and plants for the biological treatment of water, waste water or sewage comprising a selector reactor for promoting floc-forming or other bacteria
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/06—Controlling or monitoring parameters in water treatment pH
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/14—NH3-N
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/15—N03-N
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2209/00—Controlling or monitoring parameters in water treatment
- C02F2209/22—O2
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Sustainable Development (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Water Supply & Treatment (AREA)
- Electromagnetism (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Electrochemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
- Water Treatment By Electricity Or Magnetism (AREA)
Abstract
The invention discloses a method and a device for electrochemically domesticating anaerobic ammonia oxidizing bacteria, belonging to the technical field of water treatment, wherein the device comprises a reactor main body, a water inlet system connected with a reactor main body pipeline and a sludge reflux device connected with the reactor main body pipeline; the reactor main body comprises a microbial electrochemical reactor, wherein an anode carbon fiber brush, a reference electrode and a cathode carbon fiber brush are arranged in the microbial electrochemical reactor; the invention uses carbon fiber with good conductivity, good biological affinity, economy and environmental protection as a biomembrane carrier, applies the microbial electrochemical principle, strengthens the activity of anaerobic ammonia oxidation bacteria, shortens the starting time of the anaerobic ammonia oxidation process, realizes long-term stable operation, and provides a theoretical basis for the wide application of a novel biological denitrification technology.
Description
Technical Field
The invention relates to the technical field of water treatment, in particular to a method and a device for electrochemically domesticating anaerobic ammonium oxidation bacteria.
Background
The sewage treatment method can be divided into a physical chemical method and a biological method, wherein the biological method is widely applied due to good treatment effect; meanwhile, the traditional biological method needs to add a carbon source, so that secondary pollution is increased. Therefore, the new biological denitrification technology-anaerobic ammonia oxidation technology is a new direction for solving the problem.
The ANAMMOX (ANAMMOX) technology is widely researched as a novel biological denitrification technology, and compared with the traditional nitrification and denitrification technology, the anaerobic ammonium oxidation (ANAMMOX) technology has the advantages of no need of an external carbon source, no need of aeration, low sludge yield and the like. Under anaerobic conditions, anammox bacteria can utilize nitrite (N0)2 --N) and Ammonia Nitrogen (NH)4 +-N) as electron acceptor and electron donor, with a one-step conversion to nitrogen. However, the practical application of the anammox technology has some defects, and the anammox technology is long in starting time due to slow growth of anammox bacteria and long cell doubling time; and the anammox bacteria are extremely sensitive to environmental conditions, such as organic matters, dissolved oxygen, salinity, heavy metal ions and the like in the sewage and wastewater. Therefore, the rapid enrichment of the anammox bacteria is always a first breakthrough problem of the process, and the research on a new enrichment method and a new enrichment device has important significance for promoting the large-scale application of the anammox process.
Disclosure of Invention
The invention aims to provide a method and a device for electrochemically domesticating anammox bacteria, which are used for solving the problems in the prior art, promoting the metabolism of the anammox bacteria, and accelerating the proliferation and rapid enrichment of the anammox bacteria.
In order to achieve the purpose, the invention provides the following scheme:
the invention provides a device for electrochemically domesticating anaerobic ammonia oxidizing bacteria, which comprises a reactor main body, a water inlet system and a sludge reflux device, wherein the water inlet system is connected with a pipeline of the reactor main body; the reactor main body comprises a microbial electrochemical reactor, wherein an anode carbon fiber brush, a reference electrode and a cathode carbon fiber brush are arranged in the microbial electrochemical reactor; the anode carbon fiber brush and the cathode carbon fiber brush are respectively connected with a potentiostat.
Further, the reactor main body also comprises a magnetic stirrer, and a rotor of the magnetic stirrer is an octagonal rotor.
Furthermore, the anode carbon fiber brush and the cathode carbon fiber brush are wound and fixed on the top of the microbial electrochemical reactor through titanium wires and are connected with the potentiostat through the titanium wires.
Further, the reference electrode is an Ag/AgCl electrode.
Further, the water inlet system comprises a nitrogen cylinder, a DO/pH monitor I, a water inlet pump and a water storage tank; an aeration pipe is arranged at the bottom of the water storage tank and is connected with the nitrogen bottle through a pipeline; the upper part of the water storage tank is provided with a water filling port and a one-way air outlet valve; and a heating rod is arranged in the water storage tank.
Furthermore, the sludge backflow system comprises a water outlet peristaltic pump, a backflow sludge peristaltic pump and a secondary sedimentation tank, wherein a water intake at the upper part of the secondary sedimentation tank is arranged on the secondary sedimentation tank.
The invention also provides a method for electrochemically domesticating anammox bacteria, which adopts the device for electrochemically domesticating anammox bacteria, and comprises the following steps:
(1) inoculating the inoculated sludge into a microbial electrochemical reactor;
(2) controlling the water temperature of the nitrogen-containing sewage in the water storage tank to be 35 ℃ by a heating rod; opening a nitrogen bottle, and removing oxygen by blowing through an aeration pipe for 20-30 min;
(3) injecting nitrogen-containing sewage into a microbial electrochemical reactor through a water inlet pump;
(4) starting the magnetic stirrer at the rotation speed of 100-;
(5) monitoring the pH within the microbial electrochemical reactor to maintain the pH between 7.5 and 8.2;
(6) the hydraulic retention time is 23h, wherein the water inlet and the water outlet are respectively kept still for 15 min.
Further, in the step (1), the inoculated sludge is anaerobic activated sludge, anoxic activated sludge and anaerobic ammonia oxidation sludge in a volume ratio of 5:4: 1; the MLSS value of the inoculated sludge is 2000-4000 mg/L.
Further, in the step (2), after the nitrogen-containing sewage is subjected to oxygen removal by blowing, the DO value is less than 0.5mg/L, and the pH is adjusted to be between 7.5 and 8.2 by adding acid and alkali.
Further, in the step (3), the average ammonia nitrogen content of the influent nitrogen-containing sewage is 50mg/L, and the average nitrite nitrogen content is 66 mg/L.
The invention discloses the following technical effects:
the carbon fiber brush used in the invention has good conductivity and the advantage of large biological retention amount, and provides an attachment place for anaerobic ammonium oxidation bacteria. The electrochemical technology is used for domesticating the anaerobic ammonia oxidizing bacteria, has high efficiency and operation flexibility, can change the morphological structure of the anaerobic ammonia oxidizing bacteria, catalyze some proteases in cells of the anaerobic ammonia oxidizing bacteria, accelerate the transmission between electrons and electrodes in the cells of the anaerobic ammonia oxidizing bacteria, promotes the proliferation of the anaerobic ammonia oxidizing bacteria through a three-electrode microorganism electrochemical device, shortens the starting time of an anaerobic ammonia oxidizing process, and finally aims to improve the denitrification capacity of the anaerobic ammonia oxidizing bacteria. In addition, on the premise of not arranging a three-phase separator, the sludge return system avoids the loss of biomass.
The invention uses carbon fiber with good conductivity, good biological affinity, economy and environmental protection as a biomembrane carrier, applies the microbial electrochemical principle, strengthens the activity of anaerobic ammonia oxidation bacteria, shortens the starting time of the anaerobic ammonia oxidation process, realizes long-term stable operation, and provides a theoretical basis for the wide application of a novel biological denitrification technology.
The invention discloses a microbial electrochemical system capable of improving the biological activity of functional bacteria, and the microbial electrochemical technology is used for domesticating anaerobic ammonia oxidizing bacteria, so that a novel method for domesticating anaerobic ammonia oxidizing bacteria in an electrochemical manner is developed, and the method has practical significance for solving the limitation of the existing anaerobic ammonia oxidizing technology.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a schematic structural diagram of an apparatus for acclimating anammox bacteria by an electrochemical method;
wherein, a nitrogen cylinder 1, a heating rod 2, an aeration pipe 3, a DO/pH monitor I4, a water inlet pump 5, a water storage tank 6, a water injection hole 7, a one-way gas outlet valve 8, an anode carbon fiber brush 9, a retention port 10, a cathode carbon fiber brush 11, a reference electrode 12, an octagonal rotor 13, a gas outlet one-way valve 14, a DO/pH monitor II 15, a heating rod 16, a water outlet peristaltic pump 17, a return sludge peristaltic pump 18, a secondary sedimentation tank 19, a secondary sedimentation tank upper water intake 20, a potentiostat 21, a magnetic stirrer 22 and a microbial electrochemical reactor 23;
FIG. 2 shows ammonia Nitrogen (NH) in acclimatization process4 +-N) a variation graph;
FIG. 3 is nitrite Nitrogen (NO) of acclimatization process2 --N) a variation graph;
FIG. 4 is acclimatization process nitrate Nitrogen (NO)3 --N) change maps.
Detailed Description
Reference will now be made in detail to various exemplary embodiments of the invention, the detailed description should not be construed as limiting the invention but as a more detailed description of certain aspects, features and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. Further, for numerical ranges in this disclosure, it is understood that each intervening value, between the upper and lower limit of that range, is also specifically disclosed. Every smaller range between any stated value or intervening value in a stated range and any other stated or intervening value in a stated range is encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference herein for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the present disclosure without departing from the scope or spirit of the disclosure. Other embodiments will be apparent to those skilled in the art from consideration of the specification. The description and examples are intended to be illustrative only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are open-ended terms that mean including, but not limited to.
The embodiment of the invention provides a device for electrochemically domesticating anaerobic ammonia oxidizing bacteria, which comprises a reactor main body, a water inlet system and a sludge reflux device, wherein the water inlet system is connected with a pipeline of the reactor main body; wherein, the reactor main part includes microorganism electrochemical reactor 23, is provided with positive pole carbon fiber brush 9, reference electrode 12 and negative pole carbon fiber brush 11 in the microorganism electrochemical reactor 23.
Wherein, the microbial electrochemical reactor 23 is a closed cylinder made of acrylic plates, the outer part of the microbial electrochemical reactor is shielded by shading cloth, the wall thickness of the container is 2cm, the effective height is 10-15cm, preferably 15cm, the inner diameter is 15-20cm, preferably 15cm, and the effective volume is 1.5L; a round reserving opening 10 with the diameter of 3cm is arranged at the upper part of the microbial electrochemical reactor 23 and is plugged by a rubber plug, so that the change of the interior of the reactor during operation can be observed, the operation condition can be adjusted, and the DO or the pH value of the sewage can be adjusted; meanwhile, a DO/pH monitor II 15 is arranged at the upper part of the microbial electrochemical reactor 23 and is used for detecting the DO value and the pH value of the sewage in the reaction process so as to adjust the condition of the sewage at any time.
As a preferable scheme of the invention, the anode carbon fiber brush 9 and the cathode carbon fiber brush 11 are cylindrical, the diameter is 3cm, the length of the brush hair is 13-15cm, preferably 13cm, the brush hair is wound and fixed on the top of the microbial electrochemical reactor 23 through a titanium wire, and the titanium wire extends out of the microbial electrochemical reactor for 235cm, so that the titanium wire is connected with an alligator clip of the potentiostat 21; the volume of the two carbon fiber brushes is 40% of the microbial electrochemical reactor 23.
As the preferred scheme of the invention, the reference electrode 12 is an Ag/AgCl electrode, the electrode potential is stable, the reference electrode can be used in the microbial electrochemical reactor 23 for a long time, and the reference electrode has the advantages of long service life and good reproducibility; the working electrode and the counter electrode are two carbon fiber brushes.
As a further preferable scheme of the present invention, the potentiostat 21 is a CHI1030C multichannel potentiostat, provides electrode potential for the carbon fiber brush, and can be used for electrochemical tests under the condition of simultaneous operation of multiple reaction devices and multiple working electrodes, thereby improving the overall operation efficiency.
As a further preferred embodiment of the present invention, the carbon fiber content of each of the anode carbon fiber brush 9 and the cathode carbon fiber brush 11 is 10g, the lower end of the carbon fiber is adhered to the bottom of the microbial electrochemical reactor 23 by a glue stick, and the carbon fiber has various characteristics of high temperature resistance, electric conduction, heat conduction, and the like, thereby providing a convenient place for attachment, inhabitation and metabolism of anammox bacteria.
As the preferred scheme of the invention, the reactor main body also comprises a magnetic stirrer 22, the rotor of the magnetic stirrer 22 is an octagonal rotor 13, and the octagonal rotor is adopted, so that on one hand, the pressure intensity to the microbial electrochemical reactor 23 is reduced, and the reactor is protected; on the other hand, the octagonal rotor 13 is more stable than the common rotor in rotation, thereby being beneficial to fully and uniformly mixing anaerobic ammonia-oxygen sludge and sewage, accelerating the nitrogen treatment efficiency and improving the operating efficiency of the reactor.
As the preferred scheme of the invention, the water inlet system comprises a nitrogen cylinder 1, a DO/pH monitor I4, a water inlet pump 5 and a water storage tank 6; the bottom of the water storage tank 6 is provided with an aeration pipe 3, and the aeration pipe 3 is connected with a nitrogen bottle 1 through a pipeline and is used for removing oxygen from the nitrogen-containing sewage; the upper part of the water storage tank 6 is provided with a water filling port 7 and a one-way air outlet valve 8; the heating rod 2 is arranged in the water storage tank 6 and is used for adjusting the water temperature of the nitrogen-containing sewage. The aeration pipe 3 is enclosed into a circle and is arranged at the bottom of the water storage tank 6, which is beneficial to uniform aeration, increases the aeration efficiency and shortens the aeration time.
As a preferred scheme of the invention, the sludge reflux system comprises a water outlet peristaltic pump 17, a reflux sludge peristaltic pump 18 and a secondary sedimentation tank 19, wherein a water intake 20 at the upper part of the secondary sedimentation tank is arranged on the secondary sedimentation tank 19 and is used for collecting water samples to enter and exit and monitoring the growth condition of anaerobic ammonium oxidation bacteria.
The sludge backflow system is connected with the two channels of the microbial electrochemical reactor 23 and is used for backflow of sewage and sludge, partial sludge is prevented from being discharged out of the microbial electrochemical reactor 23 along with discharged water, the sludge concentration in the reactor is effectively guaranteed through the arrangement, and favorable guarantee is provided for long-term stable operation of anaerobic ammonia oxidation.
The embodiment of the invention also provides a method for electrochemically domesticating anammox bacteria, which adopts the device for electrochemically domesticating anammox bacteria and comprises the following steps:
(1) inoculating anaerobic activated sludge, anoxic activated sludge and anaerobic ammonium oxidation sludge of urban domestic sewage plants in Zhenjiang city of Jiangsu province into a microbial electrochemical reactor 23 according to the volume ratio of 5:4: 1;
(2) will contain ammonia Nitrogen (NH)4 +-N) and nitrite Nitrogen (NO)2 --N) simulated nitrogenous sewage is injected into the tank 6, the temperature is maintained at 35 ℃ by the heating rod 2; opening nitrogen cylinder 1, using pureRemoving oxygen from nitrogen with a degree of 99.5% by blowing through an aeration pipe 3 for 20-30min, preferably 25min, so that DO value of the nitrogen-containing sewage in the water storage tank 6 is maintained below 0.5mg/L, and adjusting pH of the nitrogen-containing sewage to 7.5-8.2 by using 1M sodium bicarbonate solution or 1M dilute hydrochloric acid;
(3) injecting nitrogen-containing sewage into a microbial electrochemical reactor 23 through a water inlet pump 5, wherein the total volume of inoculated sludge and the nitrogen-containing sewage is 1.5L, immersing an anode carbon fiber brush 9 and a cathode carbon fiber brush 11, and placing a reference electrode 12 at a position 3cm below the water surface;
(4) starting the magnetic stirrer 22 at a rotation speed of 100-;
(5) monitoring the pH inside the microbial electrochemical reactor 23 and adjusting it by means of a retention port 10 (adjustment with 1M sodium bicarbonate solution or 1M dilute hydrochloric acid) to maintain the pH between 7.5 and 8.2;
(6) setting the hydraulic retention time to be 23h, wherein standing for 30min, including water inlet and water outlet for 15min respectively.
As a further preferable embodiment of the present invention, the MLSS value of the inoculated sludge is 2000-4000mg/L, preferably 3500 mg/L.
As a further preferable scheme of the invention, in the step (3), the average ammonia nitrogen content of the influent nitrogen-containing sewage is 50mg/L, and the average nitrite nitrogen content is 66 mg/L.
Example 1
(1) 4 identical microbial electrochemical reactors were constructed, designated R1, R2, R3, R4, respectively, wherein R1 was not applied with a potential (as a control group), i.e. Open Circuit (OC) mode. Electrode potentials of 0.003V, -0.197V, -0.397V (vs. Ag/AgCl) were applied to R2, R3, R4, respectively (three experimental groups). The CHI1030C multichannel potentiostat from Shanghai Chenghua applies corresponding electrode potentials to the reactors.
(2) And the reactors are respectively inoculated with anoxic tank sludge, anaerobic tank sludge and anaerobic ammonium oxidation sludge of urban sewage plants in Zhenjiang city, Jiangsu province, wherein the inoculation volumes of the anoxic tank sludge, the anaerobic tank sludge and the anaerobic ammonium oxidation sludge are respectively 50ml, 40ml and 10 ml.
(3) The water storage tank is filled with simulated sewage.
The simulated wastewater had the following composition: 2mg/LKH2PO4、110mg/L KHCO3、20mg/L MgSO4·7H2O、20mg/L CaCl2·2H2O, 1.5mL/L microelement I and 1.5mL/L microelement II.
The trace element I is EDTA-2 Na 5g/L and FeSO 5g/L4·7H2O; the microelements II are 15g/L EDTA and 0.25g/L CuSO4·5H2O、0.99g/L MnCl2·4H2O、0.43g/L ZnSO4·7H2O、0.24g/L CoCl2·6H2O、0.19g/L NiCl2·6H2O and 0.22g/L NaMoO4·2H2O。
NH4 +-N is 50mg/L, NO2 -N is 66 mg/L.
Substrate NH4 +-N and NO2 -N is NH4Cl and NaNO2Provided is a method.
(4) And (3) evaluation of denitrification effect: throughout the acclimatization process, NH was monitored daily4 +-N、NO2 --N、NO3 --the concentration profile of N.
Results of the experiment
1) And (3) a thallus hydrolysis period: r2 and R3 are on days 1-3, R1 and R4 are on days 1-6,
average feed water NH4 +-N and mean feedwater NO2 -N is 50mg/L and 66 mg/L. The sludge just inoculated to the microbial electrochemical reactor fails to adapt to new environment, so that bacteria are cracked and autolyzed, and NH in water is discharged4 +The N concentration is higher than the feed water. No NO was detected in the effluent at this stage2 -N, due to the denitrifying bacteria utilizing NO in the influent water2 -N undergoes a strong denitrification reaction. In addition, the anaerobic ammonia oxidation reaction generates nitrate nitrogen, and the activity of anaerobic ammonia oxidation can be calculated by monitoring the content of the nitrate nitrogen in effluent. In the bacterial hydrolysis stage, the nitrate nitrogen content was not detected, and denitrification reaction using nitrate nitrogen as a substrate could occur or anaerobic ammonium oxidation activity was not exhibited.
NO2 -+3H (electron donor-organic) → 0.5N2+H2O+OH-
NO3 -+5H (electron donor-organic) → 0.5N2+2H2O+OH-
2) An activity-increasing period: 7 th to 28 th days of R2, R3 and R4 and 11 th to 28 th days of R1.
As the number of days of acclimatization increases, the effluent NH4 +The N concentration gradually decreased, and since no potential was applied to the R1 reactor, the time to enter the period of increased anammox activity was slower than in the R2, R3, R4 reactors where three different electrode potentials were applied. As can be seen from FIG. 2, NH4 +Increasing N removal, NH4 +-N removal rate order is R1<R2<R3<And R4. Average NH in reactors 20d-27d, R1, R2, R3, R44 +the-N removal rates were 71.43%, 93.36%, 94.76% and 96.73%, respectively. It can be seen that three reactors NH are applied with a potential4 +The N removal rate is higher than in a reactor without applied potential. Wherein NH is performed at an optimum potential of-0.397V4 +the-N removal rate is as high as 96.73%. The removal rate is 25.3% higher than that of the R1 reactor without applied potential.
As shown in FIG. 3, NO in the R1, R2, R3, R4 reactors2 -The N removal rate gradually decreases from 98%, 94%, 99%, 92% at the beginning of the present phase to 17% at 22d, 16% at 19d, 28% at 19d, 39% at 19 d. No of R1 22 nd, R2, R3, R4 19 th d in this experiment2 -the-N removal rate is the lowest. R1 after 19d, R2, R3, R4 after 22d, NO2 -the-N removal rate is inversely increased. NO2 -The reason for the sudden drop in the removal rate of-N is that the reduction of denitrifying bacteria and the disappearance of autolysis promote NO2 --N presents a residual accumulation; the reason why the removal rate rises again later is that the abundance and activity of anammox bacteria increase with the increase of acclimation time, and the rising rate sequence is R1<R2<R3<R4, indicating that NO can be accelerated by an applied electrode potential2 --N removal. Promoting substrate NO2 -Anaerobic ammoniaThe oxidizing bacteria are consumed as electron acceptors and the turning point also represents an increase in the activity of the anammox bacteria. It has been shown that NO2 -The gradual decrease in-N removal from 100% indicates a decrease in denitrification, so NO2 -The removal rate of-N is increased, then decreased and then increased from low, which indicates that the activity of the anaerobic ammonia oxidation reaction is enhanced.
As shown in FIG. 4, NO in R1, R2, R3 and R43 -The production of N is gradually increased and the order of the rising speeds is R4>R3>R2>And R1. According to the anammox reaction formula, it was concluded that anammox bacteria had the highest activity when an electrode potential of-0.397V was applied. No. 24d-28d, R1, R2, R3 and R4 average effluent3 -The N concentrations were 6mg/L, 9mg/L, 10mg/L and 15mg/L, respectively, and it can be seen that the effluent concentration of R4 nitrate nitrogen was the highest. It was again confirmed that the anammox bacteria were most active when a potential of-0.397V was applied.
Therefore, the potential is applied to obviously promote the anammox denitrification capability. This shows that the microbial electrochemical system has a promoting effect on biological metabolism and accelerates the proliferation of anammox bacteria, thus demonstrating the feasibility of the technical scheme of the invention.
The above-described embodiments are merely illustrative of the preferred embodiments of the present invention, and do not limit the scope of the present invention, and various modifications and improvements of the technical solutions of the present invention can be made by those skilled in the art without departing from the spirit of the present invention, and the technical solutions of the present invention are within the scope of the present invention defined by the claims.
Claims (10)
1. The device for electrochemically domesticating the anaerobic ammonia oxidizing bacteria is characterized by comprising a reactor main body, a water inlet system connected with a pipeline of the reactor main body and a sludge reflux device connected with a pipeline of the reactor main body; the reactor main body comprises a microbial electrochemical reactor (23), wherein an anode carbon fiber brush (9), a reference electrode (12) and a cathode carbon fiber brush (11) are arranged in the microbial electrochemical reactor (23); the anode carbon fiber brush (9) and the cathode carbon fiber brush (11) are respectively connected with a potentiostat (21).
2. The apparatus according to claim 1, wherein the reactor body further comprises a magnetic stirrer (22), the rotor of the magnetic stirrer (22) being an octagonal rotor (13).
3. The device according to claim 1, characterized in that the anodic carbon fiber brush (9) and the cathodic carbon fiber brush (11) are fixed on top of the microbial electrochemical reactor (23) by means of titanium wire winding and connected with the potentiostat (21) by means of titanium wires.
4. The device according to claim 1, characterized in that the reference electrode (12) is an Ag/AgCl electrode.
5. The apparatus according to claim 1, wherein the water inlet system comprises a nitrogen gas cylinder (1), a DO/pH monitor I (4), a water inlet pump (5) and a water storage tank (6); an aeration pipe (3) is arranged at the bottom of the water storage tank (6), and the aeration pipe (3) is connected with the nitrogen bottle (1) through a pipeline; the upper part of the water storage tank (6) is provided with a water filling port (7) and a one-way air outlet valve (8); and a heating rod (2) is arranged in the water storage tank (6).
6. The device according to claim 1, characterized in that the sludge return system comprises a water outlet peristaltic pump (17), a return sludge peristaltic pump (18) and a secondary sedimentation tank (19), and a water intake (20) at the upper part of the secondary sedimentation tank is arranged on the secondary sedimentation tank (19).
7. A method for electrochemically acclimating anammox bacteria, using the device for electrochemically acclimating anammox bacteria of any one of claims 1-6, comprising the steps of:
1) inoculating the inoculated sludge into a microbial electrochemical reactor (23);
2) controlling the water temperature of the nitrogen-containing sewage in the water storage tank (6) to be 35 ℃ through the heating rod (2); opening a nitrogen bottle (1), and removing oxygen by blowing through an aeration pipe (3) for 20-30 min;
3) injecting nitrogen-containing sewage into a microbial electrochemical reactor (23) through a water inlet pump (5);
4) starting the magnetic stirrer (22) at the rotation speed of 100-;
5) monitoring the pH within the microbial electrochemical reactor (23) to maintain the pH between 7.5 and 8.2;
6) the hydraulic retention time is 23h, wherein the water inlet and the water outlet are respectively kept still for 15 min.
8. The method according to claim 7, wherein in step 1), the inoculated sludge is anaerobic activated sludge, anoxic activated sludge and anaerobic ammonia oxidation sludge in a volume ratio of 5:4: 1; the MLSS value of the inoculated sludge is 2000-4000 mg/L.
9. The method as claimed in claim 7, wherein in the step 2), after the nitrogen-containing sewage is subjected to oxygen stripping, the DO value is less than 0.5mg/L, and the pH is adjusted to be between 7.5 and 8.2 by adding acid and alkali.
10. The method according to claim 7, wherein in step 3), the average ammonia nitrogen content of the influent nitrogen-containing sewage is 50mg/L, and the average nitrite nitrogen content is 66 mg/L.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111067737.1A CN113683188B (en) | 2021-09-13 | 2021-09-13 | Method and device for electrochemically domesticating anaerobic ammonium oxidation bacteria |
| PCT/CN2021/120951 WO2023035339A1 (en) | 2021-09-13 | 2021-09-27 | Method and device for electrochemically domesticating anaerobic ammonia oxidizing bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111067737.1A CN113683188B (en) | 2021-09-13 | 2021-09-13 | Method and device for electrochemically domesticating anaerobic ammonium oxidation bacteria |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113683188A true CN113683188A (en) | 2021-11-23 |
| CN113683188B CN113683188B (en) | 2022-08-05 |
Family
ID=78586122
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111067737.1A Active CN113683188B (en) | 2021-09-13 | 2021-09-13 | Method and device for electrochemically domesticating anaerobic ammonium oxidation bacteria |
Country Status (2)
| Country | Link |
|---|---|
| CN (1) | CN113683188B (en) |
| WO (1) | WO2023035339A1 (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114149086A (en) * | 2021-11-29 | 2022-03-08 | 中南大学 | Enrichment method of anaerobic ammonium oxidation bacteria, ammonia nitrogen removal method and device |
| CN115261261A (en) * | 2022-06-23 | 2022-11-01 | 浙江巨能环境工程有限公司 | Autotrophic ammonia oxidizing bacteria enrichment method |
| CN115520962A (en) * | 2022-07-05 | 2022-12-27 | 济南大学 | With NO 3- Domestication culture method of electroactive anaerobic ammonium oxidation microorganisms serving as single nitrogen source |
| CN116239213A (en) * | 2023-02-23 | 2023-06-09 | 北京工业大学 | Electrochemical-anaerobic ammonia oxidation device and operation method thereof |
| CN116947208A (en) * | 2023-07-26 | 2023-10-27 | 盐城工学院 | Anaerobic ammonia oxidation rapid starting and directional stimulation process capable of controlling potential step |
| CN115520962B (en) * | 2022-07-05 | 2024-05-03 | 济南大学 | With NO3-Method for domesticating and culturing electroactive anaerobic ammonia oxidizing microorganism serving as single nitrogen source |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102336472A (en) * | 2011-09-08 | 2012-02-01 | 大连理工大学 | Electrically enhanced ANAMMOX biologic nitrogen removal method |
| CN102701445A (en) * | 2012-07-02 | 2012-10-03 | 哈尔滨工业大学 | Normal-temperature starting domestication method for anaerobic ammoxidation reactor with low-dissolved-oxygen inflow water |
| WO2018011413A1 (en) * | 2016-07-15 | 2018-01-18 | Paques I.P. B.V. | Process for the treatment of wastewater containing organic material and ammonia |
| CN109052578A (en) * | 2018-08-22 | 2018-12-21 | 江南大学 | A kind of preparation method of modified electrode and its method for continuous flow bioelectricity Fenton system processing waste water |
| CN209210810U (en) * | 2018-08-08 | 2019-08-06 | 徐廷桢 | A kind of fast culture and domesticating device of anaerobic ammonia oxidizing bacteria |
| CN112142204A (en) * | 2020-09-23 | 2020-12-29 | 沈阳大学 | Method for treating sewage by enrichment culture of anaerobic ammonium oxidation bacteria |
| CN113277613A (en) * | 2021-06-09 | 2021-08-20 | 叶訚 | Electrode regulation-based integrated bioautotrophic denitrification system, method and application |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104528946B (en) * | 2014-12-07 | 2016-06-01 | 北京工业大学 | When a kind of low ratio of carbon to ammonium sanitary sewage, startup take H.hydrossis as the method for advantage limited filamentous sludge bulking fast |
| CN105810984A (en) * | 2016-04-28 | 2016-07-27 | 北京化工大学 | Membrane-less microbial fuel cell electrode device capable of combining wastewater treatment process |
| US20210317018A1 (en) * | 2018-08-30 | 2021-10-14 | Greg Wanger | Systems and methods for remediating aquaculture sediment |
| CN109638327B (en) * | 2018-12-19 | 2021-05-18 | 大连理工大学 | Process for denitrification and power generation by using single-chamber anaerobic ammonia oxidation sludge-microbial fuel cell device |
| CN112551680A (en) * | 2020-12-02 | 2021-03-26 | 佛山市南海区苏科大环境研究院 | Culture enrichment device and method for degrading DMAC (dimethylacetamide) electrochemical active bacteria |
-
2021
- 2021-09-13 CN CN202111067737.1A patent/CN113683188B/en active Active
- 2021-09-27 WO PCT/CN2021/120951 patent/WO2023035339A1/en unknown
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102336472A (en) * | 2011-09-08 | 2012-02-01 | 大连理工大学 | Electrically enhanced ANAMMOX biologic nitrogen removal method |
| CN102701445A (en) * | 2012-07-02 | 2012-10-03 | 哈尔滨工业大学 | Normal-temperature starting domestication method for anaerobic ammoxidation reactor with low-dissolved-oxygen inflow water |
| WO2018011413A1 (en) * | 2016-07-15 | 2018-01-18 | Paques I.P. B.V. | Process for the treatment of wastewater containing organic material and ammonia |
| CN209210810U (en) * | 2018-08-08 | 2019-08-06 | 徐廷桢 | A kind of fast culture and domesticating device of anaerobic ammonia oxidizing bacteria |
| CN109052578A (en) * | 2018-08-22 | 2018-12-21 | 江南大学 | A kind of preparation method of modified electrode and its method for continuous flow bioelectricity Fenton system processing waste water |
| CN112142204A (en) * | 2020-09-23 | 2020-12-29 | 沈阳大学 | Method for treating sewage by enrichment culture of anaerobic ammonium oxidation bacteria |
| CN113277613A (en) * | 2021-06-09 | 2021-08-20 | 叶訚 | Electrode regulation-based integrated bioautotrophic denitrification system, method and application |
Non-Patent Citations (2)
| Title |
|---|
| 杜伟娜著: "《可再生的碳源-生物质能》", 31 August 2015, 北京工业大学出版社, pages: 229 - 231 * |
| 祖波等: "《强化废水生物脱氮新技术：厌氧氨氧化、甲烷化、反硝化耦合的机理与动力学研究》", 31 January 2009, 北京：中国环境科学出版社, pages: 36 * |
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114149086A (en) * | 2021-11-29 | 2022-03-08 | 中南大学 | Enrichment method of anaerobic ammonium oxidation bacteria, ammonia nitrogen removal method and device |
| CN114149086B (en) * | 2021-11-29 | 2023-01-03 | 中南大学 | Enrichment method of anaerobic ammonium oxidation bacteria, ammonia nitrogen removal method and device |
| CN115261261A (en) * | 2022-06-23 | 2022-11-01 | 浙江巨能环境工程有限公司 | Autotrophic ammonia oxidizing bacteria enrichment method |
| CN115261261B (en) * | 2022-06-23 | 2023-12-12 | 浙江巨能环境工程有限公司 | Autotrophic ammonia oxidizing bacteria enrichment method |
| CN115520962A (en) * | 2022-07-05 | 2022-12-27 | 济南大学 | With NO 3- Domestication culture method of electroactive anaerobic ammonium oxidation microorganisms serving as single nitrogen source |
| CN115520962B (en) * | 2022-07-05 | 2024-05-03 | 济南大学 | With NO3-Method for domesticating and culturing electroactive anaerobic ammonia oxidizing microorganism serving as single nitrogen source |
| CN116239213A (en) * | 2023-02-23 | 2023-06-09 | 北京工业大学 | Electrochemical-anaerobic ammonia oxidation device and operation method thereof |
| CN116239213B (en) * | 2023-02-23 | 2023-10-24 | 北京工业大学 | Electrochemical-anaerobic ammonia oxidation device and operation method thereof |
| CN116947208A (en) * | 2023-07-26 | 2023-10-27 | 盐城工学院 | Anaerobic ammonia oxidation rapid starting and directional stimulation process capable of controlling potential step |
| CN116947208B (en) * | 2023-07-26 | 2024-01-02 | 盐城工学院 | Anaerobic ammonia oxidation rapid starting and directional stimulation process capable of controlling potential step |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113683188B (en) | 2022-08-05 |
| WO2023035339A1 (en) | 2023-03-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN113683188B (en) | Method and device for electrochemically domesticating anaerobic ammonium oxidation bacteria | |
| CN103086508B (en) | Microbial fuel cell wastewater treatment system for improving nitrogen removal effect | |
| CN105293716B (en) | A kind of microbiological fuel cell and its method for handling waste water | |
| CN107032479B (en) | Method for strengthening treatment of high-concentration ciprofloxacin wastewater by bioelectrochemical anaerobic/microelectrolysis blending coupling | |
| CN103288213B (en) | Whole-process autotrophic nitrogen removal method and device | |
| CN111661924A (en) | System and method for sulfur autotrophic short-cut denitrification coupling anaerobic ammonia oxidation denitrification | |
| CN105859054A (en) | Device and method for electrochemically treating sewage with microorganisms and microbial film culture method | |
| CN106207208A (en) | A kind of microbiological fuel cell and the application in denitrogenation of waste water thereof | |
| CN112694166A (en) | Process method for enhancing nitrogen removal of sewage with low carbon-nitrogen ratio by using electrode biological carrier | |
| CN112573667A (en) | Sewage treatment device and method based on phycomycete symbiotic electrochemical system | |
| CN103861463A (en) | Electrochemically assisted biological denitrification method of source separated urine | |
| CN113023870A (en) | Process for realizing short-cut denitrification series anaerobic ammonia oxidation treatment of high-concentration nitrate and ammonia nitrogen wastewater by using sludge fermentation liquor as carbon source | |
| CN104868146A (en) | Microbial fuel cell for treating domestic sewage and producing electricity by coupling A<2>/O technology | |
| CN102646843A (en) | Method for leaching Cobalt (III) (Co (III)) of lithium cobalt oxide in chemical cathode microbial fuel cell | |
| CN102249424A (en) | Proton exchange membrane-free microbiological fuel cell sewage treatment system and application method thereof | |
| CN205740505U (en) | Microorganism electrochemical is utilized to process the device of sewage | |
| CN112447985B (en) | Preparation method of microbial fuel cell electrode for fixing microbial flora | |
| CN103715444B (en) | Batch-type electrode reversal microbial fuel cell and application thereof | |
| CN113023869A (en) | Process for treating high-concentration nitrate wastewater and domestic sewage by using sludge fermentation liquor as carbon source and by using short-range denitrification tandem anaerobic ammonia oxidation | |
| CN109250805B (en) | Optical microbial fuel cell for simultaneously treating high-concentration starch wastewater and carbon-source-free nitrate-containing wastewater and application thereof | |
| CN110615525B (en) | Method for enriching anaerobic ammonium oxidation bacteria and application | |
| JP2009061390A (en) | Direct oxidation method of ammonia nitrogen in water and its apparatus | |
| CN114149086B (en) | Enrichment method of anaerobic ammonium oxidation bacteria, ammonia nitrogen removal method and device | |
| CN113683192B (en) | Device and method for culturing and enriching short-cut denitrification granular sludge for providing electron acceptor nitrite nitrogen for anaerobic ammonia oxidation | |
| CN214400167U (en) | Sewage treatment device based on algae bacterium symbiosis electrochemical system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |