CN115520962B - With NO3-Method for domesticating and culturing electroactive anaerobic ammonia oxidizing microorganism serving as single nitrogen source - Google Patents
With NO3-Method for domesticating and culturing electroactive anaerobic ammonia oxidizing microorganism serving as single nitrogen source Download PDFInfo
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- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 title claims abstract description 134
- 229910021529 ammonia Inorganic materials 0.000 title claims abstract description 67
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 title claims abstract description 66
- 244000005700 microbiome Species 0.000 title claims abstract description 61
- 229910052757 nitrogen Inorganic materials 0.000 title claims abstract description 35
- 230000001590 oxidative effect Effects 0.000 title claims description 19
- 238000000034 method Methods 0.000 title claims description 10
- 238000012258 culturing Methods 0.000 title claims description 7
- 238000007254 oxidation reaction Methods 0.000 claims abstract description 44
- 230000003647 oxidation Effects 0.000 claims abstract description 41
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 26
- 238000006243 chemical reaction Methods 0.000 claims abstract description 25
- 230000000694 effects Effects 0.000 claims abstract description 16
- NHNBFGGVMKEFGY-UHFFFAOYSA-N Nitrate Chemical compound [O-][N+]([O-])=O NHNBFGGVMKEFGY-UHFFFAOYSA-N 0.000 claims abstract description 10
- 230000005684 electric field Effects 0.000 claims abstract description 10
- 238000012136 culture method Methods 0.000 claims abstract description 6
- 239000010865 sewage Substances 0.000 claims abstract description 6
- 239000000758 substrate Substances 0.000 claims abstract description 6
- 239000010802 sludge Substances 0.000 claims description 20
- 238000003756 stirring Methods 0.000 claims description 10
- IOVCWXUNBOPUCH-UHFFFAOYSA-M Nitrite anion Chemical compound [O-]N=O IOVCWXUNBOPUCH-UHFFFAOYSA-M 0.000 claims description 7
- 230000014759 maintenance of location Effects 0.000 claims description 6
- 230000037361 pathway Effects 0.000 claims description 6
- 238000005189 flocculation Methods 0.000 claims description 5
- 230000016615 flocculation Effects 0.000 claims description 5
- 235000003642 hunger Nutrition 0.000 claims description 5
- 230000005284 excitation Effects 0.000 claims description 4
- 241000894006 Bacteria Species 0.000 claims description 3
- 229930182555 Penicillin Natural products 0.000 claims description 3
- JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 claims description 3
- 239000008187 granular material Substances 0.000 claims description 3
- 239000002054 inoculum Substances 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 229940049954 penicillin Drugs 0.000 claims description 3
- 238000004062 sedimentation Methods 0.000 claims description 3
- 230000037351 starvation Effects 0.000 claims description 3
- LXAHHHIGZXPRKQ-UHFFFAOYSA-N 5-fluoro-2-methylpyridine Chemical compound CC1=CC=C(F)C=N1 LXAHHHIGZXPRKQ-UHFFFAOYSA-N 0.000 claims description 2
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 claims description 2
- 239000007853 buffer solution Substances 0.000 claims description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N iron Substances [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims description 2
- 229910052742 iron Inorganic materials 0.000 claims description 2
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 claims 2
- 238000009825 accumulation Methods 0.000 claims 1
- 239000011159 matrix material Substances 0.000 claims 1
- 230000001502 supplementing effect Effects 0.000 claims 1
- 238000009210 therapy by ultrasound Methods 0.000 claims 1
- 230000001651 autotrophic effect Effects 0.000 abstract description 7
- 229910002651 NO3 Inorganic materials 0.000 abstract description 3
- 230000001925 catabolic effect Effects 0.000 abstract description 3
- 230000037353 metabolic pathway Effects 0.000 abstract description 3
- 238000012851 eutrophication Methods 0.000 description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 3
- 230000010718 Oxidation Activity Effects 0.000 description 3
- MMDJDBSEMBIJBB-UHFFFAOYSA-N [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] Chemical compound [O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O.[NH6+3] MMDJDBSEMBIJBB-UHFFFAOYSA-N 0.000 description 3
- 229910052799 carbon Inorganic materials 0.000 description 3
- 239000005431 greenhouse gas Substances 0.000 description 2
- JVMRPSJZNHXORP-UHFFFAOYSA-N ON=O.ON=O.ON=O.N Chemical compound ON=O.ON=O.ON=O.N JVMRPSJZNHXORP-UHFFFAOYSA-N 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010349 cathodic reaction Methods 0.000 description 1
- SMZGWLOIMKISPD-UHFFFAOYSA-J disodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate;iron(2+) Chemical compound [Na+].[Na+].[Fe+2].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O SMZGWLOIMKISPD-UHFFFAOYSA-J 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000027756 respiratory electron transport chain Effects 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/005—Combined electrochemical biological processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/28—Anaerobic digestion processes
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/10—Inorganic compounds
- C02F2101/16—Nitrogen compounds, e.g. ammonia
- C02F2101/163—Nitrates
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- Life Sciences & Earth Sciences (AREA)
- Microbiology (AREA)
- Chemical & Material Sciences (AREA)
- Water Supply & Treatment (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Engineering & Computer Science (AREA)
- Environmental & Geological Engineering (AREA)
- Organic Chemistry (AREA)
- Electrochemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Molecular Biology (AREA)
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- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Water Treatment By Electricity Or Magnetism (AREA)
Abstract
The invention relates to the field of sewage treatment, and provides an electroactive anaerobic ammonia oxidation microorganism domestication culture method taking NO 3 ‑ as a single nitrogen source. Firstly, an external power supply mode is used for domesticating anaerobic ammonia oxidation microorganisms with electric activity, and then catabolic nitrate of the electroactive anaerobic ammonia oxidation microorganisms is excited to reduce into an ammonia metabolic pathway through substrate domestication, so that the anaerobic ammonia oxidation autotrophic denitrification is realized by taking electric field cathode electrons as electron donors and NO 3 ‑ as a single nitrogen source. The invention realizes the anaerobic ammoxidation reaction under the single NO 3 ‑ nitrogen source mode, achieves the aim of autotrophic removal of NO 3 ‑ in the water body, and the domesticated electroactive anaerobic ammoxidation microorganism taking NO 3 ‑ as the single nitrogen source has the direct removal rate of NO 3 ‑ in the water body of more than 75 percent and the specific anaerobic ammoxidation activity of more than 0.3 g-N/(g ‑1-VSS·d‑1).
Description
Technical Field
The invention belongs to the technical field of sewage treatment, and relates to an electroactive anaerobic ammonia oxidation microorganism domestication culture method taking NO 3 - as a single nitrogen source.
Background
The eutrophication phenomenon of water body is still one of the most serious environmental problems worldwide, and anaerobic ammonia oxidation is the sewage biological denitrification technology with the most popularization and application value. The traditional anaerobic ammonia oxidation microorganism takes nitrite nitrogen NO 2 - as an electron acceptor, and oxidizes ammonia nitrogen into N 2 under the anoxic condition, so that autotrophic removal of nitrogen in the water body is realized, the problems of organic carbon source consumption and greenhouse gas N 2 O emission existing in the traditional denitrification can be avoided, but nitrate nitrogen NO 3 - stably existing in the water body still cannot be directly and effectively removed.
Disclosure of Invention
The electron reduction NO 3 - provided by the electric field cathode in the anaerobic ammonia oxidation microorganism with electric activity is used for accumulating NO 2 -, and the electron of the cathode is used for replacing the position of NH 4 + electron donor, so that the anaerobic ammonia oxidation reaction under a single NO 3 - nitrogen source mode is realized, the autotrophic removal of NO 3 - in the water body is further realized, and the problem of water body eutrophication is solved.
The technical scheme of the invention is as follows:
An electro-active anaerobic ammonia oxidation microorganism domestication culture method taking NO 3 - as a single nitrogen source comprises the following steps:
① Pretreatment of inoculated sludge: anaerobic ammoxidation sludge granular sludge is used as an inoculum, after starvation pretreatment, the granular disintegration and flocculation are promoted by ultrasound, and flocculent sludge is inoculated into a cathode reaction chamber of a double-cavity electrolytic cell for domestication culture after sedimentation and concentration.
Further, the particle size of the granular sludge is 1.0-3.0 mm, and after 5-15 and d hunger pretreatment, ultrasonic 1h promotes the disintegration and flocculation of the granules;
② And (3) controlling the working condition of domestication and culture of the electroactive anaerobic ammonia oxidation microorganism: the concentration of flocculent sludge in the cathode reaction chamber is controlled to be 2500-4000 mg/L, 0.5% penicillin is added to inhibit the growth of heterotrophic bacteria, ar and CO 2 are introduced to create an anaerobic environment, the initial concentration of NH 4 + and NO 2 - is respectively 100 mg-N/L and 120 mg-N/L, the continuous mode operation is carried out, the Hydraulic Retention Time (HRT) is 12 h, naHCO 3 and microelements are supplemented every 2 d to supply anaerobic ammonia oxidizing microorganisms for growth, the load is increased by 25% every 10 d as a period, and stirring paddles are used for stirring to ensure uniform mass transfer in the system.
Further, the addition ratio of Ar to CO 2 was 95%:5, stirring paddles stir 10min every 60 min to ensure uniform mass transfer in the system.
When the concentrations of the water inflow NH 4 + and the NO 2 - in the cathode reaction chamber reach 300 mg-N/L and 360 mg-N/L respectively and the total nitrogen removal rate is stabilized to be more than 75%, the NH 4 + proportion is gradually reduced to be 0 in a 25% range, so that a reaction path for generating N 2 by reducing the NO 2 - by cathode electrons is constructed, and the anaerobic ammonia oxidizing microorganism with electric activity is domesticated.
③ Control of conditions in the pathway to excite cathodic electrons to supply electroactive anaerobic ammonia oxidizing microorganisms to reduce nitrate (NO 3 -) and accumulate nitrite (NO 2 -): in the cathode reaction chamber for successfully domesticating electroactive microorganisms, changing substrate components, taking NO 3 - as a nitrogen source, and simultaneously adding 1% of EDTA disodium iron to promote excitation of DNRA pathway;
The initial concentration of NO 3 - is 120 mg-N/L, the operation is continuous, the Hydraulic Retention Time (HRT) is 12 h, naHCO 3 and microelements are supplemented every 2d and supplied to the growth of the anaerobic ammonia oxidation microorganism, the load is lifted by 25% every 10 d as a period, when the concentration of the inflow NO 3 - of the system reaches 360 mg-N/L, the removal rate of NO 3 - is stabilized above 75%, the way that the electro-active anaerobic ammonia oxidation microorganism reduces nitrate (NO 3 -) to accumulate nitrite (NO 2 -) is excited, and the cathode electron is further utilized to reduce NO 2 - to generate N 2, so that the effective removal of the electro-active anaerobic ammonia oxidation microorganism to NO 3 - in the water body is realized, namely the electro-active anaerobic ammonia oxidation microorganism which takes NO 3 - as a single nitrogen source is successfully domesticated, and the ratio anaerobic ammonia oxidation activity can reach above 0.3 g-N/(g -1-VSS·d-1).
The anaerobic ammonia oxidation microorganism with the electric activity adopts a double-cavity electrolytic cell as a reaction device, the control voltage range of a direct current stabilized power supply is 0.8-1.2V, and the acclimation and enrichment of the anaerobic ammonia oxidation microorganism with the electric activity are carried out in a cathode reaction cell.
Further, the double-cavity electrolytic cell comprises a cathode reaction chamber, an anode chamber, a direct-current stabilized power supply and a salt bridge, wherein two ends of the salt bridge are respectively inserted into the cathode reaction chamber and the anode chamber, an electric field cathode and a reference electrode of the direct-current stabilized power supply are arranged in the cathode reaction chamber, an electric field anode is arranged in the anode chamber, a KCl buffer solution is filled in the anode chamber, the cathode reaction chamber is used for domesticating and culturing electroactive anaerobic ammonia oxidizing microorganisms, and a stirrer is arranged in the cathode reaction chamber;
The anaerobic ammonia oxidizing microorganism with electric activity is domesticated and cultured by taking NO 3 - as a single nitrogen source, and is applied to the removal of nitrate nitrogen in sewage, and the steps are as follows: firstly domesticating and culturing anaerobic ammonia oxidation microorganisms with electric activity, and then exciting catabolic nitrate of the electric activity anaerobic ammonia oxidation microorganisms to reduce into ammonia (DNRA) metabolic pathway through substrate domestication to realize anaerobic ammonia oxidation autotrophic denitrification by taking electric field cathode electrons as electron donors and NO 3 - as a single nitrogen source.
The invention has the beneficial effects that:
The anaerobic ammonia oxidation microorganism with electric activity is domesticated by the method, so that the anaerobic ammonia oxidation microorganism accumulates NO 2 - by utilizing electrons provided by an electric field cathode to reduce NO 3 -, and utilizes the cathode electrons to replace the position of NH 4 + electron donor, thereby realizing anaerobic ammonia oxidation reaction under a single NO 3 - nitrogen source mode and further achieving autotrophic removal of NO 3 - in water.
The invention uses nitrate nitrogen (NO 3 -) as the electroactive anaerobic ammonia oxidizing microorganism of single nitrogen source, is used for removing NO 3 - in water, avoids the problems of organic carbon source consumption and greenhouse gas N 2 O emission existing in the traditional denitrification, and has positive significance for solving the problem of water eutrophication.
The method comprises three steps of inoculation sludge pretreatment, working condition control of domestication culture of the electroactive anaerobic ammonia oxidation microorganisms, and working condition control of activating cathode electrons to supply the electroactive anaerobic ammonia oxidation microorganisms to reduce nitrate (NO 3 -) and accumulate nitrite (NO 2 -), and various parameters are matched, so that the removal rate of NO 3 - in water body can reach 75% by the electroactive anaerobic ammonia oxidation microorganisms which are successfully domesticated under the synergistic effect and take NO 3 - as a single nitrogen source, and the specific anaerobic ammonia oxidation activity can reach more than 0.3 g-N/(g -1-VSS·d-1).
Drawings
Inlet and outlet water index and total nitrogen removal rate at acclimatization stage of FIG. 1
FIG. 2 effect of electroactive anaerobic ammonia oxidizing microorganisms on removal of single NO 3
Detailed Description
Example 1 an electroactive anaerobic ammonia oxidizing microorganism acclimation culture method with NO 3 - as a single nitrogen source, comprising the steps of:
① Pretreatment of inoculated sludge: anaerobic ammoxidation sludge granular sludge is used as an inoculum, the grain size of the granular sludge is 1.0-3.0 mm, after 15 d hunger pretreatment, ultrasonic 1h promotes the disintegration and flocculation of the granules, after sedimentation and concentration, 400 ml flocculent sludge (MLSS about 3000-3500 mg/L) is inoculated into a cathode reaction chamber of a double-cavity electrolytic cell with the volume of 1L for domestication culture, and the direct-current stabilized power supply control voltage range of the double-cavity electrolytic cell is between 0.8-1.2V.
② And (3) controlling the working condition of domestication and culture of the electroactive anaerobic ammonia oxidation microorganism: controlling the concentration of flocculent sludge in a cathode reaction chamber to 2500-4000 mg/L, adding 0.5% penicillin to inhibit the growth of heterotrophic bacteria, introducing Ar and CO 2 to create an anaerobic environment, wherein the adding ratio of Ar to CO 2 is 95%:5%, the initial NH 4 + and NO 2 - concentrations are respectively 100 mg-N/L and 120 mg-N/L, the continuous mode operation is carried out, the Hydraulic Retention Time (HRT) is 12h, naHCO 3 and microelements are supplemented every 2d and supplied to the growth of anaerobic ammonia oxidizing microorganisms, the load is lifted by 25% every 10 d as a period, and the stirring paddles stir 10min every 60min to ensure uniform mass transfer in the system.
As shown in FIG. 1, after the reactor is operated to 40 d th, the concentration of the inlet water NH 4 + and the concentration of the inlet water NO 2 - respectively reach 300 mg-N/L and 360 mg-N/L, and the total nitrogen removal rate is stabilized to be more than 75%, the reactor is stably operated for 20 d. From 60 d, the NH 4 + ratio is gradually reduced to 0 in a range of 25% under the premise of keeping the mass conservation of the substrate nitrogen, and the total nitrogen removal rate is still above 75%, which shows that the reaction path of generating N 2 by cathodic electron reduction NO 2 - is constructed, and the anaerobic ammonia oxidizing microorganism with electric activity is domesticated.
③ Control of conditions in the pathway to excite cathodic electrons to supply electroactive anaerobic ammonia oxidizing microorganisms to reduce nitrate (NO 3 -) and accumulate nitrite (NO 2 -): in a cathodic reaction chamber where electroactive microorganisms were successfully acclimatized, the substrate composition was changed with NO 3 - as the nitrogen source, while 1% disodium iron EDTA was added to promote excitation of the DNRA pathway.
As shown in FIG. 2, the initial water inlet NO 3 - is 120 mg-N/L, the continuous mode operation is carried out, the Hydraulic Retention Time (HRT) is 12 h, naHCO 3 and microelements are supplemented every 2 d and are supplied to the growth of the anaerobic ammonia oxidation microorganism, the load is lifted by 25% every 10 d as one period, the concentration of the water inlet NO 3 - from the 50 th d system reaches 360 mg-N/L, the NO 3 - removal rate is stabilized to be more than 75%, the phenomenon shows that the way of reducing nitrate (NO 3 -) by the electroactive anaerobic ammonia oxidation microorganism to accumulate nitrite (NO 2 -) is excited, the NO 2 - can be successfully reduced by cathode electrons to generate N 2, and the effective removal of NO 3 - in the water body by the electroactive anaerobic ammonia oxidation microorganism is realized, namely, the electroactive anaerobic ammonia oxidation microorganism taking NO 3 - as a single nitrogen source is successfully domesticated, and the specific anaerobic ammonia oxidation activity can reach more than 0.3 g-N/(g -1-VSSd-1).
The experimental process shows that the anaerobic ammonia oxidizing microorganism with electric activity exists, extracellular electron transfer can be realized through an external electric field, and under the condition of sufficient electron donor, the metabolic pathway of the catabolic nitrate of the anaerobic ammonia oxidizing microorganism for reduction into ammonia (DNRA) can be excited, NO 3 - is reduced, NO 2 - is accumulated, and therefore NO 3 - in the water body is removed. Therefore, the method for domesticating and culturing the electroactive anaerobic ammonia oxidation microorganism taking NO 3 - as a single nitrogen source can effectively remove NO 3 - in the water body, has positive significance for solving the problem of eutrophication of the water body, and has better treatment effect especially for treating sewage with low carbon-nitrogen ratio without adding any additional carbon source.
The invention domesticates the anaerobic ammonia oxidation microorganism with electric activity, utilizes electrons provided by an electric field cathode to reduce NO 3 - to accumulate NO 2 -, utilizes cathode electrons to replace the position of NH 4 + electron donor, realizes anaerobic ammonia oxidation reaction under a single NO 3 - nitrogen source mode, and further achieves autotrophic removal of NO 3 - in water.
The foregoing describes one embodiment of the present invention in detail, but the description is only a preferred embodiment of the present invention and should not be construed as limiting the scope of the invention. All equivalent changes and modifications within the scope of the present invention are intended to be covered by the present invention.
Claims (4)
1. An electro-active anaerobic ammonia oxidation microorganism domestication culture method taking NO 3 - as a single nitrogen source is characterized in that: the method comprises the following steps:
① Pretreatment of inoculated sludge: anaerobic ammonia oxidation sludge granular sludge is used as an inoculum, after starvation pretreatment, ultrasonic promotion of particle disintegration and flocculation is carried out, flocculent sludge is inoculated into a cathode reaction chamber of a double-cavity electrolytic cell after sedimentation and concentration, the double-cavity electrolytic cell comprises a cathode reaction chamber, an anode chamber, a direct-current stabilized power supply and a salt bridge, two ends of the salt bridge are respectively inserted into the cathode reaction chamber and the anode chamber, an electric field cathode and a reference electrode of the direct-current stabilized power supply are arranged in the cathode reaction chamber, an electric field anode is arranged in the anode chamber, KCl buffer solution is filled in the anode chamber, and the cathode reaction chamber is used for domestication culture of electroactive anaerobic ammonia oxidation microorganisms;
② Domestication culture of electroactive anaerobic ammonia oxidizing microorganisms: controlling the concentration of flocculent sludge in the cathode reaction chamber to 2500-4000 mg/L, adding 0.5% penicillin to inhibit the growth of heterotrophic bacteria, introducing Ar and CO 2 to create anaerobic environment, enabling the initial concentration of NH 4 + and NO 2 - to be 100 mg-N/L and 120 mg-N/L respectively, running in a continuous mode, enabling the hydraulic retention time to be 12 h, supplementing NaHCO 3 and microelements to every 2d to supply anaerobic ammonia oxidizing microorganisms for growth, and lifting the load by 25% every 10 d as a period, and stirring by a stirring paddle to ensure uniform mass transfer in the system;
Gradually reducing the NH 4 + ratio to 0 in a range of 25% on the premise of keeping the mass conservation of matrix nitrogen when the concentrations of the inlet water NH 4 + and NO 2 - in the cathode reaction chamber reach 300 mg-N/L and 360 mg-N/L respectively and the total nitrogen removal rate is more than 75%, so as to construct an N 2 reaction path generated by cathode electron reduction of NO 2 -, and domesticating anaerobic ammonia oxidizing microorganisms with electric activity;
③ Excitation of cathodic electrons supply the electroactive anaerobic ammonia oxidizing microorganism to reduce nitrate (NO 3 -) accumulation nitrite (NO 2 -) pathway: in the cathode reaction chamber for successfully domesticating electroactive microorganisms, changing substrate components, taking NO 3 - as a nitrogen source, and simultaneously adding 1% of EDTA disodium iron to promote excitation of DNRA pathway;
The initial concentration of NO 3 - is 120 mg-N/L, the operation is continuous, the Hydraulic Retention Time (HRT) is 12 h, naHCO 3 and microelements are supplemented every 2d and supplied to the growth of the anaerobic ammonia oxidation microorganism, the load is lifted by 25% every 10 d as a period, when the concentration of the inflow water NO 3 - of the system reaches 360 mg-N/L, the removal rate of NO 3 - is stabilized to be more than 75%, the way that the electro-active anaerobic ammonia oxidation microorganism reduces nitrate (NO 3 -) to accumulate nitrite (NO 2 -) is excited, and the cathode electron is further utilized to reduce NO 2 - to generate N 2, so that the electro-active anaerobic ammonia oxidation microorganism in water body NO 3 - is effectively removed, namely the electro-active anaerobic ammonia oxidation microorganism which takes NO 3 - as a single nitrogen source is successfully domesticated.
2. The method for domesticating and culturing an electroactive anaerobic ammonium oxidation microorganism using NO 3 - as a single nitrogen source according to claim 1, wherein the method comprises the steps of: in the step ①, the particle size of the granular sludge is 1.0-3.0 mm, and after starvation pretreatment of 5-15 and d, the granular sludge is subjected to ultrasonic treatment for 1h to promote disintegration and flocculation of the granules;
In step ②, the addition ratio of Ar to CO 2 is 95%:5, stirring paddles stir 10min every 60min to ensure uniform mass transfer in the system.
3. The method for domesticating and culturing an electroactive anaerobic ammonium oxidation microorganism using NO 3 - as a single nitrogen source according to claim 1 or 2, wherein: the control voltage range of the direct current stabilized power supply in the double-cavity electrolytic cell is 0.8-1.2V.
4. Use of a microorganism obtained by the method for domesticating and culturing an electroactive anaerobic ammonia oxidizing microorganism using NO 3 - as a single nitrogen source according to any one of claims 1 to 3 for treating sewage with a low carbon nitrogen ratio.
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| CN111573821A (en) * | 2020-05-22 | 2020-08-25 | 盐城工学院 | Electrode denitrification system based on autotrophic short-cut denitrification-anaerobic ammonia oxidation module |
| CN112939213A (en) * | 2021-04-01 | 2021-06-11 | 北京工业大学 | Rapid CANON granular sludge starting method based on hydraulic screening intermittent starvation |
| CN113683188A (en) * | 2021-09-13 | 2021-11-23 | 江苏大学 | Method and device for electrochemically domesticating anaerobic ammonium oxidation bacteria |
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